KR102476670B1 - Composition for skin improvement comprising multiple extract - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a mixed extract of blue lotus and water lily as an active ingredient, and more particularly, a cosmetic composition having excellent effects such as skin whitening, skin elasticity enhancement, wrinkle improvement, skin moisturizing, antioxidant, anti-inflammatory, etc. It is about. The composition of the present invention has excellent anti-inflammatory and antioxidant effects, exhibits the effect of reducing the total amount of melanin, and has excellent collagen synthesis promoting effects and moisturizing effects, so that it can be used as a cosmetic composition for skin whitening, elasticity enhancement or wrinkle improvement, and skin moisturizing. can In addition, the composition containing the mixed extract of blue lotus and water lily of the present invention can be used as a functional food composition and a quasi-drug composition.

Description

Cosmetic composition comprising a mixed extract {Composition for skin improvement comprising multiple extract}

The present invention relates to a cosmetic composition comprising a mixed extract of blue lotus and water lily, and more specifically, excellent skin whitening, skin elasticity promotion, wrinkle improvement, skin moisturizing, antioxidant or anti-inflammatory containing the mixed extract as an active ingredient It relates to a cosmetic composition having an effect. In addition, the present invention relates to a functional food composition and a quasi-drug composition containing a mixed extract of blue lotus and water lily.

Commonly known whitening ingredients include substances that inhibit tyrosinase enzyme activity such as kojic acid or arbutin, hydroquinone, vitamin C (L-Ascorbic acid) or derivatives thereof, and various plant extracts. there is By inhibiting the synthesis of melanin pigment, they can brighten the skin tone and realize skin whitening, as well as improve skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, or heredity. However, when applied to the skin, there is a problem in that the amount of use is limited due to safety problems such as irritation and redness, or a practical effect cannot be expected because the effect is insignificant.

Collagen is a major matrix protein produced by fibroblasts of the skin and is present in the extracellular matrix. Its important functions include mechanical robustness of the skin, resistance and cohesiveness of connective tissue, support of cell adhesion, cell division and differentiation. (in the growth of organisms or wound healing), etc. are known. Such collagen is reduced by age and photoaging caused by ultraviolet irradiation, and collagen reduction is promoted by the activity of collagenase enzyme that decomposes collagen. It is known that it is closely related to the formation of wrinkles in the skin.

In addition, collagen is a matrix protein that plays an important role in retaining moisture in the dermal layer, and these matrix proteins absorb moisture and increase the moisture retention capacity inside the structure they form so that the skin can maintain an appropriate moisture content. It is known to be involved in maintaining skin elasticity.

Currently, retinoids, adenosine, animal placenta-derived proteins, chlorella extracts, and the like are known as cosmetics for enhancing skin elasticity and improving wrinkles. Retinol, the most well-known, is a substance that promotes collagen synthesis, but it is unstable, and its usage is limited due to safety issues such as irritation and redness when applied to the skin. It is known to be difficult to predict.

The human body has a balance of antioxidants and antioxidants, but when these balances are lost due to various factors and tilted in the direction of promoting oxidation, oxidative stress is induced in the body, resulting in cell damage and cause pathological diseases. Reactive oxygen species (ROS), a direct cause of such oxidative stress, are chemically unstable and highly reactive, and can easily react with various biomaterials such as DNA, proteins, lipids, and carbohydrates, and can form polymers in vivo. It attacks cells and tissues to cause irreversible damage or to cause mutations, cytotoxicity, and cancer.

Meanwhile, macrophages in the human body respond to pathogens and induce inflammation such as tumor necrotic factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). factor, and synthesizes inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) to generate nitric oxide (NO) and prostaglandin (prostaglandin E2, PGE2). ) to create Physiologically, NO plays a variety of roles, including eliminating bacteria and tumors, regulating blood pressure, and mediating nerve transmission. However, when an inflammatory reaction occurs, the expression of iNOS in related cells increases and a large amount of NO is produced. Excessive production of NO causes tissue damage, genetic mutation, nerve damage, etc. promotes the inflammatory response.

During the inflammatory reaction, various inflammatory factors and various free radicals are generated. Normal free radicals are involved in maintaining cell homeostasis and affect cell differentiation, growth, survival, and aging. Among these free radicals, reactive oxygen species (ROS) are continuously generated in mitochondria in cells through oxidation and reduction processes of oxygen by respiration and immune responses. Harmful ROS are usually superoxide dismutase, catalase, glutathione peroxidase, glutamine reductase, vitamin C, vitamin E, uric acid, bilirubin and It is eliminated by the action of the same antioxidant system. However, when the balance between generation and elimination of free radicals is disrupted, oxidative stress occurs, resulting in various pathological changes such as inflammation, aging, or cancer. In fact, it has been confirmed that oxidative stress is increased in many clinical diseases, and various studies on new antioxidants are being conducted to reduce this oxidative stress.

Research on ingredients that protect the skin from environmental stimuli, stress, chemical procedures, etc. caused by various causes, and are ecologically friendly and harmless to the environment and humans is ongoing.

As described above, the cosmetic compositions described above have problems such as causing economic burden and pain to people, causing side effects, and having temporary effects or using only fragmentary pharmacological mechanisms, so that the effect is more excellent and lasting, The demand for compositions having no side effects is increasing.

Under this background, the present inventors have conducted research on materials with excellent skin whitening, skin elasticity enhancement, wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory effects, and thus a composition containing a mixed extract of rose, peony and iris as an active ingredient. It was found that the total amount of melanin was reduced, collagen synthesis was promoted, moisturizing, anti-inflammatory and antioxidant effects were exhibited, and cosmetic compositions, functional food compositions and quasi-drug compositions using the same were completed.

Republic of Korea Patent No. 10-0920896 Republic of Korea Patent No. 10-1638538

The main object of the present invention is to provide a cosmetic composition comprising a mixed extract of blue lotus and water lily as an active ingredient.

Another object of the present invention is to provide a functional food composition containing a mixed extract of blue lotus and water lily as an active ingredient.

Another object of the present invention is to provide a quasi-drug composition comprising a mixed extract of blue lotus and water lily as an active ingredient.

In order to achieve the above object, one aspect of the present invention provides a cosmetic composition comprising a mixed extract of blue lotus and water lily as an active ingredient.

The term "blue lotus (Nymphaea Caerulea)" used in the present invention refers to water lilies and lotuses that bloom in the water as fairies of water. In Egyptian mythology, it is recorded as a lotus that rose from the primordial water. In ancient Egypt, the sky-blue lotus was especially valued since ancient times. The petals spread like the sun's rays, bloom in the morning and close in the evening, and were considered more valuable as they grew in the Nile River, which gives life to land and living things. The petals and seeds of the blue lotus are known to have the effect of stabilizing the mind.

The term "Nymphaea Alba" used in the present invention refers to water lilies and lotuses that bloom in the water as fairies of water. It is so beautiful that it is called 'White Water Rose' and is widely loved. Also called 'Water lily with white flowers', it is known to have a noble and elegant beauty like a lily. It blooms well even in cold regions, and has the characteristic of blooming in the morning and curling up before sunset. Kite is also used as a very useful herbal medicine in oriental medicine, and most of the kite is used from fruit to leaf, flower, lotus rice, pistil of lotus, and root. The most used part is the fruit, which helps to energize, protects the five viscera, and eliminates thirst and diarrhea. In particular, it has the effect of stabilizing the mind, so it is a useful medicine for modern people with sensitive nerves.

In the present invention, "extract" refers to a liquid component obtained by immersing a desired material in various solvents and then extracting at room temperature, low temperature or elevated temperature for a certain period of time, obtained by removing the solvent from the liquid component It means the result of one solid content, etc. In addition, in addition to the above results, it can be comprehensively interpreted as including all dilutions of the results, concentrates thereof, adjusted products, and purified products thereof.

In the present invention, the extract means blue lotus, water lily extract. The blue lotus and water lily extracts can be extracted from various organs of natural, hybrid, and mutant plants, for example, roots, aerial parts, stems, leaves, flowers, fruit bodies, and fruit skins, as well as plant tissues. . The blue lotus and water lily extracts can be obtained by extraction with water or various organic solvents. At this time, the organic solvent used is not particularly limited thereto as long as an extract can be obtained, but specifically, it may be water, a polar solvent or a non-polar solvent, and more specifically, water, a lower alcohol having 1 to 6 carbon atoms ( methanol, ethanol, propanol, or butanol, etc.), and a mixed solvent thereof, and most specifically, methanol or a mixed solvent thereof. In addition, sugars may be included as solvents of the extract in the present invention. Specifically, sugars may be honey, royal jelly, sugar, fructose, sugar alcohols, fructooligosaccharide, etc., but are not limited thereto.

In addition, the method for obtaining the extract is not particularly limited as long as the blue lotus and water lily extracts can be obtained, but specifically, the blue lotus and water lily roots, stems, leaves, fruits, flowers, dried products thereof, Methods such as a cold needle extraction method in which a processed material is immersed in the solvent and extracted at room temperature, a heating extraction method, an ultrasonic extraction method in which ultrasonic waves are applied, and a reflux extraction method using a reflux condenser may be used.

The term "mixed extract" used in the present invention may be an extract obtained by mixing extracts of each component of blue lotus and water lily in an appropriate weight mixing ratio, and also, extract after mixing blue lotus and water lily in an appropriate weight mixing ratio. can It was confirmed that the mixed extract was more effective in skin whitening, skin elasticity improvement or wrinkle improvement, skin moisturizing, skin aging prevention, antioxidant, and skin inflammation improvement than each blue lotus and water lily single extract, and it was found that it had an excellent synergistic effect. there is.

The weight blending ratio of the blue lotus extract and the water lily extract is 1-25: weight ratio (w/w) of 1-25, specifically 1-15: weight ratio (w/w) of 1-15, more specifically 1-5 : It may have a weight ratio (w/w) of 1 to 5, and may have the same weight mixing ratio.

In the present invention, sugars may be further included in the mixed extract. Sugars include honey, royal jelly, sugar, fructose, sugar alcohols, fructooligosaccharide, etc., but are not limited thereto, and any material commonly used in the art may be used. More specifically, honey and royal jelly can be used as sugars.

The term "honey" used in the present invention means that bees collect from the nectar glands of flowers and store them as food. Honey is divided into native honey and beekeeping honey according to the type of bee, and is called acacia honey, bush clover honey, canola honey, chestnut honey, and buckwheat honey depending on the flower, and the color and taste vary depending on the type of flower. Honey is about 80% carbohydrate, 36-38% fructose, 34-36% glucose, and 2-3% sugar and dextrin. In addition, it contains 0.2~0.3% of protein, 0.05~0.2% of ash, vitamins B complex B1, B2, B6, pantothenic acid, formic acid, lactic acid, malic acid, pigment, rubber, wax, and enzymes. In Korea, honey is widely used for food and medicine, and is also used as a sweetener.

The term "royal jelly" used in the present invention is a 'gift from worker bees to the king' and refers to the queen bee's food. Royal jelly is known to have various efficacies as it contains a mysterious nutritional substance that contains proteins, fats, carbohydrates, and various trace elements, and a component called R that has not yet been identified. In addition, the ingredients of royal jelly itself are known to be effective for skin beauty as well as nutrition.

According to an embodiment of the present invention, when sugars such as honey and royal jelly are added to the mixed extract of blue lotus and water lily, collagen synthesis is enhanced and melanin production is increased compared to single extracts of blue lotus and water lily respectively. It was confirmed that the expression of HAS (Hyaluronan Synthases), a moisturizing factor, was increased, and the radical scavenging ability and NO production were suppressed.

Through this, when sugars such as honey and/or royal jelly are added to the mixed extract of blue lotus and water lily, synergistic action with the extract has a better effect on skin whitening, skin elasticity, skin moisturizing, anti-inflammatory and antioxidant effects. Could know.

In the present invention, the cosmetic composition may have effects such as skin whitening, skin elasticity enhancement and wrinkle improvement, skin moisturizing, antioxidant, anti-inflammatory, etc., but is not limited thereto.

"Skin whitening" in the present invention refers to not only brightening the skin tone by inhibiting the synthesis of melanin pigment, but also improving hyperpigmentation of the skin such as spots or freckles caused by ultraviolet rays, hormones or heredity.

According to a specific embodiment, the result of measuring the total amount of melanin after treating melanoma cells with a composition obtained by adding honey and/or royal jelly to the mixed extract of blue water lily and water lily or the mixed extract of blue water lily and water lily of the present invention. , It was confirmed that the mixed extract can be used for skin whitening because it shows a better melanin production inhibitory effect on melanoma cells when compared to the control or single extracts (Table 3).

Through this, when honey and / or royal jelly are added to the mixed extract of blue lotus and water lily or the mixed extract of blue lotus and water lily, a synergistic effect is generated compared to a single extract, and it can be seen that it has a better effect on skin whitening. there was.

In the present invention, "enhancement of skin elasticity" means to alleviate the degree of sagging or sagging of the skin, and elastic fibers composed of elastin exist together with collagen fibers called collagen, a state in which elastin and collagen are sufficiently present means that skin elasticity is maintained. In the present invention, "wrinkle improvement" refers to suppressing or inhibiting the formation of wrinkles on the skin, or alleviating already generated wrinkles.

According to a specific embodiment, HF (Human Fibroblast) cells treated with a composition obtained by adding honey or royal jelly to the mixed extract of blue lotus and water lily or the mixed extract of blue lotus and water lily of the present invention are cultured to synthesize procollagen As a result of the measurement, it was confirmed that the collagen synthesis effect was the best in the mixed extract (Table 2).

Through this, when honey and / or royal jelly are added to the mixed extract of blue lotus and water lily or the mixed extract of blue lotus and water lily, synergistic effect is generated compared to single extract, and it is known that it has a better effect on enhancing skin elasticity. could

In the present invention, "moisturizing the skin" means to increase the feeling of moisture in the skin and to maintain a moist state. The skin moisturizing effect can help improve wrinkles and increase elasticity of the skin.

According to a specific embodiment, keratinocytes (HaCaT, human keratinocyte) are cultured in a composition obtained by adding honey and/or royal jelly to the mixed extract of blue lotus and water lily or the mixed extract of blue lotus and water lily of the present invention, and HAS3 As a result of testing the moisturizing power by comparing the amount of mRNA, it was confirmed that the HAS3 mRNA expression increased when the mixed extract was treated compared to the control group and the single extract (Table 4).

Through these results, it was found that the mixed extract can exhibit a skin moisturizing effect.

Through this, when honey and / or royal jelly are added to the mixed extract of blue lotus and water lily or the mixed extract of blue lotus and water lily, a synergistic effect is generated compared to a single extract, and it can be seen that it has a better effect on skin moisturizing. there was.

In the present invention, "antioxidation" means an action of inhibiting oxidation, and although the human body has a balance of prooxidants and antioxidants, this balance is unbalanced by various factors. When it is achieved and tilted towards oxidation promotion, oxidative stress is induced, resulting in potential cell damage and pathological diseases. Reactive oxygen species (ROS), a direct cause of this oxidative stress, are unstable and highly reactive, easily reacting with various biomaterials, and attacking macromolecules in the body to cause irreversible damage to cells and tissues, mutations, It causes cytotoxicity and carcinogenesis. Reactive nitrogen species (RNS) such as NO, HNO ₂ , and ONOO ^- are produced in large quantities due to immune responses of macrophages, neutrophils, and other immune cells during inflammatory reactions, and ROS are also produced at this time. Active oxygen as described above oxidizes and destroys cells in the body, and thus is exposed to various diseases. Therefore, when the blue lotus and water lily extracts of the present invention are included in cosmetics, they can contribute to health promotion by achieving an antioxidant effect.

According to a specific embodiment, by analyzing the DPPH radical scavenging ability (Table 5) of the mixed extract of blue lotus and water lily of the present invention, it was confirmed that it has strong antioxidant activity. It was confirmed that the IC ₅₀ value of the mixed extract was reduced compared to that of the single extract. Through these results, it was found that the mixed extract can exhibit an antioxidant effect.

Through this, when honey and / or royal jelly were added to the mixed extract of blue lotus and water lily or the mixed extract of blue lotus and water lily, synergistic action was observed compared to single extract, and it was found to have a better antioxidant effect. .

"Anti-inflammatory" in the present invention means an action of suppressing inflammation, and the regulation of the inflammatory response is known to be very complex, which is known to enhance and reduce damage to the repair system in vivo. However, if the inflammatory response continues due to repeated tissue damage or regeneration, ROS and RNS are excessively produced in inflammation-related cells, resulting in permanent genetic modification. As such, ROS and RNS are deeply related to the inflammatory response that regulates the actions of various cells in vivo. During the inflammatory process, large amounts of the proinflammatory cytokines, nitric oxide (NO) and prostaglandin E2 (PGE2) are released into inducible nitric oxide synthase (iNOS) and cyclooxygenase enzymes. (cyclooxygenase-2, COX-2). Inflammation is a cause of various inflammatory diseases, and the composition containing the mixed extract of blue lotus and water lily of the present invention can have preventive and ameliorative effects on various inflammatory diseases through anti-inflammatory action.

According to a specific embodiment, as a result of analyzing the NO production inhibitory effect of the mixed extract of blue lotus and water lily of the present invention, a higher NO production inhibitory ability was confirmed when treated at the same concentration in the mixed extract compared to the single extract. It was confirmed that the mixed extract had a strong anti-inflammatory effect (Table 6).

Through this, when honey and / or royal jelly are added to the mixed extract of blue lotus and water lily or the mixed extract of blue lotus and water lily, synergistic effect is generated compared to a single extract, resulting in a better anti-inflammatory effect. It can be seen that there was.

The cosmetic composition according to the present invention is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleanser, bath additive, sunscreen cream, sun oil, suspension, Emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, may be prepared in a formulation selected from the group consisting of patches and sprays, but is limited thereto It is not.

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as typical ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, A thickener, a chelating agent, a colorant, a preservative, a flavoring agent, and the like may be suitably blended, but are not limited thereto.

Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation.

When the dosage form of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these may be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, and in particular, in the case of a spray, chloro propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan may be used. there is.

In the case where the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohols, suspending agents such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier components. can

The cosmetic composition of the present invention includes sugars of honey or royal jelly in addition to the mixed extract of blue lotus and water lily or the mixed extract of blue lotus and water lily, as well as auxiliary agents commonly used in cosmetic compositions, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic actives, preservatives, antioxidants, solvents, fragrances, fillers, maskers, pigments, odorants and dyes, and the like.

As another aspect for achieving the above object, the present invention provides a functional food composition containing a mixed extract of blue lotus and water lily as an active ingredient.

In the present invention, the functional food composition may further include sugars. Sugars include honey, royal jelly, sugar, fructose, sugar alcohols, fructooligosaccharide, etc., but are not limited thereto, and any material commonly used in the art may be used.

In the present invention, the functional food composition may have effects such as skin whitening, skin elasticity promotion and wrinkle improvement, skin moisturizing, antioxidant, anti-inflammatory, etc., but is not limited thereto.

The blue lotus flower, water lily extract, mixed extract, honey, royal jelly, skin whitening, skin elasticity promotion and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory are as described above. The functional food composition may be used in the form of health functional food, but is not limited thereto.

The functional food composition containing the mixed extract of blue lotus and water lily contained in the functional food composition of the present invention as an active ingredient may be included in the form of an extract thereof, a fraction thereof, or a processed product thereof. In addition, the composition may include a food additive that is acceptable in food science in addition to the active ingredient.

In the present invention, "food supplement additive" refers to a component that can be added to food supplementally, and can be appropriately selected and used by those skilled in the art as being added to prepare a health functional food of each formulation. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners , pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.

The functional food composition of the present invention may include health functional food. In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, "functionality" means obtaining useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions. The health functional food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation. In addition, the formulation of the health functional food may also be manufactured without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time using food as a raw material, and has excellent portability, so that the composition of the present invention Health functional food can be consumed as an adjuvant for enhancing skin whitening, skin elasticity enhancement or wrinkle improvement, or skin moisturizing, antioxidant, and anti-inflammatory effects.

There is no limit to the form that the health functional food of the present invention can take, and it can include all foods in a conventional sense, and can be used interchangeably with terms known in the art, such as functional foods. In addition, the health functional food of the present invention can be prepared by mixing suitable other auxiliary ingredients and known additives that can be included in food according to the selection of those skilled in the art. Examples of food that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and Vitamin complexes, etc., can be prepared by adding the compound represented by Formula 1 according to the present invention to juice, tea, jelly, juice, etc. prepared as a main component. It also includes food used as feed for animals.

As another aspect for achieving the above object, the present invention provides a quasi-drug composition comprising a mixed extract of blue lotus and water lily as an active ingredient.

In the present invention, the quasi-drug composition may contain chugo saccharides. Sugars include honey, royal jelly, sugar, fructose, sugar alcohols, fructooligosaccharide, etc., but are not limited thereto, and any material commonly used in the art may be used.

In the present invention, the quasi-drug composition may have effects such as skin whitening, skin elasticity enhancement and wrinkle improvement, skin moisturizing, antioxidant, anti-inflammatory, etc., but is not limited thereto.

The blue lotus flower, water lily extract, mixed extract, honey, royal jelly, skin whitening, skin elasticity promotion and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory are as described above.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent as needed in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, aromatics, preservatives, and the like. can include

Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , injectable esters, Witepsol, Macrogol, Tween 61, cacao butter, laurage, and the like.

In addition, when the composition containing the mixed extract of blue lotus and water lily of the present invention as an active ingredient is used as a quasi-drug, it may additionally contain one or more active ingredients exhibiting the same or similar functions. For example, known skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing ingredients may be included. When additional skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing ingredients are included, the skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing effects of the composition of the present invention can be further increased. When adding the above components, skin safety according to combined use, ease of formulation, and stability of active ingredients may be considered. The quasi-drug composition is a whitening component known in the art, a substance that inhibits tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, vitamin-C (L-Ascorbic acid); As skin elasticity, wrinkle improvement or moisturizing ingredients known in the art, retinoic acid, TGF, protein derived from animal placenta, betulinic acid and chlorella extract; And it may further include one or two or more components selected from the group consisting of derivatives thereof and various plant extracts. Additional components may be included in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety and ease of use.

The quasi-drug composition of the present invention may include, but is not limited to, a disinfectant cleanser, shower foam, ointment, wet tissue, coating agent, etc. It can be appropriately selected from the description of.

In addition, the quasi-drug composition containing the mixed extract of blue lotus and water lily of the present invention as an active ingredient is applied to the skin of an object, for skin whitening, skin elasticity promotion or wrinkle improvement, or skin moisturizing method can be used The subject includes, without limitation, mammals including mice, livestock, humans, and the like.

The cosmetic composition comprising the mixed extract of blue lotus and water lily of the present invention as an active ingredient has excellent skin whitening, elasticity enhancement or wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory effects. Specifically, the composition containing the mixed extract of the present invention exhibits the effect of reducing the total amount of melanin, has excellent collagen synthesis promoting effect and moisturizing effect, and is superior in NO production inhibitory effect and DPPH radical scavenging ability to skin whitening and elasticity enhancement compared to single extracts. It is highly applicable as a cosmetic composition for wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory use. In addition, the composition comprising the mixed extract of blue lotus and water lily of the present invention as an active ingredient can be used as a functional food composition and a quasi-drug composition.

Hereinafter, the present invention will be described in detail by means of the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

manufacturing example One. blue lotus , Preparation of water lily extract

After washing the blue lotus flower and water lily with water, the washed blue lotus flower and water lily were placed in an extraction container and extracted slowly at room temperature by adding an extraction solvent. The obtained extract was filtered to remove solids using filter paper or the like, and then the extract was concentrated under reduced pressure to prepare each extract.

manufacturing example 2. Preparation of Mixed Extracts

A mixed extract was prepared by injecting the same amount of each single extract of blue lotus and water lily prepared in Preparation Example 1. Mineral water or saccharide honey and/or royal jelly was added to each mixed extract. An example of the preparation ratio of the mixed extract is shown in Table 1.

ingredient Example One( % ) Example 2 ( % ) Example 3 ( % ) Example 4 ( % ) mineral water To 100 - - - blue blue lotus 34 34 34 34 training 34 34 34 34 honey To 100 - 16 royal jelly To 100 16

Based on each extract of Preparation Example 1, the content of Examples 1 to 4 was determined. In the case of Examples 1 to 4, the maximum content ratio was adjusted so that each extract was mixed in an equal ratio to produce a synergistic effect through cooperative action.

Experimental example 1. Collagen synthesis enhancement effect

For each single extract of blue lotus flower and water lily prepared in Preparation Example 1, honey, royal jelly, and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, the collagen synthesis enhancing effect was obtained using cells cultured as follows. measured.

Synthesis of procollagen was measured using a kit (procollagen type-1 C-peptide EIA kit, Takara, Japan). HF (Human Fibroblast) cells were put into a 24-well plate at a density of 5 × 10 ⁴ cells / ml, and no addition (control group), each single extract prepared in Preparation Example 1, honey, royal jelly and prepared in Preparation Example 2 0.01% by weight of the mixed extracts of Examples 1 to 4 were added and cultured for 48 hours in a 37°C, 5% CO ₂ incubator. Into an anti-PIP antibody-coated 96-well plate, 100 μl of antibody-POD conjugate sol'n was added, and each single sample prepared in Preparation Example 1 was added. 20 μl of the standard solution was added to the extract, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, and incubated at 37° C. for 3 hours after being shielded with aluminum foil.

After removing the culture medium, washing it four times with PBS, removing it, adding 100 μl of substrate solution, incubating at room temperature for 15 minutes, adding 100 μl of H ₂ SO ₄ , shaking lightly, and measuring absorbance at 450 nm. The experimental results are shown in Table 2. All results were expressed as mean±standard deviation (SD) through three or more independent experiments.

collagen expression Increase (%) (n=3) division Collagen synthesis increase rate (%) untreated 0 blue blue lotus 5.8 training 10.2 honey 7.05 royal jelly 11.73 Example 1 17.6 Example 2 17.9 Example 3 18.68 Example 4 21.3

As a result, as shown in Table 2, when treating the mixed extract of Example 1 prepared in Preparation Example 2, it was confirmed that there was an effect of increasing collagen synthesis superior to each single extract prepared in Preparation Example 1, and Preparation Example 2 It was confirmed that the mixed extracts of Examples 2 to 4 prepared in had a more excellent collagen synthesis increasing effect than the mixed extract of Example 1 by honey and/or royal jelly.

Experimental example 2. Melanin production inhibitory effect

In order to confirm the whitening effect of each single extract of blue lotus flower and water lily prepared in Preparation Example 1, honey, royal jelly, and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, the cells cultured as follows The melanin inhibitory effect was measured using.

Specifically, the whitening efficacy of the sample was evaluated by measuring the degree of decrease in the amount of melanin pigment present in melanocytes. The sample was diluted to 0.01% by weight, treated with B16 melanocytes, cultured for 48 hours, and whitening efficacy was evaluated by quantifying melanin. The melanin content was calculated based on the total protein content and then obtained as a percentage based on the DMSO control group. Arbutin 100 ppm (Lim YJ et al., 2009, Arch Pharm Res.) was used as a positive control.

The results are shown in Table 3 below. All results were expressed as mean±standard deviation (SD) through three or more independent experiments.

Amount of melanin produced (%) (n=3) sample Melanin production amount (%) untreated 100 Arbutin 55 blue lotus 94 training 85 honey 120 royal jelly 146 Example 1 78 Example 2 72 Example 3 75 Example 4 68

As a result, as shown in Table 3, when treating the mixed extract of Example 1 prepared in Preparation Example 2, it was confirmed that there is a superior melanin production inhibitory effect than each single extract prepared in Preparation Example 1, Preparation Example 2 It was confirmed that the mixed extracts of Examples 2 to 4 prepared in had a more excellent melanin production inhibitory effect than the mixed extract of Example 1 by honey and/or royal jelly.

Experimental example 3. Moisturizing factor synthetic factor enhancing effect

In order to confirm the moisturizing factor synthesis factor enhancing effect for each single extract of blue lotus flower and water lily prepared in Preparation Example 1, honey, royal jelly, and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, as follows. The skin moisturizing effect was measured using the cultured cells.

Specifically, 10% FBS (Gibco), streptomycin 100 μg/ml, penicillin 100 μg/ml, amphotericin B 0.25 μg/ml under conditions of 37° C. and 5% CO ₂ . Keratinocytes (HaCaT, human keratinocyte) were proliferated using DMEM medium supplemented with ㎖.

Specifically, 10% FBS (Gibco), streptomycin 100 μg/ml, penicillin 100 μg/ml, amphotericin B 0.25 μg/ml under conditions of 37° C. and 5% CO ₂ . Keratinocytes (HaCaT, human keratinocyte) were proliferated using DMEM medium supplemented with ㎖.

The formed keratinocytes (HaCaT, human keratinocyte) were dispensed with 3 ml of medium at a density of 5 X 10 ⁵ cells/well in 6 wells, and cultured for 24 hours in a 37°C, 5% CO ₂ incubator. The cultured medium was washed with PBS to remove FBS. In the medium from which FBS was removed, untreated, each single extract of rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly, and Examples 1, 2, 3, and 4 prepared in Preparation Example 2 were added at 0.01% by weight. After treatment with 3 ml each, they were cultured again, and the amount of HAS3 (Hyaluronan Synthases 3) mRNA was compared. RNA was used with an RNA extraction kit (RNeasy Mini Kit, Qiagen, USA), cDNA was synthesized using SuperScript II reverse transcriptase (Invitrogen, USA), and Platinum PCR supermix (Invitrogen, USA)) and PCR was performed using the prepared primers. For cDNA synthesis, 1 μg of total RNA was heated at 50° C. for 1 hour and 85° C. for 5 minutes, and then the reaction was stopped. PCR amplification was denatured at 94°C for 50 seconds, annealed at 60°C for 50 seconds, and extended at 72°C for 50 seconds. This was repeated 26 cycles, and the final extension was performed at 72° C. for 5 minutes. The amplified product was electrophoresed on a 2% agarose gel to detect gene expression using the Fusion Fx5 detection system (VilberLourmat, Germany). confirmed by

The results are shown in Table 4 below. All results were expressed as mean±standard deviation (SD) through three or more independent experiments.

Increased HAS expression (%) (n=3) division HAS mRNA Expression ( % ) untreated 100 blue lotus 106 training 98 honey 112 royal jelly 115 Example 1 109 Example 2 124 Example 3 126 Example 4 132

As a result, as shown in Table 4, when treating the mixed extract of Example 1 prepared in Preparation Example 2, it was confirmed that HAS expression increased compared to each single extract prepared in Preparation Example 1, and prepared in Preparation Example 2. It was confirmed that the HAS expression of the mixed extracts of Examples 2 to 4 increased more than the mixed extract of Example 1 by honey and/or royal jelly.

Experimental example 4. Antioxidant effect

In order to confirm the antioxidant effect of each single extract of blue lotus flower and water lily prepared in Preparation Example 1, honey, royal jelly and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, the cells cultured as follows The skin antioxidant effect was measured using this method.

Specifically, the radical scavenging ability of the sample was evaluated using the change of DPPH from purple to yellow when radicals present in DPPH were scavenged. Vit C was used as a control. The sample was used after being dissolved in methanol. The experimental method was to mix 0.15 mM DPPH solution and 100 μl of the sample, react at room temperature for 30 minutes, and measure the absorbance (A540) at 540 nm for the color change of DPPH. The IC ₅₀ value was obtained through the A540 value, and the radical scavenging ability was compared and evaluated.

The results are shown in Table 5 below. All results were expressed as mean±standard deviation (SD) through three or more independent experiments.

radical erasing ability (IC ₅₀ ) (n=3) division IC ₅₀ Vitamin C 0.0005 blue lotus 0.267 training 0.283 honey 12.04 royal jelly 6.049 Example 1 0.21 Example 2 0.241 Example 3 0.208 Example 4 0.19

As a result, as shown in Table 5, when treating the mixed extract of Example 1 prepared in Preparation Example 2, it was confirmed that there was a superior antioxidant effect than each single extract prepared in Preparation Example 1, prepared in Preparation Example 2 It was confirmed that the mixed extracts of Examples 2 to 4 had a more excellent antioxidant effect than the mixed extract of Example 1 by honey and/or royal jelly.

Experimental example 5. Anti-inflammatory effect

In order to confirm the anti-inflammatory effect of each single extract of blue lotus flower and water lily prepared in Preparation Example 1, honey, royal jelly, and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, the cells cultured as follows The skin anti-inflammatory effect was measured using

Specifically, the NO production inhibitory effect of the extracts was measured using Raw 264.7 cells. As a positive control, 20 mg/ml L-NMMA1 (NOS inhibitor) was used, and the sample was diluted to a concentration of 0.01% by weight, treated 30 minutes before treatment, treated with 1 μg/ml LPS, incubated for 24 hours, and NO quantification kit The NO production inhibitory ability was evaluated as follows.

The results are shown in Table 6 below. All results were expressed as mean±standard deviation (SD) through three or more independent experiments.

NO production inhibitory effect (%) (n=3) division NO production inhibitory effect ( % ) positive control 42 blue lotus 19.4 training 18.4 honey 13.1 royal jelly 15.5 Example 1 25.7 Example 2 27 Example 3 28.3 Example 4 31

As a result, as shown in Table 6, when treating the mixed extract of Example 1 prepared in Preparation Example 2, it was confirmed that the NO production inhibition ability was higher than that of each single extract prepared in Preparation Example 1, and the extract prepared in Preparation Example 2 The mixed extracts of Examples 2 to 4 were confirmed to have a higher NO production inhibitory ability than the mixed extract of Example 1 by honey and/or royal jelly.

That is, it can be seen that the mixed extract (Example 1) according to the present invention has a synergistic effect superior to each of the blue lotus and water lily single extracts, and when honey and / or royal jelly are added through Examples 2 to 4 , it can be seen that the synergistic action of the mixed extract is higher.

Through these results, it can be seen that the mixed extract is very effective in skin whitening, skin elasticity enhancement or wrinkle improvement, skin moisturizing, skin aging prevention, antioxidant, and skin inflammation improvement effects.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (13)

A cosmetic composition comprising a mixed extract of blue lotus and water lily as an active ingredient.
According to claim 1,
A cosmetic composition further comprising honey, royal jelly, or both.
According to claim 1 or 2,
The cosmetic composition is for skin whitening, the cosmetic composition.
According to claim 1 or 2,
The cosmetic composition is for enhancing skin elasticity or improving wrinkles, a cosmetic composition.
According to claim 1 or 2,
The cosmetic composition is for moisturizing the skin, the cosmetic composition.
According to claim 1 or 2,
The cosmetic composition is for antioxidant, cosmetic composition.
According to claim 1 or 2,
The cosmetic composition is for anti-inflammatory, cosmetic composition.
A functional food composition comprising a mixed extract of blue lotus and water lily as an active ingredient.
According to claim 8,
A functional food composition further comprising honey, royal jelly, or both.
The method of claim 8 or 9,
The functional food composition is for skin whitening, skin elasticity enhancement or wrinkle improvement, skin moisturizing, antioxidant or anti-inflammatory, functional food composition.
A quasi-drug composition comprising a mixed extract of blue lotus and water lily as an active ingredient.
According to claim 11,
A quasi-drug composition further comprising honey, royal jelly, or both.
According to claim 11 or 12,
The quasi-drug composition is a quasi-drug composition for skin whitening, skin elasticity promotion or wrinkle improvement, skin moisturizing, antioxidant or anti-inflammatory use.