KR102611916B1 - Composition for skin improvement comprising multiple extract - Google Patents
Composition for skin improvement comprising multiple extract Download PDFInfo
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- KR102611916B1 KR102611916B1 KR1020160113254A KR20160113254A KR102611916B1 KR 102611916 B1 KR102611916 B1 KR 102611916B1 KR 1020160113254 A KR1020160113254 A KR 1020160113254A KR 20160113254 A KR20160113254 A KR 20160113254A KR 102611916 B1 KR102611916 B1 KR 102611916B1
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- KR
- South Korea
- Prior art keywords
- skin
- lotus
- cymbidium
- nasturtium
- present
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Abstract
본 발명은 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 화장료 조성물에 관한 것으로서, 보다 상세하게는 피부 미백, 피부 탄력 증진, 주름 개선, 피부 보습, 항산화, 항염 등의 우수한 효과를 가지는 화장료 조성물에 관한 것이다. 본 발명의 조성물은 항염 및 항산화 효과가 우수하며, 멜라닌 총량 감소 효과를 나타내고 콜라겐 합성 촉진 효과, 및 보습 효과가 우수하여 피부 미백용, 탄력 증진용 또는 주름 개선용, 및 피부 보습용 화장료 조성물로서 사용할 수 있다. 또한, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물을 포함하는 조성물은 기능성 식품 조성물 및 의약외품 조성물로서 사용할 수 있다.The present invention relates to a cosmetic composition containing mixed extracts of lotus, cymbidium, and nasturtium as active ingredients, and more specifically, provides excellent effects such as skin whitening, skin elasticity enhancement, wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory properties. Branches relate to cosmetic compositions. The composition of the present invention has excellent anti-inflammatory and antioxidant effects, reduces the total amount of melanin, promotes collagen synthesis, and has an excellent moisturizing effect, so it can be used as a cosmetic composition for whitening skin, improving elasticity or improving wrinkles, and moisturizing the skin. You can. Additionally, the composition containing the mixed extract of lotus, cymbidium, and nasturtium of the present invention can be used as a functional food composition and quasi-drug composition.
Description
본 발명은 연꽃, 심비듐 및 금련화의 혼합 추출물을 포함하는 화장료 조성물에 관한 것으로서, 보다 구체적으로 상기 혼합 추출물을 유효성분으로 포함하는 피부 미백, 피부 탄력 증진, 주름 개선, 피부 보습, 항산화 또는 항염 등의 우수한 효과를 가지는 화장료 조성물에 관한 것이다. 또한, 본 발명은 연꽃, 심비듐 및 금련화의 혼합 추출물을 포함하는 기능성 식품 조성물 및 의약외품 조성물에 관한 것이다.The present invention relates to a cosmetic composition containing mixed extracts of lotus, cymbidium and nasturtium, and more specifically, to a cosmetic composition containing the mixed extract as an active ingredient for skin whitening, skin elasticity improvement, wrinkle improvement, skin moisturizing, antioxidant or anti-inflammatory. It relates to a cosmetic composition having excellent effects such as: Additionally, the present invention relates to a functional food composition and a quasi-drug composition containing mixed extracts of lotus, cymbidium, and nasturtium.
일반적으로 알려진 미백 성분으로서, 코지산(Kojic acid) 또는 알부틴 (Arbutin) 등과 같은 티로시나제 효소활성을 억제하는 물질, 하이드로퀴논 (Hydroquinone), 비타민 C(L-Ascorbic acid) 또는 이들의 유도체와 각종 식물 추출물이 있다. 이들은 멜라닌 색소의 합성을 저해함으로써, 피부 톤을 밝게 하여 피부 미백을 실현할 수 있을 뿐만 아니라, 자외선, 호르몬 또는 유전에 기인한 기미나 주근깨 등의 피부 과색소 침착증의 개선이 가능하다. 그러나 피부 적용 시, 자극과 발적 등의 안전성의 문제로 사용량의 제한이 있거나, 효과가 미미하여 실질적인 효과를 기대할 수 없는 문제점이 있다.Commonly known whitening ingredients include substances that inhibit tyrosinase enzyme activity such as kojic acid or arbutin, hydroquinone, vitamin C (L-Ascorbic acid) or their derivatives, and various plant extracts. There is. By inhibiting the synthesis of melanin pigment, they can not only brighten skin tone and achieve skin whitening, but also improve skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, or genetics. However, when applied to the skin, there is a limit to the amount of use due to safety issues such as irritation and redness, or the effect is so small that no practical effect can be expected.
콜라겐은 피부의 섬유아세포(Fibroblast)에서 생성되는 주요 기질 단백질로서 세포 외 간질에 존재하고, 중요한 기능으로는 피부의 기계적 견고성, 결합조직의 저항력과 조직의 결합력, 세포접착의 지탱, 세포 분할과 분화(유기체의 성장 혹은 상처 치유 시)의 유도 등이 알려져 있다. 이러한 콜라겐은 연령 및 자외선 조사에 의한 광 노화에 의해 감소하며, 콜라겐을 분해하는 콜라게나제 효소 활성으로 콜라겐 감소가 촉진된다. 이는 피부의 주름 형성과 밀접한 연관이 있다고 알려져 있다.Collagen is a major matrix protein produced by skin fibroblasts and is present in the extracellular matrix. Its important functions include mechanical rigidity of the skin, connective tissue resistance and tissue adhesion, support for cell adhesion, and cell division and differentiation. Induction (during the growth of organisms or wound healing) is known. Collagen decreases due to age and photoaging caused by ultraviolet irradiation, and collagen reduction is promoted by collagenase enzyme activity that decomposes collagen. This is known to be closely related to the formation of wrinkles on the skin.
아울러, 콜라겐은 진피층 내의 수분 보유에 중요한 역할을 하는 기질 단백질로 이러한 기질 단백질이 수분을 흡착하며, 이들이 이루는 구조 내부에 수분 보유력을 높여 피부가 적정 수분을 함유한 상태를 유지할 수 있도록 해주며, 이를 통해 피부의 탄력을 유지하는데 관여한다고 알려져 있다.In addition, collagen is a matrix protein that plays an important role in retaining moisture in the dermal layer. These matrix proteins absorb moisture and increase the water retention capacity within the structure they form, allowing the skin to maintain an appropriate level of moisture. It is known to be involved in maintaining skin elasticity.
현재, 피부 탄력 증진 및 주름 개선 화장료로는 레티노이드, 아데노신, 동물태반유래 단백질, 클로렐라 추출물 등이 알려져 있다. 가장 잘 알려져 있는 레티놀은 콜라겐 합성을 촉진하는 물질이지만 불안정하며, 피부 적용 시 자극, 발적 등의 안전성 문제로 사용량의 제한이 있으며, 클로렐라 추출물 등은 효과가 미미하여 실질적으로 피부 탄력 증진 및 주름 개선 효과를 기대하기가 어렵다고 알려져 있다.Currently, retinoids, adenosine, animal placenta-derived proteins, and chlorella extract are known as cosmetics for improving skin elasticity and improving wrinkles. Retinol, the most well-known substance, is a substance that promotes collagen synthesis, but it is unstable, and its usage is limited due to safety issues such as irritation and redness when applied to the skin. Chlorella extract, etc., has minimal effects and does not actually improve skin elasticity and improve wrinkles. It is known to be difficult to anticipate.
인체는 산화촉진물질과 산화억제물질이 균형을 이루고 있으나, 여러 가지 요인들로 인하여 이러한 균형 상태를 잃고 산화를 촉진하는 방향으로 기울게 되면, 생체 내에 산화적 스트레스(oxidative stress)가 유발되어 세포손상 및 병리적 질환을 유발하게 된다. 이러한 산화적 스트레스의 직접적 원인이 되는 활성 산소종 (reactive oxygen species, ROS)은 화학적으로 불안정하고 반응성이 높아 DNA, 단백질, 지질 및 탄수화물과 같은 여러 생체물질과 쉽게 반응할 수 있으며, 생체 내 고분자들을 공격하여 세포와 조직에 비가역적인 손상을 일으키거나 돌연변이, 세포독성 및 암 등을 초래하게 된다.The human body has a balance of pro-oxidation substances and antioxidant substances, but when this balance is lost due to various factors and the trend goes in the direction of promoting oxidation, oxidative stress is induced in the body, causing cell damage and damage. It causes pathological diseases. Reactive oxygen species (ROS), which are the direct cause of this oxidative stress, are chemically unstable and highly reactive, so they can easily react with various biomaterials such as DNA, proteins, lipids, and carbohydrates, and can destroy macromolecules in vivo. It attacks cells and tissues, causing irreversible damage or causing mutations, cytotoxicity, and cancer.
한편, 인체 내에서 대식세포는 병원체에 반응하여 종양 괴사 인자-α(tumor necrotic factor-α, TNF-α), 인터루킨-6(IL-6), 인터루킨-1β(IL-1β) 등과 같은 염증유발인자를 생성하고, 유도성 일산화질소 합성효소(inducible nitric oxide synthase, iNOS)와 사이클로옥시게나제-2(cyclooxygenase-2, COX-2)를 합성하여 일산화질소(NO) 및 프로스타글란딘(prostaglandin E2, PGE2)을 생성한다. 생리학적으로 NO는 세균과 종양을 제거하고 혈압을 조절하거나 신경 전달을 매개하는 등 다양한 역할을 한다. 그러나 염증 반응이 일어나게 되면 관련 세포들에서 iNOS의 발현이 증가하여 많은 양의 NO가 생성되고, 과도하게 생성된 NO는 조직의 손상, 유전자 변이, 신경 손상 등을 유발하며, 혈관 투과성을 증가시켜 부종 등의 염증 반응을 촉진시킨다.Meanwhile, within the human body, macrophages react to pathogens and induce inflammation such as tumor necrotic factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). Generates nitric oxide (NO) and prostaglandin (prostaglandin E2, PGE2) by synthesizing inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ) is created. Physiologically, NO plays a variety of roles, including eliminating bacteria and tumors, regulating blood pressure, and mediating nerve transmission. However, when an inflammatory response occurs, the expression of iNOS increases in related cells, producing a large amount of NO. Excessively produced NO causes tissue damage, genetic mutation, nerve damage, etc., and increases vascular permeability, leading to edema. Promotes inflammatory reactions such as:
염증 반응시에는 다양한 염증유발인자와 더불어 다양한 자유기(free radical)가 생성된다. 정상적인 자유기는 세포의 항상성 유지에 관여하며, 세포의 분화, 성장, 생존, 노화에 영향을 준다. 이러한 자유기 중 활성 산소종(reactive oxygen species, ROS)은 세포 내의 미토콘드리아에서 호흡과 면역 반응에 의한 산소의 산화와 환원 과정을 통해 끊임없이 생성된다. 유해한 ROS는 일반적으로 슈퍼옥사이드 디스뮤타제(superoxide dismutase), 카탈라제(catalase), 글루타치온 퍼옥시다제(glutathione peroxidase), 글루타민 리덕타제(glutamine reductase), 비타민 C, 비타민 E, 요산, 빌리루빈(bilirubin)과 같은 항산화 체계의 작용으로 제거된다. 그러나 자유기의 생성과 소거의 균형이 무너지게 되면 산화적 스트레스(oxidative stress)가 발생하게 되어 염증, 노화 또는 암과 같은 다양한 병리학적인 변화를 일으킨다. 실제로 많은 임상 질환에서 산화적 스트레스가 증가되는 것이 확인되었으며, 이러한 산화적 스트레스를 줄이기 위해 새로운 항산화 물질에 대한 다양한 연구가 이루어지고 있다.During an inflammatory reaction, various free radicals are generated along with various inflammation-inducing factors. Normal free radicals are involved in maintaining cellular homeostasis and affect cell differentiation, growth, survival, and aging. Among these free radicals, reactive oxygen species (ROS) are constantly generated in mitochondria within cells through the oxidation and reduction processes of oxygen caused by respiration and immune reactions. Harmful ROS generally include superoxide dismutase, catalase, glutathione peroxidase, glutamine reductase, vitamin C, vitamin E, uric acid, bilirubin and It is eliminated through the action of the same antioxidant system. However, when the balance between generation and elimination of free radicals is broken, oxidative stress occurs, causing various pathological changes such as inflammation, aging, or cancer. In fact, it has been confirmed that oxidative stress is increased in many clinical diseases, and various studies are being conducted on new antioxidant substances to reduce oxidative stress.
여러 가지 원인에 의한 환경으로부터의 자극, 스트레스, 화학적 시술 등으로부터 피부를 보호하고, 생태계 친화적이면서 환경과 인간에 무해한 성분에 관한 연구가 지속되고 있다.Research is continuing on ingredients that protect the skin from various causes such as environmental irritation, stress, and chemical treatments, and are eco-friendly and harmless to the environment and humans.
상기와 같이, 전술한 화장료 조성물들은 사람들에게 경제적 부담 및 고통을 주거나 부작용을 유발하고, 효과가 일시적이거나 또는 단편적인 약리학적 기작만을 사용하는 등의 문제점을 가지고 있어서, 효과가 보다 탁월하고 지속적이며, 부작용이 없는 조성물들의 요구가 증대되고 있는 실정이다. As described above, the above-described cosmetic compositions have problems such as causing economic burden and pain to people or causing side effects, and having temporary effects or using only fragmentary pharmacological mechanisms, so the effects are more excellent and lasting, The demand for compositions without side effects is increasing.
이러한 배경하에, 본 발명자들은 피부 미백, 피부 탄력 증진, 주름 개선, 피부 보습, 항산화 및 항염 효과가 우수한 물질에 관하여 연구를 수행하였으며, 이에 장미, 작약 및 아이리스의 혼합 추출물을 유효성분으로 포함하는 조성물이 멜라닌 총량 감소, 콜라겐 합성 촉진, 보습, 항염 및 항산화 효과를 나타냄을 규명하여, 이를 활용한 화장료 조성물, 기능성 식품 조성물 및 의약외품 조성물을 완성하기에 이르렀다.Against this background, the present inventors conducted research on substances with excellent skin whitening, skin elasticity enhancement, wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory effects, and developed a composition containing mixed extracts of rose, peony and iris as active ingredients. By discovering that it reduces the total amount of melanin, promotes collagen synthesis, moisturizes, has anti-inflammatory and antioxidant effects, it has led to the completion of cosmetic compositions, functional food compositions and quasi-drug compositions using it.
본 발명의 주된 목적은 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 화장료 조성물을 제공하는 것이다.The main object of the present invention is to provide a cosmetic composition containing mixed extracts of lotus, cymbidium, and nasturtium as active ingredients.
본 발명의 다른 목적은 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 기능성 식품 조성물을 제공하는 것이다.Another object of the present invention is to provide a functional food composition containing a mixed extract of lotus, cymbidium, and nasturtium as an active ingredient.
본 발명의 또 다른 목적은 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 의약외품 조성물을 제공하는 것이다.Another object of the present invention is to provide a quasi-drug composition containing a mixed extract of lotus, cymbidium, and nasturtium as an active ingredient.
상기 목적을 달성하기 위한, 본 발명의 하나의 양태는 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 화장료 조성물을 제공한다.In order to achieve the above object, one aspect of the present invention provides a cosmetic composition containing a mixed extract of lotus, cymbidium, and nasturtium as an active ingredient.
본 발명에서 사용되는 용어 "연꽃(Nelumbo nucifera Gaertn)"은 황실 귀족들은 남녀노소를 불문하고 배를 타고 호수에서 연꽃을 감상하는 '연꽃 관상 풍조' 가 존재 할 정도로 황실의 사랑을 받던 꽃이다. 연은 한의학에서도 매우 유용한 한약재로 사용하고 있는데, 열매에서부터 잎, 꽃, 연밥, 연의 암술, 뿌리에 이르기까지 연의 대부분을 활용하고 있다. 가장 많이 사용하는 부분은 열매로 기력을 돕고 오장을 보호해주며 갈증과 설사를 없애준다. 특히 마음을 안정시키는 효능이 있어 신경이 예민한 현대인들에게 유용한 약재다. 달면서도 쓴맛이 있는 잎은 혈액순환을 좋게 하고 어혈을 풀어주는 데 좋으며 타박상으로 인한 울혈을 치료하고, 상처에 찧어 붙이면 지혈효과가 크다. 또한 피부 미용과 아름다움의 비서라고 할 수 있는 '백별단방대전'에 피부를 관리하고 가꿀 수 있는 최고의 꽃으로 기재되어 있을 정도로, 예부터 피부 미용에 좋은 꽃으로 알려져 있다. The term "lotus flower (Nelumbo nucifera Gaertn)" used in the present invention is a flower that was loved by the imperial family to the extent that there was a 'lotus flower viewing trend' in which imperial nobles, regardless of age and gender, took a boat and admired the lotus flower in the lake. Lotus is also used as a very useful herbal medicine in Oriental medicine. Most of the lotus is used, from fruits to leaves, flowers, lotus rice, lotus pistils, and roots. The most used part is the fruit, which helps boost energy, protects the five intestines, and relieves thirst and diarrhea. In particular, it has the effect of stabilizing the mind, making it a useful herb for modern people with sensitive nerves. The sweet yet bitter leaves are good for improving blood circulation and relieving stagnant blood, treating congestion caused by bruises, and have a great hemostatic effect when pounded and applied to wounds. In addition, it has been known as a good flower for skin care since ancient times, as it is listed as the best flower for skin care and care in 'Baekbyeol Danbang Daejeon', which can be said to be the secretary of skin care and beauty.
본 발명에서 사용되는 용어 "심비듐(Cymbidium)"은 2000년간 군자의 상징으로 여겨지던 난꽃이다. 또한 강한 향기를 가지고 아름다워 예로부터 아껴지는 지생란의 하나이다. '귀족 꽃'이라 불릴 만큼 맑고 기품 있는 꽃의 형태와 향기를 가지고 있으며, 전통적으로 미용 효과를 인정받은 난 꽃을 이용해 우린 물을 피부를 부드럽고 윤택하게 만들기 위해 사용하였으며, 특히 결혼을 앞둔 귀족 여성들의 미용을 위한 비법으로 사용된 것으로 알려져 있다. The term "Cymbidium" used in the present invention is an orchid flower that has been considered a symbol of a gentleman for 2,000 years. It is also one of the terrestrial orchids that has been treasured since ancient times because of its strong fragrance and beauty. It has the form and scent of a flower so clear and elegant that it is called an 'aristocratic flower', and the water made with orchid flowers, which are traditionally recognized for their beauty effects, is used to make the skin soft and moist, especially for aristocratic women about to get married. It is known to be used as a secret for beauty.
본 발명에서 사용되는 용어 "금련화(Tropaeolum majus L.)"은 연잎을 닮았으나 땅에서 자라는 금빛의 연꽃이라 불리우며, '금련화' 중 꽃이 가장 아름다운 것은 Majus이다. 16세기 영국에서는 금련화가 보물 중 하나로 여겨졌으며, 스페인 정복자들이 페루의 잉카 인들로부터 금과 함께 유럽으로 가지고 왔던 귀하게 여겼던 보물 중 하나이다. 유럽 왕가의 건강을 위한 재료로 사용되던 귀한 원료로 알려져 있다. 성질이 차고 맛은 쓰고 독은 없는 것으로 알려져 있다. 열을 내리는 효능이 있으며, 급성 결막염에도 효능이 있는 것으로 알려져 있다. The term "Tropaeolum majus L." used in the present invention refers to a golden lotus flower that resembles a lotus leaf but grows on the ground. Among the 'Tropaeolum majus L.' flowers, the most beautiful flower is Majus. In the 16th century in England, nasturtiums were considered one of the treasures, and were one of the treasures that the Spanish conquistadors brought to Europe along with gold from the Incas of Peru. It is known as a valuable raw material used for the health of European royal families. It is known to be cold in nature, bitter in taste, and non-poisonous. It is effective in reducing fever and is known to be effective in acute conjunctivitis.
본 발명에서의, "추출물(extract)"은, 목적하는 물질을 다양한 용매에 침지한 다음, 상온, 저온 또는 가온 상태에서 일정시간 동안 추출하여 수득한 액상성분, 상기 액상성분으로부터 용매를 제거하여 수득한 고형분 등의 결과물을 의미한다. 뿐만 아니라, 상기 결과물에 더하여, 상기 결과물의 희석액, 이들의 농축액, 이들의 조정제물, 정제물 등을 모두 포함하는 것으로 포괄적으로 해석될 수 있다.In the present invention, “extract” refers to a liquid component obtained by immersing the desired substance in various solvents and then extracting the substance for a certain period of time at room temperature, low temperature, or heated state, and removing the solvent from the liquid component. It refers to the result of solid content, etc. In addition, in addition to the above results, it can be comprehensively interpreted to include all dilutions of the results, concentrates thereof, crude preparations, purifications, etc. of the above results.
본 발명에 있어서, 상기 추출물은 연꽃, 심비듐, 금련화 추출물을 의미한다. 상기 연꽃, 심비듐, 금련화 추출물은 천연, 잡종, 변종식물의 다양한 기관으로부터 추출될 수 있고, 예를 들어 뿌리, 지상부, 줄기, 잎, 꽃, 열매의 몸통, 열매의 껍질뿐만 아니라 식물 조직으로부터 추출 가능하다. 상기 연꽃, 심비듐, 금련화 추출물은 물 또는 다양한 유기용매 등으로 추출하여 수득할 수 있다. 이때, 사용되는 유기용매는 추출물을 수득할 수 있는 한, 특별히 이에 제한되지 않으나, 구체적으로는 물, 극성용매 또는 비극성용매가 될 수 있고, 보다 구체적으로는 물, 탄소수 1 내지 6의 저급 알코올(메탄올, 에탄올, 프로판올 또는 부탄올 등), 이들의 혼합용매 일 수 있으며, 가장 구체적으로는 메탄올 또는 이의 혼합용매일 수 있다. 또한, 본 발명에서 추출물의 용매로 당류를 포함할 수 있다. 구체적으로 당류는 꿀, 로얄젤리, 설탕, 과당, 당알콜류, 프락토올리고탕 등이 사용될 수 있으나 이에 제한되지 않는다.In the present invention, the extract refers to lotus, cymbidium, and nasturtium extracts. The lotus, cymbidium, and nasturtium extracts can be extracted from various organs of natural, hybrid, and variant plants, for example, roots, aerial parts, stems, leaves, flowers, fruit bodies, fruit peels, as well as plant tissues. Extractable. The lotus, cymbidium, and nasturtium extracts can be obtained by extraction with water or various organic solvents. At this time, the organic solvent used is not particularly limited as long as it can obtain an extract, but may specifically be water, a polar solvent, or a non-polar solvent, and more specifically, water, a lower alcohol having 1 to 6 carbon atoms ( methanol, ethanol, propanol, or butanol, etc.), or a mixed solvent thereof, and most specifically, it may be methanol or a mixed solvent thereof. Additionally, in the present invention, sugars may be included as a solvent for the extract. Specifically, sugars such as honey, royal jelly, sugar, fructose, sugar alcohols, and fructooligosaccharide may be used, but are not limited thereto.
또한, 상기 추출물을 수득하기 위한 방법 연꽃, 심비듐, 금련화 추출물을 수득할 수 있는 한, 특별히 이에 제한되지 않으나, 구체적으로는 상기 연꽃, 심비듐, 금련화 의 뿌리, 줄기, 잎, 열매, 꽃, 이들의 건조물, 가공물 등을 상기 용매에 침지하고, 상온에서 추출하는 냉침추출법, 가열추출법, 초음파를 가하여 추출하는 초음파추출법, 환류냉각기를 이용한 환류추출법 등의 방법을 사용할 수 있다.In addition, the method for obtaining the extract is not particularly limited as long as the lotus, cymbidium, and nasturtium extracts can be obtained, but specifically, the roots, stems, leaves, and fruits of the lotus, cymbidium, and nasturtium extracts, Flowers, their dried products, processed products, etc. can be immersed in the solvent and extracted at room temperature using methods such as cold extraction, heat extraction, ultrasonic extraction by applying ultrasonic waves, and reflux extraction using a reflux condenser.
본 발명에서 사용되는 용어 "혼합 추출물"은, 연꽃, 심비듐, 금련화의 각 성분의 추출물을 적절한 중량 배합비로 혼합한 추출물일 수 있고, 또한, 연꽃, 심비듐, 금련화를 적절한 중량 배합비로 혼합한 후 추출한 것 일 수 있다. 혼합 추출물은 각각의 연꽃, 심비듐, 금련화 단일 추출물보다 피부 미백, 피부 탄력 증진 또는 주름 개선, 피부 보습, 피부 노화 방지, 항산화, 및 피부 염증 개선에 효과적임을 확인하였는바, 우수한 시너지 작용을 하는 것을 알 수 있다. The term "mixed extract" used in the present invention may be an extract obtained by mixing the extracts of each component of lotus, cymbidium, and nasturtium in an appropriate weight mixing ratio. Additionally, the lotus, cymbidium, and nasturtium may be mixed in an appropriate weight mixing ratio. It may be extracted after mixing. The mixed extract was confirmed to be more effective in skin whitening, improving skin elasticity or wrinkles, moisturizing the skin, preventing skin aging, antioxidant, and improving skin inflammation than the individual lotus, cymbidium, and nasturtium extracts alone. It has an excellent synergistic effect. You can see that
연꽃 추출물, 심비듐 추출물 및 금련화 추출물의 중량 배합비는 1~25 : 1~25 : 1~25의 중량비(w/w), 구체적으로는 1~15 : 1~15 : 1~15의 중량비(w/w), 보다 구체적으로는 1~5 : 1~5 : 1~5의 중량비(w/w)일 수 있고, 동량의 중량 배합비를 가질 수 있다. The weight mixing ratio of lotus extract, cymbidium extract, and nasturtium extract is a weight ratio (w/w) of 1 to 25: 1 to 25: 1 to 25, specifically a weight ratio of 1 to 15: 1 to 15: 1 to 15 (w/w). w/w), more specifically, it may be a weight ratio (w/w) of 1 to 5: 1 to 5: 1 to 5, and may have a weight mixing ratio of the same amount.
본 발명에서는 상기 혼합 추출물에 추가로 당류를 포함할 수 있다. 당류는 꿀, 로얄젤리, 설탕, 과당, 당알콜류, 프락토올리고탕 등이 사용되나 이에 제한되지 않고 당해 기술 분야에서 통상적으로 사용되는 물질이라면 어느 것이든 사용할 수 있다. 보다 구체적으로는 당류로서 꿀, 로얄젤리를 사용할 수 있다. In the present invention, sugars may be additionally included in the mixed extract. Sugars include, but are not limited to, honey, royal jelly, sugar, fructose, sugar alcohols, and fructooligosaccharide, and any substance commonly used in the art can be used. More specifically, honey and royal jelly can be used as sugars.
본 발명에서 사용되는 용어 "꿀"은 벌이 꽃의 꿀샘에서 채집하여 먹이로 저장해 둔 것을 의미한다. 꿀은 벌의 종류에 따라 토종꿀과 양봉꿀로 나누어지고, 꽃에 따라 아카시아꿀, 싸리꿀, 유채꿀, 밤꿀, 메밀꿀 등으로 불리며, 꽃의 종류에 따라 빛깔과 맛이 달라진다. 꿀은 약 80% 가량이 탄수화물로 과당이 36~38%, 포도당이 34~36%, 설탕과 덱스트린이 2~3%이다. 그 밖에는 단백질 0.2~0.3%, 회분 0.05~0.2%, 비타민 B 복합체인 B1, B2, B6, 판토텐산 등과 개미산, 젖산, 사과산, 색소, 고무질, 왁스, 효소 등이 함유되어 있다. 우리나라에서 꿀은 식용과 약용으로 널리 이용되며, 감미료로 사용되기도 한다.The term “honey” used in the present invention refers to what bees collect from the nectar glands of flowers and store it as food. Honey is divided into native honey and beekeeping honey depending on the type of bee, and is called acacia honey, bush honey, rape honey, chestnut honey, buckwheat honey, etc. depending on the flower. The color and taste vary depending on the type of flower. Honey is about 80% carbohydrates, with 36-38% fructose, 34-36% glucose, and 2-3% sugar and dextrin. In addition, it contains 0.2-0.3% protein, 0.05-0.2% ash, vitamin B complex B1, B2, B6, pantothenic acid, formic acid, lactic acid, malic acid, pigment, gum, wax, enzyme, etc. In Korea, honey is widely used for edible and medicinal purposes, and is also used as a sweetener.
본 발명에서 사용되는 용어 "로얄젤리"는 일벌들이 '왕에게 바치는 선물'로, 여왕벌의 먹이를 의미한다. 로얄젤리는 단백질, 지방, 탄수화물 및 각종 미량원소를 고루 함유한 신비의 영양 물질과 아직도 밝혀지지 않은 R이라는 성분을 함유하여 다양한 효능을 가지는 것으로 알려져 있다. 또한 로얄젤리의 성분자체가 영양만점에 피부 미용에도 효과적인 것으로 알려져 있다.The term "royal jelly" used in the present invention refers to a 'gift offered to the king' by worker bees and food for the queen bee. Royal jelly is known to have various effects as it contains a mysterious nutritional substance containing proteins, fats, carbohydrates, and various trace elements, as well as an ingredient called R, which has not yet been discovered. In addition, royal jelly itself is known to be nutritious and effective for skin care.
본 발명의 일 실시예에 따르면, 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀, 로얄젤리 등의 당류가 추가된 경우에 연꽃, 심비듐, 금련화 각각의 단일 추출물과 비교하여, 콜라겐합성이 증진되고, 멜라닌 생성이 억제되며, 보습인자인 HAS(Hyaluronan Synthases) 발현이 증가되고, 라디칼 소거능과, NO 생성이 억제되는 것을 확인하였다. According to one embodiment of the present invention, when sugars such as honey and royal jelly are added to the mixed extract of lotus, cymbidium, and nasturtium, collagen synthesis increases compared to single extracts of lotus, cymbidium, and nasturtium. It was confirmed that melanin production was enhanced, melanin production was suppressed, expression of HAS (Hyaluronan Synthases), a moisturizing factor, was increased, and radical scavenging ability and NO production were suppressed.
이를 통해, 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 및/또는 로얄젤리와 같은 당류가 추가되는 경우 추출물과 시너지 작용을 일으켜, 피부 미백, 피부 탄력, 피부 보습, 항염 및 항산화에 더 우수한 효과를 가짐을 알 수 있었다. Through this, when sugars such as honey and/or royal jelly are added to the mixed extract of lotus, cymbidium, and nasturtium, it creates a synergistic effect with the extract, resulting in better skin whitening, skin elasticity, skin moisturizing, anti-inflammatory, and antioxidant effects. It was found that it had.
본 발명에서 상기 화장료 조성물은 피부 미백, 피부 탄력증진 및 주름 개선, 피부 보습, 항산화, 항염증 등의 효과를 가질 수 있으나, 이에 제한되지 않는다. In the present invention, the cosmetic composition may have effects such as skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory effects, but is not limited thereto.
본 발명에서의 "피부 미백"이라 함은 멜라닌 색소의 합성을 저해함으로써 피부 톤을 밝게 할 뿐만 아니라, 자외선, 호르몬 또는 유전에 기인한 기미나 주근깨 등의 피부의 과색소 침착을 개선하는 것을 말한다. “Skin whitening” in the present invention refers to not only brightening skin tone by inhibiting the synthesis of melanin pigment, but also improving hyperpigmentation of the skin such as spots or freckles caused by ultraviolet rays, hormones, or genetics.
구체적인 일 실시예에 따르면, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 및/또는 로얄젤리를 더한 조성물을 멜라노마 세포에 처리한 후 멜라닌의 총량을 측정한 결과, 혼합 추출물이 대조군 또는 단일추출물들과 비교할 때 멜라노마 세포에 대하여 더 우수한 멜라닌 생성 억제 효과가 있음을 나타내므로 피부 미백 용도로 사용할 수 있음을 확인하였다(표 3). According to one specific embodiment, melanoma cells are treated with a mixed extract of lotus, cymbidium, and nasturtium of the present invention, or a composition obtained by adding honey and/or royal jelly to a mixed extract of lotus, cymbidium, and nasturtium, and then melanin As a result of measuring the total amount, it was confirmed that the mixed extract had a better melanin production inhibition effect on melanoma cells compared to the control or single extracts, and thus could be used for skin whitening (Table 3).
이를 통해, 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 및/또는 로얄젤리가 추가되는 경우에 단일 추출물에 비하여 시너지 작용을 일으켜, 피부 미백에 더 우수한 효과를 가짐을 알 수 있었다. Through this, when honey and/or royal jelly are added to a mixed extract of lotus, cymbidium, and nasturtium or a mixed extract of lotus, cymbidium, and nasturtium, it produces a synergistic effect compared to a single extract, making it superior for skin whitening. It was found to be effective.
본 발명에서의 "피부 탄력 증진"이란 피부가 쳐지거나 늘어지는 정도를 완화시켜주는 것으로, 엘라스틴으로 구성된 탄력섬유가 콜라겐(collagen)이라고 하는 교원섬유와 함께 존재하는데, 엘라스틴과 콜라겐이 충분히 존재하는 상태에서 피부 탄력이 유지되는 것을 말한다. 본 발명에 있어서, "주름 개선"이란 피부에 주름이 생성되는 것을 억제 또는 저해하거나, 이미 생성된 주름을 완화시키는 것을 말한다. In the present invention, "promoting skin elasticity" refers to alleviating the degree of sagging or sagging skin, in which elastic fibers made of elastin exist together with collagen fibers called collagen, a state in which sufficient elastin and collagen are present. This means that skin elasticity is maintained. In the present invention, “wrinkle improvement” refers to suppressing or inhibiting the formation of wrinkles on the skin or alleviating wrinkles that have already formed.
구체적인 일 실시예에 따르면, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 또는 로얄젤리를 더한 조성물을 처리한 HF(Human Fibroblast) 세포를 배양하여 프로 콜라겐의 합성을 측정한 결과, 콜라겐 합성 효과가 혼합 추출물에서 가장 우수함을 확인하였다(표 2). According to one specific embodiment, HF (Human Fibroblast) cells treated with a mixed extract of lotus, cymbidium, and nasturtium of the present invention or a composition obtained by adding honey or royal jelly to a mixed extract of lotus, cymbidium, and nasturtium are cultured. As a result of measuring the synthesis of pro-collagen, it was confirmed that the collagen synthesis effect was the best in the mixed extract (Table 2).
이를 통해, 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 및/또는 로얄젤리가 추가되는 경우에 단일 추출물에 비하여 시너지 작용을 일으켜, 피부 탄력 증진에 더 우수한 효과를 가짐을 알 수 있었다. Through this, when honey and/or royal jelly are added to a mixed extract of lotus, cymbidium, and nasturtium, or a mixed extract of lotus, cymbidium, and nasturtium, it produces a synergistic effect compared to a single extract, further improving skin elasticity. It was found to have excellent effects.
본 발명에서의 "피부 보습"이란 피부에 수분감을 증가시켜주고, 촉촉한 상태를 유지시키는 것을 의미한다. 피부 보습 효과는 피부의 주름개선, 탄력도 증가에 도움을 줄 수 있다.“Skin moisturizing” in the present invention means increasing moisture in the skin and maintaining a moist state. The skin moisturizing effect can help improve skin wrinkles and increase elasticity.
구체적인 일 실시예에 따르면, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 및/또는 로얄젤리를 더한 조성물에 각질형성세포(HaCaT, Human keratinocyte)을 배양하고, HAS3 mRNA양을 비교하여 보습력을 실험한 결과, 상기 혼합 추출물을 처리한 경우가 대조군과 단일추출물에 비하여 HAS3 mRNA 발현이 증가됨을 확인하였다(표 4). According to one specific embodiment, the mixed extract of lotus, cymbidium, and nasturtium of the present invention, or the mixed extract of lotus, cymbidium, and nasturtium plus honey and/or royal jelly, contains keratinocytes (HaCaT, Human keratinocyte). ) was cultured and the moisturizing power was tested by comparing the amount of HAS3 mRNA. As a result, it was confirmed that HAS3 mRNA expression was increased when treated with the mixed extract compared to the control and single extract (Table 4).
이러한 결과를 통해 상기 혼합 추출물이 피부 보습 효과를 나타낼 수 있음을 알 수 있었다.These results showed that the mixed extract could have a skin moisturizing effect.
이를 통해, 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 및/또는 로얄젤리가 추가되는 경우에 단일 추출물에 비하여 시너지 작용을 일으켜, 피부 보습에 더 우수한 효과를 가짐을 알 수 있었다.Through this, when honey and/or royal jelly are added to a mixed extract of lotus, cymbidium, and nasturtium, or a mixed extract of lotus, cymbidium, and nasturtium, it produces a synergistic effect compared to a single extract, providing better skin moisturization. It was found to be effective.
본 발명에서의 "항산화"란 산화를 억제하는 작용을 의미하는 것으로, 인체는 산화촉진물질(prooxidant)과 산화억제물질(antioxidant)이 균형을 이루고 있으나 여러 가지 요인들에 의하여 이런 균형상태가 불균형을 이루게 되고 산화촉진 쪽으로 기울게 되면, 산화적 스트레스(oxidative stress)가 유발되어 잠재적인 세포손상 및 병리적 질환을 일으키게 된다. 이러한 산화적 스트레스의 직접적 원인이 되는 활성 산소종(reactive oxygen species, ROS)은 불안정하고 반응성이 높아 여러 생체물질과 쉽게 반응하고, 체내 고분자들을 공격하여 세포와 조직에 비가역적인 손상을 일으키거나 돌연변이, 세포독성 및 발암 등을 초래하게 된다. NO, HNO2, ONOO-와 같은 활성 질소종(reactive nitrogen species, RNS)은 염증 반응 시 대식세포 호중구 및 다른 면역 세포 들의 면역반응으로 인해 다량 생성되며, 이때 ROS도 같이 생성된다. 상기와 같은 활성산소는 체내에서 세포를 산화시켜 파괴시키며, 그에 따라 각종 질환에 노출되게 된다. 따라서, 본 발명의 혼합 추출물을 화장품에 포함시키면 항산화 효과를 달성함으로써, 건강증진에 기여할 수 있다.In the present invention, "antioxidation" refers to the action of suppressing oxidation. The human body has a balance of prooxidants and antioxidants, but this balance can become unbalanced due to various factors. When this is achieved and the tendency is toward promoting oxidation, oxidative stress is induced, causing potential cell damage and pathological diseases. Reactive oxygen species (ROS), which are the direct cause of this oxidative stress, are unstable and highly reactive, easily reacting with various biomaterials, and attacking macromolecules in the body, causing irreversible damage to cells and tissues, mutations, and mutations. It causes cytotoxicity and carcinogenesis. Reactive nitrogen species (RNS), such as NO, HNO 2 , and ONOO - , are produced in large quantities due to the immune response of macrophages, neutrophils, and other immune cells during inflammatory reactions, and ROS is also produced at this time. The above-described free radicals oxidize and destroy cells in the body, thereby exposing them to various diseases. Therefore, if the mixed extract of the present invention is included in cosmetics, it can contribute to health promotion by achieving an antioxidant effect.
구체적인 일 실시예에 따르면, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물의 DPPH 라디칼 소거능(표 5)을 분석하여 강한 항산화능을 갖는 것을 확인하였다. 상기 혼합 추출물이 단일추출물에 비하여 IC50값이 감소됨을 확인하였다. 이러한 결과를 통하여 상기 혼합 추출물이 항산화 효과를 나타낼 수 있음을 알 수 있었다. According to one specific example, the DPPH radical scavenging ability (Table 5) of the mixed extract of lotus, cymbidium, and nasturtium of the present invention was analyzed and it was confirmed to have strong antioxidant ability. It was confirmed that the IC 50 value of the mixed extract was reduced compared to the single extract. These results showed that the mixed extract can exhibit antioxidant effects.
이를 통해, 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 및/또는 로얄젤리가 추가되는 경우에 단일 추출물에 비하여 시너지 작용을 일으켜, 항산화에 더 우수한 효과를 가짐을 알 수 있었다.Through this, when honey and/or royal jelly are added to a mixed extract of lotus, cymbidium, and nasturtium or a mixed extract of lotus, cymbidium, and nasturtium, a synergistic effect occurs compared to a single extract, resulting in a superior antioxidant effect. It was found that it had.
본 발명에서의 "항염증"은 염증을 억제하는 작용을 의미하는 것으로, 염증반응의 조절은 대단히 복잡한 것으로 알려져 있는데, 이는 생체 내 복구체계의 증강 및 손상을 감소시키기 위한 것으로 알려져 있다. 그러나 반복되는 조직의 손상이나 재생에 의해 염증반응이 지속되면, 염증관련 세포에서 ROS와 RNS가 과다 생성되고 그 결과로 영구적인 유전자의 변형이 야기된다. 이처럼 ROS와 RNS는 생체 내 여러 가지 세포의 작용을 조절하는 염증 반응과 깊이 관련되어 있다. 염증 과정 중에는 많은 양의 염증유도 사이토카인(proinflammatory cytokines), 아질산(nitric oxide, NO) 그리고 프로스타글란딘(prostaglandin E2, PGE2)이 유도성 일산화질소 합성효소(inducible nitric oxide synthase, iNOS)와 사이클로옥시게나아제(cyclooxygenase-2, COX-2)에 의해 생성된다. 염증은 다양한 염증성 질환을 유발하는 원인으로써, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물을 포함하는 조성물은 항염증 작용을 통해 다양한 염증성 질환에 대한 예방 및 개선 효과를 가질 수 있다.“Anti-inflammatory” in the present invention refers to the action of suppressing inflammation, and the control of inflammatory response is known to be very complex, and is known to enhance the in vivo repair system and reduce damage. However, if the inflammatory response continues due to repeated tissue damage or regeneration, ROS and RNS are excessively produced in inflammation-related cells, resulting in permanent genetic modification. As such, ROS and RNS are deeply related to the inflammatory response that regulates the actions of various cells in vivo. During the inflammatory process, large amounts of proinflammatory cytokines, nitric oxide (NO), and prostaglandin E2 (PGE2) are released into inducible nitric oxide synthase (iNOS) and cyclooxygenase. It is produced by (cyclooxygenase-2, COX-2). Inflammation is a cause of various inflammatory diseases, and the composition containing the mixed extract of lotus, cymbidium, and nasturtium of the present invention can have a preventive and ameliorating effect on various inflammatory diseases through anti-inflammatory action.
구체적인 일 실시예에 따르면, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물의 NO 생성 억제 효과를 분석한 결과, 단일 추출물에 비하여 혼합 추출물에서 동일 농도로 처리시 더욱 높은 NO 생성 억제능을 확인하였는 바, 상기 혼합 추출물이 강한 항염증 효과를 갖는 것을 확인하였다(표 6). According to one specific example, as a result of analyzing the NO production inhibition effect of the mixed extract of lotus, cymbidium, and nasturtium of the present invention, it was confirmed that the mixed extract had a higher NO production inhibition ability when treated at the same concentration compared to the single extract. As a result, it was confirmed that the mixed extract had a strong anti-inflammatory effect (Table 6).
이를 통해, 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 꿀 및/또는 로얄젤리가 추가되는 경우에 단일 추출물에 비하여 시너지 작용을 일으켜, 항염증에 더 우수한 효과를 가짐을 알 수 있었다.Through this, when honey and/or royal jelly are added to a mixed extract of lotus, cymbidium, and nasturtium or a mixed extract of lotus, cymbidium, and nasturtium, it produces a synergistic effect compared to a single extract, making it superior to anti-inflammatory agents. It was found to be effective.
본 발명에 따른 화장료 조성물은 용액, 외용연고, 크림, 폼, 영양화장수, 유연화장수, 팩, 유연수, 유액, 메이크업베이스, 에센스, 비누, 액체 세정료, 입욕제, 선 스크린크림, 선오일, 현탁액, 유탁액, 페이스트, 겔, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 패취 및 스프레이로 구성된 군으로부터 선택되는 제형으로 제조할 수 있으나, 이에 제한되는 것은 아니다.The cosmetic composition according to the present invention includes solution, external ointment, cream, foam, nourishing lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleanser, bath agent, sunscreen cream, sun oil, suspension, Can be prepared in a formulation selected from the group consisting of emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. That is not the case.
또한, 본 발명의 화장료 조성물은 일반 피부 화장료에 배합되는 화장품학적으로 허용 가능한 담체를 1 종 이상 추가로 포함할 수 있으며, 통상의 성분으로 예를 들면 유분, 물, 계면활성제, 보습제, 저급 알콜, 증점제, 킬레이트제, 색소, 방부제, 향료 등을 적절히 배합할 수 있으나, 이에 제한되는 것은 아니다.In addition, the cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers that are blended with general skin cosmetics, and common ingredients include, for example, oil, water, surfactant, moisturizer, lower alcohol, Thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately mixed, but are not limited thereto.
본 발명의 화장료 조성물에 포함되는 화장품학적으로 허용 가능한 담체는 제형에 따라 다양하다.Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation.
본 발명의 제형이 연고, 페이스트, 크림 또는 젤인 경우에는, 담체 성분으로서 동물성 유, 식물성 유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크, 산화아연 또는 이들의 혼합물이 이용될 수 있다.When the formulation of the present invention is an ointment, paste, cream or gel, the carrier ingredient may be animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these can be used.
본 발명의 제형이 파우더 또는 스프레이인 경우에는, 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록사이드, 칼슘 실케이트, 폴리아미드 파우더 또는 이들의 혼합물이 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진제를 포함할 수 있다.When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, or mixtures thereof may be used as the carrier ingredient, and especially in the case of a spray, chloro May contain propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는, 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되며, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알콜, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일이 이용될 수 있으며, 특히, 목화씨 오일, 땅콩 오일, 옥수수 배종 오일, 올리브오일, 피마자 오일 및 참깨 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 이용될 수 있다.When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan. there is.
본 발명의 제형이 현탁액인 경우에는, 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알콜, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and miso. Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.
본 발명의 제형이 비누인 경우에는 담체 성분으로서 지방산의 알칼리 금속 염, 지방산 헤미에스테르 염, 지방산 단백질 히드롤리제이트, 이세티오네이트, 라놀린 유도체, 지방족 알콜, 식물성 유지, 글리세롤, 당 등이 이용될 수 있다.When the formulation of the present invention is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier ingredients. You can.
본 발명의 화장료 조성물은 상기 연꽃, 심비듐 및 금련화의 혼합 추출물 또는 연꽃, 심비듐 및 금련화의 혼합 추출물에 추가로 꿀 또는 로얄젤리의 당류를 포함할 뿐만 아니라, 화장료 조성물에 통상적으로 이용되는 보조제 예컨대 친수성 또는 친지성 겔화제, 친수성 또는 친지성 활성제, 보존제, 항산화제, 용매, 방향제, 충전제, 차단제, 안료, 흡취제 및 염료 등을 함유할 수 있다.The cosmetic composition of the present invention not only contains sugars of honey or royal jelly in addition to the mixed extract of lotus, cymbidium, and nasturtium or the mixed extract of lotus, cymbidium, and nasturtium, but also contains sugars commonly used in cosmetic compositions. It may contain auxiliaries such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic activators, preservatives, antioxidants, solvents, fragrances, fillers, blocking agents, pigments, odorants and dyes.
상기의 목적을 달성하기 위한 또 다른 양태로서, 본 발명은 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 기능성식품 조성물을 제공한다.As another aspect for achieving the above object, the present invention provides a functional food composition containing mixed extracts of lotus, cymbidium, and nasturtium as an active ingredient.
본 발명에서 상기 기능성 식품 조성물은 추가로 당류를 포함할 수 있다. 당류는 꿀, 로얄젤리, 설탕, 과당, 당알콜류, 프락토올리고탕 등이 사용되나 이에 제한되지 않고 당해 기술 분야에서 통상적으로 사용되는 물질이라면 어느 것이든 사용할 수 있다.In the present invention, the functional food composition may additionally include sugars. Sugars include, but are not limited to, honey, royal jelly, sugar, fructose, sugar alcohols, and fructooligosaccharide, and any substance commonly used in the art can be used.
본 발명에서 상기 기능성식품 조성물은 피부 미백, 피부 탄력증진 및 주름 개선, 피부 보습, 항산화, 항염증 등의 효과를 가질 수 있으나, 이에 제한되지 않는다. In the present invention, the functional food composition may have effects such as skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory effects, but is not limited thereto.
상기 연꽃, 심비듐, 금련화, 추출물, 혼합 추출물, 꿀, 로얄젤리, 피부 미백, 피부 탄력증진 및 주름 개선, 피부 보습, 항산화, 항염증은 상기에서 설명한 바와 같다. 상기 기능성 식품 조성물은 건강기능식품의 형태로 사용될 수 있으나, 이에 제한되는 것은 아니다.The lotus flower, cymbidium, nasturtium, extract, mixed extract, honey, royal jelly, skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory are as described above. The functional food composition may be used in the form of a health functional food, but is not limited thereto.
본 발명의 기능성 식품 조성물에 포함된 연꽃, 및 금련화의 혼합 추출물을 유효성분으로 포함하는 기능성 식품 조성물은 이의 추출물, 이의 분획물 또는 이의 가공물의 형태로 포함될 수 있다. 또한 상기 조성물은 유효성분 이외에 식품학적으로 허용 가능한 식품보조첨가제를 포함할 수 있다.The functional food composition containing the mixed extract of lotus flower and nasturtium flower included in the functional food composition of the present invention as an active ingredient may be included in the form of an extract thereof, a fraction thereof, or a processed product thereof. Additionally, the composition may include food additives that are foodologically acceptable in addition to the active ingredients.
본 발명에 있어서, "식품보조첨가제"란 식품에 보조적으로 첨가될 수 있는 구성요소를 의미하며, 각 제형의 건강기능식품을 제조하는데 첨가되는 것으로서 당업자가 적절히 선택하여 사용할 수 있다. 식품보조첨가제의 예로는 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 충진제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등이 포함되지만, 상기 예들에 의해 본 발명의 식품보조첨가제의 종류가 제한되는 것은 아니다.In the present invention, “food supplement” refers to a component that can be added to food as an auxiliary ingredient, and can be appropriately selected and used by a person skilled in the art as it is added to manufacture each type of health functional food. Examples of food supplements include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, and protective colloidal thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc., but the types of food supplements of the present invention are not limited to the above examples.
본 발명의 기능성 식품 조성물에는 건강기능식품이 포함될 수 있다. 본 발명에 있어서, "건강기능식품"이란 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 정제, 캅셀, 분말, 과립, 액상 및 환 등의 형태로 제조 및 가공한 식품을 말한다. 여기서 "기능성"이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건용도에 유용한 효과를 얻는 것을 의미한다. 본 발명의 건강기능식품은 당 업계에서 통상적으로 사용되는 방법에 의하여 제조 가능하며, 상기 제조시에는 당 업계에서 통상적으로 첨가하는 원료 및 성분을 첨가하여 제조할 수 있다. 또한 상기 건강기능식품의 제형 또한 건강기능식품으로 인정되는 제형이면 제한 없이 제조될 수 있다. 본 발명의 식품 조성물은 다양한 형태의 제형으로 제조될 수 있으며, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용 시 발생할 수 있는 부작용 등이 없는 장점이 있고, 휴대성이 뛰어나, 본 발명의 건강기능식품은 피부 미백, 피부 탄력 증진 또는 주름 개선, 또는 피부 보습, 항산화, 항염증 효과를 증진시키기 위한 보조제로 섭취가 가능하다.The functional food composition of the present invention may include health functional foods. In the present invention, “health functional food” refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills using raw materials or ingredients with functional properties useful to the human body. Here, “functionality” means controlling nutrients for the structure and function of the human body or obtaining useful effects for health purposes, such as physiological effects. The health functional food of the present invention can be manufactured by a method commonly used in the industry, and can be manufactured by adding raw materials and components commonly added in the industry. Additionally, the formulation of the health functional food can also be manufactured without limitation as long as it is a formulation recognized as a health functional food. The food composition of the present invention can be manufactured in various types of formulations, and unlike general drugs, it has the advantage of being made from food as a raw material and has no side effects that may occur when taking the drug for a long period of time, and is excellent in portability, making it suitable for use in the formulation of the present invention. Health functional foods can be taken as supplements to whiten skin, improve skin elasticity or improve wrinkles, or to improve skin moisturization, antioxidant, and anti-inflammatory effects.
본 발명의 건강기능식품이 취할 수 있는 형태에는 제한이 없으며, 통상적인 의미의 식품을 모두 포함할 수 있고, 기능성 식품 등 당업계에 알려진 용어와 혼용 가능하다. 아울러 본 발명의 건강기능식품은 당업자의 선택에 따라 식품에 포함될 수 있는 적절한 기타 보조성분과 공지의 첨가제를 혼합하여 제조할 수 있다. 첨가할 수 있는 식품의 예로는 육류, 소세지, 빵, 쵸코렛, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 본 발명에 따른 화학식 1로 표시되는 화합물을 주성분으로 하여 제조한 즙, 차, 젤리 및 주스 등에 첨가하여 제조할 수 있다. 또한 동물을 위한 사료로 이용되는 식품도 포함한다.There is no limit to the form that the health functional food of the present invention can take, and it can include all foods in the conventional sense and can be used interchangeably with terms known in the art, such as functional food. In addition, the health functional food of the present invention can be manufactured by mixing known additives with other appropriate auxiliary ingredients that can be included in the food according to the selection of a person skilled in the art. Examples of foods that can be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes, etc., and they can be prepared by adding the compound represented by Chemical Formula 1 according to the present invention to juices, teas, jellies, etc. prepared as main ingredients. It also includes foods used as feed for animals.
상기의 목적을 달성하기 위한 또 다른 양태로서, 본 발명은 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 의약외품 조성물을 제공한다.As another aspect for achieving the above object, the present invention provides a quasi-drug composition containing a mixed extract of lotus, cymbidium, and nasturtium as an active ingredient.
본 발명에서 상기 의약외품 조성물은 추라고 당류를 포함할 수 있다. 당류는 꿀, 로얄젤리, 설탕, 과당, 당알콜류, 프락토올리고탕 등이 사용되나 이에 제한되지 않고 당해 기술 분야에서 통상적으로 사용되는 물질이라면 어느 것이든 사용할 수 있다.In the present invention, the quasi-drug composition may contain sugars. Sugars include, but are not limited to, honey, royal jelly, sugar, fructose, sugar alcohols, and fructooligosaccharide, and any substance commonly used in the art can be used.
본 발명에서 상기 의약외품 조성물은 피부 미백, 피부 탄력증진 및 주름 개선, 피부 보습, 항산화, 항염증 등의 효과를 가질 수 있으나, 이에 제한되지 않는다. In the present invention, the quasi-drug composition may have effects such as skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory effects, but is not limited thereto.
상기 연꽃, 심비듐, 금련화, 추출물, 혼합 추출물, 꿀, 로얄젤리, 피부 미백, 피부 탄력증진 및 주름 개선, 피부 보습, 항산화, 항염증은 상기에서 설명한 바와 같다.The lotus flower, cymbidium, nasturtium, extract, mixed extract, honey, royal jelly, skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory are as described above.
본 발명의 의약외품 조성물에는 상기 성분 외에 필요에 따라 약학적으로 허용 가능한 담체, 부형제 또는 희석제를 더욱 포함할 수 있다. 상기 약학적으로 허용 가능한 담체, 부형제 또는 희석제는 본 발명의 효과를 해하지 않는 한 제한되지 않으며, 예를 들어 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제, 윤활제, 감미제, 방향제, 보존제 등을 포함할 수 있다.In addition to the above ingredients, the quasi-drug composition of the present invention may further include pharmaceutically acceptable carriers, excipients, or diluents as needed. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effect of the present invention, and includes, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. It can be included.
본 발명의 약학적으로 허용 가능한 담체, 부형제 또는 희석제의 대표적인 예로는, 락토즈, 덱스트로스, 슈크로스, 솔비톨, 만니톨, 자일리톨, 말티톨, 전분, 젤라틴, 글리세린, 아카시아 고무, 알지네이트, 칼슘포스페이트, 칼슘카보네이트, 칼슘실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트, 광물유, 프로필렌글리콜, 폴리에틸렌글리콜, 식물성 오일, 주사가능한 에스테르, 위텝솔, 마크로골, 트윈 61, 카카오지, 라우리지 등을 들 수 있다.Representative examples of pharmaceutically acceptable carriers, excipients or diluents of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium. Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl hydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil. , injectable esters, Wethepsol, Macrogol, Tween 61, Kakaoji, Lauridge, etc.
또한, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 조성물을 의약외품으로 사용하는 경우, 추가로 동일 또는 유사한 기능을 나타내는 유효성분을 1종 이상 함유할 수 있다. 예컨대, 공지의 피부 미백, 탄력 증진, 주름 개선 및 보습 성분을 포함할 수 있을 것이다. 추가적인 피부 미백, 탄력 증진, 주름 개선 및 보습 성분을 포함하게 되면 본 발명의 조성물의 피부 미백, 탄력 증진, 주름 개선 및 보습 효과는 더욱 증가될 수 있을 것이다. 상기 성분 추가 시에는 복합 사용에 따른 피부 안전성, 제형화의 용이성, 유효성분들의 안정성을 고려할 수 있다. 상기 의약외품 조성물은 당업계에 공지된 미백 성분으로서, 코즈산(Kojic acid), 알부틴(Arbutin) 등과 같은 티로시나제 효소활성을 억제하는 물질, 하이드로퀴논(Hydroquinone), 비타민-C(L-Ascorbic acid); 당업계에 공지된 피부 탄력, 주름 개선 또는 보습 성분으로서, 레티노산, TGF, 동물 태반 유래의 단백질, 베툴린산 및 클로렐라 추출물; 및 이들의 유도체와 각종 식물 추출물로 구성되는 군으로부터 선택되는 1종 또는 2종 이상의 성분을 추가로 포함할 수 있다. 추가 성분은 전체 조성물 중량에 대하여 0.0001 중량% 내지 10 중량%로 포함될 수 있을 것이며, 상기 함량 범위는 피부 안전성, 용이성 등의 요건에 따라 조절될 수 있을 것이다.In addition, when the composition containing the mixed extract of lotus, cymbidium, and nasturtium of the present invention as an active ingredient is used as a quasi-drug, it may additionally contain one or more active ingredients showing the same or similar functions. For example, it may contain known skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing ingredients. If additional skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing ingredients are included, the skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing effects of the composition of the present invention can be further increased. When adding the above ingredients, skin safety due to combined use, ease of formulation, and stability of the active ingredients can be taken into consideration. The quasi-drug composition includes whitening ingredients known in the art, such as substances that inhibit tyrosinase enzyme activity such as Kojic acid and Arbutin, Hydroquinone, Vitamin-C (L-Ascorbic acid); Skin elasticity, wrinkle improvement or moisturizing ingredients known in the art include retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract; and one or two or more ingredients selected from the group consisting of their derivatives and various plant extracts. Additional ingredients may be included in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety and ease of use.
본 발명의 의약외품 조성물은 소독 청결제, 샤워폼, 연고액, 물티슈, 코팅제 등을 예시할 수 있으나 이에 제한되는 것이 아니며, 의약외품의 제제화 방법, 용량, 이용방법, 구성성분 등은 기술분야에 공지된 통상의 기술로부터 적절히 선택될 수 있다.The quasi-drug composition of the present invention may include, but is not limited to, disinfectant cleaner, shower foam, ointment, wet tissue, coating agent, etc., and the formulation method, dosage, usage method, and components of the quasi-drug are commonly known in the technical field. It can be appropriately selected from the techniques of.
또한, 본 발명의 상기 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 의약외품 조성물은 개체의 피부에 도포하는 단계를 포함하는, 피부 미백용, 피부 탄력 증진 또는 주름 개선용, 또는 피부 보습 방법에 사용될 수 있다. 상기 개체는 쥐, 가축, 인간 등을 포함하는 포유동물을 제한 없이 포함한다.In addition, the quasi-drug composition containing the mixed extract of lotus, cymbidium, and nasturtium as an active ingredient of the present invention includes the step of applying it to the skin of an individual, for skin whitening, improving skin elasticity, or improving wrinkles, or for skin Can be used in moisturizing methods. The subject includes mammals, including rats, livestock, humans, etc., without limitation.
본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 화장료 조성물은 피부 미백, 탄력 증진 또는 주름 개선, 피부 보습, 항산화 및 항염 효과가 우수하다. 구체적으로 본 발명의 혼합 추출물을 포함하는 조성물은 멜라닌 총량 감소 효과를 나타내고 콜라겐 합성 촉진 효과 및 보습 효과가 우수하며, NO 생성 억제효과와 DPPH 라디칼 소거능이 단일 추출물에 비하여 우수하므로 피부 미백용, 탄력 증진용 또는 주름 개선용, 피부 보습용, 항산화용 및 항염증용 화장료 조성물로서의 활용도가 높다. 또한, 본 발명의 연꽃, 심비듐 및 금련화의 혼합 추출물을 유효성분으로 포함하는 조성물은 기능성 식품 조성물 및 의약외품 조성물로서 사용할 수 있다.The cosmetic composition containing the mixed extract of lotus, cymbidium, and nasturtium as an active ingredient of the present invention has excellent skin whitening, elasticity enhancement or wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory effects. Specifically, the composition containing the mixed extract of the present invention shows the effect of reducing the total amount of melanin, has an excellent effect of promoting collagen synthesis and moisturizing effect, and has an inhibitory effect on NO production and a DPPH radical scavenging effect compared to a single extract, so it is used for skin whitening and elasticity improvement. It has high utility as a cosmetic composition for wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory purposes. Additionally, the composition containing the mixed extract of lotus, cymbidium, and nasturtium of the present invention as an active ingredient can be used as a functional food composition and quasi-drug composition.
이하 본 발명을 하기 예에 의해 상세히 설명한다. 다만, 하기 예는 본 발명을 예시하기 위한 것일 뿐, 하기 예에 의해 본 발명의 범위가 제한되는 것은 아니다.Hereinafter, the present invention will be explained in detail by the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.
제조예Manufacturing example 1. 연꽃, 1. lotus flower, 심비듐cymbidium , , 금련화nasturtium 추출물의 제조 Preparation of extract
연꽃, 심비듐, 금련화를 물로 세척한 후, 세척된 연꽃, 심비듐, 금련화를 추출용기에 넣고 추출용매를 추가하여 상온에서 천천히 추출하였다. 상기 수득한 추출물을 거름종이 등을 이용하여 고형분을 제거하고 여과한 다음, 상기 추출액을 감압 농축하여 각각의 추출물을 제조하였다.After washing the lotus, cymbidium, and nasturtium with water, the washed lotus, cymbidium, and nasturtium were placed in an extraction container, and an extraction solvent was added to slowly extract them at room temperature. The obtained extract was filtered to remove solids using filter paper, etc., and then the extract was concentrated under reduced pressure to prepare each extract.
제조예Manufacturing example 2. 혼합 추출물의 제조 2. Preparation of mixed extract
제조예 1에서 제조된 연꽃, 심비듐, 금련화 각각의 단일 추출물을 동량으로 주입하여 혼합 추출물을 제조하였다. 각 혼합 추출물에는 미네랄 워터 또는 당류인 꿀 및/또는 로얄젤리를 추가하였다. 혼합 추출물의 제조 비율 예는 표 1에 나타내었다.A mixed extract was prepared by injecting equal amounts of the single extracts of lotus, cymbidium, and nasturtium prepared in Preparation Example 1. Mineral water or sugars such as honey and/or royal jelly were added to each mixed extract. An example of the preparation ratio of the mixed extract is shown in Table 1.
제조예 1의 각각의 추출물을 바탕으로 실시예 1 내지 4의 함량을 결정하였다. 실시예 1 내지 4의 경우, 각 추출물들이 대등한 비율로 혼합되어 협업작용으로 시너지 효과를 낼 수 있도록 최대 함량비를 조절한 것이다. The contents of Examples 1 to 4 were determined based on each extract of Preparation Example 1. In Examples 1 to 4, the maximum content ratio was adjusted so that each extract was mixed in equal proportions to produce a synergistic effect through collaborative action.
실험예Experiment example 1. 콜라겐 합성 증진 효과 1. Collagen synthesis enhancement effect
제조예 1에서 제조된 연꽃, 심비듐, 금련화 각각의 단일 추출물, 꿀, 로얄젤리 및 제조예 2에서 제조한 실시예 1 내지 4의 혼합 추출물에 대하여 다음과 같이 배양된 세포를 이용하여 콜라겐 합성 증진 효과를 측정하였다.Collagen synthesis using cells cultured as follows for the single extracts of lotus, cymbidium, and nasturtium prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2. The enhancement effect was measured.
키트 (procollagen type-1 C-peptide EIA kit, Takara, Japan)를 사용하여 프로 콜라겐의 합성을 측정하였다. HF(Human Fibroblast) 세포를 5 × 104세포/㎖의 밀도로 24 웰 플레이트에 넣고, 무첨가(대조군), 제조예 1에서 제조한 각각의 단일 추출물, 꿀, 로얄젤리 및 제조예 2에서 제조한 실시예 1 내지 4의 혼합 추출물 0.01 중량%를 넣고 37℃, 5% CO2 배양기에서 48시간 동안 배양하였다. 항-PIP 항체가 코팅된 (anti-PIP antibody-coated) 96 웰 플레이트에 항체-POD가 결합된 용액 (antibody-POD conjugate sol'n) 100㎕를 넣고 무첨가, 제조예 1에서 제조한 각각의 단일 추출물, 꿀, 로얄젤리 및 제조예 2에서 제조한 실시예 1 내지 4의 혼합 추출물에 표준액 20 ㎕를 첨가하고 알루미늄 호일로 차광시킨 후 3시간 동안 37°C에서 배양하였다.The synthesis of procollagen was measured using a kit (procollagen type-1 C-peptide EIA kit, Takara, Japan). HF (Human Fibroblast) cells were placed in a 24- well plate at a density of 5 0.01% by weight of the mixed extract of Examples 1 to 4 was added and cultured in an incubator at 37°C and 5% CO 2 for 48 hours. Add 100 ㎕ of antibody-POD conjugate sol'n to an anti-PIP antibody-coated 96 well plate, and add no additives, each single solution prepared in Preparation Example 1. 20 ㎕ of the standard solution was added to the extract, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, blocked from light with aluminum foil, and incubated at 37°C for 3 hours.
배양액을 제거하고 PBS로 4회 세척 후 말끔히 제거하고 기질용액 100 ㎕를 첨가한 후 실온에서 15분간 둔 후, H2SO4 100 ㎕를 첨가하여 가볍게 흔든 후, 450 nm에서 측정 흡광도를 측정하였다. 실험 결과는 표 2에 나타내었다. 모든 결과값은 3회 이상의 독립적인 실험을 거쳐 평균±표준편차(SD)로 나타내었다.The culture medium was removed, washed four times with PBS, thoroughly removed, 100 ㎕ of substrate solution was added, left at room temperature for 15 minutes, 100 ㎕ of H 2 SO 4 was added, shaken gently, and absorbance was measured at 450 nm. The experimental results are shown in Table 2. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.
그 결과, 상기 표 2에서 나타나듯이, 제조예 2에서 제조된 실시예 1의 혼합 추출물 처리 시, 제조예 1에서 제조된 각각의 단일 추출물보다 우수한 콜라겐 합성 증가 효과가 있음을 확인하였고, 제조예 2에서 제조된 실시예 2 내지 4의 혼합 추출물은 꿀 및/또는 로얄젤리에 의하여 실시예 1의 혼합 추출물보다 더욱 우수한 콜라겐 합성 증가 효과 있음을 확인하였다. As a result, as shown in Table 2, it was confirmed that the treatment of the mixed extract of Example 1 prepared in Preparation Example 2 had a better effect of increasing collagen synthesis than each single extract prepared in Preparation Example 1, and Preparation Example 2 It was confirmed that the mixed extracts of Examples 2 to 4 prepared in were more effective in increasing collagen synthesis than the mixed extract of Example 1 by honey and/or royal jelly.
실험예Experiment example 2. 멜라닌 생성 억제 효과 2. Melanin production inhibition effect
제조예 1에서 제조된 연꽃, 심비듐, 금련화 각각의 단일 추출물, 꿀, 로얄젤리 및 제조예 2에서 제조한 실시예 1 내지 4의 혼합 추출물에 대하여 미백 효과를 확인하기 위하여, 다음과 같이 배양된 세포를 이용하여 멜라닌 억제 효과를 측정하였다.In order to confirm the whitening effect of the single extracts of lotus, cymbidium, and nasturtium prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, culture was performed as follows. The melanin inhibition effect was measured using the cells.
구체적으로, 상기 시료의 미백 효능은 melanocyte내에 존재하는 멜라닌색소의 양이 감소하는 정도를 측정하여 평가하였다. 상기 시료를 0.01 중량%로 희석하여 B16 melanocyte에 처리하고 48시간 배양한 후, 멜라닌을 정량하여 미백 효능을 평가하였다. 멜라닌 함량은 총 단백질량 대비로 계산한 후, DMSO 대조군 기준 %로 구하였다. 양성 대조군은 Arbutin 100 ppm (Lim YJ et al., 2009, Arch Pharm Res.)을 사용하였다.Specifically, the whitening efficacy of the sample was evaluated by measuring the degree to which the amount of melanin pigment present in melanocytes was reduced. The sample was diluted to 0.01% by weight, treated with B16 melanocytes, cultured for 48 hours, and melanin was quantified to evaluate whitening efficacy. The melanin content was calculated based on the total protein amount and then calculated as a percentage based on the DMSO control group. Arbutin 100 ppm (Lim YJ et al., 2009, Arch Pharm Res.) was used as a positive control.
그 결과를 하기 표 3에 나타내었다. 모든 결과값은 3회 이상의 독립적인 실험을 거쳐 평균±표준편차(SD)로 나타내었다.The results are shown in Table 3 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.
그 결과, 상기 표 3에서 나타나듯이, 제조예 2에서 제조된 실시예 1의 혼합 추출물 처리 시, 제조예 1에서 제조된 각각의 단일 추출물보다 우수한 멜라닌 생성 억제 효과가 있음을 확인하였고, 제조예 2에서 제조된 실시예 2 내지 4의 혼합 추출물은 꿀 및/또는 로얄젤리에 의하여 실시예 1의 혼합 추출물보다 더욱 우수한 멜라닌 생성 억제 효과가 있음을 확인하였다. As a result, as shown in Table 3, it was confirmed that the treatment of the mixed extract of Example 1 prepared in Preparation Example 2 had a superior melanin production inhibition effect than each single extract prepared in Preparation Example 1, and Preparation Example 2 It was confirmed that the mixed extract of Examples 2 to 4 prepared in has a more excellent melanin production inhibition effect than the mixed extract of Example 1 by honey and/or royal jelly.
실험예Experiment example 3. 보습 인자 합성 요소 증진 효과 3. Moisturizing factor synthesis element enhancement effect
제조예 1에서 제조된 연꽃, 심비듐, 금련화 각각의 단일 추출물, 꿀, 로얄젤리 및 제조예 2에서 제조한 실시예 1 내지 4의 혼합 추출물에 대하여 보습 인자 합성 요소 증진 효과를 확인하기 위하여, 다음과 같이 배양된 세포를 이용하여 피부 보습 효과를 측정하였다.In order to confirm the effect of enhancing moisturizing factor synthesis elements for the single extracts of lotus, cymbidium, and nasturtium prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, The skin moisturizing effect was measured using cultured cells as follows.
구체적으로, 37℃, 5% CO2의 조건에서 10% FBS(Gibco), 스트렙토마이신(streptomycin) 100 ㎍/㎖, 페니실린 (penicillin) 100 ㎍/㎖, 암포테리신B (amphotericin B) 0.25 ㎍/㎖가 첨가된 DMEM 배지를 사용하여 각질형성세포(HaCaT, Human keratinocyte)를 증식시켰다. Specifically, under conditions of 37°C and 5% CO 2 , 10% FBS (Gibco), streptomycin 100 ㎍/㎖, penicillin 100 ㎍/㎖, amphotericin B 0.25 ㎍/㎖. Keratinocytes (HaCaT, human keratinocytes) were proliferated using DMEM medium supplemented with ㎖.
형성된 각질형성세포(HaCaT, Human keratinocyte)를 6웰에 5 X 105cell/well의 밀도로 3ml의 배지와 함께 분주한 뒤, 37℃, 5% CO2 배양기에서 24시간 배양하였다. 배양된 상기 배지는 PBS로 씻어 FBS를 제거하였다. FBS가 제거된 배지에 무처리, 제조예 1에서 제조된 장미, 작약, 아이리스 각각의 단일 추출물, 꿀, 로얄젤리 및 제조예 2에서 제조한 실시예 1, 2, 3, 4를 0.01 중량%로 각각 3ml씩 처리한 후 다시 배양하고, HAS3(Hyaluronan Synthases 3) mRNA양을 비교하였다. RNA는 RNA추출 키트(RNeasy Mini Kit, Qiagen, USA)를 사용하였고, cDNA는 수퍼스크립트 II 역전사효소(SuperScript II reverse transcriptase (Invitrogen, USA))를 이용하여 합성하였으며, 플레티넘 PCR 수퍼믹스 (Platinum PCR supermix (Invitrogen, USA))와 제작된 프라이머를 사용하여 PCR을 수행하였다. cDNA 합성은 1㎍의 총 RNA(total RNA)로 50℃에서 1시간, 85℃에서 5분 동안 열을 가한 후, 반응을 중지시켰다. PCR 증폭은 94℃에서 50초 동안 변성시키고 60℃에서 50초 동안 어닐링하고 72℃에서 50초 동안 익스텐션시켰다. 이를 26 싸이클(cycle) 반복하고 마지막 익스텐션은 72℃에서 5분간 수행하였고, 증폭된 결과물은 2% 아가로스 겔에서 전기영동하여 유전자 발현을 퓨전 Fx5 검출 시스템 (Fusion Fx5 detection system (VilberLourmat, Germany))으로 확인하였다.The formed keratinocytes ( HaCaT , human keratinocytes) were dispensed into 6 wells at a density of 5 The cultured medium was washed with PBS to remove FBS. To the medium from which FBS was removed, untreated, single extracts of rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly, and Examples 1, 2, 3, and 4 prepared in Preparation Example 2 were added to 0.01% by weight. After treating each with 3 ml, they were cultured again, and the amount of HAS3 (Hyaluronan Synthases 3) mRNA was compared. RNA was synthesized using an RNA extraction kit (RNeasy Mini Kit, Qiagen, USA), cDNA was synthesized using SuperScript II reverse transcriptase (Invitrogen, USA), and Platinum PCR supermix. (Invitrogen, USA)) and PCR was performed using the prepared primers. For cDNA synthesis, 1 μg of total RNA was heated at 50°C for 1 hour and 85°C for 5 minutes, and then the reaction was stopped. PCR amplification consisted of denaturation at 94°C for 50 seconds, annealing at 60°C for 50 seconds, and extension at 72°C for 50 seconds. This was repeated for 26 cycles, and the final extension was performed at 72°C for 5 minutes, and the amplified product was electrophoresed on a 2% agarose gel to determine gene expression using the Fusion Fx5 detection system (VilberLourmat, Germany). It was confirmed.
그 결과를 하기 표 4에 나타내었다. 모든 결과값은 3회 이상의 독립적인 실험을 거쳐 평균±표준편차(SD)로 나타내었다.The results are shown in Table 4 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.
그 결과, 상기 표 4에서 나타나듯이, 제조예 2에서 제조된 실시예 1의 혼합 추출물 처리 시, 제조예 1에서 제조된 각각의 단일 추출물보다 HAS 발현이 증가함을 확인하였고, 제조예 2에서 제조된 실시예 2 내지 4의 혼합 추출물은 꿀 및/또는 로얄젤리에 의하여 실시예 1의 혼합 추출물보다 HAS 발현이 더욱 증가함을 확인하였다. As a result, as shown in Table 4, it was confirmed that when processing the mixed extract of Example 1 prepared in Preparation Example 2, HAS expression increased compared to each single extract prepared in Preparation Example 1. It was confirmed that the mixed extract of Examples 2 to 4 increased HAS expression more than the mixed extract of Example 1 by honey and/or royal jelly.
실험예Experiment example 4. 항산화 효과 4. Antioxidant effect
제조예 1에서 제조된 연꽃, 심비듐, 금련화 각각의 단일 추출물, 꿀, 로얄젤리 및 제조예 2에서 제조한 실시예 1 내지 4의 혼합 추출물에 대하여 항산화 효과를 확인하기 위하여, 다음과 같이 배양된 세포를 이용하여 피부 항산화 효과를 측정하였다. In order to confirm the antioxidant effect of the single extracts of lotus, cymbidium, and nasturtium prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, culture was performed as follows. The skin antioxidant effect was measured using the cells.
구체적으로, 상기 시료의 라디칼 소거능은 DPPH에 존재하는 라디칼이 소거될 때, DPPH가 보라색에서 노란색으로 변화하는 것을 이용하여 평가하였다. 대조군으로는 Vit C를 사용하였다. 상기 시료를 메탄올에 녹인 후 이용하였으며 실험방법은 0.15 mM DPPH 용액과 시료를 100㎕씩 혼합하여 상온에서 30분 반응시킨 후, DPPH의 색의 변화를 540 nm에서 흡광도(A540)를 측정하였다. A540 값을 통해 IC50값을 구하여 라디칼 소거능을 비교 평가하였다. Specifically, the radical scavenging ability of the sample was evaluated using the change of DPPH from purple to yellow when the radicals present in DPPH are scavenged. Vit C was used as a control. The sample was dissolved in methanol and then used. The experimental method was to mix 100 μl of each sample with a 0.15 mM DPPH solution and react at room temperature for 30 minutes, and then measure the change in color of DPPH by measuring the absorbance (A540) at 540 nm. The IC 50 value was obtained from the A540 value to compare and evaluate the radical scavenging ability.
그 결과를 하기 표 5에 나타내었다. 모든 결과값은 3회 이상의 독립적인 실험을 거쳐 평균±표준편차(SD)로 나타내었다.The results are shown in Table 5 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.
그 결과, 상기 표 5에서 나타나듯이, 제조예 2에서 제조된 실시예 1의 혼합 추출물 처리 시, 제조예 1에서 제조된 각각의 단일 추출물보다 우수한 항산화 효과가 있음을 확인하였고, 제조예 2에서 제조된 실시예 2 내지 4의 혼합 추출물은 꿀 및/또는 로얄젤리에 의하여 실시예 1의 혼합 추출물보다 더욱 우수한 항산화 효과가 있음을 확인하였다. As a result, as shown in Table 5, it was confirmed that the mixed extract of Example 1 prepared in Preparation Example 2 had a superior antioxidant effect than each single extract prepared in Preparation Example 1. It was confirmed that the mixed extract of Examples 2 to 4 had a better antioxidant effect than the mixed extract of Example 1 due to honey and/or royal jelly.
실험예Experiment example 5. 항염 효과 5. Anti-inflammatory effect
제조예 1에서 제조된 연꽃, 심비듐, 금련화 각각의 단일 추출물, 꿀, 로얄젤리 및 제조예 2에서 제조한 실시예 1 내지 4의 혼합 추출물에 대하여 항염 효과를 확인하기 위하여, 다음과 같이 배양된 세포를 이용하여 피부 항염 효과를 측정하였다.In order to confirm the anti-inflammatory effect of the single extracts of lotus, cymbidium, and nasturtium prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, culture was performed as follows. The skin anti-inflammatory effect was measured using the cells.
구체적으로, Raw 264.7 cell을 이용하여 상기 추출물들의 NO 생성 억제 효과를 측정하였다. 양성 대조군으로는 20 mg/ml L-NMMA1 (NOS 억제제)를 사용했으며 상기 시료를 0.01 중량% 농도로 희석하여 30분 전 처리 후, 1 μg/ml LPS를 처리하고 24시간 배양한 후 NO 정량 Kit를 이용하여 NO 생성 억제능을 아래와 같이 평가하였다.Specifically, the NO production inhibition effect of the extracts was measured using Raw 264.7 cells. As a positive control, 20 mg/ml L-NMMA1 (NOS inhibitor) was used, and the sample was diluted to a concentration of 0.01% by weight, treated 30 minutes ago, treated with 1 μg/ml LPS, and cultured for 24 hours using the NO Quantification Kit. The ability to inhibit NO production was evaluated as follows.
그 결과를 하기 표 6에 나타내었다. 모든 결과값은 3회 이상의 독립적인 실험을 거쳐 평균±표준편차(SD)로 나타내었다.The results are shown in Table 6 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.
그 결과, 상기 표 6 나타나듯이, 제조예 2에서 제조된 실시예 1의 혼합 추출물 처리 시, 제조예 1에서 제조된 각각의 단일 추출물보다 높은 NO 생성 억제능을 확인하였고, 제조예 2에서 제조된 실시예 2 내지 4의 혼합 추출물은 꿀 및/또는 로얄젤리에 의하여 실시예 1의 혼합 추출물보다 더욱 높은 NO 생성 억제능을 확인하였다. As a result, as shown in Table 6 above, when processing the mixed extract of Example 1 prepared in Preparation Example 2, it was confirmed that the NO production inhibition ability was higher than that of each single extract prepared in Preparation Example 1, and the The mixed extracts of Examples 2 to 4 were confirmed to have a higher NO production inhibition ability than the mixed extract of Example 1 due to honey and/or royal jelly.
즉, 본 발명에 따른 혼합 추출물(실시예 1)은 각각의 연꽃, 심비듐, 금련화 단일 추출물보다 우수한 시너지 작용을 하는 것을 알 수 있으며, 실시예 2 내지 4를 통해 꿀 및/또는 로얄젤리가 첨가된 경우, 혼합 추출물의 시너지 작용이 더 높아짐을 알 수 있다. In other words, it can be seen that the mixed extract according to the present invention (Example 1) has a superior synergistic effect than the individual lotus, cymbidium, and nasturtium single extracts, and through Examples 2 to 4, honey and/or royal jelly When added, it can be seen that the synergistic effect of the mixed extract is further increased.
이러한 결과를 통해 상기 혼합 추출물이 피부 미백, 피부 탄력 증진 또는 주름 개선, 피부 보습, 피부 노화 방지, 항산화, 및 피부 염증 개선 효과에 매우 효과적임을 알 수 있다.These results show that the mixed extract is very effective in skin whitening, improving skin elasticity or wrinkles, moisturizing skin, preventing skin aging, antioxidant, and improving skin inflammation.
이상의 설명으로부터, 본 발명이 속하는 기술분야의 당업자는 본 발명이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 이와 관련하여, 이상에서 기술한 실시 예들은 모든 면에서 예시적인 것이며 한정적인 것이 아닌 것으로서 이해해야만 한다. 본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허 청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed as including the meaning and scope of the patent claims described below rather than the detailed description above, and all changes or modified forms derived from the equivalent concept thereof are included in the scope of the present invention.
Claims (13)
A cosmetic composition for skin whitening containing mixed extracts of lotus, cymbidium, and nasturtium as active ingredients.
A cosmetic composition for improving skin elasticity or improving wrinkles, comprising mixed extracts of lotus, cymbidium, and nasturtium as active ingredients.
A cosmetic composition for moisturizing skin containing mixed extracts of lotus, cymbidium, and nasturtium as active ingredients.
An antioxidant cosmetic composition containing mixed extracts of lotus, cymbidium, and nasturtium as active ingredients.
An anti-inflammatory cosmetic composition containing mixed extracts of lotus, cymbidium, and nasturtium as active ingredients.
상기 기능성 식품 조성물은 피부 미백용, 피부 탄력 증진 또는 주름 개선용, 피부 보습용, 항산화용 또는 항염증용인 것인, 기능성 식품 조성물.
A functional food composition containing mixed extracts of lotus, cymbidium, and nasturtium as active ingredients,
The functional food composition is for skin whitening, improving skin elasticity or wrinkles, moisturizing the skin, antioxidant or anti-inflammatory.
꿀, 로얄젤리, 또는 둘 모두를 추가로 포함하는, 기능성 식품 조성물.
According to clause 8,
A functional food composition further comprising honey, royal jelly, or both.
상기 의약외품 조성물은 피부 미백용, 피부 탄력 증진 또는 주름 개선용, 피부 보습용, 항산화용 또는 항염증용인 것인, 의약외품 조성물.
A quasi-drug composition containing mixed extracts of lotus, cymbidium, and nasturtium as active ingredients,
The quasi-drug composition is for skin whitening, improving skin elasticity or wrinkles, moisturizing the skin, antioxidant, or anti-inflammatory.
꿀, 로얄젤리, 또는 둘 모두를 추가로 포함하는, 의약외품 조성물.
According to clause 11,
A quasi-drug composition further comprising honey, royal jelly, or both.
꿀, 로얄젤리, 또는 둘 모두를 추가로 포함하는, 화장료 조성물.
According to any one of claims 3 to 7,
A cosmetic composition further comprising honey, royal jelly, or both.
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