KR101878069B1 - Composition comprising herbal mixture extracts and hinoki oil for preventing or treating acne and atopic dermatitis - Google Patents

Abstract

The present invention relates to a composition for preventing or treating acne or atopic dermatitis containing an extract of a herbal medicine mixture containing gold, yellowtail, yellow whites and gardenia, a cosmetic composition having skin moisturizing, acne, atopic dermatitis, . The composition comprising the mixture of the herbal mixture extract and the whitening oil has a synergistic effect on inhibition of growth of Propionibacterium acnes and Staphylococcus aureus which are causative agents of acne and atopy And can be easily used as various types of pharmaceutical composition, external preparation for skin, and cosmetic product.

Description

[0001] The present invention relates to a composition for preventing or treating acne or atopic dermatitis containing an extract of a herbal mixture and an opacifying oil, and to a method for preventing or treating acne or atopic dermatitis,

The present invention relates to a composition for the prophylaxis or treatment of acne or atopic dermatitis containing an extract of a herbal medicine mixture containing golden, yellowtail, yellowtail, and germanium and a whitish oil.

BACKGROUND ART [0002] In the human body, skin functions as a protective film for protecting a living body from the external environment, and serves to prevent the loss of biological components in the human body, that is, water and electrolytes, and to prevent harmful substances from entering the human body from the outside . The skin is divided into the skin layer, the dermal layer and the subcutaneous fat layer. The epidermal layer consists of keratinocytes and melanocytes. Since the keratinocytes in the outermost layer of the skin are in direct contact with the external environment, they are required to have strong resistance against physical, chemical, or material permeation and prevent moisture from being lost to the outside At the same time as zooming, it should maintain its flexibility by holding proper water on its own.

Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer, which is about 10 to 30% in the keratin layer. The water supplied from the dermis layer mainly migrates to the upper part of the stratum corneum by passive diffusion and eventually to the outside. This is called Trans Epidermal Water Loss (TEWL). It is known that the lipid component of the keratinocyte layer and epidermal lipid, which maintains the transdermal water loss at an appropriate level, is the lipid component. In the keratinocyte layer, a substance having hydrophilic water retention ability called "Natural Moisturizing Factor" (NMF) is present and is known to play an important role in moisturizing the skin. When the normal keratinocyte layer is maintained at a moisture content of 10 to 30%, the skin is smooth and soft, and the body protection functions normally.

However, when the moisture content of keratinocytes is less than 10%, the skin becomes rough and loses protective function of the body and aging occurs. For example, in the case of dry skin, the keratinocyte aggregation is weakened and scaling phenomenon appears in which the keratinocyte is peeled off like a scaly piece from the skin surface. Thus, the dryness of the skin is caused by the moisture content Is less than normal skin. In addition to this, even healthy skin may be in a harsh environment, such as wind, cold weather, sunshine, cleansing, shaving, etc., which causes water shortage, which can cause a bad skin condition.

Therefore, it is very important to keep the moisture content of keratinocytes in skin properly. To this end, cosmetics were added with ingredients similar to sebum, NMF ingredients, or moisturizers such as polyols. For example, glycerin, solitol and the like having three or more hydroxyl groups (OH groups) as water-soluble polyols exhibit excellent moisture resistance, but they are very sticky, which makes them feel uncomfortable when used. Propylene glycol having two hydroxyl groups, 1,3- Glycol also causes side effects of the skin. In addition, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, urea and the like have a problem of deteriorating the emulsification stability of cosmetics due to their high electrolytic property. Also, amino acids, collagen, There is a limit to its ability to moisturize, though it is capable. The ability to retain the moisture of the stratum corneum is controlled by the natural moisturizing factor (NMF) composed of sebum component, amino acid, lactic acid, urea and inorganic salt, and is safe and usable as a natural moisturizing factor in the external preparation for skin, The development of high-quality materials is required.

On the other hand, skin damaged by keratin is also associated with the cause of atopy and acne. The causes of acne are still not clearly defined, but if genetic and environmental factors are excluded, three important causes are directly related to the occurrence of acne. have. The three most important causes of acne are described below. The first cause is excessive sebum secretion. The formation of sebum is accomplished by the action of androgenic hormones. Through the abnormal increase of this male hormone or various related mechanisms, the sebaceous glands become hyperfunction and result in excess sebum. The second is explained by the closure of the pores due to hyperkeratinization of the hair follicles. The resulting sebum must be smoothly drained out of the skin, but due to the overgrowth of the hair follicles, the pores are clogged and smooth release of the sebum is suppressed, resulting in stagnation of sebum in the hair follicle resulting in formation of fine sebaceous glands. In association with sebaceous glands, there is all the enzymes necessary to convert an inactive steroid precursor into active androgen in the skin, and 5α-reductase is one of them. Testosterone, one of the male hormones, enters the sebaceous gland and is converted to dihydrotestosterone by the 5α-reductase present in the cell (sebocyte). It is used as a signal to express a multistep pathway such as gene expression And as a result, sebum is produced. The third cause is caused by skin infections such as Staphylococcus epidermis , Staphylococcus aureus , and Propionibacterium acnes , which cause acne. This causes acne lesion worsening As a major cause of The acne bacteria that reside in the hair follicles form pore wall irritants. As the excess sebum secretion continues, the size of the fine cottonseed is gradually increased and the cottonseed is developed. As a result, the hair follicle wall becomes thinner, and the irritant material produced by the acne bacteria stimulates the thinned hair follicle wall, (Papule → pustule → nodule → scar). Various studies have been conducted on methods for treating or improving acne by focusing on sebum suppression and antimicrobial effect against skin diseases according to the cause of such acne.

Although the pathogenesis of atopic dermatitis has not yet been fully elucidated, it has been known that atopic dermatitis is caused by a chronic inflammatory reaction caused by an irritant immune reaction (allergic reaction) to a common substance (allergen) in the environment. In addition, atopic dermatitis is a chronic inflammatory disease that occurs before the development of asthma and allergic diseases, and the symptoms vary depending on the concentration of chemokine, the quantitative change, and the degree of activity (Curr. Allergy Asthma Rep., 2005, 5, 284-290), and also refers to a phenomenon in which a Th2-type lymphocyte invades a lesional skin.

It is known that 0.5 ~ 1% of the world population, especially 5 ~ 10% of children, suffer from atopic dermatitis. Environmental factors are known to be involved in the onset and deterioration. As an example, the incidence of atopic dermatitis is correlated with the amount of exposure to house dust mites, and it is known that the severity of atopic dermatitis also has a significant correlation. It is also known to reduce the severity of atopic dermatitis by reducing exposure to house dust mites in the environment. In addition, atopic dermatitis appears to be caused by a combination of dry skin, easily itchy skin, bacterial / viral / fungal infection, emotional and environmental factors. In recent decades, the incidence of atopic dermatitis has been rising, and the number of patients in Korea is also increasing.

IL-8 (interleukin-8), one of the cytokines that cause atopic dermatitis, is an important inflammatory chemokine secreted from the airway epithelium. It is mainly expressed at the early stage of inflammation. IL-8 induces bronchial hyperresponsiveness. And bronchial asthma. As an example of this, treatment of THP-1 cells or EoL-1 cells with an extract of Leptospira, which causes atopic dermatitis, increases the amount of IL-8, a cytokine that causes atopic dermatitis.

Another type of cytokine that causes atopic dermatitis is MCP-1 (monocyte chemoattractant protein-1), which binds to CCR2 (chemokine receptor) and MCP- Have been observed to be impaired in resistance to certain bacterial infections and have been reported to be similar to those observed in animals with the CCR2 gene removed. In addition, MCP-1 converts Th0 cells into cells that secrete Th2 cytokines in the test tube, and when intravenously injected with MCP-1, the production of IL-12 is decreased and the production of IL-4 is increased have. In addition, there have been reports that skin damage caused by Staphylococcus aureus is also a source of atopy.

The most widely used therapeutic agents for atopic dermatitis are drugs such as steroids, antihistamines, and antibiotics. Among these, steroids are the most widely used therapeutic agents for inflammatory diseases such as bronchial asthma, but the mechanism by which steroids respond in the body is still unknown. Some patients, such as bronchial asthma and rheumatoid arthritis, show steroid resistance that does not respond to steroids. In addition, atopic dermatitis is often chronicized rather than completely treated with a steroid-based drug, resulting in side effects such as denaturation and fibrosis of the tissue. When resistance to steroids increases, various cytokines such as TGF-beta, IL-4 and IL-13 associated with fibrosis are secreted through various cells in the chronic inflammatory reaction, the secretion of IL-6, which activates fibroblast, is increased, resulting in the proliferation of many fibroblasts and the over-production of extra cellular matrix, resulting in cell and tissue transformation and fibrosis.

Therefore, therapeutic agents for atopic dermatitis such as steroid agents are gradually restricted due to various side effects as described above, and a composition for treating a new concept of atopic dermatitis which is effective for atopic dermatitis but has no side effects is required have.

Scutellaria baicalensis roots (Scutellariae Radix) used as herbal medicine are used as herbs, diuretics, branching agents, fungicides and anti-inflammatory agents in Oriental medicine.

Plants such as Plagiorhegma dubium Maxim., Coptis chinensis Franch., Coptis japonica Makino, Coptis teeta , and Coptis sinensis are used as the antler . In addition, foreign plants of Coptis are used as antler . Chrysanthemum has been reported to have the ability to lower fever, remove moisture and detoxify, and exhibit the virulence of white candida and a series of dermatophytes. My heart aches and I can not sleep. I sweat with mild diarrhea, dysentery, and stomach pains. I have swollen eyes because of liver disease. I have been sore. I have been used to bleed blood from stomatitis, stomatitis and hemorrhoids.

Hwang Baek is a dried peel of Phellodendron amurense Rupr. It has the function of releasing fever , damping and deciphering . It has blood pressure lowering action, blood coagulation promoting action, mating function, uterine contraction, It has been shown that it exhibits antimicrobial action and anti-inflammatory action against a series of pathogenic microorganisms. It is used for diarrhea, diarrhea, jaundice, ooze, fever, pulmonary tuberculosis, oil wells, itching, boils, eczema, bacterial rash, inflammation of the urinary bladder and urethra, and osteoarthritis of the osteotomy.

The gardenia is the dried fruit of the gardenia jasminoides Ellis, the madder plant. Gardenia is planted in the central part of Korea and in the greenhouse in the north. It is dried in the sun after the ripe fruit in autumn. The taste is good and the quality is cold. It affects the heart, menopause, stomach, liver, and trichomes. It lowers the heat, heals the chest tightly, eliminates the heat, and makes the urine come out well. It also stops the bleeding and sinks the bookkeeping and decodes.

Phytoncide is a natural antimicrobial substance that trees excrete to protect themselves from pests and germs. It has the effect of relieving stress, strengthening cardiopulmonary function, sterilizing action, and inhibiting the reproduction of house dust mite causing atopy. Chamaecyparis obtusa ) contains a lot of phytoncide in particular, and there is a lot of use of the wood for various construction materials, bedding, and kitchen utensils. Since hinoki oil, which is an essential oil extracted from cottonwood, contains such a large amount of phytoncide, it is used in various applications such as antibacterial, anti-inflammation, and atopic improvement. Cotton white oil refers to a product obtained by separating essential oils contained in cottonwood by steam distillation.

The inventors of the present invention have conducted studies on the physiological activities of herbal medicines such as golden, yellowtail, yellowtail, gardenia, whitish, etc., and found that a composition containing a herbal medicine mixture containing these gold, , And it was confirmed that there is an effect of inhibiting atopic dermatitis and an effect of improving skin's horny quality due to moisturizing and the like, thereby completing the present invention.

Korean Patent Laid-Open No. 10-2009-0038648 (title of the invention: Cosmetic composition for acne skin containing white cotton oil, Applicant: JGJ, Publication date: Apr. 21, 2009) Korean Patent Laid-Open No. 10-2014-0070946 (entitled "Herbal Mixed Fermentation Extract and Pharmaceutical Composition Containing it as an Active Ingredient, Applicant: Shim Kyu Chul, Disclosed: 2014.06.11) Korean Patent No. 10-1081914 (Name of invention: composition for external application for skin containing extract extracted from crude drug mixture, applicant: Hanbul Cosmetics Co., Ltd., registered on November 11, 2011)

It is an object of the present invention to provide a composition for preventing or treating acne or atopic dermatitis containing an extract of a herbal medicine mixture containing gold,

The present invention relates to a composition for the prophylaxis or treatment of acne or atopic dermatitis containing an extract of a herbal medicine mixture containing golden, yellowtail, yellowtail, and germanium and a whitish oil.

50 to 200 parts by weight of cordierite, 50 to 200 parts by weight of yellowish white, and 50 to 200 parts by weight of gardenia may be contained in the crude drug mixture, based on 100 parts by weight of gold.

The extract of the crude drug mixture can be extracted by using a 1,3-butylene glycol aqueous solution as a solvent.

The composition may contain an extract of the crude drug mixture and a whitening oil in a weight ratio of 10: 0.1-5.

The extract and the whitening oil of the herbal medicine mixture including the golden, yellowtail, yellowtail, and the germanium are added to at least one strain selected from the group consisting of Propionibacterium acnes and Staphylococcus aureus , .

The present invention also relates to a cosmetic composition comprising an extract of a herbal medicine mixture comprising the above-mentioned golden, yellow, white and germanium, and a simplex oil. The cosmetic composition is characterized in that it has skin moisturizing, acne, atopic dermatitis, or skin alleviating effect.

Accordingly, the present invention can provide a cosmetic composition containing the cosmetic composition as a raw material or an effective ingredient.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a composition for the prophylaxis or treatment of acne or atopic dermatitis containing an extract of a herbal medicine mixture containing golden, yellowtail, yellowtail, and germanium and a whitish oil.

The extract of the crude drug mixture can be extracted with a 1,3-butylene glycol aqueous solution as a solvent, and the 1,3-butylene glycol aqueous solution is preferably 20-50% (v / v) Butylene glycol aqueous solution, and most preferably 30 to 40% (v / v) 1,3-butylene glycol aqueous solution. The extract of the herbal medicine mixture extracted using the solvent of this condition is superior to the solvent extract extracted with the C1-C4 alcohol or the mixed solution thereof. The C1 to C4 alcohols may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol.

The aqueous 1,3-butylene glycol solution used in the preparation of the crude drug mixture extract may be used in an amount of 1 to 40 times (1 to 40 l based on 1 kg), preferably 5 to 40 times Can be used. The extraction conditions of the crude drug mixture may be from 20 to 50 DEG C for 1 minute to 48 hours. The above process can be repeated 1 to 4 times.

The extract of the crude drug mixture and the whitening oil may be contained at a weight ratio of 10: 0.1-5. In the case of the composition not mixed at this ratio, the acne improvement effect may be poor or the acne may be even worse.

As the extraction apparatus used in the extraction process of the present invention, a conventional extraction apparatus and an ultrasonic pulverization extractor may be used. The extract of the crude drug mixture thus prepared can be removed by hot air drying, vacuum drying or freeze drying.

The extracts of the herbal medicine mixture including the golden, yellowtail, yellowtail, and germanium and the whitening oil have an antibacterial effect on Propionibacterium acnes , so that they can be effectively used for preventing, improving or treating acne.

The present invention can provide a pharmaceutical composition or a skin external preparation for preventing or treating acne or atopic dermatitis containing an extract of a herbal medicine mixture including gold, yellowtail, yellowtail, and germanium, a white oil and a pharmaceutical excipient. At this time, the pharmaceutical composition or the external preparation for skin may be administered to mammals such as rats, livestock, and humans in various routes. The pharmaceutical composition may be administered orally or vascularly. Preferably, the pharmaceutical composition may be administered orally or parenterally such as a spray, a liquid preparation, a lotion, a foaming agent, a tape agent, an aerosol agent, an external preparation, a gel, a cream, , A patch, a mask pack or the like. The pharmaceutical composition or the external preparation for skin may contain 0.001 to 99.9% of the extract of the herbal medicine mixture and the whitening oil. The ingredients other than the active ingredient of the pharmaceutical composition or external preparation for skin of the present invention may be any ingredient commonly used in external preparations applied to the skin. For example, bases such as white petrolatum (vaseline), yellow petrolatum, lanolin, purified beeswax, cetanol, stearyl alcohol, stearic acid, hydrogenated oil, hydrocarbon gel, polyethylene glycol, liquid paraffin and squalane; Oleic acid, isopropyl myristate, glycerol triisooctanoate, crotamitone, diethyl sebacate, diisopropyl sebacate, diisopropyl adipate, hexyllaurate, fatty acid, fatty acid ester, vegetable oil, Solvents or stabilizers such as alcohols; Tocopherol derivatives, antioxidants such as L-ascorbic acid, dibutylhydroxytoluene and butylhydroxyanisole; preservatives such as p-hydroxybenzoate; Curling agents such as glycerin, flufolene glycol and sodium hyaluronate; Surfactants such as polyoxyethylene derivatives, glycerol fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters and lecithin; Thickeners such as carboxyvinyl polymer, xanthan gum, carboxymethylcellulose and sodium carboxymethylcellulose, hydroxypropylcellulose and hydroxypropylmethylcellulose; Stabilizers; Preservatives; Adsorption promoters; And other suitable fillers may be added. The pharmaceutical composition or external preparation for skin of the present invention may be prepared by mixing well the components well known in the art for preparing external preparations, together with a suitable base, if necessary, and the preparation thus prepared is applied to lesions as required .

The dosage of the pharmaceutical composition or external preparation for skin of the present invention will vary depending on the age, sex, body weight of the subject to be treated, and the severity of the pathological condition, the disease or pathological condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The present invention also relates to a cosmetic composition comprising an extract of a herbal medicine mixture comprising the above-mentioned golden, yellow, white and germanium, and a simplex oil. The cosmetic composition is characterized in that it has skin moisturizing, acne, atopic dermatitis, or skin alleviating effect. Accordingly, the present invention can provide a cosmetic composition containing the cosmetic composition as a raw material or an effective ingredient. The formulations of the cosmetics may be prepared in any cosmetic formulations conventionally produced in the art and may be formulated into ointments, essences, whitening creams, lotions, emulsions, packs, general lotions, skin milks, skins, creams, Soap, softening lotion, medicated lotion, whole body cleanser, cleansing oil, cleansing cream, cleansing tissue and cleansing water.

The cosmetic preparation of the present invention may contain an ingredient selected from the group consisting of animal oils, vegetable oils, waxes, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, lactose, But are not limited to, calcium silicate, polyamide powder, chlorofluorohydrocarbons, propane / butane or dimethyl ether, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propyleneglycol, Glycerol aliphatic esters, polyethylene glycols or sorbitan fatty acid esters, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, Aga or Trakant, Province But are not limited to, alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide ether sulfates, alkylamidobetaines, fatty acid glycerides, fatty acid diethanolamides, Lanolin derivatives or ethoxylated glycerol fatty acid esters.

The present invention relates to a composition for preventing or treating acne or atopic dermatitis containing an extract of a herbal medicine mixture containing gold, yellowtail, yellow whites and gardenia, a cosmetic composition having skin moisturizing, acne, atopic dermatitis, . The composition comprising the mixture of the herbal mixture extract and the whitening oil has a synergistic effect on inhibition of growth of Propionibacterium acnes and Staphylococcus aureus which are causative agents of acne and atopy And can be easily used as various types of pharmaceutical composition, external preparation for skin, and cosmetic product. Korean Patent Publication No. 10-2009-0038648, Korean Patent Laid-Open No. 10-2014-0070946, Korean Patent No. 10-1081914, and the like, a cosmetic composition containing raw material herbal medicine, There has been no disclosure of a composition such as a cosmetic preparation or a skin external preparation prepared by using the synergistic effect of an extract of a crude drug mixture and a whitening oil.

Fig. 1 shows the skin condition of a person who used the soap of the cosmetic preparation example 1-1 of the present invention for 1 month.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

≪ Example 1: Preparation of an extract of crude drug mixture >

(V / v) 1,3-butylene glycoll aqueous solution was added to the herbal mixture mixed with the contents as shown in Table 1 for 2 hours at room temperature After that, the medicinal herb was filtered out and the remaining liquid phase was concentrated under reduced pressure to obtain an extract of the herbal mixture.

Condition Golden (g) Chrysanthemum (g) Yellow (g) Gardener (g) Example 1-1 100 100 100 100 Examples 1-2 100 150 50 100 Example 1-3 100 200 50 50 Examples 1-4 100 50 200 50 Examples 1-5 100 50 50 200

< Example  2. Mixture of crude drug mixture and linseed oil>

The extract of the crude drug mixture prepared in Example 1 and the whitening oil were mixed in the weight ratios shown in Table 2 below.

Condition The extract (g) of Example 1-1 The extract (g) of Example 1-2 The extract of Example 1-3 (g) The extract (g) of Example 1-4 The extract (g) of Example 1-5 Cotton oil (g) Example 2-1 10 0 0 0 0 One Example 2-2 10 0 0 0 0 5 Example 2-3 0 10 0 0 0 One Examples 2-4 0 0 10 0 0 One Example 2-5 0 0 0 10 0 One Examples 2-6 0 0 0 0 10 One

&Lt; Comparative Example 1 > Preparation of comparative herbal mixture extracts &gt;

Comparative Examples 1-1 to 1-8. Extraction of aqueous butylene glycol solution

An extract of the crude drug mixture was prepared in the same manner as in Example 1, and the mixing ratios of the herbal medicines were listed in Table 3 below.

Condition Golden (g) Chrysanthemum (g) Yellow (g) Gardener (g) Comparative Example 1-1 100 10 280 10 Comparative Example 1-2 0 130 130 140 Comparative Example 1-3 200 0 100 100 Comparative Example 1-4 0 200 0 200 Comparative Example 1-5 400 0 0 0 Comparative Example 1-6 0 400 0 0 Comparative Example 1-7 0 0 400 0 Comparative Example 1-8 0 0 0 400

Comparative Example 1-9. Ethanol extraction

An extract of the crude drug mixture was prepared in the same manner as in Example 1-1, except that ethanol was used as a solvent.

Comparative Example 1-10. Ethanol aqueous solution extraction

An extract of the crude drug mixture was prepared in the same manner as in Example 1-1 except that a 70% (v / v) aqueous ethanol solution was used as a solvent.

Comparative Example 1-11. Water extraction

An extract of the crude drug mixture was prepared in the same manner as in Example 1-1 except that water was used as the solvent and the extraction temperature was maintained at 90 ° C and freeze-dried instead of concentration under reduced pressure.

&Lt; Comparative Example 2 > Mixing of Comparative Herbal Medicine Mixture with Cotton White Oil &gt;

The herbal mixture and the whitening oil were mixed in the same manner as in Example 2, and the mixing ratios at the respective conditions were listed in Table 4 below.

Condition The extract of Example 1-1 The extract of Comparative Example 1-1
(g) The extract of Comparative Example 1-2
(g) The extract of Comparative Example 1-3
(g) The extract of Comparative Example 1-4
(g) The extract of Comparative Example 1-5
(g) The extract of Comparative Example 1-6
(g) The extract of Comparative Example 1-7
(g) The extract of Comparative Example 1-8
(g) The extract of Comparative Example 1-9
(g) The extract of Comparative Example 1-10
(g) The extract of Comparative Example 1-11
(g) Linseed oil
(g) Comparative Example 2-1 One 0 0 0 0 0 0 0 0 0 0 0 10 Comparative Example 2-2 5 0 0 0 0 0 0 0 0 0 0 0 10 Comparative Example 2-3 0 10 0 0 0 0 0 0 0 0 0 0 One Comparative Example 2-4 0 0 10 0 0 0 0 0 0 0 0 0 One Comparative Example 2-5 0 0 0 10 0 0 0 0 0 0 0 0 One Comparative Example 2-6 0 0 0 0 10 0 0 0 0 0 0 0 One Comparative Example 2-7 0 0 0 0 0 10 0 0 0 0 0 0 One Comparative Example 2-8 0 0 0 0 0 0 10 0 0 0 0 0 One Comparative Example 2-9 0 0 0 0 0 0 0 10 0 0 0 0 One Comparative Example 2-10 0 0 0 0 0 0 0 0 10 0 0 0 One Comparative Example 2-11 0 0 0 0 0 0 0 0 0 10 0 0 One Comparative Examples 2-12 0 0 0 0 0 0 0 0 0 0 10 0 One Comparative Example 2-13 0 0 0 0 0 0 0 0 0 0 0 10 One

&Lt; Preparation of cosmetic preparation 1. Preparation of soap >

2 wt% of olive oil, 5 wt% of palm oil, 20 wt% of sorbitol, 0.2 wt% of carbomer, 0.5 wt% of hydrogenated castor oil, 10 wt% of dipropylene glycol, 0.15 wt% of phenoxyethanol, An emulsion containing 0.02 wt.% Of acetic acid, 69.13 wt.% Of water, 1 wt.% Of each composition of Example 2, and 0.2 wt.% Of triethanolamine was prepared and mixed with 10 g of the emulsion and 90 g of a natural soap base 5 &lt; / RTI &gt;

Soap condition Composition contained in soap Cosmetic Preparation Example 1-1 Example 2-1 Cosmetic Preparation Example 1-2 Example 2-2 Cosmetic Preparation Example 1-3 Example 2-3 Cosmetic Preparation Examples 1-4 Examples 2-4 Cosmetic Preparation Examples 1-5 Example 2-5 Cosmetic Preparation Example 1-6 Examples 2-6

&Lt; Preparation of cosmetic preparation 2. Preparation of skin >

5.0 wt% of the composition of Example 2-1, 5.2 wt% of propylene glycol, 1.5 wt% of oleyl alcohol, 3.2 wt% of ethanol, 3.2 wt% of polysorbate 20, 2.0 wt% of benzophenone- By weight,% by weight of glycerin, 3.5% by weight of glycerin, the amount of preservative, and the amount of purified water remaining, was prepared using a conventional method.

&Lt; Cosmetic preparation example 3. Lotion production >

5.0 wt% of the composition of Example 2-1, 1.0 wt% of cetostearyl alcohol, 0.8 wt% of glyceryl monostearate, 0.3 wt% of sorbitan monostearate, 1.0 wt% of polysorbate 60, 5.0 wt% of mineral oil, , Cyclomethicone 3.0% by weight, dimethicone 0.5% by weight, allantoin 0.1% by weight, glycerin 5.0% by weight, alcohol 2% by weight, propylene glycol 3.0% by weight, flavorant amount, preservative amount, To prepare a lotion.

&Lt; Cosmetic preparation example 4. Essence preparation >

The composition of Example 2-1, 5.0 wt% of propylene glycol, 3.0 wt% of glycerin, 0.5 wt% of allantoin, 0.01 wt% of EDTA-2Na, 5.0 wt% of ethanol, 1.5 wt% of triethanolamine, 2.0 wt% of squalane, The essence was prepared by a conventional method using 2.5% by weight of beeswax, 60% by weight of polysorbate, 1.0% by weight of carboxyl vinyl polymer, 2.5% by weight of sorbitan sesquioleate, the amount of flavor, the amount of preservative, and the amount of purified water .

&Lt; Preparation of cosmetic preparation 5. Cream production >

10.0% of the composition of Example 2-1, 0.7% of polyoxyethylene sorbitan monostearate, 0.5% of sorbitan sesquioleate, 0.6% of cetyl alcohol, 0.75% of stearic acid, 0.6% of glyceryl monostearate, 15.0% , 10.0% of carboxyvinyl polymer, 0.2% of triethanolamine, the amount of flavor, the amount of preservative, and the amount of purified water remaining.

&Lt; Preparation of comparative cosmetic composition 1. Production of soap >

A soap was prepared in the same manner as in Cosmetic Preparation Example 1 except that the composition of Example 1, Comparative Example 1 and Comparative Example 2 was used instead of the composition of Example 2 contained in the emulsion.

Soap condition Composition contained in soap Comparative cosmetic preparation Examples 1-1 Example 1-1 Comparative cosmetic preparation Examples 1-2 Examples 1-2 Comparative cosmetic preparation Examples 1-3 Example 1-3 Comparative cosmetic preparation Examples 1-4 Examples 1-4 Comparative cosmetic preparation Examples 1-5 Examples 1-5 Comparative cosmetic preparation Examples 1-6 Comparative Example 1-1 Comparative cosmetic preparation Examples 1-7 Comparative Example 1-2 Comparative cosmetic preparation Examples 1-8 Comparative Example 1-3 Comparative cosmetic preparation Examples 1-9 Comparative Example 1-4 Comparative cosmetic preparation Examples 1-10 Comparative Example 1-5 Comparative cosmetic preparation Examples 1-11 Comparative Example 1-6 Comparative cosmetic preparation Examples 1-12 Comparative Example 1-7 Comparative cosmetic preparation Examples 1-13 Comparative Example 1-8 Comparative cosmetic preparation Examples 1-14 Comparative Example 2-1 Comparative cosmetic preparation Examples 1-15 Comparative Example 2-2 Comparative cosmetic preparation Examples 1-16 Comparative Example 2-3 Comparative cosmetic preparation Examples 1-17 Comparative Example 2-4 Comparative cosmetic preparation Examples 1-18 Comparative Example 2-5 Comparative cosmetic preparation Examples 1-19 Comparative Example 2-6 Comparative cosmetic preparation Examples 1-20 Comparative Example 2-7 Comparative cosmetic preparation Examples 1-21 Comparative Example 2-8 Comparative cosmetic preparation Examples 1-22 Comparative Example 2-9 Comparative cosmetic preparation Examples 1-23 Comparative Example 2-10 Comparative cosmetic preparation Examples 1-24 Comparative Example 2-11 Comparative cosmetic preparation Examples 1-25 Comparative Examples 2-12 Comparative cosmetic preparation Examples 1-26 Comparative Example 2-13 Comparative cosmetic preparation Examples 1-27 water

&Lt; Comparative cosmetic preparation example 2. Preparation of cream >

A cream was prepared in the same manner as in Cosmetic Preparation Example 5 except that the composition of Comparative Example 2-1 was used instead of the composition of Example 2-1.

&Lt; Preparation example of pharmaceutical composition 1. Preparation of external preparation for skin >

The composition of Example 2-1 was mixed with white petrolatum so that the composition of Example 2-1 was 1.0% by weight to prepare an external composition for skin improvement for acne.

<Experimental Example 1> Confirmation of antimicrobial effect against acne bacteria>

In order to confirm the antibacterial effect of the compositions of Examples 1 and 2 and Comparative Examples 1 and 2, each composition was classified into Propionibacterium acnes causing acne, Staphylococcus aureus causing atopy ( Staphylococcus aureus ), respectively.

For this purpose, Propionibacterium acnes was cultivated in RCB (reinforced clostridial broth) at 37 ° C for 72 hours and sterilized in a liquid medium suspended in RCA (reinforced clostridial agar) plate medium to contain 1 × 10 ⁶ CFU / ml Staphylococcus aureus was uniformly plated on a cotton swab and incubated in a TSB (tryptic soybean broth) at 37 ° C for 24 hours, and uniformly suspended in a TSA (tryptic soybean agar) plate medium in which the liquid medium was uniformly sterilized . Next, each composition was diluted with DMSO (dimethyl sulfoxide) in the same volume, and the diluted solution (35 μl) was placed on a paper disk (diameter: 8 mm), and then placed on a plate medium on which Propionibacterium acnes and Staphylococcus aureus were streaked After incubation, the propionibacterium acnes was incubated at 37 ° C for 72 hours and the staphylococcus aureus was incubated at 37 ° C for 24 hours.

Thereafter, the effect of the diluted solution of each sample on Propionibacterium acnes and Staphylococcus aureus was investigated with a low supporting diameter, and it is shown in Table 7.

Composition condition Antimicrobial Effect of Propionibacterium acnes (mm) Antibacterial effect of Staphylococcus aureus (mm) Example 1-1 15 13 Examples 1-2 14 12 Example 1-3 13 13 Examples 1-4 14 13 Examples 1-5 15 12 Example 2-1 25 27 Example 2-2 27 29 Example 2-3 25 24 Examples 2-4 26 23 Example 2-5 26 27 Examples 2-6 24 23 Comparative Example 1-1 11 12 Comparative Example 1-2 11 11 Comparative Example 1-3 10 12 Comparative Example 1-4 10 10 Comparative Example 1-5 10 9 Comparative Example 1-6 10 10 Comparative Example 1-7 12 11 Comparative Example 1-8 10 11 Comparative Example 2-1 16 13 Comparative Example 2-2 15 14 Comparative Example 2-3 13 13 Comparative Example 2-4 10 9 Comparative Example 2-5 12 11 Comparative Example 2-6 13 12 Comparative Example 2-7 12 13 Comparative Example 2-8 13 12 Comparative Example 2-9 12 13 Comparative Example 2-10 12 13 Comparative Example 2-11 12 11 Comparative Examples 2-12 13 12 Comparative Example 2-13 12 11

Referring to Table 7, it can be confirmed that the antimicrobial effect of the composition of Example 2 is superior to that of other compositions.

<Experimental Example 2> Confirmation of atopy treatment effect>

Human monocyte THP-1 cells (human acute monocytic leukemia cells) were treated with antibiotics (penicillin 10 ⁴ U / ml streptomycin 10 mg / ml amphotericin B 25 μg / ml) and FBS (fetal bovine serum) The cells were cultured in RPMI 1640 medium containing 5% (v / v) at 37 ° C and 5% CO ₂ for 3 days. Thereafter, the THP-1 cells were divided into 24 well plates at a concentration of 2.0 x 10 &lt; ⁶ &gt; / ml using trypsin-EDTA to measure the activity of MCP-1, IL-6 and IL- And cultured in RPMI supplemented with 0.5% (v / v) at 37 ° C, 5% CO ₂ for 16 hours. Next, a tick extract (1 μg / ml) was treated with an atopy-inducing source in each well, and a sample (200 μg / ml) of Example 2 and Comparative Example 2 diluted with DMSO (dimethyl sulfoxide), dexamethasone , 50 / / ml) for 14 hours (DMSO was treated with the same volume of the solvent as the cell culture medium) for 14 hours (enzyme-linked immunospecific assay; Cytokine. 2008, 42 (3) ), The amounts of MCP-1, IL-6 and IL-8 in the supernatant were measured and shown in Table 8. At this time, the amount of each cytokine showed a fold value compared with 1.00 for cells treated with mite extract only.

Condition MCP-1 (fold) IL-6 (fold) IL-8 (fold) Control group 0.12 0.13 0.15 Mite extract 1.00 1.00 1.00 Example 2-1 0.25 0.38 0.24 Example 2-2 0.24 0.24 0.36 Example 2-3 0.33 0.25 0.15 Examples 2-4 0.26 0.33 0.24 Example 2-5 0.25 0.34 0.27 Examples 2-6 0.24 0.22 0.28 Comparative Example 2-1 0.83 0.87 0.76 Comparative Example 2-2 0.87 0.84 0.85 Comparative Example 2-3 0.93 0.85 0.84 Comparative Example 2-4 0.85 0.96 0.83 Comparative Example 2-5 0.82 0.98 0.92 Comparative Example 2-6 0.93 0.79 0.77 Comparative Example 2-7 0.91 0.83 0.83 Comparative Example 2-8 0.85 0.84 0.85 Comparative Example 2-9 0.87 0.85 0.74 Comparative Example 2-10 0.83 0.79 0.79 Comparative Example 2-11 0.79 0.88 0.94 Comparative Examples 2-12 0.88 0.85 0.78 Comparative Example 2-13 0.90 0.78 0.97 Dexamethasone 0.13 0.17 0.17

As shown in Table 8 above, treatment of mite extracts known to induce atopy in THP-1 cells results in increased secretion of MCP-1, IL-6 and IL-8. It was confirmed that the dexamethasone used as a positive control dropped the secretion of MCP-1 almost to the control (normal level). In the cells treated with the composition of Example 2 containing the herbal mixture extract of the present invention and the whitish oil, the MCP- 1, IL-6 and IL-8 secretion was significantly reduced, indicating that the composition has excellent therapeutic effect of atopic dermatitis.

<Experimental Example 3> Confirmation of skin moisturizing effect>

The cream of the cosmetic preparation example 5 of the present invention and the cream of the cosmetic preparation comparative preparation 2 were used to evaluate the skin moisturizing effect. Twenty women in their 20s and 40s in each group applied each cream sample to their right and left faces. They were then given a moisturizing power of 90 minutes before and after applying a 1cm downward part to the right side 3cm from the eye, using a Cornoemeter (CM810, Courage and Khazaka Electronic Co., Germany), and the results are shown in Table 9 below.

cream Composition in cream Corneometer (△ Hydration, g / m ² / h Cosmetic Preparation Example 5 Example 2-1 20.5 Comparative cosmetic preparation Example 2 Comparative Example 2-1 9.3

Referring to Table 9, it was confirmed that the skin moisturizing effect of the cosmetic preparation 5 cream containing the herbal medicine mixed extract of the present invention and the whitening oil of the present invention was superior to the cosmetic composition of Comparative Preparation Example 2 containing the comparative group sample .

<Experimental Example 4> Confirmation of acne improvement effect of soap>

The results are shown in Table 7 below. The results are shown in Table 7 below. The results are shown in Table 7 below. The results are shown in Table 7 below. 5 users).

The Korean Acne Grading System (KAGS), developed by a panel of experts in 2004, used face images of subjects who were photographed in a uniform size to represent acne lesions. The number of papules, nodules, ongoing scars, and sinus tracts were divided into six grades, grade 1 to grade 6, grade 1 to grade 11, grade 11 to grade 11 Grade 3, grade 3 if the papules are at least 30, grade 3 if the papules are at least 30, grade 3 at the nodule less than 10, 11-20 at the nodule, grade 4 at the progressive scarring and / or moderate progression at the nodule, If grade 5, nodules are more than 31 and / or moderate progression scars and / or grade 6. The KAGS grad was recorded at each visit and the results are shown in Table 10 below.

Soap condition Composition contained in soap Acne improvement (grade) Just before use After 3 days use After 1 week use After 4 weeks use Cosmetic Preparation Example 1-1 Example 2-1 3 2 2 One Cosmetic Preparation Example 1-2 Example 2-2 3 3 3 One Cosmetic Preparation Example 1-3 Example 2-3 3 3 3 One Cosmetic Preparation Examples 1-4 Examples 2-4 4 3 3 One Cosmetic Preparation Examples 1-5 Example 2-5 5 4 3 2 Cosmetic Preparation Example 1-6 Examples 2-6 5 4 3 One Comparative cosmetic preparation Examples 1-1 Example 1-1 4 4 4 3 Comparative cosmetic preparation Examples 1-2 Examples 1-2 4 4 4 3 Comparative cosmetic preparation Examples 1-3 Example 1-3 4 4 4 3 Comparative cosmetic preparation Examples 1-4 Examples 1-4 4 4 4 3 Comparative cosmetic preparation Examples 1-5 Examples 1-5 4 4 4 3 Comparative cosmetic preparation Examples 1-6 Comparative Example 1-1 5 5 5 5 Comparative cosmetic preparation Examples 1-7 Comparative Example 1-2 4 4 4 4 Comparative cosmetic preparation Examples 1-8 Comparative Example 1-3 4 4 4 3 Comparative cosmetic preparation Examples 1-9 Comparative Example 1-4 5 5 5 4 Comparative cosmetic preparation Examples 1-10 Comparative Example 1-5 4 4 4 4 Comparative cosmetic preparation Examples 1-11 Comparative Example 1-6 3 3 3 3 Comparative cosmetic preparation Examples 1-12 Comparative Example 1-7 4 4 4 4 Comparative cosmetic preparation Examples 1-13 Comparative Example 1-8 5 5 5 4 Comparative cosmetic preparation Examples 1-14 Comparative Example 2-1 4 4 4 4 Comparative cosmetic preparation Examples 1-15 Comparative Example 2-2 5 5 5 4 Comparative cosmetic preparation Examples 1-16 Comparative Example 2-3 6 6 6 5 Comparative cosmetic preparation Examples 1-17 Comparative Example 2-4 4 4 4 3 Comparative cosmetic preparation Examples 1-18 Comparative Example 2-5 5 5 5 4 Comparative cosmetic preparation Examples 1-19 Comparative Example 2-6 5 5 5 4 Comparative cosmetic preparation Examples 1-20 Comparative Example 2-7 4 4 4 4 Comparative cosmetic preparation Examples 1-21 Comparative Example 2-8 4 4 4 3 Comparative cosmetic preparation Examples 1-22 Comparative Example 2-9 4 4 4 3 Comparative cosmetic preparation Examples 1-23 Comparative Example 2-10 5 5 5 4 Comparative cosmetic preparation Examples 1-24 Comparative Example 2-11 4 4 4 4 Comparative cosmetic preparation Examples 1-25 Comparative Examples 2-12 4 4 4 3 Comparative cosmetic preparation Examples 1-26 Comparative Example 2-13 4 4 4 3 Comparative cosmetic preparation Examples 1-27 water 4 4 4 4

The results of Table 10 show that the cosmetic preparation Example 1 soap (the soap containing the composition of Example 2) is superior to the soap containing the composition of the other conditions in improving the acne, Is confirmed. In this case, a case in which the cosmetic preparation Example 1-1 soap (soap containing the composition of Example 2-1) was used to show the effect of treating acne was shown in FIG.

<Experimental Example 5> Confirmation of Skin Protection Effect of Soap>

Of the participants participating in the acne improvement test in Experimental Example 5, the soaps of the cosmetic preparation Example 1-1 and the cosmetic compositions of Comparative Examples 1-14 and 1-15 and 1-27 were used. The moisturizing effect is shown in Table 12 according to the criteria of Table 11 below.

Exfoliating / Moisturizing
Improvement effect Evaluation standard 5 Very good 4 good 3 usually 2 Poor One Very bad

Soap condition Composition contained in soap Exfoliation effect Skin moisturizing effect Cosmetic Preparation Example 1-1 Example 2-1 4.2 4.3 Comparative cosmetic preparation Examples 1-14 Comparative Example 2-1 3.2 3.5 Comparative cosmetic preparation Examples 1-15 Comparative Example 2-2 3.0 3.2 Comparative cosmetic preparation Examples 1-27 water 2.8 2.5

The results of Table 12 show that the soap prepared in the present invention has an excellent acne improvement effect, exfoliation effect and skin moisturizing effect.

<Experimental Example 6: Confirmation of atopy improvement effect of soap>

Adults 20 to 25 years old with atopic skin were asked to wash four times a day (morning and evening) twice daily for 5 groups in the same manner as in Experimental Example 4. The improvement of atopic appearance, the improvement of skin texture and the skin moisturizing effect according to the use of soap after 4 weeks were confirmed and shown in Table 14 according to the criteria of Table 13 below.

Atopy, keratin, moisturizing effect Evaluation standard 5 Very good 4 good 3 usually 2 Poor One Very bad

Soap condition Contained in soap
Composition atopy
Improvement effect corneous
Removal effect Skin moisturizing
effect Cosmetic Preparation Example 1-1 Example 2-1 4.3 4.5 4.1 Comparative cosmetic preparation Examples 1-14 Comparative Example 2-1 3.1 3.0 3.2 Comparative cosmetic preparation Examples 1-15 Comparative Example 2-2 3.3 3.2 2.9 Comparative cosmetic preparation Examples 1-27 water 2.7 2.7 2.3

The results of Table 14 show that the soap prepared in the present invention is also superior to atopic patients in atopic dermatitis, exfoliation and skin moisturizing effects.

Claims (10)

A composition comprising an extract of a herbal medicine mixture comprising a mixture of golden, yellow, white, and germanium and a monoaxic oil in a weight ratio of 10: 0.1 to 5,
The extract of the crude drug mixture may contain 30 to 40% (v / v) 1,3 (v / v) of the crude drug mixture comprising 50 to 200 parts by weight of Rhodiola, 50 to 200 parts by weight of yellowish white and 50 to 200 parts by weight of ginger -Butylene glycol aqueous solution,
The composition for preventing or treating acne or atopic dermatitis according to any one of claims 1 to 3, wherein the opacifying oil is an essential oil separated and extracted by steam distillation from an opacified wood. delete delete delete The method according to claim 1,
The composition containing the extract of the herbal medicine mixture containing the golden, yellow, white, and white gingiva and the whitening oil at a weight ratio of 10: 0.1 to 5 includes Propionibacterium acnes and Staphylococcus aureus Staphylococcus aureus ), which is effective for preventing or treating acne or atopic dermatitis. A cosmetic composition comprising an extract of a herbal medicine mixture comprising a mixture of golden, yellow, white, and germanium and a monoaxic oil at a weight ratio of 10: 0.1 to 5,
The extract of the crude drug mixture may contain 30 to 40% (v / v) 1,3 (v / v) of the crude drug mixture comprising 50 to 200 parts by weight of Rhodiola, 50 to 200 parts by weight of yellowish white and 50 to 200 parts by weight of ginger -Butylene glycol aqueous solution,
The cosmetic composition for improving acne or atopic dermatitis according to any one of claims 1 to 3, wherein the mannitol oil is an essential oil separated and extracted by steam distillation from a cottonwood. The method according to claim 6,
Wherein the cosmetic composition has an effect of moisturizing the skin or improving the appearance of the skin. delete delete A cosmetic for improving acne or atopic dermatitis, which comprises the cosmetic composition according to claim 6 or 7 as a raw material or an effective ingredient.

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