KR20120009554A - Cosmetic composition comprising mixed herbal extracts having anti-oxidant activity and anti-allergic activity - Google Patents

Abstract

PURPOSE: A cosmetic composition containing herb extract is provided to ensure anti-oxidation and anti-inflammation, and relieve skin irritation. CONSTITUTION: A cosmetic composition contains herb extract as an active ingredient, including Melissa officinalis, Mentha Suaveolens, Ocimum basilicum, Thymus vulgaris, Mentha spicata, Artemisia dracunculus, Tanacetum vulgare, Origanum vulgare, Lavandula anguistifolia, and Rosemarinus officinalis. The herb extract is prepared using water, glycol of 1-6 carbon atoms, alocohol of 1-4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate, 1,3-butylene glycol, or mixture thereof.

Description

Cosmetic composition containing herbal extracts having antioxidant and anti-allergic effects {COSMETIC COMPOSITION COMPRISING MIXED HERBAL EXTRACTS HAVING ANTI-OXIDANT ACTIVITY AND ANTI-ALLERGIC ACTIVITY}

The present invention relates to a cosmetic composition containing herbal extracts of lemon balm, applemint, basil, thyme, spearmint, tarragon, tanji, oregano, lavender and rosemary with antioxidant and antiallergic activity.

The activity of cells constituting the skin and the ability of the skin to protect against external infectious agents decrease as the ozone layer breaks down, increasing ultraviolet rays reaching the surface, weakening immunity due to stress, and increasing exposure to pollutants such as harmful chemicals.

Accordingly, the skin is sensitive to external stimuli, causing skin troubles and inflammatory reactions such as erythema, edema, and itching. In addition, the substances produced during the inflammatory reaction concomitantly cause pigmentation of the skin and accelerate the breakdown of skin elastic fibers and collagen fibers, thus affecting the increase of skin wrinkles.

Skin irritation that may occur with the use of cosmetics includes irritation such as itching, stinging and burning and inflammatory irritation such as erythema and edema. Such skin irritation may be said that cosmetics penetrate the skin and interact with the epidermis or dermal cells to activate the immune system and cause irritation and inflammatory reactions. In addition, contact dermatitis may be induced as a local inflammatory reaction occurring on the skin by an external irritant, and such contact dermatitis includes irritant contact dermatitis, allergic contact dermatitis, sensory irritation, and phototoxic dermatitis.

Significant amounts of reactive oxygen species (ROS) are produced during the oxidation process for energy production in vivo. Most of these reactive oxygen species are eliminated before being removed in vivo, but instantaneous generation of active oxygen species is generated in large quantities or chronically active oxygen species are generated, and the balance with the antioxidant defense system causes various diseases. Reactive oxygen species damage the skin cells and tissues, as well as destroy the balance of antioxidants and antioxidants consisting of antioxidant and non-enzymatic antioxidants, promote lipid peroxidation, protein oxidation, chain cleavage of collagen and elastin, and promote melanin production, It causes damage to biological components such as oxidation of DNA.

Inflammation is a physiological defense response of biological tissues to external stimuli. Clinically, symptoms such as redness, fever, swelling, pain, and dysfunction are caused by harmful surroundings, such as invasion and mechanical damage of external foreign substances such as bacteria. It is a physiological mechanism to protect the skin from.

Various inflammatory mediators reduce the antioxidant capacity of tissues by activating the production of oxidants, causing damage to tissue's basic metabolic structural factors, causing toxicity, and oxidation of these various cells and tissues produces unique oxides Secondary tissue reactions of reactive oxygen species begin.

Free radicals and reactive nitrogen species (RNS) are associated with inflammatory responses that regulate the actions of various cells in vivo. The main causes of inflammatory reactions are microbial infections, hypersensitivity reactions, physical factors, chemical factors and tissue necrosis. In particular, nitric oxide (NO) produced by nitric oxide synthase (NOS) and histamine and β-hexosaminidase secreted from mast cells are inflammatory reactions. Is a major transporter. Therefore, a substance capable of inhibiting the release of histamine and beta-hexosaminidase secreted from mast cells or reducing the production of nitric oxide can provide a suitable effect for alleviating dermatitis such as atopic dermatitis as well as general inflammatory response. have.

Accordingly, there is a need for the development of functional substances that can improve skin trouble caused by reactive oxygen species and inflammation due to excellent antioxidant and anti-inflammatory effects.

The present inventors have diligently researched natural ingredients with antioxidant and anti-allergic activity. As a result, herbal extracts of lemon balm, applemint, basil, thyme, spearmint, tarragon, tanji, oregano, lavender and rosemary have antioxidant and anti-inflammatory activity. At the same time, it was confirmed that it is possible to improve skin troubles without accompanying skin side effects, and based on this, the present invention was completed.

It is an object of the present invention to provide a cosmetic composition comprising a herb extract having antioxidant, anti-inflammatory and anti-allergic effect as a functional raw material.

In order to achieve the above object, the present invention provides a cosmetic composition containing herbal extracts of lemon balm, apple mint, basil, thyme, spearmint, tarragon, tanji, oregano, lavender and rosemary.

Since the herbal extract of the present invention is excellent in antioxidant, anti-inflammatory and anti-allergic effect, cosmetic composition containing it as a raw material can exhibit an antioxidant, anti-inflammatory, skin irritation relief, skin trouble improvement effect.

1 is a graph showing the cell growth rate (%) of the herbal extract and dry herb extract according to the present invention.
2 is a graph showing the DCF fluorescence intensity according to the treatment according to the herbal extract concentration.
Figure 3 is a graph showing the DCF fluorescence intensity with respect to the time according to the treatment by the concentration of dry herb extract.
Figure 4 is a graph showing the DCF fluorescence intensity with respect to the time according to the treatment by the concentration of fresh herbs extract.
Figure 5 is a graph showing the release amount of beta- hexosaminidase according to the treatment according to the herbal extract concentration.

Herbal extracts of the present invention include extracts of lemon balm, applemint, basil, thyme, spearmint, tarragon, tanji, oregano, lavender and rosemary.

At this time, the extract is obtained by extracting from various organs or parts, it is possible to extract all, such as leaves, flowers, roots, stems, branches, shells and seeds. Preferably leaves are used, most preferably fresh leaves that are not dry.

According to Experimental Examples 3 and 4 of the present invention, the fresh herb extracts obtained from the fresh leaves were more effective in scavenging the active oxygen species (ROS) scavenging effect and the beta-hexosaminidase secretion effect in comparison with the dry herb extract. Excellent.

Raw materials of the herbal extract contained in the composition of the present invention are as follows.

Lemon balm (Melissa officinalis L., Lemon Balm) is also called 'Melissa' and is famous as a wheat plant because fresh and sweet lemon scent attracts bees. Just rub the leaves, the smell of a strong lemon, medicinal, tea, used as a fragrance, especially the brain's activities to enhance memory, relieve depression, fever, sweating effect is effective in recovering cold and fatigue.

Apple Mint ( Mentha Suaveolens , apple mint) is a herb native to Europe. The scent of apples and peppermint is mild, and the leaves are egg-shaped, round, with white hairs and soft. It is also medicinal, which is good for disinfection, healthy tonic, soothing and analgesic.

Basil ( Ocimum basilicum , basil) is a variety of applications in Western cuisine, so called 'herb' and 'herb' herb kitchen herbs. Essential oils obtained by steam distillation of dried leaf and flower parts are mainly used as fragrances for cosmetics, perfumes, soaps, toothpaste, etc., and have ingredients of ocimene and alpha-pinene. It is also used as a medicine for fever, detoxification, diarrhea, constipation, and menstrual irregularities.

Thymus vulgaris L. , thyme) is called musk vine and is used as a spice, with a tangy scent and a slightly bitter taste. Various effects such as antiseptic and antibacterial activity are known, and since ancient times, it has been used as an aphrodisiac because it has been used to store the body or to use it as an antibiotic. Bronchial jingle, expectorant, antibacterial effect.

Spearmint ( Mentha Viridis L. spearmint) is a perennial herb that is native to Europe and grows up to 30-60 cm tall. The flower is named Spear because it resembles a window, and unlike other mints, it has a sweet and fresh scent. Oil has a soothing and analgesic effect that relaxes nerves and muscles, and is known to have a great effect on indigestion, seasickness and nausea.

Tarragon ( Artemisia dracunculus , tarragon) are supposed to be native to Siberia, the leaves are light green, have a mild aroma and taste like anise. Fresh leaves are used as salads, and fresh tarragon vinegar has a unique taste. Essential oil (精油) contains 0.3 ~ 1.0%, the main component is methyl chavicol (metyl chavicol).

Tanacetum vulgare , tansy) grows up to 80-150 cm in height and has a scent like camphor. The leaves are dark green and deeply divided like grass ferns. Because of its unique fragrance, it can be used to make dried leaves into potpourri or to get rid of pet fleas, and it is effective for migraines, skin diseases, bruises and rheumatism.

Origanum vulgare L. ( oregano) is a perennial herb with mint and is called "wild majoram" because of its marjoram-like scent. Medicinal parts are oils extracted from fresh or dried leaves. Characterized by a scent-like scent, the essential oil's cavacrol ingredient has an antibacterial effect and is known to inhibit Helicobacter pylori.

Lavandula anguistifolia , lavender) is used for ornamentals as well as potpourri and various cosmetics because of its aroma of flowers, leaves, stems, etc. Its main constituents include 0.5–1.5% volatile oil, 5–10% tannin, coumarin, flavonoids, and triterpenoids.

Rosemarinus officinalis L. , rosemary) is a herb with needle-like leaves. It is a food ingredient loved by evergreen leaves and distinctive aromas, and is native to the Mediterranean coast. Medicinal parts are oils extracted from fresh leaves of dried eggplants or dried twig ends, which have a strong irritating scent. The main effect is antibacterial, antiviral action is known to be due to the effect of diterpene (diterpene) among the ingredients. Rosemary leaves contain about 2.5% essential oils, the main constituents of which are 27-30% cineole, 16-20% borneole, 10% camphor, and bornyl acetate 2 7% and so on.

Herbal extract of the present invention, the raw material according to the conventional methods known in the art, that is, using a conventional solvent, hot water extraction, room temperature extraction, warming extraction, ultrasonic extraction, supercritical extraction commonly used It can be produced by such a method. At this time, it is preferable to use after mixing each raw material herb using an extraction solvent.

Specifically, using a solvent selected from the group consisting of water, glycols having 1 to 6 carbon atoms, anhydrous or hydrous alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, 1,3-butylene glycol and mixtures thereof Extract. Preferably it is extracted using 35 to 45% (v / v) 1,3-butylene glycol as the extraction solvent. At this time, the usage amount of the extraction solvent is 10 to 30 times the volume amount of the raw material weight, more preferably 20 times.

Extraction temperature and time may vary depending on the amount of sample to be extracted and is heated to extract for 3 to 100 hours at 40 to 100 ℃, or preferably carried out for 1 to 5 days at 50 to 70 ℃. The extraction may be filtered or purified.

In addition, the herbal extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

At this time, the composition ratio of the herbal extract included in the composition of the present invention is not particularly limited, but lemon balm extract 1 to 30% by weight, applemint extract 1 to 30% by weight, basil extract 1 to 30% by weight, thyme extract 1 to 30 Wt%, spearmint extract 1-30%, tarragon extract 1-30%, tangy extract 1-30%, oregano extract 1-30%, lavender extract 1-30% and rosemary extract 1-30% It is preferable to use as. This composition ratio is in the range to secure the effect to be obtained in the present invention, such as antioxidant, anti-inflammatory and anti-allergic, through Experimental Example 1 it was confirmed that the herbal extract having the above-described composition exhibits an excellent antioxidant and anti-inflammatory effect .

Cosmetic composition of the present invention comprises the herbal extract as an active ingredient.

In the composition of the present invention, the content of the extract is 0.001 to 30% by weight, preferably 0.01 to 10% by weight relative to the total weight of the cosmetic composition. If the content of the extract is less than the above range, there is a problem in showing antioxidant, anti-inflammatory and skin irritation-reducing effects, on the contrary, in the case of exceeding the above range, there is no apparent increase in effect due to the increase in content, discoloration and Odor occurs, there is a problem that the stability of the formulation is lowered.

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to herbal extracts as active ingredients, such as conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, and Carrier.

The compositions of the present invention may be prepared in any formulations commonly prepared in the art, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of softening lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

[ Example ]

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Manufacturing example  One

Herbs used for extraction were used undried fresh leaves.

Lemon balm, applemint, basil, thyme, spearmint, tarragon, tanji, oregano, lavender and rosemary were each washed with purified water and then drained. 50 g of each raw material was prepared and placed in 1000% of 40% 1,3-butylene glycol, and extracted by heating at 60 ° C. for 96 hours using ultrasonic waves, filtered with 300 mesh filter cloth, and left to mature at 10 ° C. for 8 days. It was then filtered through Whatman 5 filter paper. The filtrate was dried with a rotary evaporator at 70 ° C. to obtain respective extracts.

Experimental Example  1: Antioxidant and Antiallergic Effect According to Herb Composition

In order to confirm the antioxidant and anti-allergic effects according to the herbal composition and content, the herbal extract obtained in Preparation Example 1 was mixed in the composition ratio according to Table 1 below to determine the efficacy. Antioxidant effect was tested in Experimental Example 2, anti-allergic effect using the same method as described in Experimental Example 3, the results are shown in Table 2 below.

extract

(weight%) Lemon balm Apple Mint basil time Spearmint Tarragon Tanji oregano lavender Rosemary Reference Example 1 20 0 0 20 20 0 0 20 10 10 Reference Example 2 20 10 0 10 10 0 0 20 10 10 Reference Example 3 20 10 0 10 10 0 10 20 10 10 Reference Example 4 20 10 10 10 10 10 0 10 10 10 Reference Example 5 10 10 10 10 10 10 10 10 10 10 Reference Example 6 5 35 5 5 10 5 5 10 10 10 Reference Example 7 5 5 35 10 5 5 5 10 10 10 Reference Example 8 10 5 5 35 10 5 5 5 10 10 Reference Example 9 10 5 5 10 35 5 10 10 5 5 Reference Example 10 5 10 10 5 5 35 10 5 5 10 Reference Example 11 5 15 10 5 5 10 35 5 5 5 Reference Example 12 5 10 10 5 5 10 10 35 5 5 Reference Example 13 5 10 10 5 5 10 10 5 35 5 Reference Example 14 5 10 10 5 5 10 10 5 5 35

division Application concentration (μg / ml) DCF fluorescent intensity
(Antioxidant) Beta-hexomidase secretion inhibition (%)
(Antiallergic) Reference Example 1 100 495.46 91.24 Reference Example 2 100 462.39 86.43 Reference Example 3 100 437.22 87.20 Reference Example 4 100 396.08 84.31 Reference Example 5 100 345.51 67.62 Reference Example 6 100 384.77 76.38 Reference Example 7 100 379.32 74.40 Reference Example 8 100 363.48 72.82 Reference Example 9 100 374.33 74.11 Reference Example 10 100 376.71 73.77 Reference Example 11 100 377.92 73.95 Reference Example 12 100 365.06 73.21 Reference Example 13 100 359.41 70.51 Reference Example 14 100 362.88 72.67

Experimental Example  2: confirm the safety of herbal extracts

In order to verify the primary safety of the herbal extract according to the present invention as a cosmetic, normal human dermal fibroblasts (Human dermal fibroblast cells) were cultured and subjected to cell viability by the MTT assay method. DMEM (Dulbecco's Modified Eagle's Medium) with 10% bovine serum was used as a medium to inoculate cells in 96-well plates at a concentration of 3000-5000 cells / well, followed by 5% CO ₂ , 37 ℃ was incubated. Extract samples were treated by diluting them in media containing no serum for each concentration.

After removing the supernatant and adding MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) solution, a dye for 0.5% (w / v) cell staining, for 4 hours Were further incubated. The chromosome crystals were dissolved in dimethyl sulfoxide and measured for absorbance at 570 nm for relative comparison. As a control, a culture medium without the extract sample was used. When the cell viability is calculated as a numerical value using Equation 1 below, the relative cell viability effect is shown in FIG. 1 when the cell survival rate (%) of the control group not treated with the extract sample is zero.

[Equation 1]

Cell survival rate (%) = [(absorbance / control absorbance of each extract sample) X100]

1 is a graph showing the cell survival rate (%) of the live herb extract and dry herb extract according to the present invention.

As shown in Figure 1, the fresh herb extract according to the present invention was found to have a high survival rate compared to the dry herb extract was found to be low in cytotoxicity, accordingly, the fresh herb extract is superior to the dry herb extract is found to be superior in safety Could.

Experimental Example  3: intracellular To free radicals (ROS)  Inhibitory effect

In order to investigate the scavenging effect of intracellular Reactive Oxygen Species (ROS) of herbal extracts according to the present invention using oxidation-sensitive dyes 2 ', 7'-dichlorofluorescein diacetate (DCFH-DA) The experiment was performed as follows.

RAW 264.7 cells incubated in fluorescent microtiter 96-well plates were labeled with DCFH-DA 20 μM in Hanks balanced salt solution (HBSS) for 30 minutes in cancer. The cells were then treated with different concentrations of herbal extracts and incubated for an additional hour. After washing the cells three times with PBS, 500 μM H ₂ O ₂ was added. The intensity of the fluorescence signal emitted from 2 ', 7'-dichlorofluorescein (DCF) due to oxidation of DCFH by cellular ROS was time-dependently determined using a GENios fluorescent microtiter reader (Tecan Austria GmbH, Austria). λ excitation = 485 nm, λ emission = 528 nm). After the fluorescence intensity increase was maximized, each well was normalized to cell number using MTT cell viability assay. Treatment effects were plotted and compared to the fluorescence intensities of the control and blank groups. The results are shown in FIG.

2 is a graph showing the DCF fluorescence intensity according to the treatment according to the herbal extract concentration.

Figure 3 is a graph showing the DCF fluorescence intensity with respect to the time according to the treatment by the concentration of dry herb extract.

Figure 4 is a graph showing the DCF fluorescence intensity with respect to the time according to the treatment by the concentration of fresh herbs extract.

Referring to Figures 2-4, monitoring the DCF fluorescence intensity for 100 minutes, showed that the radical-mediated oxidation increased with incubation time. However, when herbal extracts were treated, the fluorescence intensity of DCF decreased time-dependently, and all concentrations of herbal extracts (10, 50, and 100 μg / mL) were free radicals compared to the control (H ₂ O ₂ only control). Scavenging activity was shown. In particular, DCF fluorescence intensity decreased more than about 15% in the fresh herb extract than the dry herb extract. This means that the herbal extract according to the present invention has a strong intracellular direct radical scavenging effect in a dose- and time-dependent manner, thereby showing an excellent antioxidant effect.

Experimental Example  4: beta- Hexosaminidase  Secretion inhibitory effect

In order to evaluate the dermatitis and allergic effect of the herbal extract according to the present invention, the change in secretion amount of beta-hexosaminidase was measured.

Beta-hexosaminidase (β-hexosaminidase) is an enzyme contained in mast cells, which are released together with histamine when mast cells are activated by an immune response. Thus, beta-hexosaminidase can be used as an indicator of degranulation of mast cells.

Rat basophil leukocytes, RBL-2H3 cells, were prepared in EAG (Eagles Minimum Essential Medium) medium with 10% fetal bovine serum (FBS), 1% glutamine, 100 units / mL penicillin and 100 μg / mL streptomycin. ) Was added and incubated in an incubator (37 ° C., 5% CO ₂ ).

Cells were harvested by trypsin / versene when grown to about 80% of the bottom of a 100 mm dish, and incubated for 18 hours with 2 X 10 ⁶ cells per well inoculated into 24 well plates. The medium was removed and the cells were sensitized by reacting the cells with 1 μg / ml of anti-DNP-IgE for 18 hours, and then the samples were pretreated for 1 hour. After 10 minutes of treatment with 10 μg / ml of DNP-BSA, the upper layer was obtained.

50 μl of the obtained supernatant and 50 μl of 5 mM p-nitrophenyl-N-acetyl-β-glucosamide dissolved in 0.2 M citrate buffer (pH 4.5) were reacted in an incubator for 30 minutes. 1N HCl was added to stop the reaction, and the release amount of beta-hexosaminidase was measured at 405 nm. The same experiment was performed with the supernatant obtained by hemolysis of cells with 0.1% Triton X-100 to determine the release of total beta-hexosaminidase. The results are shown in FIG.

Figure 5 is a graph showing the release amount of beta- hexosaminidase according to the treatment according to the herbal extract concentration.

As shown in Figure 5, the herbal extract treatment group according to the present invention was confirmed to have an anti-allergic effect by concentration-dependently inhibiting the beta-hexosaminidase secretion of RBL-2H3 cells. In particular, the amount of beta-hexosaminidase secretion was reduced by more than about 20% in the fresh herb extract than the dry herb extract. These results suggest that the use of the extract of the present invention can effectively alleviate skin inflammation and allergy.

Hereinafter, examples of the formulation of the present invention include a softening lotion, a convergent lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence and a pack, but the formulation of the cosmetic composition of the present invention is not limited thereto.

Formulation example  1: flexible cosmetics ( skin )

As described in Table 3 below, the flexible longevity containing the herbal extract of Reference Example 5 was prepared according to a conventional method.

ingredient Content (% by weight) Herbal extract One glycerin 5 1,3-butylene glycol 3 Betaine One Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Sodium hyaluronate powder 0.05 ethanol 5 Octyldodec-16 0.2 Polyoxyethylene Cured Castor Oil 0.2 incense Quantity antiseptic Quantity Pigment Quantity Purified water Balance Sum 100

Formulation example  2: nutrition lotion (lotion)

Nutrients containing the herbal extracts of Reference Example 5 were prepared according to a conventional method as shown in Table 4 below.

ingredient Content (% by weight) Herbal extract 3 Glyceryl Stearate SE 1.5 Cetearyl alcohol 1.0 Shea Butter 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil One Mineral oil 5 Squalane 3 Cyclomethicone 2 Dimethicone 0.8 Tocopherol Acetate 0.5 Carbomer 0.12 glycerin 5 1,3-butylene glycol 3 Sodium hyaluronate powder 0.05 Triethanolamine 0.12 incense Quantity antiseptic Quantity Pigment Quantity Distilled water Balance Sum 100

Formulation example  3: nutrition cream

The nutrition cream containing the herbal extract of Reference Example 5 was prepared according to a conventional method as shown in Table 5 below.

ingredient Content (% by weight) Herbal extract 3 Lipophilic monostearate 2 Cetearyl alcohol 2 Stearic acid 1.5 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hydrogenated Polyisobutene One Squalane 3 Mineral oil 5 Cyclomethicone 5 Dimethicone One Tocopherol Acetate 0.5 glycerin 5 Betaine 3 Triethanolamine One Xanthan Gum 0.05 incense Quantity antiseptic Quantity Pigment Quantity Distilled water Balance Sum 100

Formulation example  4 : Massage  cream

A massage cream containing the herbal extract of Reference Example 5 was prepared according to a conventional method as shown in Table 6 below.

ingredient Content (% by weight) Herbal extract 3 Lipophilic Monostearic Acid Glycerin 1.5 Cetearyl alcohol 1.5 Stearic acid One Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl Isosterate 5 Squalane 5 Mineral oil 35 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6 1,3-butylene glycol 3 Triethanolamine 0.3 incense Quantity antiseptic Quantity Pigment Quantity Distilled water Balance Sum 100

Formulation example  5: essence

An essence containing the herbal extract of Reference Example 5 was prepared according to a conventional method as shown in Table 7 below.

ingredient Content (% by weight) Herbal extract 3 glycerin 6 Betaine 5 PEG 1500 2 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Hydrogenated Lecithin 0.6 Hydroxyethylcellulose 0.1 Sodium hyaluronate powder 0.08 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.2 Ceramide 0.2 Octyldodecanol 3 Squalane 3 Polysorbate 60 0.4 Glyceryl Stearate SE 1.5 incense Quantity antiseptic Quantity Pigment Quantity Distilled water Balance Sum 100

Formulation example  6: pack

As shown in Table 8 below, a pack containing the herbal extract of Reference Example 5 was prepared according to a conventional method.

ingredient Content (% by weight) Herbal extract One Polyvinyl alcohol 15 Cellulose gum 0.15 glycerin 3 PEG 1500 2 Betaine 2 DL-Panthenol 0.4 Allantoin 0.1 Triethanolamine 0.2 Nicotinamide 0.5 ethanol 6 PEG 40 Cured Castor Oil 0.3 incense Quantity antiseptic Quantity Pigment Quantity Distilled water Balance Sum 100

Claims (6)

Lemon balm (( Melissa officinalis , Applemint ( Mentha Suaveolens ), basil ( Ocimum basilicum ), thyme ( Thymus) vulgaris ), spearmint ( Mentha spicata ), tarragon ( Artemisia dracunculus , Tanacetum vulgare ), Origanum vulgare , Lavandula Anguistifolia ) and a cosmetic composition containing a herb extract of Rosemarinus officinalis as an active ingredient. According to claim 1, wherein the herbal extract is 1 to 30% by weight lemon balm extract, 1 to 30% by weight apple mint extract, 1 to 30% by weight basil extract, 1 to 30% by weight thyme extract, 1 to 30% by weight spearmint extract %, 1 to 30% by weight of tarragon extract, 1 to 30% by weight of tangy extract, 1 to 30% by weight of oregano extract, 1 to 30% by weight of lavender extract and 1 to 30% by weight of rosemary extract. The method of claim 1, wherein the herbal extract is a group consisting of water, glycols having 1 to 6 carbon atoms, alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate, 1,3-butylene glycol and mixtures thereof Cosmetic composition that is extracted with one extractant selected from. The cosmetic composition of claim 1, wherein the herbal extract is extracted by hot water extraction, room temperature extraction, warm extraction, ultrasonic extraction, or supercritical extraction. The cosmetic composition of claim 1, wherein the content of the herbal extract is 0.1 to 20% by weight based on the total weight of the cosmetic composition. The composition of claim 1 wherein the composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, pack, essence, emulsion foundation, wax foundation and Cosmetic composition having one formulation selected from the group consisting of a spray.

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