KR102576424B1 - The composition comprising extract of Rhamnus davurica for improving acne - Google Patents

Abstract

The present invention relates to a composition containing sea buckthorn extract with an acne improving and preventing effect, and specifically, to a composition containing sea buckthorn extract, which has an antibacterial effect against the Propionibacterium acnes bacterium, as an active ingredient. It relates to a composition for preventing and improving acne.

Description

Composition for preventing and improving acne comprising extract of sea buckthorn tree {The composition comprising extract of Rhamnus davurica for improving acne}

The present invention relates to a composition for preventing and improving acne containing sea buckthorn extract, and more specifically, to preventing acne containing sea buckthorn extract as an active ingredient, which has an antibacterial effect against Propionibacterium acnes. and compositions for improvement.

Acne (Acne vulgaris) is one of the most common skin diseases that occurs from adolescence to middle age, and is caused by various factors such as abnormal hyperkeratinization of the hair follicle epithelium, inflammation induced by Propionibacterium acnes, and sebum hypersecretion. It acts as the cause. Sebum is secreted from sebaceous glands that are connected to hair-containing ducts called hair follicles. Sebaceous glands are abundant in the face, back, and chest areas, so acne commonly occurs in these areas. In normal skin, sebum is discharged to the skin surface through the opening of the hair follicle, but if abnormal keratinization of the hair follicle or sebum hypersecretion causes blockage of the hair follicle and does not allow sebum to be discharged properly, comedones, the basic lesion of acne, may form.

Symptoms of acne range from comedonal acne, where excessive secretion of sebum accumulates in pores around the sebaceous glands, forming comedones, to papules, which are red acne when the acne bacteria Propionibacterium acnes proliferates and an inflammatory response occurs. Papular acne, where the inflammatory reaction of the papules becomes more severe, causes pustules, which are pockets of pus, to form inside, and nodular acne, where the pustules burst inside the skin and cause more inflammation around the acne affected area, forming finger-sized nodules. It appears as a variety of skin lesions. Acne is a common skin disease, so when the symptoms are mild, such as comedonal acne, it is considered a temporary physiological phenomenon. However, in cases such as purulent or nodular acne, which shows damage to surrounding tissues and infiltration of inflammatory cells, it is likely to leave scars or pigmentation.

Methods such as taking oral antibiotics and retinoids are commonly used to treat acne. In particular, in the case of inflammatory acne caused by bacterial infection, symptoms can be alleviated through antibiotic treatment, but various side effects have been reported, and bacterial resistance to the majority of these antibiotics is rapidly emerging. Recently, tetracycline, erythromycin, etc. are antibiotics used to inhibit the growth of Propionibacterium acnes and reduce the inflammatory response, but continuous use is difficult due to the development of antibiotic resistance and severe hepatotoxicity when used in large doses. It is known that In addition, benzoyl peroxide, which is mainly used as a topical treatment, has a strong antibacterial effect against Propionibacterium acnes and has anti-inflammatory and comedonolytic effects. However, there is a risk of serious irritation to the skin and various side effects such as redness, rash, and dryness. Therefore, in order to overcome these problems, research is being actively conducted on the development of effective substances using natural substances that are relatively less irritating to the skin and have fewer side effects.

The purpose of the present invention to solve the above problems is to provide a composition that is effective in preventing and improving acne, including buckthorn extract, which has an antibacterial effect, as an active ingredient.

Aspects of the present invention for achieving the above object are:

It is a composition for preventing and improving acne containing sea buckthorn extract as an active ingredient,

The sea buckthorn extract provides a composition for preventing and improving acne, which is one or more extracts selected from the group consisting of the stems, leaves, flowers, fruits and seeds of the sea buckthorn tree.

According to the present invention, a composition for preventing and improving acne containing sea buckthorn extract has antibacterial activity against Propionibacterium acnes and is effective in preventing and improving acne.

In addition, the composition for preventing and improving acne is made of natural substances, so there are no side effects or risks caused by synthetic substances, and it is harmless and safe to the human body.

Figure 1 is a photograph showing the results of an antibacterial activity test according to treatment of sea buckthorn leaf extract with or without treatment.
Figure 2 is a graph showing the degree of inhibition of bacterial growth according to the concentration of sea buckthorn leaf extract.
Figure 3 is a graph showing the results of a cytotoxicity test of sea buckthorn leaf extract on skin keratinocytes.

Hereinafter, the present invention will be described in more detail. However, this is only specifically described to facilitate understanding of the present invention, and the scope of the present invention is interpreted based on the claims.

The present invention is a composition for preventing and improving acne containing sea buckthorn extract as an active ingredient, and the sea buckthorn extract is one or more extracts selected from the group consisting of the stems, leaves, flowers, fruits and seeds of the sea buckthorn tree. and compositions for improvement.

The buckthorn tree (Rhamnus davurica) is a deciduous shrub belonging to the Buckthorn family and grows naturally in the Baekdudaegan mountain range of the Korean Peninsula. The fruit of the buckthorn tree is called Seorija (鼠李子), the bark is called Seoripi (鼠李皮), and the roots are called Seorigeun (鼠李根), and it is used medicinally as a diuretic and laxative. Additionally, the bark and fruit contain a yellow pigment, so they are used as a dye and are used to prevent and treat bone diseases.

In the present invention, the sea buckthorn extract may be one or more selected from the group consisting of sea buckthorn stem extract, sea buckthorn leaf extract, sea buckthorn flower extract, sea buckthorn fruit extract, and sea buckthorn seed extract, depending on the part used.

In the present invention, the “buckthorn stem extract” refers to all types of extracts obtained by extracting sea buckthorn stems, for example, extracts in the form of essence obtained by directly or indirectly heating the plant stem, and extracts of living plant stems. It can include extracts in the form of a sap that flows out by cutting the upper part, extracts obtained by extracting plants with organic solvents or water, and extracts obtained by cooling the gas when burning plant stems.

The “buckthorn fruit extract” may include an extract obtained using an organic solvent or water, an extract in the form of a liquid obtained by pressing sea buckthorn fruit, and an oil extract obtained by depressurizing a solvent extract.

The “buckthorn seed extract” may include an extract obtained using an organic solvent or water, a sap extract obtained by pressing sea buckthorn seeds, or an oil extract obtained by depressurizing a solvent extract.

The “buckthorn leaf extract” refers to an extract obtained using an organic solvent or water.

The “buckthorn flower extract” refers to an extract obtained using an organic solvent or water.

In one embodiment of the present invention, the sea buckthorn extract can be extracted using water, an organic solvent, or a mixed solvent thereof, and it is preferable to extract it using an organic solvent.

The organic solvent may be one or more selected from the group consisting of alcohols having 1 to 4 carbon atoms, ethyl acetate, hexane, and chloroform.

In one embodiment of the present invention, an ethanol extract of sea buckthorn leaves may be used as a preferred extract.

In the present invention, the sea buckthorn extract can be extracted using a cold needle extraction method, a warm needle extraction method, or a heat extraction method, and a conventional extraction device, an ultrasonic grinding extractor, or a fractionator can be used.

In addition, the extract may be an oil extract obtained by concentrating the extract of the buckthorn fruit extract and the buckthorn seed extract with water, an organic solvent, or a mixed solvent thereof under reduced pressure.

In one embodiment of the present invention, the prepared extract may be filtered, concentrated, or dried to remove the solvent. Specifically, the filtration may be performed using filter paper or a reduced pressure filter, and the concentration may be performed using a reduced pressure concentrator, such as It can be concentrated under reduced pressure using a rotary evaporator, and the drying can be performed by, for example, a freeze-drying method.

In the present invention, the composition for preventing and improving acne may have antibacterial activity against Propionibacterium acnes.

Propionibacterium acnes, the causative agent of acne, is an anaerobic Gram-positive bacterium that does not form spores and has a rod shape, so it is also called acne bacillus. Propionibacterium acnes is capable of limited aerobic growth and is one of the representative bacterial flora of the skin and hair follicles. Propionibacterium acnes causes inflammatory acne by promoting the production of pro-inflammatory cytokines in keratinocytes, sebocytes, and macrophages. Interactions between Propionibacterium acnes and infiltrating monocytes and lymphocytes are also known to play an important role in the development of inflammatory acne. Propionibacterium acnes produces low molecular weight peptides, which chemically attract polymorphonuclear leukocytes (PMNK) and cause inflammation. Various inflammatory linking factors stimulate immune cells to produce inflammatory cytokines such as IL-8, IL-1b, and TNF-a, deepening the inflammatory response in the skin. When hair follicle occlusion occurs due to abnormal keratinization or sebum hypersecretion, oxygen inflow into the hair follicle is reduced, which creates more favorable conditions for the growth of anaerobic Propionibacterium acnes, which proliferates in acne lesions. It is promoted. In addition, when sebum secretion increases from the sebaceous glands, the growth of lipophilic Propionibacterium acnes becomes favorable, and the lipase enzyme produced by Propionibacterium acnes breaks down sebum. This forms fatty acids. Fatty acids produced by Propionibacterium acnes stimulate hair follicles, destroying the hair follicle walls and causing immune cells to flock to the area and cause inflammation.

The composition for preventing and improving acne contains sea buckthorn extract as an active ingredient and exhibits excellent effects in preventing and improving acne by inhibiting the growth of Propionibacterium acnes.

In one embodiment of the present invention, the sea buckthorn extract may be included in an amount of 0.001 to 5.0% by weight, preferably 0.02 to 0.2% by weight, based on the total weight of the composition.

If the content is less than 0.001% by weight, an effective effect cannot be shown, and if it exceeds 5.0% by weight, there are problems with formulation stability such as discoloration and off-odor.

In the present invention, the composition for improving and preventing acne may further include various ingredients depending on the method and purpose of use in addition to the sea buckthorn extract.

According to one embodiment of the present invention, the composition for preventing and improving acne containing the sea buckthorn extract as an active ingredient may be prepared and used in one or more formulations selected from the group consisting of skin, lotion, cream, and powder. It is not limited to this.

Hereinafter, the present invention will be described in detail through experimental examples and examples to specifically explain the present invention. However, the experimental examples according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the experimental examples and examples described in detail below. Experimental examples and examples of the present invention are provided to more completely explain the present invention to those skilled in the art.

Preparation Example 1: Preparation of sea buckthorn extract

Dried sea buckthorn leaves were pulverized, immersed in twice the amount of ethanol, and exposed to an ultrasonic device for 30 minutes. After immersion for 24 hours, the extract was prepared by filtering through filter paper and removing the solvent using a rotary vacuum concentrator.

Experimental Example 1: Strains and culture conditions

The strain used was Propionibacterium acnes (KCTC 3314) purchased from the KCTC Biological Resources Center.

The distributed Propionibacterium acnes bacteria were inoculated into Reinforced Clostridial broth medium and then cultured for 72 hours at 37°C under anaerobic conditions and used in experiments.

Experimental Example 2: Confirmation of antibacterial activity - plate medium diffusion method

Agar diffusion method was performed to measure the antibacterial activity of sea buckthorn leaf extract against Propionibacterium acnes. Approximately 1 x 10^6 Propionibacterium acnes was uniformly applied per plate of Reinforced Clostridial Agar. An 8 It was incubated for 72 hours.

Antibacterial activity was determined by measuring the diameter of the clear zone in the experimental group compared to the extract-untreated control group.

As shown in Figure 1, the experimental group treated with the sea buckthorn leaf extract of the present invention showed an average clear zone of 12.75 mm.

Experimental Example 3: Confirmation of antibacterial activity - minimum inhibitory concentration (MIC) test

Extracts according to each concentration were treated with bacterial culture medium at a concentration of approximately 5x10^5 CFU/mL so that the final mixture was 200 uL in a 96 well-plate. In the case of the extract, a reduced-pressure concentrated sea buckthorn leaf extract dissolved in DMSO at a concentration of 50 mg/mL is used and diluted with sterilized liquid medium to a concentration of 2.0, 1.0, 0.5, 0.25, 0.125, and 0.0625 mg/mL. I was told to do it.

A 96-well plate treated with the above mixed solution containing bacterial culture and extract was cultured at 37°C for 72 hours under anaerobic conditions.

Determination of antibacterial activity was carried out by measuring the absorbance of the experimental group compared to the extract untreated control group at 600 nm using a microplate reader immediately after sample treatment and after 72 hours of incubation, and calculating and comparing the change value.

Figure 2 shows the degree of growth inhibition by concentration of sea buckthorn leaf extract against Propionibacterium acnes, and the MIC value of sea buckthorn leaf extract against Propionibacterium acnes is 0.25. It was confirmed that it was mg/mL.

Experimental Example 4: Cytotoxicity to skin keratinocytes

An MTT experiment was conducted to measure the toxicity of sea buckthorn leaf extract to skin keratinocytes.

HaCaT cells were treated at about 1x10^4 cells/well in a 96 well plate and then cultured in DMEM, 10% FBS, 1% penicillin-streptomycin medium at 37°C and 5% CO ₂ for 24 hours. After 24 hours, the medium used in the previous culture was removed, and the medium was treated with buckthorn extract diluted to 100, 50, 25, and 12.5 ug/mL and reacted at 37°C and 5% CO ₂ for 24 hours.

After treatment, 20 uL of 5 mg/mL MTT solution was added to each well and reacted at 37°C and 5% CO ₂ for 3 hours. After reaction, all supernatants were removed, 150 uL of DMSO was added at a time, reacted at room temperature for 10 minutes, and absorbance was measured at 540 nm using a microplate reader.

As a result, as shown in Figure 4, buckthorn leaf extract did not show cytotoxicity to skin keratinocytes at less than 200 ug/mL.

According to the above experimental results, it was confirmed that the sea buckthorn extract according to the present invention is a natural material that inhibits the growth of Propionibacterium acnes, the cause of acne, and has antibacterial activity, preventing and improving acne. .

Below, based on the results of the above experimental examples, several formulation examples using sea buckthorn extract are prepared and presented. However, these formulation examples are for illustrating the present invention, and it is obvious to those skilled in the art that the formulation of the present invention is not limited to these formulation examples.

Formulation Examples 1 to 3: Preparation of a cosmetic composition containing sea buckthorn extract

Formulation Example 1: Lotion (emulsion)

Ingredient name Example (% by weight) stearic acid 0.5 ethanol 10.0 vaseline 1.0 squalane 2.5 liquid paraffin 6.0 Polyoxyethylene sorbitan monooleate 1.5 1,3-butylene glycol 8.0 Triethanolamine 0.05 Spices 0.2 antiseptic Appropriate amount antioxidant Appropriate amount Sea buckthorn leaf extract 0.025 Purified water To 100

Formulation Example 2: Soft lotion (skin toner)

Ingredient name weight% glycerin 5.0 1,3-butylene glycol 3.0 ethanol 5.0 Polyoxyethylene nonylphenyl ether 0.5 Spices Appropriate amount antiseptic Appropriate amount Sea buckthorn leaf extract 0.02 Purified water To 100

Formulation Example 3: Cream

Ingredient name Example (% by weight) glycerin 5.0 stearic acid 8.0 squalane 5.0 Self-emulsifying glycerin monostearate 2.5 Polyoxyethylene sorbitan monostearate 1.5 propylene glycol 4.0 Stearyl glycyrrhetinate 0.2 vaseline 2.0 antioxidant Appropriate amount Spices dose antiseptic Appropriate amount Sea buckthorn leaf extract 0.02 Purified water To 100

Claims (7)

It is a composition for preventing and improving acne containing sea buckthorn extract as an active ingredient,
A composition for preventing and improving acne, wherein the sea buckthorn extract is one or more extracts selected from the group consisting of the stems, leaves and fruits of the sea buckthorn tree.
According to paragraph 1,
The sea buckthorn extract is a composition for preventing and improving acne, which is an extract extracted with water, an organic solvent, or a mixed solvent thereof.
According to paragraph 2,
The organic solvent is a composition for preventing and improving acne, wherein the organic solvent is at least one selected from the group consisting of alcohol having 1 to 4 carbon atoms, ethyl acetate, nucleic acid, and chloroform.
According to paragraph 1,
A composition for preventing and improving acne, wherein the sea buckthorn fruit extract is an oil extract obtained by concentrating the extract under reduced pressure with water, an organic solvent, or a mixed solvent thereof.
According to paragraph 1,
The composition for preventing and improving acne has antibacterial activity against Propionibacterium acnes.
According to paragraph 1,
A composition for preventing and improving acne, wherein the sea buckthorn extract is contained in an amount of 0.001 to 5.0% by weight based on the total weight of the composition.
According to paragraph 1,
The composition for preventing and improving acne has one or more formulations selected from the group consisting of skin, lotion, cream, and powder.