KR20020084429A - Cosmetic composition containing a Rosa davurica Pall extracts for application to the skin - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Rosa davurica PALL. extract is provided which has an effect on inhibiting the formation of a melanin pigment, helping wound healing and promoting collagen formation. CONSTITUTION: Flowers, fruits and roots of Rosa davurica PALL. are extracted 1 to 15 times with a solvent containing water, acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane and C1-4 lower alcohol at 50 to 95deg.C for 1 to 20hr in an extractor with a cooling condenser or soaking at 5 to 45deg.C for 1 to 15 days and then filtering with a filter cloth.

Description

Cosmetic composition containing bio-extract extract {Cosmetic composition containing a Rosa davurica Pall extracts for application to the skin}

The present invention relates to a composition for skin having a skin whitening, wrinkle improvement, elasticity enhancement, wound healing action containing a collagen synthesis promoter or melanin production inhibitor. The bio-ear extract of the present invention has an excellent effect of promoting collagen synthesis, which promotes collagen synthesis of fibroblasts, and is excellent in inhibiting wound healing or melanogenesis. In addition, the composition for skin containing bio-ear extract extracts brightens the skin color when applied to the human body and has an excellent wrinkle-improving effect.

Human skin is subject to severe external environmental stress such as ultraviolet rays, pollution and physical stimuli, and as the process of endogenous aging progresses, melanin production is increased and collagen is broken down, which can cause serious cosmetic problems. First, collagen, which has the most relation to wrinkle formation, is a major substrate produced in fibroblasts of the dermal layer of skin and is an important protein present in extracellular epilepsy and accounting for 30% of the total weight of biological proteins. Its main functions are known as mechanical firmness of skin, resistance of connective tissue and adhesion of tissue, support of cell adhesion, induction of cell division and differentiation. The collagen is reduced by aging and photoaging due to ultraviolet rays. In general, collagen is reduced by 65% compared to 20 years old, and the thickness of the skin becomes thin. This phenomenon is closely related to wrinkles of the skin. Increasing the composition of the dermal matrix by promoting collagen synthesis in the skin can be expected to improve wrinkles, increase elasticity, and heal wounds. Collagen synthesis promoters are commonly known as retinoic acid, transforming growth factor (TGF), and other placenta extracts (JP8-231370), betulinic acid (JP8-208424), and chlorella extracts. JP 9-40523, JP10-36283), etc., include Korean Yulpi extract (published patent 1999-0039012), yellowish white, clove carrot extract (published patent 1999-0075512), landing, earthenware, baekgang sleep extract (published patent 2000-0013588) Etc. are known. However, retinoic acid is unstable and has safety problems such as irritation and redness when applied to the skin, so it can be applied only as a prescription drug of doctors. Peptide factors are large in size, so it is difficult to absorb skin, and other plant extracts are ineffective, odor, color There were problems in the formulation due to such problems.

On the other hand, the color of the skin is determined by the black pigment called melanin produced by the pigment cells (Melanocyte) in the basement layer of the epidermis, and the imelanin pigment is widely distributed to the surrounding keratinocytes through the dendritic processes of the pigment cells. Will determine the color. Skin pigmentation disorders such as blemishes, freckles, and senile plaques are caused by abnormally activated melanin production in pigmented cells resulting in excessive production deposition. This hyperpigmentation can be a serious mental burden from a cosmetic point of view that can interfere with normal social activities. In addition, Asian women have long preferred white and fair skin, and have made it an important standard of beauty.

Due to these social demands, there have been many developments of whitening agents for treating skin pigmentation and satisfying cosmetic needs. Although substances with tyrosinase inhibitory activity, such as ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione and derivatives thereof, have been formulated into cosmetics or medicines, cosmetics and medicines with whitening effects have been provided. However, its use is limited due to the stability of the formulation when formulated in cosmetics or pharmaceutical products and the insufficient whitening effect. In addition to these whitening substances, research has been carried out to find whitening active ingredients in natural products, especially plants, among them baekbaekpi (Japanese Patent Publication No. 55-44375, Cattle 64-26507, Cattle 64-83009, Pyeong 1-25687, Pyeong 5) -139950 and Korean Patent Laid-Open No. 92-002109, 97-021273), Licorice (Japanese Patent Laid-Open No. 60-214721, Small 60-214728, Small 63-23809, Small 64-63506, Hei 1-149706 and Korean Laid-Open Patent 92- 002109, 97-025601), Peony (Japanese Patent Laid-Open No. 61-246109 and Korean Patent Laid-Open No. 92-002111), Cinnamon (Japanese Laid-Open Patent No. 63-30403, Hep. 5-139954), Gosam (Japanese Laid-Open Patent No. 64-26507) And Korean Patent Publication No. 92-002110), Brown root (Japanese Patent Publication No. 60-214727, Small 64-16709), Angelica (Japanese Patent Publication No. 56-92211, Hep. 4-26610), Bark skin (Japanese Patent Publication No. 60-214721) , SO 61-50915, SO 61-246109), Banha (Japanese Patent Laid-Open No. 2-207028, Korean Patent Publication No. 96-033442), Aloe (Japanese Laid-Open Patent No. 52-44375, Hei 2-207030, Hei 5-139950) Many plant extracts, It has been found that herbal extracts and the like act on tyrosinase to inhibit melanin production, but they also have many problems in using them beyond effective concentrations in cosmetics and pharmaceuticals in terms of safety, stability, and discoloration. The situation is not working.

Rosa davurica Pall is a perennial plant belonging to the family Rosaceae and is a deciduous broad-leaved shrub distributed in Japan, Manchuria and Siberia. The roots and flowers of fresh heat are used for dry stomach and sheep's landscaping. It is a useful medicinal material for the treatment of dyspepsia, anticipated radiation, stomach pain, and menstrual dysmenorrhea. Fruits have been reported to be 10-30 times higher in vitamin C (ascorbic acid) than lemon and 8-10 times higher in beta-carotene than carrots. Also, vitamin B2, vitamin K, pectin and tannin It is contained.

The present inventors have discovered that the bio-ear ear extract, which is known to contain a large amount of useful skin components such as vitamin C and catechin, has a safe and effective wrinkle improvement and skin whitening effect and has completed the formulation containing the skin cosmetic composition. To provide.

The present invention relates to a cosmetic composition for improving skin wrinkles, skin whitening and wound healing, containing bio-ear extract (extract fruit, leaves or roots with water or organic solvent or a mixture of water and organic solvent).

Rosa davurica Pall is a perennial plant belonging to the family Rosaceae, and is a lateral bowel shrub distributed in Korea, North and South Korea, Japan, Manchuria, and Siberia. The height is 1 ~ 1.5m and the stem is straight, reddish brown and thorny. The leaves are divided into right upper bilobites, oval or elliptical, pointed at both ends, 1 ~ 3cm long, serrated at the edge, and occupied at the back. Flowers are rose-colored and fragrant. Fruit is spherical and ripens in yellow in September. In the private sector, fruits are used for food, and the roots have been used for diabetes and stomach pain. In the private sector, it has also been used as a vitamin deficiency, increased resistance to various diseases, and as a protective agent for liver function.

Investigation of the content of vitamin C, by the part of bio-deciduous tree at Seoul National University, leaves (September harvest leaf 1115.5mg / 100g, November harvest leaf: 1973.9mg / 100g) fruit (714.6mg / 100g), root ( 138.8mg / 100g) and stem (136.3mg / 100g) in order to contain vitamin C. (Shinguk outside the five people, "collection area and vitamin content of the oak tree". Korean J. Medicinal Crop Sci. 6 (1), 6-10). This fruit is about 10 to 30 times more than lemon is known as a report of vitamin C. In addition, beta carotene contains about 8 to 10 times more than carrots. In addition, in the skin of bio-deer fruit, tetracyclic triterpene such as betulinic acid, alphitolic acid, oleanolic acid, maslinic acid, ursolic acid, pomolic acid, tomentic acid, and euscaphic acid, and flavonoids such as quercetin, hyperin, and tiliroside were reported. Kuang, H et al., 5, "Chemical constituents of pericarps of Rosa davurica Pall.a traditional chinese medicine. Chem.Pharm.Bull., 37: 2232-2233) and in the roots Davuricin D1, D2, T1, Tannins such as M1, 1,2,3,6-tetra-O-galloyl-beta-D-Glucose, 1,2,3,4,6-penta-O-galloyl-beta-D-glucose are reported separately. (Yoshida, T et al. 2, Taxifolin apioside and davuricin M1, a hydrolysable tannin from Rosa davurica. Phytochemistry, 28: 2177-2181, Yoshida, T et al. "Hydrolysable tannin oligomers from Rosa davurica. Phytochemistry, 30 (2747-2752)

The present invention is a mixture of water, acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane, lower alcohols having 1 to 4 carbon atoms or mixed with water as extract solvents on leaves, berries, roots, stems, etc. Add 1 ~ 15 times the dry weight of fruit, root or stem bark, extract it by heating at 50 ~ 95 ℃ for 1 ~ 20 hours in the extractor equipped with cooling condenser to prevent evaporation of active ingredient or 1 at 5 ~ 45 ℃. Immerse the active ingredient for 15 days. The obtained extract is filtered through a filter cloth, and then concentrated under reduced pressure to obtain a bio-heat extract of the present invention. In detail, the following Examples. 1 to 3 to obtain a bio-heat extract and obtained the samples used in Examples 4 to 6, Experimental Examples 1 to 3, but the method of producing a bio-heat extract is Example. It is not limited to three.

Example 1

After extracting 1 kg of leaves of fresh heat wood (taken in November) from 50% ethanol for 3 days at 15 ~ 30 ℃, filtered with 300 mesh filter cloth, and dried at 70 ℃ using a rotary vacuum evaporator to obtain 56.5g.

Example. 2

After extracting 1kg of the fruit of the wild oak tree in 50% ethanol for 3 days at 15 ~ 30 ℃, filtered through 300 mesh filter cloth and dried at 70 ℃ by rotary evaporator to obtain 79.4g

Example 3

After extracting the root and stem bark mixture of the bark of the bark tree in 50% ethanol for 3 days at 15-30 ℃, filtered with 300 mesh filter cloth and dried at 70 ℃ by rotary evaporator to obtain 32.3g

Experimental ability to promote collagen synthesis in fibroblasts using the bio-ear extracts (Examples 1 to 3) thus obtained and the wound healing effect when applied to the wound of rats (Experimental Example 2) , Melanin production inhibitory effect (Experimental Example. 3) Tests and Examples. 4 to 6 can be used to produce cosmetics for external application of the skin and using the sample prepared so that the wrinkles of the skin when actually applied to the wrinkled skin of the human It was confirmed that the improvement effect (Experimental Example 4) and the whitening effect (Experimental Example 5) targeting bleeding patients were excellent.

Experimental Example 1 Collagen Production Promoting Effect

Human fibroblasts were placed in a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal bovine serum at 5,000 cells / well, about 70-80%. Incubate until grown. Then, the bio-ear extract was treated for 1 day at concentrations of 0.05% and 0.01%, and then the cell culture was collected. The cultured collagen production was measured using a commercially available collagen protein measuring instrument (Takara Shuzo Co., Ltd. Catalog #: MK101). First, the cell culture medium was placed in a 96-well plate uniformly coated with primary collagen antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours. After 3 hours, the chromophore-bound secondary collagen antibody was added to a 96-well plate and reacted again for 15 minutes. After 15 minutes, a color-causing substance is added, causing color development at room temperature for 15 minutes, and 1M sulfuric acid is added again to stop the reaction (color development). The color of the reaction solution is yellow and the degree of yellow color varies depending on the degree of reaction. The yellow-run 96-well plate was measured at 450 nm using an absorbance meter, and the synthesis degree of collagen was calculated by Equation 1 below. At this time, the reaction absorbance of the prepared cell culture medium of the group not treated with the composition was used as a control.

Formula 1: Collagen Synthesis (%) = (Reaction Absorbance of Cell Culture Solution Extracted / Response Absorbance of Control) * 100

matter % Creation Bio-ear extract of Example 1 0.05% 80.1 Bio-extract extract of Example 1 0.01% 65.7 Bioheat extract of Example 2 0.05% 71.2 Bio-extract extract of Example 2 0.01% 48.1 Bioheat extract of Example 3 0.05% 50.3 Bio-extract extract of Example 3 0.01% 26.4 Control 0

From Table 1 it can be seen that the bio-ear extract has a collagen production promoting effect.

Experimental Example 2 Wound Healing Effects on Rats

A 5 week-old male rat was tested for wound healing using a tensile strength method that reflects the quality and quantity of wound regeneration in the incision. After removing the dorsal hair of the rat, the incision was made with a scalpel and the incision was closed. Example 1 The extracts prepared in 1 to 3 were dissolved in distilled water at 10% and then administered to the incision site for 0.5 days / cm 2 once daily for 6 days. After 6 days, the rats were used for slaughter, the skin of the wound was extracted, and three specimens of width 1cm orthogonal to the incision were made for each subject, and the tear strength was measured by a tension gauge (Rheometer). The measured tensile strength (g / cm) is shown in Table 2 with the strength of the regenerated collagen fibers as an index. Tensile strength of the control group to which only distilled water was applied without adding a sample was expressed as relative strength.

matter Relative Tensile Strength (%) Bio-heat extract of Example 1 0.05% 190 Bio-extract extract of Example 1 0.01% 135 Bioheat extract of Example 2 0.05% 210 Bio-extract extract of Example 2 0.01% 175 Bioheat extract of Example 3 0.05% 160 Bio-extract extract of Example 3 0.01% 121 Control 100

It can be seen from Table 2 that the extract of heat extract has an excellent wound healing effect in rats.

In particular, the extract from the fruit can be seen that the wound healing effect is better.

Experimental Example 3 Inhibition of Melanin Production Using Tyrosinase

In order to determine the whitening effect of the extract of the two extracts according to Example 1, the functional inhibitory experiments were performed on tyrosinase enzyme, and as the comparative example, kojic acid, arbutin, hydroquinone, licorice extract, which are widely used as an active ingredient of the whitening agent, were used.

The test method was put 15 μl of the sample in a microplate (96well), 150 μl of 0.1 M phosphate buffer (pH 6.86), 25 μl of 1.5 mM L-tyrosine solution, and 2,380 units / ml mushroom tyrosinase (Sigma, USA) ( 7 μl of 0.05 M phosphate buffer, pH 6.86) was added thereto, followed by reaction for 10 minutes at 30 ° C., and then the flaw was measured at 490 nm using a microplate. Inhibition rate (%) for tyrosinase was calculated by the following formula 1, it is shown in Table 1 below. In Table 1, the IC50 value is the concentration of the inhibitor reaching 50% enzyme activity inhibition rate.

[Calculation 2]

% Inhibition = [(D-C)-(B -A))] / (D-C) × 100

A: absorbance before reaction of the inhibitor

B: absorbance after the reaction of the inhibitor

C: Absorbance before reaction of the absence of inhibitor

D: absorbance after the reaction without the inhibitor

matter Mushroom Tyrosinase IC50 Bio-ear extract of Example 1 0.025% Biofuel extract of Example 2 0.042% Biothermal extract of Example 3 0.021% Kojic acid 0.037% Arbutin 0.400% Hydroquinone 0.007% Licorice extract 0.014%

As shown in Table 3, it can be seen that the tyrosinase IC50 values of the extracts extracted from the leaves and the extracts extracted from the roots used in the present invention are 0.025% and 0.021%, which have very excellent tyrosinase inhibitory activity.

Example 4 Softening Longevity (Skin) Composition Containing Bio-Heat Extract (Example. 1)

Biothermal ear extract (Example. 1) 0.1%

Glycerin 2%

1,3-butylene glycol 3%

Allantoin 0.1%

DL-panthenol 0.1%

Carboxy vinyl polymer 0.1%

EDTA 0.02%

Ethanol 5%

Polyoxyethylene 60 Cured Castor Oil 0.1%

Triethanolamine 0.1%

Preservatives, Flavors and Pigments

Amount of distilled water

Example 5 Nutrients Containing Bio-Deer Extract

Bio-Heat Extract (Example. 1) 0.5%

Glyceryl Stearate SE 1.5%

Stearyl Alcohol 1.0%

Polysorbate 60 1.2%

Solbitan Stearate 0.4%

10% of liquid paraffin

Vegetable oil 5%

Squalane 2%

Tocopherol acetate 0.5%

Carboxy vinyl polymer 0.14%

Glycerin 3%

1,3-butylene glycol 3.00%

Sodium hyaluronate 0.15%

Triethanolamine 0.14%

Preservatives, Flavors and Pigments

Amount of distilled water

Example 6 Nutritional Cream Containing Bio-Heat Extract

Bio-Heat Extract 0.5%

Lipophilic monostearineglycerin 2.0%

Stearyl Alcohol 2.5%

Stearic acid 1.3%

Beeswax 1.0%

Polysorbate 60 1.5%

Sorbitan stearate 0.5%

Vegetable oil 1.0%

Liquid paraffin 5.0%

Sodium magnesium silicate 3.0%

Glycerin 2.5%

Triethanolamine 1.3%

Sodium Hyaluronate 0.5%

Preservatives, Flavors and Pigments

Amount of distilled water

Experimental Example 4 Measuring the Wrinkle Improvement Effect through Human Experiment

Example. The skin wrinkle improvement effect on the cream prepared in 6 was compared. In other words, the wrinkle improvement effect of the skin is divided into two groups (A, B) 30 women in their 30s to 40s, the cream of Example 6 (A) and the cream without bio-ear extract from Example 6 (B) ) Were given once a day for 1 month.

After one month, the degree of wrinkle improvement, skin elasticity, and moisturizing were investigated using a questionnaire, and the results were averaged and the results are shown in Table 4.

Clinical results after 1 month of use Example 6 (Including Fresh Ear Extract) Example 6 (excluding fresh ear extract) wrinkle improvement(%) 90 45 Skin elasticity improvement (%) 83 37 Moisturizing power improvement (%) 85 42

Experimental Example 5 Whitening Clinical Efficacy Test

Nutritional cream (Sample A) and invention (extractable extract) of Example 6 incorporating the present invention to 60 subjects (30 for each sample) who suffer from pigmentation such as blemishes on the face. A control sample (Sample B) except for the bay was applied to the blemishes of the face twice daily for three months, once each morning and evening.

In evaluating the effect, the degree of improvement of pigmentation after use was determined according to the following criteria.

Excellent effect: Pigmentation is hardly noticeable.

With effect: Pigmentation is very soft.

Slightly effective: Pigmentation is softened.

No effect: No change.

The test results are shown in the table below. Creams containing bio-ear extract, such as 5, clearly show a difference.

Clinical trial of whitening cream containing bio-ear extract Sample..A Sample B Whitening effect Excellent effect Effect slightly

* Criterion for effectiveness

Effective: Over 80% of the subjects show excellent or effective effects

Effective: 50% or more and less than 80% of the subjects show excellent or effective effects

Slightly effective: 30% or more but less than 50%

No effect: less than 30% of subjects demonstrate excellent or effective effects

As described above, the bio-heat extract may provide an effective functional cosmetic composition that prevents the production of melanin pigment, helps regeneration of the wound, and increases collagen production.

Claims (2)

Cosmetic composition containing bio-ear extract The biodeer extract of claim 1 includes flowers, berries, and roots of the biodeer.