KR102054793B1 - Cosmetic composition Cosmetic for improving skin elasticity and reducing dark circles containing natural extract and fermented extract - Google Patents

Abstract

The present invention, as a nature-friendly cosmetic composition prepared only with natural ingredients, excluding chemical components as much as possible, relates to a skin antioxidant cosmetic composition containing a fermented extract and a natural extract, being skin-friendly and having skin moisturizing, antioxidant activity, and an excellent whitening function; and a manufacturing method thereof. More specifically, the composition comprises purified water, a mixture A for moisturizing and nourishing function, a mixture B for reducing wrinkles and antioxidant function, a mixture C for skin moisturizing and formulation stabilizing function, a mixture D comprising a natural extract and a fermented extract, and fragrant materials.

Description

Antioxidant cosmetic composition for improving skin elasticity and dark circle relief containing natural extracts and fermented extracts and a method of manufacturing the same {Cosmetic composition Cosmetic for improving skin elasticity and reducing dark circles containing natural extract and fermented extract}

The present invention is a natural-friendly cosmetic composition prepared only with natural ingredients that exclude chemical ingredients as much as possible, skin moisturizing as well as antioxidant activity, excellent whitening function and skin-friendly natural extracts and fermented extracts, skin elasticity improvement and dark circle relief It relates to a cosmetic composition for and a method for producing the same.

As human skin ages, the decrease in hormone secretion and immune cell function decreases the synthesis of skin proteins, which leads to natural skin aging. In addition, the increase of ultraviolet rays and various oxidizing substances cause damage and abnormal proliferation of keratinocytes. Damage or deterioration of the skin cells causes fatal phenomena on skin beauty such as decreased skin elasticity, increased wrinkles, and increased blemishes. External stimulation releases various cytokines, cytokines (cytokine) from skin keratinocytes, immune cells, and melanocyte synthesis cells.TGFbeta (transforming growth factor), TNFalpha (tumor necrosis factor), PGE2 (prostaglandin E2), and alpha -MSH (melanocyte stimulating hormone), GSF (granulocyte stimulating factor), IL-1 (interleukin-1), IL-6, IL-8, IL-10 is a substance secreted from keratinocytes. α-MSH is a type of growth hormone that is released from skin keratinocytes when exposed to external stress such as UV and binds to receptors present in melanocytes. TGFbeta, PGE2, and α-MSH are secreted from keratinocytes. Induces the activation of melanin-forming cells that synthesize melanin to increase melanin synthesis, secreted melanin is delivered to keratinocytes to suppress the cellular damage caused by stress. However, brown pigments and black pigments produced in melanocytes have negative effects on skin cosmetics, and there have been various attempts in the cosmetic industry to block melanin synthesis (Tobin DJ, Shibahara S, Slominski A, Melanin pigmentation in mammalian). skin and its hormonal regulation.J. Physiol Rev, 84 (4), pp 1115-228, 2004).

Melanin synthesis occurs through the action of tyrosinase present in melanosomes. This tyrosinase acts as a tyrosine hydroxylase that oxidizes L-tyrosine to form L-DOPA, and is also known as an important enzyme for synthesizing melanin by acting as a DOPA oxidant which oxidizes DOPA to form DOPA quinones. Therefore, it is essential to inhibit the activity of tyrosinase in the development of skin lightening agents that inhibit melanin production.

Tyrosinase related protein-1 (TRP-1) oxidizes DHICA (5,6-dihydroxy indole-2-carboxylicacid) produced by TRP-2 to produce indole-2-carboxylic acid (IQCA). Indicates. Therefore, whitening effect can be expected by inhibiting TRP-1 expression.

TRP-2 is an enzyme known as DOPA chromium tautomerase to produce DHICA (5,6-dihydroxy indole-2-carboxylic acid) using DOPA chromium. DHICA is oxidized by TRP-1 to produce indole-2-carboxylic acid (IQCA), which is dark brown. Therefore, whitening effect can be expected when TRP-2 expression is suppressed.

On the other hand, TNF-α, IL-1, etc. secreted from keratinocytes may act on the dermal dermal fibroblasts (Fibroblast) to increase the biosynthesis of MMP-1 (martrix metalloproteinase). Proteolytic enzymes, such as MMP-1, promote the breakdown of dermal collagen, creating skin wrinkles and aging.

1. Republic of Korea Patent Publication No. 10-2011-0041098 2. Korean Patent Publication No. 10-2016-0014321 3. Republic of Korea Patent Publication No. 10-1863722

In the present invention, in preparing a cosmetic composition, by prescribing only natural ingredients derived from chemicals as much as possible, skin elasticity containing the skin-friendly natural product extracts and fermented extracts having a nature-friendly, skin moisturizing effect, antioxidant action and whitening function An object of the present invention is to provide a cosmetic composition for improving and alleviating dark circles and a method of manufacturing the same.

Skin-elasticity improvement and dark circle relief cosmetic composition containing the skin-friendly natural product extract and fermentation extract of the present invention solved the above problems is purified water, moisturizing and nutrient supply function mixture A, wrinkle improvement and antioxidant function mixture B And, a skin moisturizing and formulation stabilizing function mixture C, a mixture D comprising a natural product extract and a fermentation extract, and a fragrance; 17.7-18.7 wt% of the mixture A, 1.2-1.5 wt% of the mixture B, 0.1-0.2 wt% of the mixture C, 0.07-0.09 wt% of the mixture D, 0.01-0.02 wt% of the fragrance, and the remaining amount of purified water Characterized in that.

Here, the mixture A is butylene glycol (Butylene Glycol), glycerin (Glycerin). Niacinamide, Bis-PEG-18 Methyl Ether Dimethyl Silane, Sodium Hyaluronate, Dimethicone, Sucrose distearate Distearate), the mixing ratio is characterized in that the mixture is mixed in a weight ratio of 8-9: 5-6: 2-3: 1-2: 1-2: 0.5-1: 0.2-0.5.

Here, the mixture B is phenoxyethanol, Hydrogenated Lecithin, Fiji-2M (PEG-2M), Methyl Gluceth-20, Safflower Osome (Carthamus) Tinctorius (Safflower) Oleosomes, Gluconolactone, Sodium Benzoate, Calcium Gluconate, 1,2-Hexanediol, Ethylhexylglycerin , PIG-60 Hydrogenated Castor Oil, Carbomer, Tromethamine, and the mixing ratio of 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 3: 0.5 to 1: 0.5 to 1 .

Here, the mixture C includes Xanthan Gum, Adenosine, and Rosemary Extract (Rosmarinus Officinalis (Rosemary) Extract), and the mixing ratio is mixed in a weight ratio of 5-6: 4-5: 1-3. It is characterized in that the mixture.

Here, the mixture D including the natural extract and fermented extract is Port Marigold extract (Calendula Officinalis Extract), wild geranium extract (Geranium Maculatum Extract), Matricaria extract (Chamomilla Recutita (Matricaria) Extract), camellia flower extract (Camellia Japonica Flower Extract), Lactobacillus / Soybean Ferment Extract, Saccharomyces / Viscum Album (Mistletoe), Saccharomyces / Imperata Cylindrica Root It includes, and the mixing ratio is characterized in that the mixture is mixed in a weight ratio of 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3.

In addition, the present invention provides a method for producing a cosmetic composition for skin elasticity improvement and dark circle relief containing a natural product extract and a fermented extract comprising the following steps.

Purified water, moisturizing and nourishing mixture A, anti-wrinkle and antioxidant functional mixture B, skin moisturizing and formulation stabilizing functional mixture C, mixture D including natural product extract and fermented extract, perfume, and then purified water A first step of adding the mixture A at a predetermined weight ratio and then mixing the mixture A for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer;

Adding the mixture B to the mixture of the first step, and then mixing the mixture B for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer;

Adding the mixture C to the mixture of the second step, and then mixing the mixture C for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer;

Adding the mixture D to the mixture of the third step, and then mixing the mixture D for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer; And

A fifth step of mixing the fragrance into the mixture of the fourth step and then mixing the mixture for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer;

Here, the input ratio of the mixtures added in each step is 17.7-18.7% by weight of mixture A, 1.2-1.5% by weight of mixture B, 0.1-0.2% by weight of mixture C, 0.07-0.09% by weight of mixture D, based on the total amount of mixture. , Mixed with 0.01 ~ 0.02% by weight and the remaining amount of purified water,

The mixture A is butylene glycol (Glycerin), glycerin (Glycerin). Niacinamide, Bis-PEG-18 Methyl Ether Dimethyl Silane, Sodium Hyaluronate, Dimethicone, Sucrose distearate Distearate), the mixing ratio is a mixture mixed in a weight ratio of 8: 5: 2: 1: 1: 0.5: 0.2,

The mixture B is phenoxyethanol, Hydrogenated Lecithin, PIG-2M, PEG-2M, Methyl Gluceth-20, Safflower Osome, Carthamus Tinctorius Safflower) Oleosomes, Gluconolactone, Sodium Benzoate, Calcium Gluconate, 1,2-Hexanediol, Ethylhexylglycerin, Blood Includes PEG-60 Hydrogenated Castor Oil, Carbomer, and Tromethamine, with a mixing ratio of 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 0.5: 0.5 mixture in a weight ratio of

The mixture C includes Xanthan Gum, Adenosine, and Rosemary Extract (Rosmarinus Officinalis (Rosemary) Extract), and the mixing ratio is a mixture mixed at a weight ratio of 5: 4: 1,

The mixture D including the natural extract and the fermented extract is Port Marigold Extract (Calendula Officinalis Extract), Wild Geranium Extract (Geranium Maculatum Extract), Matricaria Extract (Chamomilla Recutita (Matricaria) Extract), Camellia Flower Extract (Camellia) Japonica Flower Extract, Lactobacillus / Soybean Ferment Extract, Saccharomyces / Viscum Album (Mistletoe), Saccharomyces / Imperata Cylindrica Root , And the mixing ratio is a mixture mixed in a weight ratio of 1: 1: 1: 1: 1: 1: 1.

The skin antioxidant cosmetic composition containing the natural product extract and the fermented extract according to the present invention has a hyaluronic acid, which is processed into products such as ampules, and has the effect of making skin healthy as well as skin moisturizing when used.

In addition, the skin antioxidant cosmetic composition containing the natural extracts and fermented extracts according to the present invention contains brightening and wrinkled skin with bright skin and porcelain skin by containing niacin amide as a whitening functional ingredient and adenosine as a wrinkle improving functional ingredient. It has a changing effect.

In addition, the skin antioxidant cosmetic composition containing the natural extracts and fermented extracts according to the present invention contains natural herbal extracts and fermented extracts rich in protein, lactose and vitamins, making it skin-friendly and skin-safe. At the same time to secure the skin vitality has the effect of supplying nutrition to the skin.

In addition, the method for producing a skin antioxidant cosmetic composition containing a natural product extract and a fermented extract according to the present invention by providing only a natural ingredients derived by excluding the chemical components to provide a natural-friendly cosmetic composition, such as using the muffle The product can provide beneficial functional cosmetics for the skin that have the effect of maintaining / restoring healthy skin such as skin moisturizing, skin antioxidant action, and nutrition supply.

1 is a manufacturing process diagram showing an embodiment of a method for producing a skin antioxidant cosmetic composition containing a natural product extract and fermented extract of the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention is a natural-friendly cosmetic composition prepared with natural ingredients, which excludes chemical components as much as possible, as well as skin moisturizing, skin anti-oxidant action, excellent whitening function and skin-friendly natural extracts and fermented extracts, skin elasticity improvement and dark circles The first object of the present invention to provide a cosmetic composition for relaxation, and to provide a method for producing the cosmetic composition, the second object,

Skin-elasticity improvement and dark circle relief cosmetic composition containing a skin-friendly natural product extract and fermented extract according to the present invention to achieve the first object is purified water, moisturizing and nutrient supply function A, wrinkle improvement and antioxidant Characterized by comprising a functional mixture B, a skin moisturizing and formulation stabilizing functional mixture C, a mixture D comprising a natural product extract and a fermented extract, and a flavoring agent,

Preferably the cosmetic composition is based on the total amount of the composition, 17.7-18.7% by weight of the mixture A, 1.2-1.5% by weight of the mixture B, 0.1-0.2% by weight of the mixture C, 0.07-0.09% by weight of the mixture D, 0.01-0.02% by weight of the perfume And the remaining amount of purified water.

According to the invention, preferably the mixture A is butylene glycol (Butylene Glycol), glycerin (Glycerin). Niacinamide, Bis-PEG-18 Methyl Ether Dimethyl Silane, Sodium Hyaluronate, Dimethicone, Sucrose distearate Distearate), and the mixing ratio is a mixture of 8: 5: 2: 1: 1: 0.5: 0.2 by weight.

According to the invention, preferably the mixture B is phenoxyethanol (Phenoxyethanol), Hydrogenated Lecithin, Fiji-2M (PEG-2M), Methyl Gluceth-20 (Methyl Gluceth-20), Safflower Oleosomes (Carthamus Tinctorius (Safflower) Oleosomes), Gluconolactone, Sodium Benzoate, Calcium Gluconate, 1,2-Hexanediol, Ethyl Hexylglycerin (Ethylhexylglycerin), PIG-60 Hydrogenated Castor Oil (PEG-60 Hydrogenated Castor Oil), Carbomer, Tromethamine (Tromethamine) Use a mixture in a weight ratio of 1: 1: 1: 1: 1: 1: 1: 1: 0.5: 0.5.

According to the invention, preferably the mixture C comprises Xanthan Gum, Adenosine, Rosemary Extract (Rosmarinus Officinalis (Rosemary) Extract), the mixing ratio is 5: 4: 1 Use a mixture.

According to the present invention, preferably, the mixture D including the natural product extract and the fermented extract is Port Marigold Extract (Calendula Officinalis Extract), Wild Geranium Extract (Geranium Maculatum Extract), Matricaria Extract (Chamomilla Recutita (Matricaria) Extract) , Camellia Japonica Flower Extract, Lactobacillus / Soybean Ferment Extract, Saccharomyces / Viscum Album (Mistletoe), Saccharomyces / Saccharomyces / Imperata Cylindrica Root), and the mixing ratio is a mixture of 1: 1: 1: 1: 1: 1 by weight.

The method for preparing the skin elasticity improvement and dark circle relief cosmetic composition containing the natural product extract and the fermented extract according to the present invention, which has achieved the second object, will be described in detail with reference to FIG. 1.

First, as an ingredient of the ampoule, purified water, moisturizing and nourishing mixture A, wrinkle improvement and antioxidant functional mixture B, skin moisturizing and formulation stabilizing mixture C, mixture D including natural extract and fermented extract, Prepare spices.

According to the present invention, the mixture A is prepared by mixing and preparing a functional material that functions to moisturize and prevent the skin from drying, and the mixture B is a skin wrinkle improvement and skin antioxidant, anti-inflammatory, conditioning function The mixture C is prepared by preparing a functional raw material having a mixture, and the mixture C is prepared by mixing the functional raw materials having a function of preventing skin moisturizing and stabilization and decay of the formulation, and anticipation, the mixture D is a skin softening effect and antioxidant, skin It is characterized by consisting of natural extracts and fermented extracts having the function of enhancing regeneration and skin absorption.

Method for producing a skin antioxidant cosmetic composition containing the natural product extract and fermented extract of the present invention using the prepared materials,

After adding purified water to the mixing vessel (VESSEL), the mixture A in a predetermined weight ratio, the first step of mixing for 5-7 minutes at 3,000 ~ 4,000rpm with an AGI Mixer; and,

Injecting the mixture B to the mixture of the first step, the second step of mixing for 5-7 minutes at 3,000 ~ 4,000rpm with an AGI Mixer;

Adding the mixture C to the mixture of the second step, and then mixing the mixture C for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer;

Adding the mixture D to the mixture of the third step, and then mixing the mixture D for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer; And

The fifth step of mixing the fragrance into the mixture of the fourth step, 5-7 minutes at 3,000 ~ 4,000rpm with an AGI Mixer; characterized in that it comprises a.

At this time, the rosemary extract constituting the mixture C corresponds to a natural product extract, should be configured in the mixture D of the fourth step. The rosemary extract is characterized by being well soluble in xanthan gum and adenosine, and constituted mixture C with xanthan gum and adenosine.

In addition, the natural product extract and the fermentation extract constituting the mixture D may have a problem that the efficacy effect is lowered when stirring for a long time with the AGI MIXER, the mixture was added to the step before adding the fragrance to complete the present invention. .

According to the present invention, the rotation speed of the azimuth mixer in the first to fifth steps is homogeneous mixing, in order to suppress the generation and precipitation of bubbles in the mixing process, the stirring speed of the azimixer is 3000 ~ 4000rpm It is desirable not to go out of range. If the rotational speed of the azimixer is less than 3,000 rpm, there is a problem that the mixture does not mix evenly and may be precipitated after production, and if it exceeds 4,000 rpm, a large amount of bubbles are generated to destabilize the properties and formulation. There is a problem happening.

In addition, if the mixing time is less than 5 minutes, there is a problem that the mixture is not mixed well, if more than 7 minutes there is a problem that the destabilization of the properties and formulation.

According to the present invention, the raw materials constituting the mixture A preferably added to the first step is to moisturize and prevent the drying of the skin and to supply nutrition to the skin, the mixture A is butylene glycol (Butylene Glycol), Glycerin. Niacinamide, Bis-PEG-18 Methyl Ether Dimethyl Silane, Sodium Hyaluronate, Dimethicone, Sucrose distearate Distearate), and the mixing ratio is preferably a mixture mixed at a weight ratio of 8-9: 5-6: 2-3: 1-2: 1-2: 0.5-1: 0.2-0.5. . More preferably, the mixing ratio is a mixture used in a weight ratio of 8: 5: 2: 1: 1: 0.5: 0.2.

According to the present invention, the raw materials constituting the mixture B, which is preferably added to the second step, serves to prevent stabilization, decay, and acidification of skin moisturizing and formulation, and the mixture B is phenoxyethanol (Phenoxyethanol ), Hydrogenated Lecithin, PIG-2M, Methyl Gluceth-20, Carthamus Tinctorius (Safflower) Oleosomes, Glunolactone ( Gluconolactone, Sodium Benzoate, Calcium Gluconate, 1,2-Hexanediol, Ethylhexylglycerin, Fiji-60 Hydrogenated Castor Oil Using a mixture containing PEG-60 Hydrogenated Castor Oil, Carbomer, Tromethamine,

The mixing ratio is 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 0.5 ~ 1 It is preferable that it is a mixture mixed by the weight ratio of: 0.5-1. More preferably, the mixing ratio is a mixture in a weight ratio of 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 0.5: 0.5.

According to the present invention, the mixture C preferably added to the third step uses a mixture including Xanthan Gum, Adenosine, and Rosemary Extract (Rosmarinus Officinalis (Rosemary) Extract). It is preferable that it is a mixture mixed by the weight ratio of 5-6: 4-5: 1-3. More preferably, the mixing ratio is a mixture used in a weight ratio of 5: 4: 1.

The raw materials constituting the mixture C have wrinkle improvement, skin antioxidant, anti-inflammatory and conditioning functions. At this time, the rosemary extract is a natural product extract having anti-wrinkle and skin antioxidant, anti-inflammatory, and conditioning functions such as xanthan gum and adenosine, and is well soluble in the xanthan gum and adenosine, so that the inventors of the present invention constitute the mixture C. Used.

At this time, the rosemary extract constituting the mixture C prepared dried rosemary, the rosemary and purified water in a weight ratio of 1:40 ~ 1: 100 in a hot water extract, 360-420 minutes at a temperature 90 ~ 95 ℃ After the extraction, the filtrate obtained by filtration with a filter may be used as an extract obtained by sterilizing with a sterilizer, or a viscous concentrate obtained by concentrating the extract obtained by filtration for a certain time may be used.

According to the present invention, the mixture D including the natural product extract and the fermented extract, which is added to the fourth step, is Port Marigold Extract (Calendula Officinalis Extract), Wild Geranium Extract (Geranium Maculatum Extract), Matricaria Extract (Chamomilla Recutita) (Matricaria) Extract, Camellia Japonica Flower Extract, Lactobacillus / Soybean Ferment Extract, Saccharomyces / Viscum Album (Mistletoe), Yeast / Bird Root A mixture containing fermented extract (Saccharomyces / Imperata Cylindrica Root) is used, and the mixing ratio is 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3 by weight. It is preferable to use a mixed mixture, and more preferably, the mixing ratio is a mixture in a weight ratio of 1: 1: 1: 1: 1: 1: 1.

At this time, the port marigold extract, wild geranium extract, Matricaria extract, camellia flower extract may be prepared using a conventional method known in the art, that is, using a conventional solvent under a conventional high temperature and pressure. have. As an extraction solvent that can be used for the preparation of the extracts, for example, water, lower alcohol, acetone, ethyl acetate, 1,3 butylene glycol may be used alone or in combination. The suitable amount of the extraction solvent can be appropriately adjusted according to the dry weight of the extraction raw materials, and can be selected and used as an extraction method, such as hot water extraction, cold sediment extraction, ultrasonic extraction, extraction temperature, extraction frequency, etc. Are not particularly limited.

In the present invention, the present invention was completed by adopting a hot water extraction method to obtain the extracts, and the extracts were prepared from each of the dried extract raw materials (port marigold, wild geranium, matricaria, camellia flower), Each raw material and purified water were put into a hot water extractor at a weight ratio of 1:40 to 1: 100, extracted for 360 to 420 minutes at a temperature of 90 to 95 ° C, and filtered through a filter to sterilize the filtrate obtained by a sterilizer. Alternatively, a concentrated solution obtained by concentrating the filtrate obtained by sterilization for a predetermined time may be used.

According to the present invention, the fermentation method for obtaining the Lactobacillus / soybean fermentation extract, yeast / mistletoe fermentation extract, yeast / strip root fermentation extract can also be carried out according to the conventional methods known in the art. That is, it can be cultured under conventional conditions and aerobic or usually anaerobic conditions depending on each strain used. In addition, an additional carbon source and / or pH adjuster may be added to further activate the culture of the fermentation bacteria.

Preferably, the Lactobacillus / soybean fermented extract is prepared by washing the beans with purified water, dried, and powdered it, adding purified water or distilled water to the soybean powder in a weight ratio of 1: 5-7 and at 90-95 ℃ After extraction for 3 to 10 hours, the first filtration with a filter cloth, and then filtered again with a filtration filter of 0.4 ~ 0.5 ㎛ level to obtain a filtrate, the filtrate is sterilized to obtain a final soybean extract, to the soybean extract Lactobacillus bacteria were inoculated.

At this time, the Lactobacillus bacteria is selected from the group consisting of Lactobacillus brevis, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus ashdophyllus, Lactobacillus elbeticus and Lactobacillus delbrueki subspecies Bulgaricus It is preferable to use one or more, but is not limited thereto.

The Lactobacillus bacteria are prepared in an aqueous solution by diluting the cultured bacteria in tertiary distilled water at a level of 1 to 2% (V / V), and the aqueous solution is mixed with a soybean extract in a weight ratio of 1: 100, in a clean room After culturing for 2-3 days at a temperature of 36 ° C., the culture solution was first filtered through centrifugation and a filtration filter of 0.4 to 0.5 μm, and after removing the culture and debris, the mixture was filtered using a filtration membrane of 0.3 μm or less. Tea filtered. The filtrate was concentrated using a reduced pressure distillation to obtain Lactobacillus / soybean fermented extract.

At this time, as the type of soybean used is any one or more selected from the group consisting of soybean, black beans or rat eye, preferably fermented extract obtained by fermenting soybeans.

The yeast / mistletoe fermentation extract is washed with dried mistletoe with purified water, purified water or distilled water was added to the mistletoe prepared in finely divided by weight ratio of 1: 5-7 and extracted at 90-95 ℃ for 3-10 hours, filtered through a filter cloth After preparing the mistletoe extract prepared by sterilizing the filtrate filtered with a 0.4 ~ 0.5㎛ filtration filter, the mistletoe extract was inoculated with yeast.

At this time, the yeast was used a natural yeast, the natural yeast was aged for 7 days at room temperature in a sterile chamber without direct sunlight, and then filtered by a filter to remove the residue was used.

After yeast inoculation, fermentation is inoculated into the mistletoe extract at a weight ratio level of 100: 0.5 to 1, mixed, fermented in a clean room at 36 to 37 ° C. for 3 to 7 days, and the fermentation is centrifuged and 0.4 to After primary filtration with a filter of 0.5㎛ level, after removing the culture bacteria and debris, secondary filtration using a filter membrane of 0.3㎛ or less. This filtrate was concentrated using a reduced pressure distillation to obtain a yeast / mistletoe fermentation extract obtained.

The yeast / strip root fermentation extract is used to prepare the extract in the same way as the yeast / mistletoe fermentation extract.

The mistletoe (Mistletoe, Viscum album) is a plant belonging to the family Mistletoe is known to have 1,500 species of 30 genera throughout the world. Mistletoe, which is used as a medicament among several species of mistletoe, is a mistletoe of the genus Viscum. In particular, the European mistletoe Viscum album loranthacea has already been used as an anticancer and anticancer supplement in Europe. The Korean mistletoe is a species of Viscum album coloratum that differs from the European mistletoe. It is a parasitic plant that hosts various trees such as oak, pear, alder, apple, hackberry, pine and spruce. In herbal medicine, the herb life has been used in soothing, pain, pain, low back pain, high blood pressure, small swelling, prevention of miscarriage, toothache, pain. In addition, Lactobacillus fermenting mistletoe is known in Europe, Lactobacillus plantarum (Lactobacillus plantarum), it is reported that the mistletoe fermented with the lactic acid bacteria has an immune enhancing effect to stimulate the humoral and cellular immune system. Mistletoe fermentation by lactic acid bacteria is known to reduce the direct toxic effect of mistletoe on cells while maintaining the immunostimulatory activity of mistletoe.

The band root (white hair root) refers to the removal of scaly leaves as fine roots and rhizomes of the rice band (Imperata cylindrica Beauvois var. Koenigii Durand et Schinz) in the family of rice in Korea. In Japan, it refers to the hair root (Imperata cylindrica Beauvois: 茅根), and in China, it refers to the white hair (Imperata cylindrica Beauvois var. Major C.E Hubbard: 白茅).

The hair roots were named long hairs because they were like long spears. This medicine is odorless and tastes tasteless at first, but a bit sweet and cold afterwards.

Hair roots are used for hemostasis caused by blood fever, nosebleeds, urine bleeding, bleeding, uterine bleeding, and menstrual irregularities. Jaundice, stomach fever, waste heat due to seawater, asthma is also used as a pharmacological action has been reported to be excellent in diuretic, antibacterial action.

Appearance is thin, long columnar, the outer side is yellowish white, there are some longitudinal wrinkles, nodes and sometimes divided. Folded face is fibrous, transverse section is irregular circular, thickness of cortex is slightly thinner than the diameter of central column, number of tissues is sometimes empty. Other names include White Hair Root, White Root, White Hair Root, White Hair, Female Root, Jigwan, Ji-Geun, and Geun-Myung-Geun名 茹 根), and strip roots .

According to the present invention, the fragrance introduced in the fifth step may be used as long as it is usually used in cosmetics.

According to the invention, preferably in the above manufacturing process, the input ratio of the mixtures added in each step is 17.7 ~ 18.7% by weight of mixture A, 1.2 ~ 1.5% by weight of mixture B based on the total amount of the mixture completed in the fifth step %, Mixture C 0.1-0.2% by weight, Mixture D 0.07-0.09% by weight, fragrance 0.01-0.02% by weight and the residual amount of purified water are preferably added to mix, more preferably, the mixture A 17.7 It is best to add and mix to include the weight%, 1.2% by weight of mixture B, 0.1% by weight of mixture C, 0.07% by weight of mixture D, 0.01% by weight of perfume and the remaining amount of purified water.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. only. The following examples are not intended to limit the present invention as an example for explaining the present invention, and those skilled in the art can be modified as much as possible without departing from the contents of the claims.

Example 1

To prepare each raw material as shown in Table 1 below, using the raw material to prepare a cosmetic composition based on the process diagram as shown in Figure 1, using the composition to revitalize into a cosmetic sample for skin (revitalizing ampoule) An ampule was prepared and prepared.

Rosemary extract constituting the mixture C of Table 1 is to prepare a dried rosemary, the rosemary and purified water to a hot water extractor in a weight ratio of 1:40 ~ 100, extracted at a temperature of 90 ~ 95 ℃ 360 ~ 420 minutes The filtrate obtained by sterilizing the filtrate obtained by filtration with a filter was prepared as the extract and used.

Fort marigold, wild geranium, Matricaria, camellia flower extract constituting the mixture D of Table 1, respectively prepared dried extract raw materials (potmarigold, wild geranium, Matricaria, camellia flower), Each of the extracted raw materials and purified water were put into a hot water extractor at a weight ratio of 1:40 to 100, extracted for 360 to 420 minutes at a temperature of 90 to 95 ° C., and the filtrate obtained by filtration with a filter was sterilized by a sterilizer. The filtrate was used as the extract.

In addition, the Lactobacillus / soybean fermentation extract constituting the mixture D is prepared by washing the soybeans with purified water, dried, and powdered it, adding purified water to the soybean powder in a weight ratio of 1: 5-7 and 90 After extraction for 3-10 hours at ~ 95 ℃, the first filtration with a filter cloth, and then filtered secondly with a filter of 0.4 ~ 0.5 ㎛ level to obtain a filtrate, the filtrate is sterilized to obtain a final soybean extract , Soybean extract was inoculated with Lactobacillus bacteria. At this time, the Lactobacillus bacteria are prepared in an aqueous solution by diluting the cultured bacteria to the level of 1 ~ 2% (V / V) in the tertiary distilled water, the aqueous solution 1: 100 (aqueous solution: soybeans) Extract)), and after culturing for 2-3 days at a temperature of 36 ℃ in a sterile chamber, centrifugation and primary filtration with a filter of 0.4 ~ 0.5 ㎛ level, after removing the culture and debris It filtered secondaryly using the filtration membrane of 0.3 micrometer or less. The filtrate was concentrated using a reduced pressure distillation to obtain Lactobacillus / soybean fermented extract.

The yeast / mistletoe fermentation extract is washed with dried mistletoe with purified water, and added to the mistletoe prepared by finely divided water at a weight ratio of 1: 5-7 and extracted for 3-10 hours at 90-95 ℃, filtered with a filter cloth, 0.4 Mistletoe extract prepared by sterilizing the filtrate filtered with a ~ 0.5㎛ level filter, and then the mistletoe extract was inoculated with yeast. After yeast inoculation, fermentation is inoculated into the mistletoe extract at a weight ratio level of 100: 0.5 to 1, mixed, fermented in a clean room at 36 to 37 ° C. for 3 to 7 days, and the fermentation is centrifuged and 0.4 to After primary filtration with a filter of 0.5㎛ level, after removing the culture bacteria and debris, secondary filtration using a filter membrane of 0.3㎛ or less. This filtrate was concentrated using a reduced pressure distillation to obtain a yeast / mistletoe fermentation extract obtained.

At this time, the yeast was used a natural yeast, the natural yeast was aged for 7 days at room temperature in a sterile chamber without direct sunlight, and then filtered by a filter to remove the residue was used.

The yeast / strip root fermentation extract was prepared by washing the dried strips with purified water, then chopped, and the prepared strips were prepared by using the same method as the yeast / mistletoe fermentation extract.

[Comparative Example]

Revitalizing ampoule The product Revitalizing C I2PL Corrector Ampoule manufactured by Emflow A was prepared.

The revitalizing muffle prepared in Example 1 was divided into three samples, the product of manufacturer A of Comparative Example was prepared, divided into two groups of 40 subjects each, and the skin elasticity improvement and dark circle relaxation test was performed as follows. It was compared and tested by the test method.

<Skin Elasticity Improvement Test>

▶ Subject Selection Criteria

1) The person in charge of the study or the person authorized by the study supervisor has fully explained the matter to be informed of the subject, and voluntarily completed and signed the consent form.

2) Healthy person who does not have grade and chronic physical disease including skin disease

3) A person who can follow up during the test period

▶ Exclusion Criteria for Subject Selection: The following subjects are excluded from subjects by interviewing applicants.

1) Pregnant or lactating women and women who may be pregnant

2) Those who use steroid-containing skin external preparation for more than 1 month to treat skin disease

3) Person who has not passed 6 months after taking the same test

4) people with sensitive or irritable skin

5) Those who have skin abnormalities such as spots, acne, erythema, and capillary dilatation on the test site.

6) Those who used the same or similar efficacy cosmetics and medicines on the test site within 3 months before starting the study

7) Those who received the procedure at the test site within 6 months before the start of the study

8) Others who are deemed unsuitable for the examination at the judgment of the person in charge of the examination.

▶ Test site: Whole face including eyes (measurement area)

▶ Application of test substance: A person in charge of the test or the test subject is applied to the test site

                  The frequency of application is morning, evening, application according to usage and dosage

▶ Test place: No air movement, no direct sunlight, constant temperature and humidity conditions (20 ~ 24 ℃, 40 ~ 60% RH)

             Subjects should take skin stability at the test site for at least 30 minutes, expose the area to be measured, and measure

▶ Measurement: Negative pressure applied to the skin with Cytometer® or Dermaflex® to measure the extent to which it is restored.

<Dark Circle Relaxation Test>

▶ Subject Selection Criteria

1) vascular

-. Vascular dark circles: Dark circles seen under the eyes are either red or purple, and the dark circles seen through the wood lamp are not visible.

-. Pigmented dark circles: Dark circles are brown or grayish, and are clearly visible when viewed through a wood lamp.

2) A person who understands the purpose and contents of the examination and expresses his / her willingness to participate.

3) A person who understands possible adverse reactions and signs a written consent, and can follow up during the test period.

▶ Test site: Selecting dark circles under the eyes

▶ Application of test substance: A person in charge of the test or the test subject is applied to the test site

                  The frequency of application is morning, evening, application according to usage and dosage

▶ Test place: No air movement, no direct sunlight, constant temperature and humidity conditions (20 ~ 24 ℃, 40 ~ 60% RH)

             Subjects should take skin stability at the test site for at least 30 minutes, expose the area to be measured, and measure

▶ Measurement:

1) Determination of melanin in skin

   -. Measure with Mexameter MX18 instrument

   -. measuring melaini index changes

2) Skin brightness measurement

   -. Measure with Chromameter C-40 instrument

3) Skin color measurement

   -. High resolution digital camera (measured with Visa-CR (Canfield Imaging System) instrument

Meanwhile, the skin irritation test was simultaneously performed on the subjects who participated in each of the skin elasticity improvement and dark circle relaxation tests, and the test evaluated the skin stability of the product through visual observation and Q & A of the subject after using the sample product. It was. The skin stability evaluation was evaluated by dividing the subjective stimulus and the objective stimulus by observation of the subject.

In addition, each test subject was divided into 10 persons, and the sample 1, the sample 2, the sample 3, and the comparative sample were used for the test subject group, respectively.

<Test Results>

The results for the skin elasticity improvement and dark circle relaxation test and the results for the skin stability test are shown in Tables 2 and 3 below.

division Example Comparative example Sample 1 Sample 2 Sample 3 Skin elasticity improvement (%) 57 56 52 31 Dark Circle Relaxation (%) 35 33 32 22

The measured values in Table 1 above represent the values of ΔR8 measured by the measuring instrument, and are average values of R8 (8 weeks) to R8 (0 weeks). Is the value in percentile.

In this case, R8 is the average skin elasticity, the average skin lightening degree (1 minus dark circles).

As a result of Table 1, it can be seen that the skin elasticity improvement in the samples of the Example has an improvement effect of 55.0% on average, and the dark circle relaxation has a 33.3% relaxation effect.

On the other hand, the sample of the comparative example was found to have a skin elasticity improvement of 31%, the dark circle relaxation was found to have a 22% relaxation effect.

Compared to this, it can be seen that the sample of the Example showed an effect of improving skin elasticity by 77.4% on average compared to the sample of Comparative Example, and the dark circle relaxation showed an effect of 51.5% on average. .

division Sensory evaluation results Example Comparative example Sample 1 Sample 2 Sample 3 Subjective
Irritation itch 0 0 0 0 I'm sick. 0 0 0 0 It's hot. 0 0 0 0 Tingling 0 0 0 0 Stiff 0 0 0 0 Pull. 0 0 0 One Objective
Stimulus evaluation Erythema 0 0 0 0 edema 0 0 0 0 Inseol 0 0 0 0 Podrakji 0 0 0 0 Other 0 0 0 0 Total Auxiliaries 0 0 0 One

As a result of Table 3, the skin irritation symptoms such as erythema observed with the naked eye did not appear in each of the ten subjects, and thus it was confirmed that the skin was safe.

Claims (7)

Purified water, a moisturizing and nourishing mixture A, a wrinkle improving and antioxidant functional mixture B, a skin moisturizing and formulation stabilizing functional mixture C, a mixture D containing natural extracts and fermented extracts and a flavoring agent; 17.7-18.7 wt% of the mixture A, 1.2-1.5 wt% of the mixture B, 0.1-0.2 wt% of the mixture C, 0.07-0.09 wt% of the mixture D, 0.01-0.02 wt% of the fragrance, and the remaining amount of purified water By
The mixture A is butylene glycol (Butylene Glycol), glycerin (Glycerin), niacinamide (Niacinamide), bis-PG-18 Methyl Ether Dimethyl Silane, sodium hyalurotate ( Sodium Hyaluronate, Dimethicone and Sucrose Distearate, the mixing ratios are 8-9: 5-6: 2-3: 1-2: 1-2: 0.5-1: 1: A mixture of 0.2 to 0.5 by weight, the mixture B is phenoxyethanol (Phenoxyethanol), Hydrogenated Lecithin, Fiji-2M (PEG-2M), Methyl Gluceth-20 20), Carthamus Tinctorius (Safflower) Oleosomes, Gluconolactone, Sodium Benzoate, Calcium Gluconate, 1,2-hexanediol (1,2- Hexanediol), Ethylhexylglycerin, PIG-60 Hydrogenated Castor Oil, Contains Carbomer and Tromethamine, and the mixing ratio is 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 3: A mixture of 1 to 3: 1 to 3: 1 to 3: 0.5 to 1: 0.5 to 1 by weight ratio, the mixture C is Xanthan Gum, Adenosine and Rosemary Extract (Rosemaryus Officinalis) ), And the mixing ratio is a mixture of 5 to 6: 4 to 6: 1 to 3 by weight, the mixture D containing the natural extract and fermented extract is Calendula Officinalis Extract (Calendula Officinalis Extract) Fermented with Lactobacillus / Soybean Ferment Extract inoculated with Wild Geranium Extract, Geranium Maculatum Extract, Chamomilla Recutita (Matricaria) Extract, Camellia Japonica Flower Extract, and Lactobacillus ), Mistletoe fermentation extract (Saccharomyces / Viscum Album (Mistletoe)) fermented by inoculation with yeast and strip root fermented by inoculation with yeast Saccharomyces / Imperata Cylindrica Root, and the mixing ratio is 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3 Cosmetic composition for skin elasticity improvement and dark circle relief containing natural product extract and fermented extract, characterized in that.
delete delete delete delete Purified water, moisturizing and nourishing function mixture A, anti-wrinkle and antioxidant function mixture B, skin moisturizing and formulation stabilizing function mixture C, mixture D including natural product extract and fermentation extract, and fragrance were prepared, respectively. Adding the mixture A to the mixer, and then mixing the mixture A, and then mixing the mixture A for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer;
Adding the mixture B to the mixture of the first step, and then mixing the mixture B for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer;
Adding the mixture C to the mixture of the second step, and then mixing the mixture C for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer;
Adding the mixture D to the mixture of the third step, and then mixing the mixture D for 5 to 7 minutes at 3,000 to 4,000 rpm with an AGI Mixer; And
After the injecting the fragrance to the mixture of the fourth step, a fifth step of mixing for 5-7 minutes at 3,000 ~ 4,000rpm with an AGI Mixer;
The mixing ratio of the mixtures added in each step is 17.7-18.7 wt% of mixture A, 1.2-1.5 wt% of mixture B, 0.1-0.2 wt% of mixture C, 0.07-0.09 wt% of mixture D, flavoring It is mixed by adding 0.01 ~ 0.02% by weight and the remaining amount of purified water,
The mixture A is butylene glycol (Butylene Glycol), glycerin (Glycerin). Niacinamide, Bis-PEG-18 Methyl Ether Dimethyl Silane, Sodium Hyaluronate, Dimethicone, Sucrose Distearate Distearate), the mixing ratio is a mixture mixed in a weight ratio of 8: 5: 2: 1: 1: 0.5: 0.2,
The mixture B is phenoxyethanol, Hydrogenated Lecithin, PHY-2M (PEG-2M), Methyl Gluceth-20, Safflower Osome (Carthamus Tinctorius ( Safflower) Oleosomes, Gluconolactone, Sodium Benzoate, Calcium Gluconate, 1,2-Hexanediol, Ethylhexylglycerin, Blood Includes PEG-60 Hydrogenated Castor Oil, Carbomer, and Tromethamine, with a mixing ratio of 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 0.5: 0.5 mixture in a weight ratio of
The mixture C includes Xanthan Gum, Adenosine, and Rosemary Extract (Rosmarinus Officinalis (Rosemary) Extract), and the mixing ratio is a mixture mixed in a weight ratio of 5: 4: 1,
The mixture D including the natural extract and the fermented extract is Port Marigold Extract (Calendula Officinalis Extract), Wild Geranium Extract (Geranium Maculatum Extract), Matricaria Extract (Chamomilla Recutita (Matricaria) Extract), Camellia Flower Extract (Camellia) Japonica Flower Extract), Lactobacillus / Soybean Ferment Extract inoculated with Lactobacillus, Mistletoe Fermentation Extract (Saccharomyces / Viscum Album (Mistletoe)) inoculated with yeast, and strip root fermented with yeast It contains fermented extract (Saccharomyces / Imperata Cylindrica Root), the mixing ratio of the skin elasticity containing the natural product extract and fermented extract, characterized in that the mixture is a mixture of weight ratio of 1: 1: 1: 1: 1: 1: 1 Method for producing a cosmetic composition for improving and alleviating dark circles. delete

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