KR102221627B1 - Composition comprising Rhus Semialata extract as active ingredient - Google Patents

Abstract

The present invention provides a new use of the redwood extract. The present invention provides a skin moisturizing use and/or a skin wrinkle improvement use of the redwood extract. The redwood extract of the present invention can be used as a cosmetic ingredient, a pharmaceutical ingredient, or a health food ingredient that is safe for the skin and has excellent skin condition improvement effect.

Description

Composition comprising Rhus Semialata extract as active ingredient {Composition comprising Rhus Semialata extract as active ingredient}

The present invention relates to a composition comprising a redwood extract, and more specifically to a composition for improving skin wrinkles or enhancing skin moisturizing effect.

Elastin fibers form cross-links with collagen and are an important skin component for wrinkle formation, which is involved in skin elasticity. Deficiency and aggregation of elastin fibers, and a marked increase in the activity of elastin-degrading enzyme, Elastase, have been found to be one of the factors of skin wrinkles. Elastase is the only enzyme that can break down elastin, and its inhibition is known to fundamentally reduce skin wrinkles.

Elastin fiber is a matrix protein that plays an important role in retaining moisture in the dermal layer. These matrix proteins adsorb moisture and increase moisture retention in the structures that they form, allowing the skin to maintain a state containing adequate moisture. It is known to be involved in maintaining the elasticity of the skin.

In addition, elastic fibers produced by fibroblasts of the skin form cross-links with collagen and are an important skin component for wrinkle formation, which is involved in skin elasticity. The deficiency and decomposition of elastic fibers was found to be one of the major factors in skin wrinkle formation and elasticity loss.

Currently, retinoids, adenosine, animal placenta-derived proteins, and chlorella extracts are known as wrinkle-improving cosmetics. Retinol, the most well-known substance, promotes collagen synthesis and inhibits elastase enzyme, but it is unstable. When applied to the skin, the amount of use is limited due to safety issues such as irritation and redness, and chlorella extract is practically ineffective for the skin. It is known that it is difficult to expect a wrinkle improvement effect.

Meanwhile, Rhus Semialata ) is a low acidity?鰥? It lives and is named because the leaves are dyed very red in autumn. It is also known as a salt tree because it is characterized by its salty taste and seems to open salt.

Redwood has different medicinal effects in each area. From ancient times, the fruit of the red tree is called Yeombuja. It is known to have the effect of removing inflammation, stopping diarrhea, stopping coughing, stopping bleeding, and preventing sweating. The leaves are called salted leaves and are used for detoxification when a snake bites them. The stem bark is called Yeombusubaekpi, and it treats bloody diarrhea and itchy skin.

In addition, Obaeja refers to a worms house formed by aphids (Aphis chinensis J. Bell) on the leaves of a red tree, and a kind of aphid is parasitic to create an insect hump, which is distributed throughout Korea. It is known that Obaeja does not have the same composition and efficacy as redwood.

On the other hand, when the skin connective tissue becomes loose, skin elasticity decreases and wrinkles are caused. To prevent this, vitamin A or C was treated as a method of promoting collagen synthesis or inhibiting collagen degradation. However, these components are easily decomposed by the external environment, and there is a problem that they cause skin irritation. In addition, cosmetics containing hyaluronic acid directly have a moisturizing effect on the skin surface, but there is a problem of being rapidly decomposed by hyaluronic acid decomposing enzymes in the skin.

Republic of Korea Public Publication No. 10-2015-0001334 (released on January 6, 2015)

Accordingly, the object of the present invention is to provide a cosmetic composition for moisturizing skin that solves the above problems, inhibits the activity of hyaluronic acid decomposing enzyme, and improves the moisturizing effect of the skin.

In addition, the present invention is to provide a cosmetic composition for improving skin wrinkles that improves skin wrinkles and enhances the elasticity of the skin.

In order to solve the above problem, the present invention is Rhus tree (Rhus Semialata ) provides a composition containing the extract as an active ingredient. In one embodiment, it provides a composition for improving skin wrinkles and/or a composition for moisturizing skin, including redwood extract. In one embodiment, the present invention is to provide a cosmetic composition for skin moisturizing and/or a cosmetic composition for improving skin wrinkles, comprising a redwood extract as an active ingredient. In one embodiment, it is intended to provide a cosmetic composition for moisturizing skin and/or a cosmetic composition for improving skin wrinkles, including a seed and/or root extract of redwood. In another embodiment, to provide a cosmetic composition for skin moisturizing and/or a cosmetic composition for improving skin wrinkles comprising a seed extract of redwood.

The inventors of the present invention intend to provide a new use of a red tree extract, preferably a red tree seed and/or root extract, more preferably a red tree seed extract, for wrinkle improvement and/or skin moisturizing use.

In the present invention, "improving elasticity or skin elasticity" means reducing the degree of sagging or sagging of the skin. In addition, the elasticity means maintaining the elasticity of the skin in a state in which sufficient elastin and collagen are present.

In the present invention, the term "wrinkle improvement" refers to preventing, inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating the wrinkles that have already been created.

In the present invention, the term "moisturizing or moisturizing the skin" means increasing the hydration feeling to the skin and maintaining a moist state. The skin moisturizing effect can help improve skin wrinkles and increase elasticity.

In particular, the inventors of the present invention tested using the root extract and seed (seed) extract of the red tree, and the root and seed extract of the red tree compared to other sites, and more preferably the seed extract of the red tree is strong Elastase. The present invention was completed by confirming that it exhibits inhibitory activity and hyaluronidase inhibitory activity. In addition, it was confirmed that the ethanol extract of seeds is more stable to heat (and light) and pH than the root extract, and is suitable for use as a raw material for cosmetics.

The redwood extract of the present invention refers to an extract extracted by a conventional method in the field to which the present invention belongs, and may be further purified by a method such as fractionation, separation, and filtration after extraction.

For example, the redwood extract according to the present invention may be a solvent extract thereof or a dried product thereof. When extracting redwood, the extraction solvent may be used without limitation as long as it is a solvent used for extracting natural products. For example, purified water; Lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; Hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or they may use a mixed solvent, but it is preferable to use a lower alcohol having 1 to 4 carbon atoms such as purified water, methanol, ethanol, propyl alcohol, and butyl alcohol, or a mixed solvent thereof, and purified water, ethanol or a mixed solvent thereof It is more preferable to use, and it is more preferable for the purposes of the present invention to use a mixed solvent of purified water and ethanol.

The amount of the extraction solvent may be 10 to 100 times the weight of redwood, preferably 20 to 80 times, and more preferably 30 to 50 times, but is not limited thereto.

The method of extracting the extract may use conventional extraction methods in the art such as stirring extraction, cold needle extraction, hot water extraction, immersion extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, reflux cooling extraction, or ultrasonic extraction. It may be heated and extracted under reflux or at room temperature, preferably. The extraction temperature is preferably 10 to 30 ℃, the extraction time is preferably 1 to 20 days, more preferably 1 to 5 days, but is not limited thereto.

More specifically, after the redwood is pulverized in the shade, the extraction solvent is added and extracted by 1 to 20 times the volume of the redwood crushed product, and then optionally (depressurized) concentration, drying or purification may be performed. More specifically, the active ingredient is extracted by adding an extraction solvent as described above, but (a) heating at a temperature of 50 to 100° C. for 1 to 20 hours while a cooling condenser is installed to prevent evaporation of the solvent. The active ingredient may be extracted or (b) immersed for 1 to 15 days at a temperature of 5 to 37°C.

Further, according to another embodiment according to the present invention, the red tree extract may be a fraction of the red tree extract.

The redwood fraction of the present invention may be obtained by fractionating the redwood extract (preferably a lower alcohol extract having 1-4 carbon atoms) with a solvent having a lower polarity than water such as hexane, chloroform, butanol and ethyl acetate, and preferably hexane Alternatively, chloroform may be used, but is not limited thereto.

In particular, for the purpose of achieving the effect according to the present invention, the redwood extract according to the present invention is preferably a hexane or chloroform fraction of a lower alcohol extract having 1-4 carbon atoms.

The fractions are more polar than water such as hexane, chloroform, butanol or ethyl acetate after using a liquid red oak extract before drying or suspending or dissolving the dried red oak extract in a suitable solvent such as water, ethanol or a mixed solvent thereof. This low solvent can be added sequentially to separate each layer, and then each layer can be obtained by concentrating and/or drying under reduced pressure.

At this time, the vacuum concentration is preferably using a vacuum rotary evaporator, but is not limited thereto.

In addition, "drying" according to the present invention may be vacuum drying, vacuum drying, boiling drying, spray drying, room temperature drying, or freeze drying, but it is more preferable to perform freeze drying. However, the present invention is not limited to this drying method.

The redwood extract of the present invention may be included in an amount of 0.0001 to 20% by weight based on the total weight of the composition, preferably 0.0005 to 5% by weight, and even more preferably 0.001 to 1% by weight.

When the content of the extract is less than 0.0001% by weight, it is difficult to expect elastase inhibition and hyaluronidase inhibitory activity, and when it exceeds 10% by weight, a distinct effect is not expected as much as the increasing content.

The redwood extract of the present invention can be prepared as a cosmetic composition according to a method for preparing a cosmetic composition containing a conventional herbal extract.

In addition, the cosmetic composition according to the present invention may include ingredients that are commonly used in addition to the extract, for example, a moisturizer, a thickener, a surfactant, an emulsifier, an antiseptic, an antioxidant, an alcohol, a fragrance, a pH adjuster, a natural extract, etc. Of course, a chemical component for doubling the effect of improving dark circles by the extract can be blended in an appropriate amount.

The cosmetic composition according to the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, soaps, surfactant-containing cleansing, oils, etc. It may be formulated as, but is not limited thereto. More specifically, in the form of a flexible lotion, nourishing lotion, nourishing cream, emulsion, cosmetic ointment, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, gel, makeup base, foundation, spray or powder. Can be manufactured.

As a preferred formulation of the present invention, a lotion, cream, and essence formulation in which the extracts are evenly dispersed in the cosmetic composition and the effects of the present invention are best exhibited are preferred.

The present invention also provides a method for enhancing skin elasticity, improving skin wrinkles, or moisturizing skin, comprising the step of applying the redwood extract to the skin of an individual. The subject includes, without limitation, mammals including mice, livestock, humans, and the like.

According to another embodiment of the present invention, the present invention is Rhus Semialata ) to provide a pharmaceutical composition containing the extract as an active ingredient. Specifically, the present invention is to provide a pharmaceutical composition for skin moisturizing and/or a pharmaceutical composition for improving skin wrinkles comprising a redwood extract as an active ingredient. Even more specifically, the present invention is to provide a pharmaceutical composition for skin moisturizing and/or a pharmaceutical composition for improving skin wrinkles, including a seed and/or root extract of redwood. More specifically, the present invention is to provide a pharmaceutical composition for skin moisturizing and/or a pharmaceutical composition for improving skin wrinkles, comprising a seed extract of redwood.

The composition containing the compound of the present invention may be in various oral or parenteral formulations, but preferably may be a parenteral formulation. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations include at least one excipient in one or more compounds, such as starch, calcium carbonate, sucrose, or lactose ( lactose), gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups.In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as humectants, sweeteners, fragrances, and preservatives may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, and emulsions. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used.

The present invention can also be provided in a formulation of a skin external preparation for skin elasticity improvement, skin wrinkle improvement, or skin moisturization comprising a redwood extract as an active ingredient.

When using the redwood extract as an external preparation for skin, additionally fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water , Ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, lipid vesicles, or commonly used in external preparations for skin It may contain adjuvants commonly used in the field of dermatology such as any other ingredients that are used. In addition, the ingredients may be introduced in an amount generally used in the field of dermatology.

When the redwood extract is provided in a formulation for external application for skin, it is not limited thereto, but may have a formulation such as an ointment, patch, gel, cream, or spray.

The pharmaceutical composition of the present invention may be particularly preferably used as a parenteral preparation. For example, the external preparation for skin may include a suitable pharmaceutically acceptable base such as petrolatum and stearyl alcohol; Suitable pharmaceutically acceptable surfactants such as polysorbate and sorbitan sesquioleate; Pharmaceutically acceptable suitable moisturizers such as glycerin; Suitable pharmaceutically acceptable solvents; And a flavoring agent, a colorant, a stabilizer, a viscous agent, and the like are homogeneously mixed.

In addition, when using the redwood extract of the present invention as a pharmaceutical, it may further contain one or more active ingredients exhibiting the same or similar function. For example, it may include known ingredients for improving skin elasticity, skin wrinkles, or skin moisturizing. When the additional skin elasticity enhancement, skin wrinkle improvement, or skin moisturizing component is included, the skin elasticity enhancement, skin wrinkle improvement, or skin moisturizing effect of the composition of the present invention may be further enhanced. When the above ingredients are added, skin safety, ease of formulation, and stability of active ingredients can be considered in combination with use.

When the pharmaceutical composition of the present invention contains an effective amount of the redwood extract, it can provide desirable skin elasticity enhancement, skin wrinkle improvement, or skin moisturizing effect. In the present invention, the term "effective amount" means an amount of a compound capable of sufficiently exhibiting skin elasticity enhancement, skin wrinkle improvement, or skin moisturizing effect.

The effective amount of the redwood extract contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, how the compound is applied to the skin, and the time it stays on the skin. For example, when the composition is commercialized as a pharmaceutical, it may contain the redwood extract in a higher concentration than when it is commercialized as a cosmetic that is routinely applied to the skin. Therefore, the daily dosage is 0.1 to 100 mg/kg, preferably 30 to 80 mg/kg, more preferably 50 to 60 mg/kg, based on the amount of the compound of Formula 1, and 1 to It can be administered 6 times.

The composition containing the redwood extract of the present invention exhibits excellent effects on skin elasticity improvement, skin wrinkle improvement, or skin moisturization.

The composition containing the redwood extract of the present invention can be used as a cosmetic ingredient or pharmaceutical ingredient that is safe for skin and has excellent skin condition improvement effect.

1 is a result of confirming the inhibitory effect of hyaluronidase activity for each part of the red tree.

Hereinafter, in order to describe the present invention in more detail, it will be described with reference to the following examples. However, the embodiments according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. Embodiments of the present invention are provided by way of example to aid in a specific understanding of the present invention.

Preparation of red tree root and seed extract

The extracts for each part of the red tree used in the following examples were obtained from China 花有限公司 (Muyang huaxiang zhongye Co., Ltd.), and then 10 times of ethanol was added and the extract was heated at 60°C for 4 hours.

[ Example 1) Redwood Root Extract ( Rhus Semialata Root extract) and red tree seed extract ( Rhus Semialata Seed extract) hyaluronidase inhibitory activity

50 mL of hyaluronidase (1 ng/mL) (R&D systems; 7358-GH-020) was placed in a microtube, 50 mL of an evaluation agent was added, and incubated at 25°C for 1 hour. As an evaluation agent, Rhus root extract (Rhus Semialata Root extract) and Rhus seed extract (Rhus Semialata Seed extract) was used, and the untreated group to which nothing was added and Gallotannin (Santa Cruz Biotechnology; sc-202619) were added as a positive control for comparison of effects. Red tree root extract and red tree seed extract were added so that the concentration after addition was 0.4 ppm, 10 ppm, and 250 ppm, respectively, and Gallotannin was added to 850 ppm. After incubation, the hyaluronidase activity ELISA (Echelon; K-6000) was decomposed by the activity of hyaluronidase at each concentration and the amount of remaining hyaluronic acid was measured at 450 nm using a spectrophotometer. . Table 2 and Fig. 1 show the results of setting the hyaluronidase activity of the untreated group to 100.

sample Hyaluronic acid (ng/ml) Hyaluronidase activity relative to control (%) Control (untreated group) 0.32 100 Gallotannin 850 ppm 3.96 11.80034 Rhus Semialata Stem extract 250 ppm 1.43 73.10395 Rhus Semialata Leaf extract 250 ppm 0.88 86.43082 Rhus Semialata Root extract 0.4 ppm 1.2 78.67701 Rhus Semialata Root extract 10 ppm 2.433 48.80058 Rhus Semialata Root extract 250 ppm 4.117 7.996123 Rhus Semialata Seed extract 0.4 ppm 1.41 73.58856 Rhus Semialata Seed extract 10 ppm 2.965 35.90986 Rhus Semialata Seed extract 250 ppm 4.332 2.786528

As shown in Table 1 and FIG. 1, when inhibiting the activity of hyaluronidase by 90% or more by gallotannin, which is a positive control, red tree root extract and red tree seed extract inhibit the activity of hyaluronidase in a concentration-dependent manner. Can be seen. At this time, it was confirmed that the hyaluronidase inhibitory activity of the stem and leaf extracts of the red tree was significantly lower than that of the root and seed extracts.

[ Example 2] Redwood Root Extract ( Rhus Semialata Root extract) and red tree seed extract ( Rhus Semialata Seed extract) Ela Stays ( Elastase ) Inhibitory activity

To investigate the other functions of redwood root and seed extract, 100 mUnit/mL unit elastase and 0.5 mM MeOSuc-Ala-Ala-Pro-Val as a substrate of elastase in 100 mM Tris-HCl buffer (pH 7.5). -p-nitroanalide (Bachem; L-1335) was added and reacted at 25° C. for 20 minutes, and absorbance was measured at 405 nm to quantify the amount of p-nitroanilide produced from the substrate. EGCG was used as a control group.

Rhus Semialata Seed Extract showed 87.15% of elastatase inhibitory activity at a concentration of 2 ppm. Rhus Semialata Root Extract showed 33.15% elastatase inhibitory activity at a concentration of 2 ppm. It can be seen that the redwood seed extract exhibited an elastatase inhibitory activity at a lower concentration than the root extract, which showed similar activity to EGCG (Epigallocatechin gallate), which showed 96.23% of elastatase inhibitory activity. At this time, Rhus Semialata Stem Extract and Rhus Semialata Leaf Extract were 21.09% and 15.28%, respectively, at a concentration of 200 ppm, indicating that their elastase inhibitory activity was significantly lower than that of root and seed extracts. I can.

sample Elastase activity (%) Elastase activity inhibition rate (%) Control (untreated group) 100 - EGCG 1 ppm 3.77 96.23 Rhus Semialata Stem extract 200 ppm 78.91 21.09 Rhus Semialata Leaf extract 200 ppm 84.72 15.28 Rhus Semialata Seed extract 200 ppm 9.23 90.77 Rhus Semialata Seed extract 100 ppm 8.41 91.59 Rhus Semialata Seed extract 50 ppm 9.64 90.36 Rhus Semialata Seed extract 10 ppm 12.54 87.46 Rhus Semialata Seed extract 2 ppm 12.85 87.15 Rhus Semialata Seed extract 0.4 ppm 88.27 11.73 Rhus Semialata Root extract 200 ppm 4.13 95.87 Rhus Semialata Root extract 100 ppm 4.49 95.51 Rhus Semialata Root extract 50 ppm 7.24 92.76 Rhus Semialata Root extract 10 ppm 31.41 68.59 Rhus Semialata Root extract 2 ppm 66.85 33.15 Rhus Semialata Root extract 0.4 ppm 74.71 25.29

[ Manufacturing example ] Cream manufacturing

As shown in the composition of Table 3 below, a cream containing a red tree seed extract was prepared by a conventional method. In addition, a cream prepared with the remaining ingredients excluding the active ingredient was prepared in Comparative Example 1.

Composition (% by weight) Manufacturing Example 1 Comparative Example 1 Red tree seed extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Appropriate amount Appropriate amount incense Appropriate amount Appropriate amount Purified water to 100 to 100

[ Example 3] Confirmation of skin wrinkle improvement effect through panel test

For the creams of Preparation Example 1 and Comparative Example 1, the skin wrinkle improvement effect was tested for healthy 35 to 50 year old women as follows.

30 women aged 35 to 50 years old were divided into two groups of 15 each, group 1 was the cream of Example 1 containing 1% by weight of redwood seed extract, and group 2 was the cream of Comparative Example 1 on the face. It was applied once a day for 3 months. After 3 months, the degree of improvement of skin wrinkles was evaluated through a questionnaire of subjects and image analysis of skin wrinkles. The subject's questionnaire was evaluated in three stages of improvement in skin wrinkles and elasticity, no improvement, slight improvement, and significant improvement compared to before use, and the results are shown in Table 4 below.

Category (person) No improvement Slight improvement Significant improvement Manufacturing Example 1 One 7 7 Comparative Example 1 10 4 One

As can be seen in Table 4, when the cream of Preparation Example 1 containing redwood seed extract is used, it can be confirmed that the skin wrinkle improvement effect is superior to the case of using the cream of Comparative Example 1 that is not used.

Claims (10)

It contains redwood extract as an active ingredient,
The red tree extract is a cosmetic composition for improving skin wrinkles, which is a root extract, a seed extract, or a mixture thereof. delete The cosmetic composition for improving skin wrinkles according to claim 1, wherein the red tree extract is a seed extract of red tree. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the red tree extract is contained in an amount of 0.0001 to 10% by weight based on the total weight of the composition. delete It contains redwood extract as an active ingredient,
The red tree extract is a cosmetic composition for moisturizing the skin of a red tree root extract, seed extract, or a mixture thereof. delete The cosmetic composition for moisturizing skin according to claim 6, wherein the red tree extract is a seed extract of red tree. The cosmetic composition for moisturizing skin according to claim 6, wherein the redwood extract is contained in an amount of 0.0001 to 10% by weight based on the total weight of the cosmetic composition. delete

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