KR20170136957A - Composition for skin improvement comprising multiple extract - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising mixed extracts of Nelumbo nucifera, Cymbidium hybida, and Tropaeolum majus as active ingredients, and more particularly, to a cosmetic composition having effects of skin whitening, skin elasticity improvement, wrinkle reduction, skin moisturizing, antioxidation, anti-inflammation, and the like. The composition of the present invention is excellent in anti-inflammatory and antioxidative effects, exhibits an effect of reducing the total amount of melanin, and has excellent collagen synthesis promoting effect, and a moisturizing effect, thereby being used as a cosmetic composition for skin whitening, elasticity improvement or wrinkle reduction, and skin moisturizing. In addition, the composition comprising the mixed extract of Nelumbo nucifera, Cymbidium hybida, and Tropaeolum majus of the present invention can be used as a functional food composition and a quasi-drug composition.

Description

[0001] The present invention relates to a cosmetic composition comprising a mixed extract,

The present invention relates to a cosmetic composition comprising a mixed extract of lotus flower, simvidium and azalea. More specifically, the present invention relates to a cosmetic composition comprising the mixed extract as an active ingredient, skin whitening, skin elasticity enhancement, wrinkle improvement, skin moisturizing, And the like. The present invention also relates to a functional food composition and a quasi-drug composition comprising a mixed extract of lotus flower, simvidium and algae.

Commonly known whitening ingredients include substances inhibiting tyrosinase enzyme activity such as kojic acid or arbutin, hydroquinone, vitamin C (L-ascorbic acid) or derivatives thereof, various plant extracts . By inhibiting the synthesis of melanin pigment, they can brighten the skin tone to realize skin whitening, and it is possible to improve skin hypercholesterolemia such as stain or freckles due to ultraviolet rays, hormones or heredity. However, when applied to skin, there is a problem that the use amount is limited due to safety problems such as irritation and redness, or the effect is insignificant, so that a practical effect can not be expected.

Collagen is a major substrate protein produced in the fibroblasts of the skin and exists in extracellular epilepsy. Its important functions are mechanical durability of the skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, cell division and differentiation (Induction of growth of an organism or wound healing) are known. Such collagen is reduced by photoaging by age and ultraviolet irradiation, and collagenase activity, which degrades collagen, promotes collagen reduction. This is known to be closely related to the formation of wrinkles in the skin.

In addition, collagen is a substrate protein that plays an important role in moisture retention in the dermal layer. These protein proteins adsorb moisture and increase the water holding capacity inside the structure, thereby enabling the skin to maintain proper moisture content. Is known to be involved in maintaining the elasticity of the skin.

At present, retinoids, adenosine, animal placenta-derived proteins, chlorella extract, and the like are known as cosmetics for improving skin elasticity and improving wrinkles. The best known retinol is a substance that promotes collagen synthesis, but it is unstable. When applied to the skin, there is a limitation in usage due to safety problems such as irritation and redness. Chlorella extract has little effect and thus has substantially improved skin elasticity and wrinkle It is known that it is difficult to expect.

Although the human body has a balance of the oxidation promoting substance and the oxidation inhibiting substance, when the balance state is lost due to various factors and is inclined to promote the oxidation, oxidative stress is induced in the living body, Leading to pathological disease. Reactive oxygen species (ROS), which is a direct cause of oxidative stress, are chemically unstable and highly reactive, and can easily react with various biomaterials such as DNA, protein, lipid and carbohydrate. Attacks, causing irreversible damage to cells and tissues, or causing mutations, cytotoxicity, and cancer.

In the human body, macrophages respond to pathogens and cause inflammation such as tumor necrotic factor-α, TNF-α, interleukin-6 (IL-6), and interleukin-1β (NO) and prostaglandins (prostaglandin E2, PGE2) by synthesizing inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) ). Physiologically, NO plays a variety of roles such as removing bacteria and tumors, regulating blood pressure, or mediating neurotransmission. However, when the inflammatory reaction occurs, the expression of iNOS is increased in related cells, and a large amount of NO is produced. Excessively produced NO causes tissue damage, gene mutation, nerve damage, etc., And promote the inflammatory response.

In the inflammatory reaction, various free radicals are produced along with various inflammation inducers. Normal free radicals are involved in maintaining the homeostasis of cells and affect cell differentiation, growth, survival, and aging. These reactive oxygen species (ROS) in the free radicals are produced constantly through oxidation and reduction of oxygen by respiration and immune reaction in intracellular mitochondria. Harmful ROS are usually caused by superoxide dismutase, catalase, glutathione peroxidase, glutamine reductase, vitamin C, vitamin E, uric acid, bilirubin, It is eliminated by the action of the same antioxidant system. However, when the balance of generation and elimination of free radicals is collapsed, oxidative stress occurs, which causes various pathological changes such as inflammation, aging, or cancer. In fact, it has been confirmed that oxidative stress is increased in many clinical diseases. Various studies on new antioxidants have been made to reduce such oxidative stress.

Studies on the environmentally friendly and harmless components that are eco-friendly and protect the skin from environmental stimuli, stress, and chemical treatments due to various causes are continuing.

As described above, the above-mentioned cosmetic compositions have problems such as giving an economic burden and suffering to people, causing side effects, and using only temporary or fractional pharmacological mechanism, so that the effect is more excellent and continuous, There is a growing demand for compositions without side effects.

Under these circumstances, the present inventors have conducted studies on substances having excellent skin whitening, skin elasticity enhancement, wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory effects, and accordingly, compositions containing a mixed extract of rose, Melanin synthesis, promoting collagen synthesis, moisturizing, anti-inflammation and antioxidative effects, and has completed the cosmetic composition, the functional food composition and the quasi-drug composition utilizing the melanin.

Korean Patent Publication No. 10-2014-0006139 Korean Patent No. 10-1638538

The main object of the present invention is to provide a cosmetic composition comprising as an active ingredient a mixed extract of lotus flower, simvidium and azalea.

It is another object of the present invention to provide a functional food composition comprising a mixed extract of lotus flower, simvidium, and ganoderma as an active ingredient.

It is still another object of the present invention to provide a quasi-drug composition comprising a mixed extract of lotus flower, simvidium and algae as an active ingredient.

To achieve the above object, one aspect of the present invention provides a cosmetic composition comprising as an active ingredient, a mixed extract of lotus flower, simvidium, and azalea.

The term "lotus (Nelumbo nucifera Gaertn)" as used in the present invention is a flower that has been loved by the imperial family so that there is a "lotus flower garden" where the royal family nobles, both young and old, Kite is also used as a very useful herb medicine in Oriental medicine. It uses most of the kite from fruit to leaves, flowers, rice balls, pistil, and roots. The most commonly used part is fruit, which helps the energy, protects the five, and eliminates thirst and diarrhea. Especially, it is a medicinal substance that is useful for modern people who have sensitive nerves because they have the effect of stabilizing their minds. Leaves with a bitter taste while moisturizing the blood circulation and good to loosen the eolyeo, bruise caused by congestion, and the wound, if you stick to the wounds and hemostatic effect is great. It is also known as a flower that is good for skin beauty since ancient times, as it is described as the best flower that can manage the skin in 'Baekwol-dongjeonjeon' which can be called secretary of skin beauty and beauty.

The term "Cymbidium ", as used in the present invention, is an ovary which has been regarded as a symbol of the military for 2000 years. It is also one of the birthplaces that have a strong fragrance and are beautiful and saved from the past. It has a form and a fragrance of flower which is called 'noble flower', and it is used to make the water soft and supple by using flower which has traditionally recognized beauty effect. Especially, It is known to be used as a recipe for beauty.

The term " Tropaeolum majus L. "used in the present invention is called a golden lotus flower that resembles a lotus leaf but grows on the ground. In the sixteenth century in the United Kingdom, Geilian painting was considered one of the treasures and was one of the precious treasures that the Spanish conquerors brought from Europe with the gold from the Peruvian Inca to Europe. It is known as a precious ingredient used as a material for the health of the European royal family. It is known that it is cold and has no taste. It is known to be effective in reducing fever and also effective in acute conjunctivitis.

"Extract" in the present invention means a liquid component obtained by immersing a desired substance in various solvents and then extracting it at a room temperature, a low temperature, or a warmed state for a predetermined time, a liquid component obtained by removing the solvent from the liquid component Means a product such as a solid. In addition, in addition to the above-mentioned results, the present invention can be broadly interpreted to include all of the diluted solution, the concentrated solution thereof, the adjusted product thereof, and the purified product.

In the present invention, the extract means a lotus flower, a simvidium extract, and a gingko extract. The lotus, simvidium and alfalfa extracts can be extracted from various organs of natural, hybrid, and variant plants, and can be extracted from various organs such as roots, loose bodies, stems, leaves, flowers, Extractable. The lotus flower, symbium, and gold leaf extract can be obtained by extraction with water or various organic solvents. The organic solvent to be used may be water, a polar solvent or a nonpolar solvent, and more specifically, water, a lower alcohol having 1 to 6 carbon atoms (for example, Methanol, ethanol, propanol or butanol), or a mixed solvent thereof, most specifically, methanol or a mixture thereof. In addition, a saccharide may be contained as a solvent for the extract in the present invention. Specific examples of the saccharides include, but are not limited to, honey, royal jelly, sugar, fructose, sugar alcohols, fructooligosaccharides and the like.

As the method for obtaining the extract, there is no particular limitation as long as it is possible to obtain a lotus flower, a simvidium flower, and a gold flower extract. Specifically, the lotus flower, the symbium, the roots, the stem, the leaf, Methods such as a cold extraction method in which flowers, dried products, and processed products thereof are immersed in the solvent and extracted at room temperature, a heat extraction method, an ultrasonic extraction method in which ultrasonic waves are extracted, and a reflux extraction method using a reflux condenser.

The term "mixed extract " used in the present invention may be an extract obtained by mixing extracts of each component of lotus flower, simvidium, and ghaylini at an appropriate weight ratio, and may be an extract of lotus flower, simvidium, It may be mixed and extracted. It has been confirmed that the mixed extract is more effective for skin whitening, skin elasticity enhancement or wrinkle improvement, skin moisturizing, skin aging prevention, antioxidation, and skin inflammation improvement than each lotus flower, simvidium and monodisperse single extract, .

The weight ratio of the lotus extract, simvidium extract and sesame seed extract to the weight ratio (w / w) of 1 to 25: 1 to 25: 1 to 25, specifically 1: 15: 1 to 15: w / w), more specifically from 1: 5: 1 to 5: 1 to 5, and may have an equivalent weight ratio.

In the present invention, a saccharide may be added to the mixed extract. Examples of the saccharides include honey, royal jelly, sugar, fructose, sugar alcohols, fructooligosaccharides, and the like, but not limited thereto, and any substance conventionally used in the art can be used. More specifically, as a saccharide, honey and royal jelly can be used.

As used herein, the term "honey" means that bees are collected from the nectar of flowers and stored as food. Honey is divided into native honey and bee honey depending on the kind of bee, and it is called acacia honey, honey honey, honey honey, honey, buckwheat honey etc. depending on the flowers and the color and taste are different according to the kinds of flowers. About 80% of honey is carbohydrate, 36 ~ 38% of fructose, 34 ~ 36% of glucose, and 2 ~ 3% of sugar and dextrin. Other ingredients include formic acid, lactic acid, malic acid, pigment, gum, wax, enzymes, etc. in addition to 0.2 to 0.3% protein, 0.05 to 0.2% ash, vitamin B complexes B1, B2 and B6 and pantothenic acid. In Korea, honey is widely used for edible and medicinal purposes, and is also used as a sweetener.

The term "royal jelly" as used in the present invention means the food of the queen bee as a gift to the king of the worker bees. Royal jelly contains protein, fat, carbohydrates and mysterious nutrients containing various trace elements, and R is known to have various effects. Royal jelly is also known to be effective in skin care and nutrition.

According to one embodiment of the present invention, when a saccharide such as honey or royal jelly is added to a mixed extract of lotus flower, simvidium, and yuzen, collagen synthesis is reduced as compared with a single extract of lotus flower, HAS (Hyaluronan Synthases) expression was increased, and radical scavenging ability and NO production were inhibited.

Thus, when a saccharide such as honey and / or royal jelly is added to a mixed extract of lotus flower, simvidium and gilt blossom, a synergistic action with the extract is obtained, which is superior to skin whitening, skin elasticity, skin moisturizing, anti- As shown in Fig.

In the present invention, the cosmetic composition may have effects such as skin whitening, skin elasticity enhancement and wrinkle improvement, skin moisturizing, antioxidant, anti-inflammation, and the like, but is not limited thereto.

The term "skin whitening" in the present invention means not only brightening the skin tone by inhibiting the synthesis of the melanin pigment but also improving hyperpigmentation of skin such as stain or freckles due to ultraviolet rays, hormones or heredity.

According to one specific embodiment, melanoma cells are treated with a composition comprising the lotus flower, the simvidium, and the ginseng mixture extract of the present invention or the mixed extract of lotus flower, the simvidium and the ginseng with honey and / or royal jelly, The results showed that the mixed extract had a better melanin production inhibitory effect on melanoma cells as compared with the control or single extracts, and thus it could be used for skin whitening purposes (Table 3).

Thus, when honey and / or royal jelly is added to a mixed extract of lotus flower, simvidium and gilt blossom or a mixed extract of lotus flower, symbium and gilt blossom, synergistic action is caused as compared with a single extract, The results are summarized as follows.

The term "skin elasticity enhancement" in the present invention is intended to alleviate the degree to which the skin is stuck or stretched. Elastic fibers composed of elastin are present together with collagen fibers called collagen, The skin elasticity is maintained. In the present invention, "wrinkle improvement" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already formed wrinkles.

According to a specific embodiment, HF (Human Fibroblast) cells treated with a composition of lotus, simvidium and azalea blended extract of the present invention or a composition obtained by adding lotus, simvidium and gilt blend to honey or royal jelly are cultured As a result of measuring the synthesis of procollagen, it was confirmed that the collagen synthesis effect was the best in the mixed extract (Table 2).

Thus, when honey and / or royal jelly are added to a mixed extract of lotus flower, simvidium and gilt blossom or a mixed extract of lotus flower, simvidium and gilt blossom, synergistic action is caused as compared with a single extract, It was found that it had excellent effect.

In the present invention, "skin moisturizing" means increasing the skin's moisture content and maintaining a moist state. Skin moisturizing effect can help to improve wrinkles and elasticity of skin.

According to a specific embodiment, a composition comprising a lotus flower, a simvidium and an ointment blended extract of the present invention or a mixed extract of lotus flower, simvidium, and gilt blend with honey and / or royal jelly is added to a keratinocyte (HaCaT, Human keratinocyte ), And the amount of HAS3 mRNA was compared with that of the control. As a result, it was confirmed that HAS3 mRNA expression was increased in the case of treatment with the mixed extract as compared with the control and single extract (Table 4).

These results indicate that the mixed extract can exhibit skin moisturizing effect.

As a result, when honey and / or royal jelly are added to a mixed extract of lotus flower, simvidium and gilt blossom or a mixed extract of lotus flower, simvidium and gilt blossom, synergistic action is caused as compared to a single extract, The results are summarized as follows.

The term "antioxidant" in the present invention means an oxidation-inhibiting action, and the human body has a balance between a prooxidant and an antioxidant. However, due to various factors, And leaning towards oxidative stimulation leads to oxidative stress leading to potential cellular damage and pathological disease. Reactive oxygen species (ROS), which is a direct cause of oxidative stress, is unstable and highly reactive. It reacts easily with various bio-materials, attacks irreversible damage to cells and tissues, Cytotoxicity and carcinogenesis. Active nitrogen species (RNS) such as NO, HNO ₂ , and ONOO ^- are produced by the immune response of macrophage neutrophils and other immune cells during inflammatory reaction, and ROS is also produced. Such active oxygen oxidizes and destroys cells in the body, thereby exposing them to various diseases. Therefore, when the mixed extract of the present invention is contained in cosmetics, the antioxidative effect can be achieved, thereby contributing to health promotion.

According to a specific example, the DPPH radical scavenging ability (Table 5) of the lotus flower, simvidium and gilt blend extract of the present invention was analyzed to confirm that the extract had strong antioxidant ability. It was confirmed that the IC ₅₀ value of the mixed extract was lower than that of the single extract. These results indicate that the mixed extract can exhibit antioxidative effects.

Thus, when honey and / or royal jelly are added to a mixed extract of lotus flower, simvidium and gilt blossom or a mixed extract of lotus flower, simvidium and gilt blossom, synergistic action is produced as compared with a single extract, As shown in Fig.

The term "anti-inflammatory" in the present invention means an action to suppress inflammation. It is known that the control of the inflammatory reaction is extremely complicated, which is known to reduce the enhancement and damage of the in vivo restoration system. However, if the inflammatory response is sustained by repeated tissue damage or regeneration, ROS and RNS are overproduced in inflammation-related cells, resulting in permanent gene deformation. Thus, ROS and RNS are deeply involved in the inflammatory response that regulates the action of various cells in vivo. During inflammation, large amounts of proinflammatory cytokines, nitric oxide (NO), and prostaglandin (prostaglandin E2, PGE2) are induced by inducible nitric oxide synthase (iNOS) and cyclooxygenase (cyclooxygenase-2, COX-2). The inflammation is a cause of various inflammatory diseases. The composition comprising the lotus flower, symbium, and ginseng extract of the present invention may have various inflammatory diseases prevention and improvement effects through anti-inflammatory action.

According to one embodiment of the present invention, the inhibitory effect on the NO production of the lotus flower, the simvidium, and the mutant extract of the present invention was examined. As a result, Bar, and the mixed extract had a strong anti-inflammatory effect (Table 6).

Thus, when honey and / or royal jelly are added to a mixed extract of lotus, simvidium and gilt blossom or a mixed extract of lotus, simvidium and gilt blossom, synergistic action is caused as compared with a single extract, The results are summarized as follows.

The cosmetic composition according to the present invention can be used as a cosmetic composition in the form of a solution, an ointment for external use, a cream, a foam, a nutritional lotion, a softening water, a pack, a soft water, an emulsion, a makeup base, But are not limited to, emulsions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays It is not.

In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a general skin cosmetic composition, and examples thereof include oil, water, a surfactant, a moisturizer, A thickening agent, a chelating agent, a coloring matter, an antiseptic, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier to be contained in the cosmetic composition of the present invention varies depending on the formulations.

When the formulation of the present invention is an ointment, a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Mixtures of these may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil may be used, in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycols or sorbitan may be used have.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, .

The cosmetic composition of the present invention can be used not only to contain a honey extract or a royal jelly saccharide in addition to the mixed extract of Lotus, Symbidium and Araliae or a mixed extract of Lotus, Symbidium and Araliae, Preservatives, antioxidants, solvents, fragrances, fillers, blocking agents, pigments, absorbers and dyes, and the like.

According to another aspect of the present invention, there is provided a functional food composition comprising, as an active ingredient, a mixed extract of lotus flower, simvidium, and azalea.

In the present invention, the functional food composition may further include saccharides. Examples of the saccharides include honey, royal jelly, sugar, fructose, sugar alcohols, fructooligosaccharides, and the like, but not limited thereto, and any substance conventionally used in the art can be used.

In the present invention, the functional food composition may have effects such as skin whitening, skin elasticity enhancement and wrinkle improvement, skin moisturizing, antioxidant, anti-inflammation, and the like, but is not limited thereto.

The lotion, simvidium, algae, extract, mixed extract, honey, royal jelly, skin whitening, skin elasticity enhancement and wrinkle improvement, skin moisturizing, antioxidant, antiinflammation are as described above. The functional food composition may be used in the form of a health functional food, but is not limited thereto.

The functional food composition comprising the lotus flower and the mixed extract of ganoderma as an active ingredient contained in the functional food composition of the present invention may be contained in the form of an extract thereof, a fraction thereof or a processed product thereof. The composition may also include a food-acceptable food-aid additive in addition to the active ingredient.

In the present invention, the term "food-aid additive " means a component that can be added to foods in a supplementary manner, and is appropriately selected and used by those skilled in the art as added to produce health functional foods of each formulation. Examples of food-aid additives include flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, , a pH adjusting agent, a stabilizer, a preservative, a glycerin, an alcohol, and a carbonating agent used in a carbonated drink. However, the types of the food auxiliary additives of the present invention are not limited by these examples.

The functional food composition of the present invention may include a health functional food. In the present invention, the term "health functional food" refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and rings using raw materials and components having useful functions in the human body. The term "functional" as used herein means that the structure and function of the human body have a beneficial effect on health uses such as controlling nutrients or physiological actions. The health functional food of the present invention can be prepared by a method commonly used in the art and can be prepared by adding raw materials and ingredients that are conventionally added in the art. In addition, the formulations of the above health functional foods may also be manufactured without limitations as long as they are acceptable as health functional foods. The food composition of the present invention can be manufactured in various formulations, and unlike general pharmaceuticals, it has an advantage of being free from side effects that may occur when a food is used as a raw material for a long period of time, and is excellent in portability. Health functional foods can be ingested as adjuvants to improve skin whitening, skin elasticity or wrinkle improvement, or skin moisturizing, antioxidant, anti-inflammatory effects.

The form of the health functional food of the present invention is not limited, and may include all foods in the conventional meaning, and may be used in combination with terms known in the art such as functional foods. In addition, the health functional food of the present invention can be prepared by mixing other suitable auxiliary ingredients, which may be included in food, according to the selection of a person skilled in the art, and known additives. Examples of foods that can be added include dairy products, such as meat, sausage, bread, chocolates, candies, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Vitamin complex, and the like, and can be prepared by adding to juice, tea, jelly, juice and the like prepared by using the compound represented by the formula (1) according to the present invention as a main component. It also includes foods used as feed for animals.

According to another aspect of the present invention, there is provided a quasi-drug composition comprising, as an active ingredient, a mixed extract of lotus flower, simvidium, and ganoderma.

In the present invention, the quasi-drug composition may include saccharides. Examples of the saccharides include honey, royal jelly, sugar, fructose, sugar alcohols, fructooligosaccharides, and the like, but not limited thereto, and any substance conventionally used in the art can be used.

In the present invention, the quasi-drug composition may have effects such as skin whitening, skin elasticity enhancement and wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory effects, but is not limited thereto.

The lotion, simvidium, algae, extract, mixed extract, honey, royal jelly, skin whitening, skin elasticity enhancement and wrinkle improvement, skin moisturizing, antioxidant, antiinflammation are as described above.

The quasi-drug composition of the present invention may further contain a pharmaceutically acceptable carrier, excipient or diluent as necessary in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, .

Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, acacia rubber, alginate, calcium phosphate, calcium Methyl cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oils such as sodium carboxymethylcellulose, , Injectable ester, witepsol, macrogol, tween 61, cacao paper, and laurie paper.

In addition, when the composition comprising the mixed extract of lotus flower, simvidium and gilt blend of the present invention as an active ingredient is used as a quasi-drug, it may further contain one or more active ingredients exhibiting the same or similar functions. For example, it may contain known skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing ingredients. The addition of skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing ingredients may further enhance skin whitening, elasticity enhancement, wrinkle enhancement and moisturizing effect of the composition of the present invention. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. The quasi-drug composition may be a whitening ingredient known in the art, such as a substance inhibiting tyrosinase enzyme activity such as kojic acid, arbutin, hydroquinone, L-ascorbic acid; Retinoic acid, TGF, proteins from animal placenta, betulinic acid and chlorella extract, as skin elasticity, wrinkle or moisturizing ingredients known in the art; And derivatives thereof, and various plant extracts. ≪ Desc / Clms Page number 2 > The additional component may be included in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety, ease of use and the like.

The quasi-drug composition of the present invention can be exemplified by a disinfectant cleaner, a shower foam, a softener solution, a wet tissue, a coating agent, and the like. The formulation method, dosage, usage method, And the like.

In addition, the quasi-drug composition comprising the lotus flower, the simvidium, and the ginseng blend extract of the present invention as an active ingredient may be used for skin whitening, skin elasticity enhancement or wrinkle improvement, Can be used for moisturizing methods. The subject includes, without limitation, mammals including rats, livestock, humans, and the like.

The cosmetic composition containing the lotus flower, the simvidium, and the mixture of ginseng as an active ingredient of the present invention is excellent in skin whitening, improving elasticity or wrinkle, skin moisturizing, antioxidant and anti-inflammatory effect. Specifically, the composition containing the mixed extract of the present invention exhibits an effect of reducing the total amount of melanin, promoting collagen synthesis and moisturizing effect, and exhibiting an NO production inhibitory effect and DPPH radical scavenging ability superior to a single extract, For use as a cosmetic composition for skin or wrinkle improvement, for skin moisturizing, for antioxidation and for antiinflammation. In addition, the composition containing the mixed extract of lotus flower, simvidium and yuzen as the active ingredient of the present invention can be used as a functional food composition and a quasi-drug composition.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Manufacturing example  1. Lotus flower, Symbium , A goldsmith  Preparation of extract

After washing with lotion, simvidium, and gyeolryeon with water, the washed lotus, symbium, and gyeolryeong were put into an extraction vessel, and an extraction solvent was added and slowly extracted at room temperature. The obtained extract was filtered to remove solid matter using filter paper, and the extract was concentrated under reduced pressure to prepare respective extracts.

Manufacturing example  2. Preparation of mixed extract

Simultaneous extracts of each of the lotus flower, simvidium, and gold leaf blend prepared in Preparation Example 1 were injected in the same amount to prepare a mixed extract. Mineral water or sugars such as honey and / or royal jelly were added to each mixed extract. Examples of the production ratio of the mixed extract are shown in Table 1.

ingredient Example  One( % ) Example 2  ( % ) Example 3  ( % ) Example 4  ( % ) Mineral water 25 - - - Lotus 25 25 25 25 Symbium 25 25 25 25 A goldsmith 25 25 25 25 honey - 25 - 12.5 Royal jelly - - 25 12.5

The contents of Examples 1 to 4 were determined on the basis of the respective extracts of Preparation Example 1. In the case of Examples 1 to 4, the maximum content ratios were adjusted so that the extracts were mixed at an equal ratio so that the synergistic effect could be obtained by the collaboration operation.

Experimental Example  1. Enhancement of collagen synthesis

The following extracts were prepared from the single extracts of each of the lotus flower, symbium, and gold leaf blades prepared in Preparation Example 1, the honey, royal jelly, and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2 to prepare collagen synthesis The enhancement effect was measured.

The synthesis of procollagen was measured using a kit (procollagen type-1 C-peptide EIA kit, Takara, Japan). HF (Human Fibroblast) cells were placed in a 24-well plate at a density of 5 x 10 ⁴ cells / ml, and each of the single extracts prepared in Production Example 1, no-added (control), honey, royal jelly, 0.01% by weight of the mixed extract of Examples 1 to 4 was added and cultured at 37 ° C in a 5% CO ₂ incubator for 48 hours. 100 μl of antibody-POD conjugate solution (antibody-POD conjugate sol ') in 96-well plate coated with anti-PIP antibody was added and added to each single 20 μl of the standard solution was added to the extract, honey, royal jelly and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, shaded with aluminum foil, and cultured at 37 ° C for 3 hours.

After removing the culture medium, the cells were washed 4 times with PBS, 100 μl of the substrate solution was added, and the mixture was allowed to stand at room temperature for 15 minutes. Then, 100 μl of H ₂ SO ₄ was added and shaken gently to measure absorbance at 450 nm. The experimental results are shown in Table 2. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Collagen expression Increase (%)    (n = 3) division Increase in collagen synthesis (%) No treatment 0 Lotus 10.08 Symbium 1.4 A goldsmith 12.5 honey 7.05 Royal jelly 11.73 Example 1 18.6 Example 2 22.6 Example 3 24.9 Example 4 27.35

As a result, as shown in Table 2, it was confirmed that when the mixed extract of Example 1 prepared in Preparation Example 2 was superior to each single extract prepared in Preparation Example 1, the collagen synthesis was increased, The extracts of Examples 2 to 4 produced more excellent collagen synthesis than Honey and Royal Jelly.

Experimental Example  2. Melanin formation inhibitory effect

In order to confirm the whitening effect of the single extract of each of the lotus flower, symbium, and gold leaf blades prepared in Production Example 1, honey, royal jelly and the mixed extracts of Examples 1 to 4 prepared in Production Example 2, The melanin inhibitory effect was measured using the cells.

Specifically, the whitening effect of the sample was evaluated by measuring the degree of reduction of the amount of melanin pigment present in the melanocyte. The sample was diluted to 0.01 wt%, treated with B16 melanocyte, cultured for 48 hours, and melanin was quantified to evaluate whitening efficacy. The melanin content was calculated as the ratio of the total protein to the DMSO control. The positive control group was Arbutin 100 ppm (Lim YJ et al., 2009, Arch Pharm Res.).

The results are shown in Table 3 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Melanin production (%)                      (n = 3) sample Melanin production (%) No treatment 100 Arbutin (positive control) 55 Lotus 68 Symbium 93 A goldsmith 75 honey 120 Royal jelly 146 Example 1 59 Example 2 48 Example 3 52 Example 4 40

As a result, as shown in Table 3, it was confirmed that melanin formation inhibitory effect was superior to each single extract prepared in Preparation Example 1 in the mixed extract treatment of Example 1 prepared in Preparation Example 2, The melanin production inhibitory effect of the mixed extracts of Examples 2 to 4 was better than that of the mixed extract of Example 1 by using honey and / or royal jelly.

Experimental Example  3. Moisturizing factor enhancing effect

In order to confirm the effect of enhancing the moisturizing factor synthesis factors on the single extracts of each of the lotus flower, symbium, and gold leaf flower prepared in Preparation Example 1, honey, royal jelly and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, The skin moisturizing effect was measured using the cultured cells as follows.

Specifically, at 37 ℃, conditions of _{5% CO 2 10% FBS (} Gibco), streptomycin (streptomycin) 100 ㎍ / ㎖, penicillin (penicillin) 100 ㎍ / ㎖, amphotericin B (amphotericin B) 0.25 ㎍ / (HaCaT, Human keratinocyte) were proliferated using DMEM medium supplemented with 10% fetal bovine serum.

The formed keratinocyte (HaCaT, Human keratinocyte) was dispensed into 6 wells at a density of 5 × 10 ⁵ cells / well with 3 ml of medium, and then cultured in a 5% CO ₂ incubator at 37 ° C. for 24 hours. The cultured medium was washed with PBS to remove FBS. To the medium on which the FBS was removed, 0.01% by weight of the single extract of each of the rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly and Examples 1, 2, 3 and 4 prepared in Preparation Example 2 After 3 ml each, the cells were cultured again and the amount of HAS3 (Hyaluronan Synthases 3) mRNA was compared. The cDNA was synthesized using SuperScript II reverse transcriptase (Invitrogen, USA), and the PCR was performed using a Platinum PCR supermix (Invitrogen, USA). The RNA was extracted using an RNA extraction kit (RNeasy Mini Kit, Qiagen, USA) (Invitrogen, USA)) and the prepared primers. The cDNA synthesis was performed by heating 1 μg of total RNA (total RNA) at 50 ° C. for 1 hour and at 85 ° C. for 5 minutes, and then the reaction was stopped. The PCR amplification was denatured at 94 DEG C for 50 seconds, annealed at 60 DEG C for 50 seconds, and then extended at 72 DEG C for 50 seconds. The amplification product was electrophoresed on 2% agarose gel and the gene expression was analyzed by Fusion Fx5 detection system (VilberLourmat, Germany). Respectively.

The results are shown in Table 4 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Increased expression of HAS (%)                 (n = 3) division HAS mRNA  Expression % ) No treatment 100 Lotus 119 Symbium 108 A goldsmith 113 honey 112 Royal jelly 115 Example 1 125 Example 2 136 Example 3 141 Example 4 147

As a result, as shown in Table 4, it was confirmed that the HAS expression was increased in the mixed extract treatment of Example 1 prepared in Preparation Example 2, compared with each single extract prepared in Preparation Example 1, It was confirmed that the HAS expression of the mixed extracts of Examples 2 to 4 was more enhanced by the honey and / or royal jelly than the mixed extract of Example 1.

Experimental Example  4. Antioxidant effect

In order to confirm the antioxidative effect of the single extract of each of lotus flower, symbium, and gold leaf blades prepared in Preparation Example 1, honey, royal jelly and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, And the skin antioxidant effect was measured.

Specifically, the radical scavenging ability of the sample was evaluated using DPPH changing from purple to yellow when radicals present in DPPH were erased. Vit C was used as a control. After dissolving the sample in methanol, 100 μl of 0.15 mM DPPH solution and 100 μl of sample were mixed and reacted at room temperature for 30 minutes. Then, the color change of DPPH was measured at 540 nm for absorbance (A540). Obtain the IC ₅₀ values were compared to a radical scavenging activity by the A540 value.

The results are shown in Table 5 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Radical Scatters  (IC ₅₀ ) (n = 3) division IC ₅₀ Vit C 0.0005 Lotus 0.53 Symbium 0.69 A goldsmith 0.64 honey 12.04 Royal jelly 6.049 Example 1 0.48 Example 2 0.42 Example 3 0.33 Example 4 0.2740

As a result, as shown in Table 5, it was confirmed that when the mixed extract of Example 1 prepared in Preparation Example 2 had an antioxidative effect superior to that of each single extract prepared in Preparation Example 1, It was confirmed that the mixed extracts of Examples 2 to 4 had better antioxidative effects than the mixed extract of Example 1 by using honey and / or royal jelly.

Experimental Example  5. Anti-inflammatory effect

To confirm the anti-inflammatory effect of the single extracts of each of the lotus flower, symbium, and gold leaf prepared in Preparation Example 1, honey, royal jelly and the mixed extracts of Examples 1 to 4 prepared in Preparation Example 2, The anti-inflammatory effect of the cells was measured.

Specifically, Raw 264.7 cells were used to measure the NO production inhibitory effect of the extracts. As a positive control, 20 mg / ml of L-NMMA1 (NOS inhibitor) was used. The sample was diluted to a concentration of 0.01% by weight and pretreated 30 minutes before treatment with 1 μg / ml LPS and cultured for 24 hours. Was used to evaluate the ability to inhibit NO production as follows.

The results are shown in Table 6 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

NO generation inhibitory effect (%)                  (n = 3) division NO production inhibitory effect ( % ) Positive control (NOS inhibitor) 42 Lotus 38 Symbium 25.6 A goldsmith 14.6 honey 13.1 Royal jelly 15.5 Example 1 39 Example 2 41.2 Example 3 42.8 Example 4 44

As a result, as shown in Table 6, the ability to inhibit NO production was confirmed to be higher than that of each single extract prepared in Preparation Example 1 in the mixed extract treatment of Example 1 prepared in Preparation Example 2, The mixed extracts of Examples 2 to 4 showed higher NO production inhibitory potency than the mixed extract of Example 1 by honey and / or royal jelly.

In other words, it can be seen that the mixed extract according to the present invention (Example 1) exerts superior synergistic action to each lotus flower, simvidium, and gold-smelt single extract, and through Examples 2 to 4, honey and / or royal jelly When added, the synergistic effect of the mixed extract is higher.

These results indicate that the mixed extract is very effective for skin whitening, skin elasticity enhancement or wrinkle improvement, skin moisturizing, skin aging prevention, antioxidation, and skin inflammation improving effect.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (13)

A cosmetic composition comprising, as an active ingredient, a mixed extract of lotus flower, simvidium, and rhododendron.
The method according to claim 1,
Wherein the cosmetic composition further comprises honey, royal jelly, or both.
3. The method according to claim 1 or 2,
Wherein the cosmetic composition is for skin whitening.
3. The method according to claim 1 or 2,
Wherein the cosmetic composition is for enhancing skin elasticity or improving wrinkles.
3. The method according to claim 1 or 2,
Wherein the cosmetic composition is for skin moisturizing.
3. The method according to claim 1 or 2,
Wherein the cosmetic composition is for antioxidation.
3. The method according to claim 1 or 2,
Wherein the cosmetic composition is anti-inflammatory.
A functional food composition comprising, as an active ingredient, a mixed extract of lotus flower, simvidium, and ganoderma.
9. The method of claim 8,
Wherein the composition further comprises honey, royal jelly, or both.
10. The method according to claim 8 or 9,
Wherein said functional food composition is for skin whitening, skin elasticity enhancement or wrinkle improvement, skin moisturizing, antioxidative or anti-inflammatory.
A quasi-drug composition comprising a mixed extract of lotus flower, simvidium and rhododendron as an active ingredient.
12. The method of claim 11,
A quasi-composition further comprising honey, royal jelly, or both.
13. The method according to claim 11 or 12,
The quasi-drug composition is a quasi-drug composition for skin whitening, skin elasticity enhancement or wrinkle improvement, skin moisturizing, antioxidant or anti-inflammatory.