KR20180082058A - A cosmetic composition for skin desquamation comprising natural complex extract - Google Patents

Abstract

One aspect of the present invention provides a cosmetic composition for keratolysis comprising: betaine salicylate, gluconolactone, lactobionic acid; and skin extracts of Citrus unshiu and Garcinia mangostana as active ingredients. A cosmetic composition according to one aspect of the present invention comprises a natural extract as an active ingredient to minimize skin irritation caused by a chemical agent, and keratolysis and skin moisturizing effects can be remarkably improved.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for skin exfoliation comprising a natural combination extract as an active ingredient,

The present invention relates to a cosmetic composition for exfoliating a skin containing a natural extract.

The stratum corneum is composed of 10 to 20 layers of dead cells and intercellular nerves, natural moisturizing factor (NMF) present in the outermost part of the skin, which protects the skin from external pollutants, Lt; RTI ID = 0.0 > of water < / RTI >

In healthy individuals, the differentiated cells from the basal layer mature and reach the granule layer. On the 14th day, the cells reaching the granule layer disappear and disappear, and it takes 14 days until they become keratinocytes and arrive at and disappear from the stratum corneum. The turn-over period of the skin stratum corneum is about 4 weeks. When a predetermined period elapses, the old stratum corneum is naturally peeled off and a new stratum corneum is formed.

However, due to internal factors such as aging and external factors such as ultraviolet rays, the turnover cycle becomes long and unbalanced, and the stratum corneum is old and stratified, resulting in a rough skin, uneven skin tone, and skin incongruity.

One of the signs of aging skin is a reduction in the ability to dislodge dead cells, which causes the skin to lose vitality and elasticity as the cell regeneration cycle increases. In order to improve this, efforts have been actively made to restore skin elasticity by stimulating cell regeneration of the basal layer by removing the skin layer through a skin peeling technique.

There are three kinds of exfoliating cosmetics. The first is a method of finely cutting the skin by physical action such as skin friction or laser. The second is a chemical method in which an AHA (Alpha-Hydroxy Acid), BHA (Beta- Hydroxy Acid), and urea, and the third is a method of peeling the keratin using a proteolytic enzyme having a function of exfoliation.

In physical removal, skin irritation is severe and it is not suitable for home use. In the case of chemical method, it is not effective because it is possible to use only low volume in household products. Protein peeling agents are expensive and ineffective.

Meanwhile, the cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

Accordingly, the present inventors developed an eco-friendly and highly functional cosmetic product for peeling off the skin, which contains only natural ingredients corresponding to EWG 1 grade and can safely use sensitive skin.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a functional cosmetic composition which is less irritating to the skin and effectively removes keratin.

According to one aspect of the present invention there is provided a composition comprising BETAINE SALICYLATE, GLUCONOLACTONE, LACTOBIONIC ACID; And a tangerine skin extract and a mangosteen skin extract as an active ingredient are provided.

In one embodiment, the betaine salicylate may be included in an amount of 0.1 to 1.0 wt% based on the total weight of the composition.

In one embodiment, the cosmetic composition may further include at least one selected from the group consisting of chestnut shell extract, yellow white extract, and thallus extract.

In one embodiment, the orange outer coat extract and the mangosteen outer coat extract are selected from the group consisting of Bifidobacterium spp . ) Strain of the present invention.

In one embodiment, the cosmetic composition may comprise 10-30 parts by weight of the mandarin shell extract and 10-30 parts by weight of the mangosteen husk extract.

In one embodiment, the extract may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butyleneglycol.

In one embodiment, the cosmetic composition is selected from the group consisting of softening longevity, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, Or more.

The cosmetic composition according to one aspect of the present invention contains a natural extract as an active ingredient, minimizing skin irritation caused by a chemical preparation, and exfoliation and skin moisturizing effect can be remarkably improved.

In particular, the natural raw material applied to the cosmetic composition of the present invention is a raw material corresponding to the EWG 1 grade and can be applied safely to sensitive skin without harmfulness to human body.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention there is provided a composition comprising BETAINE SALICYLATE, GLUCONOLACTONE, LACTOBIONIC ACID; And a cosmetic composition for exfoliating keratin comprising a mandarin skin extract and a mangosteen skin extract as an active ingredient.

The cosmetic composition minimizes the chemical preparation and substitutes the chemical agent with an extract of natural origin, thereby achieving superior skin exfoliation and hypoallergenic properties.

As used herein, the phrase " comprising as an active ingredient " means containing an effective amount capable of exhibiting a skin improving effect, such as collagen synthesis associated with a wrinkle-reducing effect, elasticity improvement or skin soothing, exfoliating effect .

In particular, since the cosmetic composition contains only EWG level 1 components, it satisfies both functionality and safety, and is easy to apply to sensitive skin because it is not harmful.

EWG is a cosmetic ingredient harmful grade announced by EWG (Environmental Working Group), a nonprofit environmental NGO in the United States. EWG carefully examines the harmfulness of cosmetic ingredients and classifies the hazard level from 1 to 10, and 1 Class 2 and Class 2 are classified as safety class.

The term " exfoliating the skin " means that the keratin substances accumulated on the stratum corneum of the skin are removed or removed from the stratum corneum of the skin. The exfoliation can be performed not only by removing physical force but also by applying a cosmetic composition And the case where it is separated from the stratum corneum.

The term " hypoallergenic " means that the irritation of the skin such as redness and stinging of the skin is relatively reduced. The cosmetic composition of the present invention can be understood as a cosmetic composition for removing hypoallergenic skin which maintains exfoliation effect while inducing less redness or stinging of the skin as compared with a cosmetic containing only a high amount of betaine salicylate.

BETAINE SALICYLATE is a raw material for synthesizing salicylic acid and betaine. It is superior in moisturizing effect to beta-hydroxy acid used for normal exfoliation purposes, It is significantly lower.

The gluconolactone is an artificial compound used as a food additive and exhibits a mild sour taste. It has low reactivity at low temperature and uniformly produces bubbles, and is also used as a raw material used in foods to lower the pH. The gluconolactone is effective for photoaging and sensitive skin, increases moisturizing power to provide a conditioning effect as a skin barrier, and is effective in managing wrinkles, skin firings, and pores.

The Lactobionic Acid is a polyhydroxy acid (PHA) derived from Milk Sugar and is a potential antioxidant with excellent water supply ability due to its ability to attract and bind water molecules. The lactobionic acid component reduces the wrinkles of the skin, makes the skin tissue firm, reduces the aging phenomenon, inhibits the function of the destructive enzyme (MMP) which degrades the function of maintaining the texture and strength of the skin, To protect against damage from sunlight.

At this time, the betaine salicylate may be contained in an amount of 0.1 to 1.0 wt% based on the total weight of the composition.

In order to minimize the irritation and the deterioration of the exfoliating effect, the cosmetic composition has a reduced content of betaine salicylate and a small amount of the gluconolactone and lactobionic acid, thereby realizing functional and hypoallergenic properties . However, if the content of betaine salicylate is less than 0.1% by weight, the exfoliating effect may not be sufficiently achieved, and if it is more than 1.0% by weight, irritation to the skin may increase.

Meanwhile, the cosmetic composition may contain, as an active ingredient, a mandarin skin extract and a mangosteen skin extract.

Citrus unshiu Marcov . ) "Is rich in vitamin C and citric acid, and contains vitamin P, phosphorus, and hesperidin, which are excellent for strengthening capillary blood vessels. The active ingredient is contained in the outer skin more than the flesh, especially about 4 times as much as the vitamin C. The tangerine skin extract has an excellent effect of neutralizing IL-8, which is an inflammatory substance that stimulates melanocyte production by stimulating melanocyte, and can relieve irritation caused by exfoliation and provide moisture to the skin.

The " Garninia " mangostana L. ) "is mainly produced in Malaysia. The tree is about 10 m high and 1 m in diameter. Leaves are opposite, long oval, thick, with flat edges. The flowers are bloomed in April and April in origin, and the male flower is on the end of the branch. The female flower is cut into 1 ~ 2 pieces on the leaf axil, and it is light pink.

The genital mangosteen fruit and skin are known to have excellent antioxidant ability because it contains a typical antioxidant component called xanthone, which is a type of polyphenol. In Southeast Asia, it has been used for a long time as a civilian medicine for inflammation and wound healing . In addition to anti-inflammatory effects, mangosteen is known for its antibacterial and anticancer effects.

The cosmetic composition minimizes the content of betaine salicylate, and at the same time, the exfoliation effect is improved and the irritation is minimized by adding the citrus extract and the mangosteen shell extract.

The above-mentioned "extract" refers to a solvent in which an effective ingredient contained in an extraction raw material has been transferred by contacting a solvent and an extraction raw material under specific conditions. If a substance is separated from a natural product, the extraction method, You can include them all regardless. For example, extracting a component dissolved in a solvent from a natural product using water or an organic solvent, and extracting a specific component of a natural product, for example, extracting only a specific component such as oil.

The extract may be extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, % Concentration (v / v) of ethanol.

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

Particularly, the polarity of water and ethanol is different, so that the effective ingredient to be extracted according to each polarity can be changed, and the concentration of ethanol can be appropriately controlled so that an optimal skin improving effect can be realized. At this time, if the concentration of the ethanol exceeds 90%, an adequate yield may not be realized. If the concentration is less than 60%, the effective ingredient showing skin improving effect may not be extracted sufficiently.

The extract is washed with water, dried and pulverized, and extracted and extracted with a solvent having a volume of 8 to 12 times the weight of the raw material for about 1 to 24 hours by a conventional method such as circulation extraction, pressure extraction, Followed by filtration. In addition, the extract can be obtained in powder form by an additional process such as vacuum distillation or freeze-drying.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

Meanwhile, the cosmetic composition may further comprise at least one selected from the group consisting of chestnut husk extract, Huang Baek extract, and Thunb extract.

The " night " is a chestnut ( Castanea crenata S. ) is a fruit of 100-1100m above sea level in Korea. At night, the flesh and coat contain evenly distributed protein, fat, vitamins and minerals. Vitamin B1 is rich in four times the amount of rice, vitamin C and vitamin D.

The "yellowish (Phellodendron amurensis ) "is a term used to refer to the skin of an Asahi tree as a medicinal herb, also known as the" It is distributed all over the country except Jeju and Chonnam, and it is distributed in Japan, Manchuria, China, Amur, and Sochi, and it lives in forests or mountain valleys. It is known to peel off the husks from the yellowish white stem and to remove the rough skin and dry it in the sun, then taking the medicine and lowering the blood sugar.

The " Hisbiscior cortex " refers to the dried root bark or stem bark of Hibiscus syriacus L., the glans bush belonging to the Mugwort family. The taste is sweet and it is known to work in liver, liver, colon, small intestine and small intestine. It is known that the hairy root contains substances such as Isoeugenol, 2-phenylethanol, acetylbenzaldehyde, indole, lutein, etc., and it is known that it is effective to supplement the exfoliation effect and alleviate the skin irritation caused by the exfoliant .

The chest extract, Huang Baek extract, and Thunb extract may exhibit an excellent exfoliation effect and skin irritation mitigation effect due to interactions between active ingredients derived from each raw material. In addition, the various components contained in each of the above extracts can be applied to the skin to increase the absorption rate.

Since the extract has exfoliation effect, skin moisturizing effect and softening effect, it is possible to diversify the functionality of the cosmetic composition and maximize the exfoliation effect by the betaine salicylate.

The orange outer coat extract and the mangosteen outer coat extract may be a fermentation extract obtained by inoculating a strain of Bifidobacterium spp .

The term " fermentation " means a process of decomposing an organic substance using an enzyme contained in the microorganism. It has been reported that a raw material that has undergone fermentation by the above-mentioned microorganisms has an effect of at least 2 times to a maximum of several tens of times greater than that before fermentation. Fermented metabolites contain various amino acids, organic acids and antioxidants that are good for the skin, promoting skin metabolism and making skin texture resilient and smooth. In addition, as the fermentation process proceeds, the particles become smaller and the toxicity such as heavy metals is decomposed, so that the absorption rate is good, and skin troubles and allergic side effects can be alleviated.

The Bifidobacterium spp . ) Is a microorganism which mainly utilizes oligosaccharides containing aminosugars and promotes the digestion and absorption of proteins by inhibiting the development of harmful bacteria in the intestines and provides a vitamin group to the body. The Bifidus fermented extract has a natural moisturizing factor NMF, a precursor of natural moisturizing factor, promotes the conversion of natural moisturizing factor to moisturizing skin and imparting moisture and shine to the skin. In addition, the polysaccharide component rich in the fermented extract can inhibit water evaporation in the skin surface.

The bifidus strain may be Bifidobacterium breve), Bifidobacterium Bifidobacterium bonus (Bifidobacterium may be bifidum), Bifidobacterium ronggeom (Bifidobacterium longum), or Bifidobacterium inpeon tooth (Bifidobacterium infantis) may be a, preferably Bifidobacterium bipyridinium bushes (Bifidobacterium bifidum).

The above-mentioned " fermentation extract " is obtained by drying the extract naturally or by using an apparatus, specifically a rotary vacuum concentrator and a freeze drier, diluting the pomegranate extract to a certain concentration in a solvent, preferably purified water, , That is, by inoculating and fermenting a bifidus strain.

The cosmetic composition may include 10-30 parts by weight of the orange outer coat extract and 10-30 parts by weight of the mangosteen outer shell extract. In the case where the content deviation between the respective active ingredients constituting the extract is out of the above range, a synergistic effect due to interaction between the active ingredients may not be properly realized. Therefore, considering the working environment and the quality of the final product, Can be appropriately controlled.

The extract may be extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol.

The cosmetic composition may be formulated into one or more selected from the group consisting of softening agents, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, have.

The cosmetic composition may be formulated by a conventional method in the art. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; A foundation such as a liquid type, a solid type or a spray type; powder; Make-up removers such as cleansing creams, cleansing lotions and cleansing oils; Or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. The external preparation for skin may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the cosmetic composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example  1: Preparation of extract

After washing the mandarin shell, mangosteen shell, chestnut shell, yellowish white, and thorny root, they were thoroughly dried at room temperature and pulverized to obtain 100 g of pulverized products.

100 g of each pulverized product was immersed and extracted with a 10-fold volume of an aqueous ethanol solution (70% mass concentration) as a solvent at a temperature of 70 ° C for 12 hours.

The extract was filtered with 250 mesh and 0.5 mu m filters and then extracted three times in the same manner for the remaining raw materials. The extract was concentrated under reduced pressure at 30 DEG C and lyophilized to obtain a solid fraction.

Manufacturing example  2: Preparation of fermented extract

The fermentation medium (glucose 1.5% (v / v), sugar 1.5% (v / v), starch syrup 1.5% (v / v), yeast extract 0.3%) containing glucose, sugar, syrup and yeast extract v / v)) using Bifidobacterium Bifidobacterium bonus (Bifidobacterium bifidum ) was prepared.

10 mg of Bifidobacterium bifidum dried material was pre-cultured in the above medium (10 mL). The mangosteen extract and the mangosteen husk extract of Preparation Example 1 were added to a medium having the same composition as that of the fermentation medium, and the preculture was inoculated and fermented.

Bifidobacterium bifidum was cultivated at 36.5 ℃ for 36 hours and glucose and sugar content were controlled to increase the growth rate of the strain. After the fermentation was completed, the extracts were subjected to centrifugation and filtration to obtain a fermented orange peel extract and a mangosteen peel fermented extract.

Example  And Comparative Example

To examine the skin improving effect of the obtained extract and the fermented extract, examples and comparative examples were set by mixing samples as shown in Table 1 below.

Betaine salicylate, gluconolactone and lactobionic acid were obtained commercially and used.

Each raw material was mixed at a predetermined ratio to measure skin irritation and exfoliation effect and the fermented extract of Preparation Example 2 was mixed in Examples 2 and 4.

[Content (parts by weight)] division Example
One Example
2 Example
3 Example
4 Comparative Example
One Comparative Example
2 Comparative Example
3 Comparative Example
4 Betaine salicylate 80 80 50 50 400 200 200 200 Glucono
Lactone 80 80 50 50 - 100 - - Lacto
Bionic acid 80 80 50 50 - 100 - - Tangerine skin extract
(Production Example 1) 80 - 50 - - - 100 - Mangosteen
Crust extract
(Production Example 1) 80 - 50 - - - 100 - Tangerine skin extract
(Production Example 2) - 80 - 50 - - - - Mangosteen
Crust extract
(Production Example 2) - 80 - 50 - - - - Chestnut extract - - 50 50 - - - 70 Yellowish white
extract - - 50 50 - - - 70 Neck muscle extract - - 50 50 - - - 70

Experimental Example  1: Skin safety test

MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) to verify skin safety for the samples of the Examples and Comparative Examples.

The samples of Examples and Comparative Examples were each suspended in purified water at a concentration (50 mg / L), and cell viability was measured.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

(HDF), melanocyte-forming cells (B16F10), and keratinocytes (HaCaT) were dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 48 hours Respectively.

After the culture medium was removed and the sample was cultured for 48 hours in a concentration-treated medium, the medium was removed and repeatedly washed with PBS (phosphate buffered saline). MTT was dissolved in PBS at 5 mg / mL and 50 L was added. The cells were cultured at 37 ° C and 5% CO ₂ for 24 hours. 100 L of DMSO (dimethyl sulfoxide) was added to each well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of measurement, cytotoxicity was not confirmed at all concentrations of each sample. The above results suggest that the sample of the composition is not only harmless to human body but also has excellent human safety.

Experimental Example  2: Promoting effect of keratinocyte differentiation

Human keratinocytes were cultured in a culture flask and grown to about 80%. The samples of the examples and comparative examples were added at the concentrations of 200 ppm and treated for 4 days as shown in Table 2 below.

SDS (sodium dodecyl sulfate) as a denaturant and β-mercaptoethanol as a reducing agent were mixed to remove dissolved proteins.

The concentration of keratinocytes was measured by measuring the absorbance of the remaining stratum corneum at 310 ㎚. The amount of keratinocyte was compared with the negative control (0%). At this time, TGF-beta (10 nM), a cell signaling substance, was treated as a positive control.

division Promoting effect of keratinocyte differentiation (%) Example 1 121.5 Example 2 134.2 Example 3 145.3 Example 4 151.6 Comparative Example 1 10.2 Comparative Example 2 17.4 Comparative Example 3 75.4 Comparative Example 4 54.3 Positive control group 143.2

Referring to Table 2, the samples of Examples 1 to 4 were excellent in promoting keratinocyte differentiation, and Bifidobacterium bifidum ) fermentation extracts of Examples 2 and 4 was markedly improved.

In particular, the sample of Example 4 exhibited remarkably improved keratinocyte differentiation promoting effect as compared with TGF-beta, a cell signaling substance.

Experimental Example  3: skin moisturizing effect test

The skin moisturizing power improving effect of the cosmetic compositions containing the samples of Examples and Comparative Examples was evaluated. A cream form cosmetic composition of the same composition was prepared using the samples of Examples and Comparative Examples. Each sample was formulated into cream of the same composition, except that the samples of Examples 1 to 4 and Comparative Examples 1 to 4 were different.

In order to measure the skin elasticity improving effect of the cream form cosmetic composition, 80 subjects (20 to 40 years old women) were divided into 8 groups of 10 persons, and samples of Examples and Comparative Examples were included in the whole face for each group Was applied twice a day for 1 month continuously.

The skin conductivity was measured in advance using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application and the values were measured at 1 week, 2 weeks, The skin conductivity increase rate (%) was measured and evaluated. The results are shown in Table 3 below.

division 1 week 2 weeks 4 weeks Example 1 37 43 58 Example 2 43 48 61 Example 3 47 52 64 Example 4 51 56 71 Comparative Example 1 9 13 16 Comparative Example 2 13 17 21 Comparative Example 3 14 20 24 Comparative Example 4 13 19 25

Referring to Table 3, the experimental group to which the cosmetic composition of Examples 1 to 4 was applied exhibited an excellent skin conductivity increase rate as compared with Comparative Examples 1 to 4. In particular, the cosmetic compositions of Examples 2 and 4 containing the Bifidobacterium bifidum fermented extract had an excellent effect of increasing the skin conductivity.

From the above results, it was confirmed that the cosmetic compositions containing the samples of Examples 1 to 4 kept the skin moisture content higher than the cosmetic compositions containing the samples of the comparative examples, because the skin conductivity was in proportion to the skin moisture content.

Experimental Example  4: Skin elasticity improvement effect test

A cream form cosmetic composition of the same composition was prepared using the samples of Examples and Comparative Examples. Each of the above samples was formulated into cream of the same composition, except that the samples of Examples 1 to 4 and Comparative Examples 1 to 4 were different.

In order to measure the skin elasticity improving effect of the cream form cosmetic composition, 80 subjects (20 to 40 years old women) were divided into eight groups each consisting of 10 persons, and cream containing a sample of each group and a comparative sample was divided into eight groups For 2 consecutive months.

In order to confirm the improvement of skin elasticity, skin elasticity was measured before use and 2 months after using the skin elasticity meter (SEM 575, C + K Electronic Co., Germany). The experimental results are reported as the R7 value of Cutometer SEM 575, and the average value of each group is shown in Table 4 below. The R7 value indicates the nature of the viscoelasticity of the skin (n = 10, p < 0.05).

division R7 Example 1 0.34 Example 2 0.39 Example 3 0.42 Example 4 0.49 Comparative Example 1 0.11 Comparative Example 2 0.16 Comparative Example 3 0.23 Comparative Example 4 0.19

Referring to Table 4, the samples of Examples 1 to 4 effectively improved skin elasticity in the facial area.

In particular, Bifidobacterium Bifidobacterium bonus (Bifidobacterium bifidum ) fermented extract, chestnut husk extract, yellowish white extract, and mokushi extract were remarkably enhanced in the treatment of the cosmetic composition of Example 4.

Experimental Example  5: Evaluation of skin irritation

A skin patch test was conducted to examine the skin irritation test for each of the cream form cosmetic compositions. The subjects were selected as persons without skin disease at the test site of 15 to 30 years old.

80 subjects were divided into 8 groups of 20 persons each. The upper part of the pellet was wiped with 70% ethanol and dried. Then, 0.1 g of the product was applied for 24 hours, the skin reaction was checked within 1 hour after removal of the patch, and the next day (48 hours later). Skin response determinations were made according to the following test criteria. The skin patch test results are shown in Table 5.

division Judgment result - ± + ++ +++ Example 1 15 One One 2 One Example 2 16 2 One - One Example 3 17 One One One - Example 4 19 - One - - Comparative Example 1 4 5 4 4 3 Comparative Example 2 4 5 7 3 One Comparative Example 3 6 6 5 One 2 Comparative Example 4 7 5 4 3 One

* Skin reaction test criteria / -: No reaction / ±: weak positive (erythema) / +: positive (erythema) / ++: strong positive (erythema, edema) / +++: severe positive , Swelling, blistering)

Referring to Table 5, the cream type cosmetics formulated with the samples of Examples 1 to 4 hardly induced skin irritation as compared with Examples 1 to 4.

Experimental Example  6: Sensuality  evaluation

The cream form cosmetic composition was subjected to sensory evaluation.

A total of 80 people, aged between 20 and 40, were applied to each of the age groups. The cream was applied to the right and left hands, and then the cream was applied to each of the hands to massage the gel. The gel cohesion, skin irritation, .

 The highest score is 10 points, the average score is 5 points, and the score is 0 points.

The results of sensory evaluation according to the above Test Example are shown in Table 6 below.

Evaluation items Gel cohesiveness Degree of exfoliation Skin irritation Skin moisturizing power Overall likelihood Example 1 8 7 8 7 7 Example 2 8 8 9 8 8 Example 3 9 8 8 9 8 Example 4 9 9 8 9 9 Comparative Example 1 4 5 4 5 5 Comparative Example 2 5 5 5 6 5 Comparative Example 3 4 6 6 5 6 Comparative Example 4 4 5 6 5 5

Referring to Table 6, it was evaluated that the cream type cosmetics formulated in the samples of Examples 1 to 4 were significantly superior in overall taste as compared with Examples 1 to 4.

In particular, all of the evaluated items, such as gel cohesion, exfoliation effect, skin irritation, and moisturizing ability, were evaluated to be high.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (7)

BETAINE SALICYLATE, GLUCONOLACTONE, LACTOBIONIC ACID; And
A cosmetic composition for exfoliating keratin comprising a mandarin skin extract and a mangosteen skin extract as an active ingredient. The method according to claim 1,
Wherein the BETAINE SALICYLATE is contained in an amount of 0.1 to 1.0 wt% based on the total weight of the composition. The method according to claim 1,
Wherein the cosmetic composition further comprises at least one selected from the group consisting of chestnut extract, chestnut husk extract, and Thunb extract. The method according to claim 1,
The cosmetic composition according to claim 1, wherein the extract of Mandarin shell and Mangosteen shell extract is a fermented extract obtained by inoculating a strain of Bifidobacterium spp . The method according to claim 1,
10 to 30 parts by weight of the mandarin outer skin extract and 10 to 30 parts by weight of the mangosteen outer shell extract. The method according to claim 1,
Wherein the extract is extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. 7. The method according to any one of claims 1 to 6,
A cosmetic composition formulated with at least one selected from the group consisting of softening lotion, astringent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder.