KR20090103823A - Anti-aging agent external preparation for skin, and food and drink - Google Patents

Abstract

PURPOSE: An anti-aging agent, an external skin preparation and a food product are provided effectively prevent and treat skin aging such as elasticity reduction, roughness, and wrinkle. CONSTITUTION: An anti-aging agent contains extract of fermentation wormwood, extract of fermentation bagasse, extract of fermentation Psidium guajava, extract of fermentation silver silk-tree, and extract of fermentation Curcuma longa. The anti-aging agent has one or more functions of promoting skin fibroblast proliferation and tranglutaminase-1 generation, suppressing elastase activity and MMP-1 activity, and recovering skin damage by UVB.

Description

Anti-aging agent external preparation for skin, and food and drink}

The present invention relates to an anti-aging agent containing a plant fermentation extract, an external preparation for skin and food and drink using the anti-aging agent.

The epidermis and dermis of the skin is composed of epidermal cells, skin fibroblasts, and extracellular matrices such as collagen and elastin that support the skin structure outside of these cells. In younger skin, the interaction of these skin tissues maintains homeostasis, thereby ensuring moisture retention, flexibility, and elasticity, and the skin remains apparently supple and shiny.

By the way, there are influences of external factors such as irradiation of ultraviolet rays (UV-A, UV-B), remarkable drying of air, excessive skin cleaning, contact with hydrogen peroxide, or, for example, collagen or elastin. Decreases the amount of extracellular matrix produced, and causes elasticity decrease due to crosslinking. As a result, the skin loses its moisturizing function and elasticity, and keratin starts abnormal peeling, so the skin loses its softness and luster, and exhibits aging symptoms such as roughness and wrinkles.

As described above, it is known that reductions or denaturation of dermal matrix components such as collagen and elastin are involved in changes associated with aging of the skin, that is, wrinkles, dullness, loss of fine skin texture and decrease in elasticity.

Among collagen, type I collagen is the most collagen contained in the body, and it is known that it is contained in the dermis of skin and plays the role which produces the firmness of skin.

Elastase is an elastin hydrolase present in the dermis of the skin. Elastase may be overexpressed by UV exposure or aging. When elastin is denatured and destroyed by elastase, It is thought that elasticity falls.

In addition, a group of protease enzymes called matrix metalloproteases (hereinafter sometimes referred to as "MMPs") can be cited as factors that induce reduction or denaturation of dermal matrix components in recent years.

The MMPs are characterized by (1) collagenase groups (MMP-1, MMP-8 and MMP-13), (2) gelatinase groups (MMP-2 and MMP-9), (3) stromelysin group (MMP-3 and MMP-10), (4) membrane-bound matrix metalloprotease group (MMP-14, MMP-15, MMP-16, and MMP-17), (5) other (MMP-7, MMP-11, and MMP-12) in five groups (see Japanese Patent Laid-Open No. 2000-344672).

Among the MMPs, MMP-1 and MMP-14 are known as enzymes that decompose type I collagen, type II collagen and type III collagen, which are the main constituents of the dermal matrix of the skin. In addition, the expression is greatly increased by irradiation of ultraviolet rays, which is one of the causes of the reduction or denaturation of collagen caused by ultraviolet rays, and is considered to be a large factor such as wrinkle formation of the skin.

In addition, as one of the causes of skin aging, for example, the secretion of estrogen, which is a type of female hormone, is reduced. Estrogen is deeply involved in maintaining the health of adult women, and its lack of secretion leads to various medical diseases, and it is known to cause undesirable skin changes such as skin hypersensitivity, decreased elasticity, and reduced moisture. .

In addition, the stratum corneum is formed of keratinocytes formed by terminal differentiation of epidermal keratinocytes and intercellular lipids, which are intercellular. Intercellular lipids containing ceramide as a main component are responsible for the stratum corneum barrier function by forming a lamellar structure. Keratin cells, on the other hand, are composed of keratin fibers as a major component and in a hydrophobic and tough cell membrane-like structure called cornified envelope (hereinafter, abbreviated as "CE"), which is a lining protein of membrane. Covered. CE forms a plurality of CE precursor proteins such as involucrin and loricrin, which are produced intracellularly by differentiation of epidermal keratinocytes, by cross-linking and insolubilizing by enzyme transglutaminase-1. This CE is closely involved in the barrier function of the skin. In addition, ceramide and the like are covalently bonded to a part thereof to take a hydrophobic structure, thereby providing a foundation for the lamellar structure of intercellular lipids, thereby forming a foundation of the stratum corneum barrier function and skin moisture retention function.

However, if an abnormality occurs in the turnover speed due to the effects of drying or ultraviolet rays (UV-A, UV-B), for example, the so-called parakeratosis, in which the lamellar structure is disturbed or the CE is incompletely formed. Induced, abnormality in the structure of keratinocytes and intercellular lipids, the water retention function and barrier function of the stratum corneum is reduced. It is thought that this leads to aging symptoms of skin such as rough skin and dry skin. In the case of patients with psoriasis or atopic dermatitis, immature CE is observed at high frequency in the lowered part of the skin where barrier function is reduced, and it is considered that it is very important for the barrier function of the skin. (See Experimental Dermatology 12: 591-601 (2003)).

From these facts, skin fibroblast proliferation, transglutaminase-1 production, elastase activity inhibition, MMP-1 activity inhibition, estrogen-like action, type I collagen production promotion, epidermal keratinization It is thought that anti-aging agent having each action such as promoting cell proliferation and restoring from UV-B damage can effectively prevent or improve skin aging symptoms such as decreased elasticity, roughness and wrinkles of the skin. .

However, to date, it is an easy-to-use, inexpensive, high-safety natural product, and does not adversely affect the quality of the added object in terms of taste, smell, and usability, and thus is widely used for external skin preparations and food and drink. Has not been provided yet, so the prompt provision is strongly required.

This invention solves the problem in the said prior art, and makes it a subject to achieve the following objectives. That is, the present invention has excellent anti-aging action (promoting skin fibroblast proliferation, promoting transglutaminase-1 production, inhibiting elastase activity, inhibiting MMP-1 activity, estrogen-like action, type I collagen production) It is aimed to provide an anti-aging agent having high promoting action, promoting epidermal keratinocyte proliferation, restoring from UV-B damage, etc., and a highly safe anti-aging agent, an external preparation for skin and food and drink using the anti-aging agent.

In order to solve the above problems, the present inventors have made intensive studies, and further extract each plant fermentation product obtained by fermenting each plant of mugwort, bagasse, guava, moth, silver bark, turmeric, and woldo, respectively. The obtained plant fermentation extracts promote skin fibroblast proliferation, transglutaminase-1 production, elastase activity inhibition, MMP-1 activity inhibition, estrogen-like activity, type I collagen production promotion, The present invention has been found to have excellent anti-aging action based on at least one of epidermal keratinocyte proliferation promoting action and recovery from UV-B damage.

This invention is based on the said knowledge of this inventor, etc., As a means for solving the said subject, it is as follows. In other words,

<1> contains at least one selected from extracts of fermented mugwort, extracts of fermented bagus, extracts of fermented guava, extracts of fermented moth, extract of fermented silverwood, extract of fermented turmeric, and extract of fermented moonshine It is an anti-aging agent characterized in that.

<2> Skin fibroblast proliferation, transglutaminase-1 production promotion, elastase activity inhibition, MMP-1 activity inhibition, estrogen-like action, type I collagen production promotion, epidermal keratinocyte proliferation It is an anti-aging agent as described in said <1> which has at least any of a promoting action and a recovery action from UV-B damage.

<3> A skin external preparation containing the anti-aging agent according to any one of <1> to <2>.

<4> Food and drink characterized by containing the anti-aging agent in any one of said <1> to <2>.

ADVANTAGE OF THE INVENTION According to this invention, it is possible to solve the problem in the prior art and to achieve the above object, and to have excellent anti-aging action (promoting skin fibroblast proliferation, transglutaminase-1 production promoting action, elastase activity). Inhibition, MMP-1 activity inhibition, estrogen-like action, type I collagen production promotion, epidermal keratinocyte proliferation, recovery from UV-B damage, etc.) The external preparation for skin and food and drink using an anti-aging agent can be provided.

The anti-aging agent, external preparation for skin and food and drink of the present invention can be used on a daily basis, and by the action of each of the above-mentioned plant fermentation extracts as active ingredients, it promotes skin fibroblast proliferation and transglutaminase-1 production. Based on at least one of elastase inhibitory activity, MMP-1 activity inhibitory, estrogen-like activity, type I collagen production promotion, epidermal keratinocyte proliferation, and recovery from UV-B damage Aging can be very effective. For this reason, according to the anti-aging agent, the external preparation for skin, and the food-drinks of this invention, it is anticipated that the prevention and improvement of skin aging symptoms, such as skin elasticity fall, roughness, and wrinkles, can be performed effectively.

In addition, although the anti-aging agent, the external preparation for skin, and the food-drinks of this invention are suitably applied to a human, as long as the effect is exhibited, animals other than humans (for example, a mouse, a rat, a hamster, a dog, a cat, Cattle, pigs, monkeys, etc.). In addition, the anti-aging agent, the external preparation for skin, and the food and drink of the present invention are made from natural plant-derived fermented extracts as active ingredients, and are also advantageous in terms of excellent safety.

(Anti-aging agent)

The anti-aging agent of the present invention is selected from the extract of fermented mugwort, the extract of fermented bagasse, the extract of fermented guava, the extract of fermented moth, the extract of fermented silverwood, the extract of fermented turmeric, and the extract of fermented woldo It may contain species or more, and may contain other components as necessary.

Here, the extract of the fermented mugwort extract, the extract of fermented bagus, the extract of fermented guava, the extract of fermented moth, the extract of fermented silverwood, the extract of fermented turmeric, and the extract of fermented moonshine, respectively, mugwort, bagasse, Extract obtained by additionally extracting a fermentation product (sometimes referred to as "plant fermentation product" in the present specification) obtained by fermenting each plant of guava, nirvana, herbarium, turmeric, and woldo (in this specification, The term "plant fermentation extract" may be referred to).

The anti-aging agent promotes skin fibroblast growth, transglutaminase-1 production, elastase activity inhibition, MMP-1 activity inhibition, estrogen-like action, type I collagen production promotion, epidermal keratinization It has an anti-aging action based on at least one of a cell proliferation promoting action and a recovery action from UV-B damage.

Substances exhibiting anti-aging activity in the extract of the fermented mugwort, the extract of fermented bagasse, the extract of fermented guava, the extract of fermented moth, the extract of fermented silverwood, the extract of fermented turmeric, and the extract of fermented moonshine Although it is unclear about the detail of the above, the fact that each said plant fermentation extract has such an excellent effect and is useful as an anti-aging agent is not known at all, and it is a new knowledge by this inventor.

The wormwood is a plant of the Asteraceae, the scientific name is Artemisia princeps . The wormwood is perennial, widely distributed throughout Japan, and can be easily obtained from these areas.

There is no restriction | limiting in particular as a site | part of the said mugwort used as a fermentation raw material, Although it can select suitably according to the objective, For example, a flower, a bud, a fruit, a peel, a seed, a seedling, a stem, a leaf, a branch, a branch leaf, a tree trunk, a bark , Roots, rhizomes, rhizomes, mixtures thereof, and the like, of which leaves and stems are preferred.

The bagasse is a squeezing dreg of sugarcane generated in the process of preparing sugar from sugarcane ( Saccharum officinarum ). For this reason, the said bagus can be easily obtained as a waste at the time of manufacturing sugar from sugar cane.

There is no restriction | limiting in particular as said bagus used as a fermentation raw material, According to the objective, it can select suitably, For example, a raw baggar, a dry baggar, etc. are mentioned, Among these, dry baggar is preferable.

The guava is a Platinum family, scientific name is Psidium guajava Linn. The guava is evergreen, widely distributed in tropical and subtropical regions such as Southeast Asia, South China, Hawaii, and can be easily obtained from these regions.

There is no restriction | limiting in particular as a site | part of the said guava used as a fermentation raw material, Although it can select suitably according to the objective, For example, a flower, a bud, a fruit, a peel, a seed, a seed, a stem, a leaf, a branch, a branch, a tree trunk, a bark , Roots, rhizomes, rhizomes, mixtures thereof, and the like, among which leaves are preferred.

The moth is a plant of the family Asteraceae, scientific name is Crepidiastrum lanceolatum Nakai. The above-mentioned moth is a perennial plant, which is widely vegetated and distributed in mountains and fields of Japan, such as Okinawa, and can be easily obtained from these areas.

There is no restriction | limiting in particular as a site | part of the said bark used as a fermentation raw material, Although it can select suitably according to the objective, For example, a flower, a bud, fruit, a peel, a seed, a seed, a stem, a leaf, a branch, a branch, a tree trunk, a bark , Roots, rhizomes, rhizomes, mixtures thereof, and the like, among which leaves are preferred.

The herbaceous tree is a legume plant, scientific name is Leucaena leucocephala . The herbaceous tree is a perennial plant, widely distributed in the tropics and subtropics, and is also cultivated in Okinawa in Japan, and can be easily obtained from these areas.

There is no restriction | limiting in particular as a site | part of the said silverwood tree used as a fermentation raw material, Although it can select suitably according to the objective, For example, a flower, a bud, fruit, a rind, a seed, a seed, a stem, a leaf, a branch, a branch, a tree trunk , Bark, root, rhizome, root bark, and mixtures thereof. Among these, leaves and stems are preferable.

The turmeric is a plant of the family Ginger, scientific name is Curcuma longa L. The turmeric is a perennial plant and is cultivated in the tropical regions of India, Southeast Asia, southern China, and even in Okinawa in Japan, and can be easily obtained from these regions.

There is no restriction | limiting in particular as a site | part of the said turmeric used as a fermentation raw material, Although it can select suitably according to the objective, For example, a flower, a bud, a fruit, a peel, a seed, a seedling, a stem, a leaf, a branch, a branch leaf, a tree trunk, a bark , Roots, rhizomes, rhizomes, mixtures thereof, and the like, among which rhizomes are preferred.

Woldo is a plant of the family Ginger, scientific name is Alpinia speciosa K. Schum. The moon is perennial, widely distributed from southern Kyushu to southern China to tropical Asia, and can be easily obtained from these regions.

There is no restriction | limiting in particular as said site | part of the said woldo used as a fermentation raw material, Although it can select suitably according to the objective, For example, a flower, a bud, a fruit, a peel, a seed, a seedling, a stem, a leaf, a branch, a branch leaf, a tree trunk, Bark, root, rhizome, root bark, mixtures thereof and the like, among which leaves are preferred.

Each plant is used as a fermentation raw material for obtaining each plant fermentation product. By fermenting each said plant by arbitrary methods, each plant fermentation product can be obtained. There is no restriction | limiting in particular as a fermentation method of each said plant, Although it can select suitably according to the objective, For example, Unexamined-Japanese-Patent No. 2004-267100 (mugwort), Unexamined-Japanese-Patent No. 4031637 (mugwort, guava, walnut) Japanese Patent No. 4067805 (Burgas), Japanese Patent Laid-Open No. 2006-347985 (Cursor), Japanese Patent No. 2596472 (Silverwood), Japanese Patent No. 2949411 (Golden) And the method described in Japanese Patent Application Laid-Open No. 2004-345986 (Purple), Japanese Patent Application Laid-Open No. 2007-99625 (Mondo), and the like can be suitably employed. Specifically, for example, by drying each of the above-mentioned plants in the state as it is, or in a state in which the plants are ground using a crushing machine or the like, they are subjected to fermentation treatment by microorganisms such as lactic acid bacteria, yeast, Bacillus subtilis and the like. Each plant fermentation product can be obtained.

Each plant fermentation product obtained as mentioned above is used as an extraction raw material for obtaining each plant fermentation extract. Each plant fermentation product may be, for example, dried and then used for solvent extraction. The said drying may be performed in the sun, for example, and may be performed using the dryer normally used. In addition, each said plant fermentation product may be used as an extraction raw material after carrying out sterilization treatment. The said sterilization process can be performed by well-known methods, such as heating, for example. In addition, each said plant fermentation product may be used as an extraction raw material after carrying out pretreatment, such as degreasing with a nonpolar solvent, such as hexane and benzene. By performing pretreatment, such as degreasing, the extraction process by the polar solvent of each said plant fermentation product can be performed efficiently.

Each plant fermentation extract can be easily obtained by subjecting the plant fermentation to an extraction process using a method generally used for extraction of plants. Moreover, you may use a commercial item as said each plant fermentation extract. In addition, each plant fermentation extract includes an extract of each plant fermentation, a diluent or concentrate of the extract, a dried product of the extract, or a crude or purified product thereof.

There is no restriction | limiting in particular as a solvent used for the said extraction, According to the objective, it can select suitably, For example, it is preferable to use water, a hydrophilic organic solvent, or these mixed solvents at room temperature or the temperature below the boiling point of a solvent. . The component which exhibits the anti-aging effect contained in each said plant fermentation product can be extracted easily by the extraction process which uses a polar solvent as an extraction solvent.

There is no restriction | limiting in particular as water which can be used as the said extraction solvent, According to the objective, it can select suitably, For example, pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, etc. And those subjected to various treatments are included. The treatment to water includes, for example, purification, heating, sterilization, filtration, ion exchange, adjustment of penetration pressure, buffering, and the like. Accordingly, water that can be used as the extraction solvent includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

There is no restriction | limiting in particular as a hydrophilic organic solvent which can be used as said extraction solvent, According to the objective, it can select suitably, For example, C1-C5 lower alcohols, such as methanol, ethanol, propyl alcohol, isopropyl alcohol; Lower aliphatic ketones such as acetone and methyl ethyl ketone; C2-C5 polyhydric alcohols, such as 1, 3- butylene glycol, propylene glycol, and glycerin, These mixed solvents of a hydrophilic organic solvent and water, etc. can also be used. When using a mixed solvent of the water and the hydrophilic organic solvent, 1 to 90 parts by mass with respect to 10 parts by mass of water for lower alcohols, and 1 to 40 parts by mass with respect to 10 parts by mass of water of lower aliphatic ketones. It is preferable to use one. In addition, in the case of a polyhydric alcohol, it is preferable to use what mixed 1-90 mass parts with respect to 10 mass parts of water.

In addition, when fermentation month is used as an extraction material, among these solvents, skin fibroblast proliferation promoting effect, transglutaminase-1 production promoting effect, MMP-1 activity inhibitory effect, recovery from UV-B damage, etc. It is preferable to use the mixed solvent which mixed 5-20 mass parts of lower alcohols with respect to 10 mass parts of water from the point which can raise the.

In extracting an extract having an anti-aging action from each of the above-mentioned plant fermentation products, it is not necessary to employ a special extraction method, and may be extracted using any extraction apparatus at room temperature or under reflux heating.

Specifically, each of the above-mentioned extraction raw materials is added to a treatment tank filled with an extraction solvent, and the mixture is left still for 30 minutes to 4 hours while occasionally stirring as necessary, eluting the soluble components, and then filtering and solids. The extract can be obtained by removing the residue, distilling and extracting the extraction solvent from the obtained extract. The extraction solvent amount is usually 5 to 15 times (mass ratio) of the extraction raw material. Extraction conditions are usually about 1 to 4 hours at 50 to 95 ° C when water is used as the extraction solvent. In addition, when the mixed solvent of water and ethanol is used as an extraction solvent, it is about 30 minutes-about 4 hours at 40-95 degreeC normally. The extract obtained by extraction with a solvent can be used as it is as an active ingredient of the anti-aging agent of the present invention as long as the extraction solvent has high safety.

The extracts of the above-mentioned plant fermentation products obtained by extraction may be diluted, concentrated, dried or purified according to a conventional method in order to obtain a diluted or concentrated liquid of the extract, a dried product of the extract, or a crude or purified product thereof. You may perform a process. Moreover, although the extract liquid of each said plant fermentation obtained can be used as an active ingredient of an anti-aging agent as it is, it is easy to use what was used as the concentrated liquid or its dried product. In obtaining the dried material of an extract, a conventional method can be used, and in order to improve hygroscopicity, you may add carriers, such as dextrin and cyclodextrin. In addition, the above-mentioned plant fermented product, which is an extract raw material, may have a peculiar smell and taste. Therefore, the extract of each plant fermented product may be decolorized, deodorized, etc. in a range that does not cause a decrease in physiological activity. Although refinement | purification to be made is possible, for example, when adding to skin cosmetics, since it is not used in large quantities, even unrefined raw material does not interfere practically. In addition, the purification can be performed specifically by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment or the like.

The extract of each plant fermentation product (plant fermentation extract) obtained as described above is effective for promoting skin fibroblast proliferation, promoting transglutaminase-1 production, inhibiting elastase activity, inhibiting MMP-1 activity, estrogen It has at least one of similar action, type I collagen production promoting action, epidermal keratinocyte proliferation promoting action, and recovery from UV-B damage, and is suitable as an active ingredient of the anti-aging agent of the present invention based on these actions. Is available. In addition, the plant fermentation extract is based on the above-described action, the skin fibroblast proliferation promoter, transglutaminase-1 production promoter, elastase activity inhibitor, MMP-1 activity inhibitor, estrogen-like agent, type I collagen production It can be suitably used also as an accelerator, an epidermal keratinocyte proliferation promoter, and a recovery agent from UV-B damage.

There is no restriction | limiting in particular as content of the said plant fermentation extract in the said anti-aging agent, According to the objective, it can select suitably, Moreover, the said anti-aging agent may be said each plant fermentation extract itself.

Moreover, any 1 type of said each plant fermentation extract may be contained in the said anti-aging agent, and 2 or more types may be contained. There is no restriction | limiting in particular also as each content ratio in the case where 2 or more types of plant fermentation extracts are contained in the said anti-aging agent, According to the objective, it can select suitably.

The other components other than the above-mentioned plant fermentation extracts which may be included in the anti-aging agent are not particularly limited as long as they are within the range that does not impair the effects of the present invention, and may be appropriately selected according to the purpose. And a physiological saline solution for diluting the plant fermentation extract to a desired concentration. Moreover, there is no restriction | limiting in particular also in content of the said other component in the said anti-aging agent, According to the objective, it can select suitably.

In addition, the said anti-aging agent can be formulated as needed, and can be made into arbitrary formulations, such as powder form, a granule form, and a tablet form.

The anti-aging agent of the present invention promotes skin fibroblast proliferation, transglutaminase-1 production, elastase activity inhibition, MMP-1 activity inhibition, estrogen-like action, type I collagen production promotion, In addition to having excellent anti-aging action based on at least one of epidermal keratinocyte proliferation promoting action and recovery from UV-B damage, and excellent in safety, the external preparation for skin of the present invention described below, for example, It is suitable for use as food and drink.

(Skin external preparation)

The external preparation for skin of the present invention contains the anti-aging agent of the present invention described above, and may further contain other components as necessary.

Here, the external skin preparation means various drugs applied to the skin, and the distinction is not particularly limited, and includes, for example, skin cosmetics, quasi-drugs, and medicines widely.

The said external skin agent may be mix | blended with each skin external preparation, so that the said plant fermentation extract may not interfere with the activity, and the external skin preparation which has said each plant fermentation extract as a main component may be sufficient as it. In addition, the said skin external preparation may be said each plant fermentation extract itself.

There is no restriction | limiting in particular as said skin external preparation, According to the objective, it can select suitably, For example, ointment, cream, emulsion, beauty liquid, lotion, pack, foundation, lip cream, bath agent, hair tonic, hair lotion, soap, body shampoo, etc. Can be mentioned.

There is no restriction | limiting in particular as said other components, According to the objective, it can select suitably, The components normally used in manufacture of a skin external preparation, for example, astringent, antiseptic, antibacterial agent, whitening agent, ultraviolet absorber, moisturizer, cell activator, anti-aging Agents, anti-inflammatory and anti-allergic agents, antioxidants and active oxygen scavengers, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, and fragrances.

There is no restriction | limiting in particular as content of the said anti-aging agent in the said external skin agent, Although it can select suitably according to the kind of external skin agent etc., For example, 0.0001-10 mass% is preferable as the quantity of each said plant fermentation extract, 0.001-5 Mass% is more preferable.

(Beverage food)

The food-drinks of this invention contain the anti-aging agent of this invention mentioned above, and also contain other components as needed.

Here, the food and drink means that there is little risk of harm to human health and is ingested by oral or digestive tract administration in normal social life, and is limited to the division of food, medicine, quasi-drug, etc. in administrative division. It does not mean, for example, it means to include a general food, health food, health functional food, quasi-drugs, pharmaceuticals and the like ingested orally widely.

The food or drink may be formulated with any food or drink so as not to interfere with the activity of the respective plant fermentation extracts, or may be a dietary supplement containing the respective plant fermentation extracts as a main component. In addition, the said food-drinks may be said each plant fermentation extract itself.

There is no restriction | limiting in particular as said food-drinks, According to the objective, it can select suitably, For example, drinks, such as a soft drink, a carbonated drink, a nutritional drink, an fruit drink, a lactic acid drink; Frozen desserts such as ice cream, ice sherbet, and ice water; Noodles such as soba (buckwheat noodles), kara noodles, vermicelli, dumplings, steamed dumplings, medium noodles, and instant noodles; Confectionery such as candy, candy, gum, chocolate, sweets, snacks, biscuits, jelly, jam, cream, baked sweets and bread; Aquatic products, such as crab, salmon, clam, tuna, sardine, shrimp, bonito, mackerel, whale, oyster, saury, squid, shellfish, scallops, abalone, sea urchin, salmon roe, rice cake clam; Fish and livestock processed foods such as fish paste, ham and sausage; Dairy products such as processed milk and fermented milk; Fat and oil processed foods such as salad oil, fried oil, margarine, mayonnaise, shortening, whipped cream and dressing; Seasonings such as sauces and seasonings; Finely chopped curry, stew, chicken egg rice bowl, rice porridge, vegetables or seafood and seasoned with miso or soy sauce Retort pouch foods such as tang, mapo tofu, beef bowl, meat sauce, egg soup, omelet rice, dumplings, steamed dumplings, hamburg, meatballs; Health foods and nutritional supplements in various forms; Medicines such as tablets, capsules, drinks, troches, quasi drugs, and the like.

There is no restriction | limiting in particular as said other components, According to the objective, it can select suitably, For example, the auxiliary raw material or additives normally used in manufacturing food-drinks are mentioned.

There is no restriction | limiting in particular as said auxiliary raw material or additive, According to the objective, it can select suitably, For example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubus side, corn syrup, lactose, citric acid, tartaric acid, malic acid, Succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, gum arabic, carrageenan, casein, gelatin , Pectin, agar, vitamin B, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, flavorings, preservatives and the like.

The content of the anti-aging agent in the food and drink is different depending on the type of food and drink to be targeted and cannot be regulated uniformly. For example, the anti-aging agent is formulated into any food and drink within a range that does not impair the original taste of the food and drink. When it aims at that, 0.001 mass%-50 mass% are preferable, and, as for the quantity of each said plant fermentation extract which is an active ingredient, 0.01 mass%-20 mass% are more preferable. In addition, for example, in the case of manufacturing a dietary supplement such as granules, tablets, capsules, etc., each of which has the respective plant fermentation extracts as a main component, it is 0.01% by mass as the amount of each plant fermentation extract which is an active ingredient. 100 mass% is preferable and 5 mass%-100 mass% are more preferable.

EXAMPLE

Hereinafter, although the Example of this invention is described, this invention is not limited to these Examples at all.

(Manufacture example 1)

Production of Water Extracts of Each Plant Fermentation

As a plant fermentation product to be extracted, fermented mugwort, fermented bagasse, fermented guava, fermented moth, fermented herb, fermented turmeric, and fermented Woldo (all are manufactured by Ryukyu Bio-Resource Kaihatsu). . 100 g of each plant fermented product was added to 1,000 ml of water, and maintained at 90 ° C. for 2 hours with gentle stirring, followed by filtration. The filtrate was concentrated under reduced pressure at 40 ° C. and further dried with a vacuum dryer to obtain a powdery extract. The yield of the obtained extract (plant fermentation extract) is shown in Table 1.

(Manufacture example 2)

Preparation of 50% by Mass Ethanol Extract of Each Plant Ferment

As a plant fermentation product to be extracted, fermented mugwort, fermented bagasse, fermented guava, fermented moth, fermented herb, fermented turmeric, and fermented Woldo (all are manufactured by Ryukyu Bio-Resource Kaihatsu). . 100 g of each plant fermented product was added to 1,000 ml of 50 mass% ethanol (mass ratio of water and ethanol 1: 1), the mixture was kept at 90 ° C. for 2 hours with gentle stirring, and then filtered. The filtrate was concentrated under reduced pressure at 40 ° C. and further dried with a vacuum dryer to obtain a powdery extract. The yield of the obtained extract (plant fermentation extract) is shown in Table 1.

(Manufacture example 3)

Preparation of 80 Mass% Ethanol Extract of Each Plant Fermentation

As a plant fermentation product to be extracted, fermented mugwort, fermented bagasse, fermented guava, fermented moth, fermented herb, fermented turmeric, and fermented Woldo (all are manufactured by Ryukyu Bio-Resource Kaihatsu). . 100 g of each plant fermented product was added to 1,000 ml of 80 mass% ethanol (mass ratio of water and ethanol 1: 4), and the mixture was kept at 90 ° C. for 2 hours with gentle stirring, followed by filtration. The filtrate was concentrated under reduced pressure at 40 ° C. and further dried with a vacuum dryer to obtain a powdery extract. The yield of the obtained extract (plant fermentation extract) is shown in Table 1.

Example 1 Skin Fibroblast Proliferation Promotion Test

Using each plant fermented extract as a test sample, the skin fibroblast proliferation promoting effect was tested by the following test method.

Human normal dermal fibroblasts (NB1RGB) were cultured using α-MEM containing 10% FBS, and then cells were recovered by trypsinization. The recovered cells were diluted with α-MEM containing 5% FBS at a concentration of 7.0 × 10 ⁴ cells / mL, and then seeded in a well of a 96 well plate at 100 μL per well and incubated overnight. After the completion of the culture, 100 μL of the test sample dissolved in α-MEM containing 5% FBS was added to each well and incubated for 3 days. Dermal fibroblast proliferation was measured using the MTT assay. After incubation, 100 μL of the medium was removed from each well, and 20 μL of MTT dissolved in PBS (−) at a final concentration of 5 mg / mL was added to each well. After incubation for 4.5 hours, 100 μL of 0.01 mol / L hydrochloric acid solution in which 10% SDS was dissolved was added to each well, followed by incubation overnight, and the absorbance at wavelength 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between them was defined as the amount of blue formazan produced. In addition, a blank test was carried out in the same manner and corrected.

The calculation method of the skin fibroblast proliferation promoting action is as follows. The results are shown in Tables 2-7.

Skin fibroblast growth rate (%) = (St-Sb) / (Ct-Cb) × 100

St: Absorbance in Cells Added Test Samples

Sb: absorbance of blank test with test sample

Ct: absorbance in cells without addition of test sample

Cb: absorbance of blank test without addition of test sample

Example 2: Transglutaminase-1 Production Promoting Test

Using each plant fermented extract as a test sample, the transglutaminase-1 production promoting action was tested by the following test method.

Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using human normal neonatal epidermal keratinocyte medium (KGM), and then cells were recovered by trypsinization. The collected cells were diluted with KGM so as to have a concentration of 1 × 10 ⁵ cells / mL, and then seeded in a 96 well plate at 100 μL per well, and cultured for 2 days. After the end of the culture, 100 μL of the test sample dissolved in KGM was added to each well, and cultured for 24 hours. After the end of the culture, the medium was removed, the cells were fixed to the plate, and the amount of transglutaminase-1 expressed on the cell surface was measured by ELISA using monoclonal antihuman transglutaminase-1 antibody.

The calculation method of the transglutaminase-1 production promoting action is as follows. The results are shown in Tables 8-12.

Transglutaminase-1 Production Promotion Rate (%) = A / B × 100

A: absorbance at wavelength 405 nm at the time of test sample addition

B: absorbance at a wavelength of 405 nm when no test sample was added (control)

Example 3: Elastase Activity Inhibition Test

Using each plant fermented extract as a test sample, the elastase activity inhibitory effect was tested by the following test method.

In a 96 well plate, 50 μL of the test sample prepared with 0.2 mol / L Tris-HCL buffer (pH 8.0) and 50 μL of the 20 μg / mL elastase type III solution were mixed. Thereafter, 100 µL of 0.4514 mg / mL N-SUCCINYL-ALA-ALA-ALA-p-NITROANILIDE prepared with the above buffer was added and reacted at 25 ° C for 15 minutes. After completion of the reaction, the absorbance at wavelength 415 nm was measured. A blank test was performed in the same manner to correct.

The calculation method of the elastase activity inhibitory effect is as follows. The results are shown in Tables 13-14.

% Inhibition of elastase activity = {1- (C-D) / (A-B)} × 100

A: Absorption at wavelength 415 nm with no sample added and enzyme addition

B: Absorbance at a wavelength of 415 nm with no test sample and no enzyme

C: Absorbance at wavelength 415 nm at the addition of test sample and addition of enzyme

D: Absorbance at wavelength 415 nm at the addition of test sample and no enzyme

Example 4: MMP-1 activity inhibition test

Each plant fermented extract was used as a test sample, and the inhibitory effect of MMP-1 activity was tested by the following test method.

As a test method, a partly modified Wunsch and Heidrich method was used. That is, 50 μL of the sample dissolved in 20 mmol / mL calcium chloride-containing 0.1 mol / L Tris-HCl buffer (pH 7.1) and 50 μL of MMP-1 (COLLAGENASE Type IV from Clostridium histolyticum (Sigma)) solution in a test tube with a lid , And 400 μL of Pz-peptide (Pz-Pro-Leu-Gly-Pro-D-Arg-OH (BACHEM Feinchemikalien AG)) solution were mixed and reacted at 37 ° C. for 30 minutes, followed by 25 mmol / L citric acid. The reaction was stopped by addition of 1 mL of solution. Thereafter, 5 mL of ethyl acetate was added thereto, followed by vigorous shaking. This was centrifuged (1,600 * g, 10 minutes), and the absorbance in wavelength 320nm of an ethyl acetate layer was measured. A blank test was performed in the same manner to correct.

The calculation method of MMP-1 activity inhibitory activity is as follows. The results are shown in Tables 15 to 19.

% Inhibition of MMP-1 activity = {1- (C-D) / (A-B)} × 100

A: Absorption at wavelength 320nm without addition of test sample and addition of enzyme

B: Absorbance at a wavelength of 320 nm with no test sample and no enzyme

C: absorbance at wavelength 320 nm at the addition of test sample and addition of enzyme

D: Absorbance at wavelength 320 nm at the addition of test sample and no enzyme

Example 5: Estrogen-like Action Test

Using each plant fermented extract as a test sample, the estrogen-like action was tested by the following test method.

Human breast cancer derived cells (MCF-7) were cultured using MEM containing 10% FBS, 1% NEAA, and 1 mmol / L sodium pyruvate, and then cells were recovered by trypsinization. The recovered cells were diluted to a concentration of 3.0 × 10 ⁴ cells / mL using phenol red-free MEM (T-MEM) containing 10% FBS, 1% NEAA, and 1 mmol / L sodium pyruvate treated with activated carbon. Thereafter, 450 μL per well was seeded in 48 well plates and cultured to settle the cells. After 6 hours (day 0), 50 μL of the test sample prepared at 10 times the final concentration by T-MEM was added to each well to continue the culture. On day 3, the medium was removed, and 0.5 mL of the test sample prepared in T-MEM at the final concentration was added to each well, and the culture was further continued. Estrogen-like action was measured using an MTT assay. After the end of the culture, the medium was removed, and 200 μL of MTT dissolved in a well concentration of 0.4 mg / mL in MEM containing 1% NEAA and 1 mmol / L sodium pyruvate was added to each well. After incubation for 2 hours, the blue formazan produced in the cells was extracted with 200 μL of 2-propanol. After extraction, the absorbance at wavelength 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between them was defined as the amount of blue formazan produced. 1 × 10 ⁻⁹ M estradiol was used as a positive control.

The calculation method of the estrogen-like action is as follows. The results are shown in Tables 20 to 24.

% Estrogen-like action = A / B × 100

A: absorbance at the time of addition of the test sample

B: Absorbance when No Sample Added

Example 6 Type I Collagen Production Promoting Test

Using each plant fermented extract as a test sample, the type I collagen production promoting action was tested by the following test method.

Human normal fibroblasts (NB1RGB) were incubated with Dulbecco's MEM containing 10% FBS, and then cells were harvested by trypsinization. The collected cells were diluted with the medium at a concentration of 1.6 × 10 ⁵ cells / mL, and then seeded in a 96 well microplate at 100 μL per well and incubated overnight. After the end of the culture, the medium was removed, and 150 μL of the test sample dissolved in Dulbecco's MEM containing 0.25% FBS was added to each well, and cultured for 3 days. After incubation, the amount of type I collagen in the medium of each well was measured by ELISA.

The calculation method of the type I collagen production promoting action is as follows. The results are shown in Tables 25 to 29.

Type I collagen production promotion rate (%) = A / B × 100

A: amount of collagen type I when the test sample is added

B: Type I collagen content when no test sample was added

Example 7: Epidermal keratinocyte proliferation promoting test

Each plant fermented extract was used as a test sample, and the epidermal keratinocyte proliferation promoting effect was tested by the following test method.

Normal human neonatal foreskin epidermal keratinocytes (NHEK) were cultured using proliferation medium (EpiLife-KG2) for long-term culture of normal human epidermal keratinocytes, and then cells were recovered by trypsin treatment. The recovered cells were diluted with EpiLife-KG2 at a concentration of 1.5 × 10 ⁴ cells / mL (for fermentation, fermented turmeric) or 2.0 × 10 ⁴ cells / mL (for fermentation month), followed by collagen-coated 96 100 μL per well was seeded into well plates and incubated overnight. After the end of the culture, 100 μL of the test sample dissolved in EpiLife-KG2 was added to each well and incubated for 3 days. Epidermal keratinocyte proliferation promoting activity was measured using the MTT assay. After the end of the culture, the medium was removed, and 100 μL of MTT dissolved in PBS (−) at a final concentration of 0.4 mg / mL was added to each well. After incubation for 2 hours, the blue formazan produced in the cells was extracted with 100 μL of 2-propanol. After extraction, the absorbance at wavelength 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between them was defined as the amount of blue formazan produced.

The calculation method of the epidermal keratinocyte proliferation promoting effect is as follows. The results are shown in Tables 30 to 32.

Epidermal keratinocyte growth rate (%) = St / Ct × 100

St: Absorbance in Cells Added Test Samples

Ct: absorbance in cells without addition of test sample

(Example 8: Recovery test from UV-B damage)

Using each of the plant fermentation extracts as a test sample, the recovery from UV-B damage was tested by the following test method.

Human normal dermal fibroblasts (NB1RGB) were cultured using α-MEM containing 10% FBS, and then cells were recovered by trypsinization. The recovered cells were diluted to a concentration of 2.0 × 10 ⁵ cells / mL using α-MEM, and then seeded in 200 μL per well in 48 well plates. After 24 hours of incubation, the medium was replaced with 100 μL of PBS (−) and irradiated with 1.0 J / cm 2 of UV-B. Immediately after irradiation, PBS (-) was removed immediately, and 400 µL of the test sample dissolved in 10% FBS-containing D-MEM was added to each well, followed by incubation for 24 hours. The recovery effect from ultraviolet UV-B damage was measured using an MTT assay. After completion of the culture, the medium was removed, and 200 μL of MTT dissolved in a final concentration of 0.4 mg / mL was added to each well. After incubation for 2 hours, the blue formazan produced in the cells was extracted with 200 μL of 2-propanol. After extraction, the absorbance at wavelength 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between them was defined as the amount of blue formazan produced. Similarly, the cells which did not irradiate UV-B after cell seeding and the cells which did not irradiate UV-B after cell seeding and did not add a test sample were measured in the same manner, and were set as non-irradiated groups and irradiated groups, respectively.

The calculation method of a UV-B damage recovery action is as follows. The results are shown in Tables 33 to 34.

UV-B damage recovery rate (%) = {(Nt-C)-(Nt-Sa)} / (Nt-C) × 100

Nt: absorbance in cells not irradiated with UV-B

C: absorbance in cells irradiated with UV-B and without addition of test sample

Sa: absorbance in cells irradiated with UV-B and with test sample

From the results of Examples 1 to 8, the extract of fermented mugwort, the extract of fermented bagus, the extract of fermented guava, the extract of fermented moth, the extract of fermented silverwood, the extract of fermented turmeric, and the extract of fermented moon Fibroblast proliferation, transglutaminase-1 production promotion, elastase activity inhibition, MMP-1 activity inhibition, estrogen-like action, type I collagen production promotion, epidermal keratinocyte growth promotion, and It has been confirmed that it has at least one of the action of recovery from UV-B damage, and from these facts, the extract of fermented mugwort, the extract of fermented bagasse, the extract of fermented guava, the extract of fermented moth, the extract of fermented silverwood, It has been suggested that the extract of fermented turmeric and the extract of fermented moonshine can be suitably used as an active ingredient of the anti-aging agent.

(Compound Example 1)

-latex-

The emulsion was prepared according to the following composition in a conventional manner.

· 50 mass% ethanol extract of fermented mugwort… 0.10 g

Jojoba oil… 4.00 g

1,3-butylene glycol... 3.00 g

Polyoxyethylene cetyl ether (20E.O.) 2.50 g

Olive oil… 2.00 g

· Squalene… 2.00 g

Cetanol 2.00 g

· Glyceryl monostearate… 2.00 g

Oleic acid polyoxyethylene sorbitan (20E.O.) 2.00 g

· Methyl paraoxybenzoate. 0.15 g

Astragalus extract… 0.10 g

· Potassium Diglyceride Glycerate… 0.10 g

Ginkgo leaf extract 0.10 g

Conchiolin... 0.10 g

Yellow-white extract… 0.10 g

Chamomile extract... 0.10 g

·Spices … 0.05 g

·Purified water … Remainder (total: 100.00 g)

(Combination Example 2)

-toilet water-

Toner was prepared according to the following composition in a conventional manner.

50 mass% ethanol extract of fermented bagasse. 0.10 g

· Glycerin… 3.00 g

1,3-butylene glycol... 3.00 g

Oleic acid polyoxyethylene sorbitan (20E.O.) 2.00 g

· Methyl paraoxybenzoate. 0.15 g

· Citric acid… 0.10 g

· Citric acid soda… 0.10 g

· Soluble Licorice Extract 0.10 g

Seaweed Extract… 0.10 g

· Ginseng extract… 0.10 g

Xylobiose mixture. 0.05 g

·Spices … 0.05 g

·Purified water … Remainder (total: 100.00 g)

(Combination Example 3)

-cream-

Creams were prepared in a conventional manner according to the following composition.

· 50 mass% ethanol extract of fermented guava. 0.10 g

· Squalene… 10.00 g

1,3-butylene glycol... 6.00 g

Flow paraffin 5.00 g

White Beeswax 4.00 g

Cetanol 3.00 g

· Glyceryl monostearate… 3.00 g

· Lanolin… 2.00 g

Oleic acid polyoxyethylene sorbitan (20E.O.) 1.50 g

· Methyl paraoxybenzoate. 1.50 g

· Stearic acid… 1.00 g

Yeast extract… 0.10 g

· Aperture extract… 0.10 g

· Basswood extract… 0.10 g

Oil extract (Oigi; Sanguisorba officinalis)... 0.10 g

·Spices … 0.10 g

·Purified water … Remainder (total: 100.00 g)

(Combination Example 4)

-pack-

The pack was prepared in a conventional manner according to the following composition.

· 50% by mass ethanol extract of fermented clover. 0.20 g

Polyvinyl alcohol 15.00 g

·ethanol … 10.00 g

Propylene glycol. 7.00 g

Polyethylene glycol... 3.00 g

Sage extract 0.10 g

· Angelica extract. 0.10 g

Ginseng extract 0.10 g

Ethyl paraoxybenzoate... 0.05 g

·Spices … 0.05 g

·Purified water … Remainder (total: 100.00 g)

(Compound Example 5)

Tablet Nutritional Supplements

The following mixture was compressed to prepare a dietary dietary supplement.

· 50 mass% ethanol extract of fermented herring tree. 30 g

· Bundang (sucrose). 178 g

Sorbite… 10 g

Glycerin fatty acid esters; 12 g

(Combination Example 6)

Granular Supplements

The following mixture was formed into a granular form to prepare a granular dietary supplement.

· 50 mass% ethanol extract of fermentation month. 20 g

Raffinose… 1000 g

Vitamin C... 167 g

Stevia extract 10 g

Since the anti-aging agent, the external preparation for skin and food and drink of the present invention have excellent anti-aging action, for example, the external skin preparation and food and beverage for the purpose of preventing and improving skin aging symptoms such as decreased skin elasticity, roughness, and wrinkles. It can be used suitably.

Claims (4)

It is characterized by containing one or more selected from extracts of fermented mugwort, extracts of fermented bagus, extracts of fermented guava, extracts of fermented moth, extracts of fermented silverwood, extracts of fermented turmeric, and extracts of fermented moonshine. Anti-aging agent. The method of claim 1, Promoting skin fibroblast growth, promoting transglutaminase-1 production, inhibiting elastase activity, inhibiting MMP-1 activity, estrogen-like action, promoting type I collagen production, promoting epidermal keratinocyte growth, And anti-aging agents having at least one of a recovery from UV-B damage. Anti-aging containing at least one selected from extracts of fermented mugwort, extracts of fermented bagasse, extracts of fermented guava, extracts of fermented moth, extract of fermented silverwood, extract of fermented turmeric, and extract of fermented moonshine A skin external preparation containing an agent. Anti-aging containing at least one selected from extracts of fermented mugwort, extracts of fermented bagasse, extracts of fermented guava, extracts of fermented moth, extract of fermented silverwood, extract of fermented turmeric, and extract of fermented moonshine A food and drink comprising an agent.