JP2001316275A - Collagen production promoter, estrogen-like agent, collagenase inhibitor, skin cosmetic, food and drink - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a collagen production promoter, an estrogen-like agent, a collagenase inhibitor, a skin cosmetic, a food and a drink capable of preventing and/or ameliorating a skin aging. SOLUTION: This collagen production promoter, this estrogen-like agent and this collagenase inhibitor comprises an extract of Alpinia speciosa, especially an extract of a leaf part and/or a stem part of Alpinia speciosa. This skin cosmetic, this food and this drink comprise the collagen production promoter, the estrogen-like agent or the collagenase inhibitor.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a collagen production promoting agent having an action of activating collagen production by fibroblasts of human skin, and an estrogen-like action having an action similar to estrogen which is a kind of female hormone. Agent,
And a collagenase inhibitor that inhibits collagenase activity involved in the reduction and denaturation of collagen. The present invention also relates to skin cosmetics having a skin aging prevention / improvement effect by adding a collagen production promoter, an estrogen-like agent or a collagenase inhibitor, and foods and drinks having a cosmetic effect.

[0002]

2. Description of the Related Art The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen which are outside these cells and support the skin structure. Is the interaction between these skin tissues keeps the water constant,
Flexibility, elasticity, etc. are ensured, and the skin is maintained in a fresh and fresh state with a sense of tension and gloss.

However, under the influence of certain external factors such as ultraviolet rays, remarkable drying of air, excessive skin washing, and aging, elastin which is a main component of the extracellular matrix is decomposed and deteriorated. Collagen causes a decrease in production and a decrease in elasticity due to crosslinking. As a result, the skin loses its moisturizing function and elasticity, and the keratin begins to peel off abnormally, so that the skin loses its tone and luster and exhibits aging symptoms such as roughness, wrinkles, dullness, and the like.

[0004] As described above, changes associated with aging of the skin, that is, wrinkles, dullness, disappearance of texture, decrease in elasticity, etc.
Reduction and denaturation of dermal matrix components such as collagen and elastin are involved. In recent years, as a factor inducing this change, particularly the involvement of a matrix protease has been pointed out. Among matrix proteases,
Collagenase, or MMP1 (matrix metalloprotease), is known as an enzyme that degrades type I and III collagens, which are the main components of the dermal matrix of the skin. It is considered to be a cause of decrease and degeneration of the skin, and a major factor such as formation of skin wrinkles. Therefore, promotion of collagen production and inhibition of collagenase activity are important in preventing and improving skin aging.

[0005] In order to prevent or improve skin aging by the mechanism described above, the most commonly used methods are natural moisturizing factors (NMF) such as sugars, amino acids, organic acids, pyrrolidone carboxylate, and collagen. And mucopolysaccharides such as hyaluronic acid, glycerin, and 1,3-butylene glycol to enhance the moisturizing property of the skin by applying a substance having a moisturizing action.

However, the moisturizer only improves the keratin condition of the epidermis, and cannot be expected to improve the tension holding mechanism in the dermis. In addition, moisturizers generally slow down the evaporation of water from the skin, and thus generally have a poor feeling of use, and may cause skin damage if used for a long time.

[0007] On the other hand, one of the causes of skin aging associated with aging is that secretion of estrogen, a kind of female hormone, is reduced. In other words, estrogen is deeply involved in the maintenance of the health of adult women, and its secretion deficiency causes various medical diseases and causes undesired skin changes such as skin hypersensitivity, decreased elasticity, and reduced moisture. Is known to be.

[0008] Therefore, a substance having the same action as estrogen is transdermally or orally administered to post-menopausal women whose estrogen secretion declines. As estrogen-like agents therefor, steroidal estrogens, non-steroidal estrogens, flavone-based compounds and the like have hitherto been used.

[0009]

DISCLOSURE OF THE INVENTION A first object of the present invention is to find a substance capable of promoting collagen production in dermal fibroblasts to prevent and / or improve skin aging, and use it as an active ingredient. An object of the present invention is to provide a collagen production promoter containing the same. A second object of the present invention is to find a substance capable of preventing and / or improving skin aging through an estrogen-like action, and to provide an estrogen-like agent containing the substance as an active ingredient. A third object of the present invention is to find a substance capable of suppressing the reduction and denaturation of collagen through a collagenase inhibitory action and preventing and / or improving skin aging, and to provide a collagenase inhibitor containing the substance as an active ingredient. It is in. A fourth object of the present invention is to promote collagen production,
An object of the present invention is to provide a skin cosmetic having an estrogen-like action or a collagenase inhibitory action and useful for preventing and / or improving skin aging. A fifth object of the present invention is to provide a food or drink having an estrogen-like action and useful for preventing and / or improving skin aging.

[0010]

Means for Solving the Problems In order to achieve the above object, the collagen production promoter, estrogen-like agent and collagenase inhibitor of the present invention are characterized by containing an extract from moon peach as an active ingredient. The skin cosmetic of the present invention is characterized by comprising the collagen production promoter of the present invention, an estrogen-like agent or a collagenase inhibitor, and the food and drink of the present invention is characterized by comprising the estrogen-like agent of the present invention. Is characterized by being blended.

[0011] In a preferred embodiment of the collagen production promoter, estrogen-like agent and collagenase inhibitor of the present invention, the extract comprises leaves and / or stems of moon peach obtained using a polar solvent as an extraction solvent. And in a more preferred embodiment, the polar solvent is water, methanol, ethanol, propanol,
1,3-butylene glycol, glycerin, propylene glycol or a mixture thereof.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.

[Collagen Production Promoter / Estrogen-like Agent / Collagenase Inhibitor] The “extract from moon peach” which is an active ingredient of the collagen production promoter, estrogen-like agent and collagenase inhibitor of the present invention includes: Extract obtained from moon peach by the extraction treatment, diluent or concentrate of the extract, dried product obtained by drying the extract,
Alternatively, any of these crude products or purified products are included.

The material used as an extraction raw material in the extraction treatment is moon peach (scientific name: Alpinia nutans or Alpinia spe
ciosa), especially the leaves and / or stems of the moon peach.

Moon peach (scientific name: Alpinia nutans or Alpi
nia speciosa) belongs to the genus Alpinia of the ginger family, and is a perennial evergreen plant distributed from southern Kyushu to India. Accordingly, moon peach is readily available from these areas. In some cases, moon peaches are cultivated for ornamental purposes in order to produce beautiful flowers in summer, but they are not used for medicinal purposes and there are no reports that they contain bioactive substances.

Moon peach used as an extraction raw material is preferably dried and ground immediately after collection. Drying may be performed in the sun or using a commonly used dryer. Moon peach may be used as an extraction raw material after pretreatment such as degreasing with a nonpolar solvent such as hexane or benzene. By performing a pretreatment such as degreasing, the extraction of moon peach with a polar solvent can be efficiently performed.

At the time of the extraction treatment, it is preferable to use a polar solvent as the extraction solvent. Collagen production promoting effect contained in moon peach (especially the leaf and / or stem of moon peach),
A component exhibiting an estrogen-like action or a collagenase inhibitory action can be easily extracted by an extraction treatment using a polar solvent as an extraction solvent.

Specific examples of suitable polar solvents include water, lower aliphatic alcohols, and hydrated lower aliphatic alcohols. These can be used alone or as a mixture of two or more of these. . Specific examples of suitable lower aliphatic alcohols include methanol, ethanol, propanol, 1,3-butylene glycol, glycerin, propylene glycol and the like.

When a mixture of two or more polar solvents is used as the extraction solvent, the mixing ratio can be adjusted appropriately. For example, when a mixed solution of water and a lower aliphatic alcohol is used, the mixing ratio of water and the lower aliphatic alcohol can be 7: 3 to 2: 8 (weight ratio).

The extraction treatment is not particularly limited as long as the soluble components contained in the moon peach can be eluted into the extraction solvent, and can be performed according to a conventional method. In the extraction treatment, it is not necessary to employ a special extraction method, and any device can be used at room temperature or under reflux.

For example, an extraction raw material is put into a treatment tank filled with an extraction solvent, and a soluble component is eluted with occasional stirring. At this time, the extraction conditions can be appropriately adjusted according to the amount of the extraction raw material and the like, but the amount of the extraction solvent is usually 5 to 15 times the weight of the extraction raw material (weight ratio), and the extraction time is usually 1 to 3 hours. The extraction temperature is usually from room temperature to 95 ° C.

After the soluble component is eluted by the extraction treatment, an extraction liquid can be obtained by filtering to remove the extraction residue. The obtained extract is diluted according to a conventional method to obtain a diluent or concentrate of the extract, a dried product of the extract, or a crude product or a purified product thereof.
Treatment such as concentration, drying, and purification may be performed. The obtained extract can be used as it is as a collagen production promoter, an estrogen-like agent or a collagenase inhibitor, but a concentrated solution or a dried product thereof is more easily used. In order to obtain a dried extract,
A carrier such as dextrin or cyclodextrin may be added to improve the hygroscopicity.

Since the extract from moon peach has a peculiar smell and taste, it can be purified for the purpose of decolorization, deodorization, etc. as long as the physiological activity is not reduced. Since it is not used in large quantities when added to cosmetics or foods and drinks, there is no practical problem even if it is unrefined.

The extract from moon peach obtained as described above has a collagen production promoting action, an estrogenic action or a collagenase inhibitory action. The extract from moon peach can be used as it is as a collagen production promoter, an estrogen-like agent or a collagenase inhibitor, but it can also be formulated and used according to a conventional method. In the case of formulation, a pharmaceutically acceptable carrier such as dextrin and cyclodextrin and other optional auxiliaries can be added to facilitate storage and handling. The extract from moon peach is powdered by formulation,
An arbitrary dosage form such as a granule or a tablet can be used.

The collagen production promoter of the present invention is absorbed percutaneously to reach fibroblasts, activates collagen production, and replenishes the dermis layer with sufficient collagen to prevent skin aging and / or Or can be improved. Further, since the collagen production promoter of the present invention has an estrogen-like action as well as a collagen production promotion action, it can act multifacetedly to prevent and improve skin aging and prevent and / or improve skin aging. .

The estrogen-like agent of the present invention can prevent and / or ameliorate aging of the skin due to impaired estrogen secretion through the estrogen-like action.

[0027] The collagenase inhibitor of the present invention suppresses collagen decrease, denaturation and the like caused by collagenase through collagenase inhibitory action, and can prevent and / or improve skin aging caused by collagen decrease and denaturation.

[Skin Cosmetic] The collagen production promoter, estrogen-like agent or collagenase inhibitor of the present invention can prevent and / or improve skin aging and provide a feeling of use when applied to the skin. Because of its excellent safety, it is suitable for blending into skin cosmetics. Any one of a collagen production promoter, an estrogen-like agent, and a collagenase inhibitor may be blended with the skin cosmetic, or two or more thereof may be blended.

[0029] Skin cosmetics which can contain the collagen production promoter, estrogen-like agent or collagenase inhibitor of the present invention are not particularly limited. Specific examples thereof include:
Examples include ointments, creams, emulsions, lotions, packs, bath preparations and the like.

The amount of the collagen production promoter, estrogen-like agent or collagenase inhibitor in the skin cosmetic of the present invention can be appropriately adjusted depending on the kind of the skin cosmetic, the physiological activity of the extract, and the like. The proper blending ratio is about 0.01 to 10% by weight in terms of a standard moon peach extract.

The skin cosmetic composition of the present invention includes various main ingredients and auxiliaries usually used in the production of skin cosmetic compositions, as long as they do not prevent collagen production promoting action, estrogen-like action or collagenase inhibitory action. Auxiliaries can be added, and the collagen production promoter, estrogen-like agent or collagenase inhibitor of the present invention is not limited to those which are the main agents for preventing and improving skin aging.

In the dermocosmetic of the present invention, examples of usable components together with the collagen production promoter, estrogen-like action or collagenase inhibitor of the present invention include glycerin, collagen, hyaluronic acid and salts thereof, and chondroitin. Humectants such as acids and salts thereof, chitin and chitosan; ultraviolet absorbers such as amyl paradimethylaminobenzoate; complex lipids such as glycerophospholipids and sphingolipids; β-carotene, oil-soluble licorice extract, lycochalcone A, baicalin, Baicalein and other substances having an active oxygen scavenging action; anti-inflammatory substances such as azulene, glycyrrhizic acid and its salts, glycyrrhetinic acid and its derivatives, zinc oxide; vitamins and their derivatives such as riboflavin, tocopherol, ascorbic acid and folic acid Oil components such as jojoba oil, lanolin, liquid paraffin, squalane, and isostearyl alcohol; surfactants such as sodium stearyl sulfate, diethanolamine cetyl sulfate, and glyceryl stearate; antioxidants such as sodium erythorbate; and preservatives such as ethyl paraben. Agent; plant extracts such as oak extract, chamomile extract, liquorice root extract, rosemary extract, marronnier extract; animal extracts such as collagen, hydrolyzed conchiolin, elastin; amino acids, cholesterols; plants Sterols; lipoproteins;
Microbial-derived components such as bifidobacterium culture, lactic acid bacteria culture, yeast extract, liquorice extract, etc .; algal extracts such as brown algae extract, red algae extract; blood circulation promoters such as γ-oryzanol; Leakage agents; fragrances; alcohols; thickeners such as carboxy polymers; titanium yellow, safflower and other coloring agents.

[Food and Drink] Since it has been confirmed that the active ingredient of the estrogen-like agent of the present invention is not digestible in the digestive tract, it can be added to any food or drink or dietary supplement. It is suitable for. In particular, the estrogen-like agent of the present invention can prevent and / or improve skin aging through an estrogen-like action, and is therefore suitable for blending into cosmetics foods and drinks. Here, “food and drink for beauty” means food and drink for the purpose of preventing and improving aging of beautiful skin or skin.

The food or drink of the present invention may be a mixture of the estrogen-like agent of the present invention in any food or drink that does not hinder its physiological activity, or the estrogen-like agent of the present invention may be mainly used. It may be a dietary supplement as an ingredient.

In preparing foods and drinks by incorporating the estrogen-like agent of the present invention, for example, saccharides such as dextrin and starch; proteins such as gelatin, soybean protein, corn protein, and the like; proteins such as alanine, glutamine, and isoleucine; Any auxiliaries such as amino acids; polysaccharides such as cellulose and gum arabic; oils and fats such as soybean oil and medium-chain fatty acid triglyceride;

The amount of the estrogen-like agent in the food or drink of the present invention may be about 1 to 1000 mg / day for the adult per day of the moon peach extract in consideration of the general intake of the food or drink to be added. It is preferable to adjust the temperature to the extent.

The food or drink to which the estrogen-like agent of the present invention can be added is not particularly limited, but specific examples thereof include:
Beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks (including concentrates and powders for preparation of these drinks); ice cream, ice sorbet, shaved ice and other ice confections; soba, udon, harusame, and gyoza no Noodles such as skins, skins of shumai, Chinese noodles, instant noodles; candy, candy, gum, chocolate, snacks, biscuits,
Confectionery such as jelly, jam, cream, baked confectionery; fish and processed livestock products such as kamaboko, ham, sausage; dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream,
Oils and fats and processed foods such as dressings; seasonings such as sauces and sauces; various forms of health, such as tablets and granules
Dietary supplements; other soups, stews, salads, side dishes, pickles, and the like.

The collagen production promoter, estrogen-like agent, collagenase inhibitor, skin cosmetics and food and drink of the present invention described above are suitably applied to humans, but their respective effects are not so high. It can be applied to animals other than humans as long as they are played.

[0039]

The present invention will be described below in further detail with reference to examples.

[Production Example 1] Moon peach (scientific name: Alpinia nutans)
Or Alpinia speciosa) crushed leaves or stems 30
0 g was poured into 2000 ml of the extraction solvent, and kept at 70 ° C. for 3 hours with gentle stirring. Thereafter, the mixture was filtered, the filtrate was concentrated at 40 ° C. under reduced pressure, and further dried with a reduced-pressure drier to obtain a moonlight extract. When the above extraction was performed using four types of extraction solvents, the yield of the extract was as shown in Table 1. When the extraction solvent is a mixture, the mixing ratios shown below are based on weight.

[Table 1] Sample No. Extraction site extraction Solvent extract yield (% by weight) 1 leaf water 28 2 leaves ethanol / water (1/1) 16 3 leaves methanol 13 4 leaves propanol / water (1 / 1) 12 5 stem water 11.6 stem ethanol / water (1/1) 7 7 stem methanol 7 8 stem propanol / water (1/1) 7

[Production Example 2] 300 g of the same crude peach leaf or stem as used in Production Example 1 was extracted with 200 m of extraction solvent.
and maintained at 80 ° C. for 3 hours with stirring.
An extract was obtained by filtration. The above extraction was carried out using four kinds of extraction solvents, and the extract having a solid content concentration of about 150 shown in Table 2 was obtained.
0 ml was obtained. When the extraction solvent is a mixture, the mixing ratios shown below are based on weight.

[Table 2] Sample No. Extraction site extraction Solvent extract concentration (% by weight) 9 leaves 1,3-butylene glycol / water (1/1) 4.2 10 leaves glycerin / water (1/1) ) 3.6 11 leaf propylene glycol / water (1/1) 3.3 12 stem 1,3-butylene glycol / water (1/1) 3.2 13 stem glycerin / water (1/1) 2.8 14 Stem Propylene glycol / water (1/1) 3.0

Test Example 1 Test for Collagen Production Acceleration Samples 1 to 14 obtained in Production Examples 1 and 2
The collagen production promoting effect was tested by the method of bster et al. (Anal. Biochem., Vol. 96, 220, 1979). In particular,
The test was performed as follows.

Human fibroblasts were seeded on a plate, and
7 ° C, 5% carbon dioxide, sample added medium (sample concentration: 2
(00 ppm or 50 ppm) for several days,
-Aminopropionitrile and [< ³ > H] -proline were added, and the cells were further cultured for 24 hours. A pepsin / acetic acid solution was added to the whole culture solution and digested at 4 ° C. for 16 hours. Then, a carrier was added to the digested solution, precipitated with 0.7 M salt, and further redissolved under neutral conditions to obtain a solution. Reprecipitated with 2M salt. After the obtained precipitate was washed with 20% ethanol, the radioactivity of the precipitate was measured.

The collagen production promotion rate was calculated assuming that the radioactivity when no sample was added was 100%. Table 3 shows the collagen production promotion rate (%) of each sample. When the sample is an extract according to Production Example 2, the “sample concentration” is a concentration in terms of solid content.

[Table 3] Sample No. Sample concentration 200 ppm Sample concentration 50 ppm 1 162.7 ± 4.3 113.0 ± 3.2 2 286.9 ± 5.3 126.0 ± 4.2 3 172.2 ± 4.7 118.5 ± 5.1 4 134.4 ± 5.1 109.2 ± 3.4 5 194.9 ± 8.0 137.1 ± 7.0 6 268.7 ± 6.3 153 0.1 ± 8.2 7 245.3 ± 9.1 126.7 ± 6.4 8 186.4 ± 8.5 118.9 ± 7.2 9 121.6 ± 4.5 102.9 ± 3. 2 10 116.8 ± 5.1 101.0 ± 3.1 11 108.2 ± 4.4 98.3 ± 3.6 12 172.5 ± 7.8 127.5 ± 5.2 13 158.5 ± 8.4 113.6 ± 7.2 14 167.7 ± 8.2 109.4 ± 6.2

From the results shown in Table 3, it was confirmed that the extract from the leaves or stems of the moon peach had an effect of promoting collagen production of fibroblasts. In addition, the intensity of the collagen production promoting effect of the moon peach extract varies depending on the concentration of the moon peach extract, and the intensity of the collagen production promoting effect can be adjusted by adjusting the concentration of the moon peach extract. Was confirmed.

[Test Example 2] Test of estrogen-like action [0049] For samples 1 to 14 obtained in Production Examples 1 and 2, the effect of estrogen-dependent cell proliferation on Th was examined.
Omas et al. (In Vitro Cell. Dev. Biol. 28A, 5
95-602, 1992). Specifically, the test was performed as follows.

MCF-7 cells derived from human breast cancer were treated with 75c
m ³ flask and cultured until confluent like, collect the MCF-7 cells by trypsinization, 10%
It was adjusted to 3 × 10 ⁴ cells / ml using a minimal nutrient medium containing activated carbon-treated FBS, 1% NEAA and 1 mM sodium pyruvate and no phenol red.

0.9 ml of each of the obtained MCF-7 cells was seeded on a 24-well microplate, and cultured at 37 ° C. under 5% carbon dioxide to fix the cells. Six hours later (day 0), 100 μl of a sample solution adjusted to 10 times the final concentration in a minimal nutrient medium containing no phenol red
(Sample concentration: 125 ppm or 62.5 ppm) was added to the microplate, and the culture was continued.

On the sixth day from the start of the culture, in order to measure the number of MCF-7 cells, the minimal nutrient medium was replaced with MTT medium, and blue formazan produced in the cells by adding 2-propanol was used. Extracted and measured absorbance at 570 nm. At the same time, the absorbance at 650 nm was measured as turbidity, and the difference between the two was taken as the amount of blue formazan. As a positive control, 0.02 ppm ethinylestradiol was used. The strength of the estrogen-like action (estrogen-dependent growth action) was calculated assuming that the absorbance when no sample was added was 100%. Table 4 shows the strength of the estrogen-like action (estrogen-dependent growth action) of each sample.

[Table 4] Sample No. Sample concentration 12.5 ppm Sample concentration 6.25 ppm 1 137.4 ± 3.3 130.1 ± 1.4 2 147.3 ± 2.6 133.4 ± 1.3 3 139.8 ± 2.9 127.8 ± 1.8 4 130.5 ± 3.4 123.9 ± 2.0 5 138.7 ± 5.2 122.3 ± 4.8 6 145.2 ± 3.4 122.4 ± 3.5 7 141.4 ± 4.2 118.9 ± 2.4 8 131.5 ± 3.7 116.1 ± 3.2 9121.8 ± 2.9 111. 0 ± 1.2 10 124.2 ± 2.8 105.1 ± 1.5 11 122.5 ± 3.5 107.4 ± 1.1 12 128.2 ± 2.9 114.3 ± 3.4 13 125.1 ± 3.0 109.4 ± 2.8 14 118.7 ± 4.5 107.8 ± 3.5

From the results shown in Table 4, it was confirmed that the extract from the leaves or stems of the moon peach had an estrogenic effect. In addition, it was confirmed that the intensity of the estrogenic effect of the moon peach extract changes depending on the concentration of the moon peach extract, and that the intensity of the estrogenic effect can be adjusted by adjusting the concentration of the moon peach extract. Was done.

Test Example 3 Test for Collagenase Inhibition Effect Samples 1 to 3 and 5 to 7 obtained in Production Example 1 were
Collagenase inhibitory activity was tested by the following method.

(1) 0.1 containing 20 mM calcium chloride
50 μl of a sample solution obtained by dissolving a sample in M Tris-HCl buffer (pH 7.1), a collagenase solution (concentration: 0.1 g /
ml) and substrate solution (concentration: 0.5M) 40
0 μl was mixed and incubated at 37 ° C. for 30 minutes. Next, 1 ml of a 25 mM citric acid solution was added to stop the reaction, and the mixture was extracted with 5 ml of ethyl acetate. The obtained extract was measured for absorbance at a wavelength of 320 nm (control solution: ethyl acetate). Hereinafter, this absorbance is referred to as “absorbance at the time of addition of the sample solution / addition of the enzyme”.

(2) The absorbance was measured in the same manner as in (1) except that a Tris-HCl buffer was used instead of the collagenase solution. Hereinafter, this absorbance is referred to as “absorbance when the sample solution is added / the enzyme is not added”.

(3) Absorbance was measured in the same manner as in (1) above, except that an equal amount of Tris-HCl buffer was used instead of the sample solution. Hereinafter, this absorbance is referred to as “absorbance when no sample is added / enzyme is added”.

(4) The absorbance was measured in the same manner as in (1) above, except that a Tris-HCl buffer was used instead of the collagenase solution, and an equivalent amount of the Tris-HCl buffer was used instead of the sample solution. did. Hereinafter, this absorbance is referred to as “absorbance when no sample is added and no enzyme is added”.

(5) From the above measurement results, the collagenase inhibition rate (%) was calculated using the following equation.

[0061]

[Formula 1] Collagenase inhibition rate (%) = [1- (AB)
/ (CD)] x 100

In the above formula, “A” represents the absorbance when the sample solution is added and the enzyme is added, “B” is the absorbance when the sample solution is added and the enzyme is not added, and “C” is the absorbance when the sample solution is added and the enzyme is not added. The absorbance, “D”, indicates the absorbance when no sample was added and no enzyme was added.

The concentration of the sample solution was gradually reduced to measure the above-mentioned inhibition rate, and the concentration (ppm) of the sample solution that inhibited collagenase activity by 50% was determined by an interpolation method. 50% collagenase activity for samples 1-3 and 5-7
The inhibitory concentrations (ppm) are shown in Table 5.

[Table 5] Sample No. 50% inhibitory concentration (ppm) 1 105.8 2 52.1 3 58.9 5 116.3 6 102.9 7 112.6

From the results shown in Table 5, it was confirmed that the extract from the leaves or stems of the moon peach had a collagenase inhibitory action. In addition, the strength of the collagenase inhibitory action of the moon peach extract changes depending on the concentration of the moon peach extract, and it was confirmed that the strength of the collagenase inhibitory action could be adjusted by adjusting the concentration of the moon peach extract. Was done.

[Test Example 4] Test for improvement of skin roughness (prevention and improvement of skin aging) Emulsion containing 50% ethanol extract of moon leaves (sample 2) obtained in Production Example 1 (hereinafter referred to as "Example") Emulsion ") was prepared according to a conventional method. The composition of the emulsion of the example is shown below.

Moon Peach Extract (Sample 2 of Production Example 1) 1.0 g Cetyl alcohol 0.5 g Beeswax 2.0 g Polyoxyethylene sorbitan oleate (10E.0) 1.0 g Glyceryl monostearate 1.0 g Hyaluron Acid 0.1 g Propylene glycol 5.0 g Ethanol 3.0 g Methyl parahydroxybenzoate 0.3 g Fragrance 0.03 g Purified water Remainder (total amount is 100 ml)

The following evaluation tests were carried out on the milk of the example and the milk of the comparative example having the same composition as the milk of the example except that the extract of the moon peach was not contained. Subjects: From a large number of women aged 22 to 43 years, the kerfs and kerfs have disappeared and a wide range of keratin has been turned up (the score shown in Table 6 is 1), or the kerfs and kerfs are unclear and the keratin is Twenty subjects who were partially turned up (the score shown in Table 6 was 2) and were determined to have rough skin were selected as subjects. Application test: To each subject, the emulsion of the example was applied to the right half of the face and the emulsion of the comparative example was applied to the left half once each morning and evening for 30 days.

[Judgment 1: Skin Roughness Improving Effect] After completion of the coating test, a replica of the face was taken by a replica method using Silflo (manufactured by FLEXICL DEVELOPMENTS LTD). Observation was made and the skin condition was determined according to the evaluation criteria shown in Table 6. Table 7 shows the determination results.

[0070] [Table 6] disappears commentary points Evaluation 1 Kawamizo-skin hill, a wide range of horny is turned over. (Rough skin condition) 2 Skin grooves / skins are unclear. The keratin is partially turned up. (Rough skin condition) 3 Skin furrow / skin is observed but it is flat. (Normal skin) 4 Skin groove / skin is clear. (Relatively beautiful skin) 5. The skin sulcus / skin is extremely clear and well-organized. (Beautiful skin)

[Table 7] Example before start of scoring test Example of emulsion application part Comparative example Emulsion application part 1 12 0 8 8 2 8 8 3 0 7 7 3 4 0 8 1 50 people 5 people 0 people

As shown in Table 7, the area to which the emulsion of the example was applied was significantly improved in skin roughness (aging of the skin) as compared with the area to which the emulsion of the comparative example was applied.

[Judgment 2: Sensory Evaluation] All subjects were asked about the superiority and inferiority of the emulsion of the example and the emulsion of the comparative example with respect to the feeling of use and the effect on the skin. Table 8 shows the results of counting the answers.

[Table 8] Evaluation items Example Examples Good emulsions Comparative examples Good emulsions Good and less familiarity to skin 13 5 2 Moist 19 19 0 1 Skin growth 15 3 2 Name Satisfaction of skin roughness improvement 17 2 1 1 Satisfaction of skin color improvement 14 4 2 Improve the number and depth of wrinkles 19 1 0

From the results shown in Table 8, the same effects as in the above-mentioned judgment 1 and excellent feeling in use were confirmed also by the sensory evaluation. From the results of determinations 1 and 2, the skin cosmetic containing the moon peach extract has an effect of preventing and improving skin aging (an effect of improving skin roughness) and is excellent in use feeling and safety when applied to the skin. It was confirmed that.

Example 1 An emulsion having the following composition was prepared by a conventional method. Jojoba oil 4 g Olive oil 2 g Squalane 2 g Cetanol 2 g Glyceryl monostearate 2 g Polyoxyethylene cetyl ether (20E.0) 2.5 g Polyoxyethylene sorbitan oleate (20E.0) 2 g 1,3-butylene glycol 3 g Paraoxybenzoic acid Methyl 0.15 g Fragrance 0.05 g Moon peach extract (Sample 3 of Production Example 1) 0.1 g Moon peach extract (Sample 9 of Production Example 2) 1 g Purified water Remainder (total amount is 100 g)

Example 2 A lotion having the following composition was prepared by a conventional method. Glycerin 3g 1,3-butylene glycol 3g Polyoxyethylene sorbitan oleate (20E.0) 0.5g Methyl parahydroxybenzoate 0.15g Citric acid 0.1g Sodium citrate 0.1g Flavor 0.05g Moon peach extract ( Sample 1 of Production Example 1) 0.2 g moon peach extract (Sample 9 of Production Example 2) 2 g Purified water The remainder (total amount is 100 g)

Example 3 A cream having the following composition was produced by a conventional method. Liquid paraffin 5 g Salami beeswax 4 g Cetanol 3 g Squalane 10 g Lanolin 2 g Stearic acid 1 g Polyoxyethylene sorbitan oleate (20E.0) 1.5 g Glyceryl monostearate 3 g 1,3-butylene glycol 6 g Methyl paraoxybenzoate 1.5 g Flavor 0 0.1 g moon peach extract (sample 3 of Production Example 1) 0.1 g moon peach extract (sample 9 of production example 2) 1 g purified water remaining (total amount is 100 g)

Example 4 A pack having the following composition was produced by a conventional method. Polyvinyl alcohol 15 g Polyethylene glycol 3 g Propylene glycol 7 g Ethanol 10 g Ethyl parahydroxybenzoate 0.05 g Perfume 0.05 g Moon peach extract (Sample 9 of Production Example 2) 5 g Purified water The remainder (total amount is 100 g)

Example 5 The following mixture was tableted to obtain a dietary supplement. Moon peach extract (sample 2 of Production Example 1) 50 parts by weight Powdered sugar (sucrose) 188 parts by weight Glycerin fatty acid ester 12 parts by weight

Example 6 The following mixture was formed into granules to obtain dietary supplements. Moon peach extract (Sample 1 of Production Example 1) 34 parts by weight Beet oligosaccharide 1000 parts by weight Vitamin C 167 parts by weight Stevia extract 10 parts by weight

[0082]

According to the present invention, a collagen production promoter,
Estrogenic agents and collagenase inhibitors are provided. The collagen production promoter, estrogen-like agent and collagenase inhibitor of the present invention are useful for preventing and / or improving skin aging. In addition, the present invention provides a skin cosmetic containing a collagen production promoter, an estrogen-like agent or a collagenase inhibitor. The skin cosmetic of the present invention has a collagen production promoting action, an estrogen-like action or a collagenase inhibitory action, and is excellent in use feeling and safety when applied to the skin, so that it prevents skin aging and Useful for improving. Further, according to the present invention,
A food or drink containing an estrogen-like agent is provided. The food or drink of the present invention is useful for preventing and / or improving skin aging.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 7/48 A61P 5/30 A61P 5/30 43/00 105 43/00 105 111 111 C12N 9/99 C12N 9/99 A23L 2/00 F (72) Inventor Naoko Kishida 14703-10 Mukotocho, Onomichi-shi, Hiroshima Maruzen Pharmaceutical Co., Ltd. F-term (reference) 4B017 LC03 LG15 LP01 4B018 LE01 MD61 ME14 MF01 4C083 AA082 AA111 AA112 AA122 AC022 AC072 AC102 AC122 AC182 AC242 AC302 AC422 AC442 AC482 AD112 AD332 CC05 CC07 DD27 EE12 EE13 4C088 AB81 AC05 CA05 CA06 CA08 MA63 ZA89

Claims (11)

[Claims] 1. A collagen production promoter comprising an extract from moon peach as an active ingredient. 2. The collagen production promoter according to claim 1, wherein the extract is an extract from the leaves and / or stems of moon peach obtained using a polar solvent as an extraction solvent. . 3. The polar solvent is water, methanol, ethanol, propanol, 1,3-butylene glycol,
The collagen production promoter according to claim 2, which is glycerin, propylene glycol or a mixture thereof. 4. An estrogenic agent comprising an extract from moon peach as an active ingredient. 5. The estrogen-like agent according to claim 4, wherein the extract is an extract from leaves and / or stems of moon peach obtained using a polar solvent as an extraction solvent. . 6. The polar solvent is water, methanol, ethanol, propanol, 1,3-butylene glycol,
The agent according to claim 5, wherein the agent is glycerin, propylene glycol or a mixture thereof. 7. A collagenase inhibitor comprising an extract from moon peach as an active ingredient. 8. The collagenase inhibitor according to claim 7, wherein the extract is an extract from the leaves and / or stem of a moon peach obtained using a polar solvent as an extraction solvent. 9. The method according to claim 9, wherein the polar solvent is water, methanol, ethanol, propanol, 1,3-butylene glycol,
9. The collagenase inhibitor according to claim 8, which is glycerin, propylene glycol or a mixture thereof. 10. The collagen production promoter according to any one of claims 1 to 3, the estrogen-like agent according to any one of claims 4 to 6, or the collagenase according to any one of claims 7 to 9. Skin cosmetics containing an inhibitor. (11) A food or drink comprising the estrogen-like agent according to any one of (4) to (6).