KR102108203B1 - Skin external composition comprising Lonicera japonica vinegar extract - Google Patents

Abstract

The present invention relates to a composition for external application for skin containing an extract of vinegar of Indongcho, and more specifically, to a composition for external application for skin having an effect of skin elasticity, pore shrinking, sebum reduction and acne improvement by containing the extract of Indongcho vinegar.

Description

Skin external composition comprising Lonicera japonica vinegar extract

The present invention relates to a composition for external application for skin containing an extract of vinegar of Indongcho, and more specifically, to a composition for external application for skin having an effect of skin elasticity, pore shrinking, sebum reduction and acne improvement by containing the extract of Indongcho vinegar.

Vinegar is a kind of seasoning that has a sour taste, and since it is produced through acetic acid fermentation, it has been usefully used as a stored food containing acetic acid. These vinegars are largely classified into brewed vinegar and synthetic vinegar according to the manufacturing method, and brewed vinegar refers to those made by brewing method. Can be. Synthetic vinegar is produced by a chemical process, and includes synthetic vinegar added to pure synthetic vinegar and brewed vinegar.

Indongcho is also known as the gold-silver coin. It is a perennial vine plant belonging to the Indongdae family and is mainly distributed in East Asia including Korea. Because of its antipyretic, detoxifying and sweating properties, stems have been used to treat colds, and have been used as treatments for infectious hepatitis and boils.

As in the present invention, there has been no research or report on the excellent effect on the skin when fermented indongcho is acetic acid and used as a vinegar extract.

Korean Patent Publication No. 10-2004-21589 (Publication date: 2004.03.10)

Accordingly, the present inventors discovered that the extract of Indongcho vinegar exhibits a remarkably excellent effect on skin elasticity, pore reduction, sebum reduction and acne improvement effect, and completed the present invention.

Accordingly, an object of the present invention is to provide a composition for external application for skin that contains an extract of vinegar of Indongcho, showing skin elasticity, pore shrinking, sebum control and acne improvement effects.

In order to achieve the above object, the present invention provides a composition for external application for skin for acne improvement containing an extract of vinegar of Indongcho.

The composition according to the present invention can provide skin elasticity, pore reduction, sebum reduction and acne improvement effects by containing the extract of vinegar from Indongcho, and in particular, the effect of skin elasticity, pore reduction, sebum reduction and acne improvement even when compared to the normal Indongcho extract It can provide a composition for external application for skin exhibiting remarkably excellent efficacy.

The composition for external application for skin according to the present invention contains inactive vinegar extract ( Lonicera japonica ; Honeysuckle) as an active ingredient.

Indongcho used in the present invention is also called a gold and silver coin, and is a perennial vine plant belonging to the family A, which is mainly distributed in East Asia including Korea. Because of its antipyretic, detoxifying and sweating properties, stems have been used to treat colds, and have been used as treatments for infectious hepatitis and boils.

In the present invention, the Indongcho ( Lonicera japonica ) can use all parts of the plant, such as roots, stems, leaves, and flowers, and can also be used as whole plants (outposts). Indongcho vinegar extract according to the present invention means obtained by fermenting the indongcho vinegar. In addition, the vinegar extract of Indongcho can be prepared through a saccharification process, an alcohol fermentation process, and an acetic acid fermentation process.

In one embodiment of the present invention, the vinegar extract of Indongcho may be contained in an amount of 0.001% by weight or more based on the total weight of the composition, and preferably in an amount of 10.0% by weight or more. In another embodiment of the present invention, the vinegar extract of Indongcho may be contained in an amount of 90.0% by weight or less based on the total weight of the composition, and preferably in an amount of 70.0% by weight. In another embodiment of the present invention, the vinegar extract of Indongcho may be contained in an amount of 0.001% by weight or more and 90.0% by weight or less based on the total weight of the composition, more preferably 10.0% by weight or more and 70.0% by weight or less It can contain. When used in less than 0.001% by weight, its efficacy cannot be sufficiently exhibited, and when it is used in excess of 90.0% by weight, the pH is low, which may cause irritation.

The composition according to the present invention can be used as a composition for external application for skin for enhancing skin elasticity. This has an excellent effect of promoting the collagen synthesis of the skin, and can improve the skin elasticity to improve the sagging of the skin to provide a lifting effect.

The composition according to the present invention can be used as a composition for external application for skin for pore reduction and sebum control, which suppresses sebum secreted excessively when applied to the skin and has an excellent effect of reducing pore size.

The composition according to the present invention can be used as a composition for external application for skin for improving acne, has excellent antibacterial effect against acne-causing bacteria, and has excellent skin trouble suppressing effect.

The composition according to the invention may be formulated containing cosmetic or dermatologically acceptable media or bases. These are all formulations suitable for topical application, for example solutions, gels, solids, dough anhydrous products, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) and ratios obtained by dispersing an oil phase in an aqueous phase. It can be provided in the form of an ionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions can be prepared according to conventional methods in the art.

In addition, the composition according to the present invention is a fat substance, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizing agent, a foaming agent, a fragrance, a surfactant, water, ionic or nonionic Type emulsifiers, fillers, metal ion blockers, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics. It may contain adjuvants commonly used in cosmetic or dermatological fields. The adjuvant is introduced in an amount commonly used in the cosmetic or dermatological field.

In addition, the composition of the present invention may contain a skin absorption promoting substance in order to increase the skin improving effect.

Hereinafter, the composition and effects of the present invention will be described in more detail with reference to test examples and formulation examples. However, these test examples and formulation examples are provided for illustrative purposes only to aid understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Production Example 1] Preparation of Indongcho vinegar extract

1. Base liquor manufacturing process (glycation process)

After washing 20 kg of brown rice, immersed in 30 kg of mineral water (160 m bedrock water) for 5 hours, the water is decanted and prepared, and wild injured grass is collected, cut, washed, and drained to prepare water. After increasing the temperature for 25 to 35 minutes at a temperature of ~ 110 ° C, it is cooled to 60 ° C. Add 2 kg of malt flour to the cooled brown rice and saccharify for 12 hours. Here, 6 kg of wheat malt was added, saccharified at a temperature of 30 ° C. for 48 hours to activate the yeast, and the obtained rice was placed in a jar and 40 kg of bottled water (160 m rock water) was added to air from the outside at a temperature of 25 ° C. for 72 hours. Blocks and discharges only carbon dioxide to maintain the base liquor.

2. Alcohol fermentation process

After washing 13 kg of brown rice, immersed in 20 kg of water for one hour, decanted water, and the remaining brown rice and 1 kg of Indongcho outpost are steamed at a temperature of 90 to 110 ° C for 25 to 35 minutes to prepare rice paddy. 9 kg of the prepared rice pad and indongcho outpost are added to the base liquor prepared in 1. and alcohol fermentation is performed at 25 ° C. for 10-15 days.

3. Acetic acid fermentation process

After diluting 1 kg of water into the fermented product obtained in the step 2, the entrance is sealed with a cloth to allow air to enter the jar for 2 months at 27 ° C., and oxygen is supplied while stirring occasionally to give primary (aerobic) acetic acid fermentation. . After measuring the acidity of the obtained fermentation product (PH-230SD PH meter) to confirm that the pH is 4 or higher, only the supernatant is separated, and then secondary aged in a shaded and cool place at 5 ~ 20 ℃. After that, the aged liquid was filtered to obtain an extract of Indongcho vinegar. The obtained vinegar extract of Indongcho was sterilized and bottled and stored in a cold dark place, which was provided in the following test.

[Comparative Production Example 1] Preparation of Indongcho extract

Indongcho extract of the present invention was mixed with 10 g of butylene glycol and 71 g of purified water to 19 g of Indongcho outpost, followed by heating extraction for 1 hour. Thereafter, centrifugation was performed at 8000 rpm at 15 ° C for 30 minutes, followed by sequentially filtering with 3.00 μm, 1.2 μm, 0.7 μm, and 0.45 μm filters. The extracted stock solution was suspended in an auto mixer at 800 rpm for 1 hour to contain 10% butylene glycol to obtain an extract of Indongcho.

[Example 1 and Comparative Examples 1 to 2]

Example 1 and Comparative Examples 1 to 2 were prepared according to the composition of Table 1 using Preparation Example 1 and Comparative Preparation Example 1 (unit: wt%).

Raw material name Example 1 Comparative Example 1 Comparative Example 2 One Indongcho vinegar extract (Production Example 1) 78 - - 2 Indongcho Extract (Comparative Production Example 1) - - 78 3 Purified water To 100 To 100 To 100 4 Disodium LED 0.02 0.02 0.02 5 glycerin 5.0 5.0 5.0 6 Xanthan gum 0.15 0.15 0.15 7 Methylgluceth-20 2.0 2.0 2.0 8 Glyceres-26 2.0 2.0 2.0 9 Ammonium acryloyl dimethyl taurate / VP copolymer 0.3 0.3 0.3 10 ethanol 3.0 3.0 3.0 11 Hydrogenated castor oil 0.3 0.3 0.3 12 incense 0.1 0.1 0.1 13 antiseptic 0.3 0.3 0.3

<Manufacturing method>

(1) The aqueous phase components (1-9) were uniformly mixed at room temperature.

(2) Ethanol components (10-13) were uniformly dissolved and mixed by heating to 50-55 ° C.

(3) The above (2) was added to (2) under stirring to solubilize uniformly.

[Test Example 1] Skin elasticity improvement effect

In order to confirm the effect of improving skin elasticity according to the present invention, it was tested as follows using Example 1 and Comparative Examples 1-2. After dividing 80 women in their 30s and 40s into 3 sets of 20 each, apply Example 1, Comparative Example 1, and Comparative Example 2 twice daily (morning and evening) to the face for 4 weeks, and then measure the skin elasticity (Cutometer). SEM 575, C + K Electronic Co., Germany) was used to measure the effect of improving skin elasticity, and the results are shown in Table 2 below. The results in Table 2 indicate the properties of the skin viscoelasticity of the skin elasticity meter.

Test substance Skin elasticity effect Example 1 0.66 ± 0.10 Comparative Example 1 0.30 ± 0.09 Comparative Example 2 0.42 ± 0.09

In the results of Table 2, in the case of Comparative Example 2 containing the common extract of Indongcho, it showed an effect of enhancing skin elasticity of about 140% compared to Comparative Example 1. However, when Example 1 containing the extract of vinegar of Indongcho was used, it was found that the effect of promoting skin elasticity was improved by about 220% compared to Comparative Example 1, indicating a remarkably excellent effect.

[Test Example 2] Effect of promoting collagen synthesis

After culturing human fibroblasts in a 24-well plate incubator, they were replaced with a medium containing the extract of Indongcho vinegar of Preparation Example 1 and the extract of normal Indongcho of Comparative Preparation Example 1 at the concentrations in Table 3 below. On the third day of culture, 0.5 ml of each DMEM medium containing 10% fetal calf serum was added, and then 10 [mu] Ci of L [2,3,4,5- ³ H] -proline was added. After 24 hours, the media and cells in each well were scraped and washed in 5% trichloroacetic acid (TCA) solution, then dispensed into 2 tubes and type I coke in 1 tube 1 unit / µl of Genase (type I collagenase) was added, incubated at 37 ° C for 90 minutes, and other test tubes were stored at 4 ° C. Subsequently, 0.05 ml of 50% trichloroacetic acid was added to all test tubes, and allowed to stand at 4 ° C. for 20 minutes, followed by centrifugation at 12,000 rpm for 10 minutes, and each supernatant and precipitate were liquid scintillation counters (LSC; Liquid Scintillation). Counter (CPM) to obtain a value of CPM (Count Per Minute), based on the following equation 1, the control group and the experimental group collagen biosynthesis ratio (RCB; Relative Collagen Biosynthesis) was measured and the results are shown in Table 3 below. .

(weight%) Indongcho vinegar extract
(Production Example 1) Indongcho Extract
(Comparative Production Example 1) Collagen biosynthesis ratio (RCB)
(%) Control - - 100 Example 2 10 - 152 Comparative Example 3 - 10 120

In the results of Table 3, in the case of Comparative Example 3 treated with the common Indongcho extract, the collagen biosynthesis ratio was shown to be about 120% compared to the control, whereas in the case of Example 2 treated with the Indongcho vinegar extract, the collagen biosynthesis ratio of 152% compared to the control It was found to show a remarkably excellent efficacy.

[Test Example 3] Sensory evaluation of lifting efficacy

For 20 women aged 25-35 who are suffering from sagging skin, use the lotion of Example 1 and Comparative Examples 1 to 2 in the same way as normal lotion for 4 weeks, and then start using them every 1 week. The user's skin lifting efficacy was evaluated through a questionnaire according to the following evaluation criteria. The results are shown in Table 4 below.

<Evaluation criteria>

+++: Very good improvement.

++: Significant improvement.

+: Slightly improved.

±: No improvement effect, but no deterioration.

-: No improvement effect, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 weeks Example 1 ++ +++ +++ +++ Comparative Example 1 ± + + + Comparative Example 2 + ++ ++ ++

In the results of Table 4, compared to Comparative Example 1 containing no extract at all, Comparative Example 2 containing a general extract of Indongcho, Example 1 containing the extract of Indongcho vinegar according to the present invention shows rapid and excellent lifting efficacy. Did.

[Test Example 4] Efficacy in reducing pore size

23 women in their 30s and older who have trouble with pores were selected, and Example 1 was applied daily to the place where the pores were prominent for 2 weeks, and then skin touch was performed every 1 week before and after the test was started (manufacturer: True System) The pore size was measured through the pore analysis program, and the average pore size was divided into 3 grades by the pore analysis program, and the number of each group was counted and the results are shown in Table 5 below.

division Average pore size
(pixel) Pore size
Large group
(persons) Pore size
Middle group
(persons) Pore size
Small group
(persons) Before exam 195.3 9 10 4 After 1 week of testing 185.0 8 9 6 After 2 weeks of testing 162.3 7 8 8

From the results of Table 5, it can be seen that the composition according to the present invention can effectively reduce the size of the pores.

[Test Example 5] Sensory evaluation of pore shrinking effect

For 20 women aged 25-35 who are suffering from large pores, the cosmetic lotions of Examples 1 and 2 and 2 were used in the same way as normal lotion for 4 weeks, respectively, and the degree of skin improvement was improved according to the user's opinion. The results are shown in Table 6 below by making the determination according to the following evaluation criteria.

<Evaluation criteria>

+++: Very good improvement.

++: Significant improvement.

+: Slightly improved.

±: No improvement effect, but no deterioration.

-: No improvement effect, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 weeks Example 1 ++ +++ +++ +++ Comparative Example 1 + + ++ ++ Comparative Example 2 + + ++ +++

In the results of Table 6, compared to Comparative Example 1 containing the extract of Indongcho vinegar and Comparative Example 2 containing the extract of Indongcho vinegar, Example 1 containing the extract of Indongcho vinegar showed rapid and excellent pore shrinking effect.

[Test Example 6] Confirmation of the effect of reducing sebum by the extract of Indongcho vinegar

Ten males and females in their 20s and 30s who felt that there was a lot of sebum secretion were selected, and Example 1 was applied daily to a lot of sebum on the face for 4 weeks. Before starting the test and 2 weeks after the test, 4 weeks after the test, the sebum amount was measured using a skin oil meter (Sebumeter SM810), and the results are shown in Table 7 below.

Subject Before test (㎍ / ㎠h) After 2 weeks of testing
(㎍ / ㎠h) After 4 weeks of testing
(㎍ / ㎠h) One 101 93 85 2 85 86 80 3 86 80 72 4 101 94 86 5 94 85 79 6 74 66 60 7 65 51 45 8 62 51 50 9 90 82 76 10 59 55 47

From the results of Table 7, it can be seen that the composition according to the present invention can effectively inhibit sebum.

[Test Example 7] Sensory evaluation of acne improvement effect

Among the 19-26 year old women, 20 people with acne were used to use the lotion of the above Example 1 and Comparative Examples 1 to 2 in the same way as normal lotion for 4 weeks, respectively, and the degree of skin improvement was improved according to the user's opinion. It judged according to the following evaluation criteria. The results are shown in Table 8 below.

<Evaluation criteria>

+++: Very good improvement.

++: Significant improvement.

+: Slightly improved.

±: No improvement effect, but no deterioration.

-: No improvement effect, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 weeks Example 1 ++ +++ +++ +++ Comparative Example 1 ± ++ + ++ Comparative Example 2 + ++ ++ ++

In the results of Table 8, Example 1 containing the extract of Indongcho vinegar compared to Comparative Example 1 that does not contain the extract of Indongcho vinegar and Comparative Example 2 containing the extract of Indongcho vinegar showed rapid and excellent acne skin improvement effect. .

Claims (2)

A composition for external application for skin for acne improvement, comprising an extract of vinegar from Indongcho. According to claim 1,
The phosphorus vinegar extract is an external composition for skin, characterized in that it contains 0.001 to 90.0% by weight relative to the total weight of the composition.