KR102323101B1 - Composition for improving hair and scalp condition coprising extract of hydrangea serrata - Google Patents

Abstract

The present specification relates to a composition for improving hair and scalp conditions comprising a cornflower extract. More specifically, it relates to a composition for improving the condition of hair and scalp, which has the effect of alleviating the itchiness of the scalp and the generation of dandruff by including the extract of cornflower hydrangea, and exhibits the effect of preventing hair loss and promoting hair growth. Therefore, the composition for improving hair and scalp conditions of the present specification may be used as a pharmaceutical, food or cosmetic composition.

Description

Composition for improving the condition of hair and scalp, including hydrangea extract

The present specification relates to a composition for improving hair and scalp conditions comprising a cornflower extract.

In the modern society that values beauty, hair dyes and perms are used to satisfy the desire to express individuality. In addition, various harmful substances from the outside continuously contaminate the scalp and hair. In the case of some sensitive scalps, chemical treatments such as dyeing and perming may cause irritation and inflammation such as itching or stinging of the scalp. These stimuli and inflammation lead to environmental hair loss, and various studies are being actively conducted to prevent hair loss that causes serious mental pain and promote hair growth.

Therefore, it is urgent to develop a composition having the effect of improving the scalp condition due to the use of various hair dyes, perming agents, and the like, external contaminants, as well as preventing hair loss and promoting hair growth.

Recently, in order to solve the above problems, a lot of compositions for external application for skin such as cosmetics using natural products have been developed. Accordingly, the present inventors have studied the possibility of application as external preparations for skin from plants that are not widely known so far.

US 10-0960054 B1

The inventors of the present specification have studied a composition that can provide dandruff suppression and scalp itch improvement and at the same time preventing hair loss and hair growth effect when used on hair and scalp by solving the above problems. , found that this problem can be solved and completed the present invention.

Accordingly, the problem to be solved by the present specification is to provide a composition capable of improving hair and scalp conditions.

In order to solve the above object, the present specification provides a composition for improving hair and scalp conditions comprising an extract of cornflower hydrangea as an active ingredient.

In one aspect of the present specification, the composition may be a composition for inhibiting dandruff or improving scalp itchiness and preventing hair loss and promoting hair growth.

According to the composition for improving hair and scalp conditions comprising the extract of cornflower hydrangea of the present specification, it is possible to provide an effect of improving scalp itching and preventing dandruff, preventing hair loss and promoting hair growth, and compositions for external application for skin such as cosmetic compositions and pharmaceutical compositions can be used as

The present specification, in one aspect, may relate to a composition comprising an extract of hydrangea ( Hydrangea serrata ) as an active ingredient.

In one aspect of the present specification, the composition may be a pharmaceutical, food, or cosmetic composition.

In one aspect of the present specification, the composition may be a composition for improving hair or scalp condition.

In one aspect of the present specification, improvement of hair or scalp condition may include: alleviating scalp irritation; suppression of scalp itchiness; to improve, prevent or treat dandruff; to improve, prevent or treat hair loss; Or it may be one that promotes hair growth.

In one aspect of the present specification, the hydrangea may be one or more selected from the group consisting of whole plants, leaves, stems, branches, bark, flowers, seeds and fruits of the woody hydrangea.

In one aspect of the present specification, the corn hydrangea extract may be extracted by mixing leaves, fruits and bark in a weight ratio of 7:2:1. The weight ratio may be a weight ratio of 1 to 10:1 to 3:1, or a weight ratio of 6 to 8: 1.5 to 2.5:1.

In one aspect of the present specification, the extract of cornflower hydrangea may be one or more extracts selected from the group consisting of water, an organic solvent, and mixtures thereof. The organic solvent may be at least one selected from the group consisting of ethanol, butylene glycol, and propylene glycol.

In one aspect of the present specification, the composition may be a composition for external application to the skin.

Arithmetic station (Hydrangea serrata) herein, the genus Hydrangea (Hydrangea) plant, genus Hydrangea macrophylla, Hydrangea and belongs to the (Hydrangeaceae), consists of a neck nature shrub of about 23 species. It is native to the Western Hemisphere and Eastern Asia. Hydrangea plants growing in Korea include hydrangea ( H. serrata ) and hydrangea. Hydrangeas and hydrangeas grow in mountains or fields, and the varieties planted in the garden are hydrangeas and their varieties. Mountain hydrangea can be found all over Korea, but water hydrangea only grows on Ulleungdo and Jeju Island. In addition, tree hydrangeas and American hydrangeas are imported from abroad and planted for landscaping in gardens.

Hydrangea serrata referred to herein is, Hydrangea Serrata for . acuminata (Siebold & Zucc) Wilson), Hydrangea serrata for Hydrangea serrata for. buergeri Nakai., Hydrangea serrata for Koreana tea. Lee ( Hydrangea serrata for. coreana T. Lee) and Hydrangea serrata for. fertilis Nakai.

In the present specification, hydrangea Serrata for acuminata (S. et z.) Wilson (Hydrangea Serrata for. acuminata (Siebold & Zucc) Wilson), hydrangea (Lee Chang-bok, Korean botanical encyclopedia, 1984) or flower landscape It is also called soup (Songjiok, 1980, Korea Plant Resources), and is also called hairy hydrangea. It is a dicotyledonous broad-leaved shrub in the family Rosaceae. It grows wild at an altitude of 200-1,400m in the south central part of Korea, and is also distributed in Japan and Taiwan. It usually grows up to 1m, prospers by giving out many stems under the stem, and there are fine hairs on the annual branches. The root has fine roots and normal roots, and the leaves are opposite, oval or egg-shaped, with a long pointed tip like a tail, and the lower part is round or acute, 5-15cm long and 2-10cm wide, with sharp saw teeth on the edge. There are hairs on the side veins and the posterior veins. Flowers have large flat inflorescences at the end of branches grown in the current year in July-August, hairy, and the non-sexual flowers around them are 2-3cm in diameter, and sepals are like petals, with 3-5 pieces, white, reddish-walled or wall-colored. Bisexual flowers have small sepals and 5 each together with petals. Stamens are 5 and the length is 3~4mm together with the style, and there is 1 pistil, and the style is 3~4. The fruits ripen in September-October and are small, large honey egg-shaped capsules.

In the present specification, Hydrangea serrata for. buergeri Nakai. is also called Flowering Landscape Hydrangea (Lee Chang-bok, Korean Botanical Encyclopedia, 1984) or Goryeo Hydrangea (Songjiok, 1980, Korea Plant Resources). . It is distributed in the south central part of Korea, including Jeju Island, Japan, and Taiwan. The growing temperature is 16~30℃, and it grows well in the waterside or valleys with a lot of soil moisture. Grows up to 1 m in height. The leaves are opposite, long oval, and pointed at the tip. The length of the leaf is 5~15cm and the width is 2~10cm, with saw teeth on the edge and hairs on the veins. The flowers are white or blue-purple, and hang in coaxial inflorescences at the end of branches in July-August. Nearby neuter flowers have 3 to 5 sepals, serrated, and look like petals. A bisexual flower hangs in the center, and there are 5 petals and 5 sepals each. The fruit is a capsule (朔果) that ripens in October and has fine roots.

In the present specification, hydrange Serrata for Koreana tea. Lee ( Hydrangea serrata for. coreana T. Lee), also called cotyledonous hydrangea (Lee Chang-bok, Korean Botanical Encyclopedia, 1984) or long-leaved mountain hydrangea (Songjiok, Korea Plant Resources, 1980), is a deciduous broad-leaved shrub of the dicotyledonous family Rosaceae. . Distributed in central and south of Korea, it usually grows up to 1 m, grows by giving out many stems under the stem, and has fine hairs on annual branches. The root has fine roots and normal roots, and the leaves are opposite, oval or egg-shaped, with a long pointed tip like a tail, and the lower part is round or acute, 5-15cm long and 2-10cm wide, with sharp saw teeth on the edge. There are hairs on the side veins and the back veins, and the leaves are especially thick. Flowering period is July-August, and numerous bisexual flowers form flat inflorescences together with decorative flowers at the ends of branches grown in the current year. Decorative flowers have sepals in the shape of petals, 3 to 5 pieces, and are blue or white. Bisexual flowers have small sepals and 5 each together with petals. Stamens are 10, longer than petals. There is 1 pistil and 3 to 4 styles. The fruits ripen in autumn and are egg-shaped capsules.

In the present specification, hydrangea serrata for fertilis Nakai (Hydrangea serrata for. fertilis Nakai.) is called Tamna hydrangea (Songjiok, Korea Plant Resources, 1980) or island hydrangea, and is a deciduous shrub of the dicotyledonous Rosaceae family. It is distributed in Jeju Island, Korea, and grows at the foot of a mountain or at the edge of a forest. It grows up to about 1m in height, grows by producing many stems from the bottom, and has fine hairs on annual branches. Leaves are opposite, oval or egg-shaped, with a long pointed tip like a tail, with a round or acute bottom at the bottom, 5-15 cm long and 2-10 cm wide, with sharp saw teeth, and hairs on the side veins on the surface and on the veins on the back side. . Flowers bloom in July-August, and large flat inflorescences hang at the tip of the branch grown in the current year, and there are hairs. A large number of bisexual flowers form a large flat inflorescence along with asexual flowers with a diameter of 2-3 cm. Asexual flowers have sepals in the form of petals, 3 to 5, and are blue or purple. Bisexual flowers have small sepals and 5 each together with petals. Stamens are 10, longer than petals, and pistil is 1, and style is 3-4. The fruits are egg-shaped and ripen in autumn.

The hydrangea used in this specification was sampled from plants planted as ornamental trees at the Osulloc Tea Museum in Seogwang Dawon, Jeju.

The method for preparing the extract of cornflower hydrangea used herein is not particularly limited, and in the present specification, the extract of cornflower hydrangea is a leachate obtained by leaching and discharging from cornflower hydrangea, a concentrate obtained by concentrating part or all of the leachate again, and again the It includes an extract prepared by drying the concentrate, or an oil obtained by distillation or containing a chemical substance that exerts the main effect contained in the extract.

Cornflower hydrangea as used herein may be included in the form of an extract, a pulverized product of the crude drug itself, or a dry pulverized product of the crude drug, but is not limited thereto. In addition, the corn hydrangea used in the present specification is not limited in the method of obtaining it, and it can be cultivated and used or can be purchased and used on the market, and part or all of the above-ground part or root part of wood can be used, preferably all of the above-ground part can be used Specifically, for the corn hydrangea, at least one selected from the group consisting of whole plants, leaves, stems, branches, bark, flowers, seeds and fruits of the woody hydrangea may be used.

In the present specification, "extract" includes all substances obtained by extracting the components of natural products, regardless of the extraction method, extraction solvent, extracted components, or the form of extracts It is a broad concept that includes all substances obtainable by processing or processing by a method.

In the present specification, "corn hydrangea extract" includes all substances obtained by extracting the components of corn hydrangea, regardless of the extraction method, extraction solvent, extracted component or the form of the extract, and in the process of extracting the component, heat, acid (acid), base (base), includes a substance obtained through an extraction method including a process of enzyme treatment, etc., and can also be obtained by extracting the material obtained by extracting the components of cornflower hydrangea and processing or treating it by another method It is a broad concept that includes all substances that exist.

In one aspect of the present specification, the corn hydrangea extract comprises the steps of washing and drying corn hydrangea; solvent extraction of dried cornflower; and filtering the extracted cornflower hydrangea. In the above, the solvent includes at least one selected from the group consisting of water, an organic solvent, and a mixture of water and an organic solvent. In the above, water includes distilled water or purified water, and the organic solvent is selected from the group consisting of alcohol, acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone, and chloroform, for example, _{C 1} to C _{5 lower alcohol.} One or more include, but are not limited to.

The cornflower extract may include a cornflower-C ₁ ~C ₆ alcohol extract, and specifically, the alcohol may be methanol or ethanol.

In the present specification, the cornflower extract may be a crude extract of a solvent selected from the group consisting _{of water, C 1} -C _{6 alcohol, and a combination thereof.} The C ₁ -C ₆ alcohol may be specifically methanol. When extracting cornflower hydrangea with a solvent, it is preferable to extract by adding a solvent corresponding to about 5 to 15 times that of cornflower hydrangea, and specifically, it is preferable to extract by adding about 10 times the solvent, but is not limited thereto. Heat extraction, cold extraction, reflux cooling extraction, ultrasonic extraction, etc. may be used for the extraction, and there is no limitation as long as the extraction method is obvious to those skilled in the art. The extraction may be performed at room temperature, but for more efficient extraction, it may be performed under a heating condition, preferably at a temperature of about 40 to 100°C, more preferably at a temperature of about 80°C, but limited thereto it is not The extraction time is preferably about 2 to 4 hours, more preferably about 3 hours, but is not limited thereto, and may vary depending on conditions such as extraction solvent and extraction temperature. The extraction may be performed at least once or several times in order to obtain a larger amount of the active ingredient, preferably 1 to 5 times, more preferably 3 times consecutive extraction, and the combined extract may be used.

In the present specification, the extract of cornflower hydrangea may include a crude extract of cornflower hydrangea as described above, and may be included as a soluble fraction of an organic solvent obtained by further extracting the crude extract with an organic solvent having low polarity. The organic solvent may be hexane, methylene chloride, ethyl acetate, n-butanol, or the like, but is not limited thereto. The extract extracted by the above method or a soluble fraction of the extract may be used as it is, but it may be used in the form of an extract by concentration after filtration, or in the form of a freeze-dried product after concentration and freeze-drying.

As used herein, the term “hair” refers to body hair and hair, and may also be described as hair. The hair includes both human and animal hair.

Corneous hydrangea extract of the present specification is chopped and dried; Adding water, an organic solvent, or a combination thereof to the dried cornflower hydrangea and extracting under reflux; filtration with a filter cloth after depositing the extract; centrifuging the filtrate to separate the residue and the filtrate; It can be obtained by a method comprising; concentrating the separated filtrate under reduced pressure to obtain an extract of cornflower hydrangea.

In one aspect of the present specification, the cornflower extract is composed of purified water, ethanol, butylene glycol, and propylene glycol so that the cornflower hydrangea concentrate concentrated under reduced pressure according to the above method is included in 5% by weight based on the total weight of the extract. It may be prepared by dispersing/dissolving using one or more solvents selected from the group.

In addition, in the extraction process of the extract of cornflower hydrangea of the present specification, for example, water or an organic solvent is added to chopped and dried hydrangea, extracted under reflux and deposited, and then the residue and the filtrate are separated through filter cloth filtration and centrifugation, The separated filtrate can be concentrated under reduced pressure to obtain a cornflower extract. The organic solvent usable herein may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform, or a mixed solvent of these organic solvents and water, and preferably 100% water, 70% ethanol in consideration of the safety of the raw material. Or butylene glycol (Butylene Glycol) is used. At this time, the extraction temperature is preferably 18 ~ 80 ℃ in the case of water, 18 ~ 40 ℃ in the case of 70% ethanol and butylene glycol, and the extraction time is preferably 6 ~ 168 hours. If the extraction temperature and extraction time are out of the range, extraction efficiency may decrease or a change in components may occur. After obtaining the extract using the solvent in the above, it is possible to obtain a liquid product by cooling, heating, and filtration at room temperature by a conventional method known in the art, or additionally evaporating the solvent, spray drying or freeze drying. In addition, during freeze-drying, freeze-drying may be performed at -50 to -70°C for 3 to 4 days.

In the present specification, the cornflower extract may be included in an amount of 0.00005 to 50% by weight based on the total weight of the composition. More preferably, 0.1 to 1% by weight or 0.5 to 10% by weight may be used. If the extract of cornflower hydrangea is less than 0.00005% by weight, the effect is insignificant, and if it is more than 50% by weight, stability or formulation problems may occur. Specifically, the cornflower extract is 0.0001 wt% or more, 0.0005 wt% or more, 0.001 wt% or more, 0.01 wt% or more, 0.1 wt% or more, 0.3 wt% or more, 0.5 wt% or more, 0.8 wt% or more, based on the total weight of the composition. , 1 wt% or more, 3 wt% or more, 5 wt% or more, 7 wt% or more, 10 wt% or more, 15 wt% or more, 20 wt% or more, 25 wt% or more, 30 wt% or more, 35 wt% or more , or 40% by weight or more, and may be 60% by weight or less or 50% by weight or less.

In one aspect of the present specification, the concentration of the cornflower extract may be 0.01ug/mL to 100ug/mL. In addition, the concentration of the cornflower extract is 0.01ug/mL or more, 0.1ug/mL or more, 0.3ug/mL or more, 0.4ug/mL or more, 0.5ug/mL or more, 0.6ug/mL or more, 0.7ug/mL or more, It may be 1ug/mL or more, 5ug/mL or more, 10ug/mL or more, 100ug/mL or more, or 1000ug/mL or more, and may be 100mg/mL or less or 10mg/mL or less.

The pharmaceutical composition according to the present specification may be in various oral or parenteral formulations. In the case of formulation, it is prepared using diluents or excipients, such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, soft or hard capsules, etc., and these solid preparations include at least one excipient in one or more compounds, for example, starch, calcium carbonate, sucrose. Or it is prepared by mixing lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, internal solutions, emulsions, syrups, etc. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like can be used.

The pharmaceutical dosage form of the composition of the present specification may be used in the form of a pharmaceutically acceptable salt thereof, and may be used alone or in combination with other pharmaceutically active compounds as well as in an appropriate group. The salt is not particularly limited as long as it is pharmaceutically acceptable, and for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid , benzenesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid, and the like can be used.

The composition of the present specification may be administered parenterally or orally as desired, and may be administered in 1 to several divided doses to be administered in an amount of 0.1 to 500 mg, preferably 1 to 100 mg per 1 kg of body weight per day. have. The dosage for a specific patient may vary depending on the patient's weight, age, sex, health status, diet, administration time, administration method, excretion rate, severity of disease, and the like.

The pharmaceutical composition according to the present specification may be prepared according to a conventional method according to a conventional method; oral dosage forms such as powders, granules, tablets, soft or hard capsules, suspensions, emulsions, syrups, aerosols, external preparations for skin such as ointments, creams, suppositories, and injections And it may be formulated and used in any form suitable for pharmaceutical preparations, including sterile injection solutions.

The composition according to the present specification may be administered to mammals such as rats, mice, livestock, and humans by various routes such as parenteral and oral administration, and all modes of administration can be expected, for example, oral, rectal Or it may be administered by intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

In one aspect of the present specification, the food composition may be a health functional food composition.

The formulation of the food composition according to the present specification is not particularly limited, but may be formulated as, for example, tablets, granules, powders, liquids such as drinks, caramel, gels, bars, and the like. In addition to the active ingredient, the food composition of each dosage form can be appropriately selected by those skilled in the art without difficulty depending on the dosage form or purpose of use in addition to the active ingredient, and a synergistic effect may occur when applied simultaneously with other raw materials.

In the food composition according to the present specification, the determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage thereof is, for example, 0.1 mg/kg/day to 5000 mg/kg/day, more specifically 50 It may be mg/kg/day to 500 mg/kg/day, but is not limited thereto, and may vary depending on various factors such as age, health status, and complications of the subject to be administered.

The food composition according to the present specification is, for example, various foods such as chewing gum, caramel products, candies, ice creams, and confectionery, beverage products such as soft drinks, mineral water, alcoholic beverages, vitamins and minerals, etc. Health functional foods can

In addition to the above, the food composition of one aspect of the present specification includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the functional food compositions of the present specification may include natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is not so important, but is generally included in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition herein.

In one aspect of the present specification, the composition is not particularly limited in formulation, but a cosmetic composition having a formulation such as hair tonic, hair nourishing lotion, scalp treatment, hair treatment, hair rinse, hair shampoo, hair lotion, etc. can be

In addition, in each formulation, other components other than the above essential components can be suitably selected and formulated by those skilled in the art without difficulty depending on the type or purpose of use of other external preparations.

The cosmetic composition according to the present specification may be provided in any formulation suitable for topical application. For example, it may be provided in the form of a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder, a paste, a microneedle, a foam, or an aerosol composition. have. Compositions of such formulations can be prepared according to conventional methods in the art.

The cosmetic composition according to the present specification may further include functional additives and components included in general cosmetic compositions in addition to the extract of cornflower hydrangea. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract.

The cosmetic composition according to the present specification may include other ingredients that can give a synergistic effect to the main effect, preferably within a range that does not impair the main effect, in addition to the cornflower extract. In addition, the cosmetic composition according to the present specification may further include a moisturizer, an emollient, a surfactant, a UV absorber, a preservative, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cooling agent or a limiting agent. The blending amount of the component can be easily selected by those skilled in the art within the range that does not impair the purpose and effect of the present specification, and the blending amount may be 0.01 to 5 wt%, specifically 0.01 to 3 wt%, based on the total weight of the composition have.

Hereinafter, the present specification will be described in detail with reference to Examples, Comparative Examples and Test Examples of the present specification. These are merely presented by way of example in order to describe the present specification in more detail, and the scope of the present specification is not limited by these Examples, Comparative Examples and Test Examples.

[Preparation Example 1] Hydranase Serrata for acuminata (S. et z.) Preparation of Wilson extract

The leaves, fruits, and bark of Hydrangea Serrata for . acuminata (Siebold & Zucc) Wilson, chopped and dried in the shade, were mixed in a weight ratio of 7:2:1. 1 kg was extracted under reflux three times for 24 hours with 70% ethanol aqueous solution, cooled, and filtered with Whatman #5 filter paper. The filtered extract was concentrated under reduced pressure at 45°C or lower and freeze-dried at -60°C for 3 days.

The extract was prepared by dispersing/dissolving the concentrated under reduced pressure using ethanol in an amount of 5% by weight based on the total weight of the cornflower extract.

[Preparation Example 2] Preparation of Hydranase Serrata for Bujeri Nakai Extract

An extract of Hydrangea serrata for. buergeri Nakai. was prepared in the same manner as in Preparation Example 1.

[Preparation Example 3] Hydrant Serrata for Koreana Tea. Preparation of Li extract

Hydrant Serrata for Koreana Tea in the same manner as in Preparation Example 1. An extract of Hydrangea serrata for. coreana T. Lee was prepared.

[Preparation Example 4] Preparation of hydranase Serrata p. pertilis Nakai extract

An extract of Hydrangea serrata for. fertilis Nakai. was prepared in the same manner as in Preparation Example 1.

[Examples 1 to 4, Comparative Example 1]

Shampoos were prepared with the composition shown in Table 1 below (Hydrant Serrata for Acuminata (S. et z.) Wilson (Example 1), Hydra Serrata for Bujeri Nakai (Example 2), Hydrante Serrata for Koreana T. Lee (Example 3), Hydrant Serrata for Pertilis Nakai (Example 4)). Specifically, a surfactant and ethylene glycol distearate are added to purified water and heated to 80° C. to be uniformly dissolved, then slowly cooled to 40° C. under stirring, and the effectiveness of the cornflower extract according to the present specification in the mixture Powder, preservative, viscosity modifier, fragrance, and hair conditioning agent were added and mixed, followed by cooling to room temperature under stirring.

As a comparative example, Comparative Example 1 was made so as not to contain the corn hydrangea extract in Examples 1 to 4.

ingredient Examples 1 to 4 (wt%) Comparative Example 1 (wt%) Ammonium Lauryl Sulfate 10 8.0 Ammonium polyoxyethylene lauryl sulfate 5 5 Cocoamidopropyl Betaine 2 2 Ethylene glycol distearate 1.5 1.5 Cocoyl Monoethanolamide 0.8 0.8 Polyquaternium-10 0.2 0.2 Blue No. 1 0.0002 0.0002 Yellow No. 4 0.0001 0.0001 methylparaben 0.1 0.1 Spices 0.8 0.8 citric acid 0.1 0.1 dimethicone 1.0 1.0 Any one cornflower extract of Preparation Examples 1 to 4 5.0 - Purified water To 100 To 100

[Test Example 1] Dandruff reduction effect test

After selecting 84 males aged 19-35 with relatively high dandruff and using the shampoos of Examples 1 to 4 and Comparative Example 1 in 5 groups of 12 people for 1 month in the following manner, the reduction rate of dandruff was measured. did.

Wash hair with a normal shampoo before the start of the test, collect the dandruff accumulated for 2 days after washing, and wash the hair once every 2 days with the weight of the collected dandruff and the shampoos of Examples 1 to 4 and Comparative Example 1 2 The weight of daily accumulated dandruff was compared and evaluated. In this case, the accumulated dandruff was collected directly from the scalp using a vacuum suction device, the dandruff reduction rate was calculated based on Equation 1 below, and the results are shown in Table 2 below.

[Equation 1]

Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Subject 1 12 11 13 12 1.6 subject 2 18.9 18.7 19.2 18.7 -0.5 subject 3 10.5 10.1 11.1 10.7 -0.9 Subject 4 7.8 7.7 8.1 8.2 1.8 Subject 5 15 14.8 15.1 14.7 2.5 Subject 6 5.1 5.0 4.8 4.9 1.1 subject 7 9 9.1 8.8 8.8 5.9 subject 8 8.9 8.7 8.8 8.4 3.2 Subject 9 13.8 13.3 13.5 13.5 3.4 Subject 10 15.4 15.3 14.9 15.1 4.8 Subject 11 9.2 9.2 9.0 9.3 3.2 Subject 12 8.5 8.4 8.7 8.6 3.9 medium 11.175 10.94167 11.25 11.075 2.5 Standard Deviation 3.924776 3.862043 3.983945 3.741252 2.009522784

As can be seen from Table 2 above, in the case of Examples 1 to 4, compared to Comparative Example 1, the width of the decrease in the amount of dandruff in the subjects increased by about 2 times on average. Therefore, it can be seen that Examples 1 to 4 including the corn hydrangea extract exhibit a superior anti-dandruff effect than the case of not including the corn hydrangea extract.

[Test Example 2] Scalp itch prevention effect test

84 males and females aged 25 to 45 who feel relatively itchy scalp were selected and divided into 5 groups of 12 people each. After using the shampoos of Examples 1 to 4 and Comparative Example 1 once every 3 days for 2 weeks, the scalp The anti-itch effect was evaluated through the following evaluation criteria, and the results are shown in Table 3 below.

[Evaluation standard]

Very good - 5 points

Excellent - 4 points

Moderate - 3 points

Bad - 2 points

Very poor - 1 point

Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Evaluation results 4.1 4.2 4.2 4.0 2.1

As can be seen from Table 3 above, it can be seen that Examples 1 to 4 including the corn hydrangea extract exhibit a better effect of preventing scalp itching compared to Comparative Example 1 (without the corn hydrangea extract). .

[Test Example 3] Hair follicle dermal papilla cell proliferation effect test

Keratin protein constituting hair is produced in keratinocytes at the root of the hair, and these keratinocytes are differentiated from dermal papilla cells. In order to evaluate the activity of the dermal papilla cells of the composition according to the present specification, in the present specification, human primary dermal papilla cells isolated from human tissues were used. After isolation of primary dermal papilla cells from human tissue, 5% CO2, 37 in DMEM (Dulbecco's modified Eagle's medium; Gibco BRL, Gaithersburg, MD, USA) containing 10% fetal bovine serum (FBS). Incubated for 24 hours in an incubator maintained at °C.

The cultured human primary dermal dermal papilla cells were treated with the cornflower extract of Preparation Examples 1 to 4 as shown in Table 4, and cultured in a 96-well plate at 37° C. in an incubator for 24 hours. Comparative Example 2 is an untreated group that is not treated with the extract. After 24 hours of drug treatment, cell proliferation was measured using a WST-1 kit (Roche), and the results are shown in Table 5.

Ingredients (unit: ppm) Preparation Examples 1 to 4 Comparative Example 2 Cornflower extract of any one of Preparation Examples 1 to 4 5 -

Preparation Example 1 Preparation 2 Preparation 3 Preparation 4 Comparative Example 2 Cell proliferation rate (%) 123±1.8 122±1.5 121±1.8 123.4±1.7 100±1.8

From the results in Table 5, it can be seen that the dermal papilla cell proliferation effect of Examples 1 to 4 treated with the cornflower hydrangea extract is present. Therefore, it was confirmed that the composition containing the extract of cornflower hydrangea according to the present specification can exhibit the effect of promoting hair growth by promoting the proliferation of dermal papilla cells of hair follicles when treated on the scalp.

[Test Example 4] Hair loss prevention effect test

In order to confirm whether the present invention is effective in preventing hair loss of scalp hair, the effect of preventing hair loss was tested clinically. As a test method, only patients who satisfy all of the following criteria were enrolled as study subjects.

1) A person who is over 20 years of age and has good physical and mental health and is experiencing hair loss

2) Patients who will maintain the same hairstyle for the duration of the study

3) Patients who will not be stained during the study period

4) Patients who will not use special hair products or hair care and manipulation during the study period

Twenty voluntary subjects who met the above conditions were carefully selected and tested, and the specific status of each individual patient was identified and recorded on the evaluation sheet and tested.

The hair loss prevention effect was evaluated by measuring the number of hairs falling out when washing the hair. Before using the shampoo corresponding to Examples 1 to 4 and Comparative Example 1 and 3 months after using the shampoo were compared. The number of hairs falling out when washing the hair was recorded once a day, and the amount of hair loss was averaged in units of one month.

division Comparative Example 1 Example 1 Example 2 Example 3 Example 4 After 1 month of use 17.61 11.71 11.55 12.11 12.02 After 2 months of use 14.12 10.52 10.33 11.01 10.95 After 3 months of use 12.11 5.99 5.53 6.01 6.04

As such, it was found that the amount of hair loss significantly decreased by more than half as the number of months after using the shampoos corresponding to Examples 1 to 4 passed. From the above test results, it was confirmed that the present specification provides a composition that is safe to the living body and provides hair loss prevention and hair growth effects by using the extract of cornflower hydrangea.

Formulation examples of the composition according to an aspect of the present specification will be described below, but other various formulations are also applicable, which is not intended to limit the present specification, but only to be specifically described.

[Formulation Example 1] Soft capsule

Mixed with any one of Preparation Examples 1 to 4 cornflower extract 8mg, vitamin E 9mg, vitamin C 9mg, palm oil 2mg, hydrogenated vegetable oil 8mg, yellow wax 4mg and lecithin 9mg, and mixed according to a conventional method to obtain a soft capsule filling solution manufacture Soft capsules are prepared by filling 400 mg per capsule. And, separately from the above, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin, and 10 parts by weight of sorbitol solution, and the filling solution was filled to prepare a soft capsule containing 400 mg of the composition according to the present specification.

[Formulation Example 2] Tablet

After mixing 8 mg of cornflower extract of any one of Preparation Examples 1 to 4, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose, granulation using a fluidized bed dryer, a sugar ester (sugar ester) 6 mg is added. 500 mg of these compositions are compressed in a conventional manner to prepare tablets.

[Formulation Example 3] Drink agent

After mixing 8 mg of cornflower extract of any one of Preparation Examples 1 to 4, 9 mg of vitamin E, 9 mg of vitamin C, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide, 300 ml of purified water is added, and each bottle is filled to 200 ml each. do. After filling the bottle, it is sterilized at 130° C. for 4 to 5 seconds to prepare a drink.

[Formulation Example 4] Granules

8 mg of cornflower extract of any one of Preparation Examples 1 to 4, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous crystalline glucose, and 550 mg of starch were mixed, formed into granules using a fluidized bed granulator, and then filled into a bag to obtain granules. to manufacture

[Formulation Example 5] Injection

Injections were prepared in a conventional manner according to the composition shown in Table 7 below.

compounding ingredients content Cornflower extract of any one of Preparation Examples 1 to 4 10-50 mg Sterile distilled water for injection appropriate amount pH adjuster appropriate amount

[Formulation Example 6] Health functional food

Health functional foods were prepared in a conventional manner according to the composition shown in Table 8 below.

compounding ingredients content Cornflower extract of any one of Preparation Examples 1 to 4 20mg vitamin A acetate 70μg vitamin E 1.0mg vitamin B1 0.13mg vitamin B2 0.15mg vitamin B6 0.5mg vitamin B12 0.2μg vitamin C 10mg biotin 10 μg Nicotinamide 1.7mg folic acid 50 μg Calcium Pantothenate 0.5mg ferrous sulfate 1.75mg zinc oxide 0.82mg magnesium carbonate 25.3mg monobasic potassium phosphate 15mg Dibasic Calcium Phosphate 55mg Potassium Citrate 90mg calcium carbonate 100mg magnesium chloride 24.8mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food, but the composition is mixed as a preferred example, but the mixing ratio may be arbitrarily modified.

[Formulation Example 7] Health drink

Health drinks were prepared in a conventional manner according to the composition shown in Table 9 below.

compounding ingredients content Cornflower extract of any one of Preparation Examples 1 to 4 1000mg citric acid 1000mg oligosaccharide 100 g Taurine 1 g Purified water remaining amount

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and sterilized.

[Formulation Example 8] Softening lotion (skin lotion)

A softening lotion was prepared in a conventional manner according to the composition shown in Table 10 below.

compounding ingredients content (% by weight) Cornflower extract of any one of Preparation Examples 1 to 4 0.2 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 Carboxyvinyl Polymer 0.1 PEG-12 Nonylphenyl Ether 0.2 Polysorbate 80 0.4 ethanol 10.0 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 9] Nutrient lotion (milk lotion)

Nutrient lotion was prepared in a conventional manner according to the composition shown in Table 11 below.

compounding ingredients content (% by weight) Cornflower extract of any one of Preparation Examples 1 to 4 1.0 glycerin 3.0 butylene glycol 3.0 propylene glycol 3.0 Carboxyvinyl Polymer 0.1 beeswax 4.0 Polysorbate 60 1.5 Caprylic/Capric Triglycerides 5.0 squalane 5.0 Sorbitasequioleate 1.5 liquid paraffin 0.5 cetearyl alcohol 1.0 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 10] Nourishing Cream

Nutrient creams were prepared in a conventional manner according to the composition shown in Table 12 below.

compounding ingredients content (wt%) Cornflower extract of any one of Preparation Examples 1 to 4 2.0 glycerin 3.0 butylene glycol 3.0 liquid paraffin 7.0 beta glucan 7.0 Carbomer 0.1 Caprylic/Capric Triglycerides 3.0 squalane 5.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 Polysorbate 60 1.2 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 11] Massage Cream

A massage cream was prepared in a conventional manner according to the composition shown in Table 13 below.

compounding ingredients content (wt%) Cornflower extract of any one of Preparation Examples 1 to 4 2.0 glycerin 8.0 butylene glycol 4.0 liquid paraffin 45.0 beta glucan 7.0 Carbomer 0.1 Caprylic/Capric Triglycerides 3.0 beeswax 4.0 cetearyl glucoside 1.5 Sesqui oleate sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 12] Preparation of hair lotion

A hair lotion is prepared according to a conventional method with the composition shown in Table 14 below.

ingredient content (wt%) Purified water remaining amount The corn hydrangea extract of any one of 1 to 4 in preparation 2.0 glycerin 3.0 Butylene glycol 3.0 liquid paraffin 7.0 beta glucan 7.0 Carbomer 0.1 edelweiss extract 2.0 Caprylic Capric Triglyceride 3.0 squalene 5.0 Cetearyl Grucoside 1.5 Sorbitan Stearate 0.4 polysorbate 1.2 antiseptic appropriate amount incense appropriate amount pigment appropriate amount triethanolamine 0.1

As the specific parts of the present specification have been described in detail above, for those of ordinary skill in the art, these specific techniques are only preferred embodiments, and it is clear that the scope of the present specification is not limited thereto. Accordingly, the substantial scope of the present specification will be defined by the appended claims and their equivalents.

Claims (12)

A cosmetic composition for improving or preventing dandruff comprising an extract of Hydrangea serrata as an active ingredient. Hydrangea serrata ( Hydrangea serrata ) A food composition for improving or preventing dandruff comprising an extract as an active ingredient. 3. The method of claim 1 or 2,
The hydrangea, Hydrangea Serrata for . acuminata (Siebold & Zucc) Wilson, Hydrangea Serrata for Bujeri Nakai (Hydrangea serrata for buergeri Nakai.), Hydranje Serrata for Coreana Tea. Lee ( Hydrangea serrata for. coreana T. Lee) and Hydrangea serrata for. fertilis Nakai (Hydrangea serrata for. fertilis Nakai.) at least one composition selected from the group consisting of. 3. The method of claim 1 or 2,
The composition is at least one selected from the group consisting of whole cornflower hydrangea, woody leaves, stems, branches, bark, roots, seeds and fruits. 3. The method of claim 1 or 2,
The composition wherein the cornflower extract is at least one extract selected from the group consisting of water, an organic solvent, and a mixture thereof. 6. The method of claim 5,
The organic solvent is at least one composition selected from the group consisting of ethanol, butylene glycol and propylene glycol. 3. The method of claim 1 or 2,
The composition comprising the extract of cornflower hydrangea in an amount of 0.001 to 50% by weight based on the total weight of the composition. 8. The method of claim 7,
The composition comprising the cornflower extract in an amount of 0.1 to 1% by weight based on the total weight of the composition. According to claim 1,
The composition is a formulation selected from the group consisting of hair tonic, hair nourishing lotion, scalp treatment, hair treatment, hair rinse, hair shampoo and hair lotion. delete delete delete