KR20180055137A - Composition comprising Rhus Semialata extract as active ingredient - Google Patents

Abstract

The present invention provides a new use of a Rhus semialata extract. The present invention provides a skin moisturizing and/or skin wrinkle-reducing use of Rhus semialata extract. The Rhus semialata extract of the present invention can be used as a raw material for cosmetics, a pharmaceutical ingredient, or a health food, which is safe for skin and has excellent skin condition improving effect. The Rhus semialata extract is a Rhus semialata root extract, a Rhus semialata seed extract, or a mixture thereof.

Description

Composition comprising Rhus verniciflua extract as an active ingredient [

More particularly, the present invention relates to a composition for improving skin wrinkles or skin moisturizing effect.

Elastin fibers form crosslinks with collagen and are an important skin component in wrinkle formation that is involved in skin elasticity. The deficiency and aggregation of elastin fibers and the marked increase in the activity of the elastin degrading enzyme Elastase have been found to be one of the causes of skin wrinkles. It is known that Elastase is the only enzyme capable of degrading elastin, and its inhibition can fundamentally reduce the wrinkles of the skin.

Elastin fiber is a substrate protein that plays an important role in moisture retention in the dermal layer. These protein proteins adsorb moisture and increase the water holding capacity inside the structure, thereby enabling the skin to maintain proper moisture content. It is known to be involved in maintaining the elasticity of the skin.

In addition, the elastic fiber produced in the fibroblast of the skin forms a cross-link with collagen, and is a skin constituent important for wrinkle formation which is involved in skin elasticity. Deficiency and decomposition of elastic fibers were found to be one of the main factors of skin wrinkle formation and elasticity reduction.

Presently, retinoids, adenosine, animal placenta-derived proteins, chlorella extract, and the like are known to improve wrinkles. The best known retinol promotes collagen synthesis and inhibits elastase enzymes, but it is unstable. When applied to the skin, it has a limited use due to safety problems such as irritation and redness, and chlorella extract has little effect, It is known that it is difficult to expect a wrinkle-improving effect.

Meanwhile, Rhus Semialata ) is a low - It is a name that the leaf is very reddish when it falls in the fall, and it is characterized by the salty taste in the fruit that opens in the autumn. It is also called the salt tree by the nickname.

The rhusin has different medicinal effects for each part. From ancient times, it has been known that the fruit of a rhus major is called a sprout, which has the effect of eliminating inflammation, stopping diarrhea, stopping coughing, stop bleeding, and prevent perspiration. Leaves are called bamboo leaf, and they are used for deciphering when they are bitten by a snake. The stem skin is said to have hundreds of saliva, but it treats diarrhea and skin itching mixed with blood.

Aphis chinensis J. Bell is a house of insects caused by stings on the leaves of rhus chinensis. It is a kind of aphid which is parasitized to make insect hump and distributed in various parts of Korea. It is known that rhubarb is not the same in composition and efficacy as rhusi.

On the other hand, when the skin connective tissue is loosened, skin elasticity is lowered and wrinkles are induced. To prevent this, vitamin A or C is treated by promoting collagen synthesis or inhibiting collagen degradation. However, these components are easily decomposed by the external environment and cause skin irritation. In addition, the cosmetic composition in which hyaluronic acid is directly blended exhibits a moisturizing effect on the surface of the skin, but has a problem of being rapidly decomposed by hyaluronic acid degrading enzyme in the skin.

Korean Laid-Open Publication No. 10-2015-0001334 (Published May 1, 2015)

Accordingly, an object of the present invention is to provide a cosmetic composition for skin moisturizing which solves the above problems, inhibits the activity of hyaluronic acid degrading enzyme and improves the moisturizing effect of the skin.

The present invention also provides a cosmetic composition for improving the wrinkles of the skin and improving skin elasticity.

In order to achieve the foregoing object, the present invention sumac (Rhus Semialata extract as an active ingredient. In one embodiment, there is provided a composition for improving skin wrinkles and / or a composition for skin moisturizing comprising Rhodiola extract. In one embodiment, the present invention provides a cosmetic composition for moisturizing skin and / or a cosmetic composition for improving skin wrinkle comprising rhus chinense extract as an active ingredient. In one embodiment, the present invention provides a cosmetic composition for moisturizing skin and / or a cosmetic composition for improving skin wrinkles containing seed and / or root extract of rhusin. In another embodiment, there is provided a cosmetic composition for moisturizing skin and / or a cosmetic composition for improving skin wrinkles containing seed extract of rhusin.

The inventors of the present invention intend to provide a new use for the wrinkle-improving and / or skin moisturizing application of rhusi extract, preferably seed and / or root extract of rhusi, and more preferably seed extract of rhusi.

In the present invention, the term 'improving elasticity or skin elasticity' means reducing the degree to which the skin is stuck or stretched. In addition, the elasticity means that elasticity of the skin is maintained in a state where elastin and collagen are sufficiently present.

In the present invention, "improving wrinkles" refers to preventing, suppressing or inhibiting the generation of wrinkles on the skin, or alleviating wrinkles that have already been formed.

In the present invention, the term 'moisturizing or skin moisturizing' means increasing the skin moisture and maintaining a moist state. Skin moisturizing effect can help to improve wrinkles and elasticity of skin.

In particular, the inventors of the present invention have found that the extracts of rhus chinensis and seeds are more effective than those of other parts of rhusin, and more preferably the extracts of rhus chinensis have a stronger elasticity than that of other parts, Inhibitory activity and hyaluronidase inhibitory activity, and completed the present invention. In addition, the ethanol extract of seeds was found to be more stable to heat (and light) and pH than roots extract, and thus it was confirmed to be suitable as a raw material for cosmetics.

The rhusin extract of the present invention means an extract obtained by a conventional method in the field of the present invention and can be further purified by extraction, fractionation, separation, filtration and the like.

For example, the rhusin extract according to the present invention may be a solvent extract thereof or a dried product thereof. The extraction solvent for the extraction of rhusin may be any solvent used for extracting natural products without limitation, for example, purified water; Lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; Hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether and dichloromethane; Or a mixed solvent thereof may be used, but it is preferable to use purified water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, butyl alcohol, or a mixed solvent thereof, and it is preferable to use purified water, ethanol, , And it is more preferable for the purpose of the present invention to use a mixed solvent of purified water and ethanol.

The amount of the extracting solvent may be 10 to 100 times, preferably 20 to 80 times, more preferably 30 to 50 times the weight of rhusi, but is not limited thereto.

The method of extracting the above extract may be carried out by a conventional extraction method such as stirring, cold extraction, hot water extraction, immersion extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, And it is preferable to heat and reflux or extract at room temperature. The extraction temperature is preferably 10 to 30 ° C, and the extraction time is preferably 1 to 20 days, more preferably 1 to 5 days, but is not limited thereto.

More specifically, the rhusin is shredded and shredded, the extraction solvent is added by 1 to 20 times the volume of the crushed rhea powder, extracted, and then selectively (decompression) concentrated, dried or purified. More specifically, an extraction solvent is added to extract an active ingredient substance as in the above-mentioned method, (a) heating at a temperature of 50 to 100 ° C for 1 to 20 hours in a state where a cooling condenser is installed to prevent evaporation of the solvent, (B) immersing the mixture at a temperature of 5 to 37 DEG C for 1 to 15 days to extract the active ingredient.

According to another embodiment of the present invention, the rhus chinense extract may be a fraction of Rhus verniciflua extract.

The rhus chinensis fraction of the present invention can be obtained by fractionating the rhusi extract (preferably a lower alcohol extract having 1 to 4 carbon atoms) with a solvent having a lower polarity than water such as hexane, chloroform, butanol and ethyl acetate, Or chloroform, but is not limited thereto.

In particular, for the purpose of attaining the effect of the present invention, the rhusin extract according to the present invention is preferably a hexane or chloroform fraction of a lower alcohol extract having 1-4 carbon atoms.

The fractions may be obtained by suspending or dissolving the dried rattan extract in a suitable solvent such as water, ethanol, or a mixed solvent thereof, using a liquid rattan extract before drying, This low solvent may be sequentially added to separate each layer, and then each layer may be obtained by concentration under reduced pressure and / or drying.

At this time, the vacuum concentration is preferably performed using a vacuum rotary evaporator, but not limited thereto.

In addition, " drying " according to the present invention may be vacuum drying, vacuum drying, boiling drying, spray drying, room temperature drying or freeze-drying, However, the present invention is not limited to this drying method.

The rhusin extract of the present invention may be contained in an amount of 0.0001 to 20% by weight, preferably 0.0005 to 5% by weight, and more preferably 0.001 to 1% by weight based on the total weight of the composition.

When the content of the extract is less than 0.0001% by weight, it is difficult to expect the inhibition of the elastase and the hyaluronidase inhibitory activity. When the content of the extract is more than 10% by weight, the effect of increasing the content is not expected.

The rumen extract of the present invention can be prepared as a cosmetic composition according to a method for producing a cosmetic composition containing an ordinary herbal extract.

In addition, the cosmetic composition according to the present invention may contain components commonly used in addition to the above extracts. For example, moisturizers, thickeners, surfactants, oil bases, preservatives, antioxidants, alcohols, fragrances, Of course, a chemical component for doubling the dark circles improvement effect by the above extract can be blended in an appropriate amount.

The cosmetic composition according to the present invention may be prepared in any form conventionally produced in the art, and may be in the form of solution, suspension, emulsion, paste, gel, cream, lotion, soap, surfactant- , But is not limited thereto. More specifically, the present invention relates to a cosmetic lotion composition for use in cosmetics, such as a soft lotion, a nutritional lotion, a nutritional cream, a latex, a cosmetic ointment, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a gel, a makeup base, .

A preferred formulation of the present invention is a lotion, cream, or essence formulation in which the extracts are evenly dispersed in the cosmetic composition and the effect of the present invention is best exhibited.

The present invention also provides a skin elasticity enhancement, skin wrinkle improvement or skin moisturizing method comprising applying a rattan extract to the skin of an individual. The subject includes, without limitation, mammals including rats, livestock, humans, and the like.

According to another embodiment of the invention, the invention is sumac (Rhus Semialata ) as an active ingredient. Specifically, the present invention provides a pharmaceutical composition for moisturizing the skin and / or a pharmaceutical composition for improving skin wrinkles comprising the extract of Rhus verniciflua as an active ingredient. More particularly, the present invention is to provide a pharmaceutical composition for skin moisturization and / or a composition for improving skin wrinkles comprising seed and / or root extract of rhusin. More specifically, the present invention is to provide a pharmaceutical composition for skin moisturization and / or a pharmaceutical composition for improving skin wrinkles, which comprises a seed extract of rhusin.

The composition comprising the compound of the present invention may be various oral or parenteral formulations, but may preferably be a parenteral agent. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, and emulsions. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent.

The present invention can also be provided as a formulation for skin elasticity enhancement, skin wrinkle improvement, or skin external application for skin moisturizing, comprising the extract of rattan as an active ingredient.

In the case of using the rhusin extract as an external preparation for skin, it may further contain at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, foaming agents, , Ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for skin ≪ / RTI > any other ingredients that are commonly used in the skin sciences. The components can also be introduced in amounts commonly used in the field of dermatology.

When the rattan extract is provided as an external preparation for skin, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.

The pharmaceutical composition of the present invention is particularly preferably used as a parenteral preparation. For example, the external preparation for skin may be a pharmaceutically acceptable base such as vaseline or stearyl alcohol; Suitable pharmaceutically acceptable surfactants such as polysorbate, sorbitan sesquioleate, and the like; A suitable pharmaceutically acceptable humectant such as glycerin; A suitable pharmaceutically acceptable solvent; And a conventional external preparation for skin preparation in which a flavoring agent, a coloring agent, a stabilizer, a tackifier and the like are homogeneously mixed.

In addition, when the rattan extract of the present invention is used as a medicine, it may further contain one or more active ingredients showing the same or similar functions. For example, it may contain known components for skin elasticity enhancement, skin wrinkle improvement, or skin moisturizing. The skin elasticity enhancement, skin wrinkle improvement or skin moisturizing effect of the composition of the present invention may be further enhanced if the skin elasticity enhancing agent, the skin wrinkle improving agent or the skin moisturizing agent is included. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use.

The pharmaceutical composition of the present invention can provide skin elasticity enhancement, skin wrinkle improvement, or skin moisturizing effect when the effective amount of the extract of Ruka nigra is contained. In the present invention, the term "effective amount" means the amount of a compound capable of sufficiently exhibiting skin elasticity enhancement, skin wrinkle improvement, or skin moisturizing effect.

The effective amount of the rhusin extract contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, how the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized as a pharmaceutical product, the extract can be contained at a higher concentration than the case where it is commercialized as cosmetics to be applied to skin on a daily basis. Accordingly, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the compound of formula (1) 6 times a day.

The composition containing the rhusin extract of the present invention exhibits excellent effects on skin elasticity enhancement, skin wrinkle improvement or skin moisturization.

The composition containing the rhusin extract of the present invention can be used as a raw material for cosmetics or a pharmaceutical ingredient which is safe for skin and has excellent skin condition improving effect.

Fig. 1 shows the results of confirming the inhibitory effect of hyaluronidase on the part of rhusi.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided by way of example to facilitate a specific understanding of the present invention.

Preparation of rhusi root and seed extract

The extracts of the rusks used in the following examples were obtained from Miyang huaxiang zhongye Co., Ltd., 10 times of ethanol was added and the mixture was heated at 60 ° C for 4 hours.

[ Example  1] Rhus root extract ( Rhus Semialata  Root extract and rhododendron seed extract Rhus Semialata  Seed extract to inhibit hyaluronidase activity

50 mL of hyaluronidase (1 ng / mL) (R & D systems; 7358-GH-020) was placed in a microtube and 50 mL of the evaluation reagent was added and incubated at 25 DEG C for 1 hour. As the evaluation agent, Rhus root extract ( Rhus Semialata Root extract and Rhus seed extract Semialata Seed extract) was used. For comparison, Gallotannin (Santa Cruz Biotechnology; sc-202619) was added to the untreated group and the positive control group. The extracts of rusch roots and rhododendron seeds were added at concentrations of 0.4, 10 and 250 ppm, respectively. Gallotannin was added at 850 ppm. After incubation, the amount of hyaluronic acid degraded by the activity of hyaluronidase at each concentration was measured at 450 nm using a spectrophotometer with hyaluronidase activity ELISA (Hyaluronidase Activity ELISA, Echelon; K-6000) . The results of the activity of the hyaluronidase in the untreated group as 100 are shown in Table 2 and FIG.

sample Hyaluronic acid (ng / ml) Hyaluronidase activity (%) for control Control (untreated group) 0.32 100 Gallotannin 850 ppm 3.96 11.80034 Rhus Semialata Stem extract 250 ppm 1.43 73.10395 Rhus Semialata Leaf extract 250 ppm 0.88 86.43082 Rhus Semialata Root extract 0.4 ppm 1.2 78.67701 Rhus Semialata Root extract 10 ppm 2.433 48.80058 Rhus Semialata Root extract 250 ppm 4.117 7.996123 Rhus Semialata Seed extract 0.4 ppm 1.41 73.58856 Rhus Semialata Seed extract 10 ppm 2.965 35.90986 Rhus Semialata Seed extract 250 ppm 4.332 2.786528

As shown in the above Table 1 and Fig. 1, when the activity of hyaluronidase is inhibited by gallotannin of 90% or more by the positive control group, the extracts of rhusi root and rhodinus seed inhibit the activity of hyaluronidase in a concentration- . The hyaluronidase inhibitory activity of Rhus verniciflua and leaf extracts was significantly lower than that of roots and seed extracts.

[ Example  2] Root extract of rhusi ( Rhus Semialata  Root extract and rhododendron seed extract Rhus Semialata  Seed extract Ella Stays  ( Elastase ) Inhibitory activity

In order to investigate the other functions of rhus roots and seed extracts, 100 mUnit / mL unit elastase and 0.5 mM MeOSuc-Ala-Ala-Pro-Val as a substrate of Elastase were added to 100 mM Tris-HCl buffer p-nitroanalide (Bachem; L-1335) was added and reacted at 25 ° C for 20 minutes. Then, the absorbance was measured at 405 nm to quantitate the amount of p-nitroanilide produced from the substrate. EGCG was used as a comparator.

Rhus Semialata Seed Extract showed an inhibition activity of 87.15% at 2 ppm concentration. Rhus Semialata Root Extract showed an inhibition activity of Elastase at 33.15% at a concentration of 2 ppm. It can be seen that the rhusjian seed extract shows an inhibitory activity against Elaastase at a lower concentration than that of the root extract, which shows activity similar to that of EGCG (Epigallocatechin gallate) showing an inhibition activity of 96.23%. At this time, Rhus Semialata Stem Extract and Rhus Semialata Leaf Extract were 21.09% and 15.28%, respectively, at a concentration of 200 ppm. The inhibitory activity of the Rhus Semialata Stem Extract was significantly lower than that of the root and seed extracts .

sample Elastase activity (%) Elastase activity inhibition rate (%) Control (untreated group) 100 - EGCG 1 ppm 3.77 96.23 Rhus Semialata Stem extract 200 ppm 78.91 21.09 Rhus Semialata Leaf extract 200 ppm 84.72 15.28 Rhus Semialata Seed extract 200 ppm 9.23 90.77 Rhus Semialata Seed extract 100 ppm 8.41 91.59 Rhus Semialata Seed extract 50 ppm 9.64 90.36 Rhus Semialata Seed extract 10 ppm 12.54 87.46 Rhus Semialata Seed extract 2 ppm 12.85 87.15 Rhus Semialata Seed extract 0.4 ppm 88.27 11.73 Rhus Semialata Root extract 200 ppm 4.13 95.87 Rhus Semialata Root extract 100 ppm 4.49 95.51 Rhus Semialata Root extract 50 ppm 7.24 92.76 Rhus Semialata Root extract 10 ppm 31.41 68.59 Rhus Semialata Root extract 2 ppm 66.85 33.15 Rhus Semialata Root extract 0.4 ppm 74.71 25.29

[ Manufacturing example ] Cream manufacturing

As shown in the composition of Table 3 below, creams containing rusch seed extract were prepared by conventional methods. In addition, a cream prepared from the other components except for the active ingredient was prepared in Comparative Example 1.

Composition (% by weight) Production Example 1 Comparative Example 1 Rhododendron seed extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water to 100 to 100

[ Example  3] Confirm skin wrinkle improvement effect through panel test

The cream of Preparation Example 1 and Comparative Example 1 was tested for the effect of improving skin wrinkles in healthy 35 to 50 year old women as follows.

30 women aged 35 to 50 years were divided into two groups by 15 persons. In Group 1, the cream of Example 1 containing 1 wt% of rhus chinensis seed extract, the cream of Comparative Example 1 in Group 2, the cream of Example 1 Once a day for 3 months. After 3 months, improvement of skin wrinkles was evaluated by image analysis of questionnaires and skin wrinkles. The subjects' questionnaires were judged to be three stages of improvement of skin wrinkles and improvement of skin elasticity, no improvement, slight improvement, and significant improvement compared to before use, and the results are shown in Table 4 below.

Category (persons) No improvement Slight improvement A significant improvement Production Example 1 One 7 7 Comparative Example 1 10 4 One

As can be seen from Table 4, when the cream of Preparation Example 1 containing the rhus seed extract was used, it was confirmed that the effect of improving the skin wrinkles was better than that of the cream of Comparative Example 1 which was not used.

Claims (10)

A composition for improving skin wrinkles containing rhus chinense extract as an active ingredient. The composition for improving skin wrinkles according to claim 1, wherein the rhus verniciflua extract is a root extract, a seed extract or a mixture thereof. 2. The composition for improving skin wrinkles according to claim 1, wherein the Rhus verniciflua extract is a seed extract of Rhus verniciflua. [3] The composition for improving skin wrinkles according to claim 1, wherein the rumen extract is contained in an amount of 0.00001 to 10% by weight based on the total weight of the composition. The composition for improving skin wrinkles according to claim 1, wherein the composition for improving skin wrinkles is a cosmetic composition. A composition for moisturizing the skin comprising Rhus javanica extract as an active ingredient. [Claim 7] The composition for skin moisturizing according to claim 6, wherein the Rhus verniciflua extract is Root Extract, Seed Extract or a mixture thereof. 7. The composition for moisturizing the skin according to claim 6, wherein the Rhus verniciflua extract is a seed extract of Rhus verniciflua. [Claim 7] The skin moisturizing composition according to claim 6, wherein the rumen extract is contained in an amount of 0.00001 to 10 wt% based on the total weight of the composition. The skin moisturizing composition according to claim 6, wherein the composition for moisturizing the skin is a cosmetic composition.

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