KR20210072966A - Nanocapsule composition for skin moisturizing or skin inflammatory improvement comprsing encapsulated centella and calamine - Google Patents

Abstract

The present invention relates to a nanocapsule composition for moisturizing the skin or mitigating skin inflammation containing a nanocapsulated centella extract, calamine, a hyaluronic acid complex, a licorice extract, a green tea extract, a cypress extract, and glycerin, and a cosmetic composition and health functional food composition containing the same as an active ingredient. According to the present invention, the nanocapsule composition for moisturizing the skin or mitigating skin inflammation increases skin moisture retention ability to improve a skin barrier function, thereby providing a moisturizing effect on the skin. In addition, the nanocapsule composition has an excellent antibacterial effect against acne-causing bacteria such as Propionibacterium acnes (P.acnes), Staphylococcus aureus (S.aureus) and the like so that the nanocapsule composition can be usefully used as a cosmetic composition for teenagers, who are easily exposed to diseases such as skin troubles and acne.

Description

Nanocapsule composition for skin moisturizing or skin inflammation improvement comprising nanoencapsulated Centella extract and calamine

The present invention relates to a nanocapsule composition for skin moisturizing or skin inflammation improvement comprising nanoencapsulated Centella extract and calamine.

As an organ in contact with the external environment, the skin protects the human body from external physical damage and chemicals, prevents bacteria, fungi, and viruses from penetrating into the human body, and acts as a barrier to water loss. On the other hand, looking at the structure of the skin, it is composed of three layers: the epidermis, dermis, and subcutaneous fat. In particular, the skin becomes elastic and soft by the moisture present in the stratum corneum of the epidermis, and it is known that moisture content of 10% or more is essential to maintain the elasticity of the stratum corneum.

Skin hydration is determined by the equilibrium of water loss in the epidermis by evaporation and water transfer from the epidermis to the dermis by osmotic pressure. Clinically, dry skin has a dry skin surface, lacks elasticity, and is prone to scaling and roughness. In addition, itchy symptoms may appear, and in some cases, symptoms such as dryness and cracking may be accompanied. The level of moisture in the epidermis is highly dependent on intercellular lipids, corneodesmosom, and natural moisturizing factor (NMF).

On the other hand, the inflammatory response in the skin starts as an action to defend against skin damage caused by physical stimuli, chemicals, bacteria, etc., and various immune cells and inflammation-inducing cytokines are involved. TNF-α (tumor mecrosis factor-α), IL-1β (interleukin-1β), IL-6 (interleukin-6), IL-8 (interleukin-8), etc. are representative inflammation-inducing cytokines. In addition, activated macrophages excessively produce nitric oxide (NO) or prostaglandin E2 (PGE2) as well as inflammation-inducing cytokines to further activate the inflammatory process.

Acne (acne vulgaris), which is one of the diseases that inflammatory skin disorders can cause, is an inflammatory skin disease in the sebaceous glands mainly due to hormonal changes occurring during puberty. The causes of acne are excessive sebum production and enlargement of sebaceous glands activated by androgens, proliferation of epidermal keratinocytes in the pilosebaceous glands, incomplete exfoliation of hair follicle keratinocytes and blockage of the sebaceous glands, and Propionibacterium acnes ( Inflammation caused by bacteria called Propionibacterium acnes , P. acnes is well known.

The present invention provides a nanocapsule composition for skin moisturizing or skin inflammation improvement comprising nanoencapsulated Centella extract, calamine, hyaluronic acid complex and licorice extract, green tea extract, cypress extract and glycerin, containing the composition as an active ingredient To provide a cosmetic composition, a health functional food composition.

In addition, the technical problems to be solved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned are clearly to those of ordinary skill in the art to which the present invention belongs from the description below. can be understood

In the present specification, there is provided a nanocapsule composition for skin moisturizing or improving skin inflammation, comprising centella extract, calamine, hyaluronic acid, licorice extract, green tea extract, cypress extract and glycerin.

In the present specification, the centella extract, calamine and hyaluronic acid are at least one selected from the group consisting of chitooligosaccharide, gelatin, pectin, polyethylene glycol, methyl cellulose, sodium carboxymethyl cellulose, hydroxymethylpropyl cellulose and ethyl cellulose. It provides a nanocapsule composition for improving skin moisture or skin inflammation, which is nanoencapsulated with a polymer.

In the present specification, the Centella extract provides a nanocapsule composition for skin moisturizing or skin inflammation improvement, which is a fermented product of Centella hot water extract.

In the present specification, the fermented product is Centella extract Lactobacillus buchneri , Lactobacillus paracasei subsp. tolerans , Lactobacillus harbinensis , Saccharomycopsis fibuligera and Pichia kudriavzevii It provides a nanocapsule composition for improving skin moisture or skin inflammation, which is naturally fermented with one or more strains selected from the group consisting of.

In the present specification, the composition contains 30 to 60 parts by weight of calamine, 20 to 40 parts by weight of hyaluronic acid, 10 to 15 parts by weight of licorice extract, 5 to 10 parts by weight of green tea extract, 3 to 8 parts by weight of cypress extract per 100 parts by weight of centella extract. It provides a nanocapsule composition for skin moisturizing or skin inflammation improvement, including parts by weight, and 5 to 15 parts by weight of glycerin.

In this specification, the composition propionic sludge tumefaciens arc Ness (Propionibacterium acnes, P.acnes) and Staphylococcus aureus nanocapsules composition having an antimicrobial effect on the (Staphylococcus aureus, S.aureus), skin moisture or skin irritation improvement provides

In the present specification, there is provided a cosmetic composition for improving skin moisture or skin inflammation containing the composition as an active ingredient.

In the present specification, the cosmetic composition is a preservative, a stabilizer, a surfactant, a thickener, a solubilizer, a humectant, an emollient, an ultraviolet absorber, a preservative, a disinfectant, an emulsifier, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cooling agent And it provides a cosmetic composition for improving skin moisture or skin inflammation further comprising any one or more selected from the limiting agent.

In the present specification, there is provided a quasi-drug composition for skin moisturizing or skin inflammation improvement containing the composition as an active ingredient.

The nanocapsule composition for skin moisturizing or skin inflammation improvement according to the present invention has a skin moisturizing effect by increasing the skin moisture retention ability to improve the skin barrier function.

In addition, due to its excellent antibacterial effect against acne-causing bacteria such as Propionibacterium acnes , P.acnes and Staphylococcus aureus, S.aureus , adolescents who are easily exposed to diseases such as skin troubles and acne It can be usefully used as a cosmetic composition for people.

1 is a schematic view sequentially showing the steps of preparing a nanocapsule composition for skin moisturizing or skin inflammation improvement according to an embodiment of the present invention.

Hereinafter, a nanocapsule composition for skin moisturizing or skin inflammation improvement comprising nanoencapsulated Centella extract, calamine and hyaluronic acid according to a specific embodiment of the present invention will be described in more detail.

On the other hand, the term "skin barrier improvement" used throughout the specification and claims of the present invention is a unique function of the skin that protects the human body from external physical damage and chemical substances, and prevents bacteria, fungi, viruses, etc. from invading the skin. It should be understood as meaning including all functions of preventing water loss and preventing moisture loss.

The nanocapsule composition for skin moisturizing or skin inflammation improvement according to the present invention includes centella extract, calamine, hyaluronic acid, licorice extract, green tea extract, cypress extract and glycerin.

More specifically, the Centella extract, calamine and hyaluronic acid according to an embodiment of the present invention are chitooligosaccharide, gelatin, pectin, polyethylene glycol, methyl cellulose, sodium carboxymethyl cellulose, hydroxymethylpropyl cellulose and ethyl cellulose. It may be nanoencapsulated with one or more polymers selected from the group consisting of.

Centella asiatica , also called Centella asiatica, is a perennial herb commonly found in the mountains and fields of the southern islands of the Korean Peninsula. The main stem extends sideways and there are two degenerated scale-shaped leaves near the root node. On the other hand, Centella extract is known to improve symptoms related to venous circulation disorders, such as dullness or pain in the lower extremities, and restlessness of the lower extremities, and it is known that external preparations are used for the secondary partial treatment of wounds and skin ulcers. On the other hand, Centella extract is known to have triterpenoid-based saponins and flavonoids including asiaticoside as active ingredients.

Calamine is a pink powder obtained by mixing zinc oxide (zinc oxide) and iron oxide. It is mainly used as a base for shaking lotions in cosmetics. It is known to be used for skin care and skin care after sunburn.

Hyaluronic acid is one of the complex polysaccharides composed of amino acids and uronic acid. It is a high molecular compound composed of N-acetylglucosamine and glucuronic acid. It is present in the vitreous body of the eye or the umbilical cord, and plays a role in preventing the penetration of bacteria or poisons. it is known On the other hand, hyaluronic acid is a polymer compound having a molecular weight of about 200,000 to 400,000 in which N-acetylglucosamine and glucuronic acid are alternately combined in a chain shape.

According to an embodiment of the present invention, centella extract, calamine and hyaluronic acid are selected from the group consisting of chitooligosaccharide, gelatin, pectin, polyethylene glycol, methyl cellulose, sodium carboxymethyl cellulose, hydroxymethylpropyl cellulose and ethyl cellulose. It may be nanoencapsulated with one or more polymers.

On the other hand, the nano-encapsulation method may be performed by a method generally used in the art, for example, may be performed by a nano precipitation method (Nano precipitation method). Specifically, the active ingredient and the hydrophobic polymer having a cationic functional group are dissolved in an appropriate organic solvent, that is, a solvent with high volatility that can be mixed with water and is non-toxic and has a lower vapor pressure than water, and then the aqueous phase and the organic phase are mixed with stirring at an appropriate speed. After preparing an emulsion by self-association, it can be prepared by evaporating the organic phase to prepare nanocapsules present in the aqueous phase.

On the other hand, the Centella extract may be obtained by extraction and fermentation from various organs or parts of Centella, for example, it may be a fermented product of Centella hot water extract obtained by fermenting a hot water extract obtained by hot water extraction of Centella into a specific strain. have. Specifically, the hot water extraction process may be performed by using distilled water or ethanol as a solvent, and extracting at a temperature of 50 to 100° C., more specifically 80 to 100° C. for 24 to 36 hours.

On the other hand, the fermented product according to an embodiment of the present invention is Lactobacillus buchneri , Lactobacillus paracasei subsp. tolerans , Lactobacillus harbinensis , Saccharomycopsis fibuligera and Pichia kudriavzevii may be naturally fermented with one or more strains selected from the group consisting of.

Specifically, when using the fermented Centella extract using the above strain, it is possible to exert an effect suitable for skin moisturizing or skin inflammation improvement use. Specifically, the strains may be cultured in YM medium to MRS medium under 35° C. temperature conditions for about 36 hours. On the other hand, the strain may be inoculated at 0.5 to 1% by weight based on the total weight of the Centella extract.

On the other hand, the fermentation process, after strain inoculation, may be carried out for 24 to 72 hours under a temperature condition of 25 to 33 ℃. Specifically, when fermented for less than 24 hours, there may be a problem that fermentation is difficult to be effectively performed, and if fermented for more than 72 hours, contamination may be a problem.

On the other hand, the fermented product obtained by the above method may be obtained by additionally performing a process of powdering through hot air drying through supernatant separation, supernatant filtration and sterilization processes.

On the other hand, the composition according to an embodiment of the present invention per 100 parts by weight of centella extract, calamine 30 to 60 parts by weight, hyaluronic acid 20 to 40 parts by weight, licorice extract 10 to 15 parts by weight, green tea extract 5 to 10 parts by weight , 3 to 8 parts by weight of the cypress extract, and 5 to 15 parts by weight of glycerin, and more specifically, may include 50 parts by weight of calamine and 20 parts by weight of hyaluronic acid per 100 parts by weight of the Centella extract.

In the present invention, licorice extract, green tea extract, and cypress extract may be included for skin irritation relief and skin soothing effect, and glycerin may be included to obtain additional skin moisturizing effect.

On the other hand, the composition according to one embodiment of the present invention may be one that has an antibacterial effect on Propionibacterium sludge tumefaciens arc Ness (Propionibacterium acnes, P.acnes) and Staphylococcus aureus (Staphylococcus aureus, S.aureus).

On the other hand, in one embodiment of the present invention provides a cosmetic composition for improving skin moisture or skin inflammation, containing the composition as an active ingredient.

On the other hand, the cosmetic composition may include components commonly used in cosmetic compositions in addition to the active ingredients such as the composition, and if necessary, oil, purified water, surfactant, humectant, lower alcohol, thickener, chelating agent, colorant, preservative And it may further include one or more components selected from the group consisting of fragrances.

Specifically, the cosmetic composition according to an embodiment of the present invention is a preservative, stabilizer, surfactant, thickener, solubilizer, humectant, emollient, UV absorber, preservative, bactericide, emulsifier, antioxidant, pH adjuster, organic and inorganic It may further include any one or more selected from pigments, fragrances, cooling agents, and limiting agents.

On the other hand, according to an embodiment of the present invention, the nanocapsule composition may be contained in an amount of 0.1 to 5% by weight based on the total weight of the cosmetic composition, and has an effect of moisturizing the skin and improving skin inflammation within the weight% range, If the weight % is less than the range, there may be a problem in that the skin moisturizing and skin inflammation improvement effect is insignificant.

On the other hand, when the nanocapsule composition is used as a cosmetic composition, the formulation may be appropriately selected according to the intended purpose, and may be prepared in formulations such as, for example, nutrient lotion, nutrient cream, essence, and emulsion. As an example, nutritional creams and emulsions are prepared to contain 5 to 20% by weight of oils (squalane, vegetable oil, petrolatum, etc.) in addition to the fermentation extract, and 3 to 15% by weight of wax components (stearyl alcohol, beeswax, etc.) In addition to the fermentation extract, the nutrient lotion and essence may be prepared to contain 5 to 30% by weight of polyhydric alcohols (glycerin, butylene glycol, etc.).

As described above, the nanocapsule composition for skin moisturizing or skin inflammation improvement according to the present invention has an effect of improving skin moisture or skin inflammation by improving skin moisture retention ability. In addition, propionyl sludge tumefaciens arc Ness (Propionibacterium acnes, P.acnes) and Staphylococcus aureus (Staphylococcus aureus, S.aureus) such as teenagers exposure excellent antibacterial effect on acne causing bacteria in diseases such as acne and skin troubles It can be usefully used as a cosmetic composition for

Meanwhile, according to one embodiment of the present invention, there is provided a quasi-drug composition for skin moisturizing or skin inflammation improvement containing the composition as an active ingredient. Like the above-described cosmetic composition, the quasi-drug composition may also have one or more uses selected from the group consisting of skin moisturizing enhancement, skin inflammation prevention and improvement.

The term "quasi-drug" used in the present invention refers to articles with a milder action than pharmaceuticals among articles used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals, for example, the Pharmaceutical Affairs Act. According to the Act, quasi-drugs exclude products used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans or animals, and products with minor or no direct action on the human body.

The quasi-drug composition is used for the purpose of moisturizing the skin or improving skin inflammation, more specifically, for the purpose of strengthening skin moisture, preventing skin troubles and improving acne, and there is no particular limitation in the formulation thereof, for example, a softening lotion, It may be a composition having the formulation of a nutrient lotion, massage cream, nutrient cream, pack, mask pack, mask sheet, gel or skin adhesion type cosmetic, and also a transdermal administration type such as lotion, ointment, gel, cream, patch or spray. It may be a composition having a formulation.

On the other hand, the quasi-drug composition may include, for example, thickeners, stabilizers, solubilizers, vitamins, conventional adjuvants such as pigments and fragrances, and carriers in addition to the fermented composition.

Since the present invention may have various changes and may have various forms, specific embodiments will be illustrated and described in detail below. However, this is not intended to limit the present invention to the specific disclosed form, it should be understood to include all modifications, equivalents and substitutes included in the spirit and scope of the present invention.

Preparation Example: Preparation of Centella Fermented Product

100 g of Centella was placed in a 2 L round-bottom flask, 1 L of distilled water was added, and refluxed at 90° C. for 24 hours to obtain a hot water extract, which was then cooled to room temperature and sterilized in a sterilizer. Next, the centella hot water extract was placed in a 2L Erlenmeyer flask, and pre-cultured Lactobacillus paracasei subsp. Tolerans were inoculated with 1 g based on 100 g of Centella hot water extract, then placed in a stirred incubator, and fermented at 35 ° C for 36 hours. After the fermentation, the fermentation residue was removed and filtered to obtain a supernatant, and sterilized using a sterilizer to obtain a fermented product.

Examples and Comparative Examples

A nanocapsule composition for skin moisturizing or skin inflammation improvement was prepared with the composition shown in Table 1 below (the volume of the nanocapsule was ignored).

(unit g) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Centella Extract 100 100 (unfermented) 100 (not encapsulated) 100 100 calamine 50 50 50 20 50
hyaluronic acid 20 20 20 20 10
betaine 5 5 5 5 5
beta glucan 5 5 5 5 5
Hydrolyzed Collagen 5 5 5 5 5 Galactomyces 5 5 5 5 5
Madecassoside 5 5 5 5 5
olive oil 2.5 2.5 2.5 2.5 2.5
PG 7 esters 2.5 2.5 2.5 2.5 2.5
arginine 2.5 2.5 2.5 2.5 2.5
Carbomer 2.5 2.5 2.5 2.5 2.5
xanthan gum 2.5 2.5 2.5 2.5 2.5
Licorice Extract 10 10 10 10 10
green tea extract 10 10 10 10 10
Cypress Extract 5 5 5 5 5
glycerin 10 10 10 10 10
Purified water 500 500 500 500 500

[Experiment 1. Measurement of skin moisturizing effect]

The electrical conductivity of the skin surface was measured using a Corneometer (Courage + Khazaka, GmbH, Germany), and the measurement temperature was 23° C., 50% relative humidity. The test materials prepared in the above-mentioned Examples and Comparative Examples were prepared in a constant temperature and humidity room. A certain amount was applied to the upper forearm of 20 healthy women (0.05/cm ² ), and skin moisture content was measured immediately after application, 1 hour after application, and 2 hours after application, and normal skin without any treatment was used as a control group. The experimental results are shown in Table 2 below.

immediately after application
skin electrical conductivity 1 hour after application
skin electrical conductivity 2 hours after application
skin electrical conductivity Example 1 80 78 74
Comparative Example 1 70 69 64
Comparative Example 2 73 65 62
Comparative Example 3 76 69 66
Comparative Example 4 74 68 67

Referring to the results of Table 2, in the case of the composition according to the embodiment of the present invention, it was confirmed to have excellent skin moisture retention and durability compared to Comparative Examples.

[Experiment 2. Antibacterial test]

After placing a paper disc of 8 mm on the medium inoculated with P. acnes , the extract was absorbed to be 3 mg/disc and dried sufficiently. P. acnes was an anaerobic bacteria, and gaspack system was used in anaerobic jar and incubated for 72 hours in an incubator at 37°C in a sealed state. After culturing, the clear zone including the disc diameter was measured.

Similarly, after placing an 8 mm paper disc on the medium inoculated with S. aureus , the extract was absorbed to 3 mg/disc, dried sufficiently, and cultured for 72 hours in an incubator at 37 ° C. After culturing, including the diameter of the disc The clear zone was measured.

The above experimental results are shown in Table 3 below.

P.acnes
clear zone (mm) S. aureus
clear zone (mm) Example 1 32.1 31.6
Comparative Example 1 28.5 26.5
Comparative Example 2 27.4 26.3
Comparative Example 3 25.3 24.3
Comparative Example 4 26.3 24.7

Referring to the results of Table 3 above, in the case of the composition according to an embodiment of the present invention, compared to Comparative Examples, Propionibacterium acnes , P. acnes, which is known as the causative bacteria of acne, and Staphylococcus aureus , S. aureus ), etc., can be confirmed to have excellent antibacterial effects, and it can be confirmed that superior acne and skin inflammation improvement effects can be expected compared to Comparative Examples.

In the foregoing, specific embodiments of the present invention have been described and shown, but it is common knowledge in the art that the present invention is not limited to the described embodiments, and that various modifications and variations can be made without departing from the spirit and scope of the present invention. It is self-evident to those who have Accordingly, such modifications or variations should not be individually understood from the technical spirit or point of view of the present invention, and modified embodiments should be said to belong to the claims of the present invention.

Claims (9)

Centella extract, calamine, hyaluronic acid, licorice extract, green tea extract, cypress extract, and nanocapsule composition for skin moisturizing or improving skin inflammation comprising glycerin.
The method of claim 1,
The centella extract, calamine and hyaluronic acid are nanoencapsulated with one or more polymers selected from the group consisting of chitooligosaccharide, gelatin, pectin, polyethylene glycol, methyl cellulose, sodium carboxymethyl cellulose, hydroxymethylpropyl cellulose and ethyl cellulose. Nanocapsule composition for skin moisturizing or skin inflammation improvement.
The method of claim 1,
The Centella extract is a fermented product of Centella hot water extract, a nanocapsule composition for skin moisturizing or skin inflammation improvement.
4. The method of claim 3,
The fermented product is Centella extract Lactobacillus buchneri , Lactobacillus paracasei subsp. tolerans , Lactobacillus harbinensis , Saccharomycopsis fibuligera and Pichia kudriavzevii , which is naturally fermented with one or more strains selected from the group consisting of, skin moisturizing or skin inflammation improvement nanocapsule composition.
The method of claim 1,
The composition comprises 30 to 60 parts by weight of calamine per 100 parts by weight of the Centella extract, 20 to 40 parts by weight of hyaluronic acid, 10 to 15 parts by weight of licorice extract, 5 to 10 parts by weight of green tea extract, 3 to 8 parts by weight of cypress extract, and A nanocapsule composition for skin moisturizing or skin inflammation improvement, comprising 5 to 15 parts by weight of glycerin.
The method of claim 1,
The composition propynyl sludge tumefaciens arc Ness (Propionibacterium acnes, P.acnes) and Staphylococcus aureus (Staphylococcus aureus, S.aureus) having an antibacterial effect on the skin moisture or nanocapsule composition for improving skin inflammation.
A cosmetic composition for skin moisturizing or skin inflammation improvement comprising the composition of claim 1 as an active ingredient.
8. The method of claim 7,
The cosmetic composition includes a preservative, a stabilizer, a surfactant, a thickener, a solubilizer, a moisturizer, an emollient, an ultraviolet absorber, a preservative, a disinfectant, an emulsifier, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cooling agent and a limiting agent. A cosmetic composition for improving skin moisture or skin inflammation further comprising one or more selected.
A quasi-drug composition for skin moisturizing or skin inflammation improvement comprising the composition of claim 1 as an active ingredient.

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