KR101708459B1 - Cosmetic composition for moisturizing and elasiticity of skin containing the LAMINARIA JAPONICA extract, UNDARIA PINNATIFIDA extract and PLANTAGO ASIATICA SEED extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of sea tangle, an extract of sea mustard and a tea extract as an active ingredient. More particularly, the present invention relates to a cosmetic composition which contains an extract of sea tangle, an extract of sea mustard and a tea extract as an active ingredient to improve the moisturizing power of the skin and enhance the elasticity of the skin.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin moisturizing and elasticity containing extracts of sea tangle, seaweed extract and tea extract, and to a cosmetic composition for skin moisturizing and elasticity,

The present invention relates to a cosmetic composition containing an extract of sea tangle, an extract of sea mustard and a tea extract as an active ingredient. More particularly, the present invention relates to a cosmetic composition which contains an extract of sea tangle, an extract of sea mustard and a tea extract as an active ingredient to improve the moisturizing power of the skin and enhance the elasticity of the skin.

The skin is largely divided into three layers of epidermis, dermis and subcutaneous adipose tissue in order from the outside. It functions to protect the human body from physical and chemical stimulation from the external environment. Especially, skin has about 65 ~ 70% of water is controlled to evaporate out of the body. Among them, the epidermis is divided into the order of stratum corneum, granular layer, superficial layer, and basal layer from the outside. The stratum corneum of the epidermis contains about 10 to 20% of water and exists in the outermost part of the human body to suppress water evaporation from the body ( J. Invest . Dermatol . 80 ( Suppl .), 44-49, 1983 ). The cells that constitute these stratum corneum have a high concentration of natural moisturizing factor (NMF), which is a water-soluble component, which helps the skin to exhibit flexibility and to maintain proper moisture ( J. Invest . Dermatol . 54, 24-31, 1970 ). However, due to changes in the environment or changes in life patterns, various stresses and environmental pollution in social life, frequent cleansing due to makeup habits, and aging of natural skin due to age increase, The surface becomes rough, the skin becomes lustrous and appears dull, and the problem of skin moisturization becomes more and more important.

As the skin ages, the elasticity degradation phenomenon is caused by the ultraviolet ray photoaging, the increase in the age and the endogenous factors such as menopause, genetic factors, environmental factors, which leads to reduction of fibroblast cleavage, reduction of collagen synthesis, And the elasticity of the skin is decreased. In particular, the balance of hormones is broken in the forties, and the ability of cell regeneration, collagen synthesis, and skin lipid production, which plays an important role in skin moisturization, is greatly reduced. Research on cosmetics for the purpose of improving the skin elasticity degradation has been actively carried out as consumers' desire to look younger and expectation of cosmetics increase.

In general, cosmetics are intended to clean the body, to make itself beautiful and attractive, and to protect the skin and hair from ultraviolet rays and drying to prevent aging. Recently, with the development of industrial society and the increase of social activities, self-awareness to express oneself positively has increased, and the desire to use cosmetics to keep their own skin and maintain their health is greatly increased. Accordingly, various types and formulations of foundation and color cosmetics have been studied and developed. Among them, skin moisturizing cosmetics and skin elasticity improving cosmetics, which improve skin dryness, are recognized as the most important products in various categories.

Accordingly, various cosmetic raw materials have been developed in order to improve skin moisturization and skin elasticity reduction. In recent years, the use of animal raw materials has been drastically reduced due to the deterioration of consumers' preference for animal raw materials and the excommunication of mad cow disease And raw materials derived from natural plants are attracting attention. In addition, consumers are increasingly increasing their preference for multi-functional cosmetics, such as cosmetics, for example, moisturizers and cosmetics that have an elasticity-enhancing effect as an additional function, thereby developing a multi-functional plant extract It became a very urgent and important issue.

The inventors of the present invention have found that kelp extract, wakame extract, and tea extract extract have the effect of improving the skin moisture and skin elasticity, and have completed the present invention.

Accordingly, an object of the present invention is to provide a skin cosmetic composition having excellent moisture resistance.

Another object of the present invention is to provide a skin cosmetic composition which improves skin elasticity.

In order to achieve the above object, the present invention provides a cosmetic composition for skin moisturizing or skin elasticity improvement comprising an extract of sea tangle, an extract of marine area, and a tea extract as an active ingredient.

The skin cosmetic composition of the present invention contains kelp extract, wakame extract, and tea extract to enhance skin moisturizing power, and also has excellent skin elasticity improving effect.

Fig. 1 is a graph showing changes in skin water volume before and after application of Example 1 and Comparative Example 1. Fig.
Fig. 2 is a graph showing changes in skin elasticity before and after application of Example 1 and Comparative Example 1. Fig.
Fig. 3 is a photograph showing changes in shape of artificial dermis before and after application of Example 1 and Comparative Example 1. Fig.
4 is a graph showing changes in moisture evaporation amount of artificial dermis before and after application of Example 1 and Comparative Example 1. Fig.
FIG. 5 shows changes in occludin expression of tight junctions after application of Example 1 and Comparative Example 1. FIG.

The cosmetic composition of the present invention contains seaweed extract, wakame extract and tea extract.

In particular, the cosmetic composition of the present invention preferably contains a seaweed extract, a sea mustard extract, and a tea extract.

The kelp kelp used in the present invention refers to Laminaria japonica belonging to the brown algae kelp. In the oriental medicine field, dried thalli is called kelp (kelp) and is used as a herbal medicine. Its flavor is woven, . It has the property of eliminating sputum and eosinophilia and making urine come out well, and it is used as a medicine to eliminate cough, asthma and seawater. It also contains a large amount of minerals such as iodine and potassium, which has the effect of promoting metabolism. In <Dongbibogam>, it is written, "It controls the mountain, restores the boil, governs the lump of the hump, and softens the solid."

The seaweed extract used in the present invention is prepared by accurately weighing 50 g of lyophilized kelp and then extracting it by cooling with an organic solvent at room temperature for 48 hours and then evaporating the organic solvent used for extraction completely using an evaporator After concentration, it was used as a sample without further manipulation. At this time, the organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. The process for obtaining the extract is not limited to the above contents.

The skin cosmetic composition of the present invention contains the sea tangle extract in an amount of 0.001 to 10% by weight based on the total weight of the composition. This is because no significant effect can be expected at less than 0.001 wt%, and no significant increase in the effect is observed when the content exceeds 10 wt%.

It is called undaria pinnatifida, which is a year-old fish with seaweed, and is called a sea bream or a sweet potato, a purple vegetable, or a sea bream. In <Dongbu Bohak>, "it is cold and tasteless and poisonous. The efficacy is to remove the stiffness of the fever as it feels, to heal the cohesion, and to make the pee go well. "

The seaweed extract used in the present invention is obtained by accurately weighing 50 g of the lyophilized seaweed and then extracting it by cooling with an organic solvent at room temperature for 48 hours and then evaporating the organic solvent used for extraction completely using an evaporator After concentration, it was used as a sample without further manipulation. At this time, the organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. The process for obtaining the extract is not limited to the above contents.

The composition for external application for skin of the present invention contains the above sea mustard extract in an amount of 0.001 to 10% by weight based on the total weight of the composition. This is because no significant effect can be expected at less than 0.001 wt%, and no significant increase in the effect of increasing the content exceeds 10 wt%.

Tea is a seed of Plantago asiatica L. which is a plant belonging to Plantaginaceae. It is used as a medicine of Ishizu, 清 热, 名 明, And it is mainly used to treat urinary incontinence, epilepsy, hematuria, seawater and intentional (redness). According to <Dongbu Bohak>, "Chae-ee is fragile and heal mainly those that do not come out well, control five kinds of gonorrhea, make urine clogged, let the water excrete well, brighten the eyes It eliminates congestion, and it controls the heat of the liver. " Therefore, secondary electronegal swelling due to the swelling of the type of poison is improved.

Next, the step of obtaining the tea extract used in the present invention is as follows. 50 g of the secondary electrons were weighed accurately and then extracted with an organic solvent at room temperature for 48 hours. The organic solvent used for the extraction was completely evaporated using an evaporator, and then concentrated without further manipulation. Respectively. At this time, the organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. The process for obtaining the extract is not limited to the above contents.

The composition for external application for skin of the present invention contains 0.001 to 10% by weight of the above-described secondary electron extract relative to the total weight of the composition. This is because no significant effect can be expected at less than 0.001 wt%, and no significant increase in the effect of increasing the content exceeds 10 wt%.

The composition for external application for skin according to the present invention contains a cosmetically and dermatologically acceptable medium and / or base. It may be in any form suitable for topical application, for example as a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic form (liposome) In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art. In addition, the composition for external application for skin according to the present invention may be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The composition for external application for skin according to the present invention can be used as a skin external agent composition in the form of a composition containing a lipid, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, Such as cosmetics or skin, such as fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics Adjuvants conventionally used in the scientific field, and the like. These adjuvants are introduced in amounts commonly used in the cosmetic or dermatological fields.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited to these examples.

&Lt; Preparation Example 1 > Preparation of marine kelp extract

50 g of frozen dried seaweed seaweed was weighed accurately and then extracted with 70% ethanol for 48 hours at room temperature. The ethanol used for the extraction was evaporated completely using an evaporator, .

&Lt; Preparation Example 2 >

50 g of the freeze-dried seaweed was weighed accurately and then extracted with 70% ethanol for 48 hours at room temperature. The ethanol used for the extraction was completely evaporated using an evaporator to prepare a sea bream extract Respectively.

&Lt; Preparation Example 3 > Preparation of tea extract

50 g of tea was precisely weighed and then extracted with cold 70% ethanol for 48 hours at room temperature. The ethanol used for the extraction was evaporated completely using an evaporator to obtain a sea mustard extract.

&Lt; Example 1 and Comparative Example 1 >

Example 1 and Comparative Example 1 were prepared in the form of a cream with the composition shown in Table 1 below (unit: wt%).

ingredient Example 1 Comparative Example 1 Production Example 1 1.0 0 Production Example 2 1.0 0 Production Example 3 1.0 0 Purified water Balance Balance glycerin 8.0 8.0 Butylene glycol 4.0 4.0 Hyaluronic acid extract 5.0 5.0 Beta Glucan 7.0 7.0 Carbomer 0.15 0.15 Caprylic / capric triglyceride 8.0 8.0 Squalane 5.0 5.0 Cetearyl glucoside 1.5 1.5 Sorbitan stearate 0.4 0.4 Cetearyl alcohol 2.0 2.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Triethanolamine 0.15 0.15

Test Example 1 Measurement of skin moisture content

The subjects of the evaluation were 30 women aged 30 years old and divided into Example 1 and Comparative Example 1, and the skin moisture content was measured after 2 weeks from the use of Example 1 and Comparative Example 1 on the left and right cheeks, respectively. Specifically, 30 subjects were rinsed with soap and their skin was adapted under constant temperature and humidity conditions (temperature 20 ± 2 ° C., relative humidity 40 ± 2%). The skin surface capacitance was measured three times using a corneometer (CM825). The average results are shown in Fig.

As can be seen from Fig. 1, the water content of the cheek front increased 3% after using the Comparative Example 1 for 2 weeks, and the moisture content of the lower part of the ball, which was particularly dry in the face, increased by 11%, whereas Example 1 was used for 2 weeks Shows that the water content of the cheek front is increased by 10% and the water content of the lower part of the ball is increased by 78%, and the skin moisture amount can be remarkably increased when the embodiment of the present invention is used.

&Lt; Test Example 2 > Measurement of improvement in skin elasticity

The skin elasticity was measured after 30 persons to be evaluated in Test Example 1 were used for Example 1 and Comparative Example 1 for 2 weeks. Specifically, 30 subjects were rinsed with soap and their skin was adapted under constant temperature and humidity conditions (temperature 20 ± 2 ° C., relative humidity 40 ± 2%). The elasticity of the skin was analyzed by measuring the degree of restoration of the skin pulled by the sound pressure on the skin using a Cutometer (MPA580). The results are shown in Fig.

As can be seen from FIG. 2, the skin elasticity was increased by 0.1% after using the Comparative Example 1 for 2 weeks, while the skin elasticity was improved by 6.6% after using the Example 1, which is remarkably excellent.

&Lt; Test Example 3 > In vitro test of moisture resistance

Fig. 3 shows the shape of the artificial dermis immediately after application and after 5 hours of application, to the dermal equivalent made of collagen and fibroblasts, respectively, and Example 1 and Comparative Example 1, The results are shown in Fig.

3 and 4, it can be seen that the collagen gel shape is disturbed by moisture evaporation in Comparative Example 1, whereas Example 1 inhibits moisture evaporation and maintains the original shape of the collagen gel.

Further, it can be confirmed that the moisture evaporation amounts of Comparative Example 1 and Example 1 differ by more than 10% after 5 hours.

[Test Example 4] Expression test of tight junction protein In vitro test

Tight Junction plays an important role in maintaining and strengthening the barrier function of the skin. The function of this tight junction can be confirmed by occludin, a cell membrane protein specifically expressed in tight junctions. When the expression of tight junction protein is inhibited, the transepidermal water loss (TEWL) And the relationship between the two.

Human-derived keratinocytes (HaCaT) were cultured on cover slips for microscopic observation. At this time, the groups treated with Comparative Example 1 and Example 1 were separately cultured. After 24 hours, 1mM CaCl _2, 1mM MgCl _2, washed with PBS and fixed including the cells with paraformaldehyde solution. The permeability of the cell membrane was increased with 0.1% Triton X-100 solution, and the primary antibody against the occludin was stuck for 1 hour. After washing the cells again with PBS, a secondary antibody (Alexar fluor 488) was stuck for 1 hour for fluorescence observation. Fluorescence microscopy (LSM510) was used to observe changes in the amount of occludin protein expression. The results are shown in Fig.

As a result, it was impossible to observe the expression of a tight junction specific protein, ecludine, in human-derived keratinocytes treated with Comparative Example 1, but the expression of ecludin increased in human-derived keratinocytes treated with Example 1 .

Claims (4)

A cosmetic composition for moisturizing the skin, comprising an extract of sea tangle, an extract of sea mustard and a tea extract as an active ingredient. The cosmetic composition for skin moisturizing according to claim 1, wherein the sea tangle extract is seaweed extract, and the sea mustard extract is a sea mustard extract. The cosmetic composition according to claim 1, wherein the sea tangle extract, wakame extract, and tea extract are contained in an amount of 0.001 to 10 wt% based on the total weight of the composition. A cosmetic composition for enhancing skin elasticity comprising an active ingredient of sea tangle extract, wakame extract, and tea extract.

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