KR102070662B1 - Skin external composition comprising Lonicera japonica vinegar extract - Google Patents

Abstract

The present invention relates to an external preparation composition for skin containing dongdongcho vinegar extract, and more particularly, to an external preparation composition for skin having excellent skin elasticity, pore reduction, sebum reduction and acne-improving effect.

Description

Skin external composition comprising Lonera japonica vinegar extract

The present invention relates to an external preparation composition for skin containing dongdongcho vinegar extract, and more particularly, to an external preparation composition for skin having excellent skin elasticity, pore reduction, sebum reduction and acne-improving effect.

Vinegar is a kind of seasoning that tastes sour. Since it is produced through acetic acid fermentation, vinegar has been used from high gold as a stored food containing acetic acid. These vinegars are largely classified into brewed vinegar and synthetic vinegar according to the manufacturing method, and brewed vinegar is made by brewing method. Can be. Synthetic vinegar is manufactured by a chemical process, and includes pure synthetic vinegar and vinegar added to the synthetic vinegar.

Indongcho, also known as gold and silver, is a perennial vine plant belonging to the Indong family. It is distributed mainly in Korea and East Asia. It has antipyretic, detoxification, and sweating effects and has been used for the treatment of colds, etc., and has been used as a treatment for infectious hepatitis and boils.

As described in the present invention, there is no research or report on an excellent effect on skin when acetic acid is fermented with acetic acid and used as a vinegar extract.

Korean Patent Publication No. 10-2004-21589

The present inventors have found that Indong vinegar vinegar extract exhibits a remarkably excellent effect on skin elasticity, pore reduction, sebum reduction and acne improvement effect and completed the present invention.

Accordingly, it is an object of the present invention to provide a skin external preparation composition containing the extract of Indongcho vinegar to exhibit skin elasticity, pore reduction, sebum control and acne improving effect.

In order to achieve the above object, the present invention provides a skin external preparation composition for enhancing skin elasticity containing the extract of Indong vinegar.

The present invention also provides a skin external preparation composition for reducing pores, containing the extract of Indongcho vinegar.

The present invention also provides a skin external preparation composition for sebum reduction containing Indongcho vinegar extract.

In addition, the present invention provides an external preparation composition for improving acne containing the extract of Indongcho vinegar.

The composition according to the present invention can contain the skin extract of Indongcho vinegar to provide skin elasticity, pore reduction, sebum reduction and acne-improving effect, in particular, compared to the general Indongcho extract, skin elasticity, pore reduction, sebum reduction and acne-improving effect The external preparation composition for skin which shows remarkably excellent efficacy can be provided.

The external preparation composition for skin according to the present invention is Indongcho ( Lonicera) japonica ; Honeysuckle) Contains vinegar extract as an active ingredient.

Indongcho used in the present invention is also known as gold and silver, is a perennial vine plant belonging to the Indong family, mainly distributed in East Asia, including Korea. It has antipyretic, detoxification, and sweating effects and has been used for the treatment of colds, etc., and has been used as a treatment for infectious hepatitis and boils.

Lonicera in the present invention japonica ) can use all parts of the plant such as roots, stems, leaves, and flowers. It can also be used as a whole plant (outpost). Phosphorus vinegar extract according to the present invention means obtained by acetic acid fermentation of the phosphorus vinegar. In addition, the extract of Indong vinegar can be prepared through the saccharification process, alcohol fermentation process and acetic acid fermentation process.

In one embodiment of the present invention, the phosphorus vinegar extract may be contained in an amount of 0.001% by weight or more based on the total weight of the composition, preferably in an amount of 10.0% by weight or more. In another embodiment of the present invention, the phosphorus vinegar extract may be contained in an amount of 90.0% by weight or less based on the total weight of the composition, preferably in an amount of 70.0% by weight. In another embodiment of the present invention, the Indong vinegar vinegar extract may be contained in an amount of 0.001% by weight or more and 90.0% by weight or less based on the total weight of the composition, more preferably 10.0% by weight or more and 70.0% by weight or less It may contain. When it is used at less than 0.001% by weight, its efficacy may not be sufficiently exhibited, and when it is used at more than 90.0% by weight, the pH is low, which may cause irritation.

The composition according to the present invention can be used as an external preparation composition for skin elasticity. It is excellent in promoting the collagen synthesis of the skin, improve the skin elasticity can improve the skin sag and provide a lifting effect.

The composition according to the present invention can be used as a skin external preparation composition for pore reduction, sebum control, which is excellent in the effect of suppressing excessive secretion of sebum when applied to the skin and reducing pore size.

The composition according to the present invention can be used as an external preparation composition for improving acne, has an excellent antibacterial effect against acne causing bacteria, and has an excellent skin trouble suppressing effect.

The composition according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) and non-obtained by dispersing an oil phase in solution, gels, solids, pasty anhydrous products, aqueous phases. It may be provided in the form of an ionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or concealed stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions can be prepared according to conventional methods in the art.

In addition, the composition according to the present invention is a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or nonionic. Such as type emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics. It may contain adjuvants conventionally used in the cosmetic or dermatological field. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological fields.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to test examples and formulation examples. However, these test examples and formulation examples are provided only for the purpose of illustration to help the understanding of the present invention is not limited to the scope and scope of the present invention by the following examples.

Preparation Example 1 Preparation of Indongcho Vinegar Extract

1. Base liquor manufacturing process (glycosylation process)

After washing 20 kg of brown rice, immersed in 30 kg of bottled water (160m rock water) for 5 hours, drain and prepare water, and extract wild cabbage, extract and prepare water by mixing 9 kg of brown rice and indong vinegar. The mixture is steamed at a temperature of ˜110 ° C. for 25 to 35 minutes and then cooled to 60 ° C. 2 kg of malt powder is added to the cooled brown rice and saccharified for 12 hours. 6 kg of wheat malt was added thereto, and the yeast was activated by saccharifying at a temperature of 30 ° C. for 48 hours. Then, the obtained rice was put in a jar and 40 kg of bottled water (160 m rock water) was added to air at 25 ° C. for 72 hours. The base liquor is prepared by blocking and keeping only carbon dioxide out.

2. Alcohol Fermentation Process

After washing 13kg of brown rice, immersed in 20kg of water for 1 hour, drained water and 1kg of the remaining brown rice and Indongcho outpost to increase the temperature at a temperature of 90 ~ 110 ℃ 25 to 35 minutes to prepare a rice paddy. Put the prepared rice and Indongcho outpost 9kg in the base liquor prepared in 1. and alcohol fermentation at 25 ℃ for 10-15 days.

3. Acetic acid fermentation process

After diluting 1 kg of water into the fermentation product obtained in 2. and sealing the inlet with a cloth so that air enters the jar at 27 ° C. for 2 months, supplying oxygen while stirring occasionally to ferment the primary (aerobic) acetic acid . After measuring the acidity of the obtained fermented product (PH-230SD PH meter) to confirm that the pH is 4 or more, only the supernatant is separated and aged in a shaded cool place of 5 ~ 20 ℃ second. After that, the aged liquid was filtered to obtain an extract of Indongcho vinegar. The resulting Indongcho vinegar extract was sterilized, bottled and stored in a cool dark place, which was provided for the following test.

Comparative Preparation Example 1 Preparation of Indongcho Extract

Indongcho extract of the present invention was mixed with 10g butylene glycol, 71g purified water to 19g Indongchocho outpost and extracted by heating for 1 hour. Thereafter, the mixture was centrifuged at 8000 rpm and 15 ° C. for 30 minutes, and then filtered sequentially with a 3.00 μm, 1.2 μm, 0.7 μm, and 0.45 μm filter. The extracted stock solution was suspended in an auto mixer at 800 rpm for 1 hour to contain 10% of butylene glycol, thereby obtaining a phosphorus extract.

Example 1 and Comparative Examples 1 and 2

Using Preparation Example 1 and Comparative Preparation Example 1, Example 1 and Comparative Examples 1 and 2 were prepared according to the composition of Table 1 below (unit: wt%).

Raw material name Example 1 Comparative Example 1 Comparative Example 2 One Indongcho vinegar extract (manufacturing example 1) 78 - - 2 Indongcho extract (Comparative Preparation Example 1) - - 78 3 Purified water To 100 To 100 To 100 4 Disodium ID 0.02 0.02 0.02 5 glycerin 5.0 5.0 5.0 6 Xanthan Gum 0.15 0.15 0.15 7 Methylgluses-20 2.0 2.0 2.0 8 Glyceres-26 2.0 2.0 2.0 9 Ammonium Acryloyl Dimethyl Taurate / V-Picopolymer 0.3 0.3 0.3 10 ethanol 3.0 3.0 3.0 11 Hydrogenated Castor Oil 0.3 0.3 0.3 12 incense 0.1 0.1 0.1 13 antiseptic 0.3 0.3 0.3

<Production method>

(1) The aqueous phase components (1-9) were uniformly mixed at room temperature.

(2) The ethanol phase components 10-13 were heated to 50-55 ° C. to be uniformly dissolved and mixed.

(3) The said (1) was thrown into the said (2) under stirring, and it was solubilized uniformly.

Test Example 1 Skin Elasticity Improvement Effect

In order to confirm the skin elasticity improving effect according to the present invention was tested as follows using Example 1 and Comparative Examples 1 and 2. After dividing 80 women in their 30's and 40's into 3 sets, each of Example 1, Comparative Example 1, and Comparative Example 2 were applied to the face twice daily (morning and evening) for 4 weeks, and then, a skin elasticity measuring instrument (Cutometer) SEM 575, C + K Electronic Co., Germany) to measure the skin elasticity improvement effect, the results are shown in Table 2 below. The results in Table 2 refer to the properties of skin viscoelasticity of the skin elasticity tester.

Test substance Skin elasticity effect Example 1 0.66 ± 0.10 Comparative Example 1 0.30 ± 0.09 Comparative Example 2 0.42 ± 0.09

In the results of Table 2, Comparative Example 2 containing the general Indongcho extract showed a skin elasticity enhancing effect of about 140% compared to Comparative Example 1. However, in the case of using Example 1 containing the Indongcho vinegar extract exhibited a remarkably excellent effect by showing a skin elasticity enhancement effect of about 220% compared to Comparative Example 1.

Test Example 2 Collagen Synthesis Promoting Effect

Human fibroblasts were cultured in a 24-well plate incubator, and then replaced with a medium containing the Indong vinegar extract of Preparation Example 1 and the general Indong vinegar extract of Comparative Preparation Example 1 at the concentrations of Table 3 below. On the third day of culture, 0.5 ml of DMEM medium containing 10% fetal calf serum was added to each well, followed by 10 μCi of L [2,3,4,5- ³ H] -proline. After 24 hours, the media and cells in each well are scraped, washed in 5% trichloroacetic acid (TCA) solution, dispensed into two test tubes, and one type I cola in one test tube. 1 unit / μl of genase (type I collagenase) was added thereto, and incubated at 37 ° C. for 90 minutes, and the other test tubes were stored at 4 ° C. Thereafter, 0.05 ml of 50% trichloroacetic acid was added to all test tubes, and the mixture was left at 4 ° C. for 20 minutes, and then centrifuged at 12,000 rpm for 10 minutes, respectively. CPM (Count Per Minute) value was obtained, and the collagen biosynthesis ratio (RCB; Relative Collagen Biosynthesis) was measured for the control group and the experimental group according to Equation 1 below, and the results are shown in Table 3 below. .

(weight%) Indongcho Vinegar Extract
(Manufacture example 1) Indongcho Extract
(Comparative Production Example 1) Collagen Biosynthesis Rate (RCB)
(%) Control - - 100 Example 2 10 - 152 Comparative Example 3 - 10 120

In the results of Table 3, Comparative Example 3 treated with the general Indongcho extract showed a collagen biosynthesis ratio of about 120% compared to the control, whereas Example 2 treated with Indongcho vinegar extract showed a collagen biosynthesis ratio of 152% compared to the control. It can be seen that the markedly excellent efficacy.

Test Example 3 Sensory Evaluation of Lifting Efficacy

For 20 women aged 25 to 35 who are suffering from skin sagging, the skin lotion of Example 1 and Comparative Examples 1 and 2 were used for the same method as general lotion for 4 weeks, and then at 1 week intervals after the start of use. The degree of skin lifting efficacy of the user was evaluated through a questionnaire according to the following evaluation criteria. The results are shown in Table 4 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: Slight improvement.

±: No improvement but no deterioration.

-: No improvement, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 Weeks Example 1 ++ +++ +++ +++ Comparative Example 1 ± + + + Comparative Example 2 + ++ ++ ++

In the results of Table 4, compared to Comparative Example 1 containing no extract at all, Comparative Example 2 containing a general Inchocho extract extract Example 1 containing the extract of Indongcho vinegar according to the present invention shows a quick and excellent lifting efficacy It was.

Test Example 4 Pore Size Reduction Efficacy

23 women over 30 years old with pores were selected and corrected in Example 1 every day where the pores were prominent for 2 weeks, and then skin touched before and after the start of the test (manufacturer: True Systems). The pore size was measured by the pore analysis program. The average pore size was divided into three grades by the pore analysis program, and the number of each group was shown in Table 5 below.

Average pore size
(pixel) Pore size
Large group
(persons) Pore size
Middle class
(persons) Pore size
Small group
(persons) Before the test 195.3 9 10 4 1 week after the exam 185.0 8 9 6 2 weeks after the test 162.3 7 8 8

In the results of Table 5, it can be seen that the composition according to the present invention can effectively reduce the size of the pores.

Test Example 5 Sensory Evaluation of Pore Reduction Effect

For 20 women aged 25 to 35 years who are suffering from large pores, the skin lotion of Example 1 and Comparative Examples 1 and 2 was used in the same manner as general lotion for 4 weeks, and according to the opinion of the user, the degree of skin improvement To be determined according to the following evaluation criteria are shown in Table 6 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: Slight improvement.

±: No improvement but no deterioration.

-: No improvement, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 Weeks Example 1 ++ +++ +++ +++ Comparative Example 1 + + ++ ++ Comparative Example 2 + + ++ +++

In the results of Table 6, compared to Comparative Example 1 containing no Indongcho vinegar extract and Comparative Example 2 containing a general Inchochocho extract, Example 1 containing the Inchochocho vinegar extract showed a quick and excellent pore shrinkage effect.

[Test Example 6] Confirmation of sebum reduction effect by the extract of Indongcho vinegar

Ten male and female subjects in their twenties and thirties who felt that there was a lot of sebum secretion were selected, and Example 1 was corrected every day for a lot of sebum on the face for four weeks. Before the start of the test and after 2 weeks of testing, after 4 weeks of testing, sebum was measured using a skin oil analyzer (Sebumeter SM810), and the results are shown in Table 7 below.

Subject Before test (㎍ / ㎠h) 2 weeks after the test
(Μg / cm 2 h) 4 weeks after the test
(Μg / cm 2 h) One 101 93 85 2 85 86 80 3 86 80 72 4 101 94 86 5 94 85 79 6 74 66 60 7 65 51 45 8 62 51 50 9 90 82 76 10 59 55 47

In the results of Table 7, it can be seen that the composition according to the present invention can effectively inhibit sebum.

Test Example 7 Sensory Evaluation of Acne Improvement Effect

20 women of 19 to 26 years of age with acne were to use the lotion of Example 1 and Comparative Examples 1 and 2 in the same manner as the general lotion for 4 weeks, respectively, according to the opinion of the user to improve the skin It judged according to the following evaluation criteria. The results are shown in Table 8 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: A slight improvement.

±: No improvement but no deterioration.

-: No improvement, rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 Weeks Example 1 ++ +++ +++ +++ Comparative Example 1 ± ++ + ++ Comparative Example 2 + ++ ++ ++

In the results of Table 8, compared to Comparative Example 1 containing no Indongcho vinegar extract and Comparative Example 2 containing a general Inchocho extract, Example 1 containing Indongcho vinegar extract showed a quick and excellent acne skin improvement effect .

Claims (9)

As a skin external composition for enhancing skin elasticity containing an extract of Indongcho vinegar as an active ingredient,
The indong vinegar extract,
(a) saccharifying the cauliflower to prepare a base liquor;
(b) increasing the Indongcho to prepare rice;
(c) adding fermented rice prepared in step (b) and indongcho to the base liquor prepared in step (a) to obtain fermented product by alcoholic fermentation; And
(d) acetic acid fermentation of the fermentation product obtained in step (c) to obtain an extract of Indongcho vinegar
The skin external preparation composition for skin elasticity enhancement, which is obtained through a manufacturing process comprising a. The method of claim 1,
The content of the Indongcho vinegar extract is characterized in that 0.001 to 90.0% by weight based on the total weight of the composition, the skin external composition for skin elasticity enhancement. delete delete delete delete delete delete delete