KR100920896B1 - Cosmetic Composition for Skin-Whitening Comprising Nymphaea caerulea and Tetrahydrocurcumin - Google Patents

Abstract

The present invention relates to a cosmetic for skin whitening, and more particularly to a cosmetic composition for skin whitening containing blue lotus (Nymphaea caerulea) and tetrahydrocurcumin (Tetrahydrocurcumin) at the same time. The cosmetic composition of the present invention contains tetrahydro curcumin and blue lotions having a whitening effect at the same time, synergistically increases the whitening effect, and relieves skin irritation that may be caused by cosmetics by blue lotters, thus almost no skin irritation. Indicates the safety of the formulation.

Cosmetics, Whitening, Antioxidants, Blue Lotus, Tetrahydrocurcumin

Description

Cosmetic composition for skin whitening containing blue lotus and tetrahydrocurcumin as an active ingredient {Cosmetic Composition for Skin-Whitening Comprising Nymphaea caerulea and Tetrahydrocurcumin}

The present invention relates to a cosmetic composition, and more particularly, the present invention relates to a cosmetic composition for skin whitening containing blue lotus and tetrahydrocurcumin as an active ingredient.

It is everyone's constant desire to have white, fair skin. In general, the color of human skin, hair and eyes is determined by melanin, carotene and hemoglobin and the like. In particular, melanin is the most important factor for determining the skin color, the skin color is determined according to the amount, nature and distribution of melanin. Human skin and hair pigments protect skin and hair from the harmful effects of sunlight, especially ultraviolet light. For example, people who lack these pigments are very sensitive to sunlight and are prone to burns and have a high probability of developing skin cancer even at a young age. Short wavelength ultraviolet (290-320 nm) and carcinogens form harmful radicals in the skin, such as oxygen radicals, which are known to attack skin cells and age the skin.

In general, the blackening of the human skin is caused by melanin pigment produced by the skin. In normal condition, excessive production of melanin does not occur, but the skin is exposed to external stimuli such as ultraviolet rays, environmental pollution, stress, or hormonal or inflammatory response parameters. Responding to internal factors such as excessive production of melanin pigments, which can't be released out of the skin and remain in the skin, cause pigmentation.

The main function of melanin is to remove these harmful radicals and protect the skin from the damage thereby. Therefore, high levels of melanin means that it has an effective response system that can protect the skin from physical or chemical toxic substances.

Meanwhile, in melanocytes, enzymes such as tyrosinase, tyrosinase related protein (TRP) -1, and TRP-2 are present in the body to form L-tyrosine as a substrate. Acts to produce melanin pigments (Cohen, T., et al. Nucleic Acids Res., 18: 2807-2808 (1990)); (Jackson, I. J., et al., EMBO J., 11: 327-535 (1992)). This enzymatic reaction occurs in the melanocytes, the organelles within the pigment-forming cells, and the newly produced melanin containing melanin is distributed to adjacent keratinocytes through the dendrite of the pigment-forming cells. Causes skin color to appear

Melanin absorbs the light energy of sunlight UV and protects the skin organs under the dermis from UV damage, and it protects the skin from external harmful factors such as trapping harmful oxygen and free radicals generated in the skin living body. Play a role. However, abnormally low production of melanin causes skin lesions such as vitiligo, and on the contrary, damage to the skin may occur if melanin is not completely lost due to excessive synthesis or keratinization of melanin due to sunlight, hormonal changes, inflammation or drugs. In addition to joules, the pigment of the skin is deposited to form blemishes, freckles, and even from these lesions may cause skin cancer. Therefore, in order to prevent such pigmentation phenomenon, it is necessary to inhibit a part of melanogenesis process to reduce melanin production.

Free radicals occur in normal or pathological cell metabolism by external factors such as foreign matter or UV irradiation. When the human body uses oxygen for respiration and combustion, molecular oxygen readily reacts with free radicals, resulting in superoxide (· O ₂ −), hydrogen peroxide (H ₂ O ₂ ), hydroxy radicals (· OH), and peroxy radicals. Produces harmful reactive oxygen species (ROS), such as (ROO-). These ROS act as excellent oxidizing and nitriding agents, damaging major components in the human body, such as lipids, proteins, nucleic acids and DNA, causing physiological disorders and, in severe cases, causing disease and losing life. In addition, reactive oxygen species attack perunsaturated fatty acids, which are components of cell membranes, to cause peroxidation reactions, and the lipid peroxides accumulated in vivo are known to cause aging and various lesions.

In the conventional cosmetic field, inhibitors that inhibit tyrosinase activity, such as kojic acid, arbutin, hydroquinone, vitamin C and its derivatives, licorice extract, etc., have been used as whitening ingredients. . However, they have problems in stability in the formulation and their use is limited.

Accordingly, the present inventors have made diligent efforts to overcome the problems of the conventional skin whitening cosmetic composition, such as weakening the stability, side effects and whitening effect of the cosmetics, it was found that tetrahydrocurcumin has an excellent effect of inhibiting the activity of tyrosinase. The present invention was completed by confirming that the whitening effect was increased and skin irritation was remarkably improved when the tetrahydrocurcumin was used in combination with an appropriate component ratio of Blue Lotus.

Therefore, an object of the present invention is to provide a cosmetic composition for skin whitening containing Blue Lotus and tetrahydrocurcumin as an active ingredient.

As described in detail above, the present invention provides a cosmetic composition for skin whitening containing Blue Lotus and tetrahydrocurcumin as an active ingredient. The cosmetic composition of the present invention synergistically increases the whitening effect by using a combination of Blue Lotus and tetrahydro curcumin as an active ingredient, and is useful as a cosmetic composition for skin whitening by reducing the normal cosmetic skin irritation caused by Blue Lotus and tetrahydro curcumin Can be used.

The present invention relates to a cosmetic composition for skin whitening containing Blue Lotus and tetrahydrocurcumin as an active ingredient.

Blue lotus used in the present invention refers to blue lotus (Nymphaea caerulea) extract. Blue lotus (Nymphaea caerulea) is a perennial herb that belongs to the water lily family and is native to South Africa. It is a wild herbaceous plant with a diameter of about 30 cm and grows on the islands in southern and eastern Asia. Called blue lotus, blue lotus, and dahlia, the plant's name is due to its blue flowering, and it is known to contain anti-aging and antioxidant ingredients and to inhibit free radicals.

In the cosmetic composition of the present invention, the blue lotus extract means obtained by extracting from various organs such as water lilies and leaves, stems, roots, flowers, seeds of plants and plants, and preferably means extracts obtained from leaves or flowers. do.

The Blue Lotus extract of the present invention may be prepared according to conventional methods known in the art, that is, using conventional solvents, under conditions of conventional temperature and pressure.

The Blue Lotus extract can be prepared in various extraction solvents, such as water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol and butanol), acetone, ethyl acetate, chloroform, 1,3-butylene glycol and Obtained as an extraction solvent selected from the group consisting of butyl acetate, preferably obtained by using a suitable mixture of water and ethanol, more preferably using a mixed solution of 3: 7 mixed with water and ethanol It is obtained by. On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained in addition to the above-described extraction solvents, extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The active fraction is also included in the extract of the present invention.

Tetrahydrocurcumin is extracted from Curcuma Longa L., which is a perennial herbaceous plant belonging to Gingeraceae, is native to India, and is cultivated in Taiwan, Indonesia, and Japan. Turmeric leaves are large, 30 ~ 90cm long, 10 ~ 20cm wide, with pointed leaves, triangular base, and blue on the top. Turmeric is mainly used as a medicinal herb and spice. The main ingredients are curcumin derivatives including tetrahydrocurcumin, tumerrone, azulene, and kampfa, which promote liver detoxification, secretion of phlegm and bleeding. It is known to be outstanding.

According to a preferred embodiment of the present invention, the content of the Blue Lotus extract in the composition of the present invention is 0.001-10.0% by weight, preferably 0.01-5.0% by weight, most preferably 0.1-3.0, based on the total weight of the composition. % By weight, the content of tetrahydrocurcumin is 0.03-5.0% by weight, preferably 0.1-5.0% by weight, most preferably 0.5-3.0% by weight relative to the total weight of the composition. In this case, when the content of the blue lotter in the cosmetic composition of the present invention is less than 0.001% by weight, there is a problem in that the tyrosinase activity inhibition effect cannot be expected, and when it exceeds 10.0% by weight, there is no increase in the apparent effect due to the increase in content There is a problem in the stability, the content of the tetrahydrocurcumin is less than 0.03% by weight can not expect the effect of inhibiting tyrosinase activity, if the content exceeds 5.0% by weight does not have a noticeable effect increase with increasing content There is a problem in shape stability.

The cosmetic composition of the present invention achieves a synergic whitening effect by using Blue Lotus and Tetrahydrocurcumin as an active ingredient together, respectively, and the skin irritation is relieved by Blue Lotus, so cosmetic use It can solve the skin irritation problem caused by.

The components included in the cosmetic composition of the present invention include, as an active ingredient, ingredients commonly used in cosmetic compositions in addition to the active ingredients, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and perfumes, And carriers.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art and include, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the dosage form of the present invention is a suspension, water, a liquid diluent such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1 Preparation of Blue Lotus Extract

First, the leaves and flowers of the Blue Lotus are dried and finely crushed to weigh 100 grams (g). 600g of 70% ethanol is added thereto and soaked for 5 days to extract the active ingredient. After extracting for 5 days, the resultant was filtered with a 300 mesh filter cloth, again filtered with a filter paper of Whatman 5, and dried with a rotary vacuum evaporator to obtain a dry weight of 7.5 grams (g). The dried powder was dissolved in 30% 1,3-butylene glycol to prepare a Blue Lotus extract was used in the present invention.

Experimental Example 1 Determination of Tyrosinase Activity Inhibition

Tyrosinase was isolated and purified from mushrooms and purchased from Sigma (United States of America). L-tyrosine (Sigma) was used as a substrate by dissolving in 0.05 M sodium phosphate buffer (pH 6.8) to make a 0.1 mg / ml solution. The test substance was used after dissolving to a suitable concentration in 0.05M sodium phosphate buffer (pH 6.8). Tetrahydrocurcumin was purchased from SABINSA (USA) and used, and Blue Lotus extract was prepared and used according to the method of Preparation Example 1. 0.5 ml of L-tyrosine solution was placed in a test tube, and 0.5 ml of sample solution was added thereto, and the mixture was left to stand at 37 ° C. for 10 minutes. Thereafter, 0.5 ml of 200 unit / ml tyrosinase was added to each sample solution, and the mixture was reacted at 37 ° C for 10 minutes. The test tube containing the reaction solution was placed on ice, quenched to stop the reaction, and the absorbance at wavelength 475 nm was measured with a spectrophotometer. As a control, 0.5 ml of buffer solution was used instead of the sample solution.

The inhibition rate of tyrosinase activity of each sample solution was calculated according to the following equation and the experimental results are shown in Tables 1 to 3 below.

Blue Lotus Extract (µg / mL) Enzyme inhibition rate (%) 5 3.2 10 5.7 20 8.4 50 12.3 100 19.6 200 20.9

Tetrahydrocurcumin (μg / ml) Enzyme Inhibition Rate (%) 5 32.5 10 53.8 20 70.1 50 81.9 100 95.3 200 97.8

Tetrahydrocurcumin (μg / ml) Blue Lotus Extract (µg / mL) Enzyme inhibition rate (%) 10 0 53.8 10 5 65.2 10 10 74.4 10 20 86.3

As can be seen from the results of Tables 1 to 3, tetrahydrocurcumin showed a relatively high inhibitory effect on tyrosinase activity, but the inhibition rate of Blue Lotus enzyme activity was low. However, as shown in Table 3 above, when Blue Lotus and tetrahydrocurcumin were used at the same time, the rate of enzyme activity inhibition was significantly increased compared with the case of using each.

Preparation Example 2 Preparation of Cream Formulation

In order to identify the proper mixing ratio of the Blue Lotus extract and tetrahydrocurcumin in the whitening cosmetic composition, a cream formulation containing Blue Lotus and tetrahydrocurcumin mixed in various mixing ratios was prepared.

Cream formulation containing Blue Lotus and tetrahydrocurcumin Content (% by weight) ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Comparative Example 11 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Blue Lotus Extract - 0.5 One 2 3 4 5 - - - - 3 3 3 3 One 0.5 Tetrahydrocurcumin - - - - - - - 0.5 One 1.5 2 0.5 1.5 One 2 One One Glycerin 4.0, petrolatum 3.5, triethanolamine 2.1, liquid paraffin 5.3, squalane 3.0, beeswax 2.6, tocopheryl acetate 5.4, polysorbate 60 3.2, carboxyvinyl polymer 1.0, sorbitassesquioleate 3.1, trace amounts, preservative traces, Purified water level

Experimental Example 2 Measurement of Whitening Effect

Thirty 26-45 year old females with dark skin color were measured for the whitening effect of each of the cosmetics of Comparative Examples 1-11 and 1-6 prepared in Preparation Example 2. Twelve places of the subject's arms were displayed by 1 cm in width and length, and each of the cosmetics of Preparation Example 2 was applied in the same amount, and the other one was used as a control. Cosmetics were applied twice a day for two months. Skin color (L value) was measured after 2 months using Minolta CR 300 as a measuring device, and the change in skin color was calculated using Equation 2 below and the results are shown in Table 5.

Skin color gradient division Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Comparative Example 11 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 ΔL value 0.53 0.59 0.74 0.78 0.83 0.85 0.86 2.47 2.98 3.53 3.65 3.87 5.46 5.23 5.72 4.96 4.05

As can be seen from the results of Table 5, in the case of Comparative Example 2-7 containing the Blue Lotus extract, ΔL value slightly increased in proportion to the concentration of Blue Lotus compared to Comparative Example 1, which contains nothing, but more than 3.0 wt% There was no change in ΔL values in the concentration (Comparative Example 5-7). In the case of the cream of Comparative Example 8-11 added for each concentration of tetrahydrocurcumin, the ΔL value increased in proportion to the concentration of tetrahydrocurcumin, but the change in ΔL value was not large at a concentration of 1.5 wt% or more. However, in the cream of Example 1-6 prepared by combining Blue Lotus and tetrahydrocurcumin, the change in the ΔL value was much larger than that of the cream containing Blue Lotus and tetrahydrocurcumin, respectively.

Experimental Example 3: Skin Irritation Test

In order to test the skin irritation of each of the cosmetic preparations of Comparative Examples and Examples prepared in Preparation Example 2, a patch test was conducted using a Haye's Test Chamber on 20 healthy adults and men. . After wiping the test site with 70% ethanol and drying, 15 μg or 15 μl of the prepared test substance was placed in a sealed chamber (Finn chamber, 100x10, EPITEST, Finland) in the forearm of the test subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. 1 hour and 24 hours after each removal (1 hour after removal, 24 hours = 24 hours, 48 hours including patch attachment) using a magnifying glass (8MC-150, DAZOR, United States) to observe the test site for erythema and edema Was observed. Skin response was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) (Table 6), the average skin reactivity was calculated by the following equation and the results are shown in Table 7.

Evaluation criteria and score of skin reaction sign score Evaluation standard - 0 No response, normal skin ± 0.5 Faint or light erythema + 1.0 Boundary but weak erythema, edema and papules ++ 2.0 Pronounced erythema, papules and vesicles +++ 3.0 Severe erythema and vesicle formation ++++ 6.0 Cannon formation

Test substance 24 hours 48 hours Average skin reactivity (n = 20)  ± + ++ ± + ++ Comparative Example 1 1 1- One - - 0.48 Comparative Example 2 One - - --- 0.37 Comparative Example 3 One - - --- 0.30 Comparative Example 4 --- --- 0.25 Comparative Example 8 1 1- --- 0.45 Comparative Example 9 2 - - One - - 0.43 Comparative Example 10 2 - - --- 0.40 Comparative Example 11 One - - --- 0.39 Example 1 One - - --- 0.21 Example 2 --- --- 0.18 Example 3 --- --- 0.17 Example 4 --- --- 0.15 Example 5 One - - --- 0.28 Example 6 - One - --- 0.32

As shown in Table 7, the skin reactivity of the cream of Comparative Example 1, which does not contain both Blue Lotus and tetrahydrocurcumin used in the present invention, is very low, so that the skin irritation level of conventional cosmetics is determined by the other ingredients of the cream. It can be seen that it is not shown. However, in the cream of Comparative Example 8-11 containing tetrahydrocurcumin, it can be seen that the irritation intensity was lowered in proportion to the concentration of tetrahydrocurcumin, and the Blue Lotus was also lowered in proportion to the concentration (Comparative Example 2-4). ). However, when the tetrahydrocommunin and Blue Lotus are the same concentration, it can be seen that Blue Lotus's stimulating effect is very high.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (5)

A cosmetic composition for skin whitening containing blue lotus and tetrahydrocurcumin as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the blue rotors are contained in 0.001-10.0% by weight of the total weight of the composition, and the tetrahydrocurcumin is contained in 0.03-5.0% by weight of the total weight of the composition. The method of claim 1, wherein the Blue Lotus is used with a solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol and butyl acetate Cosmetic composition for skin whitening, characterized in that extracted by. The cosmetic composition for skin whitening according to claim 1, wherein the blue lotus and tetrahydrocurcumin have a whitening synergistic effect. A cosmetic method for skin whitening comprising applying the cosmetic composition of claim 1 to human skin.