KR102343166B1 - Cosmetic composition having anti-oxidant, anti-inflammatory, skin soothing, regeneration, elasticity and moisturizing effects which comprise Melaleuca Alternifolia (Tea Tree) Leaf, Artemisia Princeps Leaf, Polygonum Fagopyrum (Buckwheat) Seed and Capryloyl Salicylic Acid - Google Patents

Abstract

The present invention relates to a cosmetic composition having antioxidant, anti-inflammatory, skin soothing, regeneration, elasticity, and moisturizing effects containing a mixing extract of a tea tree leaf, an artemisia princeps leaf, a buckwheat seed, and a capryloyl salicylic acid as active ingredients, and more specifically, to comprise a mixing extract of a tea tree leaf, an artemisia princeps leaf, a buckwheat seed, and a capryloyl salicylic acid as active ingredients. The mixing extract of the tea tree leaf, the artemisia princeps leaf, the buckwheat seed, and the capryloyl salicylic acid is extracted by mixing the tea tree leaf, the artemisia princeps leaf, the buckwheat seed, and the capryloyl salicylic acid in a weight ratio of 0.0001-40.0%, respectively. The cosmetic composition according to the present invention provides a skin antioxidant effect; provides the skin anti-inflammatory, skin soothing, regeneration, elasticity, and moisturizing effects; and provides no skin irritation and no side effects.

Description

Cosmetic composition having antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects containing tea tree leaves, mugwort, buckwheat and capryloyl salicylic acid {Cosmetic composition having anti-oxidant, anti-inflammatory, skin soothing, regeneration , elasticity and moisturizing effects which comprise Melaleuca Alternifolia (Tea Tree) Leaf, Artemisia Princeps Leaf, Polygonum Fagopyrum (Buckwheat) Seed and Capryloyl Salicylic Acid}

The present invention relates to a cosmetic composition containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract as an active ingredient to have antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects.

The skin of the human body is the largest organ among the organs constituting the body and is usually divided into the epidermis, dermis, and subcutaneous fat layer. Since the skin is always directly exposed to the external environment, it can receive various stimuli according to changes in the environment. As the outermost organ of the human body, the skin is responsible for the function of the first biological protective film, preventing moisture loss in the human body, and at the same time blocking the penetration of various harmful substances or contaminants that can be introduced from the outside, and protects the skin from UV rays. In a cold environment, it lowers heat loss through contraction, and in a hot environment, it plays an important role in maintaining the homeostasis of the human body by dissipating body heat by dissipating sweat.

Such skin is sensitive to severe fine dust, various pollutants, or unchanging external environmental factors such as sudden temperature changes, physical factors such as abrasions or strong ultraviolet rays from the sun, insect bites such as mosquitoes, bees and ticks, or biological factors such as contact with poison ivy sap. Due to various factors such as factors, bacterial infection, etc., abnormalities of the endocrine system or hypersensitivity of the skin immune system can cause skin problems, dryness, pruritus (itch), irritation, burning, inflammation, edema, etc. Various skin diseases can be expressed.

In order to protect the skin from factors that adversely affect the skin, the need for a cosmetic composition having antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects is increasing.

As a prior art related to the above problems, in Korean Patent Publication No. 10-2033088 (2019.10.17.), "Antibacterial, anti-inflammatory, antioxidant, sebum secretion inhibition and dermatitis improvement composition containing plant extract mixture" As disclosed, it relates to a composition for antibacterial, anti-inflammatory, antioxidant, sebum secretion inhibition and dermatitis improvement comprising a plant extract mixture, and more specifically, Prunella vulgaris, Magnolia liliflora, Glycine soya, It relates to a composition for antibacterial, anti-inflammatory, antioxidant, sebum secretion inhibition and dermatitis improvement comprising a mixture of five plant extracts of licorice (Glycyrrhiza glabra) and centella asiatica as an active ingredient.

However, the effect of the prior art was insignificant, and the need for a composition having a superior effect was growing.

Republic of Korea Patent Publication No. 10-2033088 (2019.10.17.), "Composition containing plant extract mixture for antibacterial, anti-inflammatory, antioxidant, sebum secretion inhibition and dermatitis improvement"

Accordingly, an object of the present invention relates to a cosmetic composition containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract as an active ingredient, having antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects.

In order to achieve the above object, the present invention is an antioxidant containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid, which contains a tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract as an active ingredient. , to provide a cosmetic composition having anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects.

In addition, the tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract is extracted by mixing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid in a weight ratio of 0.0001 to 40.0%, respectively. do.

In addition, the extract is characterized in that it is extracted using any one or a mixture of two or more extraction solvents selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin and ethylene glycol.

In addition, the cosmetic composition is a lotion, skin, lotion, cream, foundation, essence, gel, pack, emulsified sunscreen cream, emulsified foundation, emulsified makeup base, oil cake-type foundation, two-way cake, or powder pact. It is characterized in that it is formulated in a dosage form.

It is to provide antioxidant, anti-inflammatory, skin soothing, regeneration, elasticity and moisturizing effects containing the tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract according to the present invention as an active ingredient.

1 is a graph evaluating the cell viability for each sample treatment concentration according to the present invention.
2 is a graph evaluating the radical scavenging effect for each sample treatment concentration according to the present invention.
3 is a graph evaluating the inhibition of NO production by sample treatment concentration according to the present invention.
4 is a graph evaluating the skin soothing effect for each sample treatment according to the present invention.
5 is a graph evaluating the skin moisturizing effect for each sample treatment according to the present invention.
6 is a graph showing the amount of transdermal water loss for each sample treatment according to the present invention.
7 is a graph showing the skin elasticity improvement effect of the sample according to the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown by way of example of the embodiment. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the present invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included, not excluding other components, unless otherwise stated.

The present invention provides a cosmetic composition containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid as active ingredients to have antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects.

The "tea tree (Melaleuca Alternifolia (Tea Tree)") is an evergreen arboreal tree belonging to the genus oleifera family, and is a small shrub with linear leaves and white flowers. Tea tree contains various ingredients such as terpinen, cymene, cineol, pinene, and sabinene, and is known to have antioxidant, anti-inflammatory, antibacterial, skin regeneration, skin moisturizing and soothing effects.

The "wormwood (Artemisia Princeps Leaf)" is a perennial plant belonging to the Asteraceae (Compositae). It is known that medicinal mugwort has various uses depending on the part and time of year, and in particular, the stem and leaves are medicinal, young leaves are edible, and the general leaves are used to make moxa. Wormwood contains active ingredients such as acetylene, sesquiterpene lactone, eupatilin, caffeic acid, catechol, and protocatechunic acid. Based on these ingredients, mugwort extract is known to have antioxidant and anti-inflammatory properties.

The "buckwheat (Polygonum Fagopyrum (Buckwheat) Seed) is an annual plant belonging to the family Knobaceae and is classified taxonomically from wheat or cereals, but is included in the category of cereals. In particular, buckwheat contains a large amount of Rutin, so it has antioxidant, anti-inflammatory and It affects cell growth and exhibits typical physiological activities such as regeneration, etc. Also, buckwheat is known to be effective for skin atopy and moisturizing.

The "Capryloyl Salicylic Acid" is a derivative of salicylic acid developed by L'Oreal, and is made by bonding a fatty chain to the benzene ring of salicylic acid. With a pH of 5.5, it is almost similar to normal skin, so there is no need to neutralize it, and it is known to be excellent in exfoliation, sebum removal, antibacterial and anti-inflammatory effects by slowly penetrating the skin due to its oil-soluble nature.

In the present invention, tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid all have excellent antioxidant activity, and a mixture of at least two of the extracts, preferably tea tree leaf extract, mugwort extract, buckwheat extract and caprylo Ilsalicylic acid was found to have an excellent synergistic effect.

In particular, tea tree leaf extract, mugwort extract, buckwheat extract and capryloyl salicylic acid were used as active ingredients to impart antioxidant, anti-inflammatory, skin soothing, regeneration, elasticity and moisturizing effects.

According to a preferred embodiment of the present invention, the mixing ratio of the raw material extracted by mixing the present invention tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid may be included in the following ratio.

The tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract is preferably extracted by mixing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid in a weight ratio of 0.0001 to 40.0%, respectively. More specifically, the weight ratio of the tea tree leaf: mugwort: buckwheat: capryloyl salicylic acid may be 33.334: 33.334: 33.331: 0.001.

In the cosmetic composition having antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects containing tea tree leaf, mugwort, buckwheat, and capryloyl salicylic acid as active ingredients according to the present invention, the mixed extract is in the final form It contains at least 0.01 to 30.0% by weight, more preferably 0.1 to 20.0% by weight, and more preferably 1.0 to 10.0% by weight.

When the content of the mixed extract is less than 0.001% by weight, the antioxidant effect does not appear, and when it contains more than 30% by weight, the effect does not significantly increase, and the safety and stability of the formulation are problematic and economical efficiency is generally lowered.

The antioxidant cosmetic composition according to the present invention is a combination based on scientific basic data on skin. When using a mixed extract extracted by mixing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid as an active ingredient, antioxidant, anti-inflammatory, In terms of skin soothing, regeneration, elasticity, and moisturizing effects, it is possible to implement practically long-term efficacy.

The ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to the mixture extracted by mixing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid according to the present invention, for example, and conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments, pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, any one or more of animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as a carrier component can be used by selecting .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butyl Renglycol, 1,3-butyl glycol oil, polyoxyethylene hydrogenated castor oil, glycerol, glycerin, aliphatic ester, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleate, paraffin, Sorbitan stearate, lipophilic monostearic acid glycerin, stearic acid, glyceryl stearate/PEG-400 stearate, carboxypolymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, stearate-4, dicetylphosphate , macadamia oil, carboxyvinyl polymer, xanthan gum, or fatty acid ester of sorbitan may be selected and used.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol, glycerin, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Suspending agents such as microcrystalline cellulose, hydroxyethyl cellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate , sorbitan sesquioleate, glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate, wax, paraffin, squalane, caprylic/capric triglyceride, carboxyvinyl polymer, triethanolamine, agar or triglyceride Any one or more of Kant may be selected and used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Any one or more of ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be selected and used.

In addition, the efficacy of the cosmetic composition according to an embodiment of the present invention can be appropriately adjusted according to the individual's skin condition and condition, and external environmental conditions.

Hereinafter, to help the understanding of the present invention will be described in detail by presenting examples and experimental examples. However, the following examples and experimental examples are only provided for easier understanding of the present invention, and should not be construed as limiting the technical level and scope of the present invention thereby. The Examples and Experimental Examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

< Example 1 > tea tree leaves , mugwort, buckwheat and Capryloyl Salicylic Acid Preparation of mixed extract

Wash and dry each tea tree leaf, mugwort, and buckwheat in purified water, then pulverize the tea tree leaf, mugwort, and buckwheat: capryloyl salicylic acid in a ratio of 33.334: 33.334: 33.331: 0.001 to mix and pulverize 50 g/ Add 1 kg of aqueous solution to 30% ethanol so as to become L, and extract under reflux 3 times for 4 hours at 60 ~ 90 ° C., cooled to room temperature, filtered with Whatman #3 filter paper, and reduced pressure at 50 ° C. or less Concentrated and freeze-dried to prepare a mixed extract of tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid.

< Example 2 > tea tree leaves , mugwort and Capryloyl Salicylic Acid Preparation of mixed extract

Wash and dry each tea tree leaf and mugwort in purified water, then grind and mix tea tree leaf, mugwort and: capryloyl salicylic acid in a ratio of 49.9995: 49.995: 0.001 to make a mixed pulverized product of 50 g/L 30% 1 kg of aqueous solution was added to ethanol, refluxed and extracted 3 times for 4 hours at 60 ~ 90 ° C., cooled to room temperature, filtered with Whatman #3 filter paper, concentrated under reduced pressure at 50 ° C. or less, and freeze-dried. A mixed extract of tea tree leaf, mugwort and capryloyl salicylic acid was prepared.

< Example 3 > tea tree leaves , buckwheat and Capryloyl Salicylic Acid Preparation of mixed extract

Wash and dry each tea tree leaf and buckwheat in purified water, then pulverize the tea tree leaf, buckwheat and : capryloyl salicylic acid in a ratio of 49.9995 : 49.995 : 0.001 to make a mixed pulverized product of 50 g/L 30% 1 kg of aqueous solution was added to ethanol, refluxed and extracted 3 times for 4 hours at 60 ~ 90 ° C., cooled to room temperature, filtered with Whatman #3 filter paper, concentrated under reduced pressure at 50 ° C. or less, and freeze-dried. A mixed extract of tea tree leaf, buckwheat and capryloyl salicylic acid was prepared.

<Example 4> Preparation of mixed extract of mugwort, buckwheat and capryloyl salicylic acid

Mugwort and buckwheat are washed in purified water, dried, and then pulverized. Mugwort, buckwheat and: capryloyl salicylic acid are mixed in a ratio of 49.9995: 49.995: 0.001 to obtain 50 g/L of the mixed pulverized product, 1 aqueous solution in 30% ethanol. kg, extracted under reflux 3 times for 4 hours at 60 ~ 90℃ conditions, cooled to room temperature, filtered with Whatman #3 filter paper, concentrated under reduced pressure at 50℃ or less, and freeze-dried to produce mugwort, buckwheat and A mixed extract of capryloyl salicylic acid was prepared.

< production example and comparative example > skin soothing , regenerating, resilient and moisturizing effect Manufacture of active ingredients with

In order to evaluate the skin soothing, regeneration, elasticity and moisturizing effect ability, the tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixture of the present invention, glycerin (LG Household & Health Care), hyaluronic acid (same as Hobby Bio) known as conventional moisturizers Perm) was prepared as shown in Table 1, respectively, and the experiment was carried out.

Ingredient name Sample name Preparation Example 1 Preparation Example 2 Preparation 3 Preparation 4 Comparative Example 1 Comparative Example 2 A Preparation of tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract 2.0 Preparation of tea tree leaf, mugwort and capryloyl salicylic acid mixed extract 2.0 Preparation of tea tree leaf, buckwheat and capryloyl salicylic acid mixed extract 2.0 Preparation of Mugwort, Buckwheat and Capryloyl Salicylic Acid Mixed Extract 2.0 glycerin 2.0 Hyaluronic acid (0.5%) 2.0 EDTA-2NA 0.02 0.02 0.02 0.02 0.02 0.02 Purified water To 100 To 100 To 100 To 100 To 100 To 100 B Methylglucose sesquistearate 3.0 3.0 3.0 3.0 3.0 3.0 Glyceryl Stearate 0.8 0.8 0.8 0.8 0.8 0.8 Polysorbate 60 1.5 1.5 1.5 1.5 1.5 1.5 cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 beeswax 0.5 0.5 0.5 0.5 0.5 0.5 Caprylic/Capric Triglycerides 5.0 5.0 5.0 5.0 5.0 5.0 shea butter 0.7 0.7 0.7 0.7 0.7 0.7 dimethicone 2.0 2.0 2.0 2.0 2.0 2.0 tocopheryl acetate 0.3 0.3 0.3 0.3 0.3 0.3 fragrance, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

< test example 1 > Cell viability test

1) Experimental method

For the samples prepared in Examples 1 to 4, the effect on cell proliferation was confirmed. In order to confirm cell proliferation and toxicity, the experiment was carried out in the following manner.

Cells in culture 3T3 (fibroblast cell line) were dispensed in a 96-well microplate at 5,000 cells/ml, incubated in a thermostat for 30 minutes, and samples were incubated at each concentration of 0.0, 0.5, 1.0, 2.0, 3.0, 4.0. , 5.0 (%, V/V)) and cultured for 72 hours.

After 72 hours of incubation, Thiazoline blue was administered and further cultured for 4 hours. After discarding all of the culture solution, a reaction stop solution was added to each well of the microplate, stirred for 5 minutes, and absorbance was measured at 570 nm. The control group was co-cultured under optimal conditions for cell growth by administering 10% Fetal Bovine Serum (FBS) medium as much as the sample injection amount. did

The cell proliferation effect was calculated by Equation 1 below, and the results are shown in Table 2 below.

2) Experiment result

Sample name Cell viability (%) by sample treatment concentration (%) 0 0.5 1.0 2.0 3.0 4.0 5.0 Example 1 100 101.5 102.2 101.9 101.6 100.5 99.8 Example 2 100 101.1 101.9 102.3 102.1 101.3 100.1 Example 3 100 101.2 100.8 100.7 100.2 99.8 98.7 Example 4 100 101.1 100.8 100.3 99.4 98.9 97.4

As shown in Table 2, it was confirmed that none of the samples obtained by mixing extraction of tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid prepared as in Examples 1 to 4 were toxic.

<Test Example 2> Confirmation of free radical scavenging effect

1) Experimental method

For the samples prepared in Examples 1 to 4, the effect on free radical scavenging was confirmed. In order to confirm the effect on free radical scavenging, the experiment was conducted as follows.

Experiments were performed using the DPPH (1,1-diphenyl-2 picryl-hydrazyl) method (refer to: Blois. MSNature 181, 1190, 1958), DPPH and Quercetin were SIGMA's. . 2 ml each of Examples 1 to 4 and positive control of various concentrations (0.5, 1.0, 2.0, 3.0, 4.0, 5.0%) were added to 1 ml of 0.2 mM DPPH methanol solution, stirred, and reacted at room temperature for 10 minutes. Then, absorbance was measured at 517 nm, and purified water was used instead of each sample as a blank test.

The change of free radicals was measured using Equation 2 below, and the results are shown in Table 2.

2) Experiment result

The results for free radical scavenging are shown in Table 2 below.

Sample name By sample treatment concentration (%) Radical scavenging effect (%) 0.01 0.5 1.0 2.0 3.0 4.0 5.0 control 90.4 Example 1 28.3 45.0 55.6 67.5 73.6 76.2 Example 2 21.5 36.1 47.2 59.6 65.3 67.8 Example 3 20.9 34.8 45.1 56.7 64.8 66.7 Example 4 16.6 29.3 40.3 53.4 59.9 61.8

As shown in Table 3, it was confirmed that all of Examples 1 to 4 were effective in scavenging free radicals. In particular, in the case of Example 1, it was confirmed that the antioxidant effect was significantly higher than that of Examples 2 to 4.

<Test Example 3> Anti-inflammatory effect investigation by inhibition of NO production

1) Experimental method

Raw 264.7 cells, which are mouse macrophages, ^{were dispensed in a 6-well plate at a concentration of 3.5 Х 10 5} cells/ml and cultured for 24 hours. After that, each well was replaced with a cell medium containing diluted samples prepared in Examples 1 to 4. 1 μg of LPS (Lipopolysaccharide) was treated as a stimulus and cultured for 24 hours. 100 μl of the supernatant was taken and transferred to a 96-well plate, 100 μl of Griess solution was added and reacted at room temperature for 10 minutes, and the NO inhibitory effect was determined by measuring the absorbance at 540 nm, and the results are shown in Table 4 below. indicated.

Sample name By sample treatment concentration (%) NO production inhibition rate (%) 0.0 0.5 1.0 2.0 3.0 4.0 5.0 LPS + + + + + + + Example 1 0.0 6.67 15.83 20.83 27.50 34.17 37.50 Example 2 0.0 3.33 10.83 17.50 23.33 27.50 29.17 Example 3 0.0 0.83 9.17 13.33 18.33 24.17 27.50 Example 4 0.0 1.67 7.50 11.67 16.67 23.33 25.83

As shown in Table 4, it was confirmed that all of Examples 1 to 4 have the effect of inhibiting NO production in a concentration-dependent manner. In particular, in the case of Example 1, it was confirmed that the NO production inhibition rate was excellent as compared to Experimental Examples 2 to 4.

<Test Example 4> Soothing effect on skin irritants

The skin soothing effect was confirmed through the human skin intelligence test on 20 healthy adult men and women in their 20s and 50s who had no skin disease or allergy symptoms and had no history of hypersensitivity reactions.

To the cosmetics and control samples of Preparation Examples 1 to 4 and Comparative Examples 1 to 2, 5% of lactic acid, a skin irritant, was added, and a skin patch test was performed for each. Before proceeding with the sedation test, the inner part of the subject's forearm was thoroughly wiped with 70% ethanol, dried completely, and 30 ㎍ each of 9 cosmetics was applied. After 24 hours had elapsed based on the application time, the patch was removed, and after about 30 minutes, the patch site was observed with a magnifying glass (MicroView) to check for erythema and edema. The evaluation was performed based on the criteria of Table 5 below, and the average skin reactivity was calculated according to Equation 3 below. The results are shown in Table 6 below.

sign score skin reaction Evaluation standard - 0 non-irritant no response ± 0.5 unstimulated Faint or barely detectable mild erythema + One mild stimulation Demarcated but mild erythema ++ 2 central stimulus distinct erythema +++ 3 strong stimulus Severe erythema and blistering

2) Experiment result)

The results for the skin soothing effect are shown in Table 6 below.

Sample name score reactivity 0 0.5 1.0 2.0 3.0 Preparation Example 1 - 3 - - - 2.5 Preparation Example 2 - 4 - - - 3.3 Preparation 3 - 5 - - - 4.2 Preparation 4 - 4 - - - 3.3 Comparative Example 1 - 10 One - - 10.0 Comparative Example 2 - 11 2 - - 12.5 control sample - 14 3 - - 16.7

As a result of Table 6, Preparation Examples 1 to 4 were confirmed to have excellent skin soothing effects for stimulation induced through lactic acid when compared to control samples, whereas Comparative Examples 1 to 2 were compared for stimulation induced by the case It was confirmed that there was no significant difference from the sample.

In addition, comparing Preparation Examples 1 and 2 to 4, it was confirmed that the synergistic effect was the most excellent in Preparation Example 1, in which tea tree leaf, mugwort, buckwheat, and capryloyl salicylic acid were mixed.

<Test Example 5> Cell regeneration ability measurement

1) Experimental method

HACAT keratinocytes were dispensed in a 24-well plate at ^{a concentration of 1.0 Х 10 5} cells/ml and cultured for 24 hours, and then the cells were scraped off with a 200 μl pipette tip. Then, tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract 2% was added to the well plate and cultured for 72 hours to measure the degree of cell migration. Cell migration ability was confirmed using EFG as a control.

2) Experiment result)

The experimental results confirmed the cell regeneration effect as shown in Table 7.

hour Control (EGF) Example 1 Example 2 Example 3 Example 4

0

72

As shown in Table 7, in all of Examples 1 to 4, regeneration ability through cell migration was confirmed at a sample concentration of 2%. In particular, in the case of Example 1, it was confirmed that the level of EGF, which is a control, showed cell regeneration ability, and thus had superior regeneration ability than Examples 2 to 4.

<Test Example 6> skin moisturizing effect

The cosmetics prepared in Preparation Examples and Comparative Examples of Table 1 were applied to 20 subjects (male and female of 25-50 years of age) twice a day for 4 weeks on both sides of the face and in the first half of Preparation Examples 1 to The cosmetic formulations of 4 and Comparative Examples 1 and 2 were respectively applied and the skin moisture improvement status was measured before, after 2 weeks, and after 4 weeks using a skin moisture meter Corneometer CM 820 (Courage + Khazaka, Germany). The unit is expressed as an arbitrary unit (Arbitrary Unit, AU) given by the device, and the higher the measured value, the higher the moisture content on the skin surface.

2) Experiment result

The experimental results on skin moisture improvement are shown in Table 8 below.

Sample name Skin moisturizing effect (AU) before application 2 weeks later after 4 weeks Preparation Example 1 54.6 59.8 68.8 Preparation 2 53.7 58.8 66.9 Preparation 3 54.4 58.7 66.3 Preparation 4 53.9 58.4 65.9 Comparative Example 1 55.1 54.9 55.3 Comparative Example 2 54.7 54.8 55.1

As shown in Table 8, the moisturizing effect was measured for the cosmetic compositions prepared according to Preparation Examples 1 to 4 and Comparative Examples 1 to 2, and as a result, the moisturizing effect was higher in Preparation Examples 1 to 4 than Comparative Examples 1 to 2 could check In particular, in the case of Preparation Example 1, it was confirmed that the moisturizing effect was more excellently increased than in Preparation Examples 2 to 4.

< test example 7 > transdermal water loss Confirm

1) Experimental method

For each of the cosmetic compositions of Preparation Examples 1 to 7 and Comparative Examples 1 and 2 in Table 1, 20 subjects (male and female in their 20s to 50s) set the upper arm as the measurement site, and set the width and length 2 Х 2 cm After marking the test site with a size of ^{2, measurements were made.} After applying the cosmetic composition to the test site, it was rubbed well, and the amount of transdermal moisture loss was measured using TEWAMETER TM210 (C+K eletronic GmhH. Germany). Short-term moisturizing effect was evaluated by measuring the moisture increase rate (%) 30 minutes after application, 4 days after application, and 8 days after application, based on the water retention amount of 0 hours before application. During the measurement, the amount of transdermal water loss was measured three times consecutively while keeping the probe vertical for 60 seconds. Measurements were carried out in the same temperature and humidity room, and the higher the value, the greater the loss of moisture.

2) Experiment result

The skin moisturizing results for the amount of percutaneous moisture loss are shown in Table 9 below.

Sample name Water loss (%) 30 minutes after application 1 day after application 4 days after application 8 days after application Preparation Example 1 26.5 25.3 22.7 19.6 Preparation Example 2 26.8 25.9 23.9 21.8 Preparation 3 27.1 26.3 24.4 22.3 Preparation 4 27.2 26.5 24.6 22.8 Comparative Example 1 34.5 34.3 33.9 33.4 Comparative Example 2 37.2 37.0 36.6 36.3

As shown in Table 9, as a result of comparing the amount of transdermal water loss in Preparation Examples 1 to 4 and Comparative Examples 1 to 2 for 8 days, it was confirmed that the amount of water loss was reduced in Preparation Examples 1 to 4 compared to Comparative Examples 1 and 2. In particular, it was confirmed that Preparation Example 1 had the highest loss amount improvement than Preparation Examples 2 to 4.

<Test Example 8> Confirmation of skin elasticity improvement effect

The skin elasticity improvement effect was evaluated by performing a skin elasticity improvement effect test on the samples prepared in Preparation Examples 1 to 4 and Comparative Examples 1 to 2 on humans.

The cosmetics used in the comparative experiment were samples prepared in Preparation Examples 1 to 4 and Comparative Examples 1 to 2. The samples prepared in Preparation Examples 1 to 4 and Comparative Examples 1 to 2 were applied to 20 experimenters (females aged 20-35 years) for each part of the face twice a day for 2 consecutive months.

After completion of the experiment, the skin elasticity improvement effect was measured using a skin elasticity meter (cutometer SEM 575, C+K Electronic Co., Germany) before and after using the product for 2 months.

Experimental results are described in Table 10 below as ΔR7 values of Cutometer SEM 575, and R7 values indicate viscoelasticity of the skin. As shown in Table 10, it can be seen that when Preparation Examples 1 to 4 were applied, the skin elasticity improvement effect was excellent.

Skin elasticity improvement effect (△R7) Preparation Example 1 0.38 Preparation Example 2 0.35 Preparation 3 0.34 Preparation 4 0.33 Comparative Example 1 0.22 Comparative Example 2 0.24

Hereinafter, based on the results of the above experimental examples, a cosmetic composition containing a tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract is formulated and presented as follows. However, the composition of the present invention is not limited to the following formulation examples.

<Formulation Example 1> Preparation of toner containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract as an active ingredient

Example 1 Table 11 shows toner formulation examples in cosmetics containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract.

number Raw material content (wt%) Raw material content ratio (%) One Tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract 2.00 2 glycerin 5.00 3 methyl propanediol 3.00 4 betaine 1.00 5 Polyglyceryl-10 Oleate 0.50 44% Polyglyceryl-10 Stearate 56% 6 1,2-Hexanediol 1.20 7 Disodium EDIT 0.02 8 antiseptic a very small amount 9 Spices a very small amount 10 Purified water remaining amount

<Formulation Example 2> Preparation of lotion containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract as an active ingredient

Example 1 A formulation example of a lotion in a cosmetic containing a tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract is shown in Table 12.

number Raw material content (wt%) Raw material content ratio (%) One Tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract 2.00 2 Caprylic/Capric Triglycerides 5.00 3 cetearyl alcohol 1.00 4 Stearic Acid 0.50 5 glycerin 5.00 6 methyl propanediol 3.00 7 propanediol 3.00 8 dimethicone 0.50 9 Cyclopentasiloxane 1.00 65% Cyclohexasiloxane 35% 10 cetearyl olivate 1.00 50-70% Sorbitan Oliveate 35-50% 11 Polyglyceryl-3 methylglucose distearate 1.00 12 1,2-Hexanediol 1.20 13 Glyceryl Stearate 0.50 14 Polysorbate 60 0.30 15 tromethamine 0.18 16 Disodium EDIT 0.02 17 Ammonium acryloyldimethyl taurate/vpicopolymer 0.10 18 Sodium Polyacrylate 0.10 55-65% Hydrogenated Polydecene 30-40% Trideces-6 < 5% 19 Carbomer 0.10 20 xanthan gum 0.05 21 antiseptic a very small amount 22 Spices a very small amount 23 Purified water remaining amount

<Formulation Example 3> Preparation of cream containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract as an active ingredient

Example 1 A formulation example of a cream in a cosmetic containing a tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract is shown in Table 13.

number Raw material content (wt%) Raw material content ratio (%) One Tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid mixed extract 2.00 2 Caprylic/Capric Triglycerides 7.00 3 Dicaprylyl Carbonate 3.00 4 cetearyl alcohol 2.00 5 Stearic Acid 0.20 6 glycerin 5.00 7 propanediol 5.00 8 dimethicone 0.50 9 Cyclopentasiloxane 1.00 65% Cyclohexasiloxane 35% 10 1,2-Hexanediol 1.20 11 Trehalose 0.50 12 Glyceryl Stearate Citrate 3.00 13 Glyceryl Stearate 0.30 14 Polysorbate 60 0.30 15 tromethamine 0.18 16 Disodium EDIT 0.02 17 Ammonium acryloyldimethyl taurate/vpicopolymer 0.10 18 Sodium Polyacrylate 0.40 55-65% Hydrogenated Polydecene 30-40% Trideces-6 < 5% 19 Carbomer 0.20 20 xanthan gum 0.05 21 Sodium Hyaluronate 0.005 22 antiseptic a very small amount 23 Spices a very small amount 24 Purified water remaining amount

Although the present invention has been described in conjunction with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent to that by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality for more clearly explaining the configuration.

Claims (4)

Tea tree leaf, mugwort, buckwheat, and capryloyl salicylic acid were mixed in a weight ratio of 0.0001 to 40.0%, respectively, an extraction solvent was added, extracted under reflux 3 times at 60 to 90 ° C. for 4 hours, and cooled to room temperature. A cosmetic composition having antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid, which is a mixed extract concentrated under reduced pressure and freeze-dried by filtration, as an active ingredient .
delete The method of claim 1,
The extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, and tea tree leaf, characterized in that the extraction is performed using any one or a mixture of two or more extraction solvents selected from the group consisting of ethylene glycol , mugwort, buckwheat and capryloyl salicylic acid, a cosmetic composition having antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects.
The method of claim 1,
The cosmetic composition is in the form of a lotion, skin, lotion, cream, foundation, essence, gel, pack, emulsified sunscreen cream, emulsified foundation, emulsified makeup base, oil cake-type foundation, two-way cake, or powder pact. A cosmetic composition having antioxidant, anti-inflammatory, skin soothing, regenerating, elastic and moisturizing effects containing tea tree leaf, mugwort, buckwheat and capryloyl salicylic acid, characterized in that it is formulated.

Images