KR101840646B1 - Cosmetic composition comprising extract of Lotus corniculatus for improving acne - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving acne, comprising a bee yellow extract as an active ingredient.
According to the present invention, the bee yellow extract of the present invention has no side effects and is not only stable but also has excellent sterilizing action against Propionibacterium acnes as compared with the conventional ingredients and treatment methods for treating acne, Can be used as a cosmetic composition.

Description

[0001] The present invention relates to a cosmetic composition for extracting Lotus corniculatus for improving acne,

The present invention relates to a cosmetic composition for improving acne, and more specifically, to a cosmetic composition for improving acne containing an extract of bee yellow as an active ingredient.

Acne refers to inflammatory skin diseases that occur in hair follicles and sebaceous glands. The causes of such acne are largely caused by increased sebum secretion, follicular stenosis, and proliferation of acne bacteria (Propionibacterium acnes). Since sex hormones, especially male hormones, which are increased after puberty, excessively increase secretion of sebum, sebaceous lipid composition differs from skin epidermis and is easily oxidized and used as an energy source of bacteria. Therefore, Which can lead to acne. In addition, increased acne bacteria are recognized as antigens and inflammation reaction occurs. This causes papules and worsens, leading to destruction of the hair follicle wall and development of lesions such as cysts and pustules, where tissue is damaged.

In order to alleviate symptoms of acne, it is necessary to reduce sebaceous secretion, which is an energy source of acne, or to remove secreted sebum frequently. In addition, acne bacteria actively grow in anaerobic conditions, so if acne skin is removed well to prevent stenosis of the hair follicle, or the growth of acne bacteria is suppressed, acne symptoms may be alleviated.

 In order to inhibit acne bacteria, antibiotics such as tetracycline, minocycline, and erythromycin, external medicine such as benzoyl peroxide, anti-inflammatory drugs for treating inflammation, and hormone preparations for controlling sebum may be used.

In the field of cosmetics, methods such as resorcinol, sulfur, salicylic acid, and benzoyl peroxide were added to cosmetics to remove skin exfoliation and sterilization of acne bacteria. However, these ingredients are liable to cause adverse effects such as skin irritation and redness or dryness, and the effect of sterilization of acne bacteria is not so small because the concentration of cosmetic ingredients is limited by such side effects. Therefore, attempts to obtain acne improvement effect using natural and safe herbal medicine extracts have been continuing and so far there has been no satisfactory research result.

In recent years, studies have been actively conducted to search for effective ingredients that can reduce the above-mentioned adverse effects from natural resources such as plants and continuously use them, and have harmless effects on the human body but have strong anti-acne bacteria effect. In Korea, it is used as a raw material for functional cosmetics and soaps related to acne treatment and extracts of mushroom, green tea and golden, willow, Saururus chinensis, Licorice and Horsetail. It is progressing. However, the effect is very weak compared to conventional acne remedies, and it is difficult to mass-produce effective substances related to acne treatment.

Particularly, natural products are composed of various components in a complex manner. Therefore, the method for separating and purifying an active active substance differs depending on the physicochemical properties of the desired active substance, and effective methods for separation and purification based on various different principles Therefore, there are many difficulties in extracting and isolating a substance having anti-acne activity from natural products.

The scientific name of bee yellow is Lotus corniculatus var. It is japonicus . It is a perennial plant of a dicotyledonous plant rosemary bean, and it is also called a yellow stover. It grows in the shade of the mountains. It is about 30cm in height. In the base, there are many branches that grow diagonally or spread and have no hair. In Oriental medicine, its roots are called Baekmaegun. It stops the thirst, stops the thirst, stops the thirst, causes the cough, asthma, sputum, seawater and milk to come out well, choreography, obesity pain, eczema It has the effect of regulating the efficacy of removing and creating fever, pitting, hemorrhoids, hyaluronic acid, fever and hunger, cold, sore throat, colitis, blood stain and dysentery. There are many quotations in Chinese history rather than in Korea, and there is a record in <Tanghonggosa> that it is effective to stop the thirst, to quench thirst, to remove fever, to remove the thirst and to tribe.

However, only the above-described effect of the above-mentioned bee yellow is known, and in the conventional patent, the fact that ethanol and purified water mixed extract and hot water extract containing bee yellow are exhibited strong activity against acne bacterium, Therefore, the inventors of the present invention conducted a study on the prevention or treatment of acne in bee yellow, and as a result, confirmed the sterilizing action against Acne bacterium (Propionibacterium acnes).

 The present inventors have made intensive efforts to overcome the problems of the prior art. As a result, in order to develop a safe and effective cosmetic composition for acne, the present inventors investigated various natural products. Among them, the extracts of Propionibacterium acnes ), And thus completed the present invention.

Accordingly, it is a main object of the present invention to provide a cosmetic composition for improving acne, which comprises a bee yellow extract having an excellent sterilizing effect of Propionibacterium acnes, .

According to one aspect of the present invention, there is provided a cosmetic composition for improving acne, comprising a bee yellow extract as an active ingredient.

Conventionally, the ingredients used for improving acne in the cosmetics field have been used in cosmetics to remove skin exfoliation and acne bacteria, but since these ingredients stimulate the skin, side effects such as reddening of the skin and drying And there is a limit to the continuous use thereof on the skin. The inventors of the present invention confirmed that the extract of Bee Yellow Orchid, which is one of the natural plants continuously investigated by the present inventors, has excellent germicidal effect against acne bacteria and completed the present invention.

Lotus corniculatus is a perennial herb with rosemary roots of dicotyledonous plants and is also known as yellow stones. In Oriental medicine, its roots are called Baekmaegun. It is said to be effective in stopping thirst, releasing fever, causing coughing, asthma, seawater from fluids without sputum, and milk to come out well. However, as in the present invention, it is not known that it is effective to inhibit the growth of acne bacterium Propionibacterium acnes and to improve acne.

In the present invention, the bee yellow extract can be extracted with any extraction solvent conventionally known for extracting natural plants, and can be extracted with water, a lower alcohol having 1 to 4 carbon atoms, ether, ethyl acetate, chloroform, And water, and more preferably, ethanol is extracted.

In the present invention, the extract may contain 0.00001 to 80% by weight, preferably 0.0001 to 50% by weight, based on the total weight of the composition. If the amount is less than 0.0001% by weight, it may be difficult to improve the acne improvement effect. If the amount is more than 50.0% by weight, it may be difficult to improve the acne due to the increase of the content. Can be.

In the present invention, the extract is characterized by exhibiting an acne improvement effect by inhibiting the activity of Propionibacterium acnes.

According to the experimental example of the present invention, the antimicrobial activity against Propionibacterium acnes, an acne bacterium, was examined by a paper disk test. As a result, in case of the extract of bee yellow of the present invention, Salicylic acid (positive control) It was confirmed that it exhibited better antibacterial activity. From these results, it can be understood that the extract of the present invention is effective for improving acne by inhibiting Propionibacterium acnes, which is one of the acne bacteria, and is also suitable as a cosmetic composition for improving acne.

In the present invention, the composition may be in the form of a soft lotion, a convergent lotion, a lotion, a cream, a massage cream, an essence, an eye cream, an eye essence, a cleansing cream, a cleansing foam, a cleansing water, a pack, a powder, a body lotion, , A body essence, a makeup base, a foundation, a shampoo, a rinse and a body cleansing agent.

The composition of the present invention may contain other adjuvants in addition to the carrier, for example, preservatives, antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes.

INDUSTRIAL APPLICABILITY As described above, the composition of the present invention containing the bee yellow extract is suitable for commercialization as a cosmetic composition for improving acne because it exhibits an effect of improving acne by inhibiting acne bacterium (Propionibacterium acnes).

1 is a graph showing the results of a positive control group according to the antibacterial activity test of Example 2 of the present invention.
Fig. 2 is a graph showing the results of the bee yellower extract according to the antibacterial activity test of Example 2 of the present invention. Fig.
Fig. 3 is a diagram showing the results of the bee yellow extract according to the cytotoxicity evaluation test of Example 2 of the present invention. Fig.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1: Collection and preparation of materials

1. Collection and preparation of materials

The bee used in the test was used in May 2013, which was supplied to Oriental Medical Clinic from China. It was supplied by Tongyang Hub Co., Ltd.

2. Preparation of 70% ethanol extract of bee yellow

For the preparation of 70% ethanol extract of bee yellow, 1,000 mL of ethanol was added to 100 g of dried beeswax yellow powder, and the mixture was extracted at room temperature for 72 hours, filtered with a 185 mm (wattman) filter, After concentration, the solid was extracted. The obtained extract is 6.8 g.

Experimental Example 2: Cytotoxicity test

The presence or absence of cell proliferation promoting activity in the human fibroblast Hs68 fibroblast was assayed using MTT (3- (4,5-dimethylthiazol-2yl) -2,5-diphenyltetrazolium bromide) reagent.

Human fibroblast Hs68 fibroblast was dispensed into 96-well plates at a concentration of 2 × 10 ⁴ cells / well and cultured in an incubator at 37 ° C and 5% CO ² for 24 hours. The medium was removed and the concentration was adjusted to 0.1 μg / ml, 0.5 μg / ml, 1 μg / ml, 5 μg / ml, 10 μg / ml, 50 μg / ml and 100 μg / ml, 500 μg / ml and 1000 μg / The extract was treated with the medium and cultured at 37 ° C in 5% CO _{2 for} 24 hours. To confirm the cytotoxic effect, 20 μl of MTT (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide) dissolved in DPBS was added and reacted. MTT changes to dark blue by the respiratory chain enzyme in the mitochondria of living cells as a light yellow substrate. After 4 hours, the reaction solution was discarded and DMSO was added. The absorbance at 570 nm was measured with a microplate reader. Cell viability was expressed as a percentage of the average absorbance value of the control group without any sample.

 As shown in Fig. 3, there is no decrease in cells due to the bee yellow extract. Therefore, it was confirmed through this experiment that the bee yellow extract was not cytotoxic.

Example  3: Propionibacterium  Test of antimicrobial activity against acnes

The antimicrobial activity against propionibacterium acnes was measured using the agar diffusion method for the solid content of the bee yellow obtained by the above method.

I. Experimental Materials

Propionibacterium acnes, paper disc (10 mm), Brain Heart Infusionagar, tweezers, sterile distilled water, ethanol

The Propionibacterium acnes 3314 strain used in this study was purchased from the BRC, Korea Research Institute of Bioscience and Biotechnology.

II. Experimental Method

1) prepare an agar plate; Sterilize the Brain Heart Infusion agar and dispense 25 ml per plate.

2) strain smearing; The number of bacteria in each strain is adjusted to 10 ⁵ , and 100 μl is plated.

3) loading of raw material into disc (10mm); After mixing the extract with 1%, 0.5%, and 0.1% of the bee yellow which is dissolved in 10% ethanol, add 50 μl of the extract to a disc (paper paper, Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan).

4) positive control loading on disc (10mm); Salicylic acid was used as a positive control. Salicylic acid dissolved in 10% ethanol was adjusted to 0% and 1.0%, and 50 μl of the solution was loaded on a disc (Advantec Filter Paper, Toyo Roshi Kaisha Ltd., Japan).

5) planting disc; Pick up the discs loaded with sterilized tweezers and plant them on the smear plate.

6) culture; Incubate the plates in an anaerobic jar to block oxygen and incubate at 37 ° C.

7) The size (in mm) of the circular growth zone formed around the paper disk was measured, and all the tests were independently repeated three times to obtain the average value.

The results are shown in Table 1 and FIGS. 1 and 2.

The positive control (Salicylic Acid) Bee yellow density(%) Width (mm) density(%) Width (mm) 0 0 0 0 0 0 0.1 4.5 4.0 4.6 1.0 7.5 0.5 7.7 7.2 7.6 1.0 10.1 10.2 10.3

As a result, as shown in Table 1, it can be seen that the area of the clean zone of the bee yellow extract increases in a concentration-dependent manner. In the case of bee yellow, the mean value of about 4.4mm for 0.1%, the mean value of about 7.5mm for 0.5%, and the average value of about 10.2mm for 1.0%.

In addition, in FIG. 1, which is an evaluation result of antimicrobial activity using Salicylic Acid as a positive control against Propionibacterium acnes, it was confirmed that a clean zone of about 7.5 mm was formed when 1.0% of salicylic acid was used 2, which is a result of evaluating the antimicrobial activity of the extract of the bee yellow on Propionibacterium acnes, showed that the width of the clean zone was increased in a concentration dependent manner. When the concentration of 1.0% It was confirmed that a clean zone was formed at about 10.1 mm, which is wider than the positive control group.

From the above results, it was confirmed that the bee yellow extract had an excellent antimicrobial activity against Propionibacterium acnes.

Test Example 4: Skin irritation experiment

Most of herbal extracts have less skin irritation than general chemical products. However, since there is a problem in that skin harmful substances can not be properly removed, skin troubles can be caused. Therefore, the degree of skin irritation of the cosmetic composition of the present invention is measured A patch test was conducted on 31 subjects.

I. Test Overview

Twenty - three lesions of average age 26.2 years old and 8 males were exposed to patches for 24 hours, and skin irritation was observed 30 minutes, 24 hours, and 48 hours after patches were applied.

(31 out of 32 cases were excluded, except for the results of one study subject estimated to be Angry back reaction.)

II. Test Methods

1) Application method: Closed patch test

2) Application amount: 20 / / chamber (IQ chamber)

3) Number and duration of application: 24 hours after the removal of the patches and patches, and 30 minutes and 24 hours after the removal of the patches.

4) Reaction test: 30 minutes, 24 hours and 48 hours after the removal of the patches, according to the terminology suggested in the ICDRG guideline

5) Acceptance criteria: Observations were made at 30 minutes, 24 hours, and 48 hours after removal of the patch, and skin reactions were assessed according to the following criteria, which were based on ICDRG guideline (Table 2).

6) Result calculation method:

30, 24, and 48 hours were calculated using Equation 1 below, and the results were determined according to the criteria given in Table 3 for the average degree of reactivity for each substance. The results are also shown in Table 4.

Symbol (grade) Clinical observation -(0)
± (0.5)
+ (1)
++ (2)
+++ (3) None
Doubtful reaction; faint erythema only
Weak (non-vesicular) reaction; erythema, slight infiltration
Extreme (bullous or ulcerative)
Intense erythema with edema & vesicles

[Formula 1]

Rnage of Mean Score Criteria 0.00? R <1.01 Low stimulus 1.01? R <2.00 Light stimulus 2.01? R <3.50 Moderate irritation 3.51? R River stimulation

Test substance
30 mins
24 hrs
48 hrs Mean
Score
(n = 31) Judgment
(Grade) - ± + ++ +++ - ± + ++ +++ - ± + ++ +++ Bee Yellow (1.0%) One 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 Low stimulus

Test Example 5: Acne improvement effect panel test

In order to determine the anti-inflammatory effect of the bee yellow extract of Example 1 on Propionibacterium acnes, the extract of Example 1 was used and the external preparation was formulated into a nutritional cream according to the composition ratio shown in Table 5 below. To prepare Formulation Example 1 and Comparative Formulation Example 1, respectively. The unit of the content ratio of the following ingredients is% by weight.

Compounding ingredient Formulation Example 1 Comparative Formulation Example 1 Purified water to 100 to 100 Example 1 1.0 - Sitosterol 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 Setares-4 1.2 1.2 cholesterol 1.5 1.5 Dicetyl phosphate 0.4 0.4 Concentrated glycerin 5.0 5.0 Sunflower oil 10.0 10.0 Carboxyvinyl polymer 0.2 0.2 Xanthan gum 0.3 0.3 Triethanolamine 0.2 0.2 Spices Suitable amount Suitable amount antiseptic Suitable amount Suitable amount

In order to confirm the anti-inflammatory effect of Propionibacterium acnes of Formulation Example 1 and Comparative Formulation Example 1 in Table 5, evaluation was made as follows. 40 men and women in their 30s and 40s with acne were divided into two groups of 20 persons for each of Formulation Example 1 and Comparative Formulation Example 1 to apply the nutritional cream twice daily for 4 weeks to the face. The effect of improving acne was visually evaluated before application and after application (after one week, two weeks, and four weeks had elapsed). In the visual evaluation, when the degree of acne acne was improved by 70% or more compared with that before the experiment, the acne improvement effect was excellent (⊚), and when the degree of acne acne was improved from 40% or more to less than 70% (△), the degree of acne acne was less than 10%, or it was not improved at all, or even worse And the acne improvement effect was evaluated as poor (x). The results are shown in Table 6.

Acne improvement Formulation Example 1 Comparative Formulation Example 1 1 week 2 weeks 4 weeks 1 week 2 weeks 4 weeks ◎ 3 5 7 0 One 0 ● 7 5 6 One One One △ 7 8 5 6 5 5 × 3 2 2 13 13 14

As a result, as shown in Table 6, the cosmetic composition of Formulation Example 1 containing bee yellow was more excellent in improving acne than the cosmetic composition of Comparative Formulation Example 1 which did not contain any bee yellow. there was.

Claims (5)

A cosmetic composition for improving acne characterized by containing an extract of Bee Yellow as an active ingredient and the extract of Bee Yellow which inhibits the activity of Propionibacterium acnes.
[Claim 2] The cosmetic composition for improving acne according to claim 1, wherein the bee yellow extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water.
The cosmetic composition for improving acne according to claim 1, wherein the extract comprises 0.0001 to 50% by weight based on the total weight of the composition.
delete The composition according to claim 1, wherein the composition is selected from the group consisting of a soft lotion, a convergent lotion, a lotion, a cream, a massage cream, an essence, an eye cream, an eye essence, a cleansing cream, a cleansing foam, a cleansing water, a pack, a powder, a body lotion, Wherein the composition has one formulation selected from the group consisting of an oil, a body essence, a makeup base, a foundation, a shampoo, a rinse, and a body cleanser.

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