KR102233707B1 - Cosmetic composition comprising natural extract and natural powder - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a natural extract and natural powder. Specifically, the present invention relates to a composition for improving skin, such as promoting beneficial bacteria in the skin, relieving swelling, etc., which contains an Alcea rosea root extract, a Panax ginseng extract, a Lavandula angustifolia flower powder, and peppermint leaf powder.

Description

Cosmetic composition containing natural extract and natural powder TECHNICAL FIELD [COSMETIC COMPOSITION COMPRISING NATURAL EXTRACT AND NATURAL POWDER}

The present invention relates to a cosmetic composition comprising a natural extract and a natural powder, and specifically, to a composition for improving skin such as enhancing beneficial bacteria in the skin, including hollyhock root extract, ginseng extract, lavender flower powder, and peppermint leaf powder, relieving swelling, etc. About.

The skin of the human body functions as a primary barrier that protects the human body from external environmental stimuli that are harmful to the skin, such as ultraviolet rays, various pollutants and pollutants, or changes in temperature and humidity. The skin is divided into three layers: the epidermis, the dermis and the subcutaneous tissue, and the epidermis is again classified into the stratum corneum, granule layer, stratum corneum, and basal layer. It is very important cosmetically.

Epidermal cells undergo a differentiation process called keratinization, and the cells pass through the basal layer, the play layer, the granule layer, and the stratum corneum in order, and repeat the process of dropping out as keratinocytes constituting the outermost layer. The keratin layer functions as an outer barrier, and dead keratin cells are removed from the skin and replaced with new keratin cells. The flat-shaped keratin cells are connected to each other to keep strong and at the same time show flexibility as a whole, and keratin cells are produced sequentially and in succession.

Since the oldest keratinocytes in the outermost layer are continuously removed, the epidermis always maintains a constant thickness. This turnover cycle differs depending on the body part, age, sex, health status, etc., but the replacement cycle of the epidermis in normal skin is about 4 weeks. However, if this keratinization process is not normal, keratinocytes do not fall off, resulting in thickening of the stratum corneum on the surface of the skin or causing skin roughness, and the skin becomes dull, resulting in poor makeup, or an overall lack of vitality.

Accordingly, in recent years, there is an increasing need for the development of a new composition for external application for skin that can remove toxins, moisturize, and give a feeling of elasticity to the skin without accompanying irritation, erythema, redness, and the like when applied to the skin.

Korean Patent Application Publication No. 10-2008-0079489

An object of the present invention is to provide a cosmetic composition for promoting beneficial bacteria or swelling, including hollyhock root extract, ginseng extract, rosemary leaf powder, lavender flower powder, and peppermint leaf powder.

One aspect of the present invention relates to a cosmetic composition for promoting beneficial bacteria or swelling, including hollyhock root extract, ginseng extract, rosemary leaf powder, lavender flower powder, and peppermint leaf powder.

In the present invention, " Alcea rosea " has been used as a medicinal material for symptoms such as baekdaeha, lower abdominal coldness, bowel movements, hematopoiesis, etc., and the effect of collagen biosynthesis in combination with self-cultivation (jichi root), gubaek (dianthus flowers), etc. , It is known as a natural herbal medicine having effects such as inhibition of collagenase expression, inhibition of elastase expression, and inhibition of melanin production.

In the present invention, "Ginseng ( Panax ginseng CA Meyer )" is a perennial herb belonging to the genus Ogapi and Panax, and has been used for centuries in Asia, especially in Korea, China, and Japan as a traditional medicine. Ginseng is an active ingredient, including ginsenosides, polyacetyls, alkyloids, phenolic compounds, polysaccharides, flavonoids, peptides, fatty acids, etc., and is known as a natural medicine with antibacterial properties.

In the present invention, " Rosmarinus officinalis " is a dicotyledonous plant, an evergreen house belonging to the Lamiaceae family, and is a plant native to southern Europe. Rosemary contains various minerals, so it has been used as a material for anti-inflammatory and herbal teas. In particular, rosemary leaves are often used as herbal teas and aromatherapy oils by clearing hair with a unique scent. Recently, it has been known that rosemary extract has antibacterial and antioxidant activity depending on the site, and its efficacy is proven as a raw material for cosmetics and the like.

In the present invention, "Lavender ( Lavandula )" is an evergreen shrub belonging to the Lamiaceae family, originating from the Mediterranean coast, and grows well in a well-drained and sunny place, and is cultivated a lot for ornamental or flavoring. Lavender flowers and leaves have a soothing effect, so they are used to relieve anxiety, headaches, and dizziness, and are used to treat toxins in the human body and as an external application for insect bites.

In the present invention, "peppermint ( Mentha piperita )" is a hybrid of watermint (Mentha aguatica) and spearmint ( Mentha spicata ), and the fragrance is similar to the stinging property of pepper. Menthol, a major component of peppermint essential oil, cools the skin and mucous membranes, is effective in antibacterial and pain relief, so it is used as an edible, medicinal, and fragrance, and is known to have an effect of soothing the skin.

In the present invention, the term "promoting beneficial bacteria" means ^{that there are more than 2 million microorganisms of about 1000 types per 2 cm 2} in the skin, of which it promotes the promotion of beneficial bacteria in the skin and activates the growth of the beneficial bacteria to maintain the balance of microorganisms in the skin. it means.

In the present invention, "swelling" refers to a state in which the body is swollen, and swelling relief means to relieve or subside the swelling of a part of the body or systemic. For example, it may be to relieve swelling compared to usual, such as eyes, cheeks, and under the chin on the face.

In the present invention, it was found that the natural extract including the extract of the hollyhock root and the ginseng extract is excellent in skin improvement effect, and in particular, it is effective in maintaining the microbiome balance and relieving facial swelling by enhancing the activity of beneficial bacteria in the skin.

In addition, the powder of rosemary leaves, lavender flowers, and peppermint leaves, finely crushed from natural plants, provides a peeling effect and at the same time soothes the sensitive skin while providing moisture and nutrition to the skin. It is intended to provide a mixed cosmetic composition.

The composition may further include an extract of Matrica flower, an extract of ginkgo biloba, and an aloe vera extract.

In an embodiment of the present invention, a cosmetic composition comprising natural extracts of hollyhock root and ginseng and natural powders of rosemary leaf, lavender flower and peppermint leaf promotes the growth of beneficial skin bacteria, and it has been confirmed that the effect of enhancing beneficial bacteria activity is excellent. (Table 2).

In one embodiment of the present invention, as a result of preparing a cream using the natural extract and natural powder and applying it to the skin, the reduction in facial volume was significantly increased, and it was confirmed that there is an excellent effect in removing swelling (Table 4).

The above results show that the combination of the two or more natural extracts and natural powders improves the environment in the skin and removes toxins to improve the condition of the skin and face.

The extract may be obtained by extraction alone or with a mixed solvent, and any known solvent may be used.

Specifically, the extract is from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1, 3-butylene glycol It may be obtained by extraction with one or more selected solvents alone or in a mixed solvent, but is not limited thereto.

In addition, the extract may be extracted from natural, hybrid or variant plants of hollyhock, ginseng, Matrica flower, ginkgo and/or aloe vera, and may be extracted from cultures of plant tissues.

The method of extracting the extract of the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, and these may be performed alone or in combination of two or more methods.

In the present invention, the extract extracted with hot water or cold needle is filtered to remove suspended solid particles, for example, nylon, etc. to filter the particles, or after filtration using a freeze filtration method, etc., or use as such, or freeze drying, hot air drying, It can be used after drying by spray drying or the like.

The composition may further include one or more uses selected from the group consisting of improving skin elasticity and relieving redness.

In the present invention, the term "skin elasticity" is represented by elastic fibers composed of elastin present in the dermal layer. These elastic fibers have a very low elastic modulus like rubber, so they are easily deformed even by a small force, and the force must have been removed. When it comes back to its original shape easily. In addition, the elastic fiber has a form in which microfibrils are embedded in an amorphous substrate called elastin, and elastin is a very unique found only in elastic fibers called desmosine and isodesmosne derived from lysine. It is a protein composed of amino acids. These desmosin and isodesmosin form cross-links in long peptide chains, and this structure gives elastin a rubber-like property.

In the present invention, "improving skin elasticity" means that elastic fibers composed of elastin exist together with collagen fibers called collagen, and the elasticity of the skin is maintained or increased in a state in which elastin and collagen are sufficiently present.

In the present invention, "relieving redness" refers to returning the skin that has been reddened by an increase in temperature, sensitivity, or external stimuli to a normal epithelial skin state, or to relieve the degree of burning or reddening of the skin.

In an embodiment of the present invention, as a result of applying the cream containing the natural extract and the natural powder to the face at regular intervals, it was confirmed that the density of the dermal layer in the skin increased and the elasticity was increased (Table 5).

In addition, in an embodiment of the present invention, it was confirmed that the content of peppermint leaf extract can provide a soothing effect to sensitive skin and relieve redness together with the peeling effect of natural powder (Table 6).

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, spray, softening water, emulsion, makeup base, essence, ampoule, soap, liquid detergent, bath agent, sunscreen cream, sun oil , Suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray can be prepared in a formulation selected from the group consisting of, It is not limited thereto.

The cosmetic composition of the present invention may additionally contain one or more cosmetically acceptable carriers blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners , A chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately mixed, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream, or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. It may include a propellant such as locarbon, propane/butane or dimethyl ether, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but the present invention is not limited thereto. These may be used alone or in combination of two or more.

Specifically, in the cosmetic composition as described above, the natural extract and natural powder may be included in an amount of 0.0001% to 30.0% by weight based on the total weight of the cosmetic composition. More specifically, it may be included in 0.005% to 20% by weight. There is an advantage of showing an excellent skin improvement effect within the above range, and there is an advantage of stabilizing the formulation of the composition.

The hollyhock root extract, ginseng extract, rosemary leaf powder, lavender flower powder, and peppermint leaf powder of the present invention not only have an excellent effect on enhancing skin beneficial bacteria, improving swelling, but also improving skin elasticity and relieving skin redness. Therefore, the composition of the present invention including the same can exhibit an excellent skin beauty enhancing effect, and thus can be usefully used as a cosmetic material such as cosmetics.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, but it is obvious that the present invention is not limited by the following examples.

Preparation Example 1. Preparation of natural extracts (plate flower, ginseng, Matrica flower, ginkgo biloba, aloe vera)

After washing hollyhock, ginseng, Matrica flowers, ginkgo biloba, and aloe vera, they were completely dried at room temperature and pulverized to obtain 100 g of each of the pulverized products. Each pulverized product was subjected to immersion extraction for 7 days at room temperature using 70% ethanol in a volume of 20 to 100 g as a solvent. The extract was filtered through a 250 mesh and 3 μm filter, concentrated under reduced pressure at 40° C. and then freeze-dried to obtain a solid content of the extract.

Preparation Example 2. Preparation of natural powder (rosemary leaves, lavender flowers, peppermint leaves)

After washing rosemary leaves, lavender flowers, and peppermint leaves with water, they were completely dried at room temperature and finely pulverized to obtain 100 g of each pulverized product.

Examples and Comparative Examples

In this example, a cosmetic composition containing the natural extract of Preparation Example 1 and the natural powder of Preparation Example 2 was prepared. All of the raw materials of Examples and Comparative Examples described in Table 1 were added, completely dissolved at 80°C, and stirred at 1500 rpm for 5 minutes with a homogenizer.

ingredient
[Content (parts by weight)] Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Hollyhock extract 12 12 - 12 12 Ginseng extract 6 2 18 6 6 Matrica flower extract - 2 - - - Ginkgo biloba extract - One - - - Aloe vera extract - One - - - Rosemary leaf powder 3 3 3 3 3 Lavender flower powder 6 6 6 - 9 Lavender leaf powder - - - 6 - Peppermint leaf powder 3 3 3 3 -

Experimental Example 1. Cytotoxicity confirmation

In order to verify the skin safety of the samples of Examples and Comparative Examples, an MTT assay experiment was performed on fibroblasts (HDF) and keratinocytes (HaCaT).

MTT{3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide} was performed by modifying Mosmann's method of measuring cell viability using a reagent. Fibroblasts and keratinocytes were aliquoted into 96-well plates at ^{a concentration of 1×10 4} cells/well, respectively, and cultured at 37° C. and 5% CO ₂ for 48 hours. The culture medium was removed and the samples were cultured in a medium treated by concentration for 48 hours, and then the medium was removed and washed repeatedly with PBS (Phosphate buffered saline). MTT was dissolved in PBS at 5 mg/mL, 50 μL was added, and incubated for 48 hours at _{37°C and 5% CO 2.} 100 L of DMSO (dimethyl sulfoxide) was added per well, and after stirring for 10 minutes, absorbance was measured at 540 nm.

As a result, cytotoxicity was not confirmed at all concentrations of the samples of Examples 1 and 2 and Comparative Examples 1 to 3, and such results indicate that the cosmetic composition of the present invention is harmful to the human body and is excellent in human stability. It suggests that it can be used.

Experimental Example 2. Confirmation of beneficial bacteria enhancing efficacy

Experiments were conducted to confirm the efficacy of the compositions of Examples and Comparative Examples on proliferation of beneficial skin bacteria. First, Leuconostoc mesenteorides was isolated from Kefir grain using a sorting medium. Using 0.002% BPB-MRS medium, anaerobic culture was performed at 30° C. for 72 hours. Thereafter, lactic acid bacteria colonies were selected and then identified by performing 16S rRNA sequencing.

Two days ago, the turbidity of the bacteria that had been awakened beforehand was adjusted to 2.0, and then injected into a tube containing 3 mL of MRS broth ^{, diluted with 10 1} CFU/mL, and the initial number of bacteria was measured. Thereafter, 5 mg of the compositions of Examples and Comparative Examples were added to 3 mL MRS broth, respectively, and sonicated for 5 minutes (operation of 50 seconds, intervals of 10 seconds, repeated 5 times), and 0.1% of DMSO was injected. After incubation, the number of bacteria (CFU/mL) was measured.

division Number of bacteria (CFU/mL) Example 1 3.48 x 10 ⁷ Example 2 3.53 x 10 ⁷ Comparative Example 1 8.50 x 10 ⁶ Comparative Example 2 3.28 x 10 ⁷ Comparative Example 3 3.23 x 10 ⁷

As a result, it was confirmed that the number of Leukonostock mecenteroides strains cultured in Examples 1 and 2 increased. In particular, when compared to the composition of Comparative Example 1 that does not contain the hollyhock root extract, the growth was significantly increased, which shows that the hollyhock root extract has excellent efficacy in promoting beneficial bacteria in the skin.

Experimental Example 3. Swelling Relief Effect Confirmation

Using the above Examples and Comparative Examples, the effect of improving facial swelling was confirmed. Forty women in their 20s to 30s were divided into two groups of 20 each, and one group used Example 1 and Comparative Example 2, and the other group used Example 2 and Comparative Example 2. The creams of Comparative Examples and Examples were applied by dividing in half. The components of the cream are shown in Table 3 below.

ingredient
(Based on total weight of 100 g, unit: g) Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Hollyhock extract 6 6 - 6 6 Ginseng extract 3 One 9 3 3 Matrica flower extract - One - - - Ginkgo biloba extract - 0.5 - - - Aloe vera extract - 0.5 - - - Rosemary leaf powder 1.5 1.5 1.5 1.5 1.5 Lavender flower powder 3 3 3 - 4.5 Lavender leaf powder - - - 3 - Peppermint leaf powder 1.5 1.5 1.5 1.5 - 1,3-butylene glycol 3 3 3 3 3 glycerin 3 3 3 3 3 Carbomer 0.5 0.5 0.5 0.5 0.5 PEG-60 Hydrogenated Castor Oil 0.02 0.02 0.02 0.02 0.02 Polysorbate 60 One One One One One Beeswax 0.05 0.05 0.05 0.05 0.05 Triethanolamine 0.15 0.15 0.15 0.15 0.15 Purified water to 100 to 100 to 100 to 100 to 100

Using a PRIMOS Body device that can measure the skin volume, the skin volume was measured before and after 2 weeks of use, and the difference in skin volume compared to the initial state was calculated to confirm the effect of improving skin swelling.

division Facial volume reduction (swelling improvement) effect (ml) Example 1 7.2 Example 2 7.7 Comparative Example 2 6.1

As a result, as can be seen from Table 4, the swelling improvement effect of Examples 1 and 2 was increased by 20% or more compared to the comparative example, and in particular, the swelling improvement effect of the lower chin and both cheeks was excellent.

Experimental Example 4. Confirmation of elasticity improvement efficacy

Forty women in their 20s to 40s were divided into 4 groups of 10 each, and the cream of Table 3 was applied every morning and evening for 2 weeks. A skin ultrasound analyzer was used to check the skin dermal density by observation inside the skin, and the skin dermal density was measured before and after 2 weeks of use using this. In addition, a 10-point scale was used for the degree of elasticity increase through a questionnaire survey, and the higher the score, the higher the elasticity of the skin. The elasticity was expressed as an average score of 40 people.

division Dermal density increase rate (%) Elasticity (sensory evaluation) Example 1 39 8.2 Example 2 45 8.8 Comparative Example 1 31 6.0 Comparative Example 2 28 5.9 Comparative Example 3 37 7.9

As a result, as can be seen in Table 5 above, it can be seen that the effect of increasing skin dermal intimacy of Examples 1 and 2 containing all of the hollyhock extract, ginseng extract, rosemary leaf powder, lavender flower powder, and peppermint leaf powder is excellent. Through this, it was confirmed that the cosmetic composition of the present invention using a combination of the natural extract and natural powder has an effect of increasing the density of the dermal layer, and a feeling of increasing the actual elasticity. From these results, it can be seen that the increase in skin elasticity also helps relieve skin swelling.

Experimental Example 5. Confirmation of redness improvement efficacy

In order to analyze the efficacy of the compositions of Examples 1 and 2 and Comparative Examples 1 to 3, the cream of Table 3 was evaluated for the sedation effect by relieving redness in 10 women in their 20s to 40s. The test subjects were prohibited from using any functional cosmetics and basic cosmetics such as eye cream and anti-aging cream, which may affect the test results, and treatments such as packs and massages were also prohibited.

The experiment was conducted in a stable state under constant temperature and humidity (temperature: 22±1℃, humidity: 45±5%) for 30 minutes after washing every morning and evening for 4 weeks. Japan) was applied.

After stabilizing under the above conditions, an appropriate amount of the cream formulations of Examples and Comparative Examples in Table 3 was taken, evenly spread over the face area, and absorbed. Measurements using a spectrophotometer and an anterior-eye imaging system were made before, after 2 weeks of use, and after 4 weeks of use of the test substance. In the evaluation by a spectrophotometer, the left cheek area was measured three times in a row, and the average value was calculated. The measured value of the spectrophotometer consists of three factors, L*, a*, and b*, and the L* value is brightness, a* value is redness, and b* value is yellowness. Show. As a value to measure the sedation effect by relieving the redness of the test substance, a* value representing the redness of the skin was used for analysis, and as the measured value decreases compared to before use of the test substance, there is a sedative effect by relieving redness Means. The evaluation of the improvement rate of the sedative effect by redness relief was measured using Equation 1 below, and then shown in Table 6.

[Equation 1]

Redness improvement rate (%) = (measured value after use-measured value before use)/measured value before use X 100

division Before use 2 weeks after use After 4 weeks of use Example 1 Measures 12.25 11.38 10.25 Improvement rate (%) - 7.10 16.32 Example 2 Measures 12.50 11.55 10.21 Improvement rate (%) - 7.60 18.32 Comparative Example 1 Measures 12.05 11.55 11.13 Improvement rate (%) - 4.14 7.63 Comparative Example 2 Measures 12.45 11.61 11.05 Improvement rate (%) - 6.74 11.24 Comparative Example 3 Measures 13.55 13.17 12.88 Improvement rate (%) - 2.8 4.9

As a result, as can be seen in Table 6 above, in Comparative Example 3 not containing peppermint leaf powder, the redness relief effect was insignificant, whereas in Examples 1 and 2, it was confirmed that the degree of redness relief significantly increased as the use period increased. I did. This shows that the cosmetic composition of the present invention can provide a peeling effect with natural powder, but without irritation to the skin, and can soothe the redness together. The above description of the present invention is for illustration purposes only, and the technology to which the present invention pertains. Those of ordinary skill in the art will understand that it is possible to easily transform into other specific forms without changing the technical spirit or essential features of the present invention. Therefore, it should be understood that the embodiments described above are illustrative and non-limiting in all respects. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention.

Claims (5)

Containing hollyhock root extract, ginseng extract, rosemary leaf powder, lavender flower powder, and peppermint leaf powder,
A cosmetic composition for enhancing or relieving swelling of the skin beneficial bacteria, Leuconostoc mesenteorides. The method of claim 1,
The composition is a cosmetic composition further comprising a Matrica flower extract, ginkgo biloba extract and aloe vera extract. The method of claim 1,
The composition further comprises one or more uses selected from the group consisting of improving skin elasticity and relieving redness, a cosmetic composition. The method of claim 1,
The extract is selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1, 3-butylene glycol. Cosmetic composition extracted with one or more solvents. The method of claim 1,
The composition is a cosmetic composition formulated as one or more from the group consisting of softening lotion, nutrient lotion, cream, lotion, essence, ampoule, gel, oil, foundation, pack, spray, and powder.