KR101064904B1 - Cosmetic composition for improving acne comprising natural extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving acne comprising rhubarb extract, eochocho extract, ginkgo extract and madecassoside as an active ingredient.
The composition according to the present invention is markedly enhanced at the same time antibacterial, skin regeneration, sebum secretion suppression, skin soothing and whitening function, there is almost no skin irritation has an excellent acne improvement and healing effect.

Description

Cosmetic composition for improving acne comprising natural extracts

The present invention relates to a cosmetic composition for improving acne, and more particularly, to a cosmetic composition having excellent acne-improving action, including a rhubarb extract, aeochocho extract, ginkgo extract and madecassoside as an active ingredient.

Acne is an inflammation of many hair follicles on the face of men and women of puberty, especially the cheeks and forehead. Some may develop on the chest or back, and some may develop at age 40. Initially, the hair follicles are full of cortex and keratin, resulting in yellowish lumps, sometimes with blackened peaks. When you squeeze the cheese will come out. It is then inflamed and reddish. When a bacterium is infected, it is concentrated and turns yellow. The better spot is a crater-shaped trail. Usually these four kinds are mixed. The cause is due to the infection of bacteria in the sebum excessively secreted by the action of congenital predisposition and sex hormones, especially male hormones. Women 22 years and older often worsen just before menstruation. The cause of acne is not yet clear, but it can generally be summarized as an increase in sebum secretion, keratinization and peroxidation of lipid components, proliferation of acne-causing bacteria, and inflammation and exacerbation. One of the important features related to the development of acne is the increase in sebum secretion, which is known to be closely related to the androgen, a male hormone. The secretion of male hormones above normal levels promotes sebaceous gland function, and the excess sebum is stagnated in the hair follicles due to the keratinization of the hair follicle wall, creating scleroderma. The sebum accumulated in the hair follicles, which cannot be discharged to the outside, blocks the pores and prevents the inflow of external air, so the anaerobic bacteria propionibacterium acnes can proliferate smoothly. Propionibacterium acnes secrete lipolytic enzymes and chemical factors to produce free fatty acids, trigger the immune response of leukocytes, and destroy the hair follicle wall, causing the contents to leak into the dermis, resulting in inflammatory reactions. . Therefore, the general treatment for acne can be summarized into four cases: suppression of excessive sebum production, inhibition of keratinization of the hair follicle wall, inhibition of proliferation of propionibacterium acnes, and minimization or inhibition of inflammatory response. .

Human skin contains a variety of microorganisms. These microorganisms cause various skin diseases. Staphylococcus aureus, which causes purulent skin, includes Staphylococcus epidermis and Propionibacterium acnes, which produce sebum and sweat from the skin. Decomposes to produce odors, and decompose products irritate and irritate the skin. In particular, acne bacteria (Propionibacterium acnes) breaks down the sebum using a fatty acid degrading enzyme (lipase) to produce free fatty acids. These free fatty acids not only irritate the skin but also cause inflammatory lesions such as papules, pustules and nodules, which are red rashes found in acne.

Although various medicines have been developed and used in the treatment area of acne, the selection of cosmetics for proper skin care has not been studied yet. In the cosmetics area, mainly soap, medicated soap, abrasive cleanser, etc., is a concept that heals acne by removing hair follicle narrowing through skin peeling. In addition, the alcohol component of astrigent also exfoliates the skin and disinfects the acne. There are also cosmetics containing resorcinol, sulfur, salicylic acid and benzoyl peroxide. However, these raw materials have a lot of side effects and limitations such as skin irritation due to the restriction of the use concentration.

In addition, antibiotics such as erythromycine for the removal of acne bacteria, anti-inflamation ingredients for inflammation treatment, and hormone preparations for sebum control are used, but these are mainly ingredients in the pharmaceutical domain.

Until now, the product has been developed focusing on only one of the various mechanisms of acne described above, so that the healing effect is insignificant and the recurrence frequently occurs. In addition, there is no preventive concept, and there is no product of healing concept in the cosmetic field.

The present invention has been made to solve the above problems, including antibacterial, skin regeneration, sebum secretion, skin soothing and whitening function is significantly enhanced at the same time, including the rhubarb extract, eoseongcho extract, ginkgo extract and madecassoside as an active ingredient An object of the present invention is to provide a cosmetic composition for acne improvement that can heal and prevent acne.

Another object of the present invention to provide a method for preparing the cosmetic composition for improving acne.

As one embodiment, the present invention relates to a cosmetic composition for improving acne comprising a rhubarb extract, aeochocho extract, ginkgo extract and madecassoside as an active ingredient.

In the present invention, the rhubarb extract, eoseongcho extract, ginkgo extract and madecassoside may be included in a weight ratio of 1 to 2: 0.5 to 1: 0.5 to 1: 0.5 to 2.5.

The rhubarb extract, eoseongcho extract and ginkgo extract can be obtained by fermenting each raw material and ultrasonic extraction. At this time, the fermentation process can be carried out by inoculating the microorganisms of Bacillus genus in the raw material of sulfur, eoseongcho and ginkgo.

The composition of the present invention is characterized by having antibacterial, skin regeneration, sebum secretion inhibition, skin soothing and whitening at the same time.

In the present invention, the rhubarb extract, Echochocho extract, ginkgo extract and madecassoside may be included in an amount of 0.01 to 20% by weight based on the total composition.

The cosmetic composition can have a formulation such as solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray.

In another aspect, the present invention comprises the steps of: (a) wet heat treatment the raw material of sulfur, eoseongcho, ginkgo at 80 ~ 100 ℃ for 30 to 60 minutes; (b) inoculating the heat-treated raw material inoculated with Bacillus sp. microorganisms and then fermenting by dry culture for 24 to 48 hours at 30 ~ 60 ℃, pH 5 ~ 8; (c) ultrasonically extracting the fermented raw material using an organic solvent; And (d) adding a madecassoside to the extract; relates to a method for producing a cosmetic composition for improving acne comprising a.

The manufacturing method of the present invention may further include the step of removing the low molecular weight material after the dialysis process using the membrane filter of the molecular weight 10kD after the ultrasonic extract. In addition, the ultrasonic extraction of step (c) may be carried out at a temperature of 3 ~ 50 ℃.

The cosmetic composition according to the present invention, including antibacterial, skin regeneration, sebum secretion, sebum secretion suppression, skin soothing and whitening function is significantly improved at the same time, including rhubarb extract, Echochocho extract, ginkgo extract and madecassoside, almost no skin irritation improve acne And an excellent healing effect.

Therefore, the cosmetic composition of the present invention can be usefully used in the development of cosmetics for various acne improvement, healing and prevention.

1 is an antimicrobial activity against propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermis of mixed fermentation extracts containing optimal concentrations of rhododendron, eochocho, ginkgo and madecassoside.
Figure 2 is a photograph taken showing the degree of acne improvement and healing of the test subjects after using the cosmetics of Comparative Example 4 and Example 2.

The cosmetic composition for improving acne of the present invention is characterized in that it comprises a rhubarb extract, aeochocho extract, ginkgo extract and madecassoside as an active ingredient.

Hereinafter, the configuration of the present invention in more detail.

As used herein, the term "acne" means a chronic, refractory, inflammatory skin disease that occurs in hair follicles and sebaceous glands.

Among the raw materials included in the composition of the present invention, golden thread (Golden thread, scientific name: Coptis chinensis) is a material that has been used in herbal medicine for a long time since it is used as a medicinal herb of the herbaceous perennial herbaceous plant. It is a plant. Nasturtium contains berberine as its main ingredient, as well as palmatine and alkaloids such as coptisine, warenine, and columbamaine, which prevents anti-inflammatory activity and atherosclerosis. The effect is known.

Houttuyniae herba (Huttuynia cordata) is a perennial herb of the Saururaceae family. Has long been used as a disinfectant, diuretic and antipyretic. In addition, the effects of diuretic, detoxification, cardiac, antipyretic, drainage, anticancer action is known.

Ginkgo (Ginkgo biloba Linnaeus) is a deciduous arborescent tree belonging to the Ginkgo family, and is distributed in the temperate zone except the alpine and highlands of China, Korea, and Japan, and consists of stems, leaves, and fruits. These banks are known to have blood circulation promoting and metabolic functions, including flavonoid glycoside components.

The Madecasoside component is a substance isolated from Centella asiatica. Centella asiatica is a small herbaceous plant belonging to the Umbelliferae family, which is tropical and coarse and wild at 600 to 800 meters above sea level, and is known to contain asiaticoside, madecassoside, asiatic acid and madecassic acid.

The extract of each plant raw material contained in the composition of the present invention may be used by extracting the raw material in a dry or natural state, in order to increase the extraction efficiency, may be used by segmentation or grinding. In addition, each plant extract may be extracted from various organs or parts, that is, fruit, leaves, flowers, stems, branches and bark. In the case of ginkgo it may be preferably extracted from the fruit.

When the composition of the present invention comprises a rhubarb extract, aeochocho extract, ginkgo extract and madecassoside at the same time, synergism occurs between the useful components of each material to effectively enhance the anti-aging, inflammation suppression, skin whitening or wrinkle improvement effect When included in a certain composition ratio, this effect can be further enhanced. The composition of the present invention may contain the rhubarb extract, Eochocho extract, ginkgo extract and madecassoside in a weight ratio of 0.1 to 2.0: 0.1 to 1.0: 0.1 to 1.0: 0.5 to 5.0, more preferably 1.0 to 2.0: 0.5 It may be contained in a weight ratio of ˜1.0: 0.5 to 1.0: 0.5 to 2.5, and most preferably, may be contained in a weight ratio of 2.0: 1.0: 0.5: 2.5. In this mixing ratio range, it can be expected excellent anti-acne improvement effect through antibacterial, skin regeneration, sebum secretion, skin soothing and whitening function without skin irritation.

In the present invention, sulfur extract, Echochocho extract, ginkgo extract and madecassoside may be included in an amount of 0.01 to 20% by weight, preferably 0.1 to 10% by weight, and more preferably 1 to 5% by weight, based on the total weight of the composition. . Within this content range, enhanced acne improvement can be expected without skin irritation.

The composition of the present invention may be used in addition to the well-known natural product extracts or other ingredients for improving acne in the range that does not inhibit the effective activity of the present invention in addition to rhubarb extract, Echochocho extract, ginkgo extract and madecassoside.

In addition, the composition may be molded into a specific formulation, including components conventionally added to cosmetic compositions, such as conventional auxiliaries or carriers such as stabilizers, solubilizers, vitamins, pigments and flavorings.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, powder, pact, lip gloss, lipstick, shadow, shampoo and conditioner It can be prepared in the formulation of.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

On the other hand, in the present invention, the sulfur extract, eochocho extract, ginkgo extract can be prepared using a conventional solvent, under the conditions of the usual temperature and pressure. As the extraction solvent, for example, water, lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, ether, chloroform and 1,3 butylene glycol may be used alone or in combination, preferably Is ethyl alcohol, most preferably 80% ethyl alcohol, but is not limited thereto. The extraction solvent may be extracted by mixing about 1 to 20 times based on the weight of the dried material. In addition, as the extraction method, heat extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction, and the like may be used, and may be used by repeatedly extracting one or several times.

The extraction temperature is not particularly limited as long as the extraction temperature is such that the effective activity of the useful component of each material is not removed. For example, it can be extracted by depositing at room temperature, and may be 50 ~ 100 ℃, and more preferably 60 to 80 ℃ when heated extraction using a common solvent. In addition, it is preferable to carry out low temperature extraction at 3-50 degreeC at the time of ultrasonic extraction, More preferably, it is 4-20 degreeC.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the extract of the present invention. Extracts of the present invention may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In particular, the rhubarb extract, eoseongcho extract and ginkgo extract in the present invention may be prepared by fermenting each raw material and ultrasonic extraction. Extracts prepared through this process exhibit more enhanced acne improvement than conventional heat extraction or deposition extraction.

At this time, the fermentation may proceed by inoculating Bacillus sp.

Specifically, the method for producing a cosmetic composition for improving acne of the present invention comprises the steps of: (a) wet heat treatment for 30 to 60 minutes at 80 to 100 ° C. in a raw material of sulfuric acid, eoseongcho; (b) inoculating the heat-treated raw material inoculated with Bacillus sp. microorganisms and then fermenting by dry culture for 24 to 48 hours at 30 ~ 60 ℃, pH 5 ~ 8; (c) ultrasonically extracting the fermented raw material using an organic solvent; And (d) adding madecassoside to the extract.

In the preparation method of the present invention, the yellow lotus, fish vinegar, ginkgo biloba extract and madecassoside may have a mixing ratio of 0.1 to 2.0: 0.1 to 1.0: 0.1 to 1.0: 0.5 to 5.0 based on the weight of the dry powder. For example, 1.0 ~ 2.0: 0.5 ~ 1.0: 0.5 ~ 1.0: 0.5 ~ 2.5.

In the extraction process of steps (a) to (c), the raw material of hull, eoseongcho and ginkgo can be mixed from the beginning to proceed with the extraction process, or after the extraction process separately for each raw material can be used by mixing the extract obtained have.

The wet heat treatment in step (a) may proceed for 30 to 60 minutes at a temperature of 80 ~ 100 ℃, preferably 45 ~ 60 minutes at 90 ~ 100 ℃. This process can reduce the microbial contamination of the raw materials.

Bacillus microorganisms are inoculated into the raw material heat-treated in the step (b) and the fermentation process is carried out, specifically, the fermentation conditions at 30 ~ 60 ℃ temperature, pH 5 ~ 8, anaerobic fermentation for 24 to 48 hours The condition may be carried out by dry culture. Preferably it can be carried out for 24 to 36 hours at 40 ~ 50 ℃, pH 6-7. Under such fermentation conditions, optimum raw material fermentation is possible, and the extraction efficiency of useful ingredients of each raw material is increased in the subsequent extraction process.

The organic solvent in step (c) may be used alone or mixed with a lower alcohol or purified water such as methanol, ethanol and butylene glycol, the organic solvent may be used in 1 to 20 times based on the mass of the raw material solids, Preferably it is 1-5 times. In addition, the ultrasonic extraction process can be carried out at a low temperature, preferably low temperature of 3 ~ 50 ℃, preferably 4 ~ 20 ℃. Through such a low temperature ultrasonic extraction process, it is possible to further increase the efficiency of extracting useful components of each raw material.

The natural product fermentation extract obtained through the ultrasonic extraction process is preferably concentrated and purified.

The manufacturing method of the present invention may further comprise the step of removing the low molecular weight material after the step (c), the ultrasonic extract through a dialysis process using a membrane filter having a molecular weight of 10kD.

The dialysis process is an additional purification process, and through this dialysis process, it is possible to remove low molecular weight substances present in the extract.

The natural product fermentation extract obtained through the manufacturing process can be used by lowering the viscosity of the extract by adding distilled water in the same volume as the separated solution, and freeze-drying, it is possible to determine the productivity by measuring the dry weight.

The characteristics of the genus Bacillus strains that can be used in the present invention are gram-positive bacteria, endogenous spore-forming ability, and has the form of enterococci. In addition, it is possible to grow under aerobic or anaerobic conditions, but not under anaerobic conditions, and has the characteristics of catalase production, casein, gelatin, and starch resolution. As a growth condition, it is possible to grow at a temperature of about 45 ° C. under 7% saline, and fermentation conditions, and growth is active at about pH 5.0 to 7.5. In addition, glucose, arabinose, xylose, and mannitol has the characteristic of generating an acid.

[ Example ]

Hereinafter, the configuration and operation of the present invention through the preferred embodiment of the present invention will be described in more detail. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited to the following examples. Details that are not described herein will be omitted since those skilled in the art can sufficiently infer technically.

Manufacturing example  1. Preparation of extract using immersion extraction

After grinding 100g each of dried yellow rhubarb, eoseongcho and ginkgo, it was immersed in 1.0l of 80% ethyl alcohol for 5 days at room temperature, filtered on 300 mesh and left for 1 week, and then the precipitate was washed with watman 5 filter paper. Filtered. Then, the resultant was concentrated to dryness at 80 ° C. using a vacuum vacuum concentrator to obtain each plant extract in powder form.

Test Example  One. P. acnes , S. aureus , S. epidermidis Antimicrobial activity against

<1-1> Antibacterial activity of each raw material extract

Propionibacterium acnes ( Propionibacterium) using each raw material extract prepared in Preparation Example 1 acnes), Staphylococcus aureus (Staphylococcus aureus), Staphylococcus epi der Miss (Staphylococcus epidermidis ) was measured for antimicrobial activity. The antibacterial activity was measured after incubating the disk with the sulfur extract, Echochocho, and Ginkgo biloba on solid agar medium coated with each test strain. Each mixed extract was mixed 1: 1 in the case of Hwangyeon + Eochocho, and 1: 1: 1 in the case of Hwangyeon + Eochocho + Ginkgo and then treated with the same weight of the extract. The antimicrobial activity was measured using the paper disc method, and the size of the transparent ring around the disc indicates the antimicrobial activity.

The results are shown in Table 1 and the size of the transparent ring around the disk by Propionibacterium Acnes, Staphylococcus aureus, Staphylococcus epidermis is shown in FIG.

Extract Type P.acne Inhibitory Activity S. aureus inhibitory activity S.epidermidis Inhibitory Activity Huang Yan (A) ++ ++ ++ Husband + Eoseongcho (B) +++ ++ ++ Husband + Eoseongcho + Bank (C) +++ +++ ++ -: Can't create transparent ring,-+: Strain growth after transparent ring creation, +: Weak transparent ring generation
++: create strong transparent ring, +++: create very strong transparent ring

As shown in Table 1 and Figure 1, it was confirmed that the antimicrobial activity is enhanced when the mixture of hump, eoseongcho and ginkgo, especially the antimicrobial activity against Propionibacterium Acnes and Staphylococcus aureus bacteria is further increased It was confirmed.

<1-2> Antibacterial activity according to the blending ratio of each raw material extract

In the Preparation Example 1, the yellow antibacterial, fish starch, ginkgo extract and madecassoside were mixed in the component ratios (based on the dry powder) of Table 2 to prepare a natural antimicrobial formulation. About the compounding ratio of each component, the antimicrobial activity was measured by the same antimicrobial activity measuring method as Test Example 1.

Huang Yan: Eoseongcho: Bank: Madecasoside blending ratio P. acne inhibitory ability S.aureus Inhibitory S.epidermidis Inhibitory Activity 1.0: 1.0: 1.0: 1.0 +++ +++ ++ 2.0: 1.0: 0.5: 2.5 +++ +++ +++ 1.0: 2.0: 2.0: 1.0 ++ ++ ++ 1.0: 2.0: 3.0: 2.0 ++ ++ ++ 1.5: 1.0: 0.5: 0.5 ++ ++ +

As a result, it was confirmed that rhubarb, eoseongcho, ginkgo biloba extract and madecassoside showed the best antibacterial effect when formulated in a ratio of 2: 1: 0.5: 2.5.

<1-3> Antimicrobial activity of each raw material extract concentration

The antimicrobial agent was formulated at various concentrations (0.5%, 1.0%, 2.0%, 5.0) in a combination ratio (half salt, fish vinegar, ginkgo extract and madecassoside to be 2: 1: 0.5: 2.5) showing the highest antimicrobial activity in the experiment. %), The antimicrobial activity was measured according to the concentration, and the antimicrobial activity was measured by the same antimicrobial activity measurement method as in Test Example 1.

Microbial strain P. acne S. aureus S. epidermidis Concentration (a) 0.5 1.0 2.0 5.0 0.5 1.0 2.0 5.0 0.5 1.0 2.0 5.0 Suppression degree (b) + + ++ +++ -+ + ++ ++ -+ + ++ +++ a: Concentration (%) of mixed natural antimicrobial agent per disc
b:-: No transparent ring formation,-+: Strain growth after transparent ring generation, +: Weak transparent ring generation
++: create strong transparent ring, +++: create very strong transparent ring

As a result, as the concentration was increased, the antimicrobial activity was confirmed to increase.

Example  1. For acne improvement through conventional extraction methods Cosmetics  Composition manufacturing

The rhubarb, eoseongcho, ginkgo extract and madecassoside obtained in Preparation Example 1 were mixed to 2: 1: 0.5: 2.5 to prepare a cosmetic composition for improving acne.

Example  2-6, Comparative example  1 to 3. For improving acne through fermentation and ultrasonic extraction process Cosmetics  Produce

Each of the raw materials of sulfur, fish, and ginkgo were subjected to wet heat treatment at 80 to 100 ° C. for 30 minutes to reduce microbial contamination. Bacillus subtilis ( Bacillus subtilis) subtilis ) strains were inoculated and dried for 24 hours at 40 ℃, pH 6.5 for anaerobic fermentation conditions to ferment each raw material.

The fermented raw material was ethanol in a solid weight ratio of 1: 1 (at the same weight as the solid mass) to extract ultrasonically at a temperature of about 40 ° C. at low temperature, to recover the extract, and to concentrate and purify the natural fermentation extract in a dry powder state. Got it.

Madecasoside was added to the natural fermented extract to prepare a cosmetic composition for improving acne.

The rhubarb, eoseongcho, ginkgo extract and madecassoside were mixed to the weight ratio of the following Table 4 on the basis of the dry powder of the extract.

Kinds Huang Yan: Eoseongcho: Bank: Madecassoside Mixing Ratio Example 2 2.0: 1.0: 0.5: 2.5 Example 3 1.0: 1.0: 1.0: 1.0 Example 4 1.0: 2.0: 2.0: 1.0 Example 5 1.0: 3.0: 2.0: 2.5 Example 6 2.0: 0.5: 3.0: 1.0 Comparative Example 1 1: 1: 0: 1 Comparative Example 2 1: 0: 1: 1 Comparative Example 3 0: 1: 1: 1

Using the cosmetic composition prepared in the compositions of Examples 1 to 6 and Comparative Examples 1 to 3, each cream was prepared for improving acne using a conventional cream manufacturing method. Formulation composition in the preparation of the cream was set forth in Table 5.

ingredient weight% Mixed composition (each composition of Examples 1-6 and Comparative Examples 1-3) 5.00 Purified water to 100 glycerin 6.00 1,3-butylene glycol 4.00 Carbomer 0.20 Hyaluronic acid 1.00 Allantoin 0.10 Disodium ID 0.05 Betaine 1.00 Cetearyl Alcohol 1.50 Glyceryl Stearate 1.00 Beads wax 1.00 Fiji-100 Stearate 1.50 Cetylethylhexanoate 5.00 Cyclomethicone 7.00 Sunflower Seed Oil 6.00 Tocopheryl acetate 0.05 Squalane 4.00 Triethanolamine 0.15 antiseptic a very small amount Spices a very small amount

Comparative example  4

Except for the mixed composition in Table 4, the cream was prepared using the remaining ingredients.

Comparative example  5

A commercial acne cream was prepared.

Test Example  2. Skin irritation test

Skin patch test was carried out to investigate the skin irritation test for each of the cosmetics prepared with the compositions of Examples 1 to 6 and Comparative Examples 1 to 3. The subjects were selected as those who did not have skin disease at the test site of 15 ~ 30 years old. After wiping the upper part of the patch with 70% ethanol and drying, 0.1g of the product was added to the upper arm for 24 hours, the skin reaction was examined within 1 hour after the patch was removed, and the next day (after 48 hours) was examined again. . The skin reaction was judged by the following test criteria. The skin patch test results are shown in Table 6.

* Skin test criteria

- no response

± weak positive reaction (erythema)

+ Positive (erythema)

++ strong positive reaction (erythema, edema)

+++ Severe positive reactions (erythema, edema, blisters)

Prescription Number of examinees (person)
Judgment result - ± + ++ +++ Example 1 15 15 0 0 0 0 Example 2 15 15 0 0 0 0 Example 3 15 15 0 0 0 0 Example 4 15 14 One 0 0 0 Example 5 15 13 2 0 0 0 Example 6 15 14 One 0 0 0 Comparative Example 1 15 13 2 0 0 0 Comparative Example 2 15 13 2 0 0 0 Comparative Example 3 15 13 2 0 0 0

As shown in Table 6, the cosmetic prepared by the production method of the present invention was shown to have little skin irritation.

Test Example  3. Evaluation of Acne Improvement

In the case of using the cosmetics of Example 2 and Comparative Example 4 evaluated the degree of acne improvement and healing. The subjects of 20 subjects were treated with the cosmetics of Comparative Example 4 on the left side and the cosmetics of Example 2 on the right side after daily cosmetics, and then the degree of acne improvement and healing was evaluated after 2 weeks. The results are shown in Table 7 and FIG. 2.

division Acne improvement and healing persons % Example 2 very good 8 40 satisfied 5 25 usually 4 20 Slightly dissatisfied 2 10 dissatisfaction One 5 Comparative Example 4 very good 0 0 satisfied One 5 usually 6 30 Slightly dissatisfied 6 30 dissatisfaction 7 35

As a result, as shown in Table 7 and Figure 2, when using the cosmetic composition of Example 2 containing the composition according to the present invention, it was confirmed that the acne improvement effect is excellent.

Test Example  4. Evaluation of skin improvement effect

The cosmetics of Examples 1 to 6 and Comparative Examples 1 to 3 and 5 were evaluated for skin regeneration, sebum secretion inhibition, whitening effect, skin calming effect and overall preference including acne improving effect (antibacterial effect). Specifically, the cosmetics of Examples 1 to 6 and Comparative Examples 1 to 3 and 5 were used for 300 people for one month (at least once a day), and then the 10-point scale method (1: very bad) was used. -> 10: very good) and then tested for significance with Duncan's multiple range test. As a result, the results shown in Table 8 were obtained.

Evaluation items Example
One Example
2 Example
3 Example
4 Example
5 Example
6 Comparative example
One Comparative example
2 Comparative example
3 Comparative example
5 Acne improvement
(Antibacterial effect) 8.8 ± 0.22 9.5 ± 0.43 9.1 ± 0.356 9.0 ± 0.94 8.5 ± 0.91 7.6 ± 0.24 7.1 ± 0.51 6.1 ± 0.17 7.0 ± 0.83 6.9 ± 0.11 Skin regeneration 8.1 ± 0.58 8.8 ± 0.23 8.3 ± 0.84 7.7 ± 0.17 8.0 ± 0.12 7.6 ± 0.87 7.2 ± 0.55 7.0 ± 0.24 6.9 ± 0.56 5.1 ± 0.82 Sebum secretion suppression 8.2 ± 0.97 9.0 ± 0.87 8.5 ± 0.13 8.1 ± 0.83 7.9 ± 0.64 7.5 ± 0.42 7.0 ± 0.66 6.7 ± 0.54 6.8 ± 0.29 6.2 ± 0.93 Whitening effect 7.1 ± 0.911 7.6 ± 0.16 7.5 ± 0.55 6.7 ± 0.83 6.5 ± 0.61 6.2 ± 0.88 5.4 ± 0.95 5.3 ± 0.73 5.1 ± 0.13 3.5 ± 0.64 Skin soothing effect 8.1 ± 0.24 8.9 ± 0.58 8.3 ± 0.21 8.2 ± 0.73 8.0 ± 0.83 7.9 ± 0.29 7.4 ± 0.13 7.0 ± 0.16 6.7 ± 0.25 6.8 ± 0.67 Overall preference 8.4 ± 0.11 9.2 ± 0.86 8.9 ± 0.97 8.6 ± 0.59 8.5 ± 0.43 8.3 ± 0.66 7.2 ± 0.81 7.0 ± 0.67 6.8 ± 0.99 6.2 ± 0.34

As a result, as shown in Table 8, the cosmetics prepared by the manufacturing method of the present invention is generally acne improvement effect (antibacterial effect), skin regeneration effect, sebum secretion inhibitory effect, whitening compared to commercially available acne improvement cosmetics The effect, soothing effect and overall preference were found to be good.

In particular, the cosmetic compositions of Examples 1 to 6 having the composition range of the present invention were superior in the improvement effect in all the items compared to the cosmetic compositions of Comparative Examples 1 to 3 out of the composition range, it was confirmed that the overall preference is high.

Through the skin improvement evaluation, the cosmetic according to the present invention was confirmed that not only the antibacterial effect, but also excellent skin regeneration, sebum secretion, skin soothing and whitening function, acne healing and improvement through comprehensive skin improvement.

Claims (10)

It contains the rye extract, Echochocho extract, ginkgo extract and madecassoside as active ingredients,
The rhubarb extract, eoseongcho extract and ginkgo extract is characterized in that obtained by fermenting each raw material and ultrasonic extraction, cosmetic composition for improving acne.
The method of claim 1,
The rhubarb extract, Eochocho extract, ginkgo extract and madecassoside are characterized in that the weight ratio of 1 to 2: 0.5 to 1: 0.5 to 1: 0.5 to 2.5, the cosmetic composition for improving acne.
delete The method of claim 1,
The fermentation is characterized in that to proceed by inoculating Bacillus sp.
The method of claim 1,
The composition is characterized in that it has antibacterial, skin regeneration, sebum secretion suppression, skin soothing and whitening function, cosmetic composition for acne improvement.
The method of claim 1,
The rhubarb extract, eoseongcho extract, ginkgo extract and madecassoside is characterized in that it comprises 0.01 to 20% by weight based on the total composition, cosmetic composition for improving acne.
The method of claim 1,
The cosmetic composition comprises a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Characterized in that having cosmetic composition for improving acne.
(a) wet heat-treating the rhubarb, eoseongcho, ginkgo raw materials at 80-100 ° C. for 30-60 minutes;
(b) inoculating the heat-treated raw material inoculated with Bacillus sp. microorganisms and then fermenting by dry culture for 24 to 48 hours at 30 ~ 60 ℃, pH 5 ~ 8;
(c) ultrasonically extracting the fermented raw material using an organic solvent; And
(d) adding madecassoside to the extract;
Method for producing a cosmetic composition for improving acne comprising a.
The method of claim 8,
And removing the low molecular weight material through the dialysis process using the membrane filter having a molecular weight of 10 kD after the step (c); further comprising the step of producing a cosmetic composition for improving acne.
The method of claim 8,
Ultrasonic extraction of the step (c) is characterized in that it is carried out at a temperature of 3 ~ 50 ℃, method for producing a cosmetic composition for improving acne.

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