KR101985884B1 - Skin external composition comprising flower vinegar extract - Google Patents

Abstract

The present invention relates to an external composition for skin containing the flower extract, more specifically Camellia japonica ) Vinegar Extract and Water Lily ( Nymphaea) Alba ) relates to an external composition for skin containing at least one of vinegar extract, more specifically, containing at least one of camellia vinegar extract and water lily vinegar extract to improve skin wrinkles, improve skin elasticity, reduce pores, reduce sebum, The present invention relates to a skin external preparation composition having excellent acne improving and whitening effects.

Description

Skin external composition comprising flower vinegar extract

The present invention relates to an external composition for skin containing the flower extract, more specifically Camellia japonica ) Vinegar Extract and Water Lily ( Nymphaea) Alba ) relates to an external composition for skin containing at least one of vinegar extract, more specifically, containing at least one of camellia vinegar extract and water lily vinegar extract to improve skin wrinkles, improve skin elasticity, reduce pores, reduce sebum, The present invention relates to a skin external preparation composition having excellent acne improving and whitening effects.

Vinegar is a kind of sour seasoning, and since it is produced through acetic acid fermentation, vinegar has been usefully used as a stored food containing acetic acid. These vinegars are largely classified into brewed vinegar and synthetic vinegar according to the manufacturing method, and brewed vinegar is made by the brewing method. Can be. Synthetic vinegar is manufactured by a chemical process, and includes pure synthetic vinegar and vinegar added to the synthetic vinegar.

Camellia according to the invention japonica ) or water lily ( Nymphaea) There has been no study or report on the excellent effects on the skin of acetic acid fermentation of Alba ) as a vinegar extract.

Korean Patent Publication No. 10-2010-0057460

In this regard, the inventors of Camellia japonica ) Vinegar Extract and Water Lily ( Nymphaea) Alba ) The vinegar extract was found to exhibit a remarkably excellent effect in enhancing skin elasticity, improving skin wrinkles, shrinking pores, sebum control and whitening effect, and completed the present invention.

Accordingly, it is an object of the present invention to provide a skin external preparation composition containing at least one of camellia vinegar extract and water lily vinegar extract, which exhibits skin elasticity enhancement, skin wrinkle improvement, pore reduction, sebum control and whitening effect.

In order to achieve the above object, the present invention provides an external composition for skin containing at least one of camellia vinegar extract and water lily vinegar extract.

The present invention also provides a skin external composition for improving skin wrinkles containing at least one of camellia vinegar extract and water lily vinegar extract.

In addition, the present invention provides a skin external composition for enhancing skin elasticity containing at least one of camellia vinegar extract and water lily vinegar extract.

In addition, the present invention provides a skin external composition for reducing pores of at least one of camellia vinegar extract and water lily vinegar extract.

The present invention also provides a skin external composition for sebum reduction containing at least one of camellia vinegar extract and water lily vinegar extract.

The present invention also provides a skin external preparation composition for acne improvement containing at least one of camellia vinegar extract and water lily vinegar extract.

The present invention also provides a skin whitening composition for skin whitening containing at least one of camellia vinegar extract and water lily vinegar extract.

The composition according to the present invention may contain at least one of camellia vinegar extract and water lily vinegar extract to provide skin wrinkle improvement, skin elasticity enhancement, pore reduction, sebum reduction, acne improvement and skin whitening effect, camellia vinegar extract and In the case of containing a mixed extract of water lily vinegar extract shows a remarkable effect through the synergistic effect. In particular, compared to the general camellia extract, general water lily extract, or a combination thereof, it is possible to provide an external composition for skin, which shows remarkably excellent effects in improving skin wrinkles, enhancing skin elasticity, reducing pores, reducing sebum, improving acne, and skin whitening. Can be.

The composition for external application for skin according to the present invention is Camellia japonica ) Vinegar Extract and Water Lily ( Nymphaea) Alba ) Contains at least one kind of vinegar extract as an active ingredient.

Camellia used in the present invention japonica ) is an evergreen tree of the tea tree family, and is distributed mainly in East Asia including Korea. Oil is used to make camellia oil from seeds and wood is used for furniture and handiwork. In the present invention, the camellia may use any part of the plant such as roots, stems, leaves, and flowers, and may be used as an entire plant (outpost).

Water lily ( Nymphaea) used in the present invention Alba ) is a water lily and aquatic plant, also known as white lily, contains alkaloids and is used as a sedative. Roots, stems and flowers have been used as medicine in traditional medicine. In the present invention, the water lily can be used for all parts of the plant, such as roots, stems, leaves, flowers, can also be used as a whole plant (outpost).

The camellia vinegar extract according to the present invention means obtained by acetic acid fermentation of the camellia. In addition, the camellia vinegar extract may be prepared through a saccharification process, alcohol fermentation process and acetic acid fermentation process.

In one embodiment of the present invention, the camellia vinegar extract may be contained in an amount of 0.001% by weight or more based on the total weight of the composition, preferably in an amount of 10.0% by weight or more. In another embodiment of the present invention, the camellia vinegar extract may be contained in an amount of 90.0% by weight or less based on the total weight of the composition, preferably in an amount of 70.0% by weight or less. In another embodiment of the present invention, the camellia vinegar extract may be contained in an amount of 0.001% by weight or more and 90.0% by weight or less with respect to the total weight of the composition, more preferably 10% by weight or more and 70.0% by weight or less It may contain. If it is used at less than 0.001% by weight, its efficacy may not be sufficiently exhibited. If it is used at more than 90.0% by weight, the pH is low, which may cause skin irritation.

The water lily vinegar extract according to the present invention means obtained by acetic acid fermentation of the water lily. In addition, the water lily vinegar extract may be prepared through a saccharification process, alcohol fermentation process and acetic acid fermentation process.

In one embodiment of the present invention, the water lily vinegar extract may be contained in an amount of 0.001% by weight or more based on the total weight of the composition, preferably in an amount of 10.0% by weight or more. In another embodiment of the present invention, the water lily vinegar extract may be contained in an amount of 90.0% by weight or less based on the total weight of the composition, preferably in an amount of 70.0% by weight or less. In another embodiment of the present invention, the water lily vinegar extract may be contained in an amount of 0.001% by weight or more and 90.0% by weight or less with respect to the total weight of the composition, more preferably 10% by weight or more and 70.0% by weight or less It may contain. If it is used at less than 0.001% by weight, its efficacy may not be sufficiently exhibited. If it is used at more than 90.0% by weight, the pH is low, which may cause skin irritation.

In an embodiment of the present invention, when the camellia vinegar extract and the water lily vinegar extract are contained, the camellia vinegar extract and the water lily vinegar extract may be contained in a ratio of 2: 8 to 8: 2 by weight, preferably May be contained in a ratio of 4: 6 to 6: 4. If the ratio is outside the ratio of 2: 8 to 8: 2, it is concentrated only on one efficacy, and the synergy of the efficacy is not sufficiently exerted. In addition, when simultaneously containing the camellia vinegar extract and water lily vinegar extract as an active ingredient, the total content of the active ingredient may contain 0.001% by weight or more and 90.0% by weight or less, preferably 10% by weight or more and 90.0% by weight or less. It may contain. If it is used at less than 0.001% by weight, its efficacy may not be sufficiently exhibited. If it is used at more than 90.0% by weight, the pH is low, which may cause skin irritation.

The composition according to the present invention can be used as a skin external preparation composition for enhancing skin elasticity, and can be used as a skin external preparation composition for improving skin wrinkles. It is excellent in promoting the collagen synthesis of the skin, it can improve the skin elasticity to improve the skin wrinkles and improve the skin sag can provide a lifting effect.

The composition according to the present invention can be used as a skin external preparation composition for pore reduction and sebum control, which is excellent in the effect of suppressing excessive secretion of sebum when applied to the skin and reducing pore size.

The composition according to the present invention can be used as an external preparation composition for improving acne, has an excellent antibacterial effect against acne causing bacteria, and is excellent in inhibiting skin troubles.

The composition according to the present invention can be used as a skin external preparation composition for skin whitening, which can provide an excellent whitening effect by inhibiting tyrosinase activity and inhibiting the production of melanin.

The composition according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) and non-obtained by dispersing an oil phase in solution, gels, solids, pasty anhydrous products, aqueous phases. It may be provided in the form of an ionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions can be prepared according to conventional methods in the art.

In addition, the composition according to the present invention is a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or nonionic. Such as type emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics. It may contain adjuvants conventionally used in the cosmetic or dermatology field. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

Hereinafter, the configuration and effects of the present invention will be described in more detail with examples of test and formulation. However, these test examples and formulation examples are provided only for the purpose of illustration to help the understanding of the present invention is not limited to the scope and scope of the present invention by the following examples.

Preparation Example 1 Preparation of Camellia Vinegar Extract

1. Base liquor manufacturing process (glycosylation process)

After washing 20 kg of brown rice, immersed in 30 kg of bottled water (160m rock water) for 5 hours, drain and prepare water, and prepare the wild camellia flower by extracting, cutting, washing, and draining water. After steaming for 25 to 35 minutes at a temperature of -110 degreeC, it cools to 60 degreeC. 2 kg of malt powder is added to the cooled brown rice and saccharified for 12 hours. 6 kg of wheat malt was added thereto to saccharify the yeast by activating the yeast bacterium at a temperature of 30 ° C. for 48 hours, and then put the obtained rice into a jar and add 40 kg of bottled water (160m rock water) to obtain air from the outside at a temperature of 25 ° C. for 72 hours. The base liquor is prepared by blocking and keeping the carbon dioxide out.

2. Alcohol Fermentation Process

After washing 13kg of brown rice, immersed in 20kg of water for one hour, drained water and 1kg of the remaining brown rice and camellia flower at a temperature of 90 ~ 110 ℃ 25 to 35 minutes to prepare a rice paddy. Put the prepared rice and camellia 10kg in the base liquor prepared in 1. ferment alcohol for 10-15 days at 25 ℃.

3. Acetic Acid Fermentation Process

After diluting 1 kg of water into the fermentation product obtained in 2. and sealing the inlet with a cloth to allow air to enter the jar at 27 ° C. for 2 months, supplying oxygen while stirring occasionally to ferment the primary (aerobic) acetic acid . After measuring the acidity of the obtained fermented product (PH-230SD PH meter) to confirm that the pH is 4 or more, only the supernatant is separated and aged in a shaded cool place of 5 ~ 20 ℃ second. After that, the aged liquid was filtered to obtain a camellia vinegar extract. The resulting camellia vinegar extract was sterilized, bottled and stored in a cool dark place, which was used for the following test.

Preparation Example 2 Preparation of Water Lily Vinegar Extract

1. Base liquor manufacturing process (glycosylation process)

After washing 20 kg of brown rice, immersed in 30 kg of bottled water (160m rock water) for 5 hours, drain and prepare water, and prepare the wild water lily by extracting, cutting, washing and draining water. The mixture is then steamed at a temperature of 90 to 110 ° C. for 25 to 35 minutes and then cooled to 60 ° C. 2 kg of malt powder is added to the cooled brown rice and saccharified for 12 hours. 6 kg of wheat malt was added thereto to saccharify the yeast by activating the yeast bacterium at a temperature of 30 ° C. for 48 hours, and then put the obtained rice into a jar and add 40 kg of bottled water (160m rock water) to obtain air from the outside at a temperature of 25 ° C. for 72 hours. The base liquor is prepared by blocking and keeping the carbon dioxide out.

2. Alcohol Fermentation Process

After washing 13kg of brown rice, immersed in 20kg of water for one hour, drained water and steamed 1kg of the remaining brown rice and water hydration at a temperature of 90 ~ 110 ℃ 25 to 35 minutes to prepare a rice paddy. Put the prepared rice and water lily 10kg in the base liquor prepared in 1. ferment alcohol for 10-15 days at 25 ℃.

3. Acetic Acid Fermentation Process

After diluting 1 kg of water into the fermentation product obtained in 2. and sealing the inlet with a cloth to allow air to enter the jar at 27 ° C. for 2 months, supplying oxygen while stirring occasionally to ferment the primary (aerobic) acetic acid . After measuring the acidity of the obtained fermented product (PH-230SD PH meter) to confirm that the pH is 4 or more, only the supernatant is separated and aged in a shaded cool place of 5 ~ 20 ℃ second. Thereafter, the matured liquid was filtered to obtain a water vinegar extract. The obtained water vinegar extract was sterilized, bottled and stored in a cool dark place, which was provided for the following test.

Comparative Preparation Example 1 Preparation of Camellia Extract

Camellia extract of the present invention was mixed with 10 g of butylene glycol, 71 g of purified water to 19 g of camellia flowers and extracted by heating for 1 hour. Thereafter, the mixture was centrifuged at 8000 rpm and 15 ° C. for 30 minutes, and then filtered sequentially with a 3.00 μm, 1.2 μm, 0.7 μm, and 0.45 μm filter. The extracted stock solution was suspended in an auto mixer to contain 10% butylene glycol for 1 hour at 800 rpm.

 Comparative Preparation Example 2 Preparation of Water Lily Extract

The water lily extract of the present invention was mixed with 10 g of butylene glycol and 71 g of purified water to 19 g of water lily, and extracted by heating for 1 hour. Thereafter, the mixture was centrifuged at 8000 rpm and 15 ° C. for 30 minutes, and then filtered sequentially with a 3.00 μm, 1.2 μm, 0.7 μm, and 0.45 μm filter. The extracted stock solution was suspended in an auto mixer to contain 10% butylene glycol for 1 hour at 800 rpm.

[Examples 1-3 and Comparative Examples 1-4]

Using Examples 1 and 2 and Comparative Preparation Examples 1 and 2, Examples 1 to 3 and Comparative Examples 1 to 4 were prepared according to the compositions of Table 1 below (unit: wt%).

Raw material name Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Water component Camellia vinegar extract (manufacturing example 1) 78 - 39 - - - - Water lily vinegar extract (manufacturing example 2) - 78 39 - - - - Camellia extract (Comparative Preparation Example 1) - - - - 78 - 39 Water lily extract (Comparative Preparation Example 2) - - - - - 78 39 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 Disodium ID 0.02 0.02 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Xanthan Gum 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Methylgluses-20 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceres-26 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Ammonium Acryloyl Dimethyl Taurate / V-Picopolymer 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Ethanol Phase Component ethanol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Hydrogenated Castor Oil 0.3 0.3 0.3 0.3 0.3 0.3 0.3 incense 0.1 0.1 0.1 0.1 0.1 0.1 0.1 antiseptic 0.3 0.3 0.3 0.3 0.3 0.3 0.3

<Production method>

(1) The aqueous phase components (1-9) were uniformly mixed at room temperature.

(2) The ethanol phase components 10-13 were heated to 50-55 ° C. to uniformly dissolve and mix.

(3) The said (1) was thrown into the said (2) under stirring, and it solubilized uniformly.

Test Example 1 Skin Elasticity Improvement Effect

In order to confirm the skin elasticity improving effect according to the present invention was tested as follows using Examples 1 to 3 and Comparative Examples 1 to 4. Each of 35 women in their 30s and 40s divided into 5 groups of 5 people each of Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 twice daily (morning, Evening) after applying to the face for 4 weeks, by measuring the skin elasticity improvement effect using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany), the results are shown in Table 2 below. The results in Table 2 refer to the properties of skin viscoelasticity of the skin elasticity tester.

Test substance Skin elasticity effect Example 1 0.66 ± 0.10 Example 2 0.70 ± 0.10 Example 3 0.75 ± 0.10 Comparative Example 1 0.35 ± 0.09 Comparative Example 2 0.46 ± 0.09 Comparative Example 3 0.44 ± 0.09 Comparative Example 4 0.48 ± 0.09

In the results of Table 2, in the case of Comparative Examples 2 to 4 containing a general camellia extract, a general water lily extract or a combination thereof showed a skin elasticity enhancing effect of about 25 to 37% compared to Comparative Example 1 containing no extract . However, when Examples 1 to 3 containing Camellia vinegar extract, water lily vinegar extract, or a combination thereof were used, it showed that the skin elasticity enhancing effect was about 188 to 215% compared to Comparative Example 1, showing a remarkably excellent effect. In particular, when the camellia vinegar extract and the water lily vinegar extract at the same time through the synergistic effect it can be seen that the skin elasticity enhancing effect is more remarkably excellent.

Test Example 2 Collagen Synthesis Promoting Effect

After culturing human fibroblasts in a 24-well plate incubator, the mixed extract of Camellia vinegar extract, water lily vinegar extract, camellia vinegar extract and water lily vinegar extract of Preparation Examples 1 to 2 at the concentrations of Table 3, Comparative Preparation Example A medium containing 1 to 2 general camellia extracts, general water lily extracts, and mixed extracts of general camellia and water lily extracts was replaced. On the third day of culture, 0.5 ml of DMEM medium containing 10% fetal calf serum was added to each well, followed by 10 μCi of L [2,3,4,5- ³ H] -proline. After 24 hours, the media and cells in each well are scraped and washed in 5% Trichloroacetic acid (TCA) solution, and then dispensed into two test tubes and one type I cola in one test tube. 1 unit / μl of genase (type I collagenase) was added thereto, and incubated at 37 ° C. for 90 minutes, and other test tubes were stored at 4 ° C. Thereafter, 0.05 ml of 50% trichloroacetic acid was added to all test tubes and allowed to stand at 4 ° C. for 20 minutes, followed by centrifugation at 12,000 rpm for 10 minutes, respectively. CPM (Count Per Minute) value was measured, and collagen biosynthesis ratio (RCB; Relative Collagen Biosynthesis) was measured for the control group and the experimental group according to Equation 1 below, and the results are shown in Table 3 below. .

Camellia
Vinegar Extract
(Production Example 1) training
Vinegar Extract
(Production Example 2) Camellia extract
(Comparative Manufacturing Example 1) Water lily extract
(Comparative Production Example 2) Collagen Biosynthesis Rate (RCB) (%) Control
(Untreated group) - - - - 100 Example 4 10 - - - 148 Example 5 - 10 - - 145 Example 6 5 5 - - 152 Comparative Example 5 - - 10 - 125 Comparative Example 6 - - - 10 122 Comparative Example 7 - 5 5 126

In the results of Table 3, in the case of Comparative Examples 5-7 containing the general camellia extract, the general water lily extract, or a combination thereof, the collagen biosynthesis ratio was about 22-26% compared to the control group without the extract. However, when Examples 4 to 6 containing Camellia vinegar extract, water lily vinegar extract, or a combination thereof were used, the collagen biosynthesis ratio was about 45-52% compared to the control group, indicating a remarkably excellent effect. In particular, it can be seen that the collagen biosynthesis ratio is significantly more excellent through the synergistic effect when the camellia vinegar extract and the water lily vinegar extract at the same time.

Test Example 3 Sensory Evaluation of Lifting Efficacy

After dividing 35 women aged 25-35 who are suffering from skin sagging into 7 sets of 5 people each, the skin lotion of Examples 1 to 3 and Comparative Examples 1 to 4 were used in the same manner as general lotion for 4 weeks. One week after the start of use, the skin lifting efficacy of the user was evaluated through a questionnaire according to the following evaluation criteria. The results are shown in Table 4 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: A slight improvement.

±: No improvement but no deterioration.

-: No improvement, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 Weeks Example 1 ++ ++ +++ +++ Example 2 ++ ++ ++ +++ Example 3 ++ +++ +++ +++ Comparative Example 1 ± ± + + Comparative Example 2 + + ++ ++ Comparative Example 3 + + + ++ Comparative Example 4 + + ++ ++

In the results of Table 4, compared with Comparative Example 1, a general camellia extract, a general water lily extract, a mixed extract thereof containing no extract at all, Camellia vinegar extract, water lily vinegar according to the present invention Examples 1-3 containing extracts and their mixed extracts exhibited rapid and excellent lifting efficacy.

Test Example 4 Pore Size Reduction Efficacy

Each of 45 women over 30 years old with pore troubles are divided into three groups of 15 people each, and Examples 1 to 3 are applied daily to the place where the pores stand out for 2 weeks, and then skin touches are performed before and after the test. (Manufacturer: True System) The pore size was measured by the pore analysis program. The average pore size was divided into 3 grades by the pore analysis program, and the number of each group was shown in Table 5 below.

sample Average pore size
(pixel) Pore size
Large group Pore size
Middle group (persons) Pore size
Small group Example
One Before the test 195.3 6 5 4 1 week after the exam 185.0 5 6 4 2 weeks after the test 162.3 4 5 6 Example
2 Before the test 189.1 7 5 3 1 week after the exam 180.0 5 7 3 2 weeks after the test 155.3 4 5 6 Example
3 Before the test 197.5 6 4 5 1 week after the exam 181.6 5 4 6 2 weeks after the test 145.6 3 5 7

In the results of Table 5, it can be seen that the composition according to the present invention can effectively reduce the size of the pores.

Test Example 5 Sensory Evaluation of Pore Reduction Effect

Seventy 25-35 year old women who suffered from large pores were divided into seven groups of ten people each to use the lotion of Examples 1 to 3 and Comparative Examples 1 to 4 for the same use method as general lotion for 4 weeks. According to the opinion of the skin improvement to be judged according to the following evaluation criteria are shown in Table 6 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: A slight improvement.

±: No improvement but no deterioration.

-: No improvement, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 Weeks Example 1 ++ ++ +++ +++ Example 2 ++ +++ +++ +++ Example 3 ++ +++ +++ +++ Comparative Example 1 + + + ++ Comparative Example 2 + ++ ++ ++ Comparative Example 3 + ++ ++ ++ Comparative Example 4 + ++ ++ +++

In the results of Table 6, compared with Comparative Example 1, a general camellia extract, a general water lily extract, a mixed extract thereof containing no extract at all, Camellia vinegar extract, water lily vinegar according to the present invention Examples 1-3 containing the extracts and mixed extracts thereof showed a rapid and excellent pore reduction effect.

[Test Example 6] Confirmation of sebum reduction effect

Thirty subjects in their 20s and 30s, who felt that there was a lot of sebum secretion, were divided into three groups of 10 people each, and the above Examples 1 to 3 were applied daily to the place where the sebum of the face was large for 4 weeks. Before the start of the test and after 2 weeks of testing, after 4 weeks of testing, sebum was measured using a skin oil analyzer (Sebumeter SM810), and the results are shown in Tables 7 to 9 below.

sample Example 1 Subject Before test (㎍ / ㎠h) 2 weeks after the test (㎍ / ㎠h) 4 weeks after the test (㎍ / ㎠h) One 90 85 79 2 86 82 80 3 102 96 85 4 103 82 76 5 94 85 79 6 74 67 60 7 68 53 44 8 65 51 50 9 86 82 74 10 59 56 47

sample Example 2 Subject Before test (㎍ / ㎠h) 2 weeks after the test (㎍ / ㎠h) 4 weeks after the test (㎍ / ㎠h) One 95 81 75 2 92 85 78 3 74 70 65 4 52 45 40 5 94 86 82 6 74 68 60 7 106 99 82 8 65 55 50 9 68 56 43 10 103 95 74

sample Example 3 Subject Before test (㎍ / ㎠h) 2 weeks after the test (㎍ / ㎠h) 4 weeks after the test (㎍ / ㎠h) One 65 60 45 2 58 50 43 3 101 88 65 4 85 80 71 5 77 55 50 6 66 60 44 7 100 85 70 8 88 85 70 9 106 100 91 10 55 50 40

In the results of Tables 7 to 9, it can be confirmed that the composition according to the present invention can effectively inhibit sebum.

Test Example 7 Sensory Evaluation of Acne Improvement Effect

Among 19-26 year old women, 35 persons with acne were divided into 5 groups of 5 people each, and the skin lotion of Examples 1 to 3 and Comparative Examples 1 to 4 were used in the same manner as general lotion for 4 weeks. According to the opinion, the degree of skin improvement was determined according to the following evaluation criteria. The results are shown in Table 10 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: A slight improvement.

±: No improvement but no deterioration.

-: No improvement, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 Weeks Example 1 ++ +++ +++ +++ Example 2 ++ ++ +++ +++ Example 3 +++ +++ +++ +++ Comparative Example 1 + + + ++ Comparative Example 2 + ++ ++ ++ Comparative Example 3 + ++ ++ ++ Comparative Example 4 + ++ ++ +++

In the results of Table 10, compared with Comparative Example 1, a general camellia extract, a general water lily extract, a mixed extract thereof containing no extract at all, Camellia vinegar extract, water lily vinegar according to the present invention Examples 1 to 3 containing the extracts and mixed extracts thereof showed a rapid and excellent acne skin improvement effect.

Test Example 8 Whitening Effect Test on Human Skin

Five sets of 35 women in their 30s and 40s, each with 5 pairs of opaque tape with 6 holes of 1.5cm in diameter, were placed on the upper forearm of the subject, and then 1.5 ~ 2 times the minimum erythema dose of each subject. UVB is irradiated to a degree of blackening of the skin, and the pores of Examples 1-3 and Comparative Examples 1 to 4 are applied to the respective pores twice daily in the morning and evening, and 50 days later using a color difference meter. The contrast of the skin was measured.

The determination of the effect was determined by measuring the degree of black and white of the skin using a color difference meter (Minolta CR2002). L, a, and b colorimeters are used to display the color. In the present invention, the "L" value representing the contrast of the skin was obtained and analyzed (the unburned Korean skin color generally shows a value of 50 to 70). The L value was calibrated with a standard whiteboard, and the measurement was measured evenly by repeating the measurement five times or more at one site.

When the test substance is effective, the L value is gradually increased, and the comparison between the test substances is calculated according to the following equation (2) of the difference in skin color (ΔL) between the start point and the end point of the application. The results are shown in Table 11 below.

Test substance Whitening Effect (△ L) Example 1 4.22 Example 2 4.02 Example 3 5.04 Comparative Example 1 0.88 Comparative Example 2 1.92 Comparative Example 3 2.05 Comparative Example 4 2.56

In the results of Table 11, when using Examples 1 to 3 containing camellia vinegar extract, water lily vinegar extract or a combination thereof compared to Comparative Examples 2 to 4 containing a general camellia extract, general water lily extract or a combination thereof It showed a remarkable whitening effect of 1.5 ~ 3 times. In particular, when the camellia vinegar extract and water lily vinegar extract at the same time through the synergistic effect it can be seen that the whitening effect is significantly more excellent.

Claims (15)

A skin external preparation composition for improving acne containing at least one of Camellia japonica vinegar extract and Nymphaea Alba vinegar extract as an active ingredient. A skin external preparation composition for skin whitening containing at least one of camellia vinegar extract and water lily vinegar extract as an active ingredient. A skin external preparation composition for improving skin wrinkles containing at least one of camellia vinegar extract and water lily vinegar extract as an active ingredient. A skin external preparation composition for enhancing skin elasticity containing at least one of camellia vinegar extract and water lily vinegar extract as an active ingredient. A skin external preparation composition for reducing pores containing at least one of a camellia vinegar extract and a water lily vinegar extract as an active ingredient. Skin external composition for sebum reduction containing at least one of camellia vinegar extract and water lily vinegar extract as an active ingredient. The method according to any one of claims 1 to 6,
The active ingredient is an external composition for skin, characterized in that it contains 0.001 to 90% by weight based on the total weight of the composition. delete The skin external composition according to any one of claims 1 to 6, wherein the camellia vinegar extract and the water lily vinegar extract are contained in a weight ratio of 2: 8 to 8: 2. A cosmetic method for improving skin wrinkles by using the composition according to claim 3 as a cosmetic. A cosmetic method for enhancing skin elasticity by using the composition according to claim 4 as a cosmetic. A cosmetic method for shrinking skin pores by using the composition according to claim 5 as a cosmetic. A cosmetic method for reducing sebum of the skin by using the composition according to claim 6 as a cosmetic. A cosmetic method for improving acne by using the composition according to claim 1 as a cosmetic. A cosmetic method for promoting skin whitening by using the composition according to claim 2 as a cosmetic.