KR102142461B1 - Composition for improving skin condition comprising extract of korean fir - Google Patents

Abstract

The present specification relates to a composition comprising a bulbous tree extract, and more particularly, to a composition showing a skin moisturizing effect or atopic dermatitis improvement effect, acne improvement effect, and skin inflammation improvement effect by including the bulbous tree extract. Therefore, the composition comprising the extract of the bulbous tree disclosed in the present specification may be usefully used as a pharmaceutical, food, or cosmetic composition, and in particular, may be used as an external preparation for skin to exhibit its effect.

Description

A composition for improving skin condition, including the extract of locust tree {COMPOSITION FOR IMPROVING SKIN CONDITION COMPRISING EXTRACT OF KOREAN FIR}

The present specification relates to a composition comprising a bulbous tree extract.

The most important function of the epidermis, located at the outermost part of the skin, is to protect against various external stimuli (chemical substances, air pollutants, dry environment, physical and chemical irritants such as ultraviolet rays) and to protect the body's moisture through the skin. It is a protective function to prevent excessive divergence, and this protective function can be maintained only when the stratum corneum composed of keratinocytes is normally formed. Among the epidermis, the outermost stratum corneum (horney layer) is formed from keratinocytes, and consists of keratinocytes with complete differentiation and a lipid layer surrounding them (J. Invest. Dermatol. 1983;80:44~ 49). Keratinocytes are characteristic cells in which basal cells, which continuously proliferate in the lowermost layer of the epidermis, undergo changes in shape and function step by step, and rise to the skin surface, and after a certain period of time, old keratinocytes New keratinocytes are eliminated and take over their function, and this repetitive sequence of changes is called'epidermal cell differentiation' or'keratinization'. During the keratinization process, the keratinocytes form a stratum corneum while producing natural moisturizing factor (NMF) and intercellular lipids (ceramide, cholesterol, fatty acid), making the stratum corneum firm and flexible, thereby creating a skin barrier. ) To retain the function.

However, such a stratum corneum may easily lose its function due to excessive washing, lifestyle factors such as bathing, environmental factors such as dry air, pollutants, and endogenous diseases such as atopic skin or senile skin. Due to the increased number of factors in the modern era, the number of people complaining of dry skin symptoms and various disorders caused by this is increasing. Therefore, in order to maintain proper skin moisture, many studies have been conducted to supply moisture from the outside or to prevent moisture loss from the body.In fact, various types of moisturizers with moisture retention ability have been developed, mainly in cosmetics. It is widely used in the field.

However, the formation-turnover rate of the stratum corneum is slowed, the lipid synthesis ability of keratinocytes is lowered, or the division, maturation, and differentiation of normal cells in the epidermis are not smooth. There is a trend of increasing the number of cases in which the amount of skin is reduced so that the normal stratum corneum function cannot be maintained, that is, the skin barrier function is broken.

Atopic dermatitis, which appears due to the breakdown of the skin barrier function, is a chronic recurring skin disease, often accompanied by itching. The etiology of atopic dermatitis is not yet clear, but it is caused by an immunological abnormality mediated by type 2 T auxiliary lymphocytes, and is known to be caused by environmental allergens, genetic predisposition, psychological factors, or pharmacological factors.

Psoriasis is a chronic inflammatory skin disease in which various sized red papules or plaque rashes occur repeatedly on the skin of the whole body. The cause is not yet fully identified, but the activity of immune cells, T cells, increases, causing immune substances to skin. It is believed to cause excessive proliferation and inflammation by stimulating keratinocytes.

In recent years, it has been discovered that abnormally activated immune responses such as hypersensitivity reactions due to excessive T lymphocyte activity and excessive secretion of IgE are one of the main causes of atopic dermatitis, contact dermatitis, and psoriasis disease. Approaches to treating these skin diseases by suppressing the reaction are attracting attention.

Due to the division and differentiation of these abnormal epidermal cells, the skin causes various skin diseases such as dry skin, atopy, and psoriasis, and these diseases can slightly alleviate the symptoms only with conventional moisturizers that have only moisture-retaining function. However, it is difficult to expect fundamental healing.

Acne, one of the representative skin inflammatory diseases, refers to an inflammatory disease of the fur branch, hair follicles and surrounding tissues caused by the action of hormones, excessive sebum secretion, and bacterial infection. There are many causes of acne, but the main causes include excessive secretion of sebum and infection of pores caused by excessive secretion of sebum. On the surface of the skin where the pH is increased due to excessive sebum, the acne causative bacteria easily proliferate, and the proliferated acne bacteria cause purulent inflammation through the weakened skin.

Treatment of acne includes topical formulation therapy and oral medication therapy, and surgical therapy such as laser surgery and dermabrasion that have been recently implemented. Topical application therapy uses antibacterial agents such as azelaic acid and salicylic acid for the purpose of antibacterial action against propionibacterium acnes, which is known as the causative agent of acne, or benzoyl peroxide for the dissolving action of comedones. peroxide) as an external ointment type. However, it is difficult to commercialize it due to formulation and cosmetic raw material laws. Oral medication therapy uses retinoids and corticosteroids for the purpose of suppressing the function of the sebaceous glands and the destruction of the hair follicle wall.These drugs have side effects such as skin rash and dry skin when taken in excess, and may cause allergies depending on the individual. Because it may be used, caution is required.

Recently, in order to solve the above problems while reducing side effects of the skin caused by various chemical substances, many compositions for external application for skin such as cosmetics using natural substances have been developed. Accordingly, the present inventors studied the possibility of application as an external preparation for skin from plants that have not been widely known so far.

KR 2002-0070566 A

Through repeated and various experiments, the inventors of the present specification confirmed that the extract of Gusang-gu is safe for the skin and can enhance the excellent moisturizing effect and skin immunity, and completed the present invention.

Accordingly, an object of the present specification is to provide a composition for external application for skin comprising a bulbous tree extract.

In order to achieve the above object, the present specification provides a composition for external application for skin for improving skin immunity and moisturizing the skin, comprising the extract of chinensis as an active ingredient.

The composition of the present specification exhibits a skin moisturizing effect and an immunity enhancing effect by including the extract of Gusang-gu, and through this, it can be used in various ways in cosmetics or medicine.

In one aspect, the present specification may relate to a composition comprising an extract of Abies Koreana as an active ingredient.

In one aspect of the present specification, the composition may be a pharmaceutical, food, or cosmetic composition.

In one aspect of the present specification, the composition may be a composition for improving skin.

In one aspect of the present specification, the skin condition improvement may be skin moisturizing, skin barrier function enhancement, anti-inflammatory, antibacterial, sebum secretion suppression or acne improvement, prevention or treatment.

In one aspect of the present specification, the composition may be a composition for improving, preventing or treating atopic dermatitis, xeroderma, or psoriasis.

In one aspect of the present specification, the antibacterial may be a composition that is antibacterial against acne bacteria. The acne bacteria may be propionibacterium acnes .

In one aspect of the present specification, the bulbous tree may be one or more selected from the group consisting of leaves, bark, and coniferous bulbous trees.

In one aspect of the present specification, the bulbous tree extract may be extracted by mixing leaves, conifers and bark at a weight ratio of 7:2:1. The weight ratio may be a weight ratio of 1 to 10:1 to 3:1 or a weight ratio of 6 to 8: 1.5 to 2.5:1.

In one aspect of the present specification, the bulbous tree extract may be one or more extracts selected from the group consisting of water, organic solvents, and mixtures thereof. The organic solvent may be one or more selected from the group consisting of ethanol, butylene glycol, and propylene glycol.

In one aspect of the present specification, the composition may be a composition for external application for skin.

In the present specification, the globular tree (Korean fir, Abies Koreana ) is a perennial evergreen coniferous arboreous tree of the pine family and grows wildly at 500 to 2,000 m above sea level in Mt. Halla, Mt. Jiris, Mt. Deogyu, Mt. It is used as a building material and furniture material, and is planted for ornamental purposes in parks, botanical gardens, and gardens. The bulbous tree used in this specification was sampled as a landscape tree planted at Amorepacific Institute of Technology.

The method of preparing the bulbous tree extract used in the present specification is not particularly limited, and in the present specification, the bulbous tree extract is a leachate obtained by leaching and transferring from the bulbous tree, a concentrate obtained by concentrating part or all of the leachate again, and the concentration again. It contains the extract produced by drying water, or the chemical substance itself exhibiting the main effect contained in the extract.

The bulbous tree used in the present specification may be included in the form of an extract, or may be included as a pulverized product of the herbal medicine itself, or a dry pulverized product of the herbal medicine, but is not limited thereto. In addition, the bulbous tree used in the present specification is not limited in its acquisition method, and may be cultivated and used or purchased and used, and some or all of the above-ground or root portion of wood may be used, and preferably all of the above-ground portion You can use Specifically, one or more selected from the group consisting of leaves, bark, and conifers of woody bulbous trees may be used.

In the present specification, “extract” includes all substances obtained by extracting components of natural substances, regardless of the extraction method, extraction solvent, extracted component, or form of the extract. It is a broad concept that encompasses all substances that can be obtained by processing or processing by a method.

In the present specification, the term "spinus tree extract" includes all substances obtained by extracting the components of the bulbous tree, regardless of the extraction method, extraction solvent, extracted component, or form of the extract, and heat and acid in the process of extracting the component (acid), base (base), including a material obtained through an extraction method including a process of treatment with enzymes, etc., and can also be obtained by extracting the material obtained by extracting the components of the bulbous tree and then processing or treating it in other ways. It is a broad concept that includes all substances in existence.

In one aspect of the present specification, the Gusang tree extract comprises the steps of washing and drying the Gusang tree; Solvent extraction of the dried bulbous tree; And it can be obtained through the step of filtering the extracted bulbous tree. In the above, the solvent includes at least one selected from the group consisting of water, an organic solvent, and a mixture of water and an organic solvent. In the above, water includes distilled water or purified water, and the organic solvent is selected from the group consisting of alcohol, acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone, and chloroform, for example lower alcohols of C ₁ to C ₅ Including one or more, but is not limited thereto.

The bulbous tree extract may include a bulbous tree-C ₁ ~C ₆ alcohol extract, and specifically, the alcohol may be methanol or ethanol.

In the present specification, the bulbous tree extract may be a crude extract of a solvent selected from the group consisting of water, C ₁ -C ₆ alcohol, and combinations thereof. The C ₁ -C ₆ alcohol may be specifically methanol. When extracting the bulbous tree with a solvent, it is preferable to extract by adding a solvent corresponding to about 5 to 15 times that of the bulbous tree, and specifically, it is preferable to extract by adding about 10 times the solvent, but is not limited thereto. Heat extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction may be used for the extraction, and there is no limitation as long as the extraction method is obvious to those skilled in the art. The extraction may be performed at room temperature, but for more efficient extraction, it may be performed under heated conditions, preferably at a temperature of about 40 to 100°C, more preferably about 80°C, but limited thereto. It is not. The extraction time may be preferably performed for about 2 to 4 hours, more preferably about 3 hours, but is not limited thereto, and may vary depending on conditions such as extraction solvent and extraction temperature. The extraction may be extracted once or more several times in order to obtain a larger amount of the active ingredient, preferably 1 to 5 times, more preferably 3 times continuous extraction and the combined extract may be used.

In the present specification, the Gusang tree extract may include a crude extract of Gusang tree as described above, and may be included as a soluble fraction of an organic solvent obtained by further extracting the crude extract with an organic solvent having a low polarity. As the organic solvent, hexane, methylene chloride, ethyl acetate, n-butanol, etc. may be used, but the present invention is not limited thereto. The extract extracted by the above method or a soluble fraction of the extract may be used as it is, but may be used in the form of an extract after filtration and concentrated, or freeze-dried after concentration to be used as a freeze-dried product.

In the present specification, the term "skin" refers to a tissue covering the body surface of an animal, and is a broad concept including a scalp as well as a tissue covering the body surface such as a face or body.

Gusang tree extract of the present specification is a step of slicing and drying the bulbous tree; Adding water, an organic solvent, or a combination thereof to the dried spheroid tree and performing reflux extraction; After depositing the extract, filtering through a filter cloth; Centrifuging the filtrate to separate the residue and the filtrate; It can be obtained by a method comprising a; step of concentrating the separated filtrate under reduced pressure to obtain a bulbous tree extract.

In one aspect of the present specification, the bulbous tree extract is composed of purified water, ethanol, butylene glycol, and propylene glycol so that the bulbous tree concentrate concentrated under reduced pressure according to the above method is included in 5% by weight based on the total weight of the extract. It may be prepared by dispersing/dissolving using one or more solvents selected from the group.

In addition, in the extraction process of the bulbous tree extract of the present specification, for example, water or an organic solvent was added to the minced and dried bulbous tree, followed by reflux extraction and immersion, and then the residue and filtrate are separated through filter cloth filtration and centrifugation, The separated filtrate can be concentrated under reduced pressure to obtain a bulbous tree extract. The organic solvent usable in the present specification may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform, or a mixed solvent of these organic solvents and water, and when considering the safety of raw materials, preferably 100% water, 70% ethanol Alternatively, butylene glycol (Butylene Glycol) is used. In this case, the extraction temperature is preferably 18 to 80°C for water, 18 to 40°C for 70% ethanol and butylene glycol, and the extraction time is preferably 6 to 168 hours. If the extraction temperature and extraction time are out of the above, extraction efficiency may decrease or a change in components may occur. After obtaining the extract by using the solvent above, the liquid may be obtained by cooling, heating, and filtration at room temperature by a conventional method known in the art, or the solvent may be further evaporated, spray dried, or freeze-dried. In addition, during lyophilization, freeze drying may be performed at -50 to -70°C for 3 to 4 days.

The composition of the present specification is preferably included in an amount of 0.001 to 50% by weight based on the total weight of the composition of the Gusang tree extract. More preferably 0.1 to 1% by weight or 0.5 to 10% by weight may be used. In the case of 0.001% by weight or less, it is difficult to expect the efficacy, and when 50% by weight is included, a problem in the formulation occurs. Specifically, the extract is 0.001% by weight or more, 0.01% by weight or more, 0.1% by weight or more, 0.3% by weight or more, 0.5% by weight or more, 0.8% by weight or more, 1% by weight or more, 3% by weight or more based on the total weight of the composition. , 5% by weight or more, 7% by weight or more, 10% by weight or more, 15% by weight or more, 20% by weight or more, 25% by weight or more, 30% by weight or more, 35% by weight or more, or 40% by weight or more, and 60% by weight % Or less or 50% by weight or less.

In one aspect of the present specification, the concentration of the bulbous tree extract may be 0.01ug/mL to 100ug/mL. In addition, the concentration of the bulbous tree extract is 0.01ug/mL or higher, 0.1ug/mL or higher, 0.3ug/mL or higher, 0.4ug/mL or higher, 0.5ug/mL or higher, 0.6ug/mL or higher, 0.7ug/mL or higher, It may be 1 ug/mL or more, 5 ug/mL or more, 10 ug/mL or more, 100 ug/mL or more, or 1000 ug/mL or more, and it may be 100 mg/mL or less or 10 mg/mL or less.

The pharmaceutical composition according to the present specification may be in various oral or parenteral dosage forms. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, soft or hard capsules, and the like, and these solid preparations include at least one excipient, such as starch, calcium carbonate, and sucrose, in one or more compounds. Or it is prepared by mixing lactose, gelatin, and the like. Also, in addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspending agents, intravenous solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used as diluents, various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, can be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, and suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin butter, and glycerogelatin may be used.

The pharmaceutical dosage form of the composition of the present specification may be used in the form of a pharmaceutically acceptable salt thereof, and may be used alone or in combination with other pharmaceutically active compounds, as well as in an appropriate set. The salt is not particularly limited as long as it is pharmaceutically acceptable, for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid. , Benzenesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid, and the like can be used.

The composition of the present specification may be administered parenterally or orally as desired, and may be administered in divided doses from 1 to several times so as to be administered in an amount of 0.1 to 500 mg, preferably 1 to 100 mg per 1 kg of body weight per day. have. The dosage for a specific patient may vary depending on the patient's weight, age, sex, health status, diet, administration time, administration method, excretion rate, disease severity, and the like.

The pharmaceutical compositions according to the present specification, respectively, according to a conventional method, oral formulations such as powders, granules, tablets, soft or hard capsules, suspensions, emulsions, syrups, aerosols, skin external preparations such as ointments, creams, suppositories, injections And it may be used by formulating in any form suitable for pharmaceutical preparations, including sterile injectable solutions.

The composition according to the present specification can be administered to mammals such as rats, mice, livestock, humans, etc. by various routes such as parenteral and oral, and all modes of administration can be expected, for example, oral, rectal Alternatively, it may be administered by intravenous, intramuscular, subcutaneous, intrauterine dura mater or by intracerebroventricular injection.

In one aspect of the present specification, the food composition may be a health functional food composition.

The formulation of the food composition according to the present specification is not particularly limited, but may be formulated as, for example, a tablet, granule, powder, liquid such as a drink, caramel, gel, bar, or the like. In the food composition of each formulation, in addition to the active ingredient, ingredients commonly used in the field may be appropriately selected and blended by a person skilled in the art according to the formulation or purpose of use without difficulty, and synergistic effects may occur when applied simultaneously with other ingredients.

In the food composition according to the present specification, the determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage thereof is, for example, 0.1 mg/kg/day to 5000 mg/kg/day, more specifically 50 It may be from mg/kg/day to 500 mg/kg/day, but is not limited thereto, and may vary depending on various factors such as age, health status, and complications of the target to be administered.

The food composition according to the present specification is, for example, various foods such as chewing gum, caramel products, candies, frozen desserts, and confectionery, beverage products such as soft drinks, mineral water, alcoholic beverages, and health functional foods including vitamins and minerals. I can.

In addition to the above, the food composition, which is an aspect of the present specification, includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, and the like. In addition, the functional food compositions of the present specification may include flesh for the manufacture of natural fruit juice and fruit juice beverage and vegetable beverage. These components may be used independently or in combination. The proportion of these additives is not so critical, but is generally included in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition herein.

The cosmetic composition according to an aspect of the present specification is not particularly limited in formulation, and may be appropriately selected according to the purpose. For example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing Foam, cleansing lotion, cleansing cream, body lotion, and may be prepared in one or more formulations selected from the group consisting of a body cleanser, but is not limited thereto.

When the formulation of the cosmetic composition according to the present specification is a paste, cream, or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as carrier components And the like can be used.

When the formulation of the cosmetic composition according to the present specification is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluoro Propellants such as hydrocarbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition according to the present specification is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition according to the present specification is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like may be used.

In the case of a surfactant-containing cleansing of the cosmetic composition according to the present specification, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives, or ethoxylated glycerol fatty acid esters may be used.

The cosmetic composition according to the present specification may additionally include functional additives and ingredients included in general cosmetic compositions in addition to the extract of chinensis. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

In the cosmetic composition of the present specification, in addition to the functional additives, components included in general cosmetic compositions may be blended if necessary. Other components included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation Accelerators, cooling agents, limiting agents, purified water, and the like.

In addition, the present specification relates to a skin external preparation comprising the extract of Gusang-gu as an active ingredient, and the external preparation for skin is a generic term that may include anything applied outside the skin, and cosmetics of various formulations may be included therein.

Hereinafter, the configuration and effects of the present specification will be described in more detail with reference to Examples, Test Examples, and Preparation Examples. However, these Examples, Test Examples, and Preparation Examples are provided for illustrative purposes only to aid understanding of the present specification, and the scope and scope of the present specification are not limited by the following examples.

[Preparation Example 1] Preparation of bulbous tree extract

?A total of 1 kg of leaves, conifers, and bark of a shredded and shaded bulbous tree were refluxed three times for 24 hours with a 70% ethanol aqueous solution at a weight ratio of 7:2:1, and cooled, and then Whatman #5 filter paper Filtered. The filtered extract was concentrated under reduced pressure at 45° C. or lower and freeze-dried at -60° C. for 3 days.

A bulbous tree extract was prepared by dispersing/dissolving with ethanol so that the vacuum concentrate was contained in an amount of 5% by weight based on the total weight of the bulbous tree extract.

[Example 1 and Comparative Example 1]

In order to use the composition of the present specification, a composition comprising the extract of the bulbous tree of Example 1 was prepared as a cream according to the composition specified in Table 1 below. For comparison, a cream formulation that did not contain bulbous tree extract was used as a comparative example.

ingredient Example 1 (% by weight) Comparative Example 1 (% by weight) Purified water to 100 to 100 Gobula tree extract of Preparation Example 1 0.5 - Hydrogenated vegetable oil 1.5 1.5 Stearic acid 0.6 0.6 Stearyl alcohol 2.0 2.0 Glycerol stearate 1.0 1.0 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.0 1.0 Arachidyl Behenyl Alcohol & Arachidyl Glucoside 1.0 1.0 Cetearyl Alcohol & Cetearyl Glucoside 2.0 2.0 PEG-100 Stearate & Glycerololeate & Propylene Glycol 1.5 1.5 Caprylic/Capric Triglyceride 11 11 Cyclomedicone 6 6 Triethanolamine 0.1 0.1

[Comparative Example 2]

In Preparation Example 1, a willow extract was obtained through the same method using the bulbous tree as willow.

[Test Example 1] Skin moisturizing effect test

In order to find out the skin moisturizing persistence when applied once, the cosmetic composition of Example 1 and Comparative Example 1 was applied to the face and the inside of the entire skin to 50 men and women in their 30s to 40s who complained of dryness or thought to be dry skin. After that, the skin moisture content was measured using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) for 24 hours. The coniometer measures the amount of moisture present in the epidermis of the skin by using a sensor to measure the ionic level of moisture and calculates the amount of moisture by quantifying it, and the measurement method is as follows.

1) Place the coniometer probe on the skin area to be measured.

2) When the probe is pressed on the skin, the capacitance of the skin is quantified through a sensor and displayed on the screen.

3) The measurement was repeated by changing the measurement site.

4) After measuring once, the sensor was wiped with a tissue such as Kimwipe, and then measured again.

In the above method, the skin moisture content was measured using a coniometer under constant temperature and humidity conditions (24° C., humidity 40%) before the start of application, as a basic value, and changes after 12 and 24 hours were measured. The results are shown in Table 2 below.

Skin moisture content (%) sample Before application 12 hours later After 24 hours Example 1 24.3 37.2 34.0 Comparative Example 1 25.4 33.1 26.0

From the results of Table 2, when Comparative Example 1 was applied, the skin moisture content increased to some extent after 12 hours after application, but returned to the state before application after 24 hours. On the other hand, it can be seen that when Example 1, which is a composition for external application for skin containing the extract of chinensis, is applied to the skin, its moisturizing power lasts for more than 24 hours and the effect of increasing the skin moisture content is also higher than that of Comparative Example 1.

Therefore, it can be seen that the composition for external application for skin comprising the extract of Gusangja tree according to one aspect of the present specification has a remarkable skin moisturizing effect.

[Test Example 2] Evaluation of the effect of improving dry skin (or psoriasis)

At the end of Test Example 1, subjective efficacy evaluation was conducted through a questionnaire on the effect of improving dry skin after using Example 1 and Comparative Example 1 for test subjects. In the questionnaire survey, satisfaction with the degree of skin dryness improvement effect was classified into very good, good, moderate and poor, and the results are shown in Table 3 below.

Number of sample respondents Very good Good usually Inadequate Example 1 8 28 12 2 Comparative Example 1 5 20 18 7

As can be seen from the results of Table 3, Comparative Example 1 is the number of people marked as good or higher, while the number of people, Example 1, which is a composition for external application for skin comprising the extract of Gusang-gu according to the present specification, is 36, and Comparative Example 1 Therefore, compared to Comparative Example 1, it can be seen that Example 1, which is a composition for external application for skin comprising the extract of Gusan, is superior in improving, preventing, or treating dry skin (or psoriasis). have.

[Test Example 3] Evaluation of atopic dermatitis improvement effect

Subjective efficacy evaluation was performed through a questionnaire on the effect of improving dry skin after using Example 1 and Comparative Example 1 for patients with atopic dermatitis acutely worsened. The use of Example 1 and Comparative Example 1 was applied once in the morning and then once in the evening according to the user's life pattern. At the time of application, the cream was applied directly to the skin as if using a cosmetic cream, and the cream was applied to the area where symptoms of atopic dermatitis appeared. One identical examiner evaluated the patient's degree of improvement of skin lesions and SCORAD (SCORing Atopic Dermatitis) score, and the patient recorded a visual analogue scale (VAS, 0-10 cm) to evaluate the severity of itching. I did. The evaluation and recording were made within 1 hour after application. After the experiment, the results are shown in Table 4 below.

Before application After one application After application twice SCORAD score 53.10 41.80 36.50 VAS Score 9 6 4

In the case of a SCORAD score of 40 or higher, it is judged that atopic symptoms are serious. As can be seen from the above results, when the composition for external application for skin of Example 1 was applied, the score from 53.10 decreased to 36.50 and decreased to 40 or less, thereby confirming that the atopic state was improved.

In addition, in the case of the VAS score, the closer to 10, the more severe the degree of pain. It can be seen that from 9 points before application, to 4 points after application twice. It can be judged that the pain that the patient feels due to atopic dermatitis has significantly decreased.

Therefore, it was found that the SCORAD score and the VAS score were all decreased by repeating the application of Example 1 to improve, prevent, and treat overall atopic dermatitis symptoms including body inflammatory response and itchiness, and exhibit excellent skin moisturizing effects. I can.

[Test Example 4] Antibacterial activity test against acne bacteria

With the compositions of Example 1 and Comparative Example 1, antibacterial activity was tested against Propionibacterium acnes <ATCC 6919: medium-BHI broth>, which is a strain causing acne.

The test method for antibacterial activity against acne bacteria was as follows.

(1) Preparation of test bacteria solution

Propionibacterium acnes was inoculated into BHI broth and anaerobic cultured.

(2) Preparation of diluted solution

0.15 ml of the test bacteria solution was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5), and then well mixed was used as a diluted solution.

(3) Sample preparation

The cream, which is a cosmetic composition prepared from Example 1 and Comparative Example 1, was used as a sample as it was.

(4) Antibacterial test

1) Put the sample to match the starting concentration of 0.4% in the 96-well cell culture tube (96-well plate) row 1, and adjust the total amount to 200ul with the diluted solution. Add 100ul of diluted solution to the remaining wells.

2) After mixing the mixed solution in row 1 well, take 100ul, put it in row 2, mix well, then take 100ul again and put it in row 3, and double dilution.

3) After stationary culture at 32°C for 24 hours and 48 hours, the presence or absence of bacterial growth is determined to the degree of suspension, and the minimum concentration without bacterial growth is determined as the MIC (minimum inhibitory concentration) value. If the mixed solution is opaque and it is difficult to determine the presence or absence of bacterial growth, check through microscope observation.

The results of the antimicrobial activity test against acne bacteria are shown in Table 5 below.

sample pH Propionibacterium acnes Example 1 5.37 <5ppm Comparative Example 1 5.63 <10ppm

Example 1 had an antimicrobial activity of 5 ppm MIC against the test bacteria. The lower the ppm concentration in the MIC, the more effective it can be against acne bacteria at a lower concentration, so it can be judged that the antibacterial effect of acne bacteria is more excellent. Therefore, it was confirmed that Example 1 exhibits a remarkable effect that is twice as high in antibacterial of acne bacteria since the MIC concentration for inhibiting Propionibacterium acnes is twice lower than that of Comparative Example 1. Therefore, it can be confirmed that the composition comprising the extract of the bulbous tree according to the present specification has an excellent antibacterial effect against acne bacteria.

[Test Example 5] Acne improvement, sebum secretion reduction, and test with or without irritation

Twelve people with acne were allowed to use the cream, a cosmetic composition prepared from Example 1 and Comparative Example 1, for one month. The acne improvement scale was set from 1 to 5 points, with 1 point as ‘No,’, 3 points as ‘normal’, and 5 points as ‘very yes.’ The experimental results are expressed as the average score of 12 people in Table 6 below.

Acne disappearance time was based on the number of days that disappearance was read, and acne recurrence was based on the result after 1 month with or without acne. The reduction in sebum secretion was set from 1 to 5 points, and 1 point was marked as ‘No,’ for 3 points, ‘It is normal,’ and 5 points for ‘Very well.’ The experimental results are expressed as the average score of 12 people in Table 6 below. The presence or absence of skin irritation was considered as (number of persons with irritation reaction)/(total number of test subjects).

Improvement of inflammatory acne When to disappear from acne Acne recurrence Decreased sebum secretion Presence or absence of stimulation Example 1 4.37 4 days radish 4.03 1/12 Comparative Example 1 3.1 8 days U 2.97 3/12

As shown in Table 6, in Example 1, compared to Comparative Example 1, acne did not recur, an average of 4 points or more for improvement of inflammatory acne, an average of 4 days or less for the time of disappearance of comedonic acne, and an average of reduction of sebum secretion. By obtaining more than 4 points, it can be seen that it has excellent effects on acne improvement and prevention treatment. In addition, it can be seen that it has an excellent effect on suppressing sebum secretion.

Therefore, it can be seen that the extract of vulgaris is suitable for patients with acne skin when looking at various factors such as irritation, improvement speed, degree of improvement, and degree of sebum secretion reduction.

[Test Example 6] Inflammation improvement effect

The anti-inflammatory effect was evaluated by the inhibitory effect of prostaglandin production. The effect was measured on macrophages (purchased from ATCC) using the previously extracted extract. First, aspirin was added to the macrophages collected from the peritoneal cavity of the mouse to a final concentration of 500M to irreversibly inhibit the activity of cyclooxygenase (“COX”) remaining in the cells. Then, 100 µl of the suspension was added to each well of a 96-well cell culture tube and incubated for 2 hours in an incubator under conditions of 5% CO2 and 37°C to attach macrophages to the surface of the container. Subsequently, the attached macrophages were washed three times with PBS and then used for the effect test of the extract. Roswell Park Memorial Institute medium (RPMI) containing 1% (w/v) LPS was added to 5×10 4 cells/ml of previously cultured macrophages and cultured for 12 hours, followed by inducing prostaglandin production and preparation By treating 100 μl of the bulbous tree extract of Example 1 and the willow tree extract of Comparative Example 2 at a concentration of 0.5 ug/mL, the free prostaglandin was quantified using an enzyme immunoassay (ELISA). At this time, the prostaglandin production inhibitory activity of the extract is set to 100% difference between the prostaglandins produced in the LPS-treated group and the non-treated group, and treated with LPS and the sample to reduce the percentage of prostaglandin. Comparison and determination were made, and the results (effect of inhibiting prostaglandin production) are shown in Table 7 below.

Blank 100% Control group (aspirin treatment group) 25.0% Preparation Example 1 35.4% Comparative Example 2 21.7%

As shown in Table 7, the experimental results showed that the inhibitory effect of production of prostaglandin according to Preparation Example 1 was very high compared to the control group treated with aspirin. In addition, in the case of Preparation Example 1, which is a bulbous tree extract according to the present specification, it was found that the inhibitory effect of prostaglandin production was very high even when compared with Comparative Example 2, which is a willow extract. Therefore, it was confirmed that the extract of Gusangja tree according to the present specification exhibits an excellent anti-inflammatory effect.

An example of the formulation of the composition according to an aspect of the present specification is described below, but it can be applied to various other formulations, which is not intended to limit the present specification, but is intended to be described in detail.

[Formulation Example 1] Soft capsule

Gobula tree extract of Preparation Example 1 8mg, vitamin E 9mg, vitamin C 9mg, palm oil 2mg, hydrogenated vegetable oil 8mg, sulfur wax 4mg and lecithin 9mg are mixed, and mixed according to a conventional method to prepare a soft capsule filling solution. Each capsule is filled with 400 mg to prepare a soft capsule. In addition to the above, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin, and 10 parts by weight of sorbitol liquid, and the filling solution was filled to prepare a soft capsule containing 400 mg of the composition according to the present specification.

[Formulation Example 2] Tablet

After mixing 8 mg of Gobula tree extract of Preparation Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of malt sugar, and granulated using a fluid bed dryer, 6 mg of sugar ester Add. 500 mg of these compositions are tableted by a conventional method to prepare tablets.

[Formulation Example 3] Drink agent

After mixing 8 mg of Gusang tree extract of Preparation Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide, 300 ml of purified water was added and each bottle was filled with 200 ml each. After filling the bottle, sterilize at 130°C for 4-5 seconds to prepare a drink.

[Formulation Example 4] Granules

After mixing 8 mg of the extract of Gujugo serrata, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous crystalline glucose and 550 mg of starch of Preparation Example 1, the granules were formed into granules using a fluid bed granulator, and then filled into a bag to prepare granules.

[Formulation Example 5] Injection

According to the composition shown in Table 8 below, an injection was prepared by a conventional method.

Ingredients content Gobula tree extract of Preparation Example 1 10-50 mg Sterile distilled water for injection Appropriate amount pH adjuster Appropriate amount

[Formulation Example 6] Health Functional Food

According to the composition shown in Table 9 below, a health functional food was prepared by a conventional method.

Ingredients content Gobula tree extract of Preparation Example 1 20mg Vitamin A acetate 70μg Vitamin E 1.0mg Vitamin B1 0.13mg Vitamin B2 0.15mg Vitamin B6 0.5mg Vitamin B12 0.2μg Vitamin C 10mg Biotin 10μg Nicotinic acid amide 1.7mg Folic acid 50μg Calcium pantothenate 0.5mg Ferrous sulfate 1.75mg Zinc oxide 0.82mg Magnesium carbonate 25.3mg Potassium monophosphate 15mg Dicalcium phosphate 55mg Potassium citrate 90mg Calcium carbonate 100mg Magnesium chloride 24.8mg

The composition ratio of the vitamin and mineral mixture is a mixture of components suitable for a health functional food in a preferred embodiment, but the mixing ratio may be arbitrarily modified.

[Formulation Example 7] Healthy Drink

A health drink was prepared by a conventional method according to the composition shown in Table 10 below.

Ingredients content Gobula tree extract of Preparation Example 1 1000mg Citric acid 1000mg oligosaccharide 100 g Taurine 1g Purified water Balance

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and sterilized.

[Formulation Example 8] Flexible lotion (skin lotion)

According to the composition shown in Table 11 below, a flexible lotion was prepared by a conventional method.

Ingredients Content (% by weight) Gobula tree extract of Preparation Example 1 0.2 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 PEG-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservatives, colors, flavors Appropriate amount Purified water Balance

[Formulation Example 9] Nutritional lotion (milk lotion)

Nutrient cosmetic water was prepared by a conventional method according to the composition shown in Table 12 below.

Ingredients Content (% by weight) Gobula tree extract of Preparation Example 1 1.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Beeswax 4.0 Polysorbate 60 1.5 Caprylic/Capric Triglyceride 5.0 Squalane 5.0 Sorbitasesquioleate 1.5 Floating paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservatives, colors, flavors Appropriate amount Purified water Balance

[Formulation Example 10] Nourishing Cream

A nutrient cream was prepared by a conventional method according to the composition shown in Table 13 below.

Ingredients Content (% by weight) Gobula tree extract of Preparation Example 1 2.0 glycerin 3.0 Butylene glycol 3.0 Floating paraffin 7.0 Beta glucan 7.0 Carbomer 0.1 Caprylic/Capric Triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan Stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservatives, colors, flavors Appropriate amount Purified water Balance

[Formulation Example 11] Massage Cream

A massage cream was prepared by a conventional method according to the composition shown in Table 14 below.

Ingredients Content (% by weight) Gobula tree extract of Preparation Example 1 2.0 glycerin 8.0 Butylene glycol 4.0 Floating paraffin 45.0 Beta glucan 7.0 Carbomer 0.1 Caprylic/Capric Triglyceride 3.0 Beeswax 4.0 Cetearyl glucoside 1.5 Sesqui oleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservatives, colors, flavors Appropriate amount Purified water Balance

[Formulation Example 12] Pack

A pack was prepared by a conventional method according to the composition shown in Table 15 below.

Ingredients Content (% by weight) Gobula tree extract of Preparation Example 1 0.2 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Preservatives, colors, flavors Appropriate amount Purified water Balance

As described above, specific parts of the present specification have been described in detail, and it is clear that these specific techniques are only preferred embodiments, and the scope of the present specification is not limited thereto for those of ordinary skill in the art. Accordingly, the substantial scope of the present specification will be defined by the appended claims and their equivalents.

Claims (12)

A cosmetic composition for suppressing sebum secretion comprising an extract of Abies Koreana as an active ingredient. The composition of claim 1, wherein the bulbous tree is at least one selected from the group consisting of leaves, bark, and coniferous bulbous trees. The composition of claim 1, wherein the bulbous tree extract is at least one extract selected from the group consisting of water, organic solvents, and mixtures thereof. The composition of claim 3, wherein the organic solvent is at least one selected from the group consisting of ethanol, butylene glycol, and propylene glycol. The composition of claim 1, wherein the bulbous tree extract is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition. The composition according to claim 5, wherein the bulbous tree extract is contained in an amount of 0.01 to 1% by weight based on the total weight of the composition. A food composition for suppressing sebum secretion comprising an extract of Abies Koreana as an active ingredient. The composition of claim 7, wherein the bulbous tree is at least one selected from the group consisting of leaves, bark and coniferous bulbous trees. The composition of claim 7, wherein the bulbous tree extract is one or more extracts selected from the group consisting of water, organic solvents, and mixtures thereof. The composition of claim 9, wherein the organic solvent is at least one selected from the group consisting of ethanol, butylene glycol, and propylene glycol. The composition according to claim 7, wherein the bulbous tree extract is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition. 12. The composition of claim 11, wherein the extract is contained in an amount of 0.01 to 1% by weight based on the total weight of the composition.