KR102358623B1 - Food composition for improving skin condition comprising collagen peptide - Google Patents

Abstract

The present invention relates to a food composition for skin condition improvement containing collagen peptide, vitamin C, hyaluronic acid, and elastin as active ingredients, in which the collagen peptide has a glycine-proline-hydroxyproline (GPH) content of 3.2 wt% or more. The internal absorption rate of the food composition of the present invention is very high even in the case of oral administration. Accordingly, the composition has various skin activity improvement functions such as skin revitalization, skin vitality enhancement, skin convergence, wrinkle improvement, skin barrier strengthening or barrier function protection, skin regeneration, moisturizing, elasticity maintenance, anti-oxidation, anti-aging, pore contraction, and sebum inhibition.

Description

Food composition for improving skin condition comprising collagen peptide

The present invention relates to a food composition comprising a collagen peptide and having excellent skin improvement effect due to excellent absorption of collagen in the body when ingested.

As the human skin ages, the secretion of various hormones that internally control the metabolism decreases, and the functions and activities of immune cells and skin cells are lowered. Intrinsic aging occurs.

In addition, externally, aging occurs due to the generation of blemishes, freckles and dry skin due to increased wrinkles, decreased elasticity, and increased skin dryness due to photoaging caused by various pollutants and ultraviolet rays.

The skin is composed of the epidermis, dermis, and subcutaneous tissue. Among them, the dermis is composed of fibrous and matrix components, and collagen, which occupies most of the dermis, provides strength and tension to the skin and protects the skin. .

As we age, the amount of fibroblasts (collagen) synthesized decreases due to the action of fibroblasts, which synthesize and secrete fibrous components in the matrix of connective tissue, and the decrease in the number of cells, and moisture in the skin cells is lost, and the structure of the stratum corneum will change

In addition, the action of collagenase, which decomposes collagen, which is a major structural component of the skin, increases, and the cross-linked form of collagen increases, thereby reducing smoothness, moisture, and firmness.

Recently, as consumers' aesthetic desire for anti-aging rises, consumers' desire to purchase products containing anti-aging and wrinkle-improving ingredients is increasing. has become a major concern in relation to skin beauty and aging inhibition, and there is a demand for the development of beauty foods that can enhance collagen synthesis while suppressing photoaging by UV.

In addition to cosmetic compositions applied to the skin, research on collagen that can be ingested orally has been continuously conducted, and collagen with various sources and collagen raw materials that have undergone various post-processing processes have been developed as technology advances. In this regard, Korean Patent Application Laid-Open No. 2001-0075842 (Aug. 11, 2001) discloses a "functional food" containing collagen, and Korean Patent Publication No. 2002-0085307 (Jan. 16, 2002) discloses "Oral skin" A composition containing a large amount of collagen is disclosed as "cosmetic food composition". However, since collagen itself has a large molecular weight, various questions arise such as problems with digestion and absorption, delivery to target organs such as skin, reaching an effective amount, and compatibility of living collagen. the current situation.

The present invention is to solve the above problems, and it is intended to provide a food composition containing collagen peptides, which has excellent absorption rate of collagen in the body and excellent skin improvement effect when ingested, and is safe and has no side effects.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those of ordinary skill in the art from the following description.

According to one embodiment of the present invention, it contains collagen peptide, vitamin C, hyaluronic acid and elastin as active ingredients, and the collagen peptide has a glycine-proline-hydroxyproline (GPH) content of 3.2 weight % or more.

In the present invention, the collagen peptide may have a molecular weight of 200 to 500 Da.

In the present invention, the collagen peptide may be extracted from fish.

In the present invention, the collagen peptide may be included in an amount of 6,000 mg or more.

In the present invention, the collagen peptide is 15 to 25% by weight; Vitamin C is 0.001 to 0.50% by weight; hyaluronic acid is 0.001 to 0.50% by weight; and elastin may be included in an amount of 0.001 to 0.50% by weight.

In the present invention, the collagen peptide, vitamin C, hyaluronic acid and elastin may be included in a weight ratio of 1,000 to 5,000: 1 to 5: 1 to 5: 1 to 5, respectively.

In the present invention, the food composition may further include one or more selected from the group consisting of yeast, propolis, N-acetylglucosamine and biotin.

In the present invention, the yeast may contain 1.5 wt% or more of glutathione.

In the present invention, one or more selected from the group consisting of yeast, propolis, N-acetylglucosamine, and biotin may be included in an amount of 0.001 to 0.50% by weight.

In the present invention, the food composition may further include one or more selected from the group consisting of milk ceramide, fermented horse placenta extract, and aloe vera.

In the present invention, at least one selected from the group consisting of milk ceramide, fermented horse placenta extract and aloe vera may be included in an amount of 0.001 to 0.50 wt%.

In the present invention, the food composition may further include one or more selected from the group consisting of mango extract, grapefruit extract and orange extract.

In the present invention, at least one selected from the group consisting of the mango extract, grapefruit extract and orange extract may be included in an amount of 5 to 20% by weight.

In the present invention, the food composition may include a solvent as the balance.

In the present invention, the food composition may be a powder, granules, tablets, capsules, pills, extracts, jelly formulations, tea bag formulations or beverage formulations.

In the present invention, the skin improvement is skin vitality, skin vitality enhancement, skin convergence, wrinkle improvement, skin barrier strengthening or barrier function protection, skin regeneration, moisturizing, elasticity maintenance, antioxidant, anti-aging, pore shrinkage or sebum suppression can

The food composition provided in the present invention has a very high absorption rate of collagen in the body even when administered orally, and thus provides skin vitality, skin vitality, skin convergence, wrinkle improvement, skin barrier strengthening or barrier function protection, skin regeneration, moisturizing, It has excellent benefits in improving various skin activities such as elasticity maintenance, antioxidant, anti-aging, pore contraction, and sebum suppression.

According to one embodiment of the present invention, it relates to a food composition for improving skin.

In the present invention, the "skin improvement" means skin vitality, skin vitality enhancement, skin convergence, wrinkle improvement, skin barrier strengthening or barrier function protection, skin regeneration, moisturizing, elasticity maintenance, antioxidant, anti-aging, pore contraction, sebum It contains various skin activity improvement functions such as inhibition.

collagen peptide

The food composition of the present invention may include a collagen peptide.

In the present invention, the collagen peptide is a complex comprising three amino acids, "glycine-x-y", wherein x and y may be any amino acid, and x and y may be the same or different amino acids. That is, alanine (alanine, Ala), valine (Val), leucine (Leu), isoleucine (Ileucine, Ile), proline (Proline, Pro), hydroxyproline (Hyp ), phenylalanine (Phe), tryptophan (Trp), methionine (Met), serine (serine, Ser), threonine (threonine, Thr), cysteine (cysteine, Cys), glutamine (glutamine, Gln) , glycine (Gly), asparagine (Asn), tyrosine (Tyr), lysine (Lys), arginine (Arg), histidine (Histidine, His), aspartic acid (aspartic acid, Asp) and amino acids such as glutamic acid (Glu) can be used, but preferably Gly-Pro-Hyp, Gly-Pro-Ala, Aly-Ala-Hyp, Gly-Leu-Hyp, Gly-Glu- It can be used in the composition of Lys, Gly-Pro-Lys, Gly-Glu-Hyp, Gly-Phe-Hyp, Gly-Ser-Hyp, Gly-Gln-Hyp and Gly-Glu-Arg, Gly-Pro-Arg, Preferably, glycine-proline-hydroxyproline (glycine-proline-hydroxyproline, GPH) may be linked.

In the present invention, when the collagen peptide has a GPH content of 3.2% by weight or more, preferably 3.6% by weight or more, it is advantageous in that the amount of absorption into the body is excellent when taken orally.

In addition, in the present invention, the collagen peptide has a very small molecular weight of 200 to 500 Da and can easily penetrate the skin. In the present invention, the collagen peptides are reconnected to each other immediately after skin penetration, thereby contributing to shortening the time to repair damaged collagen tissue.

In the present invention, the collagen peptide is extracted from fish, and is also called fish collagen or marine collagen, and has good absorption in the body, high stability, and excellent heat stability and acid resistance compared to animal collagen.

In the present invention, the method for producing the collagen peptide may be according to the method described in Korean Patent Publication No. 10-1831431, but is not limited thereto.

In the present invention, the collagen peptide may be included in an amount of 15 to 25% by weight, preferably 18 to 22% by weight, more preferably 20% by weight. In the present invention, when the content of the collagen peptide is less than 15% by weight, the skin improvement effect is insignificant, and when the content exceeds 25% by weight, formulation may be a problem.

In the present invention, the collagen peptide may be included in an amount of 6,000 mg or more. In the present invention, when the content of the collagen peptide is less than 6,000 mg, a sufficient skin improvement effect at a desired level cannot be obtained.

Vitamin C, hyaluronic acid and elastin

The food composition of the present invention can significantly increase the absorption of collagen peptides into the body by including at least one of vitamin C, hyaluronic acid, and elastin, preferably vitamin C, hyaluronic acid, and elastin.

In the present invention, by including the vitamin C in an amount of 0.001 to 0.50% by weight, preferably 0.005 to 0.010% by weight, more preferably 0.01 to 0.05% by weight, it is possible to increase the absorption and binding rate of collagen in the body. However, if it is out of the above content range, this desired effect cannot be achieved.

In the present invention, the hyaluronic acid may be included in an amount of 0.001 to 0.50% by weight, preferably 0.005 to 0.010% by weight, more preferably 0.01 to 0.05% by weight, and when it is outside the content range, the desired effect can be achieved. can't

In the present invention, the elastin may be included in an amount of 0.001 to 0.50% by weight, preferably 0.005 to 0.010% by weight, more preferably 0.01 to 0.05% by weight, and when out of the content range, the desired effect can be achieved. none.

In the present invention, the collagen peptide and vitamin C, hyaluronic acid and elastin are each in a weight ratio of 1,000 to 5,000: 1 to 5: 1 to 5: 1 to 5, preferably 1,500 to 2,500: 1 to 2: 1 to 2: Mixing in a weight ratio of 1 to 2 is preferable because it can significantly increase the absorption rate of the collagen peptide into the body.

Yeast, propolis, N-acetylglucosamine and biotin

The food composition of the present invention includes at least one selected from the group consisting of yeast, propolis, N-acetylglucosamine and biotin, preferably yeast, propolis, N-acetylglucosamine and biotin, thereby improving the absorption of collagen peptides into the body. Not only can it significantly increase, but it also gives synergy to the skin improvement effect of collagen, or in addition to it, there is an effect of enhancing skin immunity.

In the present invention, the yeast contains glutathione (L-γ-glutamyl-L-cysteinylglycine, GSH), and preferably contains the glutathione in an amount of 1.5% by weight or more, more preferably 2.5% by weight or more. It is desirable because it has the effect of promoting health and further improving liver function.

In the present invention, an extract of glutathione-containing yeast can be used as the yeast, and the method of obtaining the glutathione-containing yeast extract is not particularly limited, but for example, molasses is used as a medium and baker's yeast (Saccharomyces cerevisae) is inoculated and cultured. Then, the cells are separated from the culture solution and washed with water, the washed cells are extracted with hot water and filtered to remove sludge, the hot water extract is sterilized, dextrin is mixed, and spray-dried to prepare a powder.

In the present invention, the propolis is a substance in the form of a glue made by bees by mixing their own saliva with components collected from sprouts and bark of poplar trees, eucalyptus trees, pines, etc. It is a mixed substance.

In the present invention, the N-acetylglucosamine is a natural substance mainly present in the skeleton of crustaceans such as crab and shrimp, mushrooms, and mollusks, and is a precursor of hyaluronic acid, and has a function of promoting cell adhesion, proliferation and activity in the basal layer. . In the present invention, the N-acetylglucosamine is known to promote the production of hyaluronic acid, and plays an important role in moisturizing the skin in the dermal layer.

In the present invention, the biotin is a water-soluble vitamin B family, and is also called vitamin B7 (vitamin B7), vitamin B8 (vitamin B8), vitamin H (vitamin H), or coenzyme R (coenzyme R). Biotin is known to be involved in various metabolic processes in almost all living organisms, mainly related to fat, carbohydrate, and protein metabolism.

In the present invention, at least one selected from the group consisting of yeast, propolis, N-acetylglucosamine and biotin is 0.001 to 0.50% by weight, preferably 0.005 to 0.010% by weight, more preferably 0.01 to 0.10% by weight When included in an amount of less than 0.001% by weight, the desired synergistic effect cannot be imparted, and when included in an amount exceeding 0.50% by weight, the content of collagen peptide is relatively reduced or rather a synergistic effect is not exerted. There is a problem that it does not.

Milk Ceramide, Fermented Placenta Extract and Aloe Vera

The food composition of the present invention includes at least one selected from the group consisting of milk ceramide, fermented horse placenta extract and aloe vera, preferably milk ceramide, fermented horse placenta extract and aloe vera, thereby improving skin wrinkles, enhancing skin elasticity, It is possible to further improve the functionality of skin improvement, such as increasing skin moisture or skin whitening.

In the present invention, the milk ceramide is a mixture of milk phospholipids (lipids), carbohydrates, and proteins, characterized in that it contains milk-derived sphingomyelin. In the present invention, a commercially available milk ceramide (“Milk Ceramide MC-5” manufactured by Yukijirushi Megmilk Co., Ltd., etc.) can be used, but one manufactured using other known methods can also be used.

In the present invention, the method for preparing the milk ceramide is not particularly limited, but for example, butter serum or butter serum powder reducing solution is adjusted to pH 4.0 to 5.0, and calcium chloride is added to promote protein aggregation. , a method of drying the concentrated solution obtained by removing the generated precipitate and ultrafiltration or microfiltration of the supernatant (Japanese Patent Laid-Open No. 2007-89535), or adjusting the buttermilk or buttermilk powder reducing solution to an acidic region, The method of removing the precipitation of the protein produced by performing isoelectric point precipitation, and drying the concentrate obtained by subjecting the supernatant to microfiltration membrane treatment (Japanese Patent No. 3103218) etc. are mentioned.

The milk ceramide used in the present invention contains 15 to 35% by weight of protein and 45 to 65% by weight of lipid in the dried product, and the content of milk-derived phospholipid in the lipid may be 15% by weight or more in the dried product .

In the present invention, the method for producing the fermented horse placenta extract is not particularly limited, but for example, (1) primary fermentation at 25-35 ° C. for 1 to 5 days by adding water to the horse placenta and adding the selected fermented strain. making; (2) adding an enzyme to the first fermented product of the first step and performing secondary fermentation at 55 to 65° C. for 6 to 12 hours; (3) aging the secondary fermented product of the second step at 4 to 18° C. for 1 to 5 days; and (4) filtering the aged mixture of the third step to remove residues to obtain a fermented horse placenta extract, wherein the fermented strain is Aspergillus oryzae, Aspergillus Niger (Aspergillus niger), Aspergillus terreus (Aspergillus terreus), Aspergillus usami (Aspergillus usami), Penicillium chrysogenum (Penicillium chrysogenum), Penicillium notatum (Penicillium notatum) Mucor rouxii), and may be selected from the group consisting of combinations of two or more thereof, but is not limited thereto.

In the present invention, the "aloe vera (Aloe Vera, Barbados aloe)" is native to the Mediterranean region and Africa. The sap in the form of a gel contained in the leaves is known to be effective in treating burns and arthritis. In the first documented in the East, it was mentioned as presbytery in Gaebo Boncho (Gaebosangjeongboncho) compiled during the reign of King Taejo of the Song dynasty in the middle of the 10th century. It is a bushy perennial plant with short stems of 30-60cm in height and grows up to 100cm. From this stem, many long, hard leaves come out in the form of rosettes. The leaves are 15-40 cm long and 2-7 cm wide with white spots on a light green background, thick, fleshy, and juicy. It is wide at the bottom and narrows as it goes up, and the back is round and the front is slightly recessed. There are small, serrated, grayish spines on the margins. The tip of the leaf has some grooves and the tip is sharp. Small coffin-shaped flowers bloom in winter and spring. Flowers are bright yellow or orange in color and bloom downwards in raceme. It is easy to cultivate, resistant to pests, and has excellent oxygen production at night, making it a good indoor air purifying plant. It is suitable for indoor cultivation such as bedrooms, living rooms, and offices, and is often used as a medicinal plant. It is used for various purposes such as raw materials for cosmetics and pharmaceuticals, food, etc. It has been found to be effective in sterilization and neutralization of toxins, recovery from fatigue and neutralization of skin, antipyretic, constipation and ulcers. Vitamins A, C, D, E, B12, fatty acids, amino acids, selenium, calcium, aloin (aloin), aloesin (aloesin), etc. contains about 300 various components. The distribution area is very wide, such as India, North Africa, and Manila.

In the present invention, as the aloe vera, an aloe vera stock solution may be used, or an aloe vera extract may be used. Here, the "extract" refers to a solvent in which the active ingredient contained in the extracting material is transferred by contacting the solvent and the extracting material under specific conditions. They can all be included regardless of the type of ingredient. For example, extracts of components soluble in a solvent from a natural product using water or an organic solvent, and products obtained by extracting only a specific component such as a specific component of a natural product, such as oil, may be included.

In the present invention, the at least one selected from the group consisting of milk ceramide, fermented horse placenta extract, and aloe vera is used in an amount of 0.001 to 0.50% by weight, preferably 0.005 to 0.010% by weight, more preferably 0.01 to 0.05% by weight. It may be included, and when included in an amount of less than 0.001% by weight, a desired level of sufficient skin improvement and improvement effect cannot be obtained, and when included in an amount exceeding 0.50% by weight, the content of collagen peptides or other components is relatively reduced. There is a problem in that the absorption rate of collagen is lowered.

Mango Extract, Grapefruit Extract and Orange Extract

The food composition of the present invention further comprises at least one selected from the group consisting of mango extract, grapefruit extract and orange extract, so that even if a fish-derived collagen peptide is used as the collagen peptide, unpleasant smell and taste such as fishy smell can be masked. have.

In the present invention, to obtain the mango extract, grapefruit extract, or orange extract, the extraction method of mango, grapefruit or orange includes cold extraction, heat extraction, ultrasonic extraction, reflux cooling extraction, etc. all commonly used by those skilled in the art. An extraction method may be used.

In the present invention, a polar solvent or a non-polar solvent may be used as the solvent for the extraction. In the present invention, as the polar solvent, (i) water, (ii) alcohol (in an example of the present invention, methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, 1-pentanol, 2-butoxyethanol, ethylene glycol, propylene glycol, butylene glycol, glycerin), (iii) acetic acid, (iv) DMFO (dimethyl-formamide), and (v) DMSO (dimethyl sulfoxide) may be selected from, but limited thereto it is not going to be In the present invention, the non-polar solvent includes methylene chloride, acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, Diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, Diethyl sulfide, chloroform, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride, THF, caprylic/capric triglyceride, dimethicone, mineral oil, cyclomethicone, octyldodecanol, It may be selected from, but not limited to, cetylethylhexanoate, trirethylhexanoin, isopropylmyristate and vegetable oils. Here, the vegetable oil refers to an oil extracted by a method such as pressing the seeds or flesh of a plant, and olive oil, sunflower seed oil, jojoba oil, palm oil, avocado oil, etc. may be used, but is not limited thereto. .

In the present invention, at least one selected from the group consisting of the mango extract, grapefruit extract and orange extract may be included in an amount of 5 to 20% by weight, preferably 10 to 15% by weight, and included in an amount of less than 5% by weight. In this case, the inhibitory effect of the fish-derived collagen peptide on the unpleasant odor is insignificant, and when it is included in an amount of more than 20% by weight, it is excessive and rather deteriorates the flavor of the food composition, and the content of other functional ingredients including collagen peptide is relatively As a result, it is not possible to obtain a sufficient skin improvement and improvement effect at the desired level.

other additives

The food composition of the present invention may further comprise 5 to 20% by weight of specification honey, preferably 10 to 15% by weight, in order to improve flavor.

In addition, the food composition of the present invention may further include a sweetener in an amount of 1 to 10% by weight, preferably 3 to 8% by weight. In the present invention, the sweetener is sugar, glucose, maltose, oligosaccharide, dextrin, alpha cyclodextrin, beta cyclodextrin, gamma cyclodextrin, methyl beta cyclodextrin, hydroxypropyl beta cyclodextrin, cluster dextrin, ovarian Hwaseong dextrin, hydrogenated indigestible dextrin, invert sugar, fructose, lactose, galactose, starch syrup, sorbitol, maltitol, xylitol, erythritol, hydrogenated starch syrup, mannitol, and may be one or more components selected from the group consisting of trehalose, but is limited thereto it is not

The food composition of the present invention can mask the unpleasant taste by further including the steviol glycoside in an amount of 0.01 to 5% by weight, preferably 0.1 to 3% by weight. Specific examples of the steviol glycoside in the present invention include stenbitenlite (stevioside 98% or more) produced by Daepyeong Co., Ltd., stenbitenrich (enzyme-treated stevia 100%), stevia extract REB-A73% (Rebaudio) Side A 73% or more) and Revaten 97% (Rebaudioside A 97% or more), etc., but are not limited thereto.

The food composition of the present invention may further include malt extract in an amount of 0.01 to 5% by weight, preferably 0.1 to 3% by weight.

The food composition of the present invention further comprises at least one selected from the group consisting of konjac, carrageenan, xanthan gum, gellan gum and locust bean gum in an amount of 0.1 to 10% by weight, preferably 0.1 to 5% by weight, thereby forming a jelly formulation. can be formed However, in the case of manufacturing in a formulation other than that, the above components may be omitted.

The food composition of the present invention may contain a solvent as the balance, and the solvent may be water, and the water does not need to be particularly limited when suitable for food production, but for example, groundwater, purified water, distilled water, deionized water, etc. can be used

food composition

In the present invention, the 'food composition' includes, in addition to the active ingredients listed above, food raw materials that can be used as foods described in the standards and standards ('Food Code') of foods commonly used in food manufacturing, and food additives described in the Food Additives Code. may include

In the present invention, the food composition is not particularly limited, but may be, for example, a powder, granules, tablets, capsules, pills, extracts, jelly formulations, tea bag formulations or beverage formulations.

In addition, the above food composition can be added to general food to give skin improvement functionality, and the food that can be added does not need to be particularly limited, but for example, standards and standards for food according to Article 7 of the Food Sanitation Act (' Confectionery, bread or rice cakes, cocoa processed products or chocolates, processed meat or eggs, processed fish meat products, tofu or jelly products, noodles, tea, coffee, beverages, special purpose foods, soy sauce, seasoned foods, dressings It can be added to vegetables, kimchi, salted fish, pickled foods, stewed foods, alcoholic beverages, raisins, and other foods. In addition, it can be added to dairy products, processed meat products, packaged meat, and processed eggs exemplified in the processing standards and ingredient specifications of livestock products according to Article 4 of the Livestock Products Sanitation Control Act ('Livestock Products Code').

The food composition of the present invention can be used as "health functional food that helps improve skin". Here, the 'health functional food' refers to a food manufactured (including processing) according to legal standards using raw materials or ingredients useful for the human body (Article 3, No. 1 of the Health Functional Foods Act). The term 'health functional food' may differ in terms or scope of each country, but 'Dietary Supplement' in the US, 'Food Supplement in Europe', 'Health functional food' or 'Health functional food' in Japan It may correspond to 'Food for Special Health Use (FoSHU)' and 'health food' in China.

In the present invention, the food composition or health functional food may additionally contain food additives, and the suitability as a food additive is determined according to the general rules and general test methods of the 'Food Additives Codex' unless otherwise specified. and standards.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are only illustrative of the present invention, and the content of the present invention is not limited by the following examples.

Example

[Preparation Example 1] Preparation of collagen peptides

In order to prepare a food composition for skin improvement as a preparation example below, a collagen peptide corresponding to an active ingredient was prepared. In this case, as the collagen peptide, a collagen peptide derived from fish, having an average molecular weight of 439 Da and a GPH content of 3.6 wt%, was prepared.

[Preparation Examples 1 to 5] Preparation of a food composition for skin improvement

In purified water, the collagen peptide obtained in Preparation Example 1 and other ingredients shown in Table 1 were mixed to prepare a skin improvement food composition.

division Composition (wt%) Preparation Example 1 Preparation 2 Preparation 3 Preparation 4 Preparation 5 Preparation 6 collagen peptide 20 20 20 20 20 20 fruit sugar 5 5 5 5 5 5 Grapefruit Concentrate - - - - - 3 Mango Concentrate - - - - - 9 hyaluronic acid 0.01 0.01 0.01 0.01 0.01 0.01 vitamin C 0.01 0.01 0.01 0.01 0.01 0.01 milk ceramide - 0.01 0.01 0.01 0.01 0.01 Fermented horse placenta extract - 0.01 0.01 0.01 0.01 0.01 N-acetylglucosamine 0.01 0.01 - 0.01 0.01 0.01 Dry yeast containing 2.5% by weight of glucotathione 0.01 0.01 0.01 - 0.01 0.01 elastin 0.01 0.01 0.01 0.01 0.01 0.01 aloe vera - 0.01 0.01 0.01 0.01 0.01 biotin 0.01 0.01 0.01 0.01 - 0.01 propolis 0.001 0.001 0.001 0.001 0.001 0.001 Natural Orange Flavor (AI-F-0213) One One One One One One specification honey 10 10 10 10 10 10 konjac 0.1 0.1 0.1 0.1 0.1 0.1 Locust Bean Gum (Food Gel A) 1.8 1.8 1.8 1.8 1.8 1.8 Carrageenan (AKW Carrageenan) 0.2 0.2 0.2 0.2 0.2 0.2 Steviol glycoside (Levaten 97) 0.3 0.3 0.3 0.3 0.3 0.3 malt extract 0.6 0.6 0.6 0.6 0.6 0.6 anhydrous citric acid 1.7 1.7 1.7 1.7 1.7 1.7 Purified water balance balance balance balance balance balance

[Experimental Example 1] Evaluation of skin improvement effect

1. Mouse Experiment Preparation

After purchasing 6-week-old, 25-30 g of specific pathogen free (SPF) female hairless mice, the groups were divided into 8 groups of 5 mice each. For the feed, mouse-only feed (Purina, St. Louis, MO, USA) was freely fed, and for the negative water, UV-sterilized tap water was freely fed. The temperature was 23±2℃, and the humidity was 50±10%, and the animals were bred in a light/dark cycle of 12 hours. For the following experiments, first, the experimental animals were divided into 5 groups, and 8 mice were randomly assigned to each group. For each mouse group, the same treatment as in Table 2 was performed. In detail, the first group was bred without any treatment as a control group, and the remaining groups were fed a feed in which the compositions of Preparation Examples 1 to 6 were mixed in an amount of approximately 1.5% (w/w), but a total of 6 UV was treated with 2MED 3 times a week for a week, and the efficacy of each composition was observed.

division process 1st group unprocessed 2nd group UV irradiation 3rd group UV irradiation + Preparation Example 1 composition feed 4th group UV irradiation + Preparation Example 2 composition feed 5th group UV irradiation + Preparation 3 composition feed 6th group UV irradiation + Preparation Example 4 composition feed 7th group UV irradiation + Preparation Example 5 composition feed 8th group UV irradiation + Preparation 6 composition feed

2. Evaluation of skin elasticity improvement effect

In order to evaluate the skin elasticity improvement effect, after the end of the experiment, mouse skin tissue was taken and skin elasticity was measured using a Cutometer SEM575 (Courage & Khazaka), and the results are shown in Table 3 below.

division Ur/Ue 1st group 0.8277 2nd group 0.5941 3rd group 0.7236 4th group 0.7139 5th group 0.6693 6th group 0.6579 7th group 0.6854 8th group 0.8637

As shown in Table 3, the skin elasticity was decreased when UV irradiation compared to the untreated group, but skin elasticity was increased when the compositions of Preparation Examples 1 to 6 were fed. In particular, when the composition of Preparation Example 6 was fed, it was confirmed that the skin elasticity was superior to that of not irradiated with UV.

3. Evaluation of skin moisturizing effect

The amount of transdermal water loss from the start date to the end date of the experiment was measured using a Vapometer (Delfin Technologies Ltd.), and the results are shown in Table 4 below. The measurement environmental conditions were a relative humidity of 50±5% and a temperature of 23.5±0.5°C.

division Transdermal water loss (g/m ² /h) 1st group 11 2nd group 32 3rd group 29 4th group 21 5th group 24 6th group 22 7th group 15 8th group 10

As shown in Table 4, the amount of transdermal moisture loss during UV irradiation was significantly increased compared to the untreated group, and when the compositions of Preparation Examples 1 to 6 were fed, the degree of transdermal moisture loss was improved, and in particular, the composition of Preparation Example 6 It was confirmed that in the case of feeding , it was possible to reduce the transdermal water loss to a level equal to or higher than that in the case of not irradiating UV.

[Experimental Example 2] Sensory evaluation

1. Satisfaction evaluation of skin improvement effect

After having 50 panelists take the composition of Preparation Examples 1 to 6 for 3 weeks, a satisfaction survey was conducted for skin improvement (comprehensive evaluation of skin elasticity, wrinkle improvement, moisturizing, and whitening), and skin improvement was 1 point less ~ They were asked to respond with 5 points a lot. In this case, the higher the score, the higher the degree of improvement. The results are shown in Table 5 below by averaging the scores evaluated by the 50 panelists.

division Satisfaction evaluation of skin improvement effect Preparation Example 1 3.8 Preparation 2 3.8 Preparation 3 3.2 Preparation 4 4.0 Preparation 5 3.6 Preparation 6 4.6

As shown in Table 5, all of the compositions of Preparation Examples 1 to 6 showed excellent evaluation scores in the sensory satisfaction evaluation for skin improvement effect, and in particular, the composition of Preparation Example 6 had a very high satisfaction score of 4.6. .

2. Evaluation of palatability for the composition

Fifty panelists were asked to evaluate the color, taste, flavor and overall preference of the compositions of Preparation Examples 1 to 6. For evaluation, responses were made on a scale of 1 point less to 5 points more, and a higher score meant a higher degree of preference. The results are shown in Table 6 below by averaging the scores evaluated by the 50 panelists.

division color taste incense symbol Preparation Example 1 4.0 2.6 2.4 3.2 Preparation 2 3.6 2.8 2.2 3.0 Preparation 3 4.1 3.4 3.2 3.6 Preparation 4 4.2 3.1 3.3 3.6 Preparation 5 4.4 4.2 4.0 4.2 Preparation 6 4.6 4.2 4.4 4.4

As shown in Table 6, all of the compositions of Preparation Examples 1 to 6 showed generally excellent evaluation scores in color, taste, flavor and preference, and in particular, the composition of Preparation Example 6 including grapefruit extract and mango extract was taste and It can be seen that the fragrance evaluation also obtained a very high score.

As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, these specific techniques are only preferred embodiments, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (16)

Contains collagen peptide, vitamin C, hyaluronic acid and elastin as active ingredients,
The collagen peptide has a glycine-proline-hydroxyproline (glycine-proline-hydroxyproline, GPH) content of 3.2% by weight or more,
The collagen peptide is 15 to 25% by weight; Vitamin C is 0.001 to 0.50% by weight; hyaluronic acid is 0.001 to 0.50% by weight; and elastin is included in an amount of 0.001 to 0.50% by weight,
Yeast, propolis, N-acetylglucosamine and biotin in an amount of 0.001 to 0.50% by weight,
Milk ceramide, fermented horse placenta extract and aloe vera in an amount of 0.001 to 0.50% by weight,
Mango extract and grapefruit extract in an amount of 5 to 20% by weight,
a solvent as the balance,
The collagen peptide is extracted from fish and has a molecular weight of 200 to 500 Da, a food composition for skin improvement. delete delete The method of claim 1,
The collagen peptide is contained in an amount of 6,000 mg or more, a food composition for skin improvement. delete delete delete The method of claim 1,
The yeast comprises 1.5% by weight or more of glutathione, a food composition for skin improvement. delete delete delete delete delete delete delete delete