KR102280941B1 - Skin moisturizing and skin soothing cosmetic composition comprising Aloe barbadensis leaf juice and extracts of Pulsatilla koreana, Centella asiatica, Portulaca oleracea and Hamamelis virginiana - Google Patents

Abstract

The present invention provides a gel-type skin moisturizing or moisturizing agent comprising Pulsatilla koreana extract, Centella asiatica extract, Portulaca oleracea extract, witch hazel extract and Aloe barbadensis Leaf Juice It relates to a cosmetic composition for skin soothing, and the cosmetic composition according to the present invention contains a walnut flower extract, centella asiatica extract, purslane extract, witch hazel extract and aloe vera leaf juice in a specific composition ratio, thereby significantly improving skin moisturizing and skin soothing effects. It works.

Description

Skin moisturizing and skin soothing cosmetic composition comprising Aloe barbadensis leaf juice and extracts of Pulsatilla koreana, Centella asiatica, Portulaca oleracea and Hamamelis virginiana}

The present invention relates to a gel-type skin moisturizing or moisturizing agent comprising Pulsatilla koreana extract, Centella asiatica extract, Portulaca oleracea extract, witch hazel ( Hamamelis virginiana ) extract and Aloe barbadensis Leaf Juice. It relates to a cosmetic composition for soothing the skin.

In the human body, the skin is an organ that functions as a protective film that protects the living body from the external environment. It prevents the loss of biological components, such as water or electrolyte, and at the same time prevents harmful substances from entering the human body . Such skin is largely divided into an epidermal layer, a dermal layer, and a subcutaneous fat layer. The epidermis layer is composed of keratinocytes and melanocytes. Because the outermost layer of keratinocytes is in direct contact with the external environment, strong resistance to physical, chemical or substance permeation is required, and it prevents moisture loss from the human body to the outside. At the same time as giving, it should maintain its flexibility by retaining adequate moisture itself.

In general, the moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer and becomes about 10 to 30% in the keratinocyte layer. The water supplied from the dermal layer mainly moves to the upper part of the stratum corneum by passive diffusion and is finally discharged to the outside. This is called Trans Epidermal Water Loss (TEWL), and it is known that it is the sebum component and epidermal lipid, which are components of the lipid membrane of the keratinocyte layer, that maintain this transdermal water loss at an appropriate level. In addition, it is known that a substance with hydrophilic water retention capacity called Natural Moisturizing Factor (NMF) is present in the keratinocyte layer and plays an important role in moisturizing the skin. When the moisture in the normal keratinocyte layer is maintained at about 10 to 30%, the skin is smooth and soft, and the body's protective function is normally exhibited. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the protective function of the body is lost, and aging occurs. For example, in the case of dry skin, the cohesion of keratinocytes is weakened, resulting in a scaling phenomenon in which keratinocytes are peeled off like scales from the skin surface. This is because it is smaller than normal skin. In addition, even with healthy skin, the skin condition may deteriorate due to a lack of moisture due to a harsh external environment, that is, wind, cold weather, sunlight, face washing, shaving, etc. Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer of the skin. It is known that when the moisture in the stratum corneum is 10% or less, the skin becomes dry and causes skin problems. The amount of water in the stratum corneum produced by regular keratinization is always maintained at 10-20%.

Currently, an occlusive agent is used to retain moisture in the skin, and the occlusive agent is a hydrophobic substance that promotes moisture retention by forming a barrier on the skin that prevents moisture loss. The most commonly used occlusive agents include vaseline, lanolin, cocoa butter, mineral oil and silicone. In addition, 20 to 50 kinds of various raw materials are generally used to make cosmetics, such as Sodium Lauryl Sulfate, Dimethyl Sulfoxide, Croton Oil, Sodium Hydroxide, etc. When these raw materials are applied to the product, they can cause irritation, such as inflammation, itching, and allergies to the skin.

Under this background, the present inventors have continued their research to develop a material that exhibits excellent skin moisturizing effect while having few side effects such as skin irritation and having a skin soothing effect on skin troubles and skin damage. The present invention was completed by discovering a combination of natural extracts having an effect at the same time and an optimal blending ratio thereof.

Korean Patent No. 10-1082644

An object of the present invention is to provide a cosmetic composition for moisturizing or soothing the skin.

In order to achieve the above object,

The present invention is for moisturizing or soothing the skin, including Pulsatilla koreana extract, Centella asiatica extract, Portulaca oleracea extract, witch hazel ( Hamamelis virginiana ) extract, and Aloe barbadensis Leaf Juice A cosmetic composition is provided.

In addition, the present invention is to prepare a mixture of Halmi flower ( Pulsatilla koreana ) extract, Centella asiatica extract, purslane ( Portulaca oleracea ) extract, witch hazel ( Hamamelis virginiana ) extract and aloe vera leaf juice ( Aloe barbadensis Leaf Juice) step (step 1); Preparing a primary aged product in which the mixture of step 1 is first aged at 10-25° C. for 2-10 hours (step 2); and preparing the secondary aged product in which the primary aged product of step 2 is secondarily aged at 1-9°C for 4-24 hours (step 3); Provided is a cosmetic composition for soothing.

The cosmetic composition for moisturizing or soothing the skin according to the present invention contains a walnut flower extract, centella asiatica extract, purslane extract, witch hazel extract and aloe vera leaf juice in a specific composition ratio, so that the skin moisturizing and skin soothing effect is significantly improved. It works.

The present inventors are for moisturizing or soothing the skin, including Pulsatilla koreana extract, Centella asiatica extract, purslane ( Portulaca oleracea ) extract, witch hazel ( Hamamelis virginiana ) extract and Aloe barbadensis Leaf Juice The present invention was completed by discovering that a cosmetic composition containing the cosmetic composition exhibits a skin moisturizing effect and a skin soothing effect.

In particular, in the present invention, a cosmetic obtained by mixing Pulsatilla koreana extract, Centella asiatica extract, Portulaca oleracea extract, witch hazel ( Hamamelis virginiana ) extract and Aloe barbadensis Leaf Juice in a specific composition ratio. When the composition is used, it has been experimentally proven that the whitening effect is significantly improved compared to the composition including some of these components or the composition including other composition ratios (see Experimental Examples 1 and 2).

Hereinafter, the present invention will be described in detail.

Cosmetic composition for skin moisturizing or whitening

The present invention is for moisturizing or soothing the skin, including Pulsatilla koreana extract, Centella asiatica extract, Portulaca oleracea extract, witch hazel ( Hamamelis virginiana ) extract, and Aloe barbadensis Leaf Juice A cosmetic composition is provided.

In the present invention, 'extract' means that the extract is separated and obtained from the active ingredient raw material using a method known in the art for extraction and separation, and the extract defined in the present invention is prepared by using an appropriate solvent in the above method. It may be extracted by pulverizing or as it is the active ingredient raw material prepared as In this case, as a suitable solvent that can be used to obtain the extract, any solvent acceptable in the art may be used, and water or an organic solvent may be used. For example, purified water, methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol), alcohols having 1 to 4 carbon atoms including butanol (butanol), acetone (acetone), ether (ether) , benzene (benzene), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride), hexane (hexane) and various solvents such as cyclohexane (cyclohexane) can be used alone or in combination, but this Not limited.

Preferably, the extract included in the composition of the present invention uses at least one extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, hydrocarbon-based solvents, or mixed solvents thereof, respectively. may be extracted, and more preferably, may be extracted using one or more extraction solvents selected from water or ethanol.

As the extraction method, any one of methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method, compression method, etc. can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process, and may be purified using a conventional purification method. There is no limitation on the method for preparing the extract of the present invention, and any known method may be used.

For example, the extract included in the composition of the present invention may be used as it is while storing the primary extract extracted by the solvent extraction method described above, centrifugation, filtration, concentration, freeze-drying and storing in a refrigerator, It may be prepared in a powder state by an additional process such as distillation and freeze drying or spray drying. In addition, the primary extract is further purified using various chromatography methods such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, and the like. can also get

Therefore, in the present invention, the extract is a concept including all extracts, fractions, and purified products obtained in each step of extraction, fractionation or purification, and dilutions, concentrates or dried products thereof.

In the cosmetic composition for moisturizing or soothing the skin according to the present invention, the cosmetic composition comprises 0.005-0.055 parts by weight of the purslane extract, 0.005-0.055 parts by weight of the purslane extract, 0.050-0.150 parts by weight of the Centella asiatica extract, 0.010-0.050 parts by weight of the aloe vera leaf juice. and 0.050-0.150 parts by weight of the witch hazel extract, preferably 0.010-0.050 parts by weight of the purslane extract, 0.010-0.050 parts by weight of the purslane extract, 0.060-0.140 parts by weight of the Centella asiatica extract, 0.015-0.045 parts by weight of the aloe vera leaf juice and 0.060-0.140 parts by weight of the witch hazel extract, and more preferably 0.020-0.040 parts by weight of the purslane extract 0.020-0.040 parts by weight, 0.080-0.120 parts by weight of Centella asiatica extract, 0.020-0.040 parts by weight of aloe vera leaf juice, based on 0.010 parts by weight of the purslane extract. parts and 0.080-0.120 parts by weight of the witch hazel extract, 0.025-0.035 parts by weight of the purslane extract, 0.025-0.035 parts by weight of the purslane extract, 0.090-0.110 parts by weight of the Centella asiatica extract, 0.025-0.035 parts by weight of the aloe vera leaf juice, and the witch hazel It is particularly preferable to include 0.090-0.110 parts by weight of the extract.

The cosmetic composition according to the present invention is a gel, serum, lotion, essence, paste, mask pack, patch, cream, lotion, nourishing lotion, nourishing cream, moisture cream, massage cream, powder, soap, cleanser, oil, foundation, makeup base , may be one or more formulations selected from the group consisting of wax and spray, and may be preferably used as a gel formulation.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. It is in any formulation suitable for topical application, for example solutions, gels, solid or kneaded dry products, emulsions obtained by dispersing the oily phase in an aqueous phase, suspension microemulsions, microcapsules, microgranules or ionic (liposomes) and/or bi It may be provided in the form of a warm vesicular dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition of the present invention can be prepared in the form of an adjuvant that can be applied topically or systemically commonly used in the field of dermatology by containing a dermatologically acceptable medium or base, and these compositions are conventional in the art. It can be prepared according to the method.

In the cosmetic composition of the present invention, in the composition for external application of each formulation, components other than the above-mentioned essential components can be appropriately selected and formulated by those skilled in the art without difficulty depending on other formulations or purposes of use.

In addition, the cosmetic composition of the present invention may further include a skin conditioning agent that can help the skin, such as a skin moisturizer, a skin softener, and a moisture barrier, in addition to the active ingredients, and a fatty substance, an organic solvent, a solubilizer, and a concentration. Agents and gelling agents, emollients, emollients, antioxidants, suspending agents, stabilizers, pH adjusters, foaming agents, fragrances, surfactants, water, emulsifiers, thickeners, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents , wetting agents, essential oils, fragrances, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics. These adjuvants are introduced in amounts generally used in the field of cosmetology.

According to an embodiment of the present invention, the cosmetic composition for moisturizing or soothing the skin according to the present invention comprises panthenol, sodium hyaluronate, green tea extract, licorice leaf extract and allantoin as ingredients exhibiting skin soothing or trouble improving activity, Glycerin and urea as a moisturizer, butylene glycol as a skin conditioning ingredient for skin improvement, rose flower water, coconut extract, oleander leaf extract, chrysanthemum fruit extract, earthenia extract, beta-glucan and chamomile flower extract, other ingredients Further comprising at least one component selected from the group consisting of carbomer, pentylene glycol, 1,2-hexanediol, potassium hydroxide, sclerotium gum, disodium EDTA and phenoxyethanol as can do. As the solvent, one or more components selected from the group consisting of purified water, dipropylene glycol, propanediol and ethylhexanediol may be used. At this time, based on 0.010 parts by weight of the purslane extract, 0.300 to 0.400 parts by weight of a component exhibiting the skin soothing or trouble improving activity, 7.150 to 7.250 parts by weight of a moisturizer, 12.680 to 12.780 parts by weight of a skin conditioning component, 0.880 to 0.980 parts by weight of other components , solvent 78.000 to 79.000 parts by weight, preferably 0.330 to 0.350 parts by weight of a component exhibiting skin soothing or trouble improving activity, 7.195 to 7.215 parts by weight of a moisturizer, 12.715 to 12.735 parts by weight of a skin conditioning component, 0.920 other components to 0.940 parts by weight, and 78.520 to 78.540 parts by weight of a solvent may be included, but is not limited thereto.

In addition, the present invention is to prepare a mixture of Halmi flower ( Pulsatilla koreana ) extract, Centella asiatica extract, purslane ( Portulaca oleracea ) extract, witch hazel ( Hamamelis virginiana ) extract and aloe vera leaf juice ( Aloe barbadensis Leaf Juice) step (step 1);

Preparing a primary aged product in which the mixture of step 1 is first aged at 10-25° C. for 2-10 hours (step 2); and

preparing a secondary aged product in which the primary aged product of step 2 is secondarily aged at 1-9°C for 4-24 hours (step 3);

It provides a cosmetic composition for skin whitening, characterized in that it is prepared by including.

Preferably, the primary aging of step 2 is to be aged for 4-8 hours at 13-21°C, and the secondary aging of step 3 may be aging at 3-7°C for 10-20 hours, more More preferably, the primary aging of step 2 may be aging at 16-18° C. for 5-7 hours, and the secondary aging of step 3 may be aging at 4-6° C. for 14-16 hours.

The mixture of step 1 is a mixture of 0.005-0.055 parts by weight of the purslane extract, 0.050-0.150 parts by weight of Centella asiatica extract, 0.010-0.050 parts by weight of aloe vera leaf juice, and 0.050-0.150 parts by weight of witch hazel extract based on 0.010 parts by weight of the purslane extract. Preferably, based on 0.010 parts by weight of the purslane extract, 0.010-0.050 parts by weight of the extract of balsamina flower, 0.060-0.140 parts by weight of the Centella asiatica extract, 0.015-0.045 parts by weight of aloe vera leaf juice, and 0.060-0.140 parts by weight of the witch hazel extract. And more preferably, based on 0.010 parts by weight of the purslane extract, 0.020 to 0.040 parts by weight of the extract, 0.080-0.120 parts by weight of Centella asiatica extract, 0.020-0.040 parts by weight of aloe vera leaf juice, and 0.080-0.120 parts by weight of the witch hazel extract are mixed It is particularly preferable to mix 0.025-0.035 parts by weight of Haltifolia extract, 0.090-0.110 parts by weight of Centella asiatica extract, 0.025-0.035 parts by weight of aloe vera leaf juice and 0.090-0.110 parts by weight of witch hazel extract based on 0.010 parts by weight of purslane extract.

Hereinafter, the present invention will be described in more detail by way of Examples. These examples are merely for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited to these examples.

< manufacturing example 1> Halmi flower, Centella asiatica , purslane and witch hazel Preparation of extracts

Halmi flower ( Pulsatilla k oreana ) 100 g of freeze-dried powder was added with 1 L of purified water as an extraction solvent and extracted at 70 ° C. for 6 to 12 hours in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) to obtain an extract. The extract was filtered with a mesh filter (stainless steel filter mesh, R&K, China, 4-400 mesh). The filtered filtrate was concentrated under reduced pressure using a rotary evaporator (DL10-3000, Greatwall, 20L) to remove the solvent, thereby preparing an extract of Haltifolia flower. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

In addition, using the same method as above, centella leaves ( Centella aiatica Leaf) freeze-dried powder, purslane flower, leaf and stem ( Portulaca o leracea Flower/Leaf/Stem) freeze-dried powder and witch hazel leaf ( Hamamelis v irginiana Leaf) freeze-dried powder, centella asiatica extract, purslane extract and witch hazel, respectively An extract was prepared.

< manufacturing example 2> Aloe vera leaf juice Preparations

As a juice obtained by pressing Aloe vera leaves, commercially available Aloe barbadensis Leaf Juice was purchased and used.

< manufacturing example 3> Aloe Vera Leaf Juice and preparation of a complex of four extracts

Prepare a mixture by dissolving the Aloe vera leaf juice of Preparation Example 2 and the extract prepared by the method of Preparation Example 1, Centella asiatica extract, purslane extract, and witch hazel extract in purified water, and the mixture was heated at 10 to 25° C. for 4 to 12 It was allowed to stand for a period of time to obtain a primary aged product, and then filtered through a mesh filter (stainless steel filter mesh, R&K, China, 4-400mesh). The filtrate obtained by filtering the first aged product was left at 1 to 5° C. for 4 to 24 hours and then secondary aged to obtain a secondary aged product, which was obtained using a mesh filter (stainless steel filter mesh, R&K, China, 4~ 400 mesh), the solvent was removed using a rotary evaporator (DL10-3000, Greatwall, 20L) to prepare a complex of aloe vera leaf juice and four extracts. The complex was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

< Example and comparative example > Aloe Vera Leaf Juice and a complex of four extracts. cosmetics Preparation of the composition

A cosmetic composition in the form of a gel containing a complex of aloe vera leaf juice and four extracts was prepared according to the composition shown in Table 1 below.

At this time, the complex or individual extracts obtained by mixing Aloe vera leaf juice and the extract of genus oleifera, Centella asiatica, purslane extract and witch hazel extract of Preparation Example 1 in the composition shown in Table 1 below were mixed at 17° C. using the same method as in Preparation Example 3. It was prepared by primary aging for 8 hours and secondary aging at 3°C for 15 hours.

At this time, the content of the remaining components was expressed in parts by weight based on parts by weight of the following purified water (73.08 parts by weight, see Table 2).

(unit:
parts by weight) pasqueflower
extract Centella asiatica
extract purslane
extract Aloe Vera Leaf Juice witch hazel
extract Example 1-1 0.01 0.10 0.01 0.03 0.10 Example 1-2 0.02 0.10 0.01 0.03 0.10 Examples 1-3 0.03 0.10 0.01 0.03 0.10 Examples 1-4 0.04 0.10 0.01 0.03 0.10 Examples 1-5 0.05 0.10 0.01 0.03 0.10 Example 2-1 0.03 0.06 0.01 0.03 0.10 Example 2-2 0.03 0.08 0.01 0.03 0.10 Example 2-3 0.03 0.10 0.01 0.03 0.10 Example 2-4 0.03 0.12 0.01 0.03 0.10 Example 2-5 0.03 0.14 0.01 0.03 0.10 Comparative Example 1 0.03 0.10 - - - Comparative Example 2 0.03 - 0.01 0.03 0.10 Comparative Example 3 - 0.10 0.01 0.03 0.10 Comparative Example 4 0.03 - - - - Comparative Example 5 - 0.10 - - - Comparative Example 6 - - 0.01 0.03 0.10

In addition to the active ingredients in Table 1, other ingredients added to the cosmetic composition are as shown in Table 2 below.

other ingredients parts by weight Soothes skin and improves troubles Panthenol, Sodium Hyaluronate, Green Tea Extract, Licorice Leaf Extract, Allantoin 0.339 moisturizer glycerin, urea 7.205 skin conditioning Butylene Glycol, Rose Flower Water, Coconut Extract, Old Jujube Tree Leaf Extract, Chopi Tree Fruit Extract, Earthenia Extract, Beta-Glucan, Chamomile Flower Extract 12.726 Etc Carbomer, pentylene glycol, 1,2-hexanediol, potassium hydroxide, sclerotium gum, disodium EDTA, phenoxyethanol 0.931 Solvent 1 Purified water 73.080 Solvent 2 Dipropylene glycol, propanediol, ethyl hexanediol 5.449

< Experimental example 1> Water retention evaluation

The water retention ability of the cosmetic compositions prepared in Examples 1-1 to 2-5 and Comparative Examples 1 to 6 was measured.

Specifically, each of the cosmetic compositions was added to a Petri dish, wetted with paper of an appropriate size, and then the weight of the paper was weighed. Each paper containing the cosmetic composition was placed in an empty Petri dish and the weight of the paper was measured over time to confirm the water retention ability. Purified water was used as a negative control.

Water retention capacity was evaluated using the following formula.

Moisture retention capacity (%) = (weight after elapsed time - weight of paper) × 100/(initial weight - weight of paper)

Water retention capacity (%) 0 minutes 30 minutes 60 minutes 90 minutes 120 minutes 150 minutes 180 minutes Example 1-1 100 72.4 64.1 52.2 48.4 37.1 32.9 Example 1-2 100 93.7 89.4 73.4 68.9 61.4 55.3 Examples 1-3 100 100 94.1 83.5 79.8 70.8 64.2 Examples 1-4 100 94.4 88.6 72.5 67.6 60.7 54.9 Examples 1-5 100 73.2 63.8 51.4 44.6 38.4 31.6 Example 2-1 100 71.0 62.7 55.1 50.9 39.8 34.1 Example 2-2 100 92.6 87.6 72.1 66.7 60.6 55.1 Example 2-3 100 100 94.1 83.5 79.8 70.8 64.2 Example 2-4 100 95.8 88.2 73.2 67.9 62.4 56.5 Example 2-5 100 72.1 64.9 52.6 48.6 35.6 31.4 Comparative Example 1 100 59.6 47.8 42.7 30.1 25.0 20.8 Comparative Example 2 100 69.2 59.8 49.5 42.3 29.9 25.4 Comparative Example 3 100 67.1 56.5 48.3 41.7 28.5 24.7 Comparative Example 4 100 52.4 42.3 35.4 22.4 19.9 17.2 Comparative Example 5 100 51.7 44.2 31.1 21.2 19.4 16.0 Comparative Example 6 100 55.8 41.5 38.6 26.4 21.5 18.9 Con (purified water) 100 0 0 0 0 0 0

As shown in Table 3 above, the cosmetic compositions of Examples 1-1 to 2-5, including a complex of an extract of walrus flower, centella asiatica, purslane extract, aloe vera leaf juice and witch hazel extract according to the present invention, were prepared for the same time It was confirmed that the water retention ability was superior to those of Comparative Examples 1 to 6 including some combinations. In particular, the cosmetic compositions prepared in Examples 1-3 and 2-3 showed the best water retention ability.

<Experimental Example 2> Measurement of skin barrier function recovery effect

In order to measure the effect on the recovery of the skin barrier function damaged due to skin damage, 20 μl of SLS 10% aqueous solution was applied to the fin-chamber of 30 adult male and female upper arms and applied for 16 hours to damage the skin barrier. While applying the cosmetic compositions of 1-1 to 2-5 and Comparative Examples 1 to 6, transdermal water loss (TEWL) was measured once a day for 7 days, and the results are shown in Table 4 below.

Change in transdermal water loss (TEWL) (%) before treatment 1 day 2 days 3 days 4 days 5 days Example 1-1 100 96 89 77 54 41 Example 1-2 100 93 79 60 38 22 Examples 1-3 100 91 75 52 31 17 Examples 1-4 100 92 80 58 37 21 Examples 1-5 100 95 88 78 55 42 Example 2-1 100 96 89 78 54 42 Example 2-2 100 92 80 59 38 22 Example 2-3 100 91 75 53 30 17 Example 2-4 100 93 78 57 36 20 Example 2-5 100 94 87 76 53 40 Comparative Example 1 100 100 93 83 60 49 Comparative Example 2 100 97 91 80 58 46 Comparative Example 3 100 98 91 82 59 48 Comparative Example 4 100 100 97 88 63 54 Comparative Example 5 100 103 96 86 64 52 Comparative Example 6 100 99 95 84 62 51

As shown in Table 5, when the cosmetic compositions of Examples 1-1 to 2-5 were treated compared to Comparative Examples 1 to 6, the amount of transdermal water loss returned to normal at a rapid rate, and the barrier damage was recovered. In particular, it was confirmed that the cosmetic compositions of Examples 1-3 and 2-3 had the best effect of restoring the skin barrier function.

< Experimental example 3> cosmetics skin soothing and Skin trouble Palliative efficacy analysis

The skin soothing and skin trouble alleviation effects of the cosmetic compositions prepared in Examples 1-1 to 2-5 and Comparative Examples 1 to 6 were confirmed.

Specifically, after dividing the volunteers with severe skin troubles so that there are 5 people per evaluation group, the essence prepared in Examples 1-1 to 3-5 and Comparative Examples 1 to 6 corresponding to each group is provided 4 The product was used at least once a day for a week. The skin condition before and 4 weeks after use was evaluated, and the average was calculated to evaluate the skin trouble alleviation effect and skin soothing effect of each test substance.

A scale of 0 to 10 was used as the evaluation criteria, and skin conditions such as dryness, itching, and stinging were indicated from 10 points to 'very sensitive' and 0 points for 'very calming'. The experimental results are shown in Table 5 below as the average score of the five evaluation subjects.

immediately after after 4 weeks Example 1-1 7.5 3.4 Example 1-2 7.6 1.0 Examples 1-3 7.8 0.4 Examples 1-4 7.5 1.2 Examples 1-5 7.8 3.3 Example 2-1 7.7 3.5 Example 2-2 7.6 1.3 Example 2-3 7.8 0.4 Example 2-4 7.7 1.1 Example 2-5 7.8 3.2 Comparative Example 1 7.6 4.3 Comparative Example 2 7.9 4.2 Comparative Example 3 7.8 4.1 Comparative Example 4 7.7 5.5 Comparative Example 5 7.8 5.6 Comparative Example 6 7.9 5.6

As shown in Table 5 above, the cosmetic composition of Comparative Examples 1 to 6 comprising only a single or a combination of a prickly pear flower extract, centella asiatica extract, purslane extract, aloe vera leaf juice and witch hazel extract was used, compared to the case of using the cosmeceae extract, centella asiatica extract. , purslane extract, aloe vera leaf juice, and witch hazel extract, when the cosmetic composition of Examples 1-1 to 2-5 was used, skin conditions such as dryness, itching, and stinging were more calmed. In particular, when the cosmetic compositions of Examples 1-3 and 2-3 were applied, it was confirmed that the skin soothing effect was the most excellent.

<Experimental Example 4> Confirmation of skin moisturizing effect

In order to examine the skin moisturizing effect of the cosmetic compositions of Examples 1-1 to 2-5 and Comparative Examples 1 to 3, an experiment was conducted as follows. Thirty-nine people in their 20s and 40s without skin disease were selected and divided into 3 × 3 cm in size using an oil-based pen on the forearm and applied twice daily for 4 weeks. At this time, before the start of application, the skin conductivity was measured using a Corneometer (CM820 Corage Khazaka Electronic GmbH, Germany) under constant temperature and humidity conditions (24 degrees, 40% humidity) in advance, taking the default value (100%), 1 week, The increase rate was evaluated by measuring the skin conductivity after 2 weeks and 4 weeks. The results are shown in Table 6 below.

Skin conductivity increase rate (%) before application 1 week later after 2 weeks after 4 weeks Example 1-1 100 108 111 120 Example 1-2 100 112 119 136 Examples 1-3 100 116 123 142 Examples 1-4 100 114 120 139 Examples 1-5 100 107 113 122 Example 2-1 100 106 110 121 Example 2-2 100 113 120 137 Example 2-3 100 116 123 142 Example 2-4 100 115 121 138 Example 2-5 100 108 112 123 Comparative Example 1 100 103 107 113 Comparative Example 2 100 106 109 116 Comparative Example 3 100 105 107 117

As shown in Table 6 above, in the case of Examples 1-1 to 2-5, which is a cosmetic composition comprising all of the extract of Haltifolia flower, Centella asiatica, purslane extract, aloe vera leaf juice and witch hazel extract of the present invention, Comparative Example containing only some combinations It was confirmed that the skin conductivity increase rate was excellent compared to those of 1 to 3. Since the skin conductivity is proportional to the amount of moisture in the skin, it can be seen that the cosmetic composition comprising the extract of the present invention, the extract of walrus flower, centella asiatica, purslane extract, aloe vera leaf juice and witch hazel extract has excellent skin moisturizing effect. The cosmetic compositions of Examples 1-3 and 2-4 showed the most excellent skin moisturizing effect.

< Experimental example 5> Effect evaluation of skin soothing, erythema relief and skin moisturizing improvement according to aging conditions

The skin soothing, erythema relieving efficacy, and skin moisturizing effect of the complex extract prepared under different aging conditions of Preparation Example 2 were evaluated.

Specifically, it was prepared in the same manner as in Example 1-3 (=2-3), except that 0.03 parts by weight of aloe vera leaf juice of Preparation Example 2 and 0.03 parts by weight of the genus oleifera extract of Preparation Example 1, 0.1 parts by weight of Centella asiatica extract, purslane A mixture of 0.03 parts by weight of extract and 0.1 parts by weight of witch hazel extract was subjected to primary and secondary aging under the conditions of temperature (°C) and time (h) in Table 5 below to prepare a composite, the composite and Table 2 The cosmetic compositions of Examples 3-1 to 3-23 were prepared by mixing the other ingredients of

For men and women in their 20s and 30s who do not currently have skin allergy symptoms, the group was divided to be 10 per each evaluation group, and then skin irritation was induced through the Tape Stripping Method on the inner part of the lower arm, and then the The degree to which irritation induced by applying the essence prepared in Examples 1-1 to 3-5 and Comparative Examples 1 to 6 was calmed was relative evaluated. First, skin erythema was induced by taping 30 times with general scotch tape on two inner parts of the lower arm. After erythema was induced, the essence of Examples 1-1 to 3-5 and Comparative Examples 1 to 6 according to the present invention was applied, respectively. The degree of skin soothing was evaluated 3 hours after application.

A scale of 0 to 5 was used as the evaluation criteria, with 0 points for 'no response', 1 point for 'very slight erythema', 2 points for 'dim or mild erythema', and 3 points for 'demarcated but weak erythema'. Dots and 'clear erythema' were scored as 4 points, and 'severe erythema' as 5 points. The experimental results are shown in Table 3 below as the average score of 10 subjects to be evaluated.

The skin moisturizing power improvement effect was measured in the same manner as in Experimental Example 4, and the increase in skin conductivity (%) before and 4 weeks after application is shown in Table 7 below.

1st ripening condition Second ripening conditions Degree of skin irritation (erythema) Skin conductivity increase rate (%) temperature time temperature time left right before application after 4 weeks Example 4-1 17 8 One 15 1.8 2.0 100 122 Example 4-2 17 8 2 15 0.9 0.8 100 137 Example 4-3 17 8 3 15 0.1 0.1 100 142 Example 4-4 17 8 4 15 0.8 0.9 100 138 Example 4-5 17 8 5 15 1.9 1.8 100 121 Example 4-6 17 8 3 4 2.0 1.9 100 120 Example 4-7 17 8 3 10 0.8 0.9 100 135 Examples 4-8 17 8 3 15 0.1 0.1 100 142 Examples 4-9 17 8 3 20 0.9 0.8 100 139 Example 4-10 17 8 3 24 1.8 2.0 100 122 Examples 4-11 10 8 3 15 2.0 1.9 100 121 Example 4-12 13 8 3 15 0.9 0.8 100 138 Examples 4-13 17 8 3 15 0.1 0.1 100 142 Examples 4-14 21 8 3 15 0.8 0.8 100 137 Examples 4-15 25 8 3 15 1.9 1.9 100 122 Examples 4-16 17 4 3 15 1.9 2.0 100 120 Example 4-17 17 6 3 15 0.8 0.8 100 136 Examples 4-18 17 8 3 15 0.1 0.1 100 142 Examples 4-19 17 10 3 15 0.9 0.8 100 139 Examples 4-20 17 12 3 15 1.8 1.9 100 123 Example 4-21 17 8 not performed not performed 2.3 2.3 100 116 Example 4-22 not performed not performed 3 15 2.3 2.4 100 117 Example 4-23 not performed not performed not performed not performed 2.5 2.5 100 111

As shown in Table 7, when using a cosmetic composition comprising a composite prepared by primary aging at 13 to 21 ° C. for 6 to 10 hours, and then secondary aging at 2 to 4 ° C. for 10 to 20 hours, A cosmetic composition comprising a composite prepared under conditions other than the above, and the cosmetic composition of Examples 4-21 to 4-23 prepared with a composite that was aged only once or did not undergo aging under the conditions of primary aging or secondary aging Skin irritation was further reduced and skin conductivity was further increased In particular, when a cosmetic composition comprising a complex prepared by first aging at 17° C. for 8 hours and then secondary aging at 3° C. for 15 hours is applied, the effect of soothing the erythema-induced skin and improving skin moisturizing power was found to be the best.

So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (6)

delete delete delete delete Purslane ( Portulaca Oleracea ) extract 0.01 parts by weight, Pulsatilla koreana extract 0.0285-0.0315 parts by weight, Centella Asiatica extract 0.095-0.105 parts by weight, witch hazel ( Hamamelis Virginiana ) extract 0.095-0.105 parts by weight and aloe vera Preparing a mixture of 0.0285-0.0315 parts by weight of Aloe Barbadensis Leaf Juice (step 1);
Preparing a primary aged product in which the mixture of step 1 was first aged at 16.5-17.5° C. for 7.5-8.5 hours (step 2); and
preparing a secondary aged product in which the primary aged product of step 2 is secondarily aged at 2.5-3.5° C. for 14.5-15.5 hours (step 3);
A cosmetic composition for skin moisturizing or skin soothing, characterized in that it is prepared by including.
6. The method of claim 5,
The cosmetic composition is a gel, serum, lotion, essence, paste, mask pack, patch, cream, lotion, nourishing lotion, nourishing cream, moisture cream, massage cream, powder, soap, cleanser, oil, foundation, makeup base, wax and A cosmetic composition, characterized in that it is one selected from the group consisting of a spray.