KR101574248B1 - Cosmetic composition comprising Reynoutria sachalinensis extracts for skin anti-wrinkle effect - Google Patents

Abstract

The present invention provides a cosmetic composition for improving skin wrinkles comprising an extract of Reynoutria sachalinensis as an active ingredient. The cosmetic composition for improving skin wrinkles containing the royal palm extract of the present invention as an active ingredient is excellent in ultraviolet ray restoration effect and inhibits MMP-1 collagen degrading enzyme which causes wrinkles caused by ultraviolet rays in a concentration-dependent manner, Provided is a cosmetic composition for improving skin wrinkles which shows an effect of improving skin wrinkles when the composition is added to a cosmetic base.

Description

[0001] The present invention relates to a cosmetic composition for improving skin wrinkles containing an extract of royal palm leaves as an active ingredient. [0002] Cosmetic composition comprising Reynoutria sachalinensis extracts for skin anti-

The present invention relates to a cosmetic composition for improving wrinkles containing an extract of royal jelly, and more particularly to a cosmetic composition for cosmetic treatment which contains MMP-1 collagen degrading enzyme which is excellent in ultraviolet ray recovery effect and causes wrinkle formation by ultraviolet rays, And to provide a cosmetic composition for improving skin wrinkles, which contains, as an active ingredient, a royal palm extract which exhibits an effect of improving skin wrinkles when the cosmetic composition is added to a cosmetic base.

Aging is an indispensable part of life. All creatures, whether animals or plants, are born on the path of aging. Thanks to hygiene and health care, human longevity has been significantly extended over the last century. As a result, the proportion of elderly people is steadily increasing. The process of aging takes place in the whole body of the body, with changes in the physiological functions of organs, such as hypertension, chronic coronary artery disease or diabetes, as well as aging. Skin aging is also progressing with age.

Skin aging can be broadly categorized as a photoaging that appears on the face, neck, and back of a person by continuous exposure to environmental factors such as intrinsic aging and sunshine, which can be seen over time by biological processes. Both endogenous aging and photoaging are caused by the accumulation of damage to the constituents of our body caused by harmful free radicals, but the photoaging is different in that this active oxygen is produced by ultraviolet rays.

Ultraviolet rays are the main cause of skin aging and are divided into UVA (320 ~ 400nm), UVB (280 ~ 320nm) and UVC (200 ~ 280nm) depending on the wavelength. The wavelengths are UVA and UVB. Among them, UVA penetrates deeply into the dermis, which is the major cause of damaging fibroblasts that produce collagen that maintains skin elasticity. It activates free radicals in the skin, destroying the antioxidant defense system in vivo and damaging cells and tissues At this time, lipids, proteins, and polysaccharides, which are major constituents of the skin, are oxidized. Among them, the oxidation of proteins breaks down the connective tissues like collagen and, in severe cases, it induces cancer or triggers immunosuppression. Activation of free radicals in vivo accelerates the degradation of extracellular matrix by increasing matrix metalloprotease (MMP), an enzyme that degrades collagen by reactive oxygen species, resulting in reduced skin elasticity and wrinkles .

Therefore, extensive efforts have been made to inhibit wrinkle formation, and there have been studies on new natural substances which can replace EGCG (epigallocatechin gallate), retinoid and derivatives thereof as a typical inhibitor of collagen decomposition, . Therefore, in the past, chemical cosmetics were popular, but in recent years, due to the arrival of the era of well-being, interest in the skin has been amplified so that functional cosmetics using various plant extracts that are hypoallergenic and expected to have efficacy as cosmetics Cosmeceuticals) are being manufactured.

As a conventional patent related to a cosmetic composition using a plant extract that is hypoallergenic and exhibits wrinkle-reducing effect to replace chemical ingredients having skin irritation as described above, Korean Patent Registration No. 10-0429590 entitled " Korean Patent Registration No. 10-0670238 entitled " Cosmetic Composition for Improving Skin Wrinkles Containing a Herbal Extract and Its Preparation Method ", Korean Patent Laid-Open Publication No. 10-2007-0111025 " And Korean Patent Registration No. 10-1009766 entitled " Cosmetic Composition for Wrinkle Reduction ", which contains the extracts of Chrysomelus edulis and Aspergillus oryzae, and the like. However, cosmetic compositions containing Reynoutria sachalinensis extract as a main component have not existed yet.

Korean Patent Registration No. 10-0429590 Korean Patent Registration No. 10-0670238 Korean Patent Publication No. 10-2007-0111025 Korean Patent Registration No. 10-1009766

The present inventors have made extensive efforts to develop a skin wrinkle-improving cosmetic material having excellent skin wrinkle-improving effect by using various natural plant extracts. As a result, when the extract of Reynoutria sachalinensis was used, the effect of improving the survival rate of the fibroblasts by ultraviolet , And inhibits MMP-1 collagenase in a concentration-dependent manner. When the cosmetic composition is added to a cosmetic base, it has an effect of improving skin wrinkles, thereby completing the present invention.

Accordingly, an object of the present invention is to provide a royal palm extract which exhibits an effect of improving skin wrinkles.

Another object of the present invention is to provide a cosmetic composition for improving skin wrinkles containing the above-described Wang Ho-gang extract.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

The present invention provides a cosmetic composition for improving skin wrinkles comprising an extract of Reynoutria sachalinensis as an active ingredient.

The royal roots are selected from the group comprising ground, flower, leaf, stem, root, fruit, seed and top.

The Wangwoongjang extract is an extract obtained by extracting with water, an organic solvent or a mixture thereof.

The organic solvent is at least one organic solvent selected from a hydrocarbon compound having 1 to 30 carbon atoms, a hydrocarbon derivative, or a mixture thereof.

The extract is extracted using at least one extraction method selected from the group consisting of heating at 45 to 120 ° C for 30 minutes to 24 hours followed by warming extraction, or 5 to 40 ° C for 30 minutes to 15 days for shaking extraction.

The royal palm extract contains 0.01 to 30% by weight based on the total weight of the skin wrinkle-improving cosmetic composition.

The Wangwoongjang extract has ultraviolet light recovery effect and MMP-1 collagenase inhibitory effect.

The cosmetic composition is any one of softening longevity, nutritional lotion, eye cream, essence, nutritional cream, massage cream, body cream, cleansing cream, cleansing foam, cleansing water, powder and pack.

The extract of Reynoutria sachalinensis of the present invention is excellent in ultraviolet ray restoration effect and inhibits MMP-1 collagenase which is a cause of wrinkle formation due to ultraviolet rays in a concentration-dependent manner, and thus has excellent wrinkle-reducing effect. In addition, when the Wang Hocho extract is added to a cosmetic base, the skin elasticity tends to increase drastically, and the area of wrinkles also decreases with time, and the effect as a cosmetic composition for improving skin wrinkles is excellent.

FIG. 1 is a graph showing the results of measurement of MMP-1 inhibitory activity of Wang Hojang Extract.

The present invention provides a cosmetic composition for improving skin wrinkles containing an extract of Reynoutria sachalinensis as an active ingredient.

In the present invention, the term 'extract' refers to an extract obtained by extracting Reynoutria sachalinensis using at least one extraction method selected from the group consisting of warm extraction using a solvent or extraction with an organic solvent, followed by filtration to obtain an extract Or by filtration, followed by concentration with a rotary vacuum evaporator to thereby maximally evaporate the solvent, followed by lyophilization.

Reynoutria sachalinensis is a perennial herb that grows in the valleys of mountainous terraces. Stems are straight and 2-3 meters high, columnar, hollow and green, but sunny parts are reddish. The young lady looked like bamboo shoots. Purple spots are spread in the shape of a hoof on the young stem. Leaves are alternate, triangular ovate, bigger than ovary, and white on back. The stem is thick and widespread, brown on the outside and yellow on the inside. The flowers are dioecious, and flowers are unicellular in August ~ September, blooming on the axil and end of branch. The fruit bear fruit (果果). Rootstock is used as a diuretic and general economy, and it is distributed in Korea's Ulleungdo, Japan, and Sakhalin in Russia. It is similar to Hojangguk on the surface, but it is bigger, the lower part of the leaf looks like the bottom of the heart, and the white color on the back side is different.

Reynoutria sachalinensis is used for the treatment of menstrual occlusive pain and for the treatment of limb paralysis and bruises, which are caused by stagnation in the meridians. It is also applied to jaundice and gallbladder stones caused by accumulation of moist heat. It is applied to a rash where the skin or boil has been torn down. When it is used as a snake or fire or hot water for external use, it is applied to the affected part. In addition, it is used for hemorrhagic hemorrhage or traumatic hemorrhage due to hemostasis, and it is known that it has antimicrobial, antiviral action, and secretory action, which is used for treating fever, controlling constipation, and treating P. aeruginosa infection. But there is no report that it can be used as a wrinkle-improving cosmetic product yet. Therefore, it has been confirmed that Wang Hojang extract is superior in ultraviolet ray recovery and collagenase inhibitory effect during the research on the above plants.

According to a preferred embodiment of the present invention, the royal roots can be selected from the group consisting of ground parts, flowers, leaves, stalks, roots, fruit, seeds and tops.

According to a preferred embodiment of the present invention, the Wang arborization is carried out by heating the Wang arbor under an extraction solvent selected from water, an organic solvent or a mixture thereof, heating at 45 to 120 ° C for 30 minutes to 24 hours, heating at 5 to 40 ° C It is preferable to use at least one extraction method selected from the methods of immersion for 30 minutes to 15 days and shaking extraction in view of improving the yield of the extract. The organic solvent may be at least one organic solvent selected from a hydrocarbon compound having 1 to 30 carbon atoms, a hydrocarbon derivative, or a mixture thereof. Although the weight of the extraction solvent is not particularly limited, it is difficult to extract the extract sufficiently if the amount is too small compared to the royal palm plant, and when it is used in excess, the extraction solvent is removed and the time and cost . In this case, it is necessary to sufficiently extract the sample from the warm-up when the warm-up extraction is carried out, and to perform extraction in a warmed state in order to increase the extraction efficiency. If the temperature is too low, it is difficult to sufficiently take advantage of the warm- If the temperature is too high, decomposition of the useful substance may occur. In case of shaking, it can be extracted at room temperature. The sample in powder form is immersed in a sufficient extraction solvent for a sufficient time, for example, about 3 days, so that the extraction can be sufficiently performed. If the extraction is not sufficient, Or increase the extraction volume by increasing the extraction time.

The royal roots extracted from the above-mentioned method may contain 0.01 to 30% by weight, more preferably 0.01 to 10% by weight based on the total weight of the cosmetic composition. If the extract is less than 0.01% by weight based on the total weight of the cosmetic composition, the effect of improving skin wrinkles can not be sufficiently obtained, so that the function as a skin wrinkle improving cosmetic is deteriorated. When it exceeds 30% by weight, It is uneconomical because it can exhibit sufficient efficacy even when it is not contained.

The extract of Wang Hojang prepared from the above method has a remarkable effect of restoring ultraviolet light and inhibiting MMP-1 collagenase and exhibiting an excellent wrinkle-reducing effect. When the Wang Houchang extract is added to a cosmetic base, skin elasticity is dramatically increased , And the area of the wrinkles also decreases with time, thereby improving the wrinkles of the skin.

As described above, the cosmetic composition for improving the skin wrinkles containing the extract of royal jelly can be used as a cosmetic composition for softening the skin, Pack. ≪ / RTI >

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

Example

Example 1: Preparation of an extract using water as an extraction solvent

Ryegrass ( Reynoutria sachalinensis ) was prepared as the outpost, ground, flower, leaf, stem, root, fruit and seed samples respectively. Next, the above-mentioned Wang Ho-kang sample was put into purified water having a weight ratio of 5 times and heated at 105 ° C for 5 hours. When the extraction process is completed, it is filtered with Whatman paper No. 1, re-filtered through a 0.45 μm membrane filter (TF-450, 60173, PALL Life Sciences), and then firstly washed with a rotary vacuum evaporator Followed by concentration with a rotary vacuum evaporator followed by lyophilization. As a result, 9.21 g of the extract of Wang Ho-jang extract, 8.07 g of the above-ground extract, 8.66 g of the flower extract, 10.13 g of the leaf extract, 9.24 g of the stem extract, 7.87 g of the root extract, 8.80 g of the fruit extract and 7.19 g of the seed extract were obtained.

Example 2: Preparation of an extract using methanol as an extraction solvent

Ryegrass ( Reynoutria sachalinensis ) was prepared as the outpost, ground, flower, leaf, stem, root, fruit and seed samples respectively. Next, three times of extracting solvent (methanol) was added to 0.1 kg of the Wang Hojo sample, and the mixture was shaken for 72 hours at 25 ° C. The extract was filtered through filter paper and filtered through a 0.45 μm membrane filter The mixture was filtered through a rotary vacuum evaporator and lyophilized to obtain 8.11 g of Wang Ho-jang extract, 7.46 g of the above-ground extract, 7.01 g of flower extract, 8.76g of leaf extract, 8.98g of stem extract, 7.09g of root extract, 8.11g of fruit extract and 8.26g of seed extract were obtained.

Example 3: Preparation of an extract using hexane as an extraction solvent

Ryegrass ( Reynoutria sachalinensis ) was prepared as the outpost, ground, flower, leaf, stem, root, fruit and seed samples respectively. Next, a step of adding 50-fold extraction solvent (hexane) to 0.1 kg of the Wang Ho-kang sample and shaking extraction at 25 ° C for 5 days was performed once. The extract was filtered with a filter paper and filtered through a 0.45 μm membrane filter The mixture was concentrated by using a rotary vacuum evaporator and lyophilized to obtain 10.87 g of Wang Ho-jang extract, 10.55 g of the above-ground extract, 11.19 g of flower extract, 10.56g of leaf extract, 9.13g of stem extract, 9.12g of root extract, 9.01g of fruit extract and 8.43g of seed extract were obtained.

Example 4: Preparation of an extract using butylene glycol as an extraction solvent

Ryegrass ( Reynoutria sachalinensis ) was prepared as the outpost, ground, flower, leaf, stem, root, fruit and seed samples respectively. Then, the extract solution was filtered through filter paper and membrane filter (TF-450, 60173, manufactured by Kagaku Kogyo Co., Ltd.) (Pall Life Sciences), 0.711 kg of solid extract (1.8% of solid content), 0.816 kg of solid extract (1.8% of solid content), 0.755 kg of flower extract (1.7% of solids content) (Solid content: about 2.0%), stem extract 0.955kg (solid content 2.2%), root extract 0.851kg (solid content about 3.3%), fruit extract 0.757kg (solid content about 3.2%), seed extract 0.981kg 1.4%).

Experimental Example 1: Ultraviolet light-recovering effect 1

The ultraviolet ray restoration effect of the royal roots extract obtained by the methods of Examples 1 to 4 was measured. Human fibroblasts were cultured in a CO2 incubator at 37 < 0 > C in order to examine the cytotoxicity of the extracts of the present invention. Thereafter, the culture medium was removed, washed with PBS (phosphate buffered saline), irradiated with ultraviolet rays using a UV irradiator system with PBS, treated with the respective extracts at the respective concentrations, Lt; / RTI > After incubation, MTT {3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide} solution was added to the wells and incubated at 37 ° C. After the culture was removed, DMSO (dimethyl sulfoxide) was added, and the resultant was shaken appropriately. The absorbance was measured using a microplate reader. Ascorbic acid was used as a positive control for the comparison of test results with a concentration of 100 μg / ml.

The results of the ultraviolet light recovery effect are shown in Table 1 below.

Samples (100 mu g / ml) UVA Positive control Example 1 Example 2 Example 3 Example 4 Ultraviolet light recovery effect
(%) outpost 56 68 85 91 72 78 Above ground 56 68 88 86 90 75 Flower 56 68 63 60 54 56 leaf 56 68 70 85 87 62 stem 56 68 75 90 93 90 Root 56 68 73 96 71 92 Fruit 56 68 86 97 75 80 strain 56 68 75 81 66 82

As a result, as shown in Table 1, the cytotoxicity of Example 2 showing the best ultraviolet ray restoration effect was selected.

Experimental Example 2: Cytotoxicity test (Neutral red assay)

The cytotoxicity of the extract of Wang Hocho obtained by the method of Example 2 was measured. In order to examine the cytotoxicity of the extract of Wang Hojang according to the present invention, human fibroblasts were cultured in a CO2 incubator at 37 ° C. After culturing, the Wangwoongjang extract was treated at different concentrations and further cultured under the same culture conditions. After the incubation, the MTT solution was added and the culture solution was removed. Then, the culture solution was removed, and DMSO (dimethyl sulfoxide) was added thereto. After shaking, the absorbance was measured using a microplate reader. .

Here, the control in the cytotoxicity test means a blank in which the above extract is not added.

Samples (占 퐂 / ml) part Control One 5 10 25 50 75 100 Example 2
Growth rate (%) outpost 100 99.00 96.87 95.13 90.25 - - - Above ground 100 98.46 100.07 96.31 94.09 - - - Flower 100 103.33 99.94 100.62 94.11 89.38 - - leaf 100 101.01 97.70 96.65 92.78 86.57 - - stem 100 105.52 104.51 100.77 96.34 - - - Root 100 109.75 104.05 95.92 - - - - Fruit 100 98.66 95.12 93.98 91.23 - - - strain 100 99.46 97.97 95.08 - - - -

* -: Indicates cytotoxicity

As a result, as shown in Table 2, it was confirmed that when the cells were treated with a concentration of 25 μg / ml or less in the Wangjangjang, the top, stem, and the fruit extract of Example 2, they did not show any toxicity. In addition, it was confirmed that 50 ㎍ / ㎖ was not detected in Wangjangjang flower and leaf extract, and 10 ㎍ / ㎖ in Wangjangjang root and seed extract.

Experimental Example 3: Ultraviolet light recovery effect 2

As shown in Table 2, the ultraviolet ray-recovering effect according to the concentration of the Wang Ho-jang extract was measured by the same method as Experimental Example 1 based on the maximum concentration that does not exhibit toxicity, and the results are shown in Table 3.

Samples (占 퐂 / ml) UVA Control 50 25 10 5 One character
outside
line
time
complex
Hyo
Ability
(%) outpost 51 60 - 63 61 55 47 Above ground 51 60 - 67 60 52 46 Flower 51 60 59 60 49 46 50 leaf 51 60 70 62 59 50 52 stem 51 60 - 53 54 50 43 Root 51 60 - - 73 66 62 Fruit 51 60 - 94 83 60 51 strain 51 60 - - 70 64 53

* -: Indicates cytotoxicity

As a result, as shown in Table 2, in the case of the Wang Hojang Fruit Extract of Example 2, it was found that the ultraviolet light recovery effect was remarkably improved by significantly increasing the concentration. In the case of ascorbic acid (100 μg / ml) as a control, the activity was 60%.

Experimental Example 4: Measurement of inhibitory effect on MMP-1

In order to investigate the effect of the fruit of Wang Ho-Gang, the best efficacy in Table 3, on collagen production, the effect of the extract on MMP-1 inhibition was examined using a Western blot method. Human fibroblasts were cultured in an incubator. After the incubation, the culture medium was removed, washed with PBS (phosphate buffered saline), and then UV-irradiated using a UV irradiator system. Thereafter, the extracts were treated at different concentrations and cultured under the same culture conditions. Cells were collected and centrifuged to remove the supernatant. Suspended in a pellet with Pro-PREPTM Solution (Intron), centrifuged again at high speed, and the supernatant was collected to obtain the protein of the cells. Next, the sample was electrophoresed with the marker to perform Western blotting. After completion, the gel was separated and the protein was transferred to the membrane. After blocking, treatment with MMP-1 (Matrix metalloproteinase 1) (R & D Systems) and Actin (Santacruz) primary antibody and TBS buffered saline Washing was carried out. After washing, the secondary antibody was treated and the protein band was identified using a chemiluminescent horseradish peroxidase substrate (Immobilon, Millipore) using Chemidoc (Atto corporation) instrument and CS Analyzer (Atto corporation) image analysis software.

For comparison of test results, EGCG (Epigallocatechin gallate) at a concentration of 10 μM was used as a positive control.

The results of measuring the MMP-1 inhibitory effect of the Wangjiaojiang extract are shown in Fig. 1 and Table 4.

Samples (占 퐂 / ml) Control Example 2 (Wang Hojang Fruit Extract) 25 10 5 MMP-1 inhibition activity (%) 40 72 65 47

As a result, as shown in FIG. 1 and Table 4, the Wang Hojang Fruit Extract of Example 2 showed a significance to inhibit MMP-1 collagenase, which causes wrinkle formation by ultraviolet rays, in a concentration-dependent manner, It was found that the effect of improving wrinkles was much better than that of control.

The following shows an example of the composition of a cosmetic composition as a formulation having a wrinkle-improving effect containing a royal palm extract of each cosmetic formulation. Here, the extract of Example 2, which has the best effect, was used.

Prescription Example 1: Softening Longevity

Table 5 shows examples of the formulation of the softening water in the cosmetic containing the royal palm extract of Example 2.

ingredient Content (% by weight) Example 2 0.05 Polyoxyethylene hardened castor oil 0.5 Glycine 3.0 Dipotassium glycyrrhizate 0.1 1,3-butylene glycol 3.0 Sodium hyaruronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 antiseptic 0.05 Purified water To 100

Sodium hyaruronate was dispersed in purified water in a propeller mixer at 3000 rpm to prepare a 1% solution. Ingredients 1 to 8 were homogenized in a propeller mixer at 500 rpm and completely dissolved by heating at 75 DEG C, and then cooled to room temperature. Ingredients 9 to 11 were completely dissolved in a separate dissolution tank, and the mixture was added to the above-mentioned water dissolution tank, followed by stirring and mixing. The royal vinegar fruit extract of Example 2 was added thereto and thoroughly stirred and mixed to prepare a softening liquid containing the extract of Example 2.

Prescription Example 2: Nourishing Lotion

Examples of prescription of the nutritional lotion among cosmetics containing at least one royal palm extract of Example 2 are shown in Table 6 below.

ingredient Content (% by weight) Example 2 0.05 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoin 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.3 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 antiseptic 0.05 Purified water To 100

The carbomer was prepared by dispersing the purified water in a propeller mixer at 4000 rpm in a 2% solution. Raw materials 1 to 6 were added to the water-dissolving tank and homogenized at 2000 rpm, dispersed and then heated to 75 캜. Ingredients 7 to 13 were added to an oily dissolution tank and dissolved by heating to 75 ° C. The oil phase dissolved in an aqueous dissolution tank was charged and emulsified (3000 rpm / 5 minutes) and then cooled to room temperature. The royal vinegar fruit extract of Example 2 was added thereto and thoroughly stirred and mixed to prepare a nutrition lotion containing the extract of Example 2.

Prescription Example 3: Essence

Table 7 shows the prescription of essence in cosmetics containing at least one royal palm extract of Example 2.

ingredient Content (% by weight) Example 2 0.05 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyaruronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl cellulose 0.2 ethanol 3.0 Antioxidant 0.3 Triethanolamine 0.1 Polyoxyethylene hardened castor oil 1.0 EDTA 0.1 antiseptic 0.05 Purified water To 100

Sodium hyaruronate and hydroxyethyl cellulose were dispersed in purified water in a propeller mixer at 2000 rpm to prepare a 1% solution. The carbomer was prepared by dispersing the purified water in a propeller mixer at 4000 rpm in a 2% solution. Raw materials 1 to 12 were added to an aqua-melting tank, homogenized at 2000 rpm with stirring, and then heated to 75 캜. The warmed water phase was cooled to room temperature. The raw materials 13 to 15 were completely dissolved in a separate dissolution tank, and the mixture was added to the above-mentioned water dissolution tank and mixed by stirring. The royal vinegar fruit extract of Example 2 was added thereto and thoroughly stirred and mixed to prepare an essence containing the extract of Example 2.

Prescription Example 4: Nourishing Cream

Table 8 shows the prescription examples of the nutritional cream among cosmetics containing at least one royal palm extract of Example 2.

ingredient Content (% by weight) Example 2 0.05 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated lecithin 1.0 Octyldodecanol 3.0 Trioctanoin 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 antiseptic 0.05 Purified water To 100

Raw materials 1 to 8 were added to the water dissolution tank, homogenized at 2000 rpm, dispersed and then heated to 75 캜. The raw material 9-16 was added to the oily melting tank and dissolved by heating to 80 ° C. The oil phase dissolved in an aqueous dissolution tank was charged and emulsified (3000 rpm / 10 minutes) and then cooled to room temperature. The royal vinegar fruit extract of Example 2 was added thereto and thoroughly stirred and mixed to prepare a nutritive cream containing the extract of Example 2.

Experimental Example 5: Effect of improving skin wrinkles

In order to confirm whether the wrinkle-improving effect shown in the above-described experiment actually contributes to the improvement of wrinkles of human skin, a human body application test was conducted on Formulation Example 4 containing Example 2. All tests were conducted with 10 subjects (5 control subjects, 5 test subjects), all female, and the average age was 37 years. 20 ~ 24 ℃, and 45 ~ 55% humidity. After washing, they were measured in a constant temperature and humidity room for at least 1 hour. The skin elasticity was measured 10 times or more before the product was used and 4, 8 and 12 weeks after using the skin elasticity tester (Cutometer MPA 580) for accuracy. The wrinkle improvement measure was also obtained by measuring the shadow area of the wrinkles around the eye area of the subject using a Roboskin analyzer before use and at 4, 8 and 12 weeks after use, Table 9 shows the results.

Example 2 Before use 4 weeks 8 weeks 12 weeks Control Test section Control Test section Control Test section Control Test section outpost Skin elasticity (E / mm) 0.12 0.14 0.11 0.16 0.14 0.25 0.15 0.26 % area 37.3 39.6 35.6 33.7 33.7 32.1 35.8 32.6 Above ground Skin elasticity (E / mm) 0.10 0.19 0.09 0.16 0.15 0.23 0.16 0.22 % area 39.9 42.3 41.6 37.5 43.0 34.6 36.8 37.1 Flower Skin elasticity (E / mm) 0.08 0.12 0.09 0.16 0.16 0.08 0.18 0.22 % area 32.5 29.6 35.1 32.3 30.0 35.6 28.4 34.2 leaf Skin elasticity (E / mm) 0.08 0.11 0.14 0.20 0.16 0.26 0.24 0.29 % area 41.2 45.8 37.7 48.0 38.8 40.1 37.3 32.5 stem Skin elasticity (E / mm) 0.18 0.15 0.13 0.22 0.20 0.27 0.18 0.19 % area 35.6 34.0 36.9 31.2 30.7 33.3 32.5 39.4 Root Skin elasticity (E / mm) 0.09 0.13 0.08 0.09 0.15 0.21 0.15 0.24 % area 44.2 46.8 38.3 42.0 41.2 43.5 41.6 37.7 Fruit Skin elasticity (E / mm) 0.12 0.15 0.15 0.26 0.16 0.32 0.17 0.41 % area 38.7 40.9 40.5 37.7 41.4 31.2 36.9 21.8 strain Skin elasticity (E / mm) 0.12 0.12 0.16 0.20 0.18 0.24 0.20 0.26 % area 28.6 27.4 36.4 34.1 32.6 31.7 33.0 21.3

As a result of measuring the skin wrinkle improving effect on Prescription Example 4 containing the extract of Wang Ho-gang, the best elasticity of Wang Y. fructus showed a tendency that skin elasticity increased drastically at 12 weeks, Also, it was found that it decreased with time, and it was found that it helps to improve the wrinkles of the skin.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments of the present invention are not intended to limit the scope of the present invention but to limit the scope of the present invention. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims (8)

A cosmetic composition for improving skin wrinkles comprising an extract of methanol extracted from at least one region selected from the group consisting of leaves, roots, roots, fruits and seeds except flowers of Reynoutria sachalinensis .
delete delete delete The method according to claim 1,
The extract of Wangwoongjang extract is extracted using at least one extraction method selected from the group consisting of heating at 45 to 120 ° C for 30 minutes to 24 hours followed by warming extraction or immersion at 5 to 40 ° C for 30 minutes to 15 days for shaking Wherein the cosmetic composition is a cosmetic composition for improving skin wrinkles.
The method according to claim 1,
The cosmetic composition for improving skin wrinkles is characterized in that the royal palm extract has 0.01 to 30 wt% based on the total weight of the skin wrinkle improving cosmetic composition.
The method according to claim 1,
The cosmetic composition for improving skin wrinkles is characterized in that the royal palm extract has an ultraviolet ray restoration effect and an MMP-1 collagenase inhibiting effect.
The method according to claim 1,
The cosmetic composition for improving skin wrinkles is characterized in that the cosmetic composition is one of a softening agent, a nutritional lotion, an eye cream, an essence, a nutritional cream, a massage cream, a body cream, a cleansing cream, a cleansing foam, a cleansing water, a powder and a pack.

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