KR100715485B1 - Cosmetic composition comprising the root extract of nymphaea tetragona having skin whitening and anti-aging activity - Google Patents

Abstract

The present invention is a water lily having wrinkle improvement, elasticity enhancement, antioxidant and whitening effect (Nymphaea tetragonaThe present invention relates to a cosmetic composition containing the extract, and more particularly, to improve wrinkles, enhance elasticity, antioxidant and whitening, using the water lily root extract having free radical scavenging activity, elastase inhibitory effect and tyrosinase activity inhibitory effect. It relates to a cosmetic composition.

Water lily, wrinkle improvement, elasticity enhancement, antioxidant, whitening, cosmetic composition

Description

Cosmetic composition containing water lily root extract having skin whitening and anti-aging effect {Cosmetic composition comprising the root extract of Nymphaea tetragona having skin whitening and anti-aging activity.}

The present invention relates to a cosmetic composition containing the water lily root extract as an active ingredient.

Nymphaea tetragona ) is distributed in Korea (sub-central), Japan, eastern Siberia, China, and India. It is a perennial aquatic plant whose leaves are horseshoe shaped and white flowers bloom in summer. Flavonoids on the leaves, alkaloid nymphane, ellagic acid on the rhizome and delphinidin-3-glycoside on the petals. In the private sector, the leaves are used alone, the flower extract is used as a heat lowering drug, the rhizome is used for bronchial asthma and lung diseases. Flowers and outposts are used for sedatives, blood-feeding drugs, and diarrhea ulcers, as well as jaundice and kidney disease.

In order to solve the root cause of human aging, modern science is conducting its research in various ways. In particular, as life improves, there is an increasing interest in preventing not only internal aging of the body but also aging issued to external parts. The optimal study of external aging is skin, and many studies using skin play a large role in identifying human aging (GJ Fisher, The pathophysiology of photoaging of the skin.Cutis ., 75 , pp5- 8, 2005)

The aging of the skin can be divided into two types: one is endogenous aging (chronologic aging) and the structure and function of the skin is continuously deteriorated with the organs over time and the other is exogenous aging (Photoaging) It is divided into changes in skin tissue due to prolonged exposure to sunlight. This aging process leads to dry skin, wrinkles and loss of skin elasticity. The most important issues addressed in the study of skin aging are free radicals and reactive oxygen species. These naturally accentuated made in vivo, but is is generated by the contact, such as the sun light exposure and various chemicals (BA Jurkiewicz and GR Buettner, Ultraviolet light-induced free radical formation in skin:.. An electron paramagnetic resonance study Photochem Photobiol ., 59, pp 1-4, 1994) The generated free electrons or reactive oxygen species exert oxidative stress on skin cells, and these substances are thought to be the causative agents of melanin and wrinkles (Tomita, Y. and Seiji). , M. Inactivation mechanism of tyrosinase in mouse melanoma. J. Dermatol., 4, pp245-249, 1977), by exposure to free radicals or the like of polymer matrix to maintain the elasticity in the skin (collagen, hyaluronic acid, elastin), these substrates are Sequential cleavage was found (M. Yaar and BA Gilchrest, Aging versus photoaging: postulated mechanisms and effectors, J. Investig. Dermatol. Symp. Proc., 3, pp47-51, 1998).

On the other hand, pigmentation in the skin is caused by melanogenesis or proliferation of melanocytes, which can be suppressed in various ways. Commonly used methods include various pathways such as inhibition of tyrosinase activity, an important enzyme of melanin synthesis, decreased function of melanocytes, reduction of melanin through inhibition of automatic oxidation, and inhibition of inflammatory reactions such as erythema. have.

As a result, in order to prevent skin aging, it is necessary to be able to suppress the generation of excess reactive oxygen species and to efficiently remove the generated free radicals, and to inhibit the enzymes causing wrinkles on the skin and to inhibit the activity of the enzymes that proceed with pigmentation (Goihman-Yahr, M., Skin aging and photoaging: an outlook. Clin . Dermatol . 14, pp153-160, 1996).

In order to block the oxidation, delay the aging caused by the oxidation, and to find the ingredients for inhibiting the pigmentation of the skin, the natural natural plants were searched for excellent antioxidant effects, wrinkle improvement and The present invention was completed by confirming that not only the elasticity promoting effect but also the whitening effect, and also by using natural plants can solve allergies and side effects caused by synthetic raw materials, safety and stability.

An object of the present invention is to improve the wrinkles, enhance elasticity, antioxidant and whitening effect ( Nymphaea Tetragona ) The purpose is to provide a cosmetic composition comprising a root extract.

In order to achieve the above object, the present invention is a water lily ( Nymphaea) having wrinkle improvement, elasticity enhancement, antioxidant and whitening effect Tetragona ) Provides a cosmetic composition containing the root extract as an active ingredient.

The extract is a group consisting of C ₁ to C ₄ lower alcohol, butylene glycol, propylene glycol, glycerin, hydrous ethanol, hydrous butylene glycol, hydrous propylene glycol, hexane, ethyl acetate, including purified water, methanol, ethanol, and the like. It comprises a cosmetic composition extracted with one or more solvents selected from.

In addition, the extract includes a cosmetic composition, characterized in that the extract by 1-20 days or immersed for 2-20 hours at 40-100 ℃.

Hereinafter, the present invention will be described in detail.

The process of obtaining the water lily root extract of the present invention is the first step of extracting the active ingredient using the extract solvent in the water lily root, and to increase the content of the active ingredient extracted in step 1 simply concentrated under reduced pressure or using an organic solvent in the aqueous layer After fractionation of the water-insoluble layer and concentrated under reduced pressure.

More specifically, the root of the water lily is one selected from the group consisting of 1 to 20 times the volume of water, methanol, ethanol, butylene glycol, propylene glycol, glycerin, hydrous ethanol, hydrous butylene glycol, and hydrous propylene glycol. The above solvent is extracted as an extraction solvent. As the extraction method, a cooling condenser is installed to prevent evaporation of the solvent, and the extraction is performed by heating at 0 to 200 ° C, preferably 40 to 95 ° C for 1 to 40 hours, preferably 4 to 20 hours, or 0 to 100 A method of extracting the active ingredient by depositing at 1 ° C., preferably 5 to 40 ° C. for 1 hour to 30 days, preferably 1 to 15 days can be used. In addition, an extraction method obtained by extracting 1-20 times by volume of hydrous ethanol and treating hexane, ethyl acetate, butanol, and water stepwise may be used. The water lily root extract thus extracted is concentrated under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser to obtain the water lily root extract.

The water lily root extract can be used in 0.001 to 90% by weight, preferably 0.01 to 10% by weight, more preferably 0.01 to 5% by weight based on the total weight of the cosmetic composition.

Water lily root extract of the present invention can be used in a variety of cosmetics and cleansing agents for wrinkle improvement, elasticity enhancement, antioxidant and whitening effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantotenylethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other ingredients that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments and flavorings. , Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol phthalic acid, neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl tritridecyl acid, glyceryl triisopalmitinate, glyceryl triisosulfate, octyldode neopentane Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight percentage with respect to a total weight, More preferably 0.01-3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example  1. Preparation of Water Lily Root Extract

1-1. Example  1, manufacture

1 kg of water lily roots were put in 10 L of 95% ethanol, extracted by stirring at room temperature for 5 days, filtered through a 250 mesh filter cloth, left to mature at 4 ° C. for 7 days, and filtered through Whatman filter paper to extract 8.8 L (solid content 35.8 g). Got.

1-2. Example  Manufacture of 2 ~ 17

Using the solvent of Table 1 below and extracting in the same manner as in Example 1 and the results are shown in Table 1 below.

Solvent Extract (L) Solids (g) Example 2. water 8.9 19.84 Example 3. Methanol 9.3 13.65 Example 4. Butylene glycol 8.7 12.10 Example 5. Propylene glycol 8.7 19.52 Example 6. glycerin 8.8 7.39 Example 7. Water Butylene Glycol (30 w / w%) 8.7 6.25 Example 8. Hydrous Propylene Glycol (30 w / w%) 9.8 15.4 Example 9. Hydrous ethanol (30 w / w%) 8.9 28.34 Example 10. Hydrous ethanol (70 w / w%) 8.8 32.11

1-3. Example  Manufacture of 11

1 kg of water lily roots were placed in 10 L of hydrous ethanol (95w / w%), extracted by heating in a water bath at 70 ° C. equipped with a cooling condenser for 5 hours, filtered through a 250 mesh filter cloth, and left to mature at 4 ° C. for 7 days. Then, the resultant was filtered through Whatman filter paper to obtain 8.6 L (solid content 23.4 g) of the extract.

1-4. Example  Manufacture of 12-15

Using the solvent of Table 2 below and extracting in the same manner as in Example 11 and the results are shown in Table 2 below.

menstruum Extract (L) Solids (g) Example 12. water 8.8 24.32 Example 13. Methanol 9.0 20.22 Example 14. Hydrous ethanol (30 w / w%) 9.0 35.23 Example 15. Hydrous ethanol (70 w / w%) 8.9 40.10

1-5. Example  Manufacture of 16

20 g of the extract powder obtained in Example 10 was dispersed in 400 g of water, and 400 g of n-hexane was added thereto, followed by shaking extraction to divide the upper and lower layers, and recover the upper layer (n-hexane). The extract obtained by performing this method three times is filtered and concentrated under reduced pressure. 2.1 g of extract powder was obtained.

1-6. Example  Manufacture of 17

400 g of ethyl acetate was added to the lower layer (water layer) obtained in Example 16, followed by shaking extraction. The mixture was divided into an upper layer and a lower layer, and the upper layer (ethyl acetate layer) was recovered. The extract obtained by performing this method three times is filtered and concentrated under reduced pressure. 7.18 g of extract powder were obtained.

1-7. Example  Manufacture of 18

400 g of n-butanol was added to the lower layer (water layer) obtained in Example 17, followed by shaking extraction to divide the upper layer and the lower layer, and recover the upper layer (butanol layer). The extract obtained by performing this method three times is filtered and concentrated under reduced pressure. 8.31 g of extract powder was obtained.

1-8. Example  Manufacture of 19

The water layer obtained in Example 18 was concentrated under reduced pressure to obtain 1.42 g of an extract powder.

Experimental Example  One. free Radical  Efficacy Experiment

As a sample of Examples 1 to 19, a stable 1,1-diphenyl-2-picrylhydrazyl radical (1,1-Diphenyl-2-picrylhydrazyl radical) was used. (MS Blois, Antioxidant determinations by the use of a stable free radical, Nature , 29, pp1199-1200, 1958) .Put the sample in 1 ml of 0.2 mM diphenyl-2-picrylhydrazyl (DPPH) solution at each concentration. After adding mL and reacting at room temperature for 10 minutes, the maximum absorbance at 525 nm is measured to determine the free radical scavenging effect (%). Free radical scavenging ability was calculated as in Equation 1 below.

Superoxide radical scavenging activity SC ₅₀ is the concentration (μg / ml) of the extract required for 50% scavenging of superoxide radicals, and the results are shown in Table 3.

Free radical scavenging effect Example SC ₅₀ (μg / ml) Example 1 6.5 Example 2 25.5 Example 3 12.3 Example 4 11.6 Example 5 14.3 Example 6 12.9 Example 7 21.2 Example 8 10.2 Example 9 6.5 Example 10 4.7 Example 11 14.3 Example 12 9.4 Example 13 8.2 Example 14 6.5 Example 15 5.5 Example 16 5.1 Example 17 0.8 Example 18 6.4 Example 19 8.9

Experimental Example  2. Elastase  Inhibitory effect experiment

The inhibitory effect of elastase activity on the water lily extracts of Examples 1 to 19 was measured. Substrate solution containing 8.8 mM of the Elastase substrate Succ-Ala-Ala-A-p-nitroanilide standard in 60 µl of the buffer adjusted with 0.267 M Tris solution with 0.267 M hydrochloric acid (HCl) to pH 8.0. 100 μl of the samples prepared by diluting the extracts obtained in Examples 1 to 19 to a predetermined concentration in purified water were mixed and mixed with each other. The porcine pancreatic elastase standard was 10 μg / ml. After adding 20 μl of the enzyme solution, the mixture was reacted at 25 ° C. for 15 minutes, and the absorbance B was measured at 410 nm for the amount of para-nitroaniline produced.

In addition, 100 μl of the sample prepared by adding 20 μl of substrate solution, 20 μl of purified water, 20 μl of purified water, and purified water of a constant concentration of the extract obtained in Examples 1 to 23 was added to 60 μl of the same buffer as described above. After that, the absorbance C was measured as a control.

In addition, the absorbance A was measured using 100 μl of purified water instead of the sample as the test solution, and the absorbance A was measured using 120 μl of purified water instead of the enzyme solution and the test solution. Was taken as the color correction liquid and the absorbance D was measured to obtain an elastase inhibition rate (%) as shown in Equation 2 below. Elastase inhibitory activity IC ₅₀ for each example was expressed as the concentration of extract (μg / ml) required to inhibit the activity of elastase by 50%, and the results are shown in Table 4.

Elastase Inhibitory Effect Example IC ₅₀ (Μg / ml) Example 1 123.2 Example 2 95.0 Example 3 88.3 Example 4 120.4 Example 5 110.0 Example 6 103.3 Example 7 98.5 Example 8 88.2 Example 9 134.5 Example 10 70.4 Example 11 101.2 Example 12 90.2 Example 13 95.5 Example 14 110.3 Example 15 78.4 Example 16 138.3 Example 17 77.3 Example 18 87.3 Example 19 89.5

Experimental Example  3. Tyrosinase activity of  Inhibitory effect ( Tyrosinase inhibitory activity )

The inhibitory activity of tyrosinase was measured for the water lily extracts of Examples 1 to 28. Mix 1.0 ml of 0.1 M potassium phosphate buffer (pH 6.8), 1.0 ml of 0.3 mg / ml L-tyrosine aqueous solution, 0.1 ml of 1250 unit / ml mushroom tyrosinase After this, 0.2 ml of the sample solution was added thereto according to the concentration, and the enzyme reaction was performed at 37 ° C. for 10 minutes. The absorbance of the reaction solution was measured at 480 nm, and the enzyme inhibitory activity of the sample was measured according to Equation 3 below.

Tyrosinase inhibitory activity for each Example IC ₅₀ was expressed as the concentration of extract (μg / ㎖) required to inhibit the tyrosinase activity 50%, the results are shown in Table 5.

Example IC ₅₀ (μg / ml) Example 1 1250 Example 2 980 Example 3 730 Example 4 850 Example 5 960 Example 6 650 Example 7 740 Example 8 990 Example 9 800 Example 10 500 Example 11 920 Example 12 1540 Example 13 850 Example 14 870 Example 15 880 Example 16 900 Example 17 440 Example 18 890 Example 19 717

Experimental Example 4 . B16 Melanoma ( melanoma ) Measurement of Melanin Biosynthesis Inhibitory Effect Using Cells

In order to measure the whitening effect in the cells of the water lily extracts of Examples 1 to 19, the melanogenesis inhibitory effect using B16 melanoma cells was measured by modifying the method of Maeda and Fukuda (K Maeda, and M. Fukuda, In vitro effectiveness of several whitening cosmetic components in human melanocytes, J. Soc . Cosmetic Chem . , 42, p361, 1991). Two sets were prepared to simultaneously measure melanin production and cell number, and wells according to all concentrations were prepared as triplets. B16 melanoma cells (ATCC CRL6323) were inoculated at a density of 1 × 10 ⁵ cells in DMEM (Dulbecco's modified Eagle's Medium) with 10% bovine serum and 1% antibiotic and incubated at 5% CO ₂ , 37 ° C. for one day. Thereafter, the mixture was changed to DMEM 10% medium treated with α-MSH (0.34 / ml, Sigma), and the active ingredients separated from the samples were incubated for 3 days by treating the wells by concentration. After 3 days, the set to measure the amount of melanin collected the medium in a 15 ml flask tube and the cells were detached by trypsin treatment and the medium was transferred to the collected tube. The supernatant was discarded by centrifugation at 3,000 rpm for 30 minutes, treated with 250 μl of 1N NaOH, and then centrifuged lightly for 10 minutes in boiling water. After 10 minutes of sonication, the melanin was completely dissolved, and the absorbance was measured at 475 nm. The cell number measuring set was treated with 1 ml of 0.33 mg / ml MTT (Sigma M5655) for 4 hours at 37 ° C. After that, MTT was removed and 1 ml of DMSO was added to give a 575 nm development. The absorbance was measured at, and the amount of melanin synthesis was measured in comparison with the control that was not treated with the sample (Table 6).

sample Treatment concentration (㎍ / ㎖) Melanin biosynthesis inhibition rate (%) Control 10 0 100 0 Example 1 10 11.1 100 33.2 Example 2 10 15.3 100 56.3 Example 3 10 25.4 100 38.9 Example 4 10 12.2 100 54.3 Example 5 10 16.3 100 33.1 Example 6 10 33.2 100 64.2 Example 7 10 19.4 100 56.8 Example 8 10 34.7 100 44.6 Example 9 10 16.4 100 36.6 Example 10 10 27.4 100 67.4 Example 11 10 22.3 100 45.9 Example 12 10 11.3 100 56.0 Example 13 10 13.2 100 25.4 Example 14 10 9.4 100 24.3 Example 15 10 19.3 100 56.0 Example 16 10 11.2 100 45.4 Example 17 10 28.5 100 95.2 Example 18 10 31.8 100 89.1 Example 19 10 31.5 100 49.5

Experimental Example  5. Increased elasticity test on the skin

5-1. Preparation of Sample

The raw materials used in the preparation of the formulation examples and comparative formulations used as samples of the experiment are shown in Table 7 below.

number Raw material Example of formulation (%) Comparative formulation example (%) One Water lily extract of Example 10 10.0 - 2 Squalane 3.50 3.50 3 Cetyl alcohol 3.00 3.00 4 glycerin 3.00 3.00 5 Glyceryl Stearate 2.50 2.50 6 Cetyloctanoate 6.00 6.00 7 Sorbitan Sesquioleate 1.50 1.50 8 Fiji-100 Stearate 1.00 1.00 9 Shea Butter 0.50 0.50 10 Carbomer 0.30 0.30 11 Triethanolamine 0.30 0.30 12 Methylparaben 0.20 0.20 13 Imidazolidinylurea 0.15 0.15 14 Propylparaben 0.10 0.10 15 Combination spices 0.10 0.10 16 Purified water to 100 to 100

The raw material of the above-described content is dissolved in oil by the general emulsification method as the oil phase component, the component dissolved in purified water as the water phase component, the water phase component and the oil phase component at a constant temperature (80 ~ 85 ℃) as a general homo mixer After emulsifying the homogeneous mixture, the thickener (carbomer) was neutralized and the lotion was prepared by cooling, flavoring, water extract addition, and defoaming to prepare a sample for this experiment.

5-2. Experiment method and result

In order to measure the effect of increasing the elasticity of the skin when the lotion of Examples and Comparative Formulations prepared above was applied to the skin, 20 to 55 year old females were treated to 20 owners of normal, oily, dry and complex skin. On the left side of the face, the nutrition lotion of the embodiment is applied, and on the right side of the face, the nutritional lotion of the comparative formulation is applied twice a day, morning and evening, and then skin is applied after 1, 2, 3, and 4 weeks. The elasticity of the skin was measured using a elasticity analyzer (Ballistometer). The measurement results are shown in Table 8 below.

Example of Cosmetics Comparative Formulation Remarks T ₀ T ₁ T ₂ T ₃ T ₄ T ₀ T ₁ T ₂ T ₃ T ₄   Elasticity Spring water 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0   Amplitude first 1.9 3.2 3.6 4.1 3.9 1.9 2.8 2.3 2.6 2.6 second 1.3 2.6 2.8 3.2 3.3 1.6 1.0 1.3 1.5 1.9 third 0.0 1.4 1.6 1.7 1.7 0.0 0.5 0.9 1.0 1.5 fourth 0.0 0.5 0.9 0.9 1.1 0.0 0.0 0.0 0.0 0.0 Average 0.80 1.93 2.23 2.48 2.50 0.88 1.08 1.13 1.27 1.50 T ₀ ; Before use T ₁ ; After 1 week of use T ₃ ; After 3 weeks of use T ₂ ; After 2 weeks of use T ₄ ; After 4 weeks

As can be seen from the above results, it can be seen that the skin elasticity effect is very excellent by the skin condition improvement effect compared to the nutritional lotion of the comparative example prepared without the addition of the nutritional lotion of the embodiment containing the water lily extract according to the present invention. have.

Formulation example  One. Skin  Produce

Raw material content One Water lily extract of Example 10 10.0 2 Propylene glycol 5.00 3 ethanol 3.00 4 Imidazolidinylurea 0.20 5 Allantoin 0.20 6 Citrix Acid 0.20 7 Sodium citrate 0.12 8 Hydrogenated Castor Oil 0.10 9 Methylparaben 0.10 10 Combination spices 0.10 11 Purified water to 100

The water lily extract of Example 10 was mixed to 1% by weight in 50% hydrous butylene glycol, and dissolved in ethanol hydrogenated castor oil, methylparaben, and combination chemicals in purified water in which the remaining ingredients were dissolved. Complete the manufacture.

Formulation example  2. Preparation of Lotion

Raw material content One Water lily extract of Example 10 10.0 2 Squalane 3.50 3 Cetyl alcohol 3.00 4 glycerin 3.00 5 Glyceryl Stearate 2.50 6 Cetyloctanoate 6.00 7 Sorbitan Sesquioleate 1.50 8 Fiji-100 Stearate 1.00 9 Shea Butter 0.50 10 Carbomer 0.30 11 Triethanolamine 0.30 12 Methylparaben 0.20 13 Imidazolidinylurea 0.15 14 Propylparaben 0.10 15 Combination spices 0.10 16 Purified water to 100

The water-soluble component and oil-based component at a constant temperature (80 ~ 85 ℃) are converted to general homomixer by using the raw material of the above-mentioned content as a general oil emulsifying method and the water-soluble component as the water-soluble component. After homogeneous mixing, the thickener (carbomer) is neutralized, followed by cooling, flavoring, water extract addition, and defoaming to complete the manufacturing process.

Formulation example  3. Preparation of Cream

Raw material content One Water lily extract of Example 10 10.0 2 glycerin 6.00 3 Squalane 6.00 4 Isohexadecane 5.50 5 Cetyloctanoate 4.00 6 Glyceryl Stearate 2.50 7 Polysorbate 60 1.00 8 Fiji-100 Stearate 1.00 9 Stearic acid 1.00 10 lanolin 1.00 11 Laureth-12 1.00 12 Cetyl alcohol 0.50 13 Sorbitan cisquioleate 0.30 14 Carbomer 0.30 15 Triethanolamine 0.30 16 Methylparaben 0.20 17 Imidazolidinylurea 0.15 18 Propylparaben 0.10 19 Combination spices 0.10 20 Purified water to 100

The raw material of the above-described content is dissolved in oil by the general emulsification method as the oil phase component, the component dissolved in purified water as the water phase component, the water phase component and the oil phase component at a constant temperature (80 ~ 85 ℃) as a general homo mixer After emulsifying the homogeneous mixture, the thickener (carbomer) is neutralized, and then cooled, flavored, water lily extract is added, and defoaming is completed.

As described above, the present invention relates to a cosmetic composition for improving wrinkles, enhancing elasticity, antioxidant and whitening as an active ingredient of a water lily root extract having a free radical scavenging activity, an elastase inhibitory effect and a tyrosinase activity inhibiting effect. It can be used as an anti-aging and whitening cosmetic containing the extract of the invention.

Claims (5)

Having an anti-wrinkle, elasticity promoting, anti-oxidation and whitening effect lily (Nymphaea tetragona ) Cosmetic composition containing the root extract as an active ingredient. The method of claim 1, wherein the extract is C ₁ to C ₄ lower alcohol, butylene glycol, propylene glycol, glycerin, hydrous ethanol, hydrous butylene glycol, hydrous propylene glycol, hexane, Cosmetic composition extracted with at least one solvent selected from the group consisting of ethyl acetate. The cosmetic composition according to claim 1, wherein the extract is obtained by immersing at 1-40 days at 4-40 ° C or by heating at 20-100 ° C for 2-20 hours. The cosmetic composition according to claim 1, wherein the extract is contained in an amount of 0.01-10% by weight based on the total weight of the composition. According to claim 1, wherein the composition is a cosmetic composition of the skin, nutrition lotion, nutrition cream formulation.