KR20150094832A - Cosmetic composition containing an extract of mocordica charantia fruit as an active compotnet and method for preparing the extract of mocordica charantia fruit therefor - Google Patents

Abstract

The present invention relates to a cosmetic composition for prevention of skin aging, antioxidation, skin wrinkle improvement or skin whitening comprising an extract of lily of the valley as an active ingredient, and a process for producing the extract of lily of the valley. The cosmetic composition according to the present invention can be used as an active ingredient of various functional cosmetic compositions because of its effect of inhibiting skin damage by ultraviolet rays, melanin formation inhibitory effect, DOPA oxidation inhibitory effect, wrinkle improving effect and antioxidative effect.

Description

FIELD OF THE INVENTION The present invention relates to a cosmetic composition comprising an extract of Leucocephalus as an active ingredient and a method for preparing the extract of Leucocephalum fruit for the same. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition,

The present invention relates to a cosmetic composition containing an extract of lily of the valley as an active ingredient, and a process for producing the extract of lily of the valley for the same. More specifically, the present invention relates to a cosmetic composition having an effect of inhibiting skin damage, melanin formation inhibiting effect, DOPA oxidation inhibition effect, wrinkle improving effect and antioxidative effect, And to a method for producing the fruity fruit extract

In human skin, various physical and chemical changes occur during the aging process. The cause of this phenomenon is divided into intrinsic aging and photo-aging. Free radicals can be caused by activation of ultraviolet rays, stress, disease state, environmental factors, wounds, and aging. When such state is deepened, the antioxidant defense network existing in the living body is destroyed, And aging. More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

In particular, the oxidation of proteins can cause collagen (collagen), hyaluronic acid, elastin, proteoglycan, fibronectin, and the like that are severely hyperinflammatory reactions and skin elasticity If this gets worse, DNA mutations can lead to mutations, cancer, and immune deficiency. Therefore, it is necessary to protect the cell membrane by destroying the free radicals mediated by free radicals, ultraviolet rays, and inflammation that occur during the metabolism of the body, and to regenerate already damaged cells by vigorous metabolism The skin can quickly recover and maintain healthy skin.

On the other hand, changes in the skin are caused by pigmentation. The factors that determine skin color are basically the partial or total pigmentation such as stain, freckle and tanning due to ultraviolet exposure, and the distribution of acne and scars and keratin Blood circulation, stress, and health. The most important factor among these factors is pigmentation. Melanin, melanoid, carotene and hemoglobin are the most important factors affecting skin color. Among them, melanin is the most important factor affecting the biosynthesis of melanin.

Melanin absorbs or scatters ultraviolet light and plays a major role in preventing damage to the skin from ultraviolet rays. It does not have a specific maximum absorption wavelength and absorbs light in all areas. In addition, it has excellent ability to remove reactive oxygen species, sometimes melanin itself generates active oxygen, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows free radical properties Pray. The biosynthesis of melanin begins with the conversion of tyrosine, an amino acid, into melanosomes of melanocyte-forming melanosomes by tyrosinase to dihydroxyphenylalanine, followed by a series of oxidations to form pheomelanin, It is formed of a polymer of eumelanin. This biosynthesis process is carried out in a special form of melanocyte in the brown intracellular organelle. The melanomas, including melanin granules, migrate from the nucleus to the tip of the dendritic process, and migrate into the cytoplasm by phagocytosis of keratinocytes. It accumulates around the nucleus.

The synthesis of melanin, the number of melanosomes, and the migration to keratinocytes in the periphery are partially affected by hormones and ultraviolet rays, and are primarily affected by genetics. In addition, it is known that tyrosinase expression, melanin synthesis, and intracellular regulatory factors such as cytokine, metal ions such as copper, zinc and iron, and interferon, prostaglandin, and histamine are involved.

(Japanese Patent Laid-open No. 4-9320), hydroquinone (Japanese Patent Laid-Open No. 192062), kojic acid (Japanese Patent Laid-open No. 56-7710), arbutin Has anti-aging activity and is used as a whitening cosmetic. However, since it is low in stability in cosmetic formulations, its use is restricted due to decomposition and coloring, occurrence of odor, efficacy at a living body level, to be. These substances have proved their tyrosinase inhibitory effect, but their effect is low in experiments similar to the actual living body level. Therefore, there is a demand for evaluation of the inhibitory effect by melanoma cell culture similar to a living body level.

Hydroquinone is also defined as a carcinogenic substance and its use in cosmetics is prohibited or restricted. Kojic acid and ascorbic acid are very unstable substances. When a cosmetic containing a small amount of the above components is kept at room temperature for several weeks, browning occurs.

In general, functional substances used in many products related to the skin are effective for the skin and cause skin irritation. Cosmetics containing various raw materials contain substances that can cause irritation or inflammation, so more than 10% of people using personal care products are complaining of side effects on the skin. Recently, Of consumers feel that they are sensitive. In the general consumer consciousness survey, the percentage of respondents who replied that they are sensitive skin is over 30-40%, and this trend continues to increase. Moreover, recent cosmetic development tendency is focused on cosmeceutical which can give a real effect to the skin, and skin side effects due to the use of personal care products such as cosmetics are expected to increase more and more.

Generally, various raw materials are used to make cosmetics, and usually 20 to 50 kinds of raw materials are used. These ingredients are indispensably added in order to preserve, maintain and function cosmetics such as preservatives, surfactants, ultraviolet screening agents, whitening agents, and actually cause skin irritation in some of the products when they are used. These ingredients have been proven to be safe through skin irritation tests, but when applied to products, they can cause skin irritation, erythema and edema, such as inflammation, itching, stinging and burning, And the like. Particularly, the skin is easily damaged due to serious environmental pollution, increase in ultraviolet radiation amount due to destruction of the ozone layer, drying of living space, personal allergic constitution, stress and increase of chemical harmful substances.

In order to solve these various problems, recently, many cosmetics using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

The inventors of the present invention have studied the possibility of application to cosmetics in natural materials that have not been known until now. As a result, Momordica charantia which is a plant and plant was selected and an extract was prepared therefrom. The effect of inhibiting skin damage by ultraviolet rays, Production inhibitory effect, DOPA oxidation inhibitory effect, wrinkle improving effect and antioxidative effect, and as a result, it was found that the efficacy as a cosmetic product can be expected.

The present invention provides a cosmetic composition for preventing skin aging, antioxidation, skin wrinkle improvement or skin whitening comprising an extract of Lilium japonica as an active ingredient.

The lily extract may be a crude extract, a polar solvent-soluble extract or a non-polar solvent-soluble extract. The lady fruit tree extract may be contained in an amount of 1 wt% to 20 wt% based on the cosmetic composition. The cosmetic composition may be a formulation of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack.

The present invention also relates to a method for producing an extract of Latex fruit for use in a cosmetic composition for preventing skin aging, antioxidation, skin wrinkle improvement or skin whitening,

a) extracting the extract from the fruiting body;

b) filtering the lily fruit extract; And

and c) lyophilizing the above-described fruiting body extract.

The step of extracting the extract from the berries may be carried out by heating the berries at a temperature of 100 ° C to 120 ° C for 3 hours to 5 hours.

The step of extracting the extract from the berries may be carried out by immersing berries in ethanol at room temperature for 4 hours to 6 hours.

The fruit extract of the present invention has a melanin production inhibitory effect (Experimental Examples 3-1 and 3-2), a wrinkle improving effect (Experimental Example 4) and an antioxidative effect (Experimental Example 5) (Experimental Example 2) can be used as an effective component (Experimental Example 6) of various functional cosmetic compositions.

Fig. 1 is a graph showing the results of a safety test on cytotoxicity according to Experimental Example 2. Fig.
2 is a graph showing the results of inhibition of tyrosinase activity according to Experimental Example 3-1.
3 is a graph showing the results of DOPA oxidation inhibition test according to Experimental Example 3-2.
FIG. 4 is a graph showing the results of the test for inhibiting the activity of Elastase according to Experimental Example 4. FIG.
5 is a graph showing the results of the antioxidative effect test according to Experimental Example 5.

Unless otherwise defined herein, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Various scientific dictionaries, including the terms contained herein, are well known and available in the art. Although any methods and materials similar or equivalent to those described herein are found to be used in the practice or testing of the present application, some methods and materials have been described. Should not be construed as limiting the invention to the particular methodology, protocols and reagents, as they may be used in various ways in accordance with the context in which those skilled in the art use them.

As used herein, the singular forms include plural objects unless the context clearly dictates otherwise. As used herein, unless otherwise stated, "or" means "and / or ". Furthermore, it is to be understood that other forms, such as " having ", "consisting ", and" consisting "

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

The subject matter provided herein should not be construed as limiting the following embodiments in various aspects or as a reference throughout the specification.

The term "skin whitening" as used herein generally refers to whitening, smoothing, or reducing whiteness of skin tone, skin color and / or skin shade and may include coloring spots, melanin spots, aging spots, sun spots, , Freckles, simple blackness, colorectal keratosis, bruising keratosis, spots, acne scars, hyperpigmentation after inflammation, surplus or nevus.

As used herein, the term "aging" includes the presence of wrinkles and wrinkles, loss of elasticity, uneven skin, spots and blotches. The wrinkles mean fine wrinkles, wrinkles or thick wrinkles, including, but not limited to, wrinkles in the eyes, wrinkles in the forehead and cheeks, wrinkles in the face and wrinkles in the mouth. The loss of elasticity refers to a decrease in elasticity or structural integrity of the skin or tissue, including, but not limited to, diseases, aging, hormonal changes, mechanical trauma, environmental damage. The nonuniform skin refers to the condition of the skin associated with fuzz or speckled pigmentation that can be classified as pigment overdispersion, such as hyperpigmentation after inflammation. The spot refers to a skin condition associated with redness or erythema. The black mold refers to a distinctive pigmentation, for example, a skin condition associated with a small, dense pigmentation zone that can occur on the face as well as the hands.

The term "cosmetics " as used herein refers to aesthetic ingredients or preparations that can preserve, restore, impart, simulate, or enhance the appearance of bodily beauty or enhance beauty or youth, Or the appearance of the skin.

The term " cosmetically effective amount " as used herein means an amount of a physiologically active compound or composition that is sufficient to treat one or more signs of skin aging but is sufficiently low to avoid serious side effects. The effective amount of the compound or composition for cosmetics will depend on the particular condition being treated, the age and physical condition of the user, the severity of the condition to be treated / prevented, the duration of the treatment, the nature of the other treatments, May be varied according to the particular acceptable tint and similar factors.

According to one embodiment, there is provided a cosmetic composition for prevention of skin aging, antioxidation, skin wrinkle improvement or skin whitening comprising an extract of Lilac as an active ingredient.

Momordica charantia, which is used as an active ingredient in the cosmetic composition according to the above embodiment, means an annual plant or fruit of a bamboo plant, having a height of 1 to 3 m and having a shape similar to a scrubber, And climb up with other tendrils and other plants or objects. Domestic seeds sow in April, yellow flowers bloom in June-July, and fruit ripens in September-October. The fruit is long, elliptical, narrow in both ends, with lumpy protrusions, and irregularly ripened in yellowish red, resulting in seeds shrunken in red flesh. It is called Yeoju because its fruit is similar to open space. Yeoju has branched branches with thin branches and thin hairs, and tendons are not branched. Not long ago, Yeoju was cultivated as ornamental plant of the vine fruit, but now it is an income crop as export fruit and vegetables.

In such embodiments, the cosmetic composition may also include, but is not limited to, a preservative component, a moisturizer, a surfactant, organic and inorganic pigments, organic powders, ultraviolet absorbers, bactericides, antioxidants, pH adjusters, alcohols.

The sustaining component may include, but is not limited to, ester based, hydrocarbon based, silicon based, fluorinated, animal and vegetable oils. Examples of the ester-based oil include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isosertyl isostearate , Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, isosertyl isostearate, diethyl sebacinate , Diisopropyl adipate, isoalkyl neopentanoate, tri (capryl, capric acid) glyceryl, tri-2-ethylhexanoate trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid penta Lauric acid, hexyl laurate, decyl myristate, myristyl myristate, myristate, myristyl, stearyl stearate, decyl oleate, ricinoleate But are not limited to, cetyl phosphate, lauric acid, isostearyl isostearate, isostearyl isostearyl isostearate, isostearyl isostearate, isostearyl palmitate, octyl stearate, isostearyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl stearate Ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol , Propylene glycol dicaprylylate, dicapric acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated acid glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyl Dodecylsuccinic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, Polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, octyldecyl lactate, octyldecyl isostearate, polyglycerin oleic acid ester, But are not limited to, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, Propyl stearate, propyl stearate, propyl stearate, stearyl stearate, stearyl stearate, propyl stearate, stearyl stearate, Stearyl hydroxystearate, 12-stearoyl hydroxystearic acid, 12-stearoyl hydroxystearic acid, And aloylhydroxystearic acid is ester-based such as saxophyll. Examples of the hydrocarbonaceous oil include hydrocarbons such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline. Examples of the silicone-based oil include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethylsiloxane methyl cetyloxysiloxane copolymer, dimethylsiloxane methylstearoxysiloxane aerial Alkyl-modified silicone oil, and amino-modified silicone oil. Examples of the fluorine-based oil include perfluoropolyether and the like. Examples of the animal or plant oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Grape seed oil, cottonseed oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, meadow home oil, egg oil, beef fat, , Jojoba oil, canned wax, carnauba wax, liquid lanolin, and hardened castor oil.

The humectant may include, but is not limited to, a water-soluble low molecular weight humectant, a liposoluble molecular moisturizer, a water-soluble polymer, and a liposoluble polymer. Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B , Polypropylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like. Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol, cholesterol ester and the like. Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin . Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

The surfactant may include, but is not limited to, a nonionic surfactant, an anionic surfactant, a cationic surfactant, and a positive surfactant. Examples of the nonionic surfactant include self emulsifying monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid POE.POP (polyoxyethylene.polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, POE.polyester, polyoxyethylene-polyoxypropylene (POE) Lauric acid alkanolamide, alkylamine oxides, hydrogenated soybean phospholipids, and the like. Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylallylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt , Alkylamide phosphate, alkyloyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. . Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethyl bromide Ammonium, benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like. Examples of the amphoteric surfactant include carboxybetaine, amidebetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative, amideamine And a positive surfactant such as a surfactant.

The organic and clay pigments may be selected from the group consisting of silicic acid, anhydrous silicic acid, magnesium silicate, talc, lithite, mica, kaolin, Bengala, clay, bentonite, titanium mica, bismuth oxychloride, zirconium oxide, Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium oxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of an inorganic pigment and an organic pigment, but the present invention is not limited thereto.

The organic powder may be a metal soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; But are not limited to, polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Wherein the ultraviolet absorber is selected from the group consisting of paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethylene glycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, Benzyl, 2-ethoxyethyl methoxycinnamate, octyl methoxycinnamate, dipyrromethoxycinnamic acid mono-2-ethylhexane glyceryl, isomethanesulfonyl methacrylate, isopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy -5-methylphenyl) benzotriazole, and the like, but are not limited thereto.

Wherein the bactericide is selected from the group consisting of hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethyl phenol, azulene, salicylic acid, But are not limited to, photosensitizer 301, mononitro and eicol sodium, undecylenic acid, and the like.

The antioxidant may include, but is not limited to, butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

The pH adjuster may include, but is not limited to, citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

The alcohol may include, but is not limited to, higher alcohols such as cetyl alcohol.

In this embodiment, the fruity fruit extract may be included in an amount of 1 wt% to 20 wt%, or 5 wt% to 10 wt%, based on the total weight of the composition. If the content of the extract is less than 1% by weight, the effect of improving the skin is not exhibited. If the amount of the extract is more than 20% by weight, the effect of improving the skin against the increase of the content is insignificant and not economical. However, it may be increased or decreased according to the needs of the patient, and may be appropriately increased or decreased depending on the condition of the skin, the progress of aging, and the like.

In this embodiment, the fruity fruit extract may be a crude extract, a polar solvent-soluble extract or a non-polar solvent-soluble extract.

The crude extract may be an extract which is soluble in water, alcohol, lower alcohol having 1 to 4 carbon atoms, acetone, chloroform, methylene chloride, hexane or a mixed solvent thereof, including purified water. The polar solvent-soluble extract may be an extract which is soluble in water, alcohol, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The non-polar solvent-soluble extract may be an extract which is soluble in a non-polar solvent containing ethyl acetate, chloroform, hexane and dichloromethane.

In such embodiments, the cosmetic composition may be prepared in any of the formulations conventionally manufactured in the art including, for example, lotions, skins, lotions, nutritional lotions, nutritional creams, massage creams, essences, But is not limited thereto.

When the formulations of the cosmetic composition are pastes, creams or gels, animal fibers, plant fibers, waxes, paraffin, starch, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide .

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition is in the form of a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition is an interface-active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

According to another embodiment, there is provided a method for producing a fruity fruit extract for use in a cosmetic composition for preventing skin aging, antioxidation, skin wrinkle improvement or skin whitening. The method comprising:

a) extracting the extract from the fruiting body;

b) filtering the lily fruit extract; And

and c) lyophilizing the above-described fruiting extract.

In the above embodiment, the lily fruit extract is prepared by pulverizing dried lady fruit with a pulverizer, and then adding water, alcohol, lower alcohol having 1 to 4 carbon atoms, acetone, chloroform, methylene chloride, hexane, Hydrothermal extraction, ethanol extraction, ultrasonic extraction or reflux cooling extraction method at room temperature to 120 ° C for 1 hour to 12 hours, or 3 hours to 5 hours, or 4 hours, A first step of obtaining a crude extract; A second step of filtering the crude extract; And a third step of lyophilizing the crude extract.

In this embodiment, the fruity fruit extract may be a polar solvent or a non-polar soluble extract. The water extract is added to the crude extract and then fractionated using n-hexane, methylene chloride, ethyl acetate and butanol to prepare a non-polar solvent such as n-hexane, chloroform, ethyl acetate and the like. A non-polar solvent-soluble extractable fraction; And polar solvent-soluble extract fractions soluble in polar solvents such as butanol and water.

The fruit extract of the present invention has a melanin production inhibitory effect (Experimental Examples 3-1 and 3-2), a wrinkle improving effect (Experimental Example 4) and an antioxidative effect (Experimental Example 5) (Experimental Example 2) and can be used as an effective component (Experimental Example 6) of various functional cosmetics.

Hereinafter, various embodiments are shown to facilitate understanding of the present invention. The following examples are provided only for the purpose of easier understanding of the present invention, and the scope of protection of the present invention is not limited to the following examples.

<Examples>

Example 1. Preparation of Hot Water Extract of Yeoju

Forty grams of dried mackerel, 400 g of mackerel, containing all of the husks, seeds and seeds of dried mackerel were added to 800 mL of purified water, heated at about 100 ° C for 4 hours, and subjected to hot water extraction once to a weight of 40%. The extract was filtered using a 200 mesh (0.75 mu m) filter paper and lyophilized. As a result, Yeoju hot water extract having a pH of 5.2 and a specific gravity of 1.1 was obtained.

Example 2 Preparation of Ethanol Extract of Yejoo

800 g of 70% ethanol was added to 400 g of Yeast, which contained all of the husks, seeds and seeds of dried lady fruit, and the resultant was dipped for 4 hours at room temperature of 25 ° C and extracted once with ethanol so as to have a weight of 110%. The extract was filtered using a 200 mesh (0.75 mu m) filter paper and lyophilized. As a result, Yeoju ethanol extract having pale green, pH 4.7 and specific gravity of 1.1 was obtained.

<Experimental Example>

Experimental Example 1. Measurement of total polyphenol content

The total polyphenol contents of the rumen extracts obtained from Examples 1 and 2 were analyzed as follows. Folin-Denis reagent, Na ₂ CO ₃ , and tannic acid were purchased from Sigma Chemical Co. (USA). Absorbance was measured by ELISA reader (PowerWave XS2, BioTek, USA) .

Total polyphenol content was measured by Folin-Denis method. 1 mg of hot water and ethanol extract was dissolved in 1 ml of distilled water, 1 ml of Folin-Denis reagent was added, and the mixture was mixed in a 27 ° C water bath. After 5 minutes, 1 ml of a saturated solution of Na ₂ CO ₃ was added, and the mixture was allowed to stand at room temperature for 1 hour and then absorbance was measured at 725 nm. At this time, a standard curve using tannic acid was prepared so that final concentrations were 0, 12.25, 25, 50, and 100 μg / ml. The results are shown in Table 1 below. Polyphenol is made up of a wide variety of ingredients, including aromatics, and is known to exert antioxidative and UV-blocking effects in plants. The hydrothermal and ethanol extracts of Lyons were similar in polyphenol content and relatively higher than other plants.

Experimental Example  2. Safety test for cytotoxicity

The safety of the extracts obtained from Examples 1 and 2 was measured by MTT using a B16F10 mouse melanoma cell. This assay is a modified version of Mosmann's method. The MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-dihydro- diphenyl-tetrazolium bromide) is reduced to dark blue, non-aqueous formazan.

Cells cultured in DMEM medium were added to 96 wells of cells / ml together with the culture medium for 24 hours. The cells were adhered, replaced with fresh medium containing the sample, and cultured again for 24 hours. 20 μl of MTT was added thereto and the amount of formazan produced in purple was quantified by dissolving formazan formed in ₂ % culture in a 5% CO ₂ incubator (Sanyo, Japan) at 37 ° C in 200 μl DMSO . The amount of formazan produced is proportional to the number of viable cells, so the survival rate of the cells can be measured.

The cell line used for measuring cell viability was B16F10 mouse melanoma cell, which was purchased from Korean Cell Line Bank. Cell viability assay reagent is Dimethylsulphoxide (DMSO) is a sigma chemical Co. was purchased from (USA) 37 ℃, in _{5% CO 2 incubator 10% Fetal} Bovine Serum (FBS, JRS Inc.), 100 U / mL penicillin and 100 ㎍ Dulbecco's modified Eagle's medium (DMEM, Lonza) was used. The results are shown in Fig.

As can be seen from Fig. 1, when the hot water extract of Yeoju and the ethanol extract of Yeoju were contained in an amount of 10-100% of the total weight, no cytotoxicity was observed. The most important characteristic as the cosmetic material is excellent safety, and it has been confirmed that the hot water and ethanol extract of Yeoju do not show cytotoxicity even at a high concentration of 1000 ug / ml, and thus it can be used as a cosmetic material in a very high content.

Experimental Example 3. Whitening effect test

The whitening effect of the samples obtained from the above Examples 1 and 2 was measured by the following method. Mushroom-Tyrosinase, L-tyrosine and 3,4-Dihydroxy-L-phenyl-alanine (DOPA) reagents were purchased from Sigma Chemical Co. (USA) and Albutin was purchased from TCI Respectively.

Experimental Example 3-1. Tyrosinase activity inhibition experiment

Tyrosinase is an enzyme that helps the production of melanin by stimulating the oxidation process of tyrosine in vivo. This experiment applies a method of inhibiting the function of the enzyme and measuring the degree of inhibition of tyrosine oxidation to form melanin, a black polymer. 180 μl of 0.1 M sodium phosphate buffer (pH 6.8), 80 μl of diluted each concentration of extracts from Examples 1 and 2 and 20 μl of mushroom tyrosinase were mixed with 20 μl of 1.5 mM L-Tyrosine, followed by reaction at 37 ° C. for 10 minutes, The absorbance was measured. The inhibition rate was measured as follows, and the results are shown in Fig.

Inhibition ratio (%) = [1 - {(Exp. - Blank) / Control}] x 100

As can be seen from FIG. 2, the hot-water extract of Yeoju and the ethanol extract of Yeojoo showed 49% and 32% tyrosinase inhibitory effect at a concentration of 500 ug / ml, respectively. This value is somewhat lower than the control group, arbutin, but it is confirmed that it has sufficient competitiveness in comparison with whitening functional materials derived from various natural products developed in the market.

Experimental Example 3-2. DOPA oxidation inhibition test

20 μl of sample solution and 20 μl of mushroom tyrosinase were mixed with 240 μl of 0.1 M potassium phosphate buffer (pH 7.0), and the mixture was reacted at 37 ° C for 6 minutes. Then, 20 μl of a substrate solution in which 2 mM L-DOPA was dissolved was added, and DOPA chrome produced after reaction at 25 ° C. for 1 minute was measured at an absorbance of 475 nm and expressed as a percentage (%). Respectively. The results are shown in Fig.

As can be seen from FIG. 3, the hot water extract of Yeoju and the ethanol extract of Yeoju showed 41% and 54% DOPA oxidation inhibitory effect at 500 ug / ml, respectively. Especially, the ethanol extracts of Yeoju fruit were significantly more effective than the control.

Experimental Example 4. Wrinkle-improving effect test

The wrinkle-improving effect of the samples obtained from the above Examples 1 and 2 was tested by the following elastase inhibiting effect. Elastase is an enzyme that decomposes elastin, which resides in the skin's dermis and maintains skin elasticity. The elastase inhibitory activity of the extract of the present invention was measured by using adenosine, which is known as an inhibitor of elastase, as a control.

20 μl of 1 mM Succ-Ala-Ala-Ala-p-nitroanilide was added to 180 μl of 0.2 M Tris-HCl (pH 8.0) and 80 μl of the yeast extract of Examples 1 and 2 were mixed. To this was added 20 [mu] l of 2.5 U porcine pancreas solution. Enzyme activation was carried out at 25 ° C for 10 minutes. After the reaction, absorbance values of the control group and each sample were measured at 410 nm using an ELISA reader. 100% extracts were tested to compare wrinkle-improving effects. Adenosine was used as a positive control. The results are shown in Fig.

As can be seen from FIG. 4, the hot-water extract of Yeoju and the ethanol extract of Yeojoo showed 37% and 51% inhibition of elastase at a concentration of 500 ug / ml, respectively. In particular, the ethanol extract showed an elastase inhibitory effect similar to that of adenosine when the concentration was increased as compared with the control adenosine. Therefore, it was found that the ethanol extract of Yeoju fruit is excellent as a natural wrinkle improving material for cosmetics.

Experimental Example 5. Antioxidant Effect Test

The antioxidative effects of the extracts obtained from Examples 1 and 2 were measured by the DPPH free oxygen scavenging test. DPPH is a very stable free radical, a violet compound that exhibits characteristic light absorption at 517 nm. DPPH is very stable in organic solvents such as alcohol, and since it is decolorized by the proton-radical scavenger during the antioxidant mechanism, the antioxidant activity can be easily observed with the naked eye, and thus it is widely used to search for antioxidants from various natural materials. ⁾ Active oxygen called harmful oxygen attacks unsaturated fatty acid which is a constituent of cellular membrane and causes lipid peroxidation reaction and accumulates lipid peroxidation in the body, resulting in deterioration of vital function and at the same time causing pigmentation, aging and diseases of adult diseases .

The degree of free radical scavenging activity of antioxidants depends on the hydrogen donating ability of antioxidants. During visual observation, purple DPPH reacts with an antioxidant with excellent free radical scavenging activity and is reduced to yellow. 100 μl DPPH (α, α-diphenyl-β-picrylhydrazyl, Sigma, USA) was reacted with 200 μl of the extract at room temperature for 20 minutes. The absorbance at 517 nm was measured with an ELISA leader (Synergy HT, BIOTEK, USA). Vitamin C was used as a positive control. The results are shown in Fig.

As can be seen from FIG. 5, the hot water extract of Yeoju and the ethanol extract of Yeoju showed antioxidative effects of 37% and 60% at a concentration of 500 ug / ml, respectively.

Experimental Example 6. Stability Test of Cosmetic Formulation

The stability of cosmetic formulations, i.e., liquid preparations and lotions, prepared using the extracts of the present invention from the above Examples 1 and 2, was tested. The composition of the liquid preparation includes 1% of extract, 12.5% of coconut oil, 12.5% of sweet almond oil, 5.5% of wax, and 5% of yujoo extract, 40% of rose water, 2% of hyaluronic acid, 3% of glycerin and 50% of purified water. , Preservatives (3.5%) and purified water (65%).

In the stability test, changes of pH and viscosity were measured while keeping the liquid and lotion at 25 ℃ for 28 days, respectively, and the changes of phase separation and external appearance were observed. Viscosity was measured by Brookfield viscometer, S63 spindle for liquid, and S64 spindle for lotion at 60 rpm for 30 seconds. The results are shown in Tables 2 and 3.

As can be seen from the above Table 2, the stability test using the hot water extract of Yeoju fruit showed that the appearance of the lotion was white and that of the liquid agent was transparent and no phase separation was observed in both formulations. As a result of the test of pH and viscosity, the stability was maintained for 28 days, and it was confirmed that the hot water extract of Yeoju was relatively excellent in fusion with other materials as a cosmetic material.

As can be seen from the above Table 3, the stability test using the ethanol extract of Lycoris spp. Showed that the appearance of the lotion and the liquid agent remained white and transparent, and no phase separation was observed. As a result of pH and viscosity test, the stability was maintained for 28 days, and the ethanol extract of Yeoju was found to be relatively good in fusion with other materials as a cosmetic material.

The hot-water extract and the ethanol extract of the mulberry juice according to the present invention have not only a very low cytotoxicity, but also exhibit excellent whitening, wrinkle-reducing and antioxidative effects and thus have high potential as a natural functional cosmetic material. Therefore, in many cosmetics, the functional material is not used as a single ingredient but is used in combination with various extracts. Thus, the extract of Yeoju fruit is expected to be incorporated into cosmetics for various purposes.

Claims (10)

A cosmetic composition for preventing skin aging, antioxidation, skin wrinkle improvement or skin whitening comprising an extract of Yejoo as an active ingredient.
The method according to claim 1,
Wherein the mallow fruit extract is a crude extract, a polar solvent-soluble extract, or a non-polar solvent-soluble extract.
3. The method of claim 2,
Wherein the crude extract is an extract obtained by adding water, purified water, alcohol, lower alcohol having 1 to 4 carbon atoms, acetone, chloroform, methylene chloride, hexane or a mixed solvent thereof.
3. The method of claim 2,
Wherein the polar solvent-soluble extract is an extract which is soluble in water, alcohol, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
3. The method of claim 2,
Wherein the non-polar solvent-soluble extract is an extract which is soluble in a non-polar solvent containing ethyl acetate, chloroform, hexane and dichloromethane.
The method according to claim 1,
Wherein the extract is contained in an amount of 1 to 20% by weight based on the cosmetic composition.
The method according to claim 1,
Wherein the cosmetic composition is a formulation of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack.
a) extracting the extract from the fruiting body;
b) filtering the lily fruit extract; And
and c) lyophilizing the extract of lily of the valley. The method for producing a lily of the world for use in a cosmetic composition for preventing skin aging, antioxidation, skin wrinkle improvement or skin whitening.
9. The method of claim 8,
Wherein the step of extracting the extract from the berries comprises heating the berries at a temperature of 100 ° C to 120 ° C for 3 hours to 5 hours.
9. The method of claim 8,
Wherein the step of extracting the extract from the berries comprises dipping the berries at room temperature in ethanol for 4 to 6 hours.

Images