KR102611505B1 - Composition for improving skin condition comprising herb extracts mixture - Google Patents

Abstract

The present invention relates to a composition containing as an active ingredient a complex herbal extract that is safe when applied to the skin and has excellent skin improvement effects. More specifically, the composition according to the present invention is a composition containing peach blossom extract, apricot extract, sweet confectionery extract, cypress extract, root extract, honey extract, and milk extract as active ingredients, and has anti-glycation effect, skin whitening effect, and skin elasticity. And it has excellent effects on skin improvement, including wrinkle improvement effect, antioxidant effect, and skin trouble improvement effect.

Description

Composition for improving skin condition comprising herb extracts mixture}

The present invention relates to a composition containing as an active ingredient a complex herbal extract that is safe when applied to the skin and has excellent skin improvement effects.

The skin is an important organ that protects the human body from the external environment and is responsible for a variety of physiological functions, such as a barrier function that prevents internal moisture and useful ingredients from leaking out, a temperature regulation function, and an excretion function. However, the activity of skin cells may decrease and the skin condition may worsen due to the following reasons.

Collagen and elastin present in the dermal layer of the skin have a great influence on the composition of the skin by performing functions such as mechanical robustness of the skin, resistance of connective tissue, maintenance of tissue cohesion, and support of cell adhesion. Collagen is reduced due to stress, aging, or photoaging due to UV irradiation, while elastin is known to have its three-dimensional structure distorted by the decomposing enzyme elastase, whose activity increases after exposure to UV rays. As a result, skin tissue becomes loose and loses elasticity.

As the cell function of the epidermis deteriorates, metabolism becomes less smooth, and exfoliation does not occur easily, so when keratin is exposed to ultraviolet rays while excessive accumulation of keratin occurs, elasticity decreases and wrinkles occur. Alternatively, if a sebum film is not formed on the epidermis, moisture and sebum secretion decreases, the skin dries out, and wrinkles form.

When the skin receives ultraviolet rays, it synthesizes melanin to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through melanosomes. These keratinocytes turn over every 28 days. Therefore, the produced melanin is generally lost by keratinocytes every 28 days, but if the skin cell cycle is not properly regulated due to stress, skin aging, etc., keratinocytes do not fall off at the right time, causing pigmentation such as spots, freckles, and age spots. Calmness occurs.

On the skin, sebum, sweat, and cosmetic ingredients are decomposed into highly toxic substances by skin flora, which can irritate the skin and cause inflammation. Additionally, irritation from ultraviolet rays can increase the production of nitric oxide (NO), an inflammatory mediator, in the skin, causing skin problems. The production of nitric oxide is mostly caused by iNOS, and iNOS is known to be rapidly induced by stimuli such as LPS and cytokines, producing excessive amounts of NO.

Reactive oxygen species (ROS), also known as toxic oxygen species, are toxic substances generated in cells through physiological processes such as respiration and are constantly produced and destroyed. Under normal conditions, they exist at around 3-5%. . These reactive oxygen species are free radicals such as superoxide radical (O2-) and hydroxyl radical (HO+): Chemically, they are atoms or molecules that are not paired in the outermost electron orbit. It exists in a highly unstable and highly reactive form) or in the form of a compound with paired electrons, such as hydrogen peroxide (H ₂ O ₂ ) or singlet oxygen (singlet radical). Reactive oxygen species have the advantage of having a biological defense function that sterilizes bacteria within the physiological system, but they generally have a harmful effect that causes disease by causing oxidation in the living body. There are reports that these reactive oxygen species attack biological molecules, causing damage to cells and tissues, and causing various types of diseases related to aging and various adult diseases.

Research has continued to develop substances that suppress skin aging caused by the various factors mentioned above, improve whitening, wrinkles, elasticity or skin trouble, and have antioxidant effects.

Substances with the above-mentioned effects are widely distributed in the natural world, and mainly substances derived from plants have been used as raw materials for foods, cosmetics, and medicines. However, these substances derived from the natural world are not very effective and must be used in large quantities to obtain significant effects, which has led to problems of toxicity and increased prices.

To solve these problems with natural materials, the development of chemically synthesized materials continued. These have the advantage of showing far superior effects compared to natural substances even when used in small amounts, but on the other hand, they have the fatal problem of causing large and small side effects in the human body, which limits their use.

Accordingly, there is an urgent need for the development of substances that are derived from natural products and ensure stability while demonstrating excellent skin improvement effects.

The problem to be solved by the present invention is to provide a complex herbal extract that is derived from natural products and ensures stability while exhibiting excellent effects on skin improvement, especially whitening, wrinkle, elasticity, trouble improvement, and anti-oxidation.

In order to solve the above-mentioned problems, the present invention provides a skin whitening, wrinkle, elasticity, trouble, or anti-oxidant agent comprising peach blossom extract, apricot extract, sweet confectionery extract, cypress extract, root extract, honey extract, and milk extract as active ingredients. A composition (e.g., a cosmetic composition) is provided.

[extract]

Peach blossoms are the flowers of the peach tree Prunus persica (L.) Batsch. The taste is bitter and the properties are flat. It acts on the stomach meridian, liver meridian, and nerves.

Armeniacae Semen (杏仁) is the apricot tree Prunus armeniaca Linne var. ansu Maximowicz, Prunus mandshurica Koehne var. glabra Nakai, is the ripe seed of Siberian apricot Prunus sibirica Linne or Armenian apricot Prunus armeniaca Linne (Rosaceae). It removes phlegm, stops coughing and asthma, and moisturizes the intestines.

The sweetener is the seed of the melon Cucumis melo . The taste is sweet and the nature is cold. It removes stagnant blood, disperses clumps, removes heat from the lungs, quenches thirst, and promotes bowel movement.

Achyranthes Root is the root of Achyranthes japonica Nakai or Achyranthes bidentata Blume (Amaranthaceae). The appearance is in the form of a thin and long cylindrical main root or a main root with lateral roots, and the root head has a few rhizomes attached or removed. The main roots are usually rod-shaped or slightly curved, and the outer surface is gray-yellow or yellow-brown, with many vertical wrinkles and sparse marks of lateral roots. Pharmacological effects such as uterine stimulation, cholesterol-lowering, diuretic, blood sugar-lowering, and liver function improvement have been reported. It has almost no odor and is mucus-like. The taste is slightly bitter and sour, and the nature is flat without being biased towards one side.

Polygala Root is the root of Polygala tenuifolia Willdenow (Polygalaceae). The roots are thick and long, with several main stems coming out in a bunch at the end, and are almost hairless. The leaves are alternate, line-shaped, 1.5 to 3 cm long, and have no petioles. Flowers bloom in purple from July to August and grow sparsely on racemes. There are 5 calyx pieces, the 2 at the back and bottom are line-shaped, and the 2 on either side look like petals and are membranous (translucent like thin paper). The upper part of the petal is open, the lower part is attached, and the end is finely split like a brush. The fruit is a capsule that is flat and splits into two, and the seeds are densely hairy. In Oriental medicine, the root is called Wonji and is used as an expectorant, tonic, and tonic. Distributed north of central Korea and northern China.

Honey (蜜) is a sugary secretion collected and stored by honey bees (Apis mellifera). Honey includes bee honey (natural honey) and molasses (artificial honey). Honey is divided into native honey and beekeeping honey depending on the type of bee. Depending on the flower, it is called acacia honey, bush honey, rape honey, chestnut honey, buckwheat honey, etc., and the color and taste vary depending on the type of flower.

Cow's milk is a white, opaque liquid with a unique flavor and sweetness secreted from the mammary glands of cows.

The present inventors have found that all of the above extracts have biological activity beneficial to the skin, and a composite extract of two or more of the above extracts, preferably a composite extract of peach blossom extract, apricot extract, sweet confectionery extract, sycamore extract, root extract, honey extract and milk extract. was found to have synergistic biological activity.

In the present invention, the term "extract" refers to the extract itself, such as the extract obtained by the extraction treatment, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a crude product or purified product of the extract, or a mixture thereof. and extracts of all formulations that can be formed using the extract.

The extract describes each plant and may include, in addition to the specified parts, its leaves, stems, bark, roots, flowers or buds, fruits, seeds, sap, and the entire plant.

To prepare the extract, a person skilled in the art may use any suitable method known in the art. For example, it may be by solvent extraction. The whole plant or any part may be ground (e.g., in a blender) and then treated with an extraction solvent to obtain a solvent extract. Before pulverizing, it may be subjected to a drying process (for example, drying at 40 to 70° C. for 15 to 50 hours) and then pulverized. Additionally, the solvent extract can be prepared in powder form by additional processes such as reduced pressure distillation and freeze-drying or spray drying.

The type of extraction solvent used is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Alternatively, a mixture thereof can be used. Preferably, water and lower alcohol can be used alone or in a mixture of two or more types. A solvent extract can be prepared by extracting the solvent more than once using the solvent, and a dried extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray-drying the extract can be prepared.

The amount of the extraction solvent may vary depending on the type of extraction solvent used, but for example, it can be used in an amount of 1 to 20 times, or 5 to 20 times, the dry weight of the target plant.

In addition, various extraction processes known in the art, such as maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous-alcoholic extraction , countercurrent extraction, microwave-assisted extraction, ultrasonic extraction, supercritical fluid extraction, phytonic extraction (e.g., hydro-fluoro-carbon solvent), etc.) can be selected and used, and they can be performed alone or It can be performed by using two or more methods in combination.

[Composition]

The composition according to the present invention contains peach blossom extract, apricot extract, sweet confectionery extract, cypress extract, root extract, honey extract and milk extract as active ingredients.

In the present invention, “included as an active ingredient” means that the extract is added to the extent that it can exhibit a skin improvement effect from the composition according to the present invention, and various ingredients are added as sub-ingredients for skin delivery and stabilization. This means that it can be formulated in various forms.

Each plant extract may be included in the composition according to the present invention in the following ratio. For example, the weight ratio of peach blossom extract, apricot extract, apricot extract, sycamore extract, root extract, honey extract and milk extract is 1: 0.01 ~ 10: 0.01 ~ 10: 0.01 ~ 10: 0.01 ~ 10: 0.01 ~ 10: 0.01 ~ 10 or 1: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10, 1: 1 ~ 5: 1 ~ 5: 1 ~ 5: 1 ~ 5: 1 ~ It may be included as 5: 1 to 5, or 1: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3.

The composition according to the present invention may contain at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012%, 0.0013%, 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.00 24%, 0.0025%, 0.0026%, 0.0027% , 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0. 0041%, 0.0042%, 0.0043%, 0.0044 %, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0 .0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%, 0.0063%, 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.00 74%, 0.0075%, 0.0076%, 0.0077% , 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0. 0091%, 0.0092%, 0.0093%, 0.0094 %, 0.0095%, 0.0096%, 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0 .0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.08 00%, 0.0825%, 0.0850%, 0.0875% , 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000%, 0.2250%, 0.2500%, 0.2750%, 0.3000%, 0. 3250%, 0.3500%, 0.3750%, 0.4000 %, 0.4250%, 0.4500%, 0.4750%, 0.5000%, 0.5250%, 0.550%, 0.5750%, 0.6000%, 0.6250%, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0. 7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.7%, 1.7%, 1.8%, 1.9% , 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6 %, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9% , 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9%, 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6 %, 8.7%, 8.8%, 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% , 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99% or more of one or more of the present invention. May contain extracts.

In addition, the composition according to the present invention contains at least about 0.0001% to 90%, 0.001% to 90%, 0.01% to 90%, 0.1% to 90%, 0.1% to 90% of each extract of the present invention in the final form of the composition. %, 0.1% to 80%, 0.1% to 70%, 0.1% to 60%, 0.1% to 50%, 0.1% to 40%, 0.1% to 30%, 0.1% to 20% or 0.1% to 10%. may be included in the scope.

The % can be calculated based on weight relative to the total weight of the composition or volume relative to the total volume, and the concentration may be adjusted depending on the desired effect of the composition or the product into which the composition is incorporated.

The compositions of the present invention can be formulated into all types of vehicles. Examples of suitable vehicles include emulsions (e.g., oil-in-water, water-in-oil, silicone-in-water, water-in-silicone, water-in-oil-in-water, oil-in-water, oil-in-water-in-oil, oil-in-silicone, etc.), creams, lotions, solutions (e.g., aqueous and hydro-alcoholic solutions), anhydrous bases (e.g., lipsticks and powders), gels, ointments, pastes, milks, liquids, aerosols, solid forms, or eye jellies.

Additionally, the compositions of the present invention may be encapsulated for delivery to a target area, such as the skin. Encapsulation techniques include, for example, liposomes, vesicles, and/or nanoparticles that can be used as delivery vehicles to deliver ingredients to the skin (e.g., polymeric materials in which ingredients are trapped, encapsulated, and/or absorbed). It may include, but is not limited to, the use of biodegradable and non-biodegradable colloidal particles, including, for example, nanospheres and nanocapsules.

The composition according to the present invention can be commercialized in various ways. For example, it can be commercialized into cosmetic compositions, food compositions, etc.

For example, the cosmetic composition may be prepared in the form of a general emulsified formulation or solubilized formulation. For example, lotion such as flexible lotion or nourishing lotion, emulsion such as facial lotion, body lotion, cream such as nutritional cream, moisture cream, eye cream, massage cream, essence, serum, cosmetic ointment, spray, oil gel, gel , packs, sunscreens, makeup bases, foundations such as liquid type, solid type or spray type, makeup removers such as powder, cleansing cream, cleansing lotion, cleansing oil, cleansing agents such as cleansing foam, soap, body wash, etc. However, it is not limited thereto and may be formulated in various forms known in the art.

The cosmetic composition may optionally further include, in addition to the extract according to the present invention, conventional components known as components of cosmetic compositions in the art, as long as they do not impair the purpose of the present invention. For example, fragrances (synthetic and natural), dyes and color ingredients, absorbents, emulsifiers, stabilizers, lubricants, solvents, humectants (e.g. emollients, humectants, film formers, occlusives). agents, and agents that affect the skin's natural moisturizing mechanisms), water-repellants, UV absorbers (physical and chemical absorbers, such as para-aminobenzoic acid ("PABA") and PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins (e.g. A, B, C, D, E and K), trace metals (e.g. zinc, calcium and selenium), anti- Anti-irritants (e.g. steroids and non-steroidal anti-inflammatory drugs), anti-microbial agents, antioxidants (e.g. BHT and tocopherol), chelating agents (e.g. disodium EDTA and tetrasodium EDTA) , preservatives (e.g. methylparaben and propylparaben), pH adjusters (e.g. sodium hydroxide and citric acid), absorbents (e.g. aluminum starch octenylsuccinate, kaolin, corn starch, oat starch, cyclodextrins, talc). ), and zeolites), skin bleaching and lightening agents (e.g. hydroquinone and niacinamide lactate), humectants (e.g. glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol) , urea and mannitol), exfoliants (e.g. alpha-hydroxy acids and beta-hydroxy acids such as lactic acid, glycolic acid and salicylic acid; and their salts), waterproofing agents (e.g. magnesium/aluminum hydroxide stearate), skin conditioning agents (e.g. aloe extract, allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium) glycyrrhizate), and thickening agents (e.g. substances capable of increasing the viscosity of the composition, such as carboxylic acid polymers, cross-linked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums) and silicon-containing compounds (e.g., silicone oil and polyorganosiloxane).

The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc.). It is not limited to this. The supplements may include vitamins, minerals, herbs or other plants, amino acids, enzymes, and metabolites. These adjuvants are suitable for oral consumption and can be administered orally.

It may be provided as a kit containing the composition according to the present invention. The composition according to the invention is contained in a container, which can contain a bottle, metal tube, laminated tube, plastic tube, dispenser, pressure vessel, barrier container, package, compartment, lipstick container, compact container, cosmetic composition. This may include, but is not limited to, a cosmetic pan or other type of container, such as a plastic container injected or blow-molded into a desired bottle, dispenser, or package in which the dispersions or compositions are held. . The kit may include instructions for using the kit or composition, which may be written on a separate sheet of paper, or may be written on the surface of the container, the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures, or symbols. Instructions may include, for example, instructions on how to use, apply, and maintain the kit or composition. The container can be distributed and contained in a predetermined amount.

Compositions according to the invention may be provided as topical skin compositions.

Additionally, a method of topically applying the composition according to the present invention to the skin can be provided.

In the present invention, the term “topical application” means applying or spreading a composition onto the surface of keratinous tissue, and “topical skin composition” includes compositions suitable for topical application or spreading onto keratinous tissue. These compositions are typically dermatologically acceptable in that they do not have excessive toxicity, incompatibility, instability, allergic reactions, etc. when applied to the skin. The topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.

[Skin improvement effect]

The composition according to the present invention has biological activity and exhibits excellent skin improvement effects.

More specifically, the composition according to the present invention has a skin whitening effect.

The skin whitening refers to suppressing melanin production by suppressing the melanin formation process.

As a result of measuring the amount of melanin produced by treating melanoma cells with the complex extract according to the present invention, the present inventors were able to confirm that an increased melanin production inhibition effect was observed compared to the case of treating each extract, and skin whitening It was found that the effect was equivalent to that of arbutin, which has known effects. It was confirmed that the composition according to the present invention has an excellent melanin production inhibition effect and exhibits an excellent whitening effect.

Additionally, the composition according to the present invention has the effect of improving wrinkles and elasticity.

The skin wrinkles refer to fine lines that appear as the skin ages, and can be caused by genes, a decrease in collagen and elastin present in the skin dermis, and external environments.

The wrinkle improvement refers to suppressing or inhibiting the formation of wrinkles, or alleviating wrinkles that have already been formed.

The skin elasticity is caused by elastic fibers composed of elastin and collagen fibers called collagen present in the dermal layer. The elasticity improvement refers to suppressing or inhibiting the decrease in elasticity, or maintaining or improving elasticity. .

As a result of processing the complex extract according to the present invention, the present inventors were able to confirm that the effect of promoting collagen synthesis and inhibiting elastase activity was increased compared to the case of processing each extract, and the effect of promoting skin collagen synthesis was known to be effective. It was found to have an effect comparable to that of vitamin C, which is known to have an effect equivalent to that of quercetin, which is known to inhibit skin elastase activity. It was confirmed that the composition according to the present invention has an excellent effect of promoting collagen synthesis and inhibiting elastase activity, and exhibits an excellent effect of improving skin wrinkles and elasticity.

Additionally, the composition according to the present invention has a trouble improving effect.

The above trouble refers to symptoms such as skin irritation and inflammation, and the inflammatory response is characterized by pain, fever, redness, swelling, and loss of function due to external stimuli. Histologically, it is characterized by permeability and blood flow of arterioles, capillaries, and venules. Complex symptoms, including expansion accompanied by increase, exudation of plasma containing plasma proteins, and migration of white blood cells to the inflamed area, are observed, and skin irritation reactions are characterized by erythema, itching, and fever.

The trouble improvement refers to suppressing or inhibiting skin irritation and inflammatory reactions, or relieving skin irritation or inflammation reactions that are already in progress.

As a result of measuring the NO production inhibition effect by treating the complex extract according to the present invention, the present inventors were able to confirm that the NO production inhibition effect was increased compared to the case of treating each extract, and the NO production inhibition effect is known. It was found that it exerts an effect equivalent to that of L-NMMA. It was confirmed that the composition according to the present invention exhibits an excellent skin trouble improvement effect by inhibiting NO production.

Additionally, the composition according to the present invention has an antioxidant effect.

The antioxidant refers to suppressing oxidation of cells by highly reactive free radicals or reactive oxygen species (ROS) due to oxidative stress caused by intracellular metabolism or the influence of ultraviolet rays. Alternatively, it includes removing reactive oxygen species and reducing damage to cells caused by them.

As a result of measuring the free radical scavenging ability by treating the complex extract according to the present invention, the present inventors confirmed that the free radical scavenging ability was increased compared to the case of treating each extract, and vitamin C, which is known to have free radical scavenging ability, It was found that it had a corresponding effect. As a result, it was confirmed that the composition according to the present invention exhibits excellent antioxidant effect.

Additionally, the composition according to the present invention has an anti-glycation effect.

The glycation refers to a phenomenon in which sugar and protein combine due to the presence of excessive sugar and the protein is destroyed. As glycation progresses, it affects collagen and elastin, causing various skin damage as well as decreased skin elasticity.

As a result of measuring the anti-glycation effect by treating the complex extract according to the present invention, the present inventors confirmed that the anti-glycation effect was increased compared to the case of treating each extract, and aminoguanidine, which is known to have an anti-glycation effect, It was found that it had a corresponding effect. As a result, it was confirmed that the composition according to the present invention exhibits excellent anti-glycation effect.

The complex extract according to the present invention is safe for the skin and has excellent skin improvement effects, including anti-glycation effect, skin whitening effect, skin elasticity and wrinkle improvement effect, antioxidant effect, and skin trouble improvement effect. Additionally, it has a fibroblast proliferation effect.

Hereinafter, the present invention will be described in detail using examples and experimental examples to specifically explain the present invention. However, the examples and experimental examples according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the examples and experimental examples described in detail below. Examples and experimental examples of the present invention are provided to more completely explain the present invention to those with average knowledge in the art.

<Example 1> Preparation of peach blossom extract

After drying the peach blossoms well and cutting them into small pieces, 100 g of the dry weight was placed in a flask and extracted by cold soaking with 1000 g of extraction solvent (distilled water) for 3 days. The cold-soaked extract was filtered through a filter with a pore size of 0.2㎛ to prepare a peach blossom extract.

<Example 2> Preparation of apricot extract

After thoroughly drying and cutting the apricots, 100 g of the dried weight was placed in a flask and extracted by cold soaking with 1000 g of extraction solvent (distilled water) for 3 days. The cold-soaked extract was filtered through a filter with a pore size of 0.2㎛ to prepare an apricot extract.

<Example 3> Preparation of sweetened confectionery extract

After drying the sweetened confectionery well and cutting it into small pieces, 100 g of the dry weight was placed in a flask and extracted by cold soaking with 1000 g of extraction solvent (distilled water) for 3 days. The cold-soaked extract was filtered through a filter with a pore size of 0.2㎛ to prepare a confectionery extract.

<Example 4> Preparation of Hyssop extract

After drying and cutting the lyssop well, 100 g of the dry weight was placed in a flask and extracted by cold soaking with 1000 g of extraction solvent (distilled water) for 3 days. The cold-soaked extract was filtered through a filter with a pore size of 0.2㎛ to prepare a Hyssop extract.

<Example 5> Preparation of root extract

After drying the base paper well and cutting it into small pieces, 100 g of the dry weight was placed in a flask and extracted by cold soaking with 1000 g of extraction solvent (distilled water) for 3 days. The cold-soaked extract was filtered through a filter with a pore size of 0.2㎛ to prepare a root extract.

<Example 6> Preparation of honey extract

100g of honey was placed in a flask and extracted by heating with 1000g of extraction solvent (distilled water) for 30 minutes. A honey extract was prepared by filtering the extract through a filter with a pore size of 0.2㎛.

<Example 7> Preparation of milk extract

100g of milk was placed in a flask and extracted with 1000g of extraction solvent (distilled water). Milk extract was prepared by filtering the extract through a filter with a pore size of 0.2㎛.

<Example 8> Preparation of mixed extracts of peach blossoms, apricots, confectionery, sagebrush, raw materials, honey, and milk

As in the above-mentioned examples, equal amounts of peach blossoms, apricots, sweet confectionery, apricot root, honey, and milk prepared in the form of extracts were injected to prepare a mixed extract of peach blossoms, apricots, sweet confectionery, apricot root, honey, and milk. .

<Experimental Example 1> Anti-glycation effect

To confirm the anti-glycation effect, the glycation inhibition activity was measured using L-arginine and glucose.

First, 1M L-arginine and 1M glucose were prepared by dissolving them in 1M phosphate buffer solution (pH 7.4), and the sample was diluted to 50ppm using 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer solution were mixed in a ratio of 1 to 4, and then 80 μl each was dispensed into a 96-well plate. Here, 100 μl of each sample diluted to 50 ppm and 0.01 M aminoguanidin to be used as a positive control were added. After mixing these samples well, glucose diluted with 1M phosphate buffer solution was added so that the final concentration of glucose was 0.1M, and then reacted at 70°C for 4 hours. The degree of glycosylation was measured by measuring the absorbance of the 96-well plate at 420 nm using a spectrophotometer.

The Glycation experimental group shown below is an experimental group in which 1M L-arginine and 1M glucose were added to induce glycation. To measure the absorbance of the sample itself, only 1M L-arginine and the sample were added without adding glucose, and the absorbance was measured at 420 nm. Glycosylation inhibition activity can be calculated using the following formula.

[Equation 1]

('Glycation experimental group' in Equation 1 above means 'absorbance of the glycation experimental group'.)

As shown in the results in Table 1, the mixed extract has a more excellent anti-glycation effect compared to aminoguanidin, a known anti-glycation substance.

<Experimental Example 2> Melanin production inhibition effect

In order to confirm the whitening effect through inhibition of melanin production, in the culture medium of B-16 mouse melanoma cells according to the method described by Lotan R. et al. (Cancer Res. 40:3345-3350, 1980), The total amount of melanin was measured by adding the extract. During the experiment, toxicity to rat melanoma cells was first evaluated and whitening evaluation was performed at a non-toxic concentration. DMSO was used as a negative control, and arbutin was used as a positive control.

Specifically, the sample was added to the medium to a final concentration of 100 ppm, arbutin was added to the medium to a final concentration of 100 ppm, and the melanoma cells were cultured for 3 days. Afterwards, the cells were treated with trypsin, removed from the culture vessel, centrifuged, and melanin was extracted. To the removed cells, 1 ml of sodium hydroxide solution (1N concentration) was added and boiled for 10 minutes to dissolve the melanin. The amount of melanin produced was measured by measuring the absorbance at 400 nm using a spectrophotometer.

The amount of melanin was measured by expressing the absorbance per ^unit cell (1 It was performed and expressed as an average value.

As can be seen from the results in Table 2 above, the mixed extract showed an excellent effect in reducing the total amount of melanin and was found to be useful for whitening purposes.

<Experimental Example 3> Anti-inflammatory effect

To confirm the anti-inflammatory effect and skin trouble improvement effect, a nitric oxide (NO) production inhibition test was conducted using the GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, which are mouse macrophages, were subcultured several times, placed in a 24-well plate at 3×10 ⁵ cells per well, and cultured for 24 hours. Subsequently, the cell medium was replaced with a cell medium containing the sample at a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), an NO-production inhibitor, was treated as a positive control and cultured for 30 minutes, and 1 μg of LPS (Lipopolysaccharide) was treated as a stimulant and cultured for 24 hours. Take 100 ㎕ of the supernatant and transfer it to a 96-well plate, add 100 ㎕ of GRIESS solution each, react at room temperature for 10 minutes, measure the absorbance at 540 nm to determine the NO inhibition effect, and the NO production inhibition rate (%) is as follows. It was calculated using Equation 2 and shown in Table 3 below. The experiment was performed three times each and expressed as the average value.

[Equation 2]

NO production inhibition rate (%) = {(absorbance of negative control group - absorbance of each extract)/absorbance of negative control group} x 100

As can be seen from the results in Table 3 above, when compared to L-NMMA, a representative anti-inflammatory medicinal substance, the mixed extract had a slightly lower relative activity, but showed excellent activity as a natural substance.

<Experimental Example 4> Effect of promoting collagen synthesis

The sample was added to the culture medium of human-derived fibroblasts to confirm the effect of promoting type 1 collagen synthesis at the cellular level. The synthesized collagen was quantified using the PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT). To measure the amount of collagen synthesis, the sample was added to the fibroblast culture medium (DMEM medium) to a final concentration of 10 ppm, cultured for 48 hours, and then the culture medium was taken and the degree of type 1 collagen synthesis was measured at each concentration using the PICP EIA kit. Measured at 450 nm using a spectrophotometer.

To compare the effects, the degree of collagen synthesis was measured using the same method for samples to which untreated fibroblast culture medium (negative control) and vitamin C (positive control) were added to a final concentration of 52.85 ㎍/ml. The rate of increase in collagen production was calculated as the ratio of collagen production relative to the negative control group, and the results are shown in Table 4 below. The experiment was performed four times and expressed as the average value.

As can be seen from the results in Table 4, treatment with the mixed extract increased collagen synthesis, and the effect on collagen synthesis was similar to that of applying vitamin C, which is generally known to induce collagen synthesis.

<Experimental Example 5> Elastase activity inhibition effect

The effect of inhibiting the activity of Elastase, an enzyme that decomposes elastin, was confirmed as follows.

Elastase derived from human white blood cells was used, and the synthetic substrate MeOSuc-Ala-Ala-Pro-Val-pNA was used as the elastase substrate. A 100mM Tris (pH 7.5) solution was used as the buffer solution. Elastase was used in a final amount of 0.2 mU using a buffer solution. In addition, the synthetic substrate of elastase was made into a 100mM solution using DMSO and then diluted using a buffer solution to reach a final concentration of 0.5mM. At this time, the positive control was set to include Quercetin, known as an elastase inhibitor, at a concentration of 10ppm. The elastase inhibition candidate was added to a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and reacted at room temperature for 20 minutes. Absorbance was measured at 405 nm at 1-minute intervals using a spectrophotometer, and the slope of absorbance versus time was determined to determine enzyme activity. The elastase inhibition rate was calculated as follows.

[Equation 3]

The elastase inhibition rate was calculated using Equation 3 above and shown in Table 5 below. The experiment was performed three times each and expressed as the average value.

As can be seen from the results in Table 5, when the mixed extract was treated, the effect was relatively weaker than that of the positive control group, but it was found to exhibit an excellent elastase activity inhibition effect. Therefore, it was found that the mixed extract can be used for skin regeneration and wrinkle improvement.

<Experimental Example 6> Antioxidant effect

The free radical scavenging ability of the mixed extract was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical. When it exists in a radical state, it shows maximum light absorption at 517 nm and loses light absorption when the radical is eliminated. DPPH was used from Sigma, and was dissolved in methyl alcohol at a concentration of 0.15mM.

First, 100 μl of the mixed extract or vitamin C, a positive control, was added to each well of a 96-well plate. 100 μl of DPPH solution was added here, left at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).

The results of the concentration of the extract expressed as IC ₅₀ when the absorbance of the treated sample is half that of the control are shown in Table 6 below. This experiment was repeated three times.

As shown in Table 6 above, the mixed extract had a very strong free radical scavenging ability even when compared to vitamin C, thereby confirming its excellent antioxidant effect.

Claims (4)

A cosmetic composition for skin whitening, wrinkles, elasticity, skin troubles, or anti-oxidation, containing peach extract, apricot extract, sweet confectionery extract, cypress extract, root extract, honey extract, and milk extract as active ingredients. The method of claim 1, wherein the weight ratio of the peach blossom extract, apricot extract, sweet confectionery extract, cypress extract, root extract, honey extract and milk extract is 1: 0.01 ~ 10: 0.01 ~ 10: 0.01 ~ 10: 0.01 ~ 10: 0.01 ~ 10: A cosmetic composition characterized in that 0.01 to 10. The cosmetic composition according to claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohol, or a mixture thereof. The cosmetic composition of claim 1, wherein the cosmetic composition has anti-glycation properties, inhibits melanin production, promotes collagen synthesis, inhibits elastase activity, or scavenges free radicals.