KR102058294B1 - Cosmetic composition for skin regenerating containing complex extracts of snail mucus, Boswellia serrata resin and Aloe barbadensis leaf, and cream comprising the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin regeneration containing a snail mucus filtrate, a Boswellia serrata resin extract, and an aloe barbadensis leaf extract; and cream including the same. According to the present invention, the cosmetic composition for skin regeneration contains the snail mucus filtrate, the Boswellia serrata resin extract, and the aloe barbadensis leaf extract by a predetermined composition ratio, thereby providing an effect of remarkably increasing skin regeneration effect.

Description

Cosmetic composition for skin regenerating containing complex extracts of snail mucus, Boswellia serrata resin and Aloe barbadensis leaf, and cream containing the same }

The present invention is a snail mucus filtrate (snail mucus filtrate), Indian frankincense resin ( Boswellia serrata The present invention relates to a cosmetic composition for skin regeneration containing a resin extract and an Aloe barbadensis leaf extract and a cream comprising the same.

The skin is located at the outermost part of the human body and is in direct contact with the external environment, protecting the human body from external damage and preventing excessive evaporation of moisture and heat in the body. The skin is also composed of three layers and is divided into the epidermis, dermis and subcutaneous tissue from the outermost. The epidermis is composed of stratum basale, stratum spinosum, stratum granulosum, and stratum corneum. Finally, differentiated dead cells accumulate on the surface of the skin to function as a skin barrier. Most of the epidermis is composed of keratinocytes. These keratinocytes form keratin through proliferation, migration and differentiation from the basal layer to protect the skin, and produce various cytokines to participate in inflammatory and immune responses. In addition, it plays a role in the growth of surrounding cells, and in particular, the proliferation of keratinocytes is known to have an epidermal regeneration effect.

As the skin of the human body ages, the proliferation of keratinocytes or fibroblasts decreases, and the epidermal and dermal borders are flattened, and even minor skins are easily peeled off or the ability of skin regeneration and wound healing begins to decline.

Accordingly, researches on natural materials with excellent effects of inducing proliferation and migration of keratinocytes and fibroblasts having reduced physiological activity due to skin aging and skin lesions while alleviating side effects of synthetic materials have been actively conducted. Through natural substances with efficacy, it can exhibit effects on skin regeneration and wound healing prevention as well as skin aging prevention.

On the other hand, about 20,000 species of snail are meat shellfish belonging to the gastropod. Although the mobility is significantly lower than that of other living organisms, it is distributed evenly throughout Korea. Mucin is composed of mucin and mucin is composed of chondroitin sulfate (chondroitin sulfate). Allantoin, which is the main substance of snail mucus, has a soothing and pore contracting effect, and glycolic acid is known to have a skin trouble prevention effect.

Indian frankincense resin ( Boswellia) serrata resin) is a resin extract obtained from Indian frankincense. Indian frankincense ( Boswellia serrata ) is a tree of Boswellia , such as frankincense, which is a large, branched deciduous tree that grows abundantly in dry hills in India. This plant's resinous exudate is an external anti-inflammatory agent that cures various inflammatory symptoms in India, and has been used in folk medicine for indigenous people.

Aloe vera (also known as Aloe barbadensis ) is a perennial herbaceous perennial herb that is used worldwide as a medicinal plant, but is similar to a cactus in appearance but is actually a lily family or aloe genus. aloe genus), about a tenth of the family Liliaceae. The main components of aloe are macromolecule polysaccharides including hexose, aloetin, aloeolcin, etc., and most of these components are present in the gel inside the leaves. Aloe is widely known as a plant that moisturizes the skin, and is known to form a moisture barrier due to its excellent skin moisturizing effect.

However, the skin regeneration effect of the composition including all of the snail mucus filtrate, the incense frankincense resin and the aloe vera leaf is not known yet. Accordingly, the present inventors have completed the present invention by confirming that the snail mucus filtrate, the Indian frankincense resin extract, and the aloe vera leaf extract are used in combination with a specific composition ratio, which significantly improves skin regeneration efficacy.

Republic of Korea Patent Publication No. 10-2014-0109221

An object of the present invention is to provide a cosmetic composition for skin regeneration.

In order to achieve the above object,

The present invention is a snail mucus filtrate; Indian frankincense resin ( Boswellia serrata) resin) extracts; It provides a cosmetic composition for skin regeneration comprising; and Aloe vera leaf ( Aloe barbadensis leaf) extract.

In addition, the present invention is a snail mucus filtrate (snail mucus filtrate), Indian frankincense resin ( Boswellia serrata preparing a mixture of a resin) extract and an Aloe barbadensis leaf extract (step 1); Preparing a first aged mixture of the mixture of step 1 at 1-5 ° C. for 4-24 hours (step 2); And preparing a secondary aging secondary maturation of the first aging substance of step 2 at 10-25 ° C. for 4-12 hours (step 3). To provide a composition.

The cosmetic composition for skin regeneration according to the present invention contains a snail mucus filtrate, Boswellia serrata resin extract, and Aloe barbadensis leaf extract at a specific composition ratio, thereby improving skin regeneration. This effect is remarkably improved.

Hereinafter, the present invention will be described in detail.

For skin regeneration Cosmetics  Composition

The present invention is a snail mucus filtrate; Indian frankincense resin ( Boswellia serrata) resin) extracts; It provides a cosmetic composition for skin regeneration comprising; and Aloe vera leaf ( Aloe barbadensis leaf) extract.

The snail mucus filtrate according to the present invention may be a filtrate collected by filtering the snail mucus secreted from the snail.

The extract according to the present invention can be used to isolate and obtain the extract using the extraction and separation methods known in the art, the extract defined in the present invention is an active ingredient prepared by the above method using a suitable solvent The raw material can be extracted as it is or by grinding. In this case, any suitable solvent that may be used to obtain the extract may be used as long as it is a solvent acceptable in the art, and water or an organic solvent may be used. For example, alcohol having 1 to 4 carbon atoms, acetone, ether including purified water, methanol, ethanol, propanol, isopropanol, butanol, etc. , Solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. It is not limited.

Preferably, the Indian frankincense resin and the aloe vera leaf extract may each contain one or more extracting solvents selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon solvent, or a mixed solvent thereof. It may be extracted using, and more preferably may be extracted using one or more extraction solvents selected from water or ethanol.

As the extraction method, any one of hot water extraction method, cold leaching extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be used. In addition, the desired extract may further be subjected to a conventional fractionation process, it may be purified using conventional purification methods. There is no limitation on the preparation method of the extract of the present invention, any known method may be used.

For example, the extract contained in the composition of the present invention may be used as it is while the primary extract extracted by the solvent extraction method, centrifuged, filtered, concentrated and freeze-dried and stored in a refrigerator compartment, and the primary extract is decompressed. It may be prepared in powder form by an additional process such as distillation and freeze drying or spray drying. In addition, the primary extract is further purified using a variety of chromatography, such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. You can also get

Therefore, in the present invention, the extract is a concept including all the extracts, fractions and purified products obtained at each step of extraction, fractionation or purification, their dilutions, concentrates or dried products.

In the cosmetic composition for skin regeneration according to the present invention, 45 to 55 parts by weight of snail mucus filtrate, 3-7 parts by weight of aloe vera leaf extract, preferably 1 part by weight of the Indian frankincense resin extract, preferably Snail mucus filtrate based on 1 part by weight of Indian frankincense resin extract may include 49-51 parts by weight, aloe vera leaf extract 4.75-5.25 parts by weight, more preferably snail mucus based on 1 part by weight of Indian frankincense resin extract 49.5-50.5 parts by weight of the filtrate, aloe vera leaf extract may be to include 4.9-5.1 parts by weight.

In addition, the cosmetic composition for skin regeneration according to the present invention, hydrolyzed pea protein, shea butter, castor oil, hydro as a component showing skin protection activity in addition to the snail mucus filtrate, Indian frankincense resin extract and aloe vera leaf extract Gentle caster oil, jojoba seed oil, argan kernel oil and olive oil, hollyhock extract, hydroxyethyl urea and trehalose as components that show moisture moisturizing activity, centella asiatica extract, horticulture root Extract, Golden Extract, Green Tea Extract, Spanish Licorice Root Extract, Matricaria Flower Extract, Rosemary Leaf Extract, Portulaca Extract and Grapefruit Extract, Adenosine as an Anti-Wrinkle Improvement Activity, Capertripeptide-1, Grapefruit Peel Oil , Butylene glycol, Beeswax, Allantoin, Algin , Panthenol and may further additionally comprise one or more components selected from sodium carbonate by hyaluronidase, tocopheryl acetate, and hydroxy rolls rise deugeul lycopene four unexposed glycan group consisting of.

The cosmetic composition according to the present invention is a lotion, nutrition lotion, nutrition cream, moisture cream, massage cream, essence, paste, mask pack, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, makeup base, wax And it may be one or more formulations selected from the group consisting of a spray, it can be preferably used as a cream formulation. Components other than the above essential ingredients may be appropriately selected and blended by those skilled in the art according to other formulations or purposes of use.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspension microemulsions, microcapsules, microgranules or ionic (liposomes) and / or biologies obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a warm vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or concealed stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition of the present invention may be prepared in the form of topically or systemically applicable auxiliaries commonly used in the field of dermatology by containing a dermatologically acceptable medium or base, and these compositions are conventionally used in the art. It may be prepared according to the method.

In addition to the extract of the present invention, the cosmetic composition may contain fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water Commonly used in ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic field, such as any other ingredient. These adjuvants are introduced in amounts commonly used in the cosmetic field.

In addition, the present invention comprises the steps of preparing a mixture of snail mucus filtrate, Boswellia serrata resin extract and Aloe barbadensis leaf extract (step 1); Preparing a first aged mixture of the mixture of step 1 at 1-5 ° C. for 4-24 hours (step 2); And preparing a secondary aging secondary maturation of the first aging substance of step 2 at 10-25 ° C. for 4-12 hours (step 3). To provide a composition.

Preferably the first aging of step 2 is to mature for 10-20 hours at 2-4 ℃, the second aging of step 3 may be to aged for 6-10 hours at 13-21 ℃, even more Preferably, the first ripening step 2 may be aged for 13-17 hours at 2.5-3.5 ° C., and the second ripening step 3 may be aged at 15-19 ° C. for 7-9 hours.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited to these examples.

< Production Example  1> snail mucus filtrate (snail mucus filtrate)  Ready

Snails bred at 25 ° C. temperature and 65% humidity range were washed in purified water and added to the net, and the secreted mucus was collected from the snails for 6 hours. The collected snail mucus is filtered in a filter device having a porous filter medium having a pore size of 250 nanometers (nm) to obtain a snail mucus filtrate, and to prevent deterioration of the snail mucus filtrate at 5 ° C. Stored in a low temperature refrigerator maintained.

< Production Example  2> Indian frankincense resin ( Boswellia serrata Preparation of Resin Extract

50 g of ethanol was added to 20 g of India frankincense resin crushed to 50 mesh or less, and extracted with shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) at 70 ° C. for 6 to 12 hours to obtain an extract. The extract was filtered with a mesh filter (stainless steel filter mesh, R & K, China, 4 ~ 400mesh). The filtered filtrate was removed using a rotary evaporator (DL10-3000, Greatwall, 20L) to remove the solvent, to prepare an Indian frankincense resin extract. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored in a refrigerator at 5 ℃.

< Production Example  3> Aloe vera leaf ( Aloe barbadensis preparation of leaf) extract

50 g of ethanol was added to 20 g of aloe vera leaves pulverized to 50 mesh or less as an extraction solvent, and extracted in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) at 70 ° C. for 6 to 12 hours. The extract was filtered with a mesh filter (stainless steel filter mesh, R & K, China, 4 ~ 400mesh). The filtrate was filtered to remove the solvent using a rotary evaporator (DL10-3000, Greatwall, 20L), and then aloe vera leaf extract was prepared. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored in a refrigerator at 5 ℃.

< Production Example  4> Snail Mucus , Indian frankincense resin , Aloe vera leaf  Preparation of Complex Extracts

Snail mucus filtrate, Indian frankincense resin extract and aloe vera leaf extract prepared by the method of Preparation Examples 1 to 3 in a purified water to prepare a mixture, the mixture was left for 1 to 5 ℃ for 4 to 24 hours 1 After primary aging to obtain a primary aging, it was filtered through a mesh filter (stainless steel filter mesh, R & K, China, 4 ~ 400mesh). The filtrate filtered the primary aging was again left at 10 to 25 ℃ for 4 to 12 hours to give a second aging to obtain a second aging, a mesh filter (stainless steel filter mesh, R & K, China, 4 ~ 400 mesh), and then the solvent was removed using a rotary evaporator (DL10-3000, Greatwall, 20L) to prepare a snail mucus, Indian frankincense resin, aloe vera leaf complex extract. The complex extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5 ℃.

< Example  And Comparative example > Snail Mucus , Indian frankincense resin , Aloe vera leaf  Preparation of Creams Containing Complex Extracts

Snail mucus, Indian frankincense resin, cream containing aloe vera leaf complex extract was prepared in the composition of Table 1 below.

At this time, Examples 1-1 to 3-5 and Comparative Examples 1 to 6 are the mixture of the snail mucus filtrate, Preparation of the frankincense resin extract, Aloe vera leaf extract of the Preparation Examples 1 to 3 in the composition of Table 1 or Individual extracts (filtrates) were prepared by primary aging at 3 ° C. for 15 hours and secondary aging at 17 ° C. for 8 hours using the same method as Preparation Example 4 above.

Snail Mucus Filtrate
(Part by weight) Indian frankincense resin extract
(Part by weight) Aloe Vera Leaf Extract
(Part by weight) Example 1-1 48.0 1.00 5.00 Example 1-2 49.0 1.00 5.00 Example 1-3 50.0 1.00 5.00 Example 1-4 51.0 1.00 5.00 Example 1-5 52.0 1.00 5.00 Example 2-1 50.0 0.50 5.00 Example 2-2 50.0 0.75 5.00 Example 2-3 50.0 1.00 5.00 Example 2-4 50.0 1.25 5.00 Example 2-5 50.0 1.50 5.00 Example 3-1 50.0 1.00 4.50 Example 3-2 50.0 1.00 4.75 Example 3-3 50.0 1.00 5.00 Example 3-4 50.0 1.00 5.25 Example 3-5 50.0 1.00 5.50 Comparative Example 1 55.0 0.00 0.00 Comparative Example 2 0.00 3.00 0.00 Comparative Example 3 0.00 0.00 7.00 Comparative Example 4 54.5 1.50 0.00 Comparative Example 5 0.00 1.50 5.50 Comparative Example 6 50.5 0.00 5.50

Other components other than the active ingredient of Table 1 are as shown in Table 2 below.

Other Ingredients Skin protection Hydrolyzed Pea Protein, Shea Butter, Castor Oil, Hydrogenated Castor Oil, Jojoba Seed Oil, Argan Cannel Oil, Olive Oil Moisturizing Hollyhock extract, hydroxyethyl urea, trehalose Soothing skin Centella asiatica extract, Root-root root extract, Golden extract, Green tea extract, Spanish licorice root extract, Matricaria flower extract, Rosemary leaf extract, March extract, Grapefruit extract wrinkle improvement Adenosine Other Cappertrippeptide-1, grapefruit peel oil, butylene glycol, beeswax, allantoin, arginine, panthenol, sodium hyaluronate, tocopheryl acetate, hydrolyzed glycosaminoglycan,

< Experimental Example  1> Cell Regeneration Efficacy Assessment 1

Human keratinocytes HaCaT 10000 cells / well, normal human fibroblast 6000 cells to determine the cell regeneration effect of the cream prepared in Examples 1-1 to 3-5 and Comparative Examples 1 to 6 / Well was planted in 96-well plate. Before treating the cream, the medium was exchanged with a medium without FBS and incubated for 1 hour. Thereafter, the medium was removed, and the test substance in which 2 μg / ml of the cream prepared in Examples 1-1 to 3-5 and Comparative Examples 1 to 6 was dissolved in a medium containing 1% of FBS was treated. At this time, the medium containing 10% FBS was used as a positive control. After 48 hours of treatment with the test substance, WST-1 was treated with 100 μl / well. WST-1 was treated and reacted in a 37 ° C. 5% CO ₂ incubator for 3 hours. After incubation for 3 hours, the absorbance at 450 nm was measured using a microplate reader to calculate the cell proliferation rate using the following 1% FBS treated group as 100%.

Cell proliferation rate (%) Human keratinocytes Human fibroblast Untreated group 10 12 Control (1% FBS) 100 100 Positive control group (10% FBS) 165 150 Example 1-1 132 130 Example 1-2 146 141 Example 1-3 152 148 Example 1-4 147 140 Example 1-5 130 129 Example 2-1 131 128 Example 2-2 144 139 Example 2-3 152 148 Example 2-4 146 140 Example 2-5 132 128 Example 3-1 130 129 Example 3-2 145 141 Example 3-3 152 148 Example 3-4 147 142 Example 3-5 132 130 Comparative Example 1 118 116 Comparative Example 2 120 117 Comparative Example 3 119 115 Comparative Example 4 127 124 Comparative Example 5 126 126 Comparative Example 6 128 125

From the results shown in Table 3, the snail mucus filtrate, the Indian frankincense resin extract, the aloe vera leaf extract, and a combination extract thereof according to the present invention showed significant cell regeneration efficacy compared to 1% FBS for two cell groups. In the cream treatment group containing the complex extracts of Examples 1-1 to 1-5 compared to Comparative Examples 1 to 6, a better cell regeneration effect was observed, and in particular, Examples 1-3, 2-3 and 3-3 The highest cell proliferation was observed when the cream prepared in

< Experimental Example  2> Evaluation of skin regeneration and skin improvement efficacy

After dividing the group into 10 persons per evaluation group for women in their 30s to 50s who do not have any symptoms of current skin allergy, the cream prepared in Examples 1-1 to 3-5 and Comparative Examples 1 to 6 of the present invention is once daily Application was made for 8 weeks. The skin condition before and after 8 weeks of use was evaluated, and the average was calculated to evaluate skin elasticity improvement and skin regeneration effect.

The evaluation criteria were 1 to 5 points, and the skin condition for skin hydration, skin elasticity and skin regeneration was 1 point for 'very bad', 2 points for 'slightly worse', 3 points for 'no change', 'Slightly improved' was designated as 4 points, and 'very improved' was marked as 5 points. The experimental results are shown in Table 4 below.

Skin hydration Skin elasticity Skin regeneration Example 1-1 3.7 3.8 3.6 Example 1-2 4.5 4.5 4.4 Example 1-3 4.9 4.8 4.9 Example 1-4 4.5 4.6 4.6 Example 1-5 3.8 3.9 3.7 Example 2-1 3.9 3.8 3.7 Example 2-2 4.6 4.5 4.6 Example 2-3 4.9 4.8 4.9 Example 2-4 4.5 4.6 4.5 Example 2-5 3.8 3.8 3.6 Example 3-1 3.8 3.7 3.6 Example 3-2 4.5 4.6 4.5 Example 3-3 4.9 4.8 4.9 Example 3-4 4.6 4.5 4.6 Example 3-5 3.7 3.8 3.6 Comparative Example 1 3.4 3.3 3.4 Comparative Example 2 3.3 3.4 3.1 Comparative Example 3 3.4 3.3 3.0 Comparative Example 4 3.6 3.5 3.5 Comparative Example 5 3.5 3.6 3.5 Comparative Example 6 3.5 3.5 3.1

As shown in Table 4, the snail mucus filtrate, Indian frankincense resin extract, aloe vera leaf extract each compared to the case of using the cream of Comparative Examples 1 to 6 containing only one or a combination of each, snail mucus filtrate, Indian frankincense When the cream of Examples 1-1 to 3-5 including a composite extract of a resin extract and aloe vera leaf extract all three were used, the skin moisturizing, skin elasticity improvement and skin regeneration effects were better. In particular, when the cream of Examples 1-3, 2-3, and 3-3 was applied, the effect was found to be the best.

< Experimental Example  3> Evaluation of skin damage improvement efficacy

After dividing the group into five subjects per evaluation group, the creams prepared in Examples 1-1 to 3-5 and Comparative Examples 1 to 6 immediately after the laser treatment such as acne treatment and freckle removal were applied to the affected part. Here, the subjects were to apply the creams prepared in Examples 1-1 to 3-5 and Comparative Examples 1 to 6 twice a day to the treatment site for 2 weeks, the size of the lesion site before and after using the product 2 weeks Each was measured to calculate the degree of skin damage. However, the procedure was performed within the range of not more than 50mm horizontally and vertically. The rate of reduction of lesion size measured two weeks after the procedure compared to immediately after the procedure was calculated and shown in Table 5 below.

Lesion size reduction rate (%) Example 1-1 65.2 Example 1-2 79.1 Example 1-3 89.5 Example 1-4 81.6 Example 1-5 67.0 Example 2-1 66.2 Example 2-2 79.6 Example 2-3 89.5 Example 2-4 80.2 Example 2-5 65.6 Example 3-1 65.8 Example 3-2 79.7 Example 3-3 89.5 Example 3-4 80.4 Example 3-5 66.1 Comparative Example 1 51.0 Comparative Example 2 45.2 Comparative Example 3 46.3 Comparative Example 4 56.4 Comparative Example 5 52.2 Comparative Example 6 49.5

Subjects who applied the creams of Examples 1-1 to 3-5 visually confirmed the extent of skin regeneration at the site of treatment at the visit after 2 weeks, while the lesion area was reduced by 65% or more, whereas Comparative Example 1 In the case of subjects to which the creams of 6 to 6 were applied, the skin regeneration effect was relatively weak, and it was confirmed that a scab was formed at the treatment site. In particular, it was confirmed that the skin regeneration effect is best when the cream of Examples 1-3, 2-3 and 3-3 is applied.

< Experimental Example  4> Evaluation of skin regeneration efficacy according to aging conditions

The skin regeneration efficacy of the composite extract prepared by varying the aging conditions of Preparation Example 4 was evaluated.

Specifically, the mixture of the snail mucus filtrate, Indian frankincense resin extract, aloe vera leaf extract prepared by the method of Preparation Examples 1 to 3 to 50.0, 1.0 and 5.0 parts by weight, respectively, the temperature (° C) of Table 6 and The composite extract was prepared by performing primary and secondary aging under the conditions of time (h), and the cream of Examples 4-1 to 4-23 was prepared by mixing the complex extract and the other ingredients of Table 2.

Using the same method as in Experimental Example 2, the creams of Examples 4-1 to 4-23 were applied, and the results of skin moisturizing, skin elasticity, and skin regeneration were shown in Table 6 below.

Primary ripening condition Second maturation condition Skin improvement Temperature time Temperature time Skin hydration Skin elasticity Skin regeneration Example 4-1 One 15 17 8 4.0 3.9 3.7 Example 4-2 2 15 17 8 4.5 4.6 4.5 Example 4-3 3 15 17 8 4.9 4.8 4.9 Example 4-4 4 15 17 8 4.6 4.5 4.6 Example 4-5 5 15 17 8 3.9 4.0 3.7 Example 4-6 3 4 17 8 3.9 3.9 3.6 Example 4-7 3 10 17 8 4.5 4.5 4.6 Example 4-8 3 15 17 8 4.9 4.8 4.9 Example 4-9 3 20 17 8 4.6 4.5 4.5 Example 4-10 3 24 17 8 4.1 3.9 3.8 Example 4-11 3 15 10 8 3.9 3.9 3.7 Example 4-12 3 15 13 8 4.5 4.5 4.6 Example 4-13 3 15 17 8 4.9 4.8 4.9 Example 4-14 3 15 21 8 4.6 4.6 4.5 Example 4-15 3 15 25 8 4.0 3.9 3.7 Example 4-16 3 15 17 4 3.9 4.0 3.6 Example 4-17 3 15 17 6 4.6 4.5 4.5 Example 4-18 3 15 17 8 4.9 4.8 4.9 Example 4-19 3 15 17 10 4.5 4.6 4.6 Example 4-20 3 15 17 12 4.0 4.1 3.8 Example 4-21 3 15 Not performed Not performed 3.7 3.6 3.5 Example 4-22 Not performed Not performed 17 8 3.6 3.6 3.5 Example 4-23 Not performed Not performed Not performed Not performed 3.5 3.5 3.4

As shown in Table 6 above, in the case of using a cream comprising a composite extract prepared by primary aging at 2 to 4 ° C. for 10 to 20 hours and secondary aging at 13 to 21 ° C. for 6 to 10 hours, Creams of Examples 4-21 to 4-23 prepared with a cream comprising a complex extract prepared under the above conditions and a complex extract that was subjected to aging only once or under aging under conditions of primary or secondary aging. Skin hydration, skin elasticity and skin regeneration were higher. Particularly, when the cream including the composite extract prepared by first aging at 3 ° C. for 15 hours and then second aging at 17 ° C. for 8 hours, the skin moisturizing, skin elasticity improvement and skin regeneration effects are the best. Confirmed.

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

Claims (8)

Based on 1 part by weight of Boswellia serrata resin extract, 49-51 parts by weight of snail mucus filtrate, and 4.75-5.25 parts by weight of Aloe barbadensis leaf extract were prepared. Step (step 1);
Preparing a first aged mixture of the mixture of step 1 at 2-4 ° C. for 10-20 hours (step 2); And
Preparing a second aged product of the second aged product of the second step aged at 13-21 ° C. for 6-10 hours (step 3);
Cosmetic composition for skin regeneration, characterized in that it is prepared, including.
The method of claim 1,
The snail mucus filtrate is a cosmetic composition, characterized in that the filtrate collected and filtered by snail mucus secreted from the snail.
The method of claim 1,
The Indian frankincense resin and aloe vera leaf are each extracted using at least one extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon solvent, or a mixed solvent thereof. Cosmetic composition characterized in that.
delete delete The method of claim 1,
The cosmetic composition is a lotion, nourishing lotion, nourishing cream, hydrating cream, massage cream, essence, paste, mask pack, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, makeup base, wax and spray Cosmetic composition, characterized in that at least one formulation selected from the group consisting of.
delete delete