KR102076746B1 - Mixed cosmetic composition comprising flower - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a mixture comprising safflower, cockscomb, peach blossoms and cornflower flowers as an active ingredient, and relates to a cosmetic composition having an excellent skin-improving effect.

Description

Mixed cosmetic composition containing a flower {MIXED COSMETIC COMPOSITION COMPRISING FLOWER}

The present invention relates to a cosmetic composition having a skin improving effect comprising a mixture comprising safflower, cockscomb, peach blossoms and cornflower flowers as an active ingredient.

In the aging society, interest in health has recently increased, and among them, interest in functional substances using natural foods has increased.

Neutraseutical, which is known to cure or prevent diseases caused by various active ingredients, is an issue. Representative ingredients of Nutraceutical include probiotics, peptides and proteins, phytochemicals and plant extracts, prebiotics, dietary fiber and special carbohydrates, amino acids, omega-3 fatty acids and complex linoleic acids and minerals in natural foods. , Vitamins, carotenoids and antioxidants .

One of these natural foods is an edible flower, which is an edible flower, and is generally used to enhance the color, aroma, taste, and appetite of a food or as a main ingredient of cooking. Edible flowers are rich in nutrients such as antioxidants, minerals and vitamins, and are used as very good food ingredients. They are also widely used as medicines because they do not use pesticides or chemical fertilizers.

Since ancient times in Korea, tea is made from flowers bloomed every season and placed next to them.In China, it is believed that flowers have an excellent effect to beautify women's faces and prevent them from aging. It has also been widely used as a folk remedy.

Accordingly, the present invention is to provide a cosmetic composition having a skin improvement effect using natural foods having fewer side effects on the human body and exhibiting various effective effects.

It is an object of the present invention to provide a cosmetic composition exhibiting a skin-improving effect by using safflower, cockscomb, peach blossoms, and cornflower flowers among natural foods having fewer side effects on the human body and exhibiting various effective effects.

In order to solve the above problems, the present invention provides a cosmetic composition for whitening and wrinkle improvement comprising a mixture containing safflower, cockscomb, peach blossoms and cornflower flowers as an active ingredient.

According to one embodiment, the mixture may be prepared by mixing safflower extract, cockscomb extract, peach blossom extract and cornflower extract.

According to another embodiment, the cosmetic composition may act as an inhibitor of melanin production.

According to another embodiment, the cosmetic composition may act as a biosynthesis promoter of collagen.

According to another embodiment, the cosmetic composition may act as a synthesis inhibitor of nitric oxide (Nitric Oxide, NO).

According to another embodiment, the cosmetic composition may act as an active oxygen inhibitor.

According to another embodiment, the cosmetic composition is a lotion, nutrient lotion, nourishing cream, massage cream, essence, pack, emulsified foundation, solid foundation, sunscreen cream, bath cleaner, shampoo, soap, mist or aerosol It may be a formulation.

As the cosmetic composition of the present invention includes a mixture containing safflower, cockscomb, peach blossoms and cornflower flowers as an active ingredient, free radicals are eliminated, melanin production is inhibited, collagen biosynthesis is promoted, and whitening and wrinkles, etc. It may have a skin improving effect.

In addition, the cosmetic composition of the present invention may exhibit an antioxidant effect by inhibiting free radicals, the synthesis of nitric oxide is inhibited, it may exhibit an anti-inflammatory effect.

1 is a graph showing the active oxygen inhibitory effect of the cosmetic composition according to the present invention.
Figure 2 is a graph showing the tyrosinase activity inhibitory effect of the cosmetic composition according to the present invention.
3 is a graph showing the melanin production inhibitory effect of the cosmetic composition according to the present invention.
Figure 4 is a graph showing the collagen biosynthesis promoting effect of the cosmetic composition according to the present invention.
5 is a graph showing the inhibitory effect of nitric oxide synthesis of the cosmetic composition according to the present invention.

Hereinafter, the present invention will be described in detail.

The present invention relates to a cosmetic composition for whitening and improving wrinkles comprising a mixture comprising safflower, cockscomb, peach blossoms and cornflower flowers as an active ingredient.

The mixture may include safflower extract, cockscomb extract, peach blossom extract and cornflower extract.

The mixture may include a mixed extract prepared by mixing the safflower, the cockscomb, the peach blossom, and the cornflower flower.

The cosmetic composition may include the mixture, thereby acting as a melanogenesis inhibitor or collagen biosynthesis promoter, and thus the cosmetic composition may exhibit a whitening and wrinkle improvement effect.

In addition, the cosmetic composition may act as an inhibitor of nitric oxide synthesis by including the mixture, whereby the cosmetic composition may exhibit an anti-inflammatory effect.

The cosmetic composition may be provided in the form of lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, emulsified foundation, solid foundation, sunscreen cream, bath cleaner, shampoo, soap, mist or aerosol.

In addition, in the cosmetic composition of each formulation, in addition to the mixture containing the safflower extract, cockscomb extract, peach blossom extract and cornflower flower extract, other components may be arbitrarily selected and blended according to the formulation or purpose of use of other cosmetics. For example, a coloring agent (color), a flavoring agent (flavor), a suspending agent, an emulsifier, a dissolution aid, a stabilizer, an isotonic agent, a pH adjuster, a viscosity adjuster, a solvent, and the like can be included.

(1) safflower

The safflower included in the cosmetic composition according to the present invention promotes blood circulation to the flowers of safflower which are annual herb belonging to the Asteraceae, in particular, vitamin E contained in the seed of safflower can prevent aging in blood vessels.

The safflower ( Carthamus tinctorius L.), cold or menopausal disorders, pain economy (medication to loose blood), women's diseases, pain, abdominal pain, coronary artery relaxation, cardiac ischemia improvement, immunoregulation, anticoagulant, anti-aging, anti-hypoxia, It can have various effects on anti-fatigue, anti-inflammatory, anti-fibrosis and the like.

The safflower contains quinochalcones, flavonoids and components such as glycosides, alkaloids, polyacetyllins, glycosides and the like.

(2) cockscomb

The cockscomb ( Celosia cristata L.) included in the cosmetic composition according to the present invention is a perennial herb with the dicotyledonous plant central amaranth, the stem grows straight and has a height of 0.4-1.0m, hairless and usually reddish. The leaves of the cockscomb are displaced and have a long petiole in ovate or egg lanceolate. The flowers of the cockscomb bloom in red, yellow, white, etc. in July-August, and the flowers of the cockscomb can be cleared blood and provide the effect of hemostasis, called corolla. The cockscomb bleeding caused by hemorrhoids, bleeding from the urine, urine, urine, coughing, blood comes out, the abdominal pain and diarrhea symptom, redness, ecchymosis, skin eczema, it can provide a therapeutic effect.

(3) peach blossom

The painting ( Prunus persica Flos) included in the cosmetic composition according to the present invention is called a radiant flower, and the peach tree is also called a radiant tree. Blooming white peach of peach is called 'Cheongdobaekdo', and this peach flower contains ingredients such as kaempferol derived from flavonoids and albamyricetin, a glycoside of kaempferol, and kaempferol can provide lipid peroxidation and cholesterol suppression effects. . The taste of the peach blossom is bitter and has a flat nature, and can act on the stomach, liver and nerves to show efficacy.

 (4) cornflower

The cornflower flower included in the cosmetic composition according to the present invention, the flower extract of the cornflower (Centorrea) Centaurea cyanus L. is a diuretic and diuretic, has bactericidal activity, purifies the mind and body, It can relieve stress.

In addition, the cornflower flower extract has an anti-inflammatory action and soothing action may help calm the red skin.

The cornflower flowers have a calming effect, but when the eyes are tired or inflamed, the leaching solution of the leaves can also be used as eye drops, diuretic, antimicrobial and bronchitis can also help.

The present invention relates to a cosmetic composition for whitening and wrinkle improvement comprising a mixture comprising safflower, cockscomb, peach blossoms and cornflower flowers as an active ingredient, the mixture is safflower extract, cockscomb extract, peach blossom extract and cornflower flowers It may include an extract. Alternatively, the mixture may include a mixed extract prepared by mixing the safflower, cockscomb, peach blossoms, and cornflower flowers, followed by extraction.

The extraction solvent of the extracts may be at least one selected from the group consisting of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol and butylene glycol, preferably water And it may be at least one selected from the group consisting of anhydrous or hydrous alcohol having 1 to 4 carbon atoms. The anhydrous or hydrous alcohol having 1 to 4 carbon atoms is methanol, ethanol, n-propanol, iso-propanol, n-butanol and tert-butanol. In addition, most preferably, the hydrous ethanol of the extraction solvent may be used, the amount of water contained is preferably 30 to 90% by weight, most preferably 70% by weight.

The extracts may be prepared by the following extraction method. For example, the safflower extract, by adding 1 to 50 times the volume of the extraction solvent to the dry weight of the safflower, and immersed for 3 to 20 days at 4 to 30 ℃ to extract the active ingredient, the extraction solvent is concentrated in a reduced pressure concentrator Can be obtained. In addition, in order to prevent the solvent from evaporating, an extract having a cooling condenser is heated and extracted for 1 to 38 hours at 30 to 100 ° C. or deposited for 1 to 10 days at 5 to 30 ° C. to extract the active ingredient, and It can be obtained by concentrating with a vacuum concentrator. In the heat extraction method, the extraction temperature is preferably 50 to 100 ° C. In addition, it may be prepared using an ultrasonic extraction method in addition to the above production method.

The cockscomb extract, peach blossom extract and cornflower flower extract can also be prepared by the same known extraction method as above, in the case of a mixed extract extracted after mixing safflower, cockscomb, peach blossom and cornflower flowers Edo may be prepared by a known extraction method in the same manner as the above method.

The safflower extract, cockscomb extract, peach blossom extract. Cornflower flower extract and the mixed extract may be a concentrated solution concentrated under reduced pressure, powder freeze-dried the concentrate or a powder dried by spray drying the concentrate.

The mixture is a safflower extract, cockscomb extract, peach blossom extract and cornflower flower extract in a weight ratio of 1: 0.1 to 10: 0.1 to 10: 0.1 to 10, preferably 1: 0.1 to 5: 0.1 to 5: 0.1 to 5, and most preferably 2: may be mixed in a weight ratio of 2: 1: 1: 1.

The mixture may be included in an amount of 0.1 to 30% by weight based on the total weight of the cosmetic composition.

The mixture of the extracts in the above weight ratio may act as a melanin inhibitor or collagen biosynthesis promoter, and thus, the cosmetic composition including the mixture may exhibit a whitening and wrinkle improvement effect. In addition, the mixture may act as a nitric oxide synthesis inhibitor, and accordingly, the cosmetic composition including the mixture may exhibit an anti-inflammatory effect.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples, Examples, Comparative Examples and Experimental Examples. However, the following Preparation Examples, Examples, Comparative Examples and Experimental Examples illustrated in the present invention are for illustrating the present invention, and the present invention is not limited to the following Preparation Examples, Examples, Comparative Examples, and Experimental Examples.

Manufacturing Example 1.

10 L of hydrated ethanol containing 70% by weight of water was added to 1 kg of dried safflower, and then sufficiently aged at room temperature for 48 hours, and the filtrate was filtered through a filter paper (5C, 185 mm, commercially available from Toyoroshi Kaisha, Ltd., Japan). Concentrated to remove water, and then fully aged at room temperature for 24 hours to obtain a safflower extract.

Preparation Examples 2 to 4.

Except for using the cockscomb, peach blossoms and cornflower flowers instead of the safflower of Preparation Example 1 was carried out in the same manner as Preparation Example 1 cockscomb extract (Preparation Example 2), peach blossom extract (Preparation Example 3) and cornflower A flower extract (Production Example 4) was obtained.

Example 1. Mixture Preparation

Safflower extract, cockscomb extract, peach blossom extract and cornflower extract prepared in Preparation Examples 1 to 4 were mixed in a weight ratio of 2: 1: 1: 1 to prepare a mixture.

With respect to Preparation Examples 1 to 4 and Example 1, active oxygen inhibitory effect (Experimental Example 1), melanin production inhibitory effect (Experimental Examples 2, 3), collagen biosynthesis promoting effect (Experimental Example 4) and nitric oxide synthesis inhibitory effect (Experimental example 5) was measured.

Experimental Example 1. Measurement of electron donating ability

Referring to Figure 1, the active oxygen inhibitory effect of the cosmetic composition according to the present invention can be confirmed.

A reagent that measures the reducing power of antioxidants is the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. DPPH exists as a radical of a stabilized structure of the nitrogen center in the compound. The maximum absorption at 517 nm is shown and when reduced, there is no absorption at 517 nm (DPPH scavenging). DPPH (D9132, SIGMA) was dissolved in a 6: 4 (v / v) mixed solvent of methanol and distilled water, and the DPPH solution was adjusted to have an absorbance of about 0.6 at 513 nm. 0.1 ml of the DPPH solution was added to 0.2 ml of the extract diluted by concentration, and mixed. After reacting for about 20 minutes at room temperature, the absorbance was measured at 513 nm using an absorption spectrophotometer (UVmax, Molecular Device, USA). The DPPH erasure rate may be calculated through Equation 1 below.

[Equation 1]

Experimental Example 2. Inhibition of Tyrosinase Activity

Referring to Figure 2, it can be confirmed the tyrosinase inhibitory effect of the cosmetic composition according to the present invention.

For the experiment, tyrosinase enzyme solution was prepared by dissolving mushroom tyrosinase (mushroom tyrosinase, T-3824, 1530U / mg, Sigma) in phosphate buffer (pH 6.5) to 1000 U / ml, and the substrate solution L-tyrosine (L-tyrosine, 45160-0410, Junsei chemical co. Ltd) was prepared by dissolving in phosphate buffer (pH 6.5) to 1.5 mM.

Extracts diluted by concentration were prepared in the buffer, 170 μl of extract and 10 μl of tyrosinase enzyme solution were added and reacted at 37 ° C. for 10 minutes. 20 μl of the prepared substrate solution was added thereto, followed by reaction for 10 minutes at 37 ° C., and then left for 5 minutes in ice, followed by absorbance at wavelength 490 nm using an absorption spectrophotometer (UVmax, Molecular Device, USA). Measured. The absorbance measured above was substituted into Equation 2 below to calculate the tyrosinase activity inhibition rate.

The blank test solution was prepared by adding the same amount of phosphate buffer (pH 6.5) instead of the sample solution, and the calibration solution was prepared by adding the same amount of phosphate buffer (pH 6.5) instead of the substrate solution.

[Equation 2]

A: absorbance after the liquid reaction

B: absorbance after reaction of blank test solution

A ': calibration liquid

B ': Correction amount of blank

Experimental Example 3 Inhibition of Melanin Production

Referring to Figure 3, it can confirm the melanin production inhibitory effect of the cosmetic composition according to the present invention.

B16F10 melanoma cells derived from mice were added to a 6-well tissue culture dish in a 2MEM10 ⁴ cell / well concentration in DMEM medium to which 10% FBS (GIBCO, USA) was added, 5% CO2, 37 ° C. Incubated for 24 hours under the conditions.

After incubation, the medium was removed and replaced with DMEM medium containing 10% FBS, 2 uM α-MSH (Sigma., USA, Melanocyte stimulating Hormone) and 2 mM theophylline (Sigma., USA, theophylline), and diluted by concentration. After the addition of the extract, the cells were cultured under 5% CO 2, 37 ° C. until the cells were at least about 80% at the bottom of the well.

After incubation, the medium was removed, washed with PBS (Sigma. USA, Phosphate buffered Saline) and treated with trypsin to recover the cells. The recovered cells were centrifuged at 5000 rpm for 10 minutes and the supernatant was removed. Cells from which the supernatant was removed were dried for 24 hours at 60 ° C. incubator and lysed melanin by adding 1N NaOH. The absorbed melanin was measured at an absorbance spectrophotometer (Uvmax, Molecular Device, USA) at a wavelength of 490 nm. Melanin production inhibition rate can be calculated through the following equation (3).

[Equation 3]

A: absorbance after the liquid reaction

B: absorbance after reaction of blank test solution

A ': calibration liquid

B ': Correction amount of blank

Experimental Example 4. Biosynthesis of Collagen

Figure 4 is a graph showing the collagen biosynthesis promoting effect of the cosmetic composition according to the present invention.

Wrinkles in the skin are a common phenomenon as they age. Collagen is known to show a decrease in production with increasing age and to increase the decomposition to induce depression of the dermal dermal layer to produce wrinkles of the skin. Type I collagen production was measured using Takara's kit. This kit is a method to directly analyze collagen content by measuring procollagen type Icarboxy-terminal peptide (PIP) using polyclnal antibody.

In order to confirm the anti-wrinkle effect according to the present invention, the amount of type I procollagen biosynthesis of fibroblasts was measured using a procollagen type I Cpeptide ELISA kit (TaKaRa, MK101).

Fibroblasts were grown after inoculation on a 48 well plate at a concentration of 5 × 10 ⁴ cells / mL. The medium used was DMEM medium containing 10% FBS. After 24 hours after inoculation, the extract was added to the medium by concentration, and cultured in a 37 ° C., 5% CO 2 incubator for 24 hours. Procollagen type I C-peptide was measured using the supernatant obtained by transferring the medium to the tube and centrifugation.

Experimental Example 5. Inhibition of Nitric Oxide Synthesis

5 is a graph showing the inhibitory effect of nitric oxide synthesis of the cosmetic composition according to the present invention.

Nitric oxide (NO), one of the active nitrogen species (RNS) and known as an important factor in the inflammatory response, acts as a physiological phenomenon that regulates blood pressure and neurotransmitters, plays a pivotal role in the immune response. It is also expressed in cells such as chonodrocytes and synoviocytes. In addition, it has various physiological functions such as body defense function, signaling function, neurotoxicity, and vasodilation. In general, the formation of NO plays an important role in killing bacteria or eliminating tumors. Has been reported to cause tissue destruction and immune system abnormalities.

Various mediators are involved in the expression mechanism of biological inflammation, and the causes of the disease also vary. Treatment of LPS (lipopolysachride), known as endotoxin in macrophages, increases NO biosynthesis and induces inflammatory responses by iNOS (inducible NOS) expression. NO is a pathological secondary signaling substance secreted by many cells and regulates COX activity and synergistically acts on the inflammatory response.

After artificially treating LPS (lipopolysachride), an inflammatory inducer, Raw 264.7 cells were evaluated for their inhibitory effects.

Raw 264.7 cells were dispensed into a 96 well plate and incubated for 24 hours under conditions of 5% CO2 and 37 ℃. After overnight treatment, LPS and extracts were incubated for 24 hours, followed by centrifugation of the culture supernatant, and only the supernatant was collected and reacted with Griess reagent to measure absorbance at 570 nm.

Claims (13)

Contains a mixture containing safflower, cockscomb, peach blossoms and cornflowers as an active ingredient,
The mixture, safflower extract, cockscomb extract, peach blossom extract and cornflower extract,
The mixture, the safflower extract, the cockscomb extract, the peach blossom extract and the cornflower flower extract is mixed in a weight ratio of 1: 0.1 to 10: 0.1 to 10: 0.1 to 10 for whitening and wrinkle improvement Cosmetic composition.
delete delete delete The method according to claim 1,
The extraction solvent of the safflower extract, the cockscomb extract, the peach blossom extract and the cornflower flower extract is water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol and butylene glycol Cosmetic composition for improving whitening and wrinkles, characterized in that at least one selected from the group consisting of.
delete The method according to claim 5,
The extraction solvent is a cosmetic composition for improving whitening and wrinkles, characterized in that at least one selected from the group consisting of water and anhydrous or hydrous alcohol having 1 to 4 carbon atoms.
The method according to claim 1,
The mixture is a cosmetic composition for improving whitening and wrinkles, characterized in that contained in 0.1 to 30% by weight relative to the total weight of the cosmetic composition.
The method according to claim 1,
The mixture is a cosmetic composition for improving whitening and wrinkles, characterized in that acts as an inhibitor of the production of melanin.
The method according to claim 1,
The mixture is a cosmetic composition for improving whitening and wrinkles, characterized in that acts as a biosynthesis promoter of collagen.
The method according to claim 1,
The mixture is a cosmetic composition for improving whitening and wrinkles, characterized in that acts as a synthesis inhibitor of nitric oxide (Nitric Oxide, NO).
The method according to claim 1,
The mixture is a cosmetic composition for improving whitening and wrinkles, characterized in that acts as an active oxygen inhibitor.
The method according to claim 1,
The cosmetic composition is a skin lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, emulsified foundation, solid foundation, sunscreen cream, bath cleaner, shampoo, soap, mist or aerosol formulation, characterized in that Cosmetic composition for improving wrinkles.

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