KR20200022646A - Cosmetic composition comprising lactic acid and gluconolactone for enhancing skin barrier - Google Patents

Abstract

The present invention relates to a cosmetic composition for strengthening a skin barrier containing lactic acid and gluconolactone. According to the present invention, the lactic acid and gluconolactone strengthen a skin barrier function as it is confirmed that lactic acid and gluconolactone can promote the expression of skin barrier genes. Further, lactic acid and gluconolactone can also prevent skin dryness or skin inflammation caused by damage to the skin barrier, thereby being useful in the field of cosmetics and food.

Description

Cosmetic composition for enhancing skin barrier including lactic acid and gluconolactone

The present invention relates to a cosmetic composition for skin barrier strengthening comprising lactic acid and gluconolactone.

Skin plays a very important role as a barrier to protect individuals from the outside. Barrier function is to protect against various stimuli (chemicals, air pollutants, dry environment, ultraviolet rays, etc.) from the outside and to prevent excessive release of moisture through the skin, and this protection function is a stratum corneum composed of keratinocytes. The function can be maintained only when is normally formed.

The outermost stratum corneum (horney layer) of the epidermis is formed from keratinocytes and consists of complete differentiated keratinocytes and the surrounding lipid layer (Marcelo CL et al, J. Invest. Dermatol., 80, pp 37-44, 1983). Keratinocytes are characteristic cells whose basal cells, which continuously proliferate in the epidermal layer, undergo morphological and functional changes in stages and rise to the surface of the skin. Exfoliated and new keratinocytes take over their function. This repetitive process of change is called epidermal differentiation or keratinization. In addition, during keratinization, keratinocytes form a natural moisturizing factor (NMF) and intercellular fats (ceramides, cholesterol, fatty acids), forming a stratum corneum, which makes the stratum corneum firm and flexible, thus creating a skin barrier. function as a barrier.

The stratum corneum can easily lose its function due to excessive face washing, lifestyle factors such as bathing, environmental factors such as dry air pollutants, and endogenous diseases such as atopic or aged skin. Recently, due to a variety of factors, more and more dry skin symptoms and related complaints are increasing in recent years. In addition, various changes in the skin may occur as the external environment changes and the aging progresses, resulting in damage to the skin barrier, and thus skin inflammation, dryness, and itching may occur.

Therefore, it is possible to improve various problems occurring on the skin by improving the skin barrier. Recently, studies on this have been actively conducted, but there are not many reports of raw materials to improve the skin barrier.

The object of the present invention is to provide a lactic acid or an acceptable salt thereof; And gluconolactone or an acceptable salt thereof.

Another object of the present invention is to provide a lactic acid or an acceptable salt thereof; And it provides a cosmetic composition for inducing skin keratinocyte differentiation comprising gluconolactone or an acceptable salt thereof.

Another object of the present invention is to provide a lactic acid or an acceptable salt thereof; And gluconolactone or an acceptable salt thereof to provide a health food composition for skin barrier strengthening.

Another object of the present invention is to provide a lactic acid or an acceptable salt thereof; And gluconolactone or an acceptable salt thereof to provide a health functional food composition for skin barrier strengthening.

In order to achieve the above object,

The present invention relates to lactic acid or an acceptable salt thereof; And it provides a cosmetic composition for skin barrier strengthening comprising gluconolactone or an acceptable salt thereof.

In addition, the present invention relates to lactic acid or an acceptable salt thereof; And it provides a cosmetic composition for inducing skin keratinocyte differentiation comprising gluconolactone or an acceptable salt thereof.

The present invention furthermore provides a lactic acid or an acceptable salt thereof; And gluconolactone or an acceptable salt thereof provides a health food composition for skin barrier strengthening.

In addition, the present invention relates to lactic acid or an acceptable salt thereof; And gluconolactone or an acceptable salt thereof provides a health functional food composition for skin barrier strengthening.

Lactobacillus and gluconolactone according to the present invention have been shown to increase the expression of the skin barrier gene when treated on keratinocytes, thereby enhancing the skin barrier function and further preventing skin dryness or skin inflammation caused by skin barrier damage. Therefore, it can be usefully used in cosmetics and food.

Figure 1 shows the results of measuring cytotoxicity in HaCaT cells in the mixed treatment of lactic and gluconolactone.
Figure 2 is a result confirming the increase in the expression level of the skin barrier gene by a single or mixed treatment of lactic acid and glucono lactone.

Hereinafter, the present invention will be described in detail.

Cosmetic composition

The present invention relates to lactic acid or an acceptable salt thereof; And it provides a cosmetic composition for skin barrier enhancement or skin keratinocyte differentiation induction comprising gluconolactone or an acceptable salt thereof.

In the cosmetic composition according to the invention, the cosmetic composition is the lactic acid or an acceptable salt thereof; And gluconolactone or an acceptable salt thereof in an amount of 0.0001 to 90 parts by weight based on the total weight of the composition, but is not limited thereto, including an effective amount that can provide a desired effect.

The cosmetic composition according to the present invention is an external skin ointment, cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, It consists of lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser It may have a formulation selected from, but is not limited thereto. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the type and amount of these components can be readily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream or gel, animal carriers, plant fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Formulations of the present invention may further contain excipients, including fluorescent substances, fungicides, hydrogenic agents, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like. .

When the cosmetic composition is formulated as a medicine or cosmetic as described above, it may include a known excipient applicable to the skin to act as a carrier for the active ingredient. When formulated as a drug, reference may be made to the contents disclosed in [Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA], and when formulated as a cosmetic, [International cosmetic ingredient dictionary, 6th ed., The cosmetic, Toiletry] and Fragrance Association, Inc., Washington, 1995]. The documents are included as part of this specification.

Health food or dietary supplement composition

The present invention relates to lactic acid or an acceptable salt thereof; And gluconolactone or an acceptable salt thereof provides a health food or health functional composition for skin barrier strengthening.

Lactic acid or acceptable salts thereof of the present invention; And when using gluconolactone or an acceptable salt thereof as a nutraceutical and health food composition, there is no particular limitation on the type of food. Examples of foods to which the lactic acid and gluconolactone water of the present invention can be added include drink, meat, sausage, bread, biscuit, rice cake, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, and ice cream. Dairy products, various soups, beverages, alcoholic beverages and vitamin complexes, dairy products and dairy products, and the like, and includes all of the health functional foods and health food compositions in the conventional sense.

Lactic acid or acceptable salts thereof according to the invention; And the nutraceutical and health food composition containing gluconolactone or an acceptable salt thereof may be added to the food as it is or used with other food or food ingredients, and may be appropriately used according to conventional methods. Lactic acid or acceptable salts thereof; And the mixing amount of gluconolactone or an acceptable salt thereof may be suitably determined depending on the purpose of use (prevention or improvement). In general, the amount of the composition in the nutraceutical and health food composition may be added to 0.1 to 90 parts by weight of the total food weight. However, in the case of prolonged ingestion for health maintenance or for health control, the amount may be below the above range, and there is no problem in terms of safety, so there is no problem in lactic acid or an acceptable salt thereof; And gluconolactone or an acceptable salt thereof may be used in an amount above the above range.

The nutraceuticals and health food compositions of the present invention may be used as essential ingredients in the indicated proportions of the present invention or its acceptable salts; And other ingredients other than those containing gluconolactone or an acceptable salt thereof, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 nutraceutical and health food composition of the present invention.

In addition to the lactic acid of the present invention or an acceptable salt thereof; And dietary supplements and health food compositions containing gluconolactone or acceptable salts thereof include flavors, colorants and neutralizing agents such as various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, such as cheeses, Chocolate, etc.), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the nutraceutical and health food composition of the present invention may contain the flesh for the production of natural fruit juice and fruit juice drinks and vegetable drinks.

These components can be used independently or in combination. The proportion of such additives is not so critical, but the lactic acid of the present invention or acceptable salts thereof; And 0.1 to about 20 parts by weight per 100 parts by weight of the nutraceutical and health food composition containing gluconolactone or an acceptable salt thereof.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are merely to illustrate the present invention, the contents of the present invention is not limited by the following examples.

Experimental Example 1 Cytotoxicity Measurement

HaCaT cells, a type of human skin / keratocyte, were cultured in a medium containing DMEM (Dulbecco'Modified Eagle '), 10% FBS (Fetal Bovine Serum) and 1% Penicillin / Streptomycin and sustained 5% CO ₂ Were maintained in a 37 ^° C. incubator. The cells were cultured on 96-well plates and treated with lactic acid and gluconolactone for 48 hours when grown more than 90% (90% of Visual Confluency). Thereafter, the culture medium was removed, and the cells on the 96-well plate were washed three times with PBS (Phosphate-buffered saline). Next, after adding 10 μL of CCK-8 solution (CK04, Dojindo Molecular Technologies, USA), the reaction was performed at 37 ^° C. for 4 hours, and then the absorbance at OD = 450 nm was measured to confirm cell viability.

As a result, as shown in Figure 1, it was confirmed that the treatment of lactic acid and gluconolactone of 0.1% or less has little effect on the survival of keratinocytes, a kind of human skin cells.

Experimental Example 2 Effect of Lactic Acid and Gluconolactone on Skin Barrier Function

HaCaT cells, a type of human skin / keratocyte, were cultured in medium containing DMEM (Dulbecco'Modified Eagle ') and maintained in a 37 ^° C. incubator with a continuous supply of 5% CO2. Lactic Acid and Gluconolactone were treated at 90% of Visual Confluency when grown above well plate, and the concentration was 0.1% for 48 hours. After removing the culture medium, the cells on the plate were washed three times with PBS (Phosphate-buffered saline), purified RNA from the collected cells, and cDNA was synthesized, and gene quantification was performed using qPCR. .

Real-time polymerase chain reaction (real-time PCR, quantitative real time polymerase chain reaction, qPCR) is an experimental method based on polymerase chain reaction. Amplification and positive measurement can be done simultaneously. For one or more specific sequences in a DNA sample, real-time polymerase chain reaction allows detection and quantitation, and metering can count absolute copies or relative amounts.

The experiment proceeded according to the general polymerase chain reaction. An important feature is that the amplified DNA is measured in real time, which is different from the basic polymerase chain reaction where DNA is observed at the end. Two general methods were used in real time polymerase chain reaction. The reporter was labeled with an unspecific fluorescence stain that could interrupt any DNA and a sequence-specific DNA probe-fluorescence consisting of oligonucleotides and was detected after binding to complementary DNA targets. The technique used is the detection of non-specific double helix using SYBR reagent.

IVL (Involucrin) gene is a protein component of human skin, and binds to Loricrin protein to form the membrane of corneocytes that make up the skin barrier. Involucrin is a highly reactive, well soluble, and representative protein used as a substrate for transglutamases. It is present in keratinocytes and other keratinized epidermis of the epidermis, first observed in the cytoplasm but later transformed into membrane protein by Transglutamase. It is mainly synthesized in the stratum spinosum and plays a structural role in the skin barrier. It is an essential protein for the skin to maintain homeostasis from external microorganisms and various irritants. Involucrin, in particular, plays an important role in the skin's inflammatory response and pruritus.

EVPL (Envoplakin) gene is a type of plakin gene that produces proteins that make up the membrane of the epidermal keratin and the desmosome, which is a key structure for the junction between keratinocytes, and is a key protein in the keratin of human skin.

As a result of lactic acid (LA), gluconolactone (GL), and lactic acid and gluconolactone mixed treatment (LA + GL) on HaCaT cells, a kind of human skin cells / keratocyte, as compared to the single treatment as shown in FIG. It was confirmed to increase the expression level of the gene.

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown not in the foregoing description but in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (10)

Lactic acid or acceptable salts thereof; And
Cosmetic composition for skin barrier strengthening comprising gluconolactone or an acceptable salt thereof.
The method of claim 1,
The composition is characterized in that to increase the expression of involucrin or envoplakin (envoplakin).
The method of claim 1,
The composition is characterized in that to prevent skin dryness or skin inflammation.
The method of claim 1,
The lactic acid or acceptable salts thereof; And the content of gluconolactone or an acceptable salt thereof is 0.001 to 50% by weight, based on the total weight of the composition.
The method of claim 1,
The composition is an external skin ointment, cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion , Moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation, nourishing essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser A composition characterized in that it is prepared in one formulation.
Lactic acid or acceptable salts thereof; And
Cosmetic composition for inducing skin keratinocyte differentiation comprising gluconolactone or an acceptable salt thereof.
Lactic acid or acceptable salts thereof; And
Health food composition for skin barrier strengthening comprising gluconolactone or an acceptable salt thereof.
The method of claim 7, wherein
The health food is a health food composition, characterized in that selected from a variety of drinks, meat, sausages, bread, candy, snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gum, tea and vitamin complexes.
Lactic acid or acceptable salts thereof; And
Health functional food composition for skin barrier strengthening comprising gluconolactone or an acceptable salt thereof.
The method of claim 9,
The health functional food is a health functional food composition, characterized in that the food in the form of tablets, capsules, pills or liquids.

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