JP5797372B2 - Platelet aggregation inhibitor, stem cell growth factor (SCF) mRNA expression inhibitor, matrix metalloproteinase-1 (MMP-1) activity inhibitor, and matrix metalloproteinase-14 (MMP-14) activity inhibitor - Google Patents

Description

  The present invention relates to an antioxidant, an anti-inflammatory agent, a whitening agent, an anti-aging agent, a hair-restoring agent, an anti-obesity agent, and the antioxidant, anti-inflammatory agent, whitening agent, and anti-aging agent, which contain an extract of Nehwa The present invention relates to a cosmetic using at least one of an agent, a hair restorer, and an anti-obesity agent, and a food and drink.

In recent years, active oxygen has attracted attention as a factor that oxidizes biological components, and its adverse effect on living organisms has become a problem. Reactive oxygen is generated in the process of energy metabolism in living cells, and superoxide [that is, superoxide anion (· O ₂ −) generated by one-electron reduction of oxygen molecules, hydrogen peroxide (H ₂ O ₂ ), Singlet oxygen ( ¹ O ₂ ), hydroxy radical (.OH)], and the like. Such active oxygen is essential for the phagocytic sterilization mechanism and plays an important role in removing viruses and cancer cells.
However, excessive generation of the active oxygen attacks in vivo molecules constituting membranes and tissues in the living body and induces various diseases. Superoxide, which is produced in vivo and is a starting material for other active oxygen, is usually eliminated sequentially by the catalytic action of superoxide dismutase (SOD) contained in cells. However, when the production of superoxide is excessive or when the action of SOD is reduced, the superoxide is insufficiently erased and the superoxide concentration becomes high. This is one of the causes of tissue damage such as rheumatoid arthritis, Behcet's disease, myocardial infarction, stroke, cataract, spots, freckles, wrinkles, diabetes, arteriosclerosis, shoulder stiffness, coldness, skin aging, etc. It is considered.

Among these, since the skin is directly affected by environmental factors such as ultraviolet rays, superoxide is likely to be generated. Therefore, when the superoxide concentration is increased, biological tissues such as collagen are decomposed, denatured, or crosslinked. Or oxidize fats and oils to produce lipid peroxides that damage cells, forming skin wrinkles, causing skin aging, inflammation, and skin pigmentation (See Non-Patent Document 1).
Therefore, attempts have been made to obtain active oxygen scavenging substances, radical scavenging substances, hydrogen peroxide scavenging substances, etc. from natural products advantageous in terms of safety, and extracts of Brassica Brassica plants (see Patent Document 1), Efficacy has been confirmed for extracts of plants belonging to the genus Ryukyubekei (see Patent Document 2), extracts of scallops (see Patent Document 3), extracts of sulfur (see Patent Document 4), and the like.

  In addition, there are various causes and onset mechanisms of inflammatory diseases such as contact dermatitis (rash), psoriasis, pemphigus vulgaris, and various other skin diseases with rough skin. As the cause thereof, for example, nitric oxide (NO) production and platelet aggregation are known.

  The platelet aggregation leads to activation of phospholipase A2 in the arachidonic acid cascade, whereby leukotriene B, prostaglandin E2, and the like are released and become a inflammation substance. For this reason, attempts have been made to prevent or treat allergic diseases and inflammatory diseases with substances that inhibit or suppress platelet aggregation. Examples of extracts of herbal medicines having an inhibitory action on platelet aggregation include, for example, an extract of a plant belonging to the genus Canalium (see Patent Document 5), an extract of Kusasanfu (see Patent Document 6), and a Fuji tea extract (Patent Document). 7)) and the like have been reported.

Melanin also plays a role in protecting the body from ultraviolet rays in the skin, but overproduction and uneven accumulation cause skin darkening and spots. In general, melanin is changed from tyrosine to dopa and from dopa to dopaquinone by the action of an enzyme tyrosinase biosynthesized in pigment cells, and then formed through an intermediate such as 5,6-dihydroxyindophenol. Therefore, in order to prevent and improve skin darkness (skin pigmentation), that is, for whitening, it is effective to inhibit the melanin production process or to lightly bleach already produced melanin. It is done.
As herbal medicines having such a tyrosinase inhibitory action, for example, Fuji tea extract (see Patent Document 8), Yanagita extract (see Patent Document 9) and the like have been reported.

In addition, the development of whitening agents so far has been focused on tyrosinase, the rate-limiting enzyme for melanin production. Recently, production from epidermal keratinocytes increased after irradiation with ultraviolet UV-B, and pigment cells (melanocytes) Α-melanocyte-stimulating hormone (α-MSH), endothelin-1 (ET-1), nitric oxide, basic fibroblast growth factor (bFGF), granulocyte / macrophage / colony stimulating factor (GM-CSF), stem cell growth factor (SCF), etc. have been reported. By blocking the information transmission system in which these are involved, development of a substance that suppresses melanin production and leads to a whitening effect is actively performed. It has become like this.
The stem cell growth factor (Stem Cell Factor, SCF) is a protein produced from keratinocytes, fibroblasts, vascular endothelial cells, bone marrow stromal cells and the like. SCF is known to have an action of promoting proliferation and differentiation of pluripotent hematopoietic stem cells, germ cells, mast cells, megakaryocyte precursor cells, granulocyte / macrophage precursor cells, pigment cells and the like. Moreover, it is known that the expression of SCF is enhanced by a spot site, ultraviolet irradiation or the like (see Non-Patent Document 2). As SCF, a membrane-bound SCF composed of 273 amino acid residues and a secreted SCF that is cleaved by the action of a proteolytic enzyme and released from the membrane are known. Membrane-bound SCF binds to the SCF receptor of the pigment cell while bound to keratinocytes and promotes the proliferation of the pigment cell. Secreted SCF is cleaved at the binding site, released from the cell membrane, and bound to the SCF receptor of the pigment cell, thereby promoting proliferation of the pigment cell.
Therefore, abnormal production of SCF is thought to lead to abnormal growth of pigment cells, increase melanin production, and cause stains, buckwheat, dullness, and the like.
Therefore, suppressing the increase in the expression of SCF suppresses the proliferation of pigment cells, suppresses the excessive production of melanin in the skin, and is considered useful for the prevention or suppression of pigmentation, sun spots, buckwheat, etc. after sunburn. It is done.

The epidermis and dermis of the skin are composed of epidermal cells, dermal fibroblasts, and an extracellular matrix such as collagen that is outside these cells and supports the skin structure. In young skin, the interaction between these skin tissues is kept constant, so that moisture retention, flexibility, elasticity, etc. are secured, and the skin is maintained in a fresh state with its tension and gloss. .
However, cells are affected by external factors such as ultraviolet (UV-A, UV-B) irradiation, significant drying of air, excessive skin washing, contact with hydrogen peroxide, etc., or aging progresses. The production amount of collagen, which is a main component of the outer matrix, is reduced, and the elasticity is reduced by crosslinking. As a result, the skin's moisturizing function and elasticity are lowered, and the horny layer begins to exfoliate abnormally, so that the skin loses its tension and gloss, and exhibits aging symptoms such as roughness and wrinkles. Furthermore, when the growth rate of fibroblasts decreases with the influence of external factors and aging, the synthesis amount of hyaluronic acid, which is a natural moisturizing factor, decreases.
As described above, it is known that changes accompanying aging of the skin, that is, wrinkles, dullness, disappearance of texture, decrease in elasticity, and the like are associated with reduction or modification of dermal matrix components such as collagen.
Among collagens, type I collagen is the most abundant collagen contained in the body and is also abundant in the dermis of the skin and is known to play a role in producing the strength of the skin.

In recent years, as a factor for inducing reduction or denaturation of dermal matrix components, there is degradation and reconstruction of a group of proteolytic enzymes called matrix metalloproteases (hereinafter also referred to as “MMPs”).
The MMPs are classified into (1) collagenase group (MMP-1, MMP-8 and MMP-13), (2) gelatinase group (MMP-2 and MMP-9), (1) due to differences in primary structure and substrate specificity. 3) Stromelysin group (MMP-3 and MMP-10), (4) Membrane-bound matrix metalloprotease group (MMP-14, MMP-15, MMP-16, and MMP-17), (5) Others ( MMP-7, MMP-11, and MMP-12) (see Patent Document 10).
Among the MMPs, MMP-1 and MMP-14 are known as enzymes that degrade type I collagen, type II collagen, and type III collagen, which are main components of the dermal matrix of the skin. In addition, the expression is greatly increased by the irradiation of ultraviolet rays, which contributes to decrease or denaturation of collagen by ultraviolet rays, and is considered to be a major factor such as the formation of wrinkles on the skin.

  In addition, as a cause of skin aging associated with aging, secretion of estrogen, a type of female hormone, may decrease. Estrogen is deeply involved in maintaining the health of adult women, and its lack of secretion leads to various medical illnesses and can cause unwanted skin changes such as skin sensitivities, reduced elasticity and reduced moisture. It has been known.

Therefore, the formation of wrinkles in the skin, the decrease in elasticity, etc. are compensated by inhibiting matrix metalloproteinase-1 (MMP-1) activity, inhibiting matrix metalloproteinase-14 (MMP-14) activity, or decreasing estrogen secretion due to aging. It is thought that skin aging can be prevented or treated.
So far, as estrogenic agents, for example, steroidal estrogens, nonsteroidal estrogens, flavone compounds (see Patent Documents 11 to 13) and the like have been reported.

In addition, many steroid hormones are expressed in the molecular form secreted from the production organ and bind to the receptor to express its action. In the case of male hormones collectively called androgens, for example, testosterone enters the cells of the target organ. Then, it is reduced to dihydrotestosterone by testosterone 5α-reductase, and then binds to a receptor to express an action as an androgen.
The androgen is an important hormone, but if it acts excessively, it can be affected by various factors such as androgenetic alopecia, hirsutism, seborrhea, acne (such as acne), benign prostatic hyperplasia, prostate tumor, premature male sexuality, etc. Induces undesirable symptoms.

Therefore, conventionally, in order to improve these various symptoms, a method for suppressing the action of excess androgen, specifically, by inhibiting the action of testosterone 5α-reductase that reduces testosterone to dihydrotestosterone, There have been proposed a method for suppressing the occurrence and a method for preventing the expression of androgen activity by inhibiting the binding of dihydrotestosterone generated from testosterone to the receptor.
For example, an extract of the genus Chorospondias genus plant (see Patent Document 14) has been reported as having such an action of inhibiting the action of testosterone 5α-reductase (testosterone 5α-reductase activity inhibiting action). .
In addition, examples of substances having an action of inhibiting the binding of dihydrotestosterone to a receptor (androgen receptor binding inhibitory action) include, for example, at least one extract of majito and cuta (see Patent Document 15). Has been.

In order to prevent obesity, it is considered effective to suppress the action of cyclic AMP phosphodiesterase, which is an enzyme that degrades cyclic AMP involved in promoting fat metabolism. In fact, it is known that when the action of cyclic AMP phosphodiesterase is suppressed, the concentration of intracellular cyclic AMP increases, lipid metabolism becomes active, and obesity is resolved.
As a substance having such a cyclic AMP phosphodiesterase activity inhibitory effect, for example, Fuji tea extract (see Patent Document 16) has been reported.

  However, until now, however, it is a natural product that is easy to obtain, inexpensive, and highly safe, and does not adversely affect the quality of the additive in terms of taste, smell, feeling of use, etc. Antioxidants, anti-inflammatory agents, whitening agents, anti-aging agents, hair-restoring agents, and anti-obesity agents that can be widely used in foods and foods and drinks have not been provided yet, and their prompt provision is strongly demanded. Is the current situation.

JP 2003-81848 A JP 2005-29483 A JP 2006-321730 A JP 2007-8902 A JP 2002-53478 A JP 2002-53477 A JP 2001-97873 A JP 2002-370962 A JP 2004-083488 A JP 2000-344672 A JP 2002-226323 A JP 2001-316240 A JP 2003-55245 A JP 2003-55162 A JP 2002-241297 A JP 2003-12532 A

“Fragrance Journal” Special Issue No. 14, p156, 1995 Hachiya A et al. , J. et al. Invest. Dermatol. , No. 116, 2001, p. 578-586

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention has an excellent antioxidant action and a highly safe antioxidant, an excellent anti-inflammatory action, and a highly safe anti-inflammatory agent, an excellent whitening action, A highly safe whitening agent, an excellent anti-aging effect, a highly safe anti-aging agent, an excellent hair restoring action, a highly safe hair restoring agent, and an excellent anti-obesity effect And anti-obesity agents with high safety, and cosmetics and foods and beverages using at least one of the antioxidants, anti-inflammatory agents, whitening agents, anti-aging agents, hair-restoring agents, and anti-obesity agents The purpose is to provide.

The present inventors have conducted extensive studies to solve the above problems, an extract of Hehuan (Albizzia julibrissin) has excellent antioxidant, anti-inflammatory, whitening effect, anti-aging effect, hair growth effect, And it has been found that it has an anti-obesity action, and has completed the present invention.

The present invention is based on the above findings of the present inventors, and means for solving the above problems are as follows. That is,
<1> An antioxidant containing an extract of Albizia julibrissin .
<2> The antioxidant according to <1>, which has at least one of a superoxide scavenging action and a radical scavenging action.
<3> An anti-inflammatory agent characterized by containing an extract of Albizia julibrissin .
<4> The anti-inflammatory agent according to <3>, which has a platelet aggregation inhibitory action.
<5> A whitening agent characterized by containing an extract of Albizizza julibrissin .
<6> The whitening agent according to <5>, which has at least one of a tyrosinase activity inhibitory action and a stem cell growth factor (SCF) mRNA expression inhibitory action.
<7> An anti-aging agent characterized by containing an extract of Albizia julibrissin .
<8> At least one of matrix metalloproteinase-1 (MMP-1) activity inhibitory action, matrix metalloproteinase-14 (MMP-14) activity inhibitory action, estrogen-like action, superoxide scavenging action, and radical scavenging action The anti-aging agent according to <7>.
<9> A hair restorer comprising an extract of Albizia julibrissin .
<10> The hair restorer according to <9>, which has at least one of a testosterone 5α-reductase activity inhibitory action and an androgen receptor binding inhibitory action.
<11> An anti-obesity agent comprising an extract of Albizia julibrissin .
<12> The antiobesity agent according to <11>, which has a cyclic AMP phosphodiesterase activity inhibitory action.
<13> The antioxidant according to any one of <1> to <2>, the anti-inflammatory agent according to any one of <3> to <4>, and any one of <5> to <6> Whitening agent according to <7> to <8> above, anti-aging agent according to any of <9> to <10> above, and <11> to <12> above A cosmetic comprising at least one of the anti-obesity agents according to any one of the above.
<14> The antioxidant according to any one of <1> to <2>, the anti-inflammatory agent according to any one of <3> to <4>, and any one of <5> to <6> Whitening agent according to <7> to <8> above, anti-aging agent according to any of <9> to <10> above, and <11> to <12> above A food or drink comprising at least one of the anti-obesity agents according to any one of the above.

  According to the present invention, it is possible to solve the conventional problems, achieve the above-mentioned object, have an excellent antioxidant action, and have a high safety, an excellent anti-inflammatory action, and Highly safe anti-inflammatory agent, excellent whitening effect, high safety whitening agent, excellent anti-aging effect, high safety anti-aging agent, excellent hair growth action, And a highly safe hair restoring agent, an anti-obesity agent having excellent anti-obesity action and high safety, and the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restoring agent, and anti-anti-obesity agent Cosmetics using at least one of the obesity agents and foods and drinks can be provided.

(Antioxidants, anti-inflammatory agents, whitening agents, anti-aging agents, hair restorers, and anti-obesity agents)
The antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent of the present invention comprise an extract of Albizia julibrissin , and further contain other ingredients as necessary. It contains.

The antioxidant has an antioxidant action based on at least one of a superoxide scavenging action and a radical scavenging action.
The anti-inflammatory agent has an anti-inflammatory action based on a platelet aggregation inhibitory action.
The whitening agent has a whitening action based on at least one of a tyrosinase activity inhibitory action and a stem cell growth factor (SCF) mRNA expression inhibitory action.
The anti-aging agent is at least one of matrix metalloproteinase-1 (MMP-1) activity inhibitory action, matrix metalloproteinase-14 (MMP-14) activity inhibitory action, estrogen-like action, superoxide scavenging action, and radical scavenging action It has anti-aging action based on crab.
The hair restoring agent has a hair restoring action based on at least one of a testosterone 5α-reductase activity inhibitory action and an androgen receptor binding inhibitory action.
The anti-obesity agent has an anti-obesity action based on a cyclic AMP phosphodiesterase activity inhibitory action.
Wherein contained in the extract of Hehuan (Albizzia julibrissin), antioxidant, anti-inflammatory, whitening effect, anti-aging effects, it is unclear details of hair restoring action, and exhibits at least one anti-obesity agent However, the extract of the above-mentioned Albizzia julibrissin has such an excellent action and is useful as an antioxidant, an anti-inflammatory agent, a whitening agent, an anti-aging agent, a hair-restoring agent, and an anti-obesity agent. This is a new finding by the present inventors that has not been known at all.

Said Nemu (Albizzia julibrissin) refers to the alias both Albizzia Julibrissin, is a leguminous plant, China Zhejiang, cheap fine, Jiangsu, are distributed in Sichuan, etc., it is readily available from these areas.
There is no particular limitation on the site of the cheering used as the extraction raw material, and it can be appropriately selected according to the purpose. For example, flowers, persimmons, fruits, pericarps, seeds, seed coats, stems, leaves, branches, branches and leaves, Examples thereof include trunk, bark, root, rhizome, root bark, and a mixture thereof. Among these, flowers are preferable.

  For example, the above-mentioned soup that is an extraction raw material can be subjected to solvent extraction in a state of being dried or pulverized using a crusher or the like. Among them, the extraction raw material is preferably dried and pulverized immediately after collection. The drying may be performed, for example, in the sun or using a commonly used dryer. In addition, you may use the said welcome as an extraction raw material, after giving pretreatments, such as a degreasing | defatting, by nonpolar solvents, such as hexane and benzene. By performing pretreatment such as degreasing, the extraction treatment with the above-mentioned polar solvent can be performed efficiently.

  The extract of the joy can be easily obtained by using a method generally used for plant extraction. Moreover, you may use a commercial item as an extract of the said comfort. It should be noted that the above-mentioned extract of the cheers includes the extract of the cheers, the diluted or concentrated solution of the extract, the dried product of the extract, or any of these roughly purified products or purified products.

  There is no restriction | limiting in particular as a solvent used for the said extraction, According to the objective, it can select suitably, For example, water, a hydrophilic organic solvent, or these mixed solvents are room temperature or the temperature below the boiling point of a solvent. It is preferable to use it. Ingredients that exhibit at least one of the antioxidative action, anti-inflammatory action, whitening action, anti-aging action, hair growth action, and anti-obesity action contained in the above-mentioned cheer are easily extracted by an extraction process using a polar solvent as an extraction solvent. can do.

  The water that can be used as the extraction solvent is not particularly limited and can be appropriately selected depending on the purpose. For example, pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, etc. In addition, those subjected to various treatments are included. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  The hydrophilic organic solvent that can be used as the extraction solvent is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include lower ones having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol. Alcohols: lower aliphatic ketones such as acetone and methyl ethyl ketone; polyhydric alcohols having 2 to 5 carbon atoms such as 1,3-butylene glycol, propylene glycol, glycerin, etc., and a mixed solvent of the hydrophilic organic solvent and water Etc. can also be used. In addition, when using the mixed solvent of the said water and the said hydrophilic organic solvent, in the case of a lower alcohol, 1 mass part-90 mass parts with respect to 10 mass parts of water, and the water 10 in the case of a lower aliphatic ketone. It is preferable to use what mixed 1 mass part-40 mass parts with respect to the mass part. Moreover, in the case of polyhydric alcohol, it is preferable to use what mixed 1 mass part-90 mass parts with respect to 10 mass parts of water.

A special extraction method is employed to extract an extract having at least one of an antioxidant action, an anti-inflammatory action, a whitening action, an anti-aging action, a hair-growth action, and an anti-obesity action from the above-mentioned soup that is an extraction raw material. It is not necessary, and extraction can be performed using any extraction device at room temperature or under reflux heating.
Specifically, each of the extraction raw materials is put into a treatment tank filled with an extraction solvent, and the mixture is allowed to stand for 30 minutes to 4 hours with occasional stirring as necessary to elute soluble components, followed by filtration. Then, the solid matter is removed, the extraction solvent is distilled off from the obtained extract, and the extract can be obtained by drying. The amount of extraction solvent is usually 5 to 15 times (mass ratio) of the extraction raw material. The extraction conditions are usually about 1 to 4 hours at 50 to 95 ° C. when water is used as the extraction solvent. Moreover, when the mixed solvent of water and ethanol is used as an extraction solvent, it is normally 30 minutes-about 4 hours at 40 to 80 degreeC. In addition, the extract obtained by extracting with a solvent, as long as the extraction solvent is highly safe, as it is, the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-oxidant of the present invention. It can be used as an active ingredient of an obesity agent.

  In order to obtain a dilute or concentrated solution of the extract, a dried product of the extract, or a crude purified product or a purified product thereof, the extract of the funeral obtained by extraction is diluted, concentrated, dried according to a conventional method. Treatment such as purification may be performed. In addition, the obtained soy sauce extract can be used as an active ingredient of an antioxidant, an anti-inflammatory agent, a whitening agent, an anti-aging agent, a hair-restoring agent, and an anti-obesity agent as it is. The dried product is easier to use. In obtaining the dried extract, a conventional method can be used, and carriers such as dextrin and cyclodextrin may be added to improve hygroscopicity. In addition, the soy sauce that is an extraction raw material may have a peculiar smell and taste, and therefore, the soy sauce extract may be decolorized, deodorized, etc. within a range that does not cause a decrease in physiological activity. Although it is possible to carry out the desired purification, for example, when it is added to cosmetics, it is not used in a large amount. Specifically, purification can be performed by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like.

The extract of the above-mentioned funeral obtained as described above has a superoxide scavenging action, radical scavenging action, platelet aggregation inhibitory action, tyrosinase activity inhibitory action, stem cell growth factor (SCF) mRNA expression inhibitory action, matrix metalloproteinase-1 ( MMP-1) activity inhibitory action, matrix metalloproteinase-14 (MMP-14) activity inhibitory action, estrogen-like action, testosterone 5α-reductase activity inhibitory action, androgen receptor binding inhibitory action, and cyclic AMP phosphodiesterase activity inhibitory action Based on these actions, it can be suitably used as an active ingredient of at least one of the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent of the present invention. Is something.
It should be noted that the above-mentioned extract of joy is based on each of the above-mentioned actions, and is based on the above-described actions, superoxide scavenger, radical scavenger, platelet aggregation inhibitor, tyrosinase activity inhibitor, stem cell growth factor (SCF) mRNA expression inhibitor, matrix metalloproteinase- 1 (MMP-1) activity inhibitor, matrix metalloproteinase-14 (MMP-14) activity inhibitor, estrogen-like agent, testosterone 5α-reductase activity inhibitor, androgen receptor binding inhibitor, and cyclic AMP phosphodiesterase activity Inhibitors can also be preferably used.

The antioxidant action in the antioxidant of the present invention is exhibited based on at least one of a superoxide scavenging action and a radical scavenging action.
The anti-inflammatory action of the anti-inflammatory agent of the present invention is exhibited based on the platelet aggregation inhibitory action.
The whitening action of the whitening agent of the present invention is exhibited based on at least one of a tyrosinase activity inhibitory action and a stem cell growth factor (SCF) mRNA expression inhibitory action.
The anti-aging action of the anti-aging agent of the present invention includes matrix metalloproteinase-1 (MMP-1) activity inhibitory action, matrix metalloproteinase-14 (MMP-14) activity inhibitory action, estrogen-like action, superoxide scavenging action, and It is exhibited based on at least one of radical scavenging action.
According to the anti-aging action based on the superoxide scavenging action and the radical scavenging action, for example, it is possible to suppress the formation of wrinkles on the skin and the decrease in the elasticity of the skin due to the active oxygen generated excessively in the skin.
The hair-restoring effect of the hair-restoring agent of the present invention is exhibited based on at least one of a testosterone 5α-reductase activity inhibiting action and an androgen receptor binding inhibiting action.
The anti-obesity action of the anti-obesity agent of the present invention is exhibited based on the cyclic AMP phosphodiesterase activity inhibitory action.

  The content of the joy extract in the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, anti-obesity agent is not particularly limited and can be appropriately selected depending on the purpose. In addition, the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent may be the so-called “excitement extract” itself.

In addition, the effects of the present invention are not impaired as other components other than the above-mentioned extract of joy that can be contained in the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent. If it is in the range, there is no particular limitation, and it can be appropriately selected according to the purpose. For example, a physiological saline solution for diluting the extract of the joy to a desired concentration can be mentioned. Further, the content of the other components in the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent is not particularly limited and may be appropriately selected depending on the purpose. it can.
In addition, the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair-restoring agent, anti-obesity agent can be formulated into powders, granules, tablets, etc. in any dosage form by formulating as necessary. It can be.

  The antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer and anti-obesity agent of the present invention have excellent antioxidant action, anti-inflammatory action, whitening action, anti-aging action, hair growth action and anti-obesity action. In addition to being excellent in safety, it is suitable for use in, for example, the cosmetics of the present invention described later and the food and drink of the present invention.

(Cosmetics)
The cosmetic of the present invention contains at least one of the above-described antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent of the present invention. It contains.
The cosmetic may be a composition in which the extract of the cheer is blended with an arbitrary cosmetic so as not to hinder the activity thereof, or may be a cosmetic mainly composed of the extract of the cheer. Good. In addition, the cosmetic may be the comforting extract itself.

  The cosmetic of the present invention has high safety and has excellent superoxide scavenging action, radical scavenging action, platelet aggregation inhibitory action, tyrosinase activity inhibitory action, stem cell growth factor (SCF) mRNA expression inhibitory action, matrix metalloprotease- 1 (MMP-1) activity inhibitory action, matrix metalloproteinase-14 (MMP-14) activity inhibitory action, estrogen-like action, testosterone 5α-reductase activity inhibitory action, androgen receptor binding inhibitory action, and cyclic AMP phosphodiesterase activity inhibitory action It exhibits at least one of the actions.

Here, there is no restriction | limiting in particular as said cosmetics, According to the objective, it can select suitably, For example, skin cosmetics, hair cosmetics, etc. are mentioned.
The use of the cosmetic is not particularly limited and can be appropriately selected from various uses. For example, ointment, cream, emulsion, lotion, lotion, pack, jelly, lip balm, lipstick, bath preparation, astringent, Hair tonic, shampoo, rinse etc. are mentioned.

If necessary, the cosmetic can further contain various main ingredients and auxiliaries usually used in the production of the cosmetic and other components as long as the objects and effects of the present invention are not impaired.
The other components are not particularly limited and may be appropriately selected depending on the intended purpose. For example, astringents, bactericides, antibacterial agents, whitening agents, ultraviolet absorbers, humectants, cell activators, fats and oils Waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, and the like.

  The content of at least one of the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent in the cosmetic is not particularly limited, and the type and extraction of the cosmetic Although it can adjust suitably according to the physiological activity etc. of a thing, 0.0001 mass%-10 mass% are preferable as an amount of the extract of the said comfort, for example, 0.001 mass%-5 mass% are more preferable.

(Food)
The food / beverage product of the present invention contains at least one of the above-described antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent of the present invention. It contains.
Here, the food and drink are those which are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to the category of goods, etc., and means, for example, a wide range of foods that are taken orally, such as general foods, health foods, health functional foods, quasi drugs, and pharmaceuticals.
The food or drink may be any food or drink blended with any extract of the cheer extract so as not to interfere with its activity. Also good. In addition, the food and drink may be the joy extract itself.

  The food / beverage product of the present invention has high safety and excellent superoxide scavenging action, radical scavenging action, platelet aggregation inhibitory action, tyrosinase activity inhibitory action, stem cell growth factor (SCF) mRNA expression inhibitory action, matrix metalloprotease- 1 (MMP-1) activity inhibitory action, matrix metalloproteinase-14 (MMP-14) activity inhibitory action, estrogen-like action, testosterone 5α-reductase activity inhibitory action, androgen receptor binding inhibitory action, and cyclic AMP phosphodiesterase activity inhibitory action It exhibits at least one of the actions.

  There is no restriction | limiting in particular as said food-drinks, According to the objective, it can select suitably, For example, drinks, such as a soft drink, a carbonated drink, a nutrition drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet, shaved ice, etc. Noodles such as buckwheat noodles, udon, harusame, gyoza skin, sweet husk, Chinese noodles, instant noodles; rice cakes, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, Sweets such as bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red sea bream, scallops, abalone, sea urchin, crabs, tocobushi, etc .; kamaboko, ham, sausage Processed fishery and livestock products such as dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, and short nibs Fats and processed oils such as whipped cream and dressings; seasonings such as sauces and sauces; curry, stew, oyakodon, rice cakes, miso cooked rice, Chinese rice cakes, and rice cakes, tempura, eel rice, hayashi rice, oden, marvodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger, meatballs; various forms of health foods and nutritional supplements; pharmaceuticals such as tablets, capsules, drinks, troches, etc. Examples include foreign products.

There is no restriction | limiting in particular as said other component, According to the objective, it can select suitably, For example, the auxiliary | assistant raw material or additive etc. which are normally used in manufacturing food-drinks are mentioned.
The auxiliary raw material or additive is not particularly limited and may be appropriately selected depending on the intended purpose. For example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid , Tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, Arabia Examples include gum, carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives and the like.

  The content of at least one of the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent in the food / beverage product varies depending on the type of the food / beverage product. For example, when it is intended to be blended in any food or drink within a range that does not impair the original taste of the food or drink, the amount of the extract of the cheerful ingredient that is an active ingredient cannot be specified. As 0.001 mass%-50 mass% are preferable, and 0.01 mass%-20 mass% are more preferable. In addition, for example, when the purpose is to produce nutritional supplement foods and drinks such as granules, tablets, capsules and the like mainly composed of the extract of Nehwa, the amount of the Nehwa extract as an active ingredient 0.01 mass% to 100 mass% is preferable, and 5 mass% to 100 mass% is more preferable.

(effect)
The antioxidants, anti-inflammatory agents, whitening agents, anti-aging agents, hair-restoring agents, anti-obesity agents, cosmetics and foods and beverages of the present invention can be used on a daily basis and are active ingredients. By the action of the extract of joy, at least one of an antioxidant action, an anti-inflammatory action, a whitening action, an anti-aging action, a hair growth action and an anti-obesity action can be exhibited extremely effectively.

  The antioxidants, anti-inflammatory agents, whitening agents, anti-aging agents, hair-restoring agents, anti-obesity agents, and cosmetics and foods and beverages of the present invention are preferably applied to humans. It is also possible to apply to animals other than humans (for example, mice, rats, hamsters, dogs, cats, cows, pigs, monkeys, etc.) as long as their effects are exhibited. Further, the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair-restoring agent, anti-obesity agent, and cosmetics and foods and beverages of the present invention have the above-mentioned extract of natural fun as an active ingredient. It is also advantageous in that it is excellent in safety.

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

(Production Example 1)
-Manufacture of water extract of Albizia julibrissin-
Ten times the amount of water by mass ratio was added to the crushed flower of the cheering flowers, heated at 80 ° C. for 2 hours, and filtered. The same amount of water was added to the residue, heated at 80 ° C. for 2 hours, and filtered. The filtrate was concentrated and freeze-dried to obtain a water extract (freeze-dried product). In addition, the extraction rate of the obtained water extract of Nehwa was 31.5%.

(Production Example 2)
- preparation of 50 wt% ethanol extract of Hehuan (Albizzia julibrissin) -
Ten masses of 50% by mass of ethanol was added to the crushed flower of Nehwa, heated at 80 ° C. for 2 hours, and filtered. The same amount of 50% by mass ethanol was added to the residue, heated at 80 ° C. for 2 hours, and filtered. The filtrate was concentrated and freeze-dried to obtain a 50% by weight ethanol extract (freeze-dried product). In addition, the extraction rate of the obtained 50% by mass ethanol extract of Nehwa was 29.8%.

(Production Example 3)
-Production of 80% by weight ethanol extract of Albizia julibrissin-
10 masses (mass ratio) of 80% by mass ethanol was added to the pulverized flower of Nehwa, heated at 80 ° C. for 2 hours, and filtered. The same amount of 80% by mass ethanol was added to the residue, heated at 80 ° C. for 2 hours, and filtered. The filtrate was concentrated and freeze-dried to obtain a 80% by weight ethanol extract (freeze-dried product). In addition, the extraction rate of the obtained 80% by mass ethanol extract of Nehwa was 26.9%.

( Reference Example 1: Superoxide scavenging action test (NBT method))
The superoxide scavenging action was tested by the following test method using the extract of each cheers obtained in Production Examples 1 to 3 as a test sample.

3 mmol / L xanthine, 3 mmol / L EDTA, 1.5 mg / mL bovine serum albumin (BSA) solution, 0.75 mmol / L nitroblue tetrazolium (NBT) 0.1 mL each, and 0.05 mol / L 2.4 mL of Na ₂ CO ₃ buffer (pH 10.2) was placed in a test tube, 0.1 mL of each sample solution was added thereto, and the mixture was allowed to stand at 25 ° C. for 10 minutes. Subsequently, the xanthine oxidase solution was added, it stirred rapidly, and it left still at 25 degreeC for 20 minutes. Thereafter, 0.1 mL of 6 mmol / L copper chloride was added to stop the reaction, and the absorbance at a wavelength of 560 nm was measured. The absorbance measured at this time was defined as “absorbance upon addition of sample solution and enzyme solution”.
The same operation and measurement of absorbance were performed without adding the enzyme solution. The absorbance measured at this time was defined as “absorbance when the sample solution was added and no enzyme solution was added”.
The same measurement was performed when distilled water was added without adding the sample solution. The absorbance measured at this time was defined as “absorbance when no sample solution was added and enzyme solution was added”.
Moreover, the same measurement was performed when distilled water was added without adding the enzyme solution and without adding the sample solution. The absorbance measured at this time was defined as “absorbance when no sample solution was added and no enzyme solution was added”.
And the superoxide elimination rate was calculated | required by following Numerical formula 1 from the measurement result. The results are shown in Table 1. The test samples were used at sample concentrations of 100 μg / mL, 50 μg / mL, and 25 μg / mL.
<Formula 1>
Superoxide erasure rate (%) = {1- (AB) / (CD)} × 100
A: Absorbance when sample solution is added and enzyme solution is added B: Absorbance when sample solution is added and enzyme solution is not added C: Absorbance when sample solution is not added and enzyme solution is added D: When sample solution is not added and enzyme solution is not added Absorbance of

Next, the superoxide erasure rate is measured by gradually reducing the sample concentration, and the sample concentration at which the superoxide erasure rate becomes 50% (hereinafter sometimes referred to as “IC ₅₀ ”) (μg). / ML) was determined by interpolation. The results are shown in Table 2.

表１から２の結果から、製造例１から３の合歓の抽出物が、中程度のスーパーオキサイド消去作用を有することが確認できた。

From the results of Tables 1 and 2, it was confirmed that the extract of Nehwa in Production Examples 1 to 3 had a moderate superoxide scavenging action.

( Reference Example 2: Radical scavenging action test for DPPH)
Using each of the extracts of the cheers obtained in Preparation Examples 1 to 3 as test samples, 1,1-diphenyl-2-picrylhydradyl radical (DPPH), which is a very stable radical, was used according to the following test method. The radical scavenging action was tested.

3 mL of each sample solution was added to 3 mL of 1.5 × 10 ⁻⁴ mol / L DPPH ethanol solution, and the container was immediately sealed and shaken and allowed to stand for 30 minutes, and then the absorbance at a wavelength of 520 nm was measured.
As a control, the same operation was performed using a solvent in which the sample solution was dissolved instead of the sample solution, and the absorbance at a wavelength of 520 nm was measured. Further, as a blank, after adding 3 mL of the sample solution to ethanol, the absorbance at a wavelength of 520 nm was measured immediately.
And from the measurement result, radical elimination rate (%) was calculated | required by following Numerical formula 2. The results are shown in Table 3. The test samples were used at sample concentrations of 200 μg / mL, 100 μg / mL, and 50 μg / mL.
<Formula 2>
Radical scavenging rate (%) = {1− (BC) / A} × 100
A: Absorbance of control B: Absorbance when sample solution is added C: Absorbance of blank

Next, the radical scavenging rate is measured by decreasing the sample concentration step by step, and the sample concentration at which the DPPH radical scavenging rate is 50% (hereinafter sometimes referred to as “IC ₅₀ ”) (μg / mL) was determined by interpolation. The results are shown in Table 4.

表３から４の結果から、製造例１から３の合歓の抽出物が、ＤＰＰＨに対するラジカル消去作用を有することが確認できた。

From the results of Tables 3 to 4, it was confirmed that the extract of Nehwa in Production Examples 1 to 3 had a radical scavenging action on DPPH.

The results from Example 1 2, extract of Hehuan (Albizzia julibrissin) is superoxide scavenging action, and was confirmed to have at least one radical scavenging action, an extract of Hehuan (Albizzia julibrissin), anti It was suggested that it can be suitably used as an active ingredient of an oxidizing agent and an anti-aging agent.

(Example 3: platelet aggregation inhibitory action test)
Using the extract of each cheer obtained in Production Examples 1 to 3 as a test sample, the platelet aggregation inhibitory action was tested by the following test method.

Rabbit blood collected by adding 1/10 volume of Japanese Pharmacopoeia heparin sodium injection solution was centrifuged (180 × g, 10 minutes, room temperature) to obtain a platelet suspension (PRP). This was used as a platelet suspension. 1 μL of 200 mmol / L calcium chloride solution was added to 223 μL of platelet suspension and reacted at 37 ° C. for 1 minute. Add 1 μL of the test sample solution to this, react for another 2 minutes, add a stir bar and stir for 1 minute, add 25 μL of collagen solution, and perform aggregation for 10 minutes at 37 ° C. Platelet aggregation measuring device PAM12CL (Mebanics Corporation) Was used to measure the agglomeration rate (A). Separately, the solvent of the sample solution was added instead of the sample solution, the same operation as described above was performed, the aggregation rate (B) was measured, and the platelet aggregation inhibition rate was determined by the following formula 3. The results are shown in Table 5. The test samples were tested at sample concentrations of 400 μg / mL and 100 μg / mL.
<Formula 3>
Platelet aggregation inhibition rate (%) = {(A−B) / A} × 100
A: Platelet aggregation rate of control B: Platelet aggregation rate upon addition of test sample solution

表５の結果から、製造例１から３の合歓の抽出物が、血小板凝集抑制作用を有することが確認できた。

From the results of Table 5, it was confirmed that the extract of Nehwa of Production Examples 1 to 3 had a platelet aggregation inhibitory action.

From the results of Example 3, extract of Hehuan (Albizzia julibrissin), it was confirmed to have a platelet aggregation inhibitory action, an extract of Hehuan (Albizzia julibrissin) is, as an active ingredient of anti-inflammatory agents, suitably used It was suggested that

( Reference Example 4: Tyrosinase activity inhibitory action test)
Using the extract of each cheer obtained in Production Examples 1 to 3 as a test sample, the tyrosinase activity inhibitory action was tested by the following test method.

To a 48-well plate, 0.2 mL of Mclvaine buffer (pH 6.8), 0.06 mL of 0.3 mg / mL tyrosine solution, and 0.18 mL of 25% DMSO solution of the test sample were added, and allowed to stand at 37 ° C. for 10 minutes. To this, 2,500 units / mL tyrosinase solution 0.02 mL was added, followed by reaction at 37 ° C. for 15 minutes. After completion of the reaction, the absorbance at a wavelength of 475 nm was measured. A blank test was performed and corrected in the same manner.
The tyrosinase activity inhibition rate was determined by the following mathematical formula 4. The results are shown in Table 6. The test sample was used at a sample concentration of 400 μg / mL.
<Formula 4>
Tyrosinase activity inhibition rate (%) = {1− (St−Sb) / (Ct−Cb)} × 100
St: Absorbance of the test sample solution at a wavelength of 475 nm Sb: Absorbance of the test sample solution blank at a wavelength of 475 nm Ct: Absorbance of the control solution at a wavelength of 475 nm Cb: Absorbance of the control solution blank at a wavelength of 475 nm

表６の結果から、製造例１から３の合歓の抽出物が、チロシナーゼ活性阻害作用を有することが確認できた。

From the results in Table 6, it was confirmed that the extract of Nehwa in Production Examples 1 to 3 had a tyrosinase activity inhibitory action.

(Example 5: Stem cell growth factor (SCF) mRNA expression inhibitory effect test)
Using the extract of each chewy obtained in Production Examples 1 to 3 as a test sample, the inhibitory effect on stem cell growth factor (SCF) mRNA expression was tested by the following test method.

Normal human newborn foreskin keratinocytes (NHEK) in a normal human epidermal keratinocyte long-term growth medium (EpiLife-KG2) in an 80 cm ² flask at 37 ° C. under 5% CO _2. The cells were cultured and collected by trypsinization.
Next, seeded by 40 × ¹⁰ 4 cells / 2mL / dish in 35mm petri dish (FALCON Corp.) using EpiLife-KG2, 37 ° C., and cultured overnight at 5% CO _2. After 24 hours, discard the culture solution, add 1 mL of HEPES buffer, perform UV-B irradiation (50 mJ / cm ² ), and then add 2 mL of each test sample dissolved in EpiLife-KG2 to the required concentration, The cells were cultured at 37 ° C. and 5% CO ₂ for 24 hours. After culturing, the culture medium was ^discarded, TRIzol (R) Reagent (Invitrogen Corporation; Cat.no.15596-026) total RNA was extracted by the respective amount of RNA was measured by spectrophotometer, 200 ng / [mu] L Total RNA was prepared so that
Using this total RNA as a template, the expression level of SCF (Stem Cell Factor) and GAPDH, which is an internal standard, was measured. Detection is performed by real-time One Step RT-PCR reaction using Takara One Step SYBR ^(R) RT-PCR Kit (Perfect Real Time, code No. RR046A) using a real-time PCR apparatus (Smart Cycler ^(R) , manufactured by Cepheid ⁾ . went.
The expression levels of SCF mRNA are “no UV irradiation, no test sample added”, “UV no irradiation, test sample addition”, “UV irradiation, no test sample addition”, and “UV irradiation, no test sample addition”, respectively. Based on the total RNA preparation prepared from the cultured cells, a correction value is obtained with the value of GAPDH, and when the correction value of “no UV irradiation, no test sample added” is 100, “UV irradiation, test sample” Correction values for “no addition”, “no UV irradiation, test sample addition”, and “UV irradiation, test sample addition” were calculated.
The stem cell growth factor (SCF) mRNA expression inhibition rate was determined by the following formula 5. The results are shown in Table 7. The test sample was used at a sample concentration of 10 μg / mL and 1 μg / mL.
<Formula 5>
Stem cell growth factor (SCF) mRNA expression inhibition rate (%) = {(A−B) − (A−C)} / (A−B) × 100
A: Correction value when UV irradiation is not performed and test sample is not added B: Correction value when UV irradiation is performed and test sample is not added C: Correction value when UV irradiation is applied and test sample is added

表７の結果から、製造例１から３の合歓の抽出物が、幹細胞増殖因子（ＳＣＦ）ｍＲＮＡ発現抑制作用を有することが確認できた。

From the results in Table 7, it was confirmed that the extract of Nehwa in Production Examples 1 to 3 had an action of suppressing the expression of stem cell growth factor (SCF) mRNA.

From the results of Reference Example 4 to Example 5, it was confirmed that the extract of Albizia julibrissin had at least one of a tyrosinase activity inhibitory action and a stem cell growth factor (SCF) mRNA expression inhibitory action. It has been suggested that an extract of ( Albizia julibrissin ) can be suitably used as an active ingredient of a whitening agent.

(Example 6: Matrix metalloproteinase-1 (MMP-1) activity inhibition test)
Using the extract of each cheer obtained in Production Examples 1 to 3 as a test sample, the matrix metalloproteinase-1 (MMP-1) activity inhibitory action was tested by the following test method. This test method is a partial modification of the Wunsch and Heidrich method.

In a test tube with a lid, each sample solution 50 μL, MMP-1 solution 50 μL, and Pz-peptide solution 400 μL dissolved in 0.1 mol / L Tris-HCl buffer (pH 7.1) containing 20 mmol / mL calcium chloride Were mixed and reacted at 37 ° C. for 30 minutes, and then 1 mL of a 25 mmol / L citric acid solution was added to stop the reaction. Thereafter, 5 mL of ethyl acetate was added and shaken vigorously. This was centrifuged (1,600 × g, 10 minutes), and the absorbance of the ethyl acetate layer at a wavelength of 320 nm was measured. A blank test was performed and corrected in the same manner.
As MMP-1, COLLAGENASE Type IV from Clostridium histolyticum (manufactured by Sigma) was used.
As Pz-peptide, Pz-Pro-Leu-Gly-Pro-D-Arg-OH (manufactured by BACHEM Fenchemikaline AG) was used.
And the MMP-1 activity inhibition rate was calculated | required by the following Numerical formula 6 from the obtained result. The results are shown in Table 8. The test samples were used at sample concentrations of 400 μg / mL and 100 μg / mL.
<Formula 6>
MMP-1 activity inhibition rate (%) = {1− (C−D) / (A−B)} × 100
A: Absorbance at a wavelength of 320 nm without addition of a sample solution and addition of an enzyme B: Absorbance at a wavelength of 320 nm without addition of a sample solution and addition of an enzyme C: Absorbance at a wavelength of 320 nm with addition of a sample solution and addition of an enzyme D: Addition of a sample solution Absorbance at a wavelength of 320 nm with no enzyme added

表８の結果から、製造例１から３の合歓の抽出物が、マトリックスメタロプロテアーゼ−１（ＭＭＰ−１）活性阻害作用を有することが確認できた。

From the results shown in Table 8, it was confirmed that the extract of Nehwa in Production Examples 1 to 3 had a matrix metalloproteinase-1 (MMP-1) activity inhibitory action.

(Example 7: Matrix metalloprotease-14 (MMP-14) activity inhibitory action test)
Using the extract of each chewy obtained in Production Examples 1 to 3 as a test sample, the matrix metalloproteinase-14 (MMP-14) activity inhibitory action was tested by the following test method.

In a 96-well microplate, 50 mmol / L HEPES, 10 mmol / L CaCl ₂ , 0.05 mass% Brij-35, 1 mmol / L DTNB [5,5′-dithiobis (2-nitro-benzoic acid) ] 20 μL of MMP-14 solution prepared in buffer (pH 7.5), 50 mmol / L HEPES, 10 mmol / L CaCl ₂ , 0.05 mass% Brij-35, 1 mmol / L DTNB [5,5 20 μL of each sample solution prepared with a buffer solution of “-dithiobis (2-nitro-benzoic acid)” was mixed. After reacting at 37 ° C. for 45 minutes, 10 μL of the substrate solution was added to start the reaction. The amount of 2-nitro-5-thiobenzoic acid, which is a reaction product of the mercapto group of the substrate degradation product and DTNB in the buffer solution, was measured for absorbance at a wavelength of 412 nm for 30 minutes, and based on the amount produced per minute The value of the inclination for 30 minutes was determined. A blank test was performed and corrected in the same manner.
As MMP-14, Enzyme (Human, Recombinant) From: E.M. E. coli recombinant human MMP-14 catalytic domain (Biomol) was used.
As a substrate, thiopeptide (Ac-PLG- [2-mercapto-4-methyl-pentanoyl] -LG-OC ₂ H ₅ ) (manufactured by Biomol) was used.
And the MMP-14 activity inhibition rate was calculated | required by the following Numerical formula 7 from the obtained result. The results are shown in Table 9. The test sample was used at a sample concentration of 400 μg / mL.
<Formula 7>
MMP-14 activity inhibition rate (%) = (A−B) / A × 100
A: Inclination value for 30 minutes when no sample solution is added B: Inclination value for 30 minutes when the sample solution is added

表９の結果から、製造例１から３の合歓の抽出物が、マトリックスメタロプロテアーゼ−１４（ＭＭＰ−１４）活性阻害作用を有することが確認できた。

From the results shown in Table 9, it was confirmed that the extract of the cheers from Production Examples 1 to 3 had an activity of inhibiting matrix metalloproteinase-14 (MMP-14) activity.

(Example 8: Estrogen-like action test)
The extract of each cheer obtained in Production Examples 1 to 3 was used as a test sample, and the estrogenic action was tested by the following test method.

Human breast cancer-derived cells (MCF-7) were cultured using MEM containing 10% fetal bovine serum (FBS), 1% by mass of NEAA, and 1 mmol / L sodium pyruvate, and the cells were treated with trypsin. It was collected. The collected cells were treated with activated carbon-treated 10% FBS, 1% by mass NEAA, and 1 mmol / L sodium pyruvate-containing MEM containing no phenol red (T-MEM) at 3.0 × 10 ⁴ cells / After diluting to a concentration of mL, 450 μL was seeded per well in a 48-well microplate, and cultured to fix the cells. Six hours later (day 0), 50 μL of each sample solution prepared to 10 times the final concentration with T-MEM was added to each well, and the culture was continued. On the third day, the medium was removed, 0.5 mL of the sample solution prepared to the final concentration with T-MEM was added to each well, and the culture was further continued.
Estrogen-like effects were measured using the MTT assay. After completion of the culture, the medium was removed, and MTT [3- (4,5-Dimethyl-2-thiazolyl) dissolved in MEM containing 1% by mass of NEAA and 1 mmol / L sodium pyruvate at a final concentration of 0.4 mg / mL. ) -2,5-diphenyl-2H-tetrazolium Bromide] was added to each well in an amount of 200 μL. After culturing for 2 hours, blue formazan produced in the cells was extracted with 200 μL of 2-propanol. After extraction, the absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced. As a positive control, 10 ⁻⁹ mol / L estradiol was used.
And from the obtained measurement result, the estrogen-like action (estrogen-dependent proliferation action) rate was calculated by the following formula 8. The results are shown in Table 10. The test sample was used at a sample concentration of 3.125 μg / mL.
<Formula 8>
Estrogen-like action rate (%) = (A / B) × 100
A: Absorbance when sample solution is added B: Absorbance when sample solution is not added

表１０の結果から、製造例１から３の合歓の抽出物が、エストロゲン様作用を有することが確認できた。

From the results shown in Table 10, it was confirmed that the extract of Nehwa in Production Examples 1 to 3 had an estrogen-like action.

From the results of Examples 6 to 8, the extract of Albizzia julibrissin was found to inhibit matrix metalloproteinase-1 (MMP-1) activity, matrix metalloproteinase-14 (MMP-14) activity, and estrogen-like. It was confirmed that it had at least one of the actions, and it was suggested that the extract of Albizia julibrissin can be suitably used as an active ingredient of an anti-aging agent.

( Reference Example 9: Testosterone 5α-reductase activity inhibition test)
Using the extract of each cheer obtained in Production Examples 1 to 3 as a test sample, the testosterone 5α-reductase activity inhibitory action was tested by the following test method.

In a V-bottom test tube with a weighing lid, 4.2 mg / mL testosterone 20 μL prepared with propylene glycol, 1 mg / mL NADPH-containing 5 mmol / L Tris-HCl buffer (pH 7.13) 825 μL were mixed. To this, 80 μL of a test sample prepared with ethanol, 50% ethanol, or purified water and 75 μL of a crude enzyme solution (S-9) (manufactured by Oriental Yeast) were added and mixed again, and reacted at 37 ° C. for 30 minutes. After that, 1 mL of methylene chloride was added to stop the reaction. This was centrifuged (1,600 × g, 10 minutes), and the methylene chloride layer was analyzed by gas chromatography under the following conditions. A blank test was conducted in the same manner.
In advance, 3α-androstanediol, dihydrotestosterone (DHT), and testosterone standard methylene chloride solution were similarly analyzed by gas chromatography, and the amount of compound per peak area was determined from the exact amount and peak area of these three compounds. The concentration per peak area of 3α-androstanediol, dihydrotestosterone (DHT), and testosterone after the reaction by S-9 was calculated (Formula 9). Then, according to Formula 10, the conversion rate of the test sample was obtained.
<Formula 9>
Concentration (%) = peak area of test sample × standard product concentration / standard product peak area <Equation 10>
Conversion rate (%) = (A + B) / (A + B + C)
A: Concentration of 3α-androstanediol B: Concentration of dihydrotestosterone (DHT) C: Concentration of testosterone The testosterone 5α-reductase activity inhibition rate was determined by the following formula 11 based on the conversion rate.
<Formula 11>
Testosterone 5α-reductase activity inhibition rate (%) = (1−E / D) × 100
D: Conversion rate in blank test E: Conversion rate in addition of test sample The conditions of gas chromatography are as follows.
[Conditions for gas chromatography]
-Equipment used: Shimadzu GC-7A (manufactured by Shimadzu Corporation)
Column: DB-1701 (diameter 0.53 mm × 30 m, film thickness: 1.0 μm)
Column temperature / injection temperature: 240 ° C / 300 ° C
・ Detector: FID
・ Carrier gas: Nitrogen gas

Next, the sample concentration is decreased stepwise, the testosterone 5α-reductase activity inhibition rate is measured, and the sample concentration at which the testosterone 5α-reductase activity inhibition rate becomes 50% (hereinafter referred to as “IC ₅₀ ”). (Μg / mL) was determined by interpolation. The results are shown in Table 11.

表１１の結果から、製造例１から３の合歓の抽出物が、テストステロン５α−リダクターゼ活性阻害作用を有することが確認できた。

From the results of Table 11, it was confirmed that the extract of Nehwa in Production Examples 1 to 3 had a testosterone 5α-reductase activity inhibitory action.

( Reference Example 10: Androgen receptor binding inhibitory action test)
Using the extract of each cheer obtained in Production Examples 1 to 3 as a test sample, the androgen receptor binding inhibitory action was tested by the following test method.

SC-3 cells (androgen-dependent mouse breast cancer cells) cloned from mouse spontaneous breast cancer (Shionogi cancer; SC115) were mixed with MEM containing 2% DCC-FBS and 10 ⁸ mol / L testosterone (hereinafter referred to as “MEM / 2”). The cells were collected by trypsin treatment.
The collected cells are diluted with MEM containing 2% DCC-FBS to a concentration of 1.0 × 10 ⁵ cells / ml, seeded at 100 μL per well in a 96-well plate, and overnight at 37 ° C. under 5% CO _2. Cultured.
After completion of the culture, the medium was removed, and 100 μL of a test sample dissolved in 0.5% BSA-containing Ham F12 + MEM (hereinafter sometimes referred to as “HMB”) containing 10 ⁻⁹ mol / L dihydrotestosterone (DHT). Added and incubated for 48 hours.
Thereafter, 100 μL of MTT dissolved in MEM containing 2% DCC-FBS at a final concentration of 0.4 mg / mL was added to each well, and after culturing for 2 hours, blue formazan produced in the cells was extracted with 200 μL of 2-propanol. .
After the extraction, the absorbance at 570 nm having the absorption maximum of blue formazan was measured for isopropanol containing blue formazan. At the same time, the absorbance at 650 nm was measured as turbidity, and the difference between the two absorbances was used as the amount of blue formazan produced (the absorbance in the formula for calculating the binding inhibition rate below is this corrected absorbance).
A cell cultured with only HMB was used as a blank test, and a cell cultured with HMB containing only 10 ⁻⁹ mol / L DHT was used as a positive control.
The androgen receptor binding inhibition rate (%) was determined by the following formula 12.
<Formula 12>
Androgen receptor binding inhibition rate (%) = {1− (C−D) / (A−B)} × 100
A: Absorbance when DHT is added and test sample is not added B: Absorbance when DHT is not added and test sample is not added C: Absorbance when DHT is added and test sample is added D: When DHT is not added and test sample is added Absorbance of

Next, the sample concentration is decreased stepwise and the above-mentioned androgen receptor binding inhibition rate is measured, and the sample concentration at which the androgen receptor binding inhibition rate becomes 50% (hereinafter referred to as “IC ₅₀ ”). (Μg / mL) was determined by interpolation. The results are shown in Table 12.

表１２の結果から、製造例１から３の合歓の抽出物が、アンドロゲン受容体結合阻害作用を有することが確認できた。

From the results of Table 12, it was confirmed that the extract of Nehwa in Production Examples 1 to 3 had an androgen receptor binding inhibitory action.

From the results of Reference Examples 9 to 10, it was confirmed that the extract of Albizzia julibrissin had at least one of a testosterone 5α-reductase activity inhibitory action and an androgen receptor binding inhibitory action, and Albyzzia julibrissin . It was suggested that this extract can be suitably used as an active ingredient of a hair restorer.

( Reference Example 11: Cyclic AMP phosphodiesterase activity inhibition test)
Cyclic AMP phosphodiesterase activity inhibitory action was tested by the following test method using the extract of each cheer obtained in Production Examples 1 to 3 as a test sample.

0.2 mL of 50 mmol / L Tris-HCl buffer (pH 7.5) containing 5 mmol / L magnesium chloride, 0.1 mL of 2.5 mg / mL bovine serum albumin solution and 0.1 mL of 0.1 mg / mL phosphodiesterase solution, further test 0.05 mL of the sample solution was added and pre-reacted at 37 ° C. for 5 minutes. To this, 0.05 mL of a 0.5 mg / mL cyclic AMP (cAMP) solution was added and reacted at 37 ° C. for 30 minutes. The reaction was stopped by boiling on a boiling water bath for 3 minutes, and this was centrifuged (2,260 × g, 10 minutes, 4 ° C.), and cyclic AMP, which is a reaction substrate in the supernatant, was subjected to HPLC under the following conditions. analyzed. A blank test was performed and corrected in the same manner.
[HPLC conditions]
Column: Wakosil C18-ODS 5 μm
Mobile phase: 1 mM TBAP in 25 mM KH ₂ PO ₄ : CH ₃ CN = 90: 10
Flow rate: 1.0 mL / min
Detector: 260nm
Next, the peak area (A) of the cyclic AMP standard product, the peak area (B1) of the supernatant of the reaction solution of the cyclic AMP standard product and cyclic AMP phosphodiesterase when no sample is added, and the cyclic when the sample is added The peak area (B2) of the supernatant of the reaction solution of AMP standard product and cyclic AMP phosphodiesterase was determined. From the obtained results, the cyclic AMP standard product decomposition rate (C) when no sample was added and the cyclic AMP standard product decomposition rate (D) when the sample was added were calculated from the following formulas.
Decomposition rate of standard product without addition of sample (C) (%) = (1−B1 / A) × 100
Decomposition rate of standard product at the time of sample addition (D) (%) = (1−B2 / A) × 100
Thereafter, based on the respective degradation rates (C) and (D) calculated by the above formula, the cyclic AMP phosphodiesterase inhibition rate (%) was calculated by the following formula 13. The results are shown in Table 13. The test samples were used at sample concentrations of 200 μg / mL and 50 μg / mL.
<Formula 13>
Cyclic AMP phosphodiesterase activity inhibition rate (%) = (1−D / C) × 100

表１３の結果から、製造例１から３の合歓の抽出物が、サイクリックＡＭＰホスホジエステラーゼ活性阻害作用を有することが確認できた。

From the results shown in Table 13, it was confirmed that the extract of the cheers of Production Examples 1 to 3 had a cyclic AMP phosphodiesterase activity inhibitory action.

From the result of Reference Example 11, it is confirmed that the extract of Albizza julibrissin has a cyclic AMP phosphodiesterase activity inhibitory action, and the extract of Albizzia julibrissin is suitable as an active ingredient of an antiobesity agent. It is suggested that it can be used.

(Formulation example 1)
-Emulsion-
A milky lotion was produced by a conventional method according to the following composition.
・ 50% ethanol extract of Albizzia julibrissin flower (Production Example 2) 0.10 g
・ Jojoba oil: 4.00 g
・ 1,3-Butylene glycol ・ ・ ・ 3.00g
・ Arbutin ... 3.00g
・ Polyoxyethylene cetyl ether (20E.O.) ... 2.50 g
・ Olive oil ... 2.00g
・ Squalane ... 2.00g
・ Cetanol ... 2.00g
・ Glyceryl monostearate ... 2.00g
・ Oleic acid polyoxyethylene sorbitan (20E.O.) ... 2.00g
・ Methyl paraoxybenzoate 0.15 g
・ Stearyl glycyrrhizinate ... 0.10g
・ Twilight extract ... 0.10g
・ Dipotassium glycyrrhizinate ... 0.10g
・ Ginkgo biloba extract ... 0.10g
・ Conchiolin ... 0.10g
・ Oat extract ... 0.10g
-Chamomile extract ... 0.10g
・ Fragrance ... 0.05g
・ Purified water: remainder (total 100.00 g)

(Formulation example 2)
-Lotion-
In accordance with the following composition, lotion was produced by a conventional method.
- Nemu (Albizzia julibrissin) of the flower of the water extract (Production Example 1) ··· 0.10g
・ Glycerin ... 3.00g
・ 1,3-Butylene glycol ・ ・ ・ 3.00g
・ Ascorbic acid glucoside ・ ・ ・ 2.00g
・ Oleic acid polyoxyethylene sorbitan (20E.O.) ... 2.00g
・ Methyl paraoxybenzoate 0.15 g
・ Dipotassium glycyrrhizinate ... 0.10g
・ Citric acid ... 0.10g
・ Sodium citrate: 0.10 g
・ Oil-soluble licorice extract ... 0.10g
・ Seaweed extract ... 0.10g
・ Cudin extract ... 0.10g
・ Xylobiose Mixture ... 0.05g
・ Fragrance ... 0.05g
・ Purified water: remainder (total: 100.00 g)

(Formulation example 3)
-Cream-
According to the following composition, the cream was manufactured by a conventional method.
-80% by mass ethanol extract of flower of Nebizzia julibrissin (Production Example 3) ... 0.10g
・ Squalane ... 10.00g
・ 1,3-butylene glycol ... 6.00g
・ Liquid paraffin ・ ・ ・ 5.00g
・ Salah beeswax 4.00 g
・ Cetanol ... 3.00g
・ Glyceryl monostearate ... 3.00 g
・ Lanoline ... 2.00g
・ Oleic acid polyoxyethylene sorbitan (20E.O.) ... 1.50 g
・ Methyl paraoxybenzoate ... 1.50g
・ Stearic acid: 1.00 g
・ Magnesium ascorbate phosphate ... 0.10g
・ Glycyrrhetinic acid ... 0.10g
・ Yeast extract ... 0.10g
・ Perilla extract ... 0.10g
-Linden extract ... 0.10g
・ Jiuyu extract ... 0.10g
・ Fragrance ... 0.10g
・ Purified water: remainder (total: 100.00 g)

(Formulation example 4)
−Pack−
According to the following composition, the pack was produced by a conventional method.
・ 50% ethanol extract of Albizzia julibrissin flower (Production Example 2) 0.20 g
・ Polyvinyl alcohol: 15.00g
・ Ethanol ... 10.00g
・ Propylene glycol: 7.00 g
・ Polyethylene glycol ... 3.00g
・ Sage extract ... 0.10g
・ Toki extract ... 0.10g
・ Carrot extract ... 0.10g
・ Ethyl paraoxybenzoate 0.05g
・ Fragrance ... 0.05g
・ Purified water: remainder (total: 100.00 g)

(Formulation example 5)
-Hair tonic-
A hair tonic having a hair growth action having the following composition was produced by a conventional method.
・ Pyridoxine hydrochloride ... 0.1g
・ Resorcin ・ ・ ・ 0.01g
・ D-pantothenyl alcohol ... 0.1g
・ Dipotassium glycyrrhizinate ... 0.1g
・ L-Menthol 0.05g
・ 1,3-Butylene glycol ・ ・ ・ 4.0g
・ Assembly extract ... 0.1g
・ Carrot extract ... 0.1g
・ Cudin extract ... 0.1g
・ Water extract of Albizzia julibrissin flower (Production Example 1) 0.2 g
-Fragrance-appropriate amount-Purified water-the balance (total: 100.00 g)

(Formulation example 6)
-Shampoo-
A shampoo was produced by a conventional method according to the following composition.
・ Polyoxyethylene alkyl ether sodium sulfate 30.0 g
・ Polyoxyethylene alkyl ether ammonium sulfate ... 20.0g
・ Palm oil fatty acid amidopropyl betaine ... 6.0g
・ Palm oil fatty acid modiethanolamide: 4.0 g
・ Ethylene glycol distearate ... 2.0g
・ Preservative (methyl paraoxybenzoate) ... 0.15g
-80% by weight ethanol extract of flower of Albizzia julibrissin (Production Example 3) 0.2 g
・ Muguroji extract ... 0.2g
・ Cotton extract ... 0.5g
・ Oat extract ... 0.3g
・ Rosemary extract 0.5g
・ Perfume 0.01g
・ 1,3-Butylene glycol ・ ・ ・ 3.0g
・ Purified water: remainder (total: 100.00 g)

(Formulation example 7)
-Rinse-
According to the following composition, the rinse was manufactured by the conventional method.
・ Stearyltrimethylammonium chloride 1.5g
・ Polyoxyethylene cetyl ether ... 1.0g
・ Cetyl alcohol: 2.0g
・ Octyldodecanol ・ ・ ・ 1.0g
・ Cationized cellulose: 0.5g
・ Propylene glycol ... 5.0g
・ 50% ethanol extract of Albizzia julibrissin flower (Production Example 2) 0.2 g
・ Muguroji extract ... 0.2g
・ Cotton extract ... 0.5g
・ Oat extract ... 0.3g
・ Rosemary extract 0.5g
・ Fragrance ... 3.0g
・ Purified water: remainder (total: 100.00 g)

(Formulation example 8)
-Tablet dietary supplements-
The following mixture was tableted to produce a tablet-shaped dietary supplement.
-50 % ethanol extract of Albizzia julibrissin flower (Production Example 2) 30 g
・ Powdered sugar (sucrose) 178g
・ Sorbit ・ ・ ・ 10g
・ Glycerin fatty acid ester: 12g

(Formulation example 9)
-Granular dietary supplement-
The following mixture was formed into granules to produce a granular dietary supplement.
・ 80% by mass ethanol extract of flower of Nebizzia julibrissin (Production Example 3) 20g
・ Beat oligosaccharide ... 1,000g
・ Vitamin C ... 167g
・ Stevia extract ... 10g

(Formulation example 10)
-Granular dietary supplement-
The following mixture was formed into granules to produce a granular dietary supplement.
- Nemu flower of the water extract of (Albizzia julibrissin) (Production Example 1) ··· 20g
・ Beat oligosaccharide ... 1,000g
・ Vitamin C ... 167g
・ Stevia extract ... 10g

A cosmetic comprising at least one of the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent of the present invention has an excellent antioxidant effect, anti-inflammatory effect, whitening effect, Since it has at least one of aging action, hair growth action and anti-obesity action and is excellent in safety, for example, ointment, cream, emulsion, lotion, lotion, pack, jelly, lip balm, lipstick, It can be suitably used for bathing agents, astringents, hair tonics, shampoos, rinses and the like.
In addition, foods and drinks containing at least one of the antioxidant, anti-inflammatory agent, whitening agent, anti-aging agent, hair restorer, and anti-obesity agent of the present invention have an excellent anti-oxidant action and anti-inflammatory effect even when taken orally. It has at least one of action, whitening action, anti-aging action, hair-growth action, and anti-obesity action, and is excellent in safety, so it can be suitably used for health foods, dietary supplements, etc. .

Claims (4)

Huan platelet aggregation inhibitor characterized by containing the flower Extraction of (Albizzia julibrissin). Nemu stem cell growth factor (SCF) mRNA expression inhibitor characterized by containing the Extraction of flower (Albizzia julibrissin). Nemu matrix metalloproteinase -1 (MMP-1), characterized in that it contains a flower Extraction of (Albizzia julibrissin) activity inhibitor. Nemu matrix metalloproteinase -14 (MMP-14), characterized in that it contains Extraction of (Albizzia julibrissin) Flowers activity inhibitor.