KR102429214B1 - A composition for stimulating growth of hairs contaning natural complex extracts - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth, and includes rye extract, flaxseed extract, wheat germ extract, and borage extract as active ingredients.

Description

Composition for preventing hair loss and promoting hair growth containing a natural complex extract

The present invention relates to a composition capable of promoting hair growth while minimizing hair damage and irritation.

Animals have a molting period in which the entire hair is replaced according to the season, but in humans, a part of the total hair is continuously replaced every day, so that a similar number is always maintained.

The hair cycle can be divided into three cycles: growth phase, regression phase, and resting phase. Among them, the anagen phase is a period in which active cell division occurs in the dermal papilla to promote the growth of new hair. can

The hair growth cycle is about 3 to 5 years for men and 4 to 6 years for women, and about 80 to 85% of the hair in total corresponds to the growth phase.

The catagen phase is a period of gradual cessation of cell division and lasts about 3 to 4 weeks. Lastly, the talogen is a period in which the dermal papilla is atrophied and the hair is separated from the capillaries and is simply embedded in the scalp, for about 3 months.

Although human hair has nothing to do with life, it has a decisive effect on the impression and appearance of a person, so socially much attention is focused on it, and unlike the conventional hair loss was recognized as a symptom, the recognition as a disease is increasing in recent years.

In particular, with the recent acceleration of advancement and industrialization, the number of hair loss populations is rapidly increasing due to excessive stress and socio-cultural factors of lifestyle.

In addition, in recent years, hair loss not only in middle-aged men but also in the elderly is increasing, so research is being actively conducted to analyze and solve various causes of hair loss.

On the other hand, in the cosmetic industry, a number of products using natural products are being developed to reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic raw material is increasing.

Accordingly, the demand for cosmetics using natural ingredients with high consumer response is gradually increasing, and various studies on the development of multi-functional cosmetics having excellent biosafety and skin improvement effects are being actively conducted.

The present invention is to solve the problems of the prior art described above, and an object of the present invention is to provide a composition containing natural raw materials as an active ingredient, which is beneficial to skin health, as well as excellent in preventing hair loss and promoting hair growth.

According to one aspect of the present invention, there is provided a pharmaceutical composition for preventing hair loss and promoting hair growth comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients.

In one embodiment, the pharmaceutical composition may further include a pine bark extract or saw palmetto extract.

In one embodiment, the pharmaceutical composition may further include a Moringa extract.

In one embodiment, the pharmaceutical composition may further include biotin.

In one embodiment, the pharmaceutical composition may further include anti-inflammatory, antibacterial activity and cell proliferation.

According to another aspect of the present invention, there is provided a food composition for preventing hair loss and promoting hair growth comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients.

In one embodiment, the food composition may further include a pine bark extract or saw palmetto extract.

According to another aspect of the present invention, there is provided a cosmetic composition for preventing hair loss and promoting hair growth comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients.

In one embodiment, the cosmetic composition is one selected from the group consisting of hair toner, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair soap, hair shampoo, hair conditioner, hair pack, and hair treatment. It can be formulated as above.

In the composition according to one aspect of the invention, the active ingredients contained in the natural complex extract interact to maximize the effect of preventing hair loss and promoting hair growth while being beneficial to the user's skin health.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

The terms used in this specification have been selected as currently widely used general terms as possible while considering the functions in the present invention, which may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Numerical ranges are inclusive of the values defined in that range. Every maximum numerical limitation given throughout this specification includes all lower numerical limitations as if the lower numerical limitation were expressly written. Every minimum numerical limitation given throughout this specification includes all higher numerical limitations as if the higher numerical limitation were expressly written. All numerical limitations given throughout this specification will include all numerical ranges that are better within the broader numerical limits, as if the narrower numerical limitations were expressly written.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided a pharmaceutical composition for preventing hair loss and promoting hair growth comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients.

The pharmaceutical composition is excellent in biosafety as well as hair loss prevention and hair growth promoting activity by including a natural-derived complex extract as an active ingredient.

In particular, since the complex extract acts in a complex way in the human body, the effect of preventing hair loss and promoting hair growth is remarkably superior as compared to a single extract.

In addition, the pharmaceutical composition not only effectively promotes hair growth, but also has excellent anti-inflammatory, antibacterial activity and cell proliferation ability, and thus has excellent anti-dandruff and scalp inflammation relief.

The "rye ( Secale cereale )" is also called ribori, and is native to the Caucasus and Turkey. The rye leaves are greenish-blue, and velvety hairs are densely grown on the upper surface of the young leaves. Since the rye contains a large amount of dietary fiber, it gives a feeling of satiety to the consumer and is effective for dieting due to low calorie. The rye contains not only carbohydrates, but also protein, potassium, and vitamin B, thereby facilitating digestion and preventing constipation.

The rye extract (Rye extract) may be obtained by extracting the seeds or bran of rye, but the portion is not particularly limited.

The "flax ( Linum usitatissimum )" is a plant of the Linaceae family, which is native to the highlands of Central Asia.

The flaxseed is a seed of a one-year-old nut plant, the stem is 70 to 100 cm, while the root reaches 3 m, and because of the growth conditions that require a large amount of organic substances, once cultivated, omega-3 is not cultivated continuously for 7 years. , dietary fiber, lignin tocopherol, folic acid, minerals, etc. are concentrated.

The efficacy of flaxseed includes prevention of various vascular and heart diseases, prevention and treatment of stroke, anticancer effect, skin diseases such as atopic dermatitis, and brain growth promotion, and the flaxseed extract contains omega-3 fatty acids, omega- It may be flaxseed oil, which contains high amounts of 6 fatty acids, omega-9 fatty acids and saturated fatty acids.

The "wheat germ" is a nutrient-rich embryo of a wheat kernel removed in a process of processing whole wheat into flour, and accounts for about 2 to 3% of the total wheat kernel.

Wheat germ, the seed of the wheat ( Triticum vulgare ), is known to be rich in vitamin E (tocopherol), which is known as an antioxidant vitamin so far, and according to a recent study, a natural substance extracted from wheat germ has the effect of restoring the damaged immune function of the human body. is known to be

The wheat germ extract may be a conventional wheat germ oil obtained by pressing or extracting wheat germ.

The "Borridge ( Borrago officinalis )" is a borage native to Syria and is currently widely cultivated in North Africa, the Middle East, Europe, South America, and the Mediterranean region.

The borage is used in traditional medicine to relieve inflammation and pain, such as cough and sore throat, and has been used for more than 1500 years for cooking in everyday life. Currently, in Europe, borage is mainly cultivated for healing purposes and used in salads, soups, etc., or as a health supplement. The borage is known to have excellent anti-inflammatory activity, skin moisturizing, and regenerating effects, and to help relieve premenstrual syndrome and menopausal symptoms.

The borage extract may be obtained by extracting various parts of barley, but specifically may be a conventional vegetable oil extracted by pressing the seed of barley.

The "extract" means that the active ingredient contained in the extraction material is separated by contacting the solvent and the extraction material under specific conditions.

The complex extract may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, and the alcohol is 60 to It may be ethanol at a concentration of 90% (v/v).

The active ingredient contained in the raw material may have a different extraction ratio depending on the polarity of the solvent, and since the ethanol has excellent selectivity in extracting physiologically active substances from natural raw materials, the optimal hair growth promoting effect may be realized by the ethanol extraction. can

In particular, since water and ethanol have different polarities, active ingredients extracted according to each polarity may vary, and the concentration of ethanol may be appropriately controlled so that an optimal hair growth promoting effect can be realized.

If the concentration of the ethanol is more than 90% (w / w), an appropriate yield may not be realized, and if the concentration is less than 60% (w / w), the active ingredient for promoting hair growth may not be sufficiently extracted.

The complex extract is obtained by washing each raw material with water, then drying and pulverizing it, and using a solvent with a volume equivalent to 8 to 12 times the weight of the raw material, reflux circulation extraction, pressure extraction, ultrasonic extraction, etc. for about 1 hour to 24 hours. It can be prepared by extraction and filtration. In addition, the extract may be obtained in a powder state by an additional process such as distillation under reduced pressure or freeze-drying.

The extract may also include an extract that has undergone a conventional purification process. For example, the extract is separated using an ultrafiltration membrane having a constant molecular weight cut-off value, and various purification methods additionally performed such as separation by various chromatography (those prepared for separation according to size, charge, hydrophobicity or affinity). It may include a fraction obtained through

On the other hand, the pharmaceutical composition may further include a pine bark extract or saw palmetto extract.

The “pine tree” includes all kinds of pine trees such as red pine ( Pinus Densiflora ), sea pine ( Pinus maritima or Pinus pinaster ), new pine ( Pinus radiata ), gom pine ( Pinus thunbergii ), old pine tree ( Pinus sylvestris ), etc. can be used to mean

The pine bark can be divided into an inner bark and an outer bark, and both can be used.

The pine bark contains a large amount of natural polyphenol components, and according to a study on the French sea pine bark extract, the pine bark extract has an inhibitory effect on collagen degradation (Grimm et al., Free Rad Biol Med 36: 811- 822, 2004), blemish removal effect (Ni et al., Phytother Res 16: 567-571, 2002), skin elasticity enhancing effect (D. Segger and F. Schonlau, J. Dermatol. Treatment, 15: 1-5, 2004), edema improving effect (Arcangeli, Fitoterapia 71: 236-244, 2000), menstrual pain relief effect (Kohama & Suzuki, Eur Bull Drug Res, 7: 30-32, 1999), vasodilatory effect (D.F. Fitzpatrick et al. , J. Cardiovasc. Pharm. 32: 509-515, 1998) are known to have various physiological activities.

The " Serenoa repens " is a small palm tree with dark red fruits that is distributed along the Atlantic coast of North America, and usually grows in colonies. The saw palmetto fruit is a natural product that North American Indians have traditionally used for the purpose of preventing and treating urinary-genital diseases, etc., and its efficacy has been revealed through recent studies.

The active ingredients of the saw palmetto fruit are known as saponin, fatty acid, phytosterol, essential oil, lesin, tannin, etc., and the fruit is ingested or processed into an appropriate form and used. The saw palmetto is generally processed into standardized raw materials through extraction and used as pharmaceuticals or health functional foods.

The saw palmetto extract can be obtained by extracting various parts of the saw palm tree, preferably, it can be obtained by extracting the active ingredient of the saw palmetto fruit.

On the other hand, the pharmaceutical composition may further include a Moringa extract and biotin.

The "Moringa ( Moringa oleifera )" is one of the angiosperms of the Cruciferae family widely distributed in tropical and subtropical climates. The Moringa is an umbrella-like shrub with a height of 4 to 8 meters, leaves of 30 to 70 cm in length are deciduous, flowers are white and fragrant, and the fruit is a long triangular long pod of hyacinth and pendulum in length. 30 to 40 cm. Seed curved triangular, 1.2 cm long and 1 cm wide, with three membranous wings 2 cm long extending from the seed.

The Moringa tree can survive for months without water due to its very fast growing deep roots that survive even in extreme conditions.

The Moringa extract may be obtained from the outpost or various organs of Moringa (eg, leaves, flowers, stems, roots and fruits (seeds), etc.), preferably the outpost or fruit (seed) of Moringa, most preferably It can be obtained by extracting the forage.

The “biotin” is a vitamin containing sulfur, found naturally in many foods such as intestines, eggs, and certain vegetables, and is involved in fat and carbohydrate metabolism.

The biotin is referred to as 'vitamin H' because it has a good effect on the skin and hair, and is known not only to be involved in the production of blood cells and male hormone secretion, but also to facilitate the functions of the nervous system and bone marrow together with other B vitamins. have.

The pharmaceutical composition may include a pharmaceutically acceptable carrier in addition to the active ingredient.

The pharmaceutically acceptable carriers are commonly used in formulations, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, and mineral oil.

In addition, the pharmaceutical composition may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like, in addition to the above components.

The pharmaceutical composition may be administered orally or parenterally (eg, intravenously, subcutaneously, intraperitoneally or topically) according to a desired method, and the dosage may vary depending on the condition and weight of the patient, the degree of disease, the drug Although depending on the form, administration route and time, it may be appropriately selected by those skilled in the art.

The pharmaceutical composition may be administered in a pharmaceutically effective amount. The “pharmaceutically effective amount” means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is dependent on the patient's type, severity, drug activity, and drug Sensitivity, administration time, administration route and excretion rate, duration of treatment, factors including concomitant drugs, and other factors well known in the medical field may be determined.

The pharmaceutical composition may be administered as an individual therapeutic agent or may be administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered single or multiple.

In consideration of all of the above factors, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, which can be easily determined by those skilled in the art.

Specifically, the effective amount of the pharmaceutical composition may vary depending on the patient's age, sex, condition, body weight, absorption of the active ingredient into the body, inactivation rate and excretion rate, disease type, and drugs used in combination, and generally per 1 kg of body weight. 0.001 to 150 mg, preferably 0.01 to 100 mg, may be administered daily or every other day, or may be administered in divided doses 1 to 3 times a day.

However, since it may increase or decrease depending on the route of administration, the severity of obesity, sex, weight, age, etc., the dosage does not limit the scope of the present invention in any way.

According to another aspect of the present invention, there is provided a food composition for preventing hair loss and promoting hair growth comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients.

The "food composition" can be used as a concept including all of the conventional functional food or health functional food.

The functional food may include normal food additives, and unless otherwise specified, the food additives are approved by the Ministry of Food and Drug Safety according to the standards and standards for the relevant items in accordance with the general rules and general test methods of the Food Additives Code. suitability can be judged.

The items described in the Food Additives Codex include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; Mixed agents, such as an additive alkali agent, a preservative agent, and a tar dye agent, are mentioned.

The functional food can be variously used in foods and beverages for the improvement of antioxidants, hair loss, inflammatory diseases, etc. For example, it can be used in various foods, beverages, gum, tea, vitamin complexes, health functional supplements, food additives, etc. have.

The functional food may be manufactured and processed into any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and pills for the purpose of improving antioxidant, hair loss, inflammatory diseases, and the like.

Specifically, the functional food in tablet form is granulated by a conventional method by granulating a mixture with a plant extract or a fraction thereof, an excipient, a binder, a disintegrant, and other additives, and then compression molding by putting a lubricant or the like, or directly molding the mixture It can be manufactured by compression molding. In addition, the functional food in the form of tablets may contain a mating agent, etc. if necessary, and may be coated with a suitable skinning agent if necessary.

Among the functional foods in the form of capsules, hard capsules can be prepared by filling conventional hard capsules with a mixture of eggplant extract and additives such as excipients, or their granules or skinned granules, and soft capsules are plant extracts or fractions thereof. , and a mixture with additives such as excipients can be prepared by filling a capsule base such as gelatin. The soft capsules may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary.

The functional food in the form of a ring can be prepared by molding a mixture of plant extracts or fractions thereof, excipients, binders, disintegrants, etc. in an appropriate way, and, if necessary, coating the skin with sucrose or other suitable skinning agent, or starch, talc or You can also dress the gown with a suitable material.

The functional food in the form of granules may be prepared in granular form by a suitable method by preparing a mixture of plant extracts or fractions thereof, excipients, binders, disintegrants, etc., and may contain flavoring agents, corrosive agents, etc.

In addition, the term definitions for the excipients, binders, disintegrants, lubricants, flavoring agents, and the like are described in documents known in the art and may include those having the same or similar functions.

The health functional food refers to a food manufactured and processed using raw materials or ingredients useful for the human body according to the Health Functional Food Act No. 6727, and controls nutrients or physiological action for the structure and function of the human body. It refers to ingestion for the purpose of obtaining useful effects for health purposes such as

The health functional food may contain normal food additives, and unless otherwise specified, whether it is suitable as a food additive is specified in accordance with the general rules and general test methods of food additives approved by the Ministry of Food and Drug Safety. And it can be determined by the standard.

The health functional food is characterized in that it is formulated with one selected from the group consisting of tablets, pills, powders, granules, powders, capsules and liquid formulations, including one or more of carriers, diluents, excipients and additives.

Foods that can be added to the health functional food include various foods, powders, granules, tablets, capsules, syrups, beverages, gums, tea, vitamin complexes, health functional foods, and the like.

Additives that may be further included in the health functional food include natural carbohydrates, flavoring agents, nutrients, vitamins, minerals (electrolytes), flavoring agents (synthetic flavoring agents, natural flavoring agents, etc.), coloring agents, fillers, lactic acid and its At least one component selected from the group consisting of salts, monoacids and salts thereof, organic acids, protective colloidal thickeners, PH regulators, stabilizers, preservatives, antioxidants, glycerin, alcohols, carbonation agents, and pulp may be used.

Examples of the natural carbohydrate include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol.

As the flavoring agent, natural flavoring agents (taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used.

In addition, the health functional food contains various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and thickening agents, facic acid and salts thereof, alginic acid and salts thereof, organic acids, protective properties colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like.

Specific examples of the carrier, excipient, diluent and additive include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, erythritol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium phosphate, calcium silicate , microcrystalline cellulose, polyvinylkyrolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water, sugar syrup, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate and mineral oil. At least one selected from the group is preferably used, but is not limited thereto.

According to another aspect of the present invention, there is provided a cosmetic composition for preventing hair loss and promoting hair growth comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients.

The cosmetic composition may further include an amino acid complex, hydrolyzed silk, a flavoring agent, ceramide, and an excipient.

The amino acid complex refers to a complex composition in which one or more amino acids, which are basic structural units of a protein, are mixed, and the type or mixing ratio of the amino acids to be mixed is not particularly limited, and may be changed as necessary. The amino acid complex provides moisture to the skin and gives shine to the hair, so it is possible to enhance the feeling of use of the composition and minimize irritation to the skin.

The hydrolyzed silk (hydrolyzed silk) refers to water or dilute ethanol containing a peptide obtained by hydrolysis of silk fibers with acid or alkali, and can often be used as a skin or hair conditioning agent. The hydrolyzed silk is safe to the human body as a component of protein, and it can give shine and elasticity to the hair and restore damaged hair.

The flavoring agent refers to a substance added to food or cosmetics to give a fragrance, and has excellent volatility at room temperature, and may be added as necessary for commercialization of the composition. The type of the flavoring agent is not particularly limited, and aromatic chemicals, essential oils, natural extracts, distillates, isolated components, aromas, resinous resins, and the like may be used.

The ceramide occupies about 40% or more of the lipid between the keratinocytes constituting the stratum corneum of the skin and is an essential component for the formation or function of the structure of the stratum corneum. can be suppressed. Since the ceramide forms a moisture film on the hair to inhibit moisture evaporation and prevent damage, it may be added as a raw material for a moisturizer.

The excipien refers to a substance added to give a drug an appropriate hardness or shape, or to give a certain amount and weight when the amount of the main agent is insufficient to make it a size that is easy to handle. is not particularly limited, lactose, starch, dextrin, glycerin, cellulose, etc. may be used.

The cosmetic composition may include various additives such as emulsifiers, preservatives, diluents, and pigments in consideration of the function or use of the composition as well as the additives.

The cosmetic composition may further include a base component that can be formulated in general external preparations for scalp and hair, and specifically, a solubilizing agent, a surfactant, a moisturizer, a thickener, a pH adjuster, a preservative, an antioxidant, a sequestering agent, fungicides, anti-inflammatory agents, antimicrobial agents, solvents, coloring agents, or flavoring agents, and the like.

The solubilizing agent may be isopropyl myristate, polyethylene glycol, medium-chain fatty acid triglycerides, hydrocarbons, glycols, and the like.

Among the surfactants, anionic surfactants include ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl sesionate, sodium lauryl isesionate, sodium lauryl sulfate, triethanolamine lauryl sulfate, and sodium lauryl sulfate. uryl ether sulfate (1 to 3 ethylene oxide) and the like can be used. Nonionic surfactants may include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene, hydrogenated castor oil derivative, fatty acid diethanolamide, glyceryl stearate, and the like. Cationic surfactants may include tertiary aliphatic amine salts, alkyltrimethylammonium chloride, dialkyldimethylammonium chloride, and the like. Specifically, the amphoteric surfactant may be betaine, amide betaine, sulfobetaine, steartrimonium chloride, or the like.

The moisturizing agent may include glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, and the like.

As the thickener, a water-soluble polymer compound such as methylcellulose, hydroxyethylcellulose, carrageenan, carboxymethylcellulose, and hydroxymethylcellulose may be used.

Specifically, the pH adjuster may include citric acid, sodium hydroxide, triethanolamine, sodium citrate, phosphoric acid, sodium phosphate, lactic acid, and the like.

The preservative may be benzoic acid, paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol, DMDMhydantoin, etc., and the antioxidant may be dibutylhydroxytoluene, ascorbic acid, etc. .

The sequestering agent may include disodium ethylenediaminetetraacetate, tetrasodium ethylenediaminetetraacetate, and the like, and the disinfectant is chlorhexidine gluconate, quaternary ammonium salt, pyroctonolamine, zinc pyrithione suspension, iodopropynyl butyl. Carbamates, salicylic acid, and the like can be used.

The anti-inflammatory agent may include monoammonium glycyrrhizinate, dipotassium glycyrrhizinate, stearyl glycyrrhizinate, chamomile, alpha-bisabolol, allantoin, and the like, and the antimicrobial agent is specifically phenoxyethanol, chlorhexidine, chlorhexidine gluconate, and pyroctonol. Amine, ketoconazole, arnica extract, iodopropynyl butyl carbamate, benzalkonium chloride, benzenetonium chloride, benzoic acid and its salts, benzyl alcohol, lavender, rosemary, salicylic acid, triclocarban, zinc pyrithione suspension, etc. can be used.

As the solvent, ethanol, purified water, Tween 20, cyclomethicone, mineral oil, dimethicone, and the like may be used, and the colorant and flavoring agent may be conventional bases used in scalp and hair preparations.

On the other hand, the composition may be formulated as a composition for hair treatment such as emulsion, cream, paste, gel, lotion, pack, lotion, powder, spray, soap, etc., specifically, hair toner, hair lotion, hair cream, hair spray. , hair mousse, hair gel, hair soap, hair shampoo, hair conditioner, hair pack, and hair treatment may be formulated with one or more selected from the group consisting of, and specifically formulated as a liquid hair shampoo.

In the formulation, other components in addition to the above-described essential components may be appropriately selected and formulated by those skilled in the art according to the use or purpose of use of other external preparations.

For example, when the formulation of the composition is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as a carrier component. can be used

When the formulation of the composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, additionally chlorofluorohydrocarbon, propane, butane or It may further contain a propellant such as dimethyl ether.

When the formulation of the composition is a solution or emulsion, a solvent, solvating agent or emulsifying agent may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the formulation of the composition is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

Through the following examples, the present invention will be described in more detail, but it is obvious that the present invention is not limited by the following examples.

Preparation Example: Preparation of natural extract

Rye, pine bark, and moringa were each washed with water, dried completely at room temperature, and 100 g of a pulverized product was obtained.

For 100 g of each pulverized product, 20-fold volume of ethanol was used as a solvent and immersion extraction was performed at a temperature of 50° C. for 24 hours.

Flaxseed, barley, wheat germ, and saw palmetto fruit were each dried and then physically compressed to obtain an extract in the form of an oil.

The extract was filtered through a 250 mesh and 3 μm filter, and the remaining raw materials were extracted three times in the same manner, concentrated under reduced pressure at 50° C., and freeze-dried to obtain a solid content.

Examples and Comparative Examples

Examples and Comparative Examples were set by mixing samples as shown in Tables 1 and 2 below to verify the stability and functionality of the obtained extract.

[Content (parts by weight)] division Example One 2 3 4 5 6 rye extract 30 24 24 20 20 20 Flaxseed Extract 30 24 24 20 20 20 wheat germ extract 30 24 24 20 20 20 borage extract 30 24 24 20 20 20 Pine Bark Extract - 24 - 20 15 15 Saw Palmetto Extract - - 24 20 15 15 Moringa Extract - - - - 10 5 biotin - - - - - 5

[Content (parts by weight)] division comparative example One 2 3 4 5 6 7 8 9 10 11 12 rye extract 120 - - - - - - - 40 - 40 - Flaxseed Extract - 120 - - - - - - 40 40 40 - wheat germ extract - - 120 - - - - - 40 40 - - borage extract - - - 120 - - - - - 40 40 - Pine Bark Extract - - - - 120 - - - - - - 40 Saw Palmetto Extract - - - - - 120 - - - - - 40 Moringa Extract - - - - - - 120 - - - - 40 biotin - - - - - - - 120 - - - -

Experimental Example 1: Skin safety evaluation

In order to verify the skin safety of the samples of Examples and Comparative Examples, an MTT assay experiment on fibroblasts (HDF) was performed.

After each of the samples of Examples and Comparative Examples were suspended in purified water for each concentration of 25, 50, 100, 200, and 400 μg/mL, cell viability was measured.

Cytotoxicity was performed by modifying Mosmann's method for measuring cell viability using MTT {3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide} reagent.

Fibroblasts (HDF) were each dispensed in a 96-well plate at a concentration of 1×10 ⁴ cells/well and cultured at 37° C., 5% CO ₂ for 48 hours.

After removing the culture medium and culturing the sample in a medium treated for each concentration for 48 hours, the medium was removed and washed repeatedly with PBS (Phosphate buffered saline).

MTT was dissolved in PBS at 5 mg/mL, 50 μL was added, and incubated at 37° C., 5% CO ₂ for 48 hours. 100 L of DMSO (dimethyl sulfoxide) was added per well, and the absorbance was measured at 540 nm after stirring for 10 minutes.

As a result of the measurement, cytotoxicity was not confirmed at all concentrations of each sample. These results suggest that the sample is not only harmless to the human body, but also has excellent human safety.

Experimental Example 2: Evaluation of the effect of promoting dermal papilla cell proliferation

The dermal papilla cells of Examples and Comparative Examples using PVG Rat Vibrissa dermal papillae cells prepared by transfection of a polyomavirus large T gene encoding a temperature-sensitive T antigen The proliferation promoting effect was evaluated.

The dermal papilla cells were cultured in a 6-well plate at a concentration of about 10 ⁴ cells/well per well.

The samples of Examples and Comparative Examples were dissolved in DMSO (dimethyl sulfoxide) or ethanol, respectively, and then serially diluted with DMEM medium with 5% FBS serum concentration to adjust the final concentration to 10 ppm.

After adding 200 μL of the medium to which the samples of Examples and Comparative Examples were added per well, incubated for about 3 days in a 37° C. 5% CO ₂ incubator, after adding 50 μL of MTT solution to each well, 4 hours in the same incubator incubated for more.

After the MTT reagent was absorbed into the intracellular mitochondria, the supernatant was removed, 150 μL of DMSO was added, and stirred for 10 minutes to uniformly dissolve the formazan dye (dye).

O.D. using an ELISA reader at 515 nm wavelength. The value was measured, and the number of cells was measured according to a standard curve prepared in advance.

Based on the measured cell number, the dermal papilla cell proliferation promoting ability (%) of Examples and Comparative Examples was calculated. The evaluation results are shown in Table 3 below.

division Cell proliferation promoting effect (%) Example 1 57.9±1.1 Example 2 63.6±1.3 Example 3 62.2±1.2 Example 4 65.4±1.1 Example 5 67.2±1.2 Example 6 69.1±1.1 Comparative Example 1 22.1±1.3 Comparative Example 2 18.2±1.2 Comparative Example 3 18.2±0.9 Comparative Example 4 20.3±1.3 Comparative Example 5 22.5±1.0 Comparative Example 6 19.4±1.2 Comparative Example 7 21.5±1.0 Comparative Example 8 23.8±1.0 Comparative Example 9 36.2±0.9 Comparative Example 10 32.5±1.1 Comparative Example 11 34.9±1.0 Comparative Example 12 29.5±1.1

Referring to Table 3, the mixed samples of Examples exhibited a significantly superior effect on promoting proliferation of flow cells compared to the samples of Comparative Examples, and a synergistic effect was identified according to the complex application of the samples. In particular, Examples 2 to 6 Since the sample further promoted cell proliferation compared to Example 1, it can be evaluated that the hair loss prevention and hair growth promoting effect are very excellent.

Experimental Example 3: Evaluation of capillary dilation effect

In order to evaluate the capillary dilatation effect of the samples of Examples and Comparative Examples, the degree of proliferation of fibroblasts was measured.

Potassium channel is activated to promote mitogenesis, relax vascular smooth muscle to induce vasodilation, while blocking promotes proliferation of NIH3T3 fibroblasts, so fibroblast proliferation By evaluating the degree, it is possible to check whether the potassium channel is open.

To evaluate the capillary dilatation capacity of the sample, NIH3T3 fibroblasts were seeded in a 24-well culture plate at a concentration of 10 ⁴ cells/well, and 10% fetal calf serum (FCS) and 2mM L-glutamine were included. and cultured in DMEM medium without antibiotics for 24 hours.

On the other hand, the cultured medium was replaced with each medium to which the extracts of Examples and Comparative Examples prepared in the same manner as in Experimental Example 1 were added, and further cultured for 72 hours.

At this time, after removing the medium, it was washed with PBS (Ca ²⁺ /Mg ²⁺ no), treated with 0.5ml TE (trypsin-ethylenediamine tetraacetic acid), and then cultured.

DMEM containing 10% (v/v) FCS was added and the cells were separated, placed in an EP tube, and centrifuged at 5500 rpm.

After removing the supernatant, the remaining cell solution was suspended in 100 μL of PBS, and the number of cells was counted using a hemocytometer.

The capillary dilatation effects of Examples and Comparative Examples measured according to the above results are shown in Table 4 below.

division Capillary dilatation effect (%) Example 1 51.5±2.1 Example 2 57.3±1.4 Example 3 56.9±1.6 Example 4 61.2±1.6 Example 5 63.7±1.8 Example 6 66.1±1.7 Comparative Example 1 21.1±1.2 Comparative Example 2 18.7±1.1 Comparative Example 3 23.4±1.3 Comparative Example 4 27.5±1.4 Comparative Example 5 26.3±1.2 Comparative Example 6 23.5±1.3 Comparative Example 7 29.2±1.4 Comparative Example 8 24.9±1.1 Comparative Example 9 31.2±1.3 Comparative Example 10 32.9±1.1 Comparative Example 11 33.5±1.4 Comparative Example 12 30.9±1.2

Referring to Table 4, the mixed sample of Example was significantly superior to the capillary dilatation effect compared to the sample of Comparative Example. In particular, since the samples of Examples 2 to 6 had better capillary dilatation effect compared to Example 1, it is evaluated that it is possible to improve the blood circulation of the scalp capillaries to promote hair health and inhibit the progress of hair loss. .

Experimental Example 4: Evaluation of hair growth promoting activity

After hair removal of about 50-day-old mice (C57BL/6) in which the hair entered the telogen phase, the hair growth promoting effect of Examples and Comparative Examples was evaluated.

In this experiment, mice with similar characteristics such as weight and size were divided into 18 groups of 5 each, and then individually bred to measure the degree of hair growth.

After ingestion of a cereal feed containing 1.0% by weight of the samples of Examples and Comparative Examples, they were bred for 30 days, and the hairs grown in the hair removal area were separated and the weight was measured.

The hair growth promoting effect of the extract was more clearly evaluated by setting the mice fed the cereal feed not containing the samples of Examples and Comparative Examples as a control group. The measurement results are shown in Table 5 below.

division Hair weight (mg) Example 1 236.1±11.8 Example 2 256.9±12.3 Example 3 254.2±12.1 Example 4 269.5±11.5 Example 5 278.2±13.1 Example 6 284.8±12.6 Comparative Example 1 112.9±8.1 Comparative Example 2 99.6±9.3 Comparative Example 3 95.2±7.6 Comparative Example 4 104.3±9.6 Comparative Example 5 102.6±8.4 Comparative Example 6 113.2±8.6 Comparative Example 7 121.5±9.1 Comparative Example 8 119.5±9.4 Comparative Example 9 143.2±8.9 Comparative Example 10 135.5±9.0 Comparative Example 11 133.9±10.2 Comparative Example 12 129.1±9.3 control 53.7±6.5

Referring to Table 5, the mixed sample of Example was significantly superior to the hair growth promoting effect compared to the sample of Comparative Example.

In particular, the samples of Examples 2 to 6 were evaluated to be able to promote hair health and inhibit hair loss because the hair growth promoting effect was more excellent compared to Example 1.

Experimental Example 5: Evaluation of free radical scavenging activity

The extracts of Examples and Comparative Examples were suspended in purified water (concentration of 10%) to evaluate free radical scavenging activity.

The DPPH measurement method is a method of measuring the absorbance at 540 nm to determine the extent to which an inhibitor is decolorized by scavenging a stable radical DPPH (2,2-diphenyl-1-picrylhydrazyl radical).

The free radical scavenging activity was clearly compared using dibutyl hydroxy toluene (BHT) as a control.

Dibutylhydroxytoluene is a colorless crystal or white crystalline powder and has an excellent antioxidant effect and is widely used as an antioxidant in edible oils and fats.

The same experiment was repeated 3 times for the accuracy of the experiment, and the results are shown in Table 6 below.

division DPPH radical scavenging activity (%) Example 1 146.5 Example 2 153.7 Example 3 151.9 Example 4 158.4 Example 5 163.2 Example 6 167.6 Comparative Example 1 102.2 Comparative Example 2 103.3 Comparative Example 3 104.5 Comparative Example 4 104.7 Comparative Example 5 102.8 Comparative Example 6 106.4 Comparative Example 7 103.9 Comparative Example 8 105.1 Comparative Example 9 112.3 Comparative Example 10 115.8 Comparative Example 11 109.7 Comparative Example 12 114.4 control 161.5

Referring to Table 6, the free radical scavenging activity of the sample of Example increased as the concentration increased, and the free radical scavenging activity was significantly superior to that of the sample of Comparative Example. The higher the free radical scavenging activity, the better the antioxidant effect. can be evaluated,

In particular, the samples of Examples 2 to 6 were more excellent in antioxidant effect than in Example 1.

Experimental Example 6: Evaluation of anti-inflammatory activity

The anti-inflammatory activity of Examples and Comparative Examples was evaluated by analyzing the NO production inhibitory ability of EAME in the rat macrophage RAW 264.7 cell induced by lipopolysaccharide (LPS) stimulation.

For the experiment, Raw 264.7 cells, a murine macrophage cell line, were distributed from the Korean cell line bank and cultured in a 5% CO ₂ incubator at 37°C using DMEM medium containing 100 units/mL penicillin-streptomycin and 10% fetal bovine serum (FBS). and subculture was performed once every 2-3 days.

Raw 264.7 cells (3 X 10 ⁵ cells/mL) were cultured 18 hours before, and the samples of Examples and Comparative Examples (concentration 10%) and LPS (1 μg/mL) were simultaneously treated and cultured for 24 hours.

The generated NO was measured in the form of NO ₂ ⁻ present in the cell culture medium using Griess reagent.

The negative control group was not treated with LPS, and the positive control group was treated with only LPS to measure the amount of NO generated.

100 μL of cell culture supernatant and Griess reagent (1% (w/v) sulfanilamide, 0.1% (w/v) naphtylehtylenediamine in 2.5% (v/v) phosphoric acid) were mixed in equal amounts and reacted in the dark at room temperature for 10 minutes, followed by ELISA Absorbance (540 nm) was measured using a reader.

The standard concentration curve was obtained by serial dilution of sodium nitrite (NaNO ₂ ) (10-100 μM).

division NO production (μM) Example 1 15.35 Example 2 13.75 Example 3 12.42 Example 4 11.53 Example 5 10.93 Example 6 9.85 Comparative Example 1 26.34 Comparative Example 2 24.78 Comparative Example 3 25.28 Comparative Example 4 28.34 Comparative Example 5 26.61 Comparative Example 6 27.43 Comparative Example 7 25.21 Comparative Example 8 24.52 Comparative Example 9 23.31 Comparative Example 10 22.59 Comparative Example 11 23.52 Comparative Example 12 24.25 LPS(-) 6.53 LPS(+) 37.24

Referring to Table 7, the mixed sample of Example exhibited a higher NO (nitric oxide) production inhibitory activity as compared to the sample of Comparative Example, and was remarkably excellent in antioxidant and anti-inflammatory activity.

Experimental Example 7: Evaluation of antibacterial activity

The antibacterial activity of the samples of Examples and Comparative Examples was evaluated. Pityrosporum ovale ATCC 12087 was used as a test bacteria, and triclosan, a chemically synthesized antibacterial agent, was used as a positive control.

Propionibacterium acnes was struck on tryptic soy agar medium containing 5% sheep blood and cultured for 2 to 3 days in advance, prepared by McFaland nephleometer standards 0.5 (1% Barum chloride (0.05 ml) + 1% Sulfuric acid). (9.95 ml)), the concentration of bacteria was 1x10 ⁸ cells/mL.

After diluting each sample in 2ml of liquid BHI medium at intervals of 100ppm from 0 to 2000ppm, inoculated with 20μL of 1x10 ⁸ cells/mL bacterial solution and incubated for 48 hours.

Based on the turbidity, the minimum concentration inhibiting growth was defined as the minimum growth inhibitory concentration (MIC), and the results are shown in Table 8 below.

division Minimum growth inhibitory concentration MIC (PPM) Example 1 620 Example 2 580 Example 3 570 Example 4 540 Example 5 520 Example 6 490 Comparative Example 1 1220 Comparative Example 2 1370 Comparative Example 3 1140 Comparative Example 4 1520 Comparative Example 5 1470 Comparative Example 6 1230 Comparative Example 7 1360 Comparative Example 8 1120 Comparative Example 9 1280 Comparative Example 10 970 Comparative Example 11 1060 Comparative Example 12 1020 triclosan 450

Referring to Table 8, the mixed sample of Example had a rather weak antibacterial effect compared to triclosan, a positive control, but had significantly superior antibacterial effect compared to the sample of Comparative Example.

In particular, the samples of Examples 2 to 6 were more excellent in the antibacterial effect against dandruff compared to Example 1.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Claims (9)

A pharmaceutical composition for preventing hair loss and promoting hair growth comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients. According to claim 1,
A pharmaceutical composition further comprising a pine bark extract or saw palmetto extract. 3. The method of claim 2,
A pharmaceutical composition further comprising a Moringa extract. 4. The method of claim 3,
A pharmaceutical composition further comprising biotin. delete A food composition for preventing hair loss and promoting hair growth comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients. 7. The method of claim 6,
A food composition further comprising a pine bark extract or saw palmetto extract. A cosmetic composition for preventing hair loss and promoting hair growth, comprising rye extract wheat, flaxseed extract, wheat germ extract, and borage extract as active ingredients. 9. The method of claim 8,
A cosmetic composition formulated with one or more selected from the group consisting of hair toner, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair soap, hair shampoo, hair rinse, hair pack, and hair treatment.