KR20210031264A - Cosmetic composition containing complex extracts of Chrysanthemum Sibiricum, Viscum Album, Selaginella Tamariscina, Portulaca Oleracea and Perilla Frutescens - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of Chrysanthemum sibiricum, Viscum album, Selaginella tamariscina, Portulaca oleracea and Perilla frutescens. The mixed extract of Chrysanthemum sibiricum, Viscum album, Selaginella tamariscina, Portulaca oleracea and Perilla frutescens of the present invention exhibits excellent antioxidant effects, skin whitening effects, and skin soothing effects, and thus can be usefully used as cosmetic materials.

Description

Cosmetic composition containing complex extracts of Chrysanthemum Sibiricum, Viscum Album, Selaginella Tamariscina, Portulaca Oleracea and Perilla Frutescens}

The present invention contains a mixed extract of Gujeolcho (Chrysanthemum Sibiricum), mistletoe (Viscum Album), Selaginella Tamariscina, Purslane (Portulaca Oleracea) and lobule (Perilla Frutescens) as active ingredients, and has excellent antioxidant, whitening and skin soothing activities. It relates to the cosmetic composition shown.

Modern people's desire to lead a healthy and young life is growing in line with the increasing standard of living and the tendency to value quality of life. Accordingly, in the cosmetic field, there is an increasing demand for various functional products that prevent skin aging beyond simple makeup.

Melanin (melanin) is a generic term for brown or black polymer pigments induced by oxidation of waste nools by oxidative enzymes. It is the most important factor in determining skin color in the human body, and as a polymer in a form combined with protein, it has the function of absorbing light in a wide area including ultraviolet rays. The production of melanin occurs in an organelle called melanosomes in melanocytes in the basal layer. When melanin is produced in an abnormally small amount, skin lesions such as vitiligo are caused. Conversely, when melanin is excessively synthesized or pigmented due to various environmental factors such as sunlight, hormonal changes, inflammation or drugs, spots and freckles are formed.

In order to prevent pigmentation due to excessive melanin production and obtain a whitening effect, it is necessary to reduce the production of melanin by inhibiting a part of the melanin production process. The enzyme that plays the most important role in the production of melanin is tyrosinase, and DOPA is a tyrosine hydroxylase that oxidizes tyrosine in melanosomes to make DOPA (3,4-dihydroxyphenylalanine). It acts as DOPA oxidase, which oxidizes dopachrome, thereby finally synthesizing melanin polymer.

As active ingredients of whitening functional cosmetics, whitening functional ingredients such as mulberry extract, arbutin, ethyl ascorbyl ether, oil-soluble licorice extract, ascorbyl glucoside, magnesium ascorbyl phosphate, niacinamide, alpha-bisabolol and ascorbyl tetraisopalmitate It is announced as.

On the other hand, sensitive skin refers to skin that reacts more sensitively to external irritants or internal causes of the human body than that of normal skin, so that irritation reactions or dermatitis are more likely. Recently, people who think of themselves as sensitive skin due to changes in living environment, air pollution, and stress are increasing. In fact, it is known that skin sensitivity increases due to various causes such as skin diseases, overwork, lack of sleep, stress, food, ultraviolet rays, cosmetics, pollution, and seasonal changes. Sensitive skin can be diagnosed by subjective reactions that complain of dry, itchy, or itchy after contact with various irritants, and objective reactions such as erythema, swelling, papules, blisters, pustules, and scales. However, since functional cosmetics that impart various functions can function as irritants for sensitive skin, studies on skin sensitivity relief or skin soothing ingredients are being conducted at the same time.

The present inventors have tried to develop a cosmetic material having skin whitening activity by selecting and combining natural products with antioxidant activity, and the mixed extract of Gujeolcho, mistletoe, rock hand, purslane and lobules exhibits excellent antioxidant and whitening activity, as well as skin soothing. The present invention was completed by confirming that it has an effect.

(0001) Korean Patent Application Publication No. 10-2019-0076620 (2019.07.02) (0002) Korean Patent Registration No. 10-1634668 (2016.06.23)

An object of the present invention is to provide a cosmetic composition containing Gujeolcho, mistletoe, rock hand, purslane and lobular mixed extracts, and exhibiting excellent antioxidant, whitening, and skin soothing effects.

In order to achieve the above object, according to the present invention, there is provided a cosmetic composition containing a mixed extract of Gujeolcho, mistletoe, rock hand, purslane and lobules.

The mixed extract is at least one selected from the group consisting of Gujeolcho, mistletoe, rock hands, purslane and lobules in water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethanol, propylene glycol, and butylene glycol It is produced by extraction with an extraction solvent of.

The mixed extract is prepared by mixing Gujeolcho, mistletoe, rock hand, purslane and lobules in a weight ratio of 1-5:1-5:1-5:1-5:1-5, respectively.

The mixed extract is contained in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is characterized in that for antioxidant, skin whitening or skin soothing.

The mixed extract of Gujeolcho, mistletoe, rock hand, purslane and lobules according to the present invention not only exhibits more excellent antioxidant and whitening activity by its synergistic action, but also exhibits skin soothing effects, so it can be usefully used as a cosmetic.

Hereinafter, the present invention will be described in more detail.

Gujeolcho (Chrysanthemum zawadskii var.latilobum KITAMURA.) is also known as Guilcho (九日草) and Sunmocho (仙母草). The name Gujeolcho comes from the meaning of grass that breaks nine times, or grass that breaks on the 9th day of the lunar calendar. It is about 50cm in height and breeds while the inner stem of the ground extends sideways. The leaves are ovate or broad ovate, and the edge is divided into one-time right shape. Pyeonyeolpyeon (側裂片) is often 4 long oval, blunt end, slightly cracked or saw teeth. Flowers bloom in pink or white in September-November, and hangs as a head flower at the end of a branch. The marginal flowers of the head flower book are snowy flowers, and the flowers in the middle are ornamentals. The fruit is a fruit. It grows by forming a colony on the ridges of the highlands, but it is also commonly grown in the fields. It is distributed nationwide in our country, and is also distributed in Japan, Manchuria, and China. Gujeolcho has a beautiful shape and is highly valued for ornamental purposes, and it can be cultivated. Also, since ancient times, it has been used as a medicine for women's diseases such as menstrual impurity, poor uterus, and infertility. Sangujeolcho and rocky gujeolcho, similar to Gujeolcho, are also used as civilian medicines. In the preparation of the mixed extract of the present invention, leaves, stems, outposts, etc. of Gujeolcho can be used.

Mistletoe (Mistletoe, Viscum album) is an evergreen shrub semi-parasitic plant that grows with oak and chestnut as hosts. It is divided into several families and species depending on the host plant, and it is known that there are about 1,500 species of 30 genera worldwide. Mistletoe leaves its roots in the trunk of a host tree, and in the phloem, it sucks water from the nutrients made by the host plant and lives, blooms flowers, and even bears fruit. It photosynthesizes to make its own nutrients, and its chloroplasts are not destroyed even in winter, so they remain green and are called dongcheong (凍靑). It has been widely used as a medicine for various diseases such as cancer, arteriosclerosis, heart disease, high blood pressure, toothache, and menstrual pain in private and oriental medicine. In the preparation of the mixed extract of the present invention, leaves, stems, and outposts of mistletoe may be used.

The rock hand (Selaginella tamariscina) is a perennial plant in the family of Buddha-son of the Seoksong tree. Rock hands are also called Buddha hands, Buddha candles, Jangsaeng Buddha candles, Hoe candles, and Gwonbaek. Branches are split flat, and the front side is green and the back side is slightly white. When it is dry, the branches contract and become like a ball, and when there is moisture, they spread out again. When menstruation is not going smoothly, when a wound has occurred such as a bruise, vomiting blood or showing blood in the stool, etc. are treated. It is also known to have anti-inflammatory and antioxidant properties. In the preparation of the mixed extract of the present invention, it is possible to use an outpost of rock son.

Purslane (Portulaca oleracea L.) is a one-year herb, and is sometimes called as five, jangmyeongchae, and machichae. It mainly grows wildly from May to September in roadsides and gardens, and the height of the stem is about 15~30cm. No, the stem is brownish red, and the branches are split a lot, and it is a plant that grows while spreading diagonally above the ground. The dried outpost of Purslane (Portulaca oleracea L.) is called machihyeon and is used as a medicinal material. In'Bonchogangmok' and'Donguibogam', it is recorded that purslane is also used as an antidote for poisoning and sadok. In the Arabian Peninsula, it is used as an antiseptic, antiscurvy, antispasmodic, diuretic, anthelmintic, and skin soothing agent. Examples of the preparation of the mixed extract of the present invention may use the outpost of purslane.

Perilla frutescens var. acuta is an annual herb belonging to the Lamiaceae family. It is a naturalized plant native to China and imported as a medicinal plant and cultivated in various places in Korea. The stems of lobules grown by sowing seeds in spring have loose hairs, the leaves have long stems, and the edges are sparse serrated. In summer, the stem comes out of the leaf axilla at the end of the stem, and small light purple flowers bloom. Leaves are called lobules, perilla, or chazugi, and seeds are called soja (蘇子) and are used as medicinal materials. The lobules have the pharmacological action of excitement, sweating, jihae (止咳), soothing, pain relief, diuresis, and dry stomach, and are also used as ingredients for flavoring, seasoning, tonic, food coloring and cosmetic coloring. In the preparation of the mixed extract of the present invention, leaves, stems, and outposts of lobules can be used.

Gujeolcho, mistletoe, rock hands, purslane, and lobules used in the present invention can be purchased offline or in the Internet market. In the preparation of the extract of the present invention, Gujeolcho, mistletoe, rock hand, purslane, and lobules are cleaned with purified water and used, and dried and minced according to the extraction method, or may be used as powder.

According to the present invention, there is provided a cosmetic composition containing a mixed extract of Gujeolcho, mistletoe, rock hand, purslane and lobules as an active ingredient.

According to an embodiment of the present invention, the mixed extract of Gujeolcho, mistletoe, rock hand, purslane and lobules is prepared as follows.

After washing, dried Gujeolcho, mistletoe, rock hand, purslane, and lobules are mixed in a weight ratio of 1-5:1-5:1-5:1-5:1-5, respectively, to prepare a mixture. In the mixture, as an extraction solvent, at least one solvent selected from the group consisting of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethanol, propylene glycol and butylene glycol is used in a conventional method. Extract accordingly. The mixed extract of the present invention may be a vacuum concentrate, a freeze-dried powder of the concentrate, or a spray-dried powder of the concentrate.

The mixed extract is more excellent antioxidant, skin whitening and skin soothing when Gujeolcho, mistletoe, rock hand, purslane and lobules are mixed in a weight ratio of 3-5:1:2-3:1:1-2, respectively. It is more preferable because it exhibits activity.

The mixed extract exhibited more excellent antioxidant, skin whitening and skin soothing activities than each extract by synergistic action.

The mixed extract is included as an active ingredient in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition. The cosmetic composition may be used for antioxidant, skin whitening or skin soothing.

The cosmetic composition of the present invention is not particularly limited in its formulation, and for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream , Hand cream, essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, or eye shadow formulation.

In addition, in the cosmetic composition of each formulation, other ingredients other than the mixed extract may be arbitrarily selected and blended according to the formulation or purpose of use of other cosmetics. For example, a colorant (pigment), a flavoring agent (perfume), a suspending agent, an emulsifying agent, a solubility aid, a stabilizer, an isotonic agent, a pH adjusting agent, a viscosity adjusting agent, a solvent, and the like may be included.

The cosmetic composition of the present invention may contain one or more other active ingredients exhibiting skin moisturizing, soothing and regenerating effects in addition to the mixed extract.

Hereinafter, examples will be described in detail to aid understanding of the present invention. However, the following examples are merely illustrative of the contents of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely describe the present invention to those of ordinary skill in the art.

[Example]

Preparation Example 1: Preparation of Gujeolcho extract

After drying, 700g of purified water and 300g of ethanol were used as an extraction solvent in 100g of pulverized Gujeolcho (outpost), followed by extraction in a hot water bath at 70-75°C for 2 hours. After that, it was filtered through a 300 mesh filter paper, and then filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure with a vacuum evaporator to obtain an extract.

Preparation Example 2: Preparation of mistletoe extract

After drying, 700 g of purified water and 300 g of ethanol were used as an extraction solvent to 100 g of pulverized mistletoe (outpost), followed by extraction with a bath for 2 hours at 70-75°C. After that, it was filtered through a 300 mesh filter paper, and then filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure with a vacuum evaporator to obtain an extract.

Preparation Example 3: Preparation of rock hand extract

After drying, 700g of purified water and 300g of ethanol were used as an extraction solvent in 100g of pulverized rock hand (outpost), followed by extraction in a hot water bath at 70-75°C for 2 hours. After that, it was filtered through a 300 mesh filter paper, and then filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure with a vacuum evaporator to obtain an extract.

Preparation Example 4: Preparation of purslane extract

After drying, 700g of purified water and 300g of ethanol were used as an extraction solvent to 100g of pulverized purslane (outpost), and the mixture was extracted with a bath for 2 hours at 70-75°C. After that, it was filtered through a 300 mesh filter paper, and then filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure with a vacuum evaporator to obtain an extract.

Preparation Example 5: Preparation of leaflet extract

After drying, 700 g of purified water and 300 g of ethanol were used as an extraction solvent for 100 g of pulverized leaflets (leaves), and extraction was performed in a water bath at 70-75°C for 2 hours. After that, it was filtered through a 300 mesh filter paper, and then filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure with a vacuum evaporator to obtain an extract.

Examples 1 to 13: Preparation of Gujeolcho, mistletoe, rock hand, purslane and lobular mixed extract

After drying, pulverized Gujeolcho (outpost) 20g, mistletoe (outpost) 20g, rock hand (outpost) 20g, purslane (outpost) 20g, lobules (leaves) 20g, purified water 700g and ethanol 300g were used as extraction solvents, 70-75 The bath was extracted for 2 hours at °C. After that, it was filtered through a 300 mesh filter paper, and then filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. The filtrate was concentrated under reduced pressure with a vacuum evaporator to obtain a mixed extract.

A mixture of 100 g was prepared by varying the weight ratio as shown in Table 1 below, and extracted in the same manner to prepare a mixed extract.

Weight ratio Gujeolcho mistletoe Rock hand purslane Leaflet Example 1 One One One One One Example 2 3 One 2 One One Example 3 3 One 2 One 2 Example 4 3 One 3 One One Example 5 3 One 3 One 2 Example 6 4 One 2 One One Example 7 4 One 2 One 2 Example 8 4 One 3 One One Example 9 4 One 3 One 2 Example 10 5 One 2 One One Example 11 5 One 2 One 2 Example 12 5 One 3 One One Example 13 5 One 3 One 2

Test Example 1: Cell viability measurement test

Cell viability measurement experiment (MTT assay)

In order to confirm the cell viability of the extracts of Preparation Examples and Examples, MTT assay was performed in the following manner.

MTT assay was carried out by MTT [3-(4,5-dimethythiasol-2-yl)-2,5-diphenyl tetrazolium bromide] reagent absorbed into the cell, followed by succinate dehydrogenase of mitochondria, formazan (formazan) ), and the accumulation of this substance in the cell means the activity of mitochondria, broadly the activity of cells, and is a representative method of measuring the survival rate of cells.

Human-derived fibroblasts were dispensed with 200 µl medium in 96 wells at a density of ^{1×10 5} _{cells/well, incubated in 5% CO 2} , 37°C incubator for 24 hours, discarded the medium, washed with PBS, and then the same amount The samples dissolved in the medium and PBS were treated in each well according to various concentrations from 1000ppm to 50000ppm and cultured for 24 hours. 4 hours before the cultivation was completed, 20 µl of 5 mg/ml MTT dissolved in PBS was added to each well, shaded with aluminum foil, and incubated in an incubator under conditions of _{5% CO 2 and 37°C for 3 hours.} After removing the culture medium, adding 200 µl of DMSO solution and reacting for 1 hour in an incubator at 37°C, the optical density was measured and compared at 570 nm using an ELISA reader. Cell viability was calculated by the following formula, and the results are shown in Table 2 below.

Cell viability (%) = O.D. of the sample addition group. at 570 nm/O.D. of no sample added group. at 570nm × 100

division Cell viability (%) 1000ppm 5000ppm 10000ppm 50000ppm Example 1 101 100 100 101 Example 2 101 101 100 100 Example 3 101 100 101 100 Example 4 100 100 100 100 Example 5 101 100 100 100 Example 6 100 100 101 101 Example 7 101 100 101 101 Example 8 101 101 100 100 Example 9 101 100 101 100 Example 10 100 100 101 100 Example 11 101 101 100 100 Example 12 100 100 100 101 Example 13 100 100 100 101 Arbutin 100 100 101 101

As can be seen in Table 2 above, none of Examples 1 to 13 showed cytotoxicity.

Test Example 2: Evaluation of antioxidant activity

In order to evaluate the antioxidant activity of the extracts of Preparation Examples and Examples, a DPPH free radical scavenging effect assay against DPPH radical was performed by the following method.

DPPH radical scavenging activity was measured by the reducing power of the sample against DPPH radicals. To prepare a 0.4mM solution by dissolving DPPH (1,1-Diphenyl-2-picrylhydrazyl) in ethanol. Prepared by dissolving BHT (Butylated hydroxyl toluene or 2,6-Di-tert-butyl-p-cresol) used as a standard in ethanol. In a 96 well plate, 100 µl of prepared sample or BHT and 100 µl of 0.4 mM DPPH solution are added and mixed. It was reacted at 37°C for 30 minutes. Absorbance is measured at 517 nm using an ELISA reader. DPPH free radical scavenging activity (%) of the sample or standard (BHT) was calculated using the following calculation formula.

DPPH free radical scavenging activity (%) = {1-(A/B)} × 100

A: STD. Or the absorbance of the sample

B: Absorbance of a solution obtained by mixing 0 ppm sample with DPPH solution

The results are shown in Table 3 below.

division DPPH free radical scavenging effect(%) 10 ppm 50 ppm 100 ppm Manufacturing Example 1 12.05 40.02 62.24 Manufacturing Example 2 10.21 38.26 58.24 Manufacturing Example 3 11.26 36.18 56.36 Manufacturing Example 4 12.04 38.29 60.28 Manufacturing Example 5 11.86 34.24 58.38 Example 1 14.02 48.23 68.64 Example 2 16.23 52.04 72.82 Example 3 16.24 54.28 72.76 Example 4 17.06 55.35 74.16 Example 5 16.68 54.62 75.28 Example 6 18.23 56.34 76.34 Example 7 18.26 56.38 77.25 Example 8 19.48 57.27 78.26 Example 9 18.26 56.26 76.26 Example 10 19.24 58.34 78.61 Example 11 22.45 64.27 86.46 Example 12 20.13 58.24 80.48 Example 13 20.24 58.64 78.26 BHT(100ppm) 76.26

As can be seen in Table 3, it can be seen that the mixed extract of the Example has improved DPPH-free radical scavenging ability depending on the concentration, and exhibits more excellent antioxidant activity than each of the extracts of the Preparation Example.

Test Example 3: Evaluation of whitening activity

In order to evaluate the whitening efficacy of the extracts of Preparation Examples and Examples, a melanin production inhibition test was performed in the following manner.

The melanin-forming cells used in this test example were mouse-derived B16F10 cell lines, cells that secrete a black pigment called melanin, and were sold and used from the Korean Cell Line Bank.

To measure the inhibitory effect of melanin biosynthesis of B16F10 melanogenic cells, the degree of melanin black pigment reduction was compared and evaluated by treating the sample during artificial culture of B16F10 cells. B16F10 melanin-forming cells were dispensed into a 6-well plate at a concentration of 2×10 ⁶ per well, attached to the cells, and then treated with samples for each group at a concentration that did not cause toxicity, and cultured for 72 hours.

After cultivation, the cells were separated with trypsin-ethylenediamine tetra acetic acid (EDTA), the number of cells was measured, and then the cells were recovered by centrifugation. The quantification of intracellular melanin was performed by modifying the method of Rotan (Cancer Res., 40: 3345-3350, 1980), and the cell pellet was washed once with PBS (phosphate buffered saline), and then homogenized buffer (50 mM). Sodium phosphate, pH 6.8, 1% Triton X-100, 2mM PMSF) 1 ml was added and vortexed for 5 minutes to disrupt the cells. After dissolving the extracted melanin by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifuging the crushed cells (3,000 rpm, 10 minutes), the absorbance of melanin was measured at 405 nm with the microplate reader. Melanin was quantified. The inhibition rate of melanin production of B16F10 melanogenic cells was calculated based on the following formula, and the results are shown in Table 4 below. At this time, arbutin, a substance known to have an inhibitory effect on melanogenesis, was used as a control group.

Melanogenesis inhibition rate (%) = [(A-B)/A]×100

A: the amount of melanin in the well without adding the sample

B: The amount of melanin in the well to which the sample was added

division Melanogenesis inhibition rate (%) 100 ppm 500 ppm 1000 ppm Manufacturing Example 1 18.56 21.34 24.54 Manufacturing Example 2 12.54 14.26 18.56 Manufacturing Example 3 12.05 14.12. 16.34 Manufacturing Example 4 14.25 18.46 20.32 Manufacturing Example 5 14.23 18.65 22.84 Example 1 20.05 23.46 26.32 Example 2 22.86 24.36 28.52 Example 3 23.05 25.12 28.83 Example 4 24.02 26.14 29.46 Example 5 24.65 27.12 30.08 Example 6 24.26 28.76 30.12 Example 7 24.46 28.72 32.24 Example 8 25.02 28.86 34.21 Example 9 24.84 26.24 33.12 Example 10 25.04 30.12 32.26 Example 11 26.25 34.26 40.05 Example 12 25.46 30.42 32.28 Example 13 26.06 32.04 38.46 Arbutin
(1000ppm) 40.26

As can be seen in Table 4, the mixed extract of Examples of the present invention exhibited superior melanin production inhibition compared to each of the extracts of Preparation Example. Examples 2 to 13 exhibited a better effect than Example 1, and Example 11 exhibited the most excellent effect.

Formulation Example 1: Preparation of skin lotion

According to the composition of Table 5 below, 1 kg of skin lotion containing the mixed extract was prepared by the following method.

number Raw material Content (% by weight) One Mixed extract (Example 11) 1.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene hydrogenated castor oil 1.0 6 Triethanolamine 0.1 7 antiseptic a very small amount 8 Pigment a very small amount 9 Spices a very small amount 10 Purified water Balance

In Table 5, materials 2, 3, 4, and 7 were sequentially added to the reaction vessel and dissolved in the reaction vessel, followed by heating and dissolving the 5 material at about 60°C. Thereafter, material No. 9 was added to dissolve and then added to material No. 10. Finally, substances 1, 6, and 8 were added, sufficiently stirred, and aged at 25° C. for 3 days to prepare the title skin lotion.

Formulation Example 2: Preparation of nutritional lotion

According to the composition of Table 6 below, 1 kg of a hydrosome nutritional lotion containing the mixed extract was prepared by the following method.

number Raw material Content (% by weight) One Mixed extract (Example 11) 1.0 2 Beeswax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbitan stearate 1.0 7 Glycerin monostearate 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate/Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxypolymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Balance

In Table 6, materials 10, 11, 13, and 16 were mixed and stirred, heated between 80 and 85°C, added to the manufacturing unit, and then reacted with an emulsifier and 2, 3, 4, 5, 6, 7, 8, 9 , Material No. 12 was dissolved by heating between 80 and 85°C, and then emulsified. After the emulsification is finished, cool it to 50℃ while stirring with a stirrer, then add the 15th material, cool to 45℃, add the 14th material, add the 1st material at 35℃, cool it to 25℃, and then 25℃ It was aged for 3 days to prepare the title nutritional lotion.

Test Example 4: Confirmation of skin soothing effect

After inducing skin irritation through the tape stripping method on the inner part of the lower arm targeting 12 men and women in their 20s to 30s who do not have skin diseases or skin allergy symptoms, the above containing the mixed extract according to the present invention The degree to which the irritation caused by applying the skin lotion of Formulation Example 1 was calmed was evaluated relative. A skin lotion not containing the mixed extract of the present invention was used as a control example.

First, the two places on the left and right of the inner part of the lower arm were taped 20 to 30 times with a regular scotch tape (the number of times was adjusted according to the degree of skin sensitivity of the volunteer) to induce skin erythema. After erythema was induced, the cosmetic composition of Formulation Example 1 according to the present invention was applied on the left side, and the control example cosmetic composition not containing the mixed extract according to the present invention was applied on the right side. The degree of skin soothing was evaluated 2 hours after application.

<Evaluation of skin irritation degree (erythema)>

0 (no reaction) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear border but weak erythema) 4 (clear erythema) 5 (severe erythema)

Test subject Early Formulation Example 1
(Left) Contrast
(Right) Left Ooh One 4 4 One 3 2 3 3 One 3 3 2 2 0 One 4 3 3 0 2 5 3 3 0 2 6 2 2 One One 7 3 3 0 2 8 3 3 0 One 9 4 4 One 3 10 2 2 One One 11 3 3 0 One 12 4 4 One 2

As shown in Table 7, it can be seen that the cosmetic composition containing the extract of Gujeolcho, mistletoe, rock hand, purslane and lobules as an active ingredient of the present invention has a high skin soothing effect.

Although the present invention has been described in detail so far, it is clear that the embodiments mentioned in the process are only illustrative and not limiting, and the present invention is provided by the following claims. Within the range not departing from, it will be said that component changes to the extent that can be coped with equally fall within the scope of the invention.

Claims (7)

A cosmetic composition containing Gujeolcho, mistletoe, rock hand, purslane and lobular mixture extract. The method of claim 1, wherein the mixed extract is made of Gujeolcho, mistletoe, rock hand, purslane and lobules in water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethanol, propylene glycol, and butylene glycol. Cosmetic composition, characterized in that produced by extraction with at least one extraction solvent selected from the group. The method of claim 1, wherein the mixed extract is prepared by mixing Gujeolcho, mistletoe, rock hand, purslane and lobules in a weight ratio of 1-5:1-5:1-5:1-5:1-5, respectively. Cosmetic composition, characterized in that. The cosmetic composition according to claim 1, wherein the mixed extract is contained in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is for antioxidant. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin whitening. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin soothing.