KR102282645B1 - Cosmetic composition comprising mixed extracts of cnidium officinale and scutellaria baicalensis or compounds seperated from thereof for alleiviating acne and dandruff - Google Patents

Abstract

The present invention is Cnidium officinale ) and gold ( Scutellaria ) baicalensis ) It relates to a cosmetic composition for alleviating acne and dandruff containing as active ingredients Senkyunolide A and Wogonin, which are mixed extracts or separated compounds thereof. The cosmetic composition of the present invention can inhibit acne bacteria, has an effect of inhibiting lipid biosynthesis, dandruff alleviating effect, and inhibiting sebum secretion.

Description

COSMETIC COMPOSITION COMPRISING MIXED EXTRACTS OF CNIDIUM OFFICINALE AND SCUTELLARIA BAICALENSIS OR COMPOUNDS SEPERATED FROM THEREOF FOR ALLEIVIATING ACNE AND DANDRUFF}

The present invention relates to a cosmetic composition for alleviating acne and dandruff, comprising as active ingredients a mixed extract of chrysanthemum and gold, or senqunolide A and ugonin, which are separated compounds thereof.

In general, sebum in the skin including the scalp and face plays a role in maintaining moisture in the skin and preventing the invasion of microorganisms, but it causes acne after puberty. In particular, in the case of women in their twenties or later, excessive secretion of sebum causes many problems in terms of beauty, such as oiliness of the skin, lifting of makeup, and enlarged pores. Various causes are involved in the occurrence of excess sebum, the most important of which is that the cells of the sebaceous gland are activated by the amount of dihydrotestosterone, which is one of the hormones involved in promoting the secretion of sebum in the activity of the sebaceous gland, and the hypersecretion of sebum occurs. is known

Acne is not a single cause, but multiple causes are involved. The hyperfunction of male hormones during puberty leads to an active secretion of sebaceous glands and abnormalities in the epithelial cells of the hair follicles, which block the hair follicles, resulting in the formation of comedones, the basic lesion of acne. do. Bacteria residing in hair follicles stimulate the hair follicles by producing free fatty acids, thereby causing inflammation. This progression to acne is further exacerbated by the hypersecretion of sebum. The sebaceous gland promotes lipid synthesis by generating androgens and increasing the expression of enzymes involved in lipid synthesis, and related enzymes include Fatty acid synthase, acetyl-CoA carboxylase, ATP-citrate lyase, and the like. Enzymes related to lipid metabolism have an electronic regulatory site called SRE, and androgens are known to regulate enzymes related to lipid synthesis by regulating SREBP binding to SRE. In addition, the production of free fatty acids promotes the formation of colonies and cells of acne bacteria, thereby causing skin inflammation and thickening the keratin.

For acne treatment, conventionally, female hormones such as estrogen or 5α-reductase inhibitors have been used. However, these female hormones have been reported to cause skin inflammation, side effects due to hormone administration, etc., in addition to the acne treatment effect, and are currently discontinued or used in very small amounts. Although hypersecretion of sebum in the cosmetic field is an important subject of interest as a factor that causes cosmetic lifting or enlarged pores, studies on it are insignificant. Currently, sebum suppression cosmetics use porous powder that temporarily adsorbs sebum, or use ivy extract, a herbal extract known to suppress sebum, as cosmetic raw materials, but the effect is insignificant and safe natural extract-derived sebum secretion There is a need to develop a cosmetic composition having an effect of alleviating acne symptoms by suppressing the

Korean Patent Registration No. 10-1212346 discloses a cosmetic composition having anti-acne treatment and prevention effects, including extracts from cheongung and Dol et al.

In addition, US Patent Publication No. 2018/0078481 discloses a compound extracted from celery (apium graveolens ) while disclosing a topical cosmetic composition for anti-dandruff treatment.

The present inventors have search results for a variety of skin bioactive natural plant ingredients to improve acne, horoscope from natural plant extracts (Cnidium officinale ) and gold (Scutellaria baicalensis ) From the extract to confirm the efficacy, and to complete the present invention by confirming its active ingredient. Accordingly, an object of the present invention is to provide a cosmetic composition having an effect of alleviating acne and dandruff, including a mixed extract of cheongung and gold.

The above purpose is the Cnidium officinale ) and gold ( Scutellaria baicalensis ) It is achieved by a cosmetic composition for alleviating acne and inhibiting sebum secretion, comprising a mixed extract of an active ingredient.

Preferably, the cosmetic composition comprises a mixture of Senkyunolide A isolated from cheongung and Wogonin isolated from gold as an active ingredient.

Also preferably, the cosmetic composition has an effect of inhibiting sebum production.

Also preferably, the mixed extract may include the cheongung extract and the golden extract in a weight ratio of 1:1 to 1:2, and the mixture contains senqunolide A and ugonin in a weight ratio of 1:1 to 5:1. can be included as

Also preferably, the mixed extract of cheongung and gold may be included in an amount of 0.01 to 30.0% by weight based on the total weight of the cosmetic composition.

Also preferably, the mixture of Senqunolide A and ugonin may be included in an amount of 0.001 to 5.0% by weight based on the total weight of the cosmetic composition.

Also preferably, the cheongung extract and the golden extract may be extracted with an extraction solvent selected from the group consisting of water, anhydrous or hydrated lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate and butyl acetate.

The cosmetic composition according to the present invention contains a mixed extract of chrysanthemum and gold or its separated compounds, Senkyunolide A and wogonin, as active ingredients to provide excellent acne relief, dandruff relief, and sebum secretion. has an inhibitory effect.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art in the relevant field of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include the steps or elements presented, or groups of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention is Cnidium officinale ) and Gold ( Scutellaria baicalensis ) relates to a cosmetic composition comprising a mixed extract as an active ingredient.

Cnidium used in the present invention officinale ) is a perennial herbaceous plant belonging to the Umbrella family, and is often cultivated for medicinal purposes in Korea. The medicinal properties of cheongung are mild and sour, and have effects such as blood circulation, pain relief, and activation of the liver function. In oriental medicine, leaves and roots have been used for soothing, analgesic, and tonic effects for a long time.

Gold used in the present invention ( Scutellaria baicalensis ) is the root of rotten grass, a cone-shaped twisted root with a length of 8-30 cm and a diameter of 1-4 cm. The outer surface of the root is yellowish brown or dark yellow. Rotten grass is commonly found in domestic production, and has been used for high blood pressure, headache, and hemostasis of nosebleeds since ancient times, and has been widely used for symptoms of reducing fever due to its golden cold properties.

Cnidium of the present invention (Cnidium officinale ) extract and gold ( Scutellaria ) baicalensis ) extract can be prepared according to a conventional method known in the art, that is, under conventional temperature and pressure conditions, using a conventional solvent, but preferably (a) water, having 1-4 carbon atoms. Anhydrous or hydrous lower alcohols (e.g., methanol, ethanol, propanol and butanol) , propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and a solvent extraction method using a solvent selected from the group consisting of a mixture thereof (b) a supercritical extraction method using reduced pressure and high temperature with carbon dioxide, or (c) an ultrasonic extraction method. In the present invention, preferably, a solvent extraction method using an extraction solvent was used.

Cnidium in the present invention (Cnidium officinale ) extracts and gold ( Scutellaria baicalensis ) extracts are various extraction solvents, for example, water, anhydrous or hydrated lower alcohols having 1-4 carbon atoms (eg, methanol, ethanol, propanol and butanol), propylene glycol, 1, 3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, and can be obtained using at least one extraction solvent selected from the group consisting of mixtures thereof, preferably is obtained using ethanol, 70% (v/v) ethanol or water, and most preferably obtained using 70% (v/v) ethanol.

On the other hand, Cnidium of the present invention (Cnidium officinale ) extract and gold ( Scutellaria baicalensis) extract, it is apparent to those skilled in the art that an extract having substantially the same effect can be obtained using other extraction solvents as well as the above-described extraction solvent.

In addition, the horoscope of the present invention (Cnidium officinale ) extracts and gold ( Scutellaria baicalensis) extracts include extracts that have undergone conventional purification and fermentation processes, as well as extracts by the above-mentioned extraction solvent. For example, reduced pressure by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by extraction using ultrasound, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity Active fractions obtained through various additional purification and extraction methods, such as separation by means of separation by sex) or extracts by fermentation products using natural conditions or various microorganisms, are also included in the extract of the present invention.

Cnidium according to the invention (Cnidium officinale ) extract and gold ( Scutellaria baicalensis ) extract also includes an extract that has undergone a fermentation process, and the fermented extract can be prepared as follows. Firmament (Cnidium officinale ) root and golden ( Scutellaria baicalensis ) root ( 100 ~ 500 mesh finely crushed, then 1 ~ 50 g / L of a conventional microbial culture medium is added and microorganisms such as yeast strains or lactic acid bacteria of 10,000 ~ 100,000 cfu / L It is added in an amount.Cultivation is carried out under normal microbial culture conditions of 30-37° C. Incubated for about 5 to 10 days under aerobic or normally anaerobic conditions with a pH of 5-7. After maturation and It can be obtained through filtration.

According to the invention, the horoscope (Cnidium officinale) and gold (mixed extract of Scutellaria baicalensis) is contained 0.01 to 30.0 wt% based on the total weight of the cosmetic composition, and more preferably, the horoscope (Cnidium officinale ) and gold (Scutellaria baicalensis ) extract is characterized in that it is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. Also preferably, the mixed extract of Cnidium (Cnidium officinale), and gold (Scutellaria baicalensis) is a Cnidium extract, Gold extract 1 may include a weight ratio of 2: 1 to 1.

When the content of the mixed extract is less than 0.01% by weight, the skin improvement effect does not appear, and when it exceeds 30.0% by weight, there is a problem in safety and stability of the formulation, and it is not economical.

According to one embodiment of the present invention, when the cosmetic composition includes a mixture of senqunolide A and ugonin, the mixture is preferably included in an amount of 0.001 to 5.0% by weight based on the total weight of the cosmetic composition. Also preferably, the mixture may include senqunolide A and ugonin in a weight ratio of 1:1 to 5:1.

The cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredient. conventional adjuvants such as, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide is used as a carrier component. can be

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 fatty acid esters of ,3-butylglycol oil, glycerol fatty esters, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether as carrier components Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

On the other hand, the makeup method of the present invention refers to all makeup methods using the cosmetic composition of the present invention. That is, all methods known in the art using the cosmetic composition belong to the cosmetic method of the present invention. The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

The following preparation examples include the above-mentioned (a) water, anhydrous or hydrous lower alcohols having 1-4 carbon atoms (eg, methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin , acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and a solvent extraction method using a solvent selected from the group consisting of mixtures thereof, (b) supercritical extraction method by reduced pressure and high temperature with carbon dioxide, Alternatively, it is apparent to those skilled in the art that an extract having substantially the same effect can be obtained even by using (c) ultrasonic extraction and (d) fermentation process, and it is not limited to the preparation examples mentioned below.

In the present invention, the active ingredient, Chunkyung extract, includes a whole plant or root extract, and the active ingredient, senquinolide A, is a compound isolated from the Chunkyung extract and is represented by the following formula (1).

[Formula 1]

In the present invention, the golden extract as an active ingredient includes a root extract, and the active ingredient, ugonin, is a compound isolated from the golden extract and is represented by the following formula (2).

[Formula 2]

In the present invention, an "extract" is an extract obtained by extraction treatment of chrysanthemum or gold, and a dilution of the extract, a concentrate, a dried product obtained by drying it, a prepared product of the extract, a purified product, or any form that can be formed using the extract. It means the extract of the formulation.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples according to the gist of the present invention.

Preparation 1 - Cnidium (Cnidium officinale ) extract preparation

After washing the roots of chrysanthemum with clean water, drying them, cut them to a certain size, extract under reflux three times for 5 hours each with 70% (V/V) ethanol aqueous solution, cool, and filter with Whatman #4 filter paper to obtain a concentrated solution .

Preparation 2 - Gold ( Scutellaria baicalensis ) extract preparation

The golden root was washed with clean water and dried, cut to a certain size, extracted under reflux three times for 5 hours each with 70% (V/V) ethanol aqueous solution, cooled and filtered with Whatman #4 filter paper to obtain a concentrated solution

Preparation Example 3 - Preparation of Senquinolide A

1L of purified water was added to the cheongung extract obtained in Preparation Example 1 and dispersed, followed by solvent fractionation using n-hexane. This fractionated layer was concentrated under reduced pressure to obtain 10.27 g. The hexane layer was re-fractionated with chloroform and methanol (20:9) to obtain 4.47 g of a non-polar fraction layer. The obtained non-polar fraction layer was separated and purified using a silica gel (60~120mesh) column, and the mobile phase was separated and purified by controlling the conditions of hexane and methanol (40:1 -> 1:1). was recovered, and 0.337 g of the most effective fraction was recovered using prep-LC through evaluation of the efficacy of each fraction, and the structure was identified through NMR, and the literature (ZSCHOCKE, S., et al. HPLC-coupled spectroscopic techniques (UV) , MS, NMR) for the structure elucidation of phthalides in Ligusticum chuanxiong.　Molecular diversity, 2005, 9.1-3: 33-39.) and it was confirmed that the isolated component was Senquinolide A. The chemical formula of senqunolide A is shown in Formula 1 above.

Preparation Example 4 - Preparation of Ugonin

After dispersing the extract obtained in Preparation Example 2 by adding 1 L of purified water, solvent fractionation was performed using n-hexane and ethyl acetate. The ethyl acetate fractional layer was concentrated under reduced pressure to obtain 107.44 g. The ethyl acetate fraction was re-fractioned with chloroform, methanol, and purified water (10:5:1) to obtain 29.14 g of the fractionated layer. The obtained fractional layer was re-verified through efficacy evaluation, and the structure was identified through NMR after purification using prep-LC. DELANGE, David Marrero, et al. Selective and high yield isolation of pure wogonin from aerial parts of Scutellaria havanensis Jacq. Int. J. Pharm. Sci. Rev. Res, 2015, 30.2: 104-108.) and standards and By comparison, it was confirmed that the separated component was ugonin. Ugonin's chemical formula is shown in Formula 2 above.

Experimental example 1 - Measurement of antibacterial activity by disc-diffusion method

In order to confirm the antimicrobial activity of the extract of the present invention prepared through the above examples and the active ingredient isolated therefrom, a disk diffusion method was used.

50ul of Preparation Examples 1 and 2 of a certain concentration were dispensed on a sterilized paper disk, and the solvent was removed through natural drying at room temperature for 24 hours. Cultured P. acnes And M. furfur was spread by dispensing 1 ml each with the same initial number of bacteria on a plate medium for disk diffusion method measurement. Since then, the paper disc is P. acnes And M. furfur was applied to the smeared plate medium, and then incubated for 48 hours at an appropriate temperature, and then the size of the growth inhibition ring (Clean Zone) was measured. The results are shown in Tables 1 to 3 below.

Sample name Concentration (ppm) Early number of bacteria growth Ji Hwan
(mm) Preparation Example 1
(Chungung extract) 20,000 1.07*10 ⁷
( P. acnes ) 12.33 10,000 10.50 1,000 - Preparation 2
(Golden Extract) 20,000 14.55 10,000 12.33 100 - Preparation 3
(Senquinolide A) 1,000 8.93 100 - Preparation 4
(Ugonin) 1,000 - 100 - Preparation Example 1
(Chungung extract) 20,000 1.51*10 ⁷
( M. furfur ) 12.50 10,000 9.50 1,000 - Preparation 2
(Golden Extract) 20,000 9.33 10,000 - 100 - Preparation 3
(Senquinolide A) 1,000 - 100 - Preparation 4
(Ugonin) 1,000 9.26 100 -

division Early number of bacteria growth Jeo Ji-hwan (mm) Preparation Example 1
(Chungung extract)
10,000ppm Preparation 2
(Golden Extract) 20,000ppm 1.21*10 ⁷
( P. acnes ) 17.50 10,000ppm 14.33 Preparation Example 1
(Chungung extract)
10,000ppm Preparation 2
(Golden Extract) 20,000ppm 1.34*10 ⁷
( M. furfur ) 14.50 10,000ppm 12.00

division Early number of bacteria growth Jeo Ji-hwan (mm) Preparation 3
(Senquinolide A)
1,000ppm Preparation 3
(Ugonin) 1,000ppm 1.12*10 ⁷
( P. acnes ) 14.33 100ppm - 1,000ppm 1.09*10 ⁷
( M. furfur ) 12.88 100ppm -

Experimental example 2 - Lipid biosynthesis inhibitory effect

Acne and dandruff are caused by several factors. In particular, acne is caused by an excess of male hormones, leading to active secretion of sebaceous glands and clogging of hair follicles, resulting in the formation of comedones, the basic lesion of acne. In the case of dandruff, hyperkeratinization and inflammatory reactions occur due to dandruff bacteria that proliferate excessively by using excessively secreted sebum as a nutrient. Therefore, regulation of lipid biosynthesis can improve both acne and dandruff. After heat was applied to human sebocytes, proteins were quantified and antibodies were fixed, and then the expression of proteins (FAS, SREBP-1, PPARγ) was checked and quantified using c-digit. The results are shown in Tables 4 and 5.

division density
(ppm) FAS inhibition rate ( % ) SREBP -One PPARγ inhibition rate ( % ) Preparation Example 1
(Chungung extract) 50 21.24
±1.27 20.47
±0.91 23.27
±2.07 Preparation 2
(Golden Extract) 50 18.42
±2.17 15.94
±3.22 20.97
±1.02 Preparation 3
(Senquinolide A) 50 32.68
±2.29 28.81
±0.97 35.35
±2.52 10 19.21
±1.49 9.24
±1.51 12.63
±2.19 One 8.17
±0.76 - - Preparation 3
(Ugonin) 50 17.33
±2.24 22.31
±2.11 15.17
±1.31 10 7.92
±0.87 8.67
±1.17 - One - - -

division FAS inhibition rate ( % ) SREBP -One PPARγ inhibition rate ( % ) Preparation 3
(Senquinolide A)
50ppm Preparation 3
(Ugonin) 50ppm 43.10
±1.00 35.71
±2.18 45.11
±2.01 10ppm 25.94
±1.25 21.46
±2.13 23.49
±1.19 1ppm 17.07
±1.37 9.47
±0.92 10.19
±2.12

Experimental example 3 - cholesterol level measurement

^{Sebum cells were dispensed 1 X 10 5} each in a 10 cm culture vessel, and each of Preparation Examples 1 to 4 was treated at a concentration of 1, 10, and 50 μg/ml, respectively, and cultured for 5 days and cholesterol was quantified. For the cholesterol quantification method, the cells cultured for 5 days were treated with a lysis buffer (EDTA 10 μL, 100 mM SPB solution, PMSF 1 μL, Triton X-100 10 μL) and then lysed with ultrasound. A chloroform:methanol (2:1) solution was added to the cell lysate, mixed, and then centrifuged (2000rpm, 15 minutes) to take the lower layer and concentrated with _{nitrogen gas (N 2 ).} After dissolving 300 μL of ethanol in the concentrated sample, the amount of cholesterol was measured using a total cholesterol kit (AM 202-K). 100 μL of sample was added to 1 mL of enzyme solution, mixed, and incubated at 37° C. for 5 minutes, and then absorbance was measured with an ELISA reader at a wavelength of 500 nm. Cholesterol in the standard solution was set to a value of 3 mg/mL by adding 20 uL of the standard solution to 3 mL of the enzyme solution. The results are shown in Tables 6 and 7.

density
(μg/ml) cholesterol
(ml/ml protein) control - 0.97 Preparation Example 1 (Chungung Extract) 50 0.91 Preparation Example 2 (Golden Extract) 50 0.90 Preparation 3
(Senquinolide A) 50 0.80 10 0.83 One 0.86 Preparation 4
(Ugonin) 50 0.78 10 0.82 One 0.85

division cholesterol
(ml/ml protein) control - 0.94 Preparation 3
(Senquinolide A)
50 μg/ml Preparation 4
(Ugonin) 50 μg/ml 0.63 10 μg/ml 0.79 1 μg/ml 0.81 Preparation 3
(Senquinolide A)
10 μg/ml 50 μg/ml 0.68 10 μg/ml 0.72 1 μg/ml 0.79

Through the above experimental examples, it can be confirmed that the combination use of senqunolide A and ugonin isolated from cheongung and gold has a superior cholesterol production inhibitory effect than the single use.

The following formulation examples are examples of formulations containing a mixture of Senqunolide A and Ugonin, and the scope of the present invention is not limited by the formulation examples mentioned below.

prescription example 1 - lotion

A formulation example of a lotion among cosmetics containing a mixture containing 50 ppm of Senkyunolide A and 50 ppm of Ugonin is shown in Table 8 below.

ingredient content
(weight%) Formulation Example 1 Comparative Formulation Example 1 Senquinolide A and Ugonin Mixture
glycerin
1.3-Butylene Glycol
PG 1500
allantoin
DL-Panthenol
EDTA-2Na
Benzophenone-9
Sodium Hyaluronate
ethanol
Octic dodeces-16
Polysorbate 20
preservatives, fragrances, colors
Distilled water -
glycerin
1.3-Butylene Glycol
PG 1500
allantoin
DL-Panthenol
EDTA-2Na
Benzophenone-9
Sodium Hyaluronate
ethanol
Octic dodeces-16
Polysorbate 20
preservatives, fragrances, colors
Distilled water 3.0
5.0
3.0
1.0
0.1
0.3
0.02
0.04
5.0
10.0
0.2
0.2
a very small amount
remaining amount Sum 100

prescription example 2 - cream

A formulation example of a cream among cosmetics containing 50 ppm of Senquinolide A and 50 ppm of Ugonin is shown in Table 9 below.

ingredient Formulation Example 2
(weight%) Comparative Formulation Example 2
(weight%) Senquinolide A and Ugonin Mixture
Lipophilic Nostearate Glycerin
cetearyl alcohol
stearic acid
beeswax
Polysorbate 60
Sorbitan Stearate
hydrogenated vegetable oil
squalane
mineral oil
trioctanoin
dimethicone
Sodium Magnesium Silicate
glycerin
betaine
triethanolamine
Sodium Hyaluronate
preservatives, fragrances, colors
Distilled water 3.0
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
remaining amount -
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
remaining amount Sum 100

prescription example 3 - Essence

Table 10 below shows the prescription examples of the essence in the cosmetics containing 50 ppm of Senqunolide A and 50 ppm of Ugonin.

ingredient content
(weight%) Formulation Example 3 Comparative Formulation Example 3 Senquinolide A and Ugonin Mixture
glycerin
betaine
Fiji 1500
allantoin
DL-Panthenol
E.D.T.A-2Na
Benzophenone - 9
Hydroxyethyl Cellulose
Sodium Hyaluronate
Carboxyvinyl Polymer
triethanolamine
Octyldodecanol
Octyldodeces -16
ethanol
preservatives, fragrances, colors
Distilled water -
glycerin
betaine
Fiji 1500
allantoin
DL-Panthenol
E.D.T.A-2Na
Benzophenone - 9
Hydroxyethyl Cellulose
Sodium Hyaluronate
Carboxyvinyl Polymer
triethanolamine
Octyldodecanol
Octyldodeces -16
ethanol
preservatives, fragrances, colors
Distilled water 3.0
10.0
5.0
2.0
0.1
0.3
0.02
0.04
0.1
8.0
0.2
0.18
0.3
0.4
6.0
a very small amount
remaining amount Sum 100

Example 1 - Sebum secretion inhibitory effect of a cosmetic containing a mixed extract and an active ingredient

The experiment was conducted using the cream formulations of Formulation Example 2 and Comparative Formulation Example 2 prepared in Formulation Example 2, and 20 men and women in their 20s were selected for each group, and mixed extracts (Senqunolide A 50ppm and Ugo Nin 50ppm) containing the cream of Formulation Example 2, and in group B, the cream of Comparative Formulation Example 2 that did not contain the cheongung extract was used twice a day in the morning and evening for 4 weeks after cleansing. Sebum measurement was measured after washing the face for 30 minutes in a constant temperature and humidity room, and then using a sebumeter to measure the amount of sebum on the forehead. Before using the cream for the test, the amount of sebum was set to the initial value, and the change in the amount of sebum was measured once a week for 4 weeks while continuously using the softening lotion. The change in the amount of sebum by the cheongung extract of the present invention was compared with the initial amount of sebum to determine whether or not the sebum inhibitory effect was achieved, and the results are shown in Table 11.

Sebum secretion (g/cm2/h) Formulation Example 2
group A Comparative Formulation Example 2
group B initial value 243 ± 12.7 214 ± 10.29 2 weeks 190 ± 11.4 201 ± 12.11 4 weeks 174 ± 10.1 204 ± 9.11

The cream of Formulation Example 2 containing the active ingredients separated from the extracts of cheongung and goldenrod of the present invention effectively reduced sebum secretion over time, and suppressed sebum secretion compared to Comparative Formulation Example 2 containing nothing. was found to be excellent. Therefore, it is expected that the mixture of the present invention (senqunolide A 50 ppm and ugonin 50 ppm) can effectively reduce sebum secretion, thereby preventing acne from occurring due to excessive sebum secretion.

Example 2 - Irritation relief effect of a cosmetic containing a mixed extract and active ingredient

The experiment was carried out using the cream formulations of Formulation Example 3 and Comparative Example prepared in Formulation Example 2, and 20 men and women in their twenties were selected for each group, and a mixed extract (Senqunolide A and Ugonin 100ppm) in group A. The cream of Formulation Example 3 containing this, and the cream of Comparative Formulation Example that did not contain Chunkyung extract in group B, were used twice a day, in the morning and in the evening, for 4 weeks after cleansing. For evaluation, it was evaluated whether irritation appeared on the skin while using it for 4 weeks using both sides of the face.

division irritation relief effect no irritation mild irritation strong sense of stimulation Formulation Example 2
group A 10 8 2 Comparative Formulation Example 2
group B 5 7 8

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (8)

A mixture of Senkyunolide A isolated from Cnidium officinale and Wogonin isolated from Scutellaria baicalensis in a weight ratio of 1:1 to 5:1 as an active ingredient A cosmetic composition for alleviating acne and dandruff. According to claim 1, wherein the cosmetic composition has a sebum production inhibitory effect, acne and dandruff relief cosmetic composition. The cosmetic composition for alleviating acne and dandruff according to claim 1, wherein the mixture of senqunolide A and ugonin is included in an amount of 0.001 to 5.0% by weight based on the total weight of the cosmetic composition. delete delete delete delete delete