KR20150067015A - Cosmetic composition for improving anti-oxidation, whitening and anti-allergic containing the extract of Broom - Google Patents

Cosmetic composition for improving anti-oxidation, whitening and anti-allergic containing the extract of Broom Download PDF

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KR20150067015A
KR20150067015A KR1020140140738A KR20140140738A KR20150067015A KR 20150067015 A KR20150067015 A KR 20150067015A KR 1020140140738 A KR1020140140738 A KR 1020140140738A KR 20140140738 A KR20140140738 A KR 20140140738A KR 20150067015 A KR20150067015 A KR 20150067015A
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extract
cosmetic composition
broom
whitening
present
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장문식
정욱순
엄선영
김영훈
박송아
이하연
문미연
임준환
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주식회사 내추럴솔루션
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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    • A61K8/9789—Magnoliopsida [dicotyledons]
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A61K8/064—Water-in-oil emulsions, e.g. Water-in-silicone emulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin

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Abstract

The present invention relates to a plant extract having activity of anti-oxidation, skin whitening, and allergy alleviation and to a cosmetic composition containing the same extract as an active ingredient. A method for producing the cosmetic composition includes the steps of: producing a broom extract; and identifying anti-oxidation activity, melanin synthesis inhibition activity, and activity of inhibiting cell degranulation of a mast cell with respect to the extract. The broom extract is obtained by an ultrasonic wave extraction method in addition to the method of the present invention and thus has excellent effects of whitening skin and alleviating allergy.

Description

브룸 추출물을 유효성분으로 함유하는 항산화, 미백 및 알러지 개선용 화장료 조성물{Cosmetic composition for improving anti-oxidation, whitening and anti-allergic containing the extract of Broom}TECHNICAL FIELD The present invention relates to a cosmetic composition for improving antioxidant, whitening and allergy containing an extract of Broome as an active ingredient,

본 발명은 브룸(Broom) 추출물을 유효성분으로 함유하는 항산화, 미백 및 알러지 개선용 화장료 조성물에 관한 것이다.
The present invention relates to a cosmetic composition for antioxidant, whitening and allergy improvement containing an extract of Broom (Broom) as an active ingredient.

식물에 있어 꽃은 종족 보존의 기능을 하기 때문에 화려한 색깔을 가지고 있으며 각각의 색깔별로 안토시아닌계, 카로티노이드계 및 플라보노이드계의 다양한 색소 성분들이 함유되어 다양한 기능을 나타내고 있다. 특히, 꽃에 색소에는 노랑, 오렌지, 분홍색 계열의 불용성 색소인 카로틴류와 크산토필류로 나뉘며 전자에는 β-carotene, Lycopene이 후자에는 Lutein, Zeaxanthin, Violaxanthin이 있다. 이러한 카로티노이드 색소는 항산화 작용, 면역증강 작용, 항암 작용, 혈관질환개선 작용, 노화억제 작용 등의 다양한 기능을 가지고 있다.Flowers in plants have a colorful color because they function as species conservation. Various colors of anthocyanins, carotenoids and flavonoids are contained in various colors. In particular, the pigment in the flower is divided into carotene and xanthophylls, which are yellow, orange, and pink insoluble pigments, and β-carotene, Lycopene in the former, and Lutein, Zeaxanthin and Violaxanthin in the latter. These carotenoid pigments have various functions such as antioxidative action, immunity enhancing action, anticancer action, vascular disease improving action, and aging inhibiting action.

브룸(Broom)은 콩과(Leguminosae)의 금작화속(Cytisus)에 속하며 전 세계적으로 30종 이상 생육하고 있으며 노란꽃이 보통이지만 흰색, 홍황색, 담황색 꽃이 피는 것도 있다(Kim, S. I., Cytisus, 2008, Landscaring tree association, 107, 12-14). 브룸은 연구가 많이 되지 않은 꽃으로 조경으로 사용될 뿐 그 이용범위는 극히 제한되어 있는 실정이다.Broom belongs to the genus Cytisus of Leguminosae and grows more than 30 species globally, and yellow flowers are common but there are also white, reddish yellow and light yellow flowers (Kim, SI, Cytisus, 2008, Landscaring tree association, 107, 12-14). Broome is a flower that has not been studied much, and it is used as a landscape, but the range of its use is extremely limited.

피부는 과도한 자외선이나 오염물질에 노출되면 부종, 가려움 등의 피부자극 및 염증반응이 유발될 뿐만 아니라 염증 반응과정에서 생성되는 물질들이 부수적으로 색소침착과 피부 탄련섬유의 붕괴를 촉진시켜 피부 주름을 증가시킨다. 이러한 피부 트러블은 활성 산소와도 관련이 있음을 수많은 연구에 의해 알려져 있다. 활성 산소는 진피의 결합조직인 콜라겐, 엘라스틴, 히아루론산등을 파괴하여 피부의 주름을 일으키며 세포막의 지질 부분을 산화시켜 세포의 파괴 현상을 일으켜 피부염, 여드름, 피부암 등의 질병을 유발한다. 또한 이 활성 산소는 멜라닌 형성 과정 중 자발적인 산화반응에 관여하여 기미, 주근깨 등의 원인 및 주름생성의 원인이 되기도 한다(대한 화장품학회지 23권, 1호, 75-132).Exposure to excessive ultraviolet rays or pollutants causes skin irritation and irritation such as itching, as well as substances generated during the inflammatory reaction, which cause secondary collapse of pigmentation and collapse of skin-traction fibers to increase skin wrinkles . Many studies have shown that these skin troubles are also associated with active oxygen. Active oxygen destroys collagen, elastin, and hyaluronic acid, which are connective tissues of the dermis, causing wrinkles of the skin, oxidizing the lipid part of the cell membrane, causing cell destruction, and causing diseases such as dermatitis, acne and skin cancer. In addition, this active oxygen participates in the spontaneous oxidation reaction during the melanin formation process, and causes the causes such as stain and freckles and wrinkle formation (Korean Journal of Cosmetic Science, Vol. 23, No. 1, 75-132).

꽃 혼합 추출물을 함유하는 피부 미백용 화장료 조성물로서, 참취꽃, 유채꽃, 홍화, 금작화(Broom) 등의 꽃 혼합 추출물을 함유하는 화장료 조성물이 대한민국 등록특허 제10-1081059호에 개시된 바 있고, 아토피 피부 개선용 화장료 조성물로 베리류의 추출물을 함유하는 화장료 조성물이 대한민국 등록특허 제10-1287021호에 개시되어 있다. 또 섬오가피 추출물을 유효성분으로 함유하는 항산화, 항노화, 항염, 미백, 항주름 또는 항알러지용 화장료 조성물이 본 발명자들의 등록특허 제10-1238706호가 개시되어 있다. 그러나 상기문헌 어디에도 항산화, 미백 및 알러지 개선 효과가 있는 브룸 추출물에 관하여는 개시된 바 없다.The present invention relates to a cosmetic composition for skin whitening comprising a flower blend extract, a cosmetic composition containing a mixed blend of flowers such as a wild flower, rape flower, safflower, and broom, as disclosed in Korean Patent No. 10-1081059, A cosmetic composition containing an extract of berries as a cosmetic composition for improving skin is disclosed in Korean Patent Registration No. 10-1287021. In addition, Patent No. 10-1238706 of the present inventors discloses a cosmetic composition for antioxidant, anti-aging, anti-inflammation, whitening, anti-wrinkle or antiallergic containing an extract of Islet extract as an active ingredient. However, nothing has been disclosed in the above-mentioned literatures concerning the bloom extract having antioxidative, whitening and allergy improving effects.

따라서 본 발명의 목적은 항산화, 미백 및 알러지 개선 활성이 있는 브룸(Broom)의 추출물을 제공하는 데 있다.Accordingly, an object of the present invention is to provide an extract of Broom having antioxidative, whitening and allergic activity.

본 발명의 다른 목적은 상기 브룸(Broom)의 추출물을 함유하는 항산화, 미백 및 알러지 개선용 화장료 조성물을 제공하는 데 있다.
Another object of the present invention is to provide a cosmetic composition for antioxidant, whitening and allergy improvement containing the extract of Broom.

본 발명의 상기 목적은 본 발명 브룸 추출물을 제조하는 단계와; 상기 추출물의 항산화활성, 멜라닌 합성 저해능, 비만세포의 탈과립 억제활성을 확인하고 화장료 조성물로서 효능을 평가함으로써 달성하였다.
The above object of the present invention can be achieved by a method for producing a brome extract, comprising the steps of: preparing a brome extract of the present invention; The antioxidative activity, melanin synthesis inhibitory activity, and degranulation inhibitory activity of the extract of the extract were confirmed and evaluated by evaluating the efficacy as a cosmetic composition.

본 발명은 브룸 추출물의 제조시 물, 유기용매 혼합물에 초음파 추출법을 병행함으로써 항산화활성 및 미백활성이 증대될 뿐만 아니라 알러지 개선에 뛰어난 효과가 있다.
In the present invention, the combination of water and an organic solvent mixture in the preparation of a broom extract results in an increase in antioxidative activity and whitening activity as well as an excellent effect in allergy improvement.

도 1은 본 발명 공시재료로서 사용한 브룸의 사진도이다.
도 2는 본 발명 브룸 추출물의 항산화 효과를 나타낸 그래프이다.
도 3은 본 발명 브룸 추출물의 멜라닌 합성 저해능효과를 나타낸 그래프이다.
도 4는 본 발명 브룸 추출물의 항알러지 효과를 나타낸 그래프이다.1 is a photograph of Broome used as a disclosure material of the present invention.
2 is a graph showing the antioxidative effect of the broom extract of the present invention.
3 is a graph showing the effect of inhibiting melanin synthesis of the broom extract of the present invention.
4 is a graph showing the anti-allergy effect of the broom extract of the present invention.

본 발명은 브룸 추출물을 유효성분으로 함유하는 화장료 조성물을 제공한다.The present invention provides a cosmetic composition comprising a bloom extract as an active ingredient.

본 발명에 있어서 가장 바람직하기는 상기 브룸 추출물은 건조된 시료에 유기용매를 가하고 sonificator 온도를 40℃로 고정한 후 초음파 처리하여 제조한다. 그러나, 본 발명의 추출물은 물, 유기용매 또는 이들의 혼합용매 추출만으로도 가능하다. 이때 사용하는 유기용매는 에탄올, 부틸렌글리콜, 프로필렌글리콜, 프로판디올 등이다. 또, 상기 추출물에 의해 수득되는 추출액, 추출액의 희석액 또는 농축액, 추출액을 건조하여 수득되는 건조물 또는 추출액의 조정제물 또는 정제물일 수 있다.
In the present invention, most preferably, the broom extract is prepared by adding an organic solvent to a dried sample, fixing the sonificator at a temperature of 40 ° C, and then subjecting the extract to ultrasonic treatment. However, the extract of the present invention can be obtained only by water, an organic solvent, or a mixed solvent thereof. The organic solvent used herein is ethanol, butylene glycol, propylene glycol, propanediol, and the like. It may also be a dried product obtained by drying the extract obtained by the above extract, a diluted solution of the extract, a concentrate or an extract, or a preparation or a purified product of the extract.

본 발명에 있어서 상기 제조된 브룸 추출물은 화장료 조성물 총 중량의 0.1 ~ 10중량%로 바람직하게는 2중량%를 함유하는 것을 특징으로 한다. 0.1중량% 이하에서는 본 발명의 활성 산소종 저해효과가 미흡하게 나타나며, 10중량% 이상 사용하는 경우 최종 제품에서 고유 풍미에 따른 고유취와 색상이 강하게 나타나 제품 적용에 어려움이 있다.The broth extract according to the present invention is characterized by containing 0.1 to 10% by weight, preferably 2% by weight, of the total weight of the cosmetic composition. When the content is less than 0.1% by weight, the effect of inhibiting the active oxygen species of the present invention is insufficient. When the content is more than 10% by weight, the final product has inherent odor and color depending on its unique flavor.

본 발명에 따른 피부 보호 효과를 가지는 화장료 조성물은 통상의 제형을 가질 수 있으며, 예를 들어 수용성 리퀴드, 로션, 크림, 젤, 팩, 에센스, 수중유(O/W), 유중수(W/O)형 또는 실리콘중수(W/Si)형의 파우더로 제형화될 수 있다. 이들 각 제형의 조성물은 그 제형의 제제화에 필요하고 적절한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다.The cosmetic composition having a skin protecting effect according to the present invention may have conventional formulations and may be in the form of, for example, water-soluble liquid, lotion, cream, gel, pack, essence, oil / water, ) Or a silicone heavy (W / Si) type powder. The composition of each of these formulations may contain various kinds of bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

또한 본 발명에 따른 항산화 효과, 미백 및 알러지 개선 효과를 가지는 브룸 추출물을 함유한 화장료 조성물은 통상적인 사용방법에 따라 사용될 수 있으며, 사용자의 피부상태 또는 취향에 따라 그 사용횟수를 달리할 수 있다.
Further, the cosmetic composition containing the broom extract having the antioxidative effect, whitening effect and allergy improving effect according to the present invention can be used according to the usual use method, and the use frequency can be changed according to the skin condition or taste of the user.

이하, 본 발명을 실시예에 의해 보다 상세하게 설명한다. 그러나 하기 실시예는 본 발명을 예시하기 위한 것에 불과하며, 본 발명의 범위를 한정하는 것으로 의도되지 않는다.
Hereinafter, the present invention will be described in more detail by way of examples. However, the following examples are intended to illustrate the invention and are not intended to limit the scope of the invention.

<< 실시예Example > 본 발명 > Invention 브룸Broome 추출물의 제조 Preparation of extract

건조된 브룸(Broom) 2 ~ 3 g에 40% 프로판디올(propandiol) 40 ~ 60 mL를 첨가한 후 solificator(JAC 4020, Jinwoo, Korea)를 이용하여 온도를 40℃로 고정한 후 추출하여 본 발명 브룸 추출물을 얻었다. 모든 과정은 1시간씩 3회 반복하여 실시하였으며 상기 추출한 시료는 0.45 μm 막 필터로 여과한 후 효능시험을 진행하였다.
40 to 60 mL of 40% propanediol was added to 2 to 3 g of dried broom and the temperature was fixed at 40 ° C. using a solificator (JAC 4020, Jinwoo, Korea) An extract was obtained. All the procedures were repeated three times for 1 hour. The extracted samples were filtered with a 0.45 μm membrane filter, and then subjected to an efficacy test.

<< 실험예Experimental Example 1> 본 발명  1> invention 브룸Broome 추출물의 항산화 활성 실험 Antioxidant activity test of extract

본 실험예에서는 피부 섬유아세포(HDFn)를 DMEM(10% 혈청 첨가, 1% 항생제 포함) 배지에 현탁하여 37℃, 5% CO2 배양기에서 배양한 후 대수기에서 성장하는 세포를 실험에 사용하였다.In this experimental example, the skin fibroblast (HDFn) was suspended in DMEM (containing 10% serum and 1% antibiotic), cultured in a 5% CO 2 incubator at 37 ° C, .

Well 당 약 1×105 세포 수가 되도록 96 well에 각각 접종한 후 시료 추출물 0.5%, 1% 및 2%로 처리한 다음 37℃, 5% CO2 배양기에서 20분 동안 배양하였다. 산화스트레스 유발물질인 H2O2 1 mM을 첨가하고 DCFH-DA (stock 20 mM) 20 μM를 가한 후 spectrofluorophotometer(excitation 485 nm, emission 535 nm)로 측정하여 37℃에서 90분 동안 반응을 관찰하였다. 시료를 넣지 않고 활성 산소종 (Reactive oxygen species)의 형성만 측정한 것을 대조군으로 정하고, 시료를 넣어 활성 산소종을 소거시키는 시료 처치군과 비교하여 활성 산소종 저해율을 구하였다. 모든 실험은 3번 반복하였다.
Wells were inoculated in 96 wells at a concentration of about 1 × 10 5 cells per well, treated with 0.5%, 1%, and 2% of sample extracts, and then cultured in a 5% CO 2 incubator at 37 ° C for 20 minutes. 1 mM H 2 O 2 was added and 20 μM DCFH-DA (stock 20 mM) was added. The reaction was monitored by a spectrofluorophotometer (excitation 485 nm, emission 535 nm) at 37 ° C. for 90 minutes . The inhibition rate of reactive oxygen species was determined by comparing the formation of reactive oxygen species with the absence of the sample as a control group, and compared with the group treated with a sample to remove active oxygen species. All experiments were repeated 3 times.

Figure pat00001
Figure pat00001

실험결과, 도 2에 나타난 바와 같이 본 발명 브룸 추출물의 항산화 활성은 시간에 따라 증가하였다. 상기 두 추출물을 각각 농도별(0.5%, 1%, 2%)로 처리시 농도가 높을수록 증가율이 높았으며 특히 브룸 추출물 2% 처리시 20%의 활성 산소종 형성을 저해하였다.
As shown in FIG. 2, the antioxidant activity of the extract of the present invention increased with time. When the extracts were treated with 0.5%, 1%, and 2% of the extracts, the growth rate of the extracts was increased as the concentration increased. Especially, treatment with 2% bloom extract inhibited 20% of the active oxygen species.

<< 실험예Experimental Example 2> 본 발명  2> invention 브룸Broome 추출물의 멜라닌 합성  Melanin synthesis of extract 저해능Low performance 실험 Experiment

murine melanoma(B-16 F1) 세포를 적량의 FBS(fetal bovine serum)가 함유된 DMEM 배지로 6 well plate에 well당 1×105개로 접종한 후 5% CO2, 37℃에서 세포가 well 바닥에 약 80% 이상 부착될 때까지 배양하였다. 상기 배양액의 배지를 제거하고 시료를 적당한 농도로 희석된 배지로 교체한 후 5% CO2, 37℃에서 일정시간 다시 배양하였다. 그리고 상기 배양액의 배지를 제거하고 남은 세포를 PBS(phosphated buffer saline)로 세척하고, 이것을 트립신으로 처리하여 세포를 회수하였다. 상기 회수된 세포는 hematocytometer를 이용하여 세포수를 측정한 후 5,000 ~ 10,000 rpm으로 10분간 원심분리 한 다음 상등액을 제거하여 pellet을 얻었다. 상기에서 얻은 세포 pellet는 60℃에서 건조한 후 10% DMSO가 함유된 1 M 수산화나트륨액 100 μL 또는 적량의 cell lysis buffer를 넣어 60℃ 항온조에서 세포내 멜라닌을 얻었다. 상기에서 얻은 멜라닌을 함유한 반응액을 microplate reader로 405 nm에서 흡광도를 측정하여 세포 수 당 멜라닌 양 또는 일정 단백질 당 멜라닌 양을 측정하였다.murine melanoma (B-16 F1) cells a suitable amount of FBS (fetal bovine serum) is inoculated on a 6 well plate in the DMEM medium 1 × 10 5 pieces per well and then 5% CO 2, the cells are well bottom at 37 ℃ Lt; RTI ID = 0.0 &gt; 80% &lt; / RTI &gt; After removal of the medium of said culture medium and replace it with the medium to dilute the sample to an appropriate concentration was cultured a certain time in a 5% CO 2, 37 ℃. Then, the culture medium was removed, and the remaining cells were washed with PBS (phosphatized buffer saline), and treated with trypsin to recover the cells. The recovered cells were counted using a hematocytometer, centrifuged at 5,000 to 10,000 rpm for 10 minutes, and the supernatant was removed to obtain a pellet. The cell pellet obtained above was dried at 60 ° C. and then 100 μL of 1 M sodium hydroxide solution containing 10% DMSO or an appropriate amount of cell lysis buffer was added to obtain intracellular melanin in a 60 ° C. thermostat. The amount of melanin per cell or the amount of melanin per cell was measured by measuring the absorbance of the reaction solution containing melanin obtained above at 405 nm with a microplate reader.

실험 결과 도 3에서 나타난 바와 같이 본 발명 브룸 추출물을 농도별(0.5%, 1%, 2%)로 처리시 농도가 높을수록 멜라닌 감소능이 증가 하였으며 특히 브룸 추출물 2% 처리시 26%의 멜라닌 합성을 저해하였다.
Experimental Results As shown in FIG. 3, when the extract of Broome according to the present invention was treated at various concentrations (0.5%, 1%, 2%), the melanin reduction activity was increased as the concentration was increased. Respectively.

<< 실험예Experimental Example 3> 본 발명  3> invention 브룸Broome 추출물의 비만세포의  Extract of mast cells 탈과립Degranulation 억제 실험 Inhibition experiment

RBL-2H3 cell을 10% FBS를 함유한 DMEM(Dulbeccos modified Eagles medium)에 현탁시킨 후 24 well plate에 각 well당 2 × 105cell이 들어가도록 처리하였다. 상기 처리액에 각 well당 100 ng/ml의 mouse monoclonal IgE로 감작시킨 후 5% CO2 incubator에서 24 시간동안 배양한 후 상기 배양액의 세포를 well당 Tyrode buffer 500 uL를 사용하여 세척하였다. 상기 세척액에 농도별로 혼합 추출물을 처리한 후 37℃, 5% CO2 incubator에서 15분간 배양하고 한 시간 동안 DNP-BSA 100 ng/ml을 처리하였다. 상기 처리액 상층액에 substrate (1mM p-nitrophenyl-N-acetyl-D-glucosaminide) 50 uL 처리 후 37℃, 5% CO2 incubator에서 한 시간 동안 배양한 후 ELISA reader를 사용하여 405nm에서 흡광도를 측정한다.RBL-2H3 cells were suspended in DMEM (Dulbeccos modified Eagles medium) containing 10% FBS and treated with 2 × 10 5 cells per well in a 24-well plate. The treated solution was sensitized with 100 ng / ml mouse monoclonal IgE per well and cultured in a 5% CO 2 incubator for 24 hours. Then, the cells of the culture were washed with 500 uL of Tyrode buffer per well. The washing solution was treated with DNP-BSA at a concentration of 5% CO 2 incubator for 15 minutes at 37 ° C and treated with DNP-BSA at a concentration of 100 ng / ml for 1 hour. The supernatant of the treated solution was treated with 50 μL of substrate (1 mM p-nitrophenyl-N-acetyl-D-glucosaminide) and incubated at 37 ° C in a 5% CO 2 incubator for one hour. The absorbance was measured at 405 nm using an ELISA reader do.

실험 결과 도 4에서 나타난 바와 같이 본 발명 브룸 추출물을 농도별(0.5%, 1%, 2%)로 처리시 농도가 높을수록 비만세포의 탈과립 억제능이 증가하였으며 특히 Broom 추출물 2% 처리시 24%의 비만세포의 탈과립을 저해하였다.
As shown in FIG. 4, when the broom extract of the present invention was treated at various concentrations (0.5%, 1%, 2%), the degranulation inhibitory ability of mast cells was increased as the concentration was increased. And inhibited degranulation of mast cells.

이상 설명한 바와 같이 본 발명은 브룸 (Cytisus scoparius (L.) Link.) 유래의 추출물을 제공하는 효과가 있을 뿐 아니라 상기 추출물을 유효성분으로 함유하는 항산화, 미백 또는 알러지 예방 및 개선용 화장료 조성물을 제공하는 뛰어난 효과가 있으므로 화장품 산업상 매우 유용한 발명인 것이다.The present invention as described above is Broom (Cytisus scoparius (L.) Link.) as well as an excellent effect of providing a cosmetic composition for antioxidative, whitening or allergy prevention and improvement containing the above extract as an active ingredient, so that it is very useful in the cosmetics industry It is an invention.

Claims (2)

브룸(Cytisus scoparius (L.) Link.)에 프로판디올을 첨가한 후 40℃에서 초음파 처리한 다음 여과하여 제조된 브룸 추출물을 화장료 조성물 총중량에 대해 0.1 내지 10 중량 % 함유하는 것이 특징인 미백 또는 알러지 개선 기능성 화장료 조성물
Characterized in that it contains 0.1 to 10% by weight of the extract of Broome prepared by adding propanediol to Broome ( Cytisus scoparius (L.) Link.) And then ultrasonically treating it at 40 캜 and filtering the whole. Improved functional cosmetic composition
제 1항의 화장료 조성물이 수용성 리퀴드, 로션, 크림, 젤, 팩, 에센스 및 수중유(O/W)형, 유중수(W/O)형 또는 실리콘중수(W/Si)형의 파우더로 구성된 군에서 선택되는 어느 하나의 제형을 특징으로 하는 화장료 조성물.The cosmetic composition according to claim 1, wherein the cosmetic composition is a water-soluble liquid, a lotion, a cream, a gel, a pack, an essence and a powder composed of O / W type, W / O type or W / &Lt; / RTI &gt; or a pharmaceutically acceptable salt thereof.
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