KR102289943B1 - Cosmetic composition containing complex extracts of Chrysanthemum Sibiricum, Viscum Album, Selaginella Tamariscina, Portulaca Oleracea and Perilla Frutescens - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of gujeolcho, mistletoe, rockfish, purslane and lobules. The mixed extract of gujeolcho, mistletoe, rockfish, purslane and lobules of the present invention exhibits excellent antioxidant effect, skin whitening effect, and skin soothing effect, so it can be usefully used as a cosmetic.

Description

Cosmetic composition containing complex extracts of Chrysanthemum Sibiricum, Viscum Album, Selaginella Tamariscina, Portulaca Oleracea and Perilla Frutescens}

The present invention contains the mixed extract of Chrysanthemum Sibiricum, mistletoe (Viscum Album), Selaginella Tamariscina, purslane (Portulaca Oleracea) and leaf leaf (Perilla Frutescens) as an active ingredient, thereby exhibiting excellent antioxidant, whitening and skin soothing activities. It relates to the cosmetic composition shown.

The desire to lead a healthy and youthful life is growing according to the modern people's tendency to place importance on the improvement of living standards and quality of life. Accordingly, in the cosmetic field, the demand for various functional products that go beyond simple makeup and prevent skin aging is increasing.

Melanin is a generic term for brown or black polymer pigments derived from the oxidation of phenols by oxidase. It is the most important factor that determines skin color in the human body, and determines the entire skin color or hair color even with a very small amount, and as a protein-bound polymer, it has the function of absorbing light in a wide range including ultraviolet rays. The production of such melanin is made in an organelle called melanosome (melanosome) in melanocytes in the basal layer. When melanin is produced in an abnormally small amount, skin lesions such as vitiligo are induced. On the contrary, when melanin is excessively synthesized or pigmented by various environmental factors such as sunlight, hormonal changes, inflammation, or drugs, spots and freckles are formed.

In order to prevent pigmentation caused by excessive melanin production and to obtain a whitening effect, it is necessary to reduce melanin production by inhibiting a part of the melanin production process. The most important enzyme in the production of melanin is tyrosinase, which is a tyrosine hydroxylase that oxidizes tyrosine in melanosomes to produce 3,4-dihydroxyphenylalanine (DOPA). It acts as a DOPA oxidase that oxidizes dopachrome to make dopachrome, finally synthesizing melanin polymer.

As active ingredients of whitening functional cosmetics, mulberry extract, arbutin, ethyl ascorbyl ether, oil-soluble licorice extract, ascorbyl glucoside, magnesium ascorbyl phosphate, niacinamide, alpha-bisabolol and ascorbyl tetraisopalmitate are whitening functional ingredients. is announced as

On the other hand, sensitive skin refers to skin that reacts more sensitively than normal skin to external irritants or internal causes of the human body, so that an irritant reaction or dermatitis is easily observed. Recently, due to changes in the living environment, air pollution, stress, etc., more and more people consider themselves sensitive skin. In fact, it is known that skin sensitivity increases due to various causes such as skin diseases, overwork, lack of sleep, stress, food, ultraviolet rays, cosmetics, pollution, and seasonal changes. Sensitive skin can be diagnosed with subjective reactions such as dryness, stinging, or itching after contact with various irritants and objective reactions such as erythema, wheal, papule, blister, pustule, and scale. However, since functional cosmetics that provide various functionalities can act as irritants for sensitive skin, research on ingredients that alleviate skin sensitivity or soothe skin are being conducted in parallel.

The present inventors have tried to develop a cosmetic with skin whitening activity by selecting and combining natural substances with antioxidant activity, and the mixed extracts of gujeolcho, mistletoe, rockfish, purslane and lobules exhibit excellent antioxidant and whitening activity as well as skin soothing. The present invention was completed by confirming that it has an effect.

(0001) Republic of Korea Patent Publication No. 10-2019-0076620 (2019.07.02) (0002) Republic of Korea Patent Registration No. 10-1634668 (2016.06.23)

An object of the present invention is to provide a cosmetic composition containing a mixed extract of gujeolcho, mistletoe, basil, purslane and leaflet, which exhibits excellent antioxidant, whitening and skin soothing effects.

In order to achieve the above object, according to the present invention, there is provided a cosmetic composition containing a mixed extract of gujeolcho, mistletoe, rockfish, purslane and leaflets.

The mixed extract is at least one selected from the group consisting of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethanol, propylene glycol, and butylene glycol of gujeolcho, mistletoe, balsam, purslane and leaflets. It is prepared by extraction with an extraction solvent of

The mixed extract is prepared by mixing Gujeolcho, mistletoe, rock hand, purslane and leaflets in a weight ratio of 1-5:1-5:1-5:1-5:1-5, respectively.

The mixed extract is included in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is characterized in that it is for antioxidant, skin whitening or skin soothing.

According to the present invention, the mixed extract of Gujeolcho, mistletoe, pomegranate purslane, purslane and lobules not only exhibits superior antioxidant and whitening activity by their synergistic action, but also exhibits a skin soothing effect, and thus can be usefully used as a cosmetic.

Hereinafter, the present invention will be described in more detail.

Guilcho (Chrysanthemum zawadskii var. latilobum KITAMURA.) is also called Guilcho (九日草) and Seonmocho (仙母草). The name Gujeolcho is derived from the meaning of grass that is cut nine times, or grass that is cut on the 9th day of the lunar calendar. The height is around 50cm, and it breeds with the underground stem extending sideways. The leaves are ovate or broad ovate, and the edge is divided into an upper right one time. Lateral row pieces (側裂片) are often 4, long oval, with a blunt tip and slightly cracked or serrated edges. The flowers bloom in September-November in pink or white, and hang at the tip of the branch in a capillary inflorescence. The marginal flower of the head inflorescence is a snow flower, and the flowers in the middle are ornamental flowers. The fruit is aqueous (瘦果). It grows in colonies on the ridges of high elevations, but it is also commonly grown in fields. It is distributed nationwide in Korea, and it is also distributed in Japan, Manchuria, and China. Gujeolcho is highly valued as an ornamental due to its beautiful shape, and can also be cultivated. In addition, it has been used as a medicine for gynecological diseases such as menstrual irregularity, uterine coldness, and infertility since ancient times. Sangujeolcho and Bawigujeolcho, similar to Gujeolcho, are also used as folk medicines. In the preparation of the mixed extract of the present invention, the leaves, stems, outages, etc. of Gujeolcho can be used.

Mistletoe (Viscum album) is an evergreen shrub semi-parasitic plant that grows with oak and chestnut trees as hosts. Mistletoe is rooted in the trunk of the host tree and sucks water from the tube and the nutrients made by the host plant in the tube to live and bloom and bear fruit. It also makes its own food through photosynthesis, and its chloroplast is not destroyed even in winter and remains green, so it is also called Dongcheong (凍靑). In folk and oriental medicine, it has been widely used as a medicine for various diseases such as cancer, arteriosclerosis, heart disease, high blood pressure, toothache, and menstrual pain. For the preparation of the mixed extract of the present invention, mistletoe leaves, stems, or grasses may be used.

Selaginella tamariscina (Selaginella tamariscina) is a perennial plant of the lycopod family. The rock hand is also called Buddha hand, Bulsacho, Jangsaengbulsacho, Hoeyangcho, and Gwonbaek. The branches are flattened, the front side is green, and the back side is slightly white. When dry, the branches contract and become like balls, and when wet they open up again. It treats symptoms such as when menstruation is not smooth, when there is a wound due to a bruise, vomiting blood, or blood in the stool. It is also known to have anti-inflammatory and antioxidant properties. For the preparation of the mixed extract of the present invention, it is possible to use the outpost of baekson.

Purslane (Portulaca oleracea L.) is an annual herb and is also called five elements, such as Jangmyungchae, and Machichae. It is a plant that grows obliquely on the ground with many branched branches with brownish-red stems. Dried outplants of purslane (Portulaca oleracea L.) are called machihyun and are used medicinally. In 'Bonchogangmok' and 'Donguibogam', it is recorded that purslane is also used as an antidote for poisonous and dead poison. In the Arabian Peninsula, it is used as an antiseptic, anti-scurvy, antispasmodic, diuretic, anthelmintic, and skin sedative. For the preparation example of the mixed extract of the present invention, it is possible to use purslane outpost.

Leaflet (Perilla frutescens var. acuta) is an annual herb belonging to the family Lamiaceae, native to China, and is a naturalized plant that is imported as a medicinal plant and cultivated in various places in Korea. The stems of leaflets grown by sprinkling seeds in spring have coarse hairs, the leaves have a long stem, and the edges are coarsely serrated. In summer, the stem emerges from the axil at the tip of the stem and small, light purple flowers bloom. The leaves are called leaflets (蘇葉), perilla or chazugi, and the seeds are called soja (蘇子) and are used medicinally. The leaflets have pharmacological effects of excitement, sweating, nausea, sedation, analgesia, diuresis, and stomach, and are also used as a flavoring, seasoning, tonic, food coloring, and cosmetic coloring ingredient. In the preparation of the mixed extract of the present invention, leaves, stems, or foliar leaves, etc. can be used.

Gujeolcho, mistletoe, rock hand, purslane, and leaflets used in the present invention can be purchased offline or in an internet market. In the preparation of the extract of the present invention, gujeolcho, mistletoe, rock hand, purslane, and leaflets are used by washing with purified water and dried and chopped or powdered according to the extraction method.

According to the present invention, there is provided a cosmetic composition containing a mixed extract of gujeolcho, mistletoe, rockfish, purslane and lobules as an active ingredient.

According to one embodiment of the present invention, the mixed extract of Gujeolcho, mistletoe, rock hand, purslane and leaflet is prepared as follows.

After washing, prepare a mixture by mixing dried gujeolcho, mistletoe, rockfish, purslane and leaflets in a weight ratio of 1-5:1-5:1-5:1-5:1-5, respectively. In a conventional method using at least one solvent selected from the group consisting of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethanol, propylene glycol and butylene glycol as an extraction solvent in the mixture extract according to The mixed extract of the present invention may be a concentrated solution under reduced pressure, a powder freeze-dried of the concentrate, or a powder dried by spray-drying the concentrate.

The mixed extract is more excellent in antioxidant, skin whitening and skin soothing when extracted in a weight ratio of 3~5:1:2~3:1:1~2, respectively. Since it exhibits activity, it is more preferable.

The mixed extract exhibited superior antioxidant, skin whitening and skin soothing activity compared to the respective extracts by synergistic action.

The mixed extract is included as an active ingredient in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition. The cosmetic composition may be used for antioxidant, skin whitening or skin soothing.

The cosmetic composition of the present invention is not particularly limited in its formulation, for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream , hand cream, essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder or eye shadow.

In addition, in the cosmetic composition of each formulation, ingredients other than the mixed extract may be arbitrarily selected and formulated according to the formulation or purpose of use of other cosmetics. For example, it may include a colorant (pigment), a flavoring agent (fragrance), a suspending agent, an emulsifier, a solubilizing agent, a stabilizer, an isotonic agent, a pH adjusting agent, a viscosity adjusting agent, a solvent, and the like.

The cosmetic composition of the present invention may include at least one other active ingredient that exhibits skin moisturizing, soothing and regenerating effects in addition to the mixed extract.

Hereinafter, to help the understanding of the present invention, examples will be described in detail. However, the following examples are merely illustrative of the contents of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

[Example]

Preparation Example 1: Preparation of Gujeolcho Extract

After drying, 700 g of purified water and 300 g of ethanol were used as extraction solvents for 100 g of pulverized gujeolcho (starch), followed by hot water extraction at 70-75°C for 2 hours. Thereafter, it was filtered with 300 mesh filter paper, and filtered twice with Adventec No. 5 filter paper and Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure using a vacuum evaporator to obtain an extract.

Preparation Example 2: Preparation of mistletoe extract

After drying, 700 g of purified water and 300 g of ethanol were used as extraction solvents for 100 g of crushed mistletoe (starch), and extraction was performed in a hot water bath at 70-75° C. for 2 hours. Thereafter, it was filtered with 300 mesh filter paper, and filtered twice with Adventec No. 5 filter paper and Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure using a vacuum evaporator to obtain an extract.

Preparation Example 3: Preparation of the extract of basimson

After drying, 700 g of purified water and 300 g of ethanol were used as extraction solvents for 100 g of pulverized basalt grass, and the mixture was extracted in a hot water bath at 70-75°C for 2 hours. Thereafter, it was filtered with 300 mesh filter paper, and filtered twice with Adventec No. 5 filter paper and Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure using a vacuum evaporator to obtain an extract.

Preparation Example 4: Preparation of purslane extract

After drying, 700 g of purified water and 300 g of ethanol were used as extraction solvents for 100 g of pulverized purslane (starch), and extraction was performed in a hot water bath at 70-75°C for 2 hours. Thereafter, it was filtered with 300 mesh filter paper, and filtered twice with Adventec No. 5 filter paper and Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure using a vacuum evaporator to obtain an extract.

Preparation Example 5: Preparation of leaflet extract

After drying, 700 g of purified water and 300 g of ethanol were used as extraction solvents for 100 g of pulverized leaflets (leaf), and extraction was performed in a hot water bath at 70-75°C for 2 hours. Thereafter, it was filtered with 300 mesh filter paper, and filtered twice with Adventec No. 5 filter paper and Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure using a vacuum evaporator to obtain an extract.

Examples 1 to 13: Preparation of mixed extracts of Gujeolcho, mistletoe, rock hand, purslane and lobules

700 g of purified water and 300 g of ethanol were used as extraction solvents for 20 g of dried and pulverized Gujeolcho (outpost), 20 g of mistletoe (outpost), 20 g of rockfish (outpost), 20 g of purslane (outpost), and 20 g of leaflets (leaf), 70-75 The extraction was carried out in a bath at ℃ for 2 hours. Thereafter, it was filtered with 300 mesh filter paper, and filtered twice with Adventec No. 5 filter paper and Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure with a vacuum evaporator to obtain a mixed extract.

100 g of a mixture was prepared by varying the weight ratio as shown in Table 1, and extracted in the same manner to prepare a mixed extract.

weight ratio Gujeolcho mistletoe rock hand purslane leaflet Example 1 One One One One One Example 2 3 One 2 One One Example 3 3 One 2 One 2 Example 4 3 One 3 One One Example 5 3 One 3 One 2 Example 6 4 One 2 One One Example 7 4 One 2 One 2 Example 8 4 One 3 One One Example 9 4 One 3 One 2 Example 10 5 One 2 One One Example 11 5 One 2 One 2 Example 12 5 One 3 One One Example 13 5 One 3 One 2

Test Example 1: Cell viability measurement test

Cell viability measurement experiment (MTT assay)

In order to confirm the cell viability of the extracts of Preparation Examples and Examples, MTT assay was performed as follows.

The MTT assay method is performed after the MTT [3-(4,5-dimethythiasol-2-yl)-2,5-diphenyl tetrazolium bromide] reagent is absorbed into the cells and then formsazan by succinate dehydrogenase in the mitochondria. ), the intracellular accumulation of this substance means mitochondrial activity, broadly, cell activity, and is a representative method for measuring cell viability.

Human-derived fibroblasts were dispensed with 200 μl medium into 96 wells at a density of ^{1×10 5} _{cell/well, incubated for 24 hours in 5% CO 2} , 37℃ incubator, discarded medium, washed with PBS, and the same amount Samples dissolved in medium and PBS were treated in each well according to various concentrations from 1000ppm to 50000ppm and cultured for 24 hours. 4 hours before the end of the incubation, 20 μl of 5 mg/ml MTT dissolved in PBS was added to each well, shielded from light with aluminum foil, and incubated in an incubator under _{5% CO 2 and 37° C. conditions for 3 hours.} The culture medium was removed, 200 μl of DMSO solution was added, and the reaction was performed in an incubator at 37° C. for 1 hour. Then, the absorbance (optical density) was measured at 570 nm using an ELISA reader and compared. Cell viability was calculated by the following formula, and the results are shown in Table 2 below.

Cell viability (%) = O.D. at 570nm / O.D. at 570nm × 100

division Cell viability (%) 1000ppm 5000ppm 10000ppm 50000ppm Example 1 101 100 100 101 Example 2 101 101 100 100 Example 3 101 100 101 100 Example 4 100 100 100 100 Example 5 101 100 100 100 Example 6 100 100 101 101 Example 7 101 100 101 101 Example 8 101 101 100 100 Example 9 101 100 101 100 Example 10 100 100 101 100 Example 11 101 101 100 100 Example 12 100 100 100 101 Example 13 100 100 100 101 Arbutin 100 100 101 101

As confirmed in Table 2, Examples 1 to 13 did not show cytotoxicity.

Test Example 2: Antioxidant activity evaluation

In order to evaluate the antioxidant activity of the extracts of Preparation Examples and Examples, a DPPH free radical scavenging effect assay for DPPH radicals was performed in the following manner.

The DPPH radical scavenging activity was measured by the reducing power of the sample with respect to the DPPH radical. Dissolve DPPH (1,1-Diphenyl-2-picrylhydrazyl) in ethanol to prepare a 0.4 mM solution. Prepare by dissolving BHT (Butylated hydroxyl toluene or 2,6-Di-tert-butyl-p-cresol) used as a standard in ethanol. In a 96-well plate, add 100 μl of prepared samples or BHT and 100 μl of 0.4 mM DPPH solution for each concentration and mix. React at 37°C for 30 minutes. Measure the absorbance at 517 nm using an ELISA reader. The DPPH free radical scavenging ability (%) of the sample or standard (BHT) was calculated using the formula below.

DPPH free radical scavenging ability (%) = {1-(A/B)} × 100

A: STD. or the absorbance of the sample

B: Absorbance of a solution mixed with 0 ppm sample in DPPH solution

The results are shown in Table 3 below.

division DPPH free radical scavenging effect (%) 10 ppm 50 ppm 100 ppm Preparation Example 1 12.05 40.02 62.24 Preparation 2 10.21 38.26 58.24 Preparation 3 11.26 36.18 56.36 Preparation 4 12.04 38.29 60.28 Preparation 5 11.86 34.24 58.38 Example 1 14.02 48.23 68.64 Example 2 16.23 52.04 72.82 Example 3 16.24 54.28 72.76 Example 4 17.06 55.35 74.16 Example 5 16.68 54.62 75.28 Example 6 18.23 56.34 76.34 Example 7 18.26 56.38 77.25 Example 8 19.48 57.27 78.26 Example 9 18.26 56.26 76.26 Example 10 19.24 58.34 78.61 Example 11 22.45 64.27 86.46 Example 12 20.13 58.24 80.48 Example 13 20.24 58.64 78.26 BHT (100ppm) 76.26

As can be seen in Table 3, the mixed extract of the example has improved DPPH free radical scavenging ability in a concentration-dependent manner, and it can be seen that it exhibits better antioxidant activity than the respective extracts of the preparation example.

Test Example 3: Evaluation of whitening activity

In order to evaluate the whitening efficacy of the extracts of Preparation Examples and Examples, a melanin production inhibition test was performed in the following manner.

The melanin-forming cells used in this test example are mouse-derived B16F10 cell lines, which secrete a black pigment called melanin, and were purchased from the Korean Cell Line Bank.

To measure the melanin biosynthesis inhibitory effect of B16F10 melanin-forming cells, the degree of melanin black pigment reduction was evaluated by treating the sample during artificial culture of B16F10 cells. B16F10 melanin-forming cells were aliquoted in a 6-well plate at a concentration of 2×10 ⁶ per well to attach the cells, and then treated with samples for each group at a concentration that does not induce toxicity and cultured for 72 hours.

After incubation, the cells were removed with trypsin-ethylenediamine tetra acetic acid (trypsin-ethylenediamine tetra acetic acid), the number of cells was counted, and the cells were recovered by centrifugation. Quantification of intracellular melanin was performed by modifying the method of Lotan (Lotan: Cancer Res., 40: 3345-3350, 1980), and after washing the cell pellet once with phosphate buffered saline (PBS), a homogenization buffer solution (50 mM 1 ml of sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt cells. After dissolving the extracted melanin by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifuging the disrupted cells (3,000 rpm, 10 minutes), the absorbance of melanin was measured at 405 nm with the microplate reader. Melanin was quantified. The inhibition rate of melanogenesis in B16F10 melanocytes was calculated based on the following formula, and the results are shown in Table 4 below. At this time, arbutin, a substance known to have an inhibitory effect on melanogenesis, was used as a comparison group.

Melanogenesis inhibition rate (%)=[(A-B)/A]×100

A: Amount of melanin in wells to which no sample was added

B: the amount of melanin in the well to which the sample was added

division Melanin production inhibition rate (%) 100 ppm 500 ppm 1000 ppm Preparation Example 1 18.56 21.34 24.54 Preparation 2 12.54 14.26 18.56 Preparation 3 12.05 14.12. 16.34 Preparation 4 14.25 18.46 20.32 Preparation 5 14.23 18.65 22.84 Example 1 20.05 23.46 26.32 Example 2 22.86 24.36 28.52 Example 3 23.05 25.12 28.83 Example 4 24.02 26.14 29.46 Example 5 24.65 27.12 30.08 Example 6 24.26 28.76 30.12 Example 7 24.46 28.72 32.24 Example 8 25.02 28.86 34.21 Example 9 24.84 26.24 33.12 Example 10 25.04 30.12 32.26 Example 11 26.25 34.26 40.05 Example 12 25.46 30.42 32.28 Example 13 26.06 32.04 38.46 arbutin
(1000ppm) 40.26

As can be seen in Table 4, the mixed extract of Examples of the present invention exhibited an excellent inhibition rate of melanin production compared to each extract of Preparation Examples. Examples 2 to 13 showed a more excellent effect than Example 1, and Example 11 showed the most excellent effect.

Formulation Example 1: Preparation of skin lotion

According to the composition of Table 5 below, 1 kg of skin lotion containing the mixed extract was prepared by the following method.

number Raw material content (wt%) One Mixed extract (Example 11) 1.0 2 glycerin 3.0 3 butylene glycol 2.0 4 propylene glycol 2.0 5 Polyoxyethylene hydrogenated castor oil 1.0 6 triethanolamine 0.1 7 antiseptic a very small amount 8 pigment a very small amount 9 Spices a very small amount 10 Purified water remaining amount

In Table 5, materials 2, 3, 4, and 7 were sequentially put into a reaction vessel and dissolved by stirring, and then, material 5 was heated to about 60° C. to dissolve. Thereafter, after dissolving the 9th material was added to the 10th material. Finally, substances 1, 6, and 8 were added, sufficiently stirred, and aged at 25° C. for 3 days to prepare the title skin lotion.

Formulation Example 2: Preparation of Nutrient Lotion

According to the composition of Table 6 below, 1 kg of hydrosome nutritional lotion containing the mixed extract was prepared by the following method.

number Raw material content (wt%) One Mixed extract (Example 11) 1.0 2 beeswax 1.0 3 Polysorbate 60 1.5 4 Sorbitan Sesquiolate 0.5 5 liquid paraffin 10.0 6 Sorbitan Stearate 1.0 7 lipophilic monostearate glycerin 0.5 8 stearic acid 1.5 9 Glyceryl Stearate/PEG-400 Stearate 1.0 10 propylene glycol 3.0 11 carboxy polymer 0.1 12 triethanolamine 0.2 13 antiseptic a very small amount 14 pigment a very small amount 15 Spices a very small amount 16 Purified water remaining amount

In Table 6, materials No. 10, 11, 13, and 16 were mixed and stirred, heated to between 80 and 85° C., put into the manufacturing unit, and then the emulsifier was acted on, and 2, 3, 4, 5, 6, 7, 8, 9 , material 12 was dissolved by heating at 80~85℃ and then emulsified. After the emulsification is finished, use a stirrer to cool to 50°C, add material 15, cool to 45°C, add material 14, add material 1 at 35°C, cool to 25°C, and then cool to 25°C The title nutritional lotion was prepared by aging for 3 days.

Test Example 4: Confirmation of skin soothing effect

After inducing skin irritation through the Tape Stripping Method on the inner part of the lower arm for 12 men and women in their 20s and 30s who do not currently have skin diseases or skin allergy symptoms, the extract containing the mixed extract according to the present invention The degree of calming of irritation induced by applying the skin lotion of Formulation Example 1 was evaluated relative to each other. A skin lotion containing no extract of the present invention was used as a control example.

First, skin erythema was induced by taping 20 to 30 times (the frequency is adjusted according to the level of skin sensitivity of the volunteer) with general scotch tape in two places on the left and right sides of the inner part of the lower arm. After erythema was induced, the cosmetic composition of Formulation Example 1 according to the present invention was applied on the left side, and the control example cosmetic composition without the mixed extract according to the present invention was applied on the right side. The degree of skin soothing was evaluated 2 hours after application.

<Evaluation of skin irritation level (erythema)>

0 (no response) 1 (very slight erythema) 2 (faint or mild erythema)

3 (demarcated but mild erythema) 4 (clear erythema) 5 (severe erythema)

test subject Early Formulation Example 1
(left) contrast example
(right) left right One 4 4 One 3 2 3 3 One 3 3 2 2 0 One 4 3 3 0 2 5 3 3 0 2 6 2 2 One One 7 3 3 0 2 8 3 3 0 One 9 4 4 One 3 10 2 2 One One 11 3 3 0 One 12 4 4 One 2

As can be seen in Table 7 above, it can be seen that the cosmetic composition containing the mixed extract of gujeolcho, mistletoe, pomegranate purslane, purslane and lobules of the present invention as an active ingredient has a high skin soothing effect.

Although the present invention has been described in detail so far, it is clear that the embodiments mentioned in the process are only illustrative and not restrictive, and the present invention is the technical spirit or field of the present invention provided by the following claims Within a range that does not depart from the above, it will be said that component changes to a degree that can be uniformly dealt with fall within the scope of the present invention.

Claims (7)

Gujeolcho, mistletoe, rock hand, purslane and leaflet are mixed in a weight ratio of 5:1:2:1:2, respectively, and the mixed extract prepared by extracting gujeolcho, mistletoe, rock hand, purslane and leaflet is 0.01 based on the total weight of the cosmetic composition. A cosmetic composition for skin soothing containing ~30% by weight. The method of claim 1, wherein the mixed extract is made of Gujeolcho, mistletoe, purslane and leaflets of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethanol, propylene glycol and butylene glycol. A cosmetic composition for skin soothing, characterized in that it is prepared by extraction with at least one extraction solvent selected from the group. delete delete delete delete delete