KR102561148B1 - Composition for improving skin condition comprising herb extracts mixture - Google Patents

Abstract

The present invention relates to a composition comprising, as an active ingredient, a composite herbal medicine extract that is safe when applied to the skin and has excellent skin improvement effects. More specifically, the composition according to the present invention is a composition containing angelica quasi extract, peony extract, cnidium extract, and sukjihwang extract as active ingredients, and has excellent effects on skin improvement, including antiglycation effect, skin whitening effect, and skin trouble improvement effect.

Description

Composition for improving skin condition comprising herb extracts mixture

The present invention relates to a composition comprising, as an active ingredient, a composite herbal medicine extract that is safe when applied to the skin and has excellent skin improvement effects.

The skin is an important organ that protects the human body from the external environment and is in charge of various physiological functions such as a barrier function that prevents internal moisture and useful components from leaking out, a body temperature control function, and an excretion function. However, the activity of skin cells is reduced and the skin condition is deteriorated due to the following reasons.

When the skin is exposed to UV rays, melanin is synthesized to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through melanosomes. These keratinocytes turn over every 28 days. Therefore, it is common for the generated melanin to be lost every 28 days by keratinocytes, but if the skin cell cycle is not well regulated due to stress, skin aging, etc., keratinocytes do not drop out in time, resulting in pigmentation such as spots, freckles, and age spots.

In the skin, sebum, sweat components, and cosmetic ingredients are decomposed into highly toxic substances by skin flora, which can cause skin irritation and inflammation. In addition, irritation caused by ultraviolet rays may cause skin trouble by increasing the production of nitric oxide (NO), an inflammatory mediator in the skin. Nitric oxide is mostly produced by iNOS, and iNOS is known to be rapidly induced by stimuli such as LPS and cytokines to produce excess NO.

Research has been conducted to develop a substance that suppresses skin aging caused by the various factors described above and improves whitening or trouble.

Substances having the above effects are widely distributed in the natural world, and have been mainly used as raw materials for foods, cosmetics, and pharmaceuticals using substances derived from plants. However, such materials derived from nature do not have significant effects and must be used in large amounts to obtain significant effects, resulting in toxicity and price increase problems.

In order to solve these problems of natural materials, chemically synthesized materials have been developed. These have the advantage of exerting a much superior effect even with a small amount compared to natural substances, but on the other hand, there is a fatal problem that can cause large and small side effects in the human body, so their use is limited.

Accordingly, there is an urgent need to develop a substance derived from a natural product and ensuring stability while exhibiting an excellent skin improvement effect.

KR 1020060092179 A KR 1020090002682 A

The problem to be solved by the present invention is to provide a composite herbal extract that is derived from a natural product and secures stability while exhibiting excellent effects on skin improvement, particularly whitening and trouble improvement.

In order to solve the above problems, the present invention provides a composition (for example, a cosmetic composition) for skin whitening or trouble improvement containing angelica extract, peony extract, cnidium extract, and sukjihwang extract as active ingredients.

[extract]

Angelica Gigas belongs to the Apiaceae family, contains the root of Angelica Gigas Nakai , and has the property of making the five organs healthy and warming the body.

Peony (芍藥) belongs to the peony family ( Paeoniaceae ) lactiflora Pallas) or other related plants of the same genus.

Cnidium belongs to the family Umbelliferae. officinale Makino) or Chinese Cnidium (中國川芎, Ligusticum) chuanxiong Hort) as it is or blanched in boiling water.

Sukjihwang (熟地黃) is steamed and dried roots of Rehmannia glutinosa (Gaertner) Liboschitz ex Steudel of the Scrophulariaceae family, and is known to be effective in darkening hair color and recovering from fatigue.

The present inventors have found that all of the above extracts have biological activities beneficial to the skin, and the complex extracts of two or more of the above extracts, preferably the complex extracts of Angelica gigas extract, Peony extract, Cnidium japonica extract, and Sukjihwang extract have synergistic biological activities.

In the present invention, the term "extract" includes an extract obtained by the extraction treatment, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, and the extract itself and extracts of all formulations that can be formed using the extract.

The extract describes each plant and may include its leaves, stems, barks, roots, flowers or flower buds, fruits, seeds, sap and whole plants in addition to the specified parts.

To prepare the extract, one skilled in the art can use any suitable method known in the art. For example, it may be by a solvent extraction method. A solvent extract can be obtained by crushing the whole plant or any part thereof (eg, in a blender) and then treating the extraction solvent. It may be pulverized after undergoing a drying process (for example, drying at 40 to 70° C. for 15 to 50 hours) before pulverization. In addition, the solvent extract can be prepared in a powder state by additional processes such as distillation under reduced pressure and lyophilization or spray drying.

The type of extraction solvent used is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, and preferably, water and lower alcohol may be used alone or in combination of two or more. A solvent extract may be prepared by extracting at least once using the solvent, and a dried extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray-drying may be prepared.

The amount of the extraction solvent may vary depending on the type of extraction solvent used, but may be used, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.

In addition, various extraction processes known in the art, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous-alcoholic extraction, countercurrent extraction, microwave assisted extraction, ultrasonic extraction, supercritical fluid extraction, phytonic extract (eg, hydro-fluoro-carbon solvent), etc.) may be selected and used, and these may be performed alone or Two or more methods may be used in combination.

[composition]

The composition according to the present invention includes Angelica Quail extract, Peony extract, Cnidium japonica extract, and Sukjihwang extract as active ingredients.

In the present invention, the meaning of "included as an active ingredient" means that an extract is added to the extent that can exhibit a skin improvement effect from the composition according to the present invention, and various ingredients are added as subcomponents for skin delivery and stabilization. It means that it can be formulated in various forms.

Each plant extract in the composition according to the present invention may be included in the following ratio. For example, the weight ratio of Dong Quai extract: Peony extract: Cnidium extract: Sukjihwang extract may be included as 1: 0.1 to 10: 0.1 to 10: 0.1 to 10, 1: 0.1 to 5: 0.1 to 5: 0.1 to 5, 1: 0.1 to 3: 0.1 to 3: 0.1 to 3, 1: 0.1 to 2: 0.1 to 2: 0.1 to 2, 1: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5.

A composition according to the present invention contains, in its final form, at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012%, 0.0013%, 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.0024%, 0.0025%, 0.0026 %, 0.0027%, 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0 040%, 0.0041%, 0.0042%, 0.0043%, 0.0044%, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%, 0.0063%, 0.0064%, 0.0065%, 0.0066%, 0.0067 %, 0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077%, 0.0078%, 0.0079%, 0.0080%, 0.0 081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.0094%, 0.0095%, 0.0096%, 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0.0400 %, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0 750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000%, 0.2250%, 0.2500%, 0.2750%, 0.3000%, 0.3250%, 0.3500%, 0.3750%, 0.4000%, 0.4250%, 0.4500%, 0.4750%, 0.5000%, 0.5250 %, 0.550%, 0.5750%, 0.6000%, 0.6250%, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.87 50%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3% , 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, 4 .4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4% , 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9%, 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8 .5%, 8.6%, 8.7%, 8.8%, 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90 %, 95% or 99% or more of one or more extracts of the present invention. The % may be calculated based on the total weight of the composition or the total volume of the composition, and the concentration may be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.

Compositions of the present invention may be formulated into all types of vehicles. Examples of suitable vehicles include, but are not limited to, emulsions (e.g., oil in water, water in oil, silicone in water, water in silicone, water in oil in water, oil in water, oil in water in oil, oil in water in silicone, etc.), creams, lotions, solutions (e.g., aqueous and hydro-alcoholic solutions), anhydrous bases (e.g., lipsticks and powders), gels, ointments, pastes, milks, liquids, aerosols, solid forms, or eye jellies.

Compositions of the present invention may also be encapsulated for delivery to a target area such as the skin. Encapsulation techniques may include, but are not limited to, the use of liposomes, vesicles, and/or nanoparticles (including, for example, biodegradable and non-biodegradable colloidal particles comprising polymeric materials into which an ingredient is trapped, encapsulated, and/or absorbed, such as nanospheres and nanocapsules), which can be used as a delivery vehicle to deliver ingredients to the skin, for example.

The composition according to the present invention can be variously commercialized. For example, it can be commercialized as a cosmetic composition, a food composition, and the like.

The cosmetic composition may be prepared in the form of, for example, a general emulsified formulation and a solubilized formulation. For example, lotion such as flexible lotion or nutrient lotion, emulsion such as facial lotion, body lotion, cream such as nourishing cream, moisture cream, eye cream, massage cream, essence, serum, cosmetic ointment, spray, oil gel, gel, pack, sunscreen, makeup base, liquid type, solid type, or spray type foundation, powder, cleansing cream, cleansing lotion, makeup remover such as cleansing oil, cleanser such as cleansing foam, soap, body wash, etc. It is not limited thereto and may be formulated in various forms known in the art.

The cosmetic composition may further include, in addition to the extract according to the present invention, a conventional component known as a component of a cosmetic composition in the art, optionally selected, as long as the object of the present invention is not impaired. For example, fragrances (synthetic and natural), dyes and color ingredients, absorbents, emulsifiers, stabilizers, lubricants, solvents, humectants (including emollients, humectants, film formers, occlusive agents, and agents that affect the skin's natural moisturizing mechanism), water-repellants, UV absorbers (physical and chemical absorbers such as para Aminobenzoic acid ("PABA") and its corresponding PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins (e.g. A, B, C, D, E and K), trace metals (e.g. zinc, calcium and selenium), anti-irritants (e.g. steroid and non-steroidal anti-inflammatory drugs), anti-microbial agents, antioxidants (e.g. BHT and tocopherol), chelating agents (e.g., disodium EDTA and tetrasodium EDTA), preservatives (e.g., methylparaben and propylparaben), pH adjusting agents (e.g., sodium hydroxide and citric acid), absorbents (e.g., aluminum starch octenylsuccinate, kaolin, corn starch, oat starch, cyclodextrins, talc, and zeolites), skin bleaching and lightening agents (e.g., hydroquinone and niacinamide lactate), humectants (eg glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea and mannitol), exfoliants (eg alpha-hydroxy acids and beta-hydroxy acids such as lactic acid, glycolic acid and salicylic acid; and salts thereof), waterproofing agents (eg magnesium/aluminum hydroxy de stearate), skin conditioning agents (e.g., aloe extract, allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate), and thickening agents (e.g., substances capable of increasing the viscosity of the composition, such as carboxylic acid polymers, crosslinked polyacrylate polymers, poly acrylamide polymers, polysaccharides and gums) and silicone-containing compounds such as silicone oils and polyorganosiloxanes.

The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc.), but is not limited thereto. Such supplements may include vitamins, minerals, herbs or other botanicals, amino acids, enzymes and metabolites. These adjuvants are suitable for oral consumption and can be administered orally.

It may be provided as a kit comprising a composition according to the present invention. The composition according to the present invention is contained in a container, which may include, but is not limited to, a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressure vessel, a barrier container, a package, a compartment, a lipstick container, a compact container, a cosmetic pan capable of containing the cosmetic composition, or other types of containers such as, but not limited to, a dispersion or composition or preferred bottle, dispenser, or plastic container that is injected or blow-molded into a package. The kit may include instructions for using the kit or composition, and the instructions may be written on a separate sheet or may be written on the surface of the container or the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures, or symbols. Instructions may include, for example, instructions on how to use, apply, and maintain the kit or composition. The containers may be distributed according to a pre-determined amount.

Compositions according to the present invention may be provided as topical skin compositions.

In addition, a method of topically applying the composition according to the present invention to the skin can be provided.

In the present invention, the term "topical application" means applying or applying a composition onto the surface of keratinous tissue, and "topical skin composition" includes a composition suitable for topical application or application onto keratinous tissue. Such compositions are typically dermatologically acceptable in that they do not exhibit excessive toxicity, incompatibility, instability, allergic reactions, etc. when applied to the skin. Topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.

[Skin improvement effect]

The composition according to the present invention has biological activity and exhibits excellent effects on skin improvement.

More specifically, the composition according to the present invention has a skin whitening effect.

The skin whitening refers to inhibiting melanin production by inhibiting the melanin formation process.

The present inventors measured the amount of melanin produced by treating the complex extract according to the present invention to melanoma cells, and as a result, it was confirmed that the melanin production inhibitory effect was increased compared to the case of treating each extract, and the skin whitening effect was known. It was found to exert an effect corresponding to arbutin. It was confirmed that the composition according to the present invention has an excellent melanin production inhibitory effect and exhibits an excellent whitening effect.

In addition, the composition according to the present invention has a trouble improving effect.

The trouble refers to symptoms such as skin irritation and inflammation, and the inflammatory reaction is characterized by pain, fever, redness, swelling, loss of function, etc. caused by external stimuli. Histologically, complex symptoms including dilatation accompanied by increased permeability and blood flow of arterioles, capillaries, and venules, exudation of plasma containing plasma proteins, migration of leukocytes to inflamed areas, etc. are observed, and skin irritation reactions are characterized by erythema, itching, fever, etc.

The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and inflammatory reaction, or alleviating the skin irritation reaction or inflammatory reaction that is already in progress.

The present inventors measured the NO production inhibitory effect by treating the composite extract according to the present invention, and as a result, it was confirmed that the NO production inhibitory effect was increased compared to the case of treating each extract, and the NO production inhibitory effect was known. It was found to exert an effect corresponding to L-NMMA. It was confirmed that the composition according to the present invention exhibits an excellent skin trouble improvement effect through inhibition of NO production.

In addition, the composition according to the present invention has an anti-glycation effect.

The glycation refers to a phenomenon in which proteins are destroyed by binding sugars and proteins caused by the presence of excessive sugars. When glycation progresses, it affects collagen and elastin, causing various skin damages including loss of skin elasticity.

As a result of measuring the antiglycation effect by treating the composite extract according to the present invention, the present inventors confirmed that the antiglycation effect was increased compared to the case of treating each extract, and it was found that the antiglycation effect exhibited an effect corresponding to aminoguanidine known. As a result, it was confirmed that the composition according to the present invention exhibits an excellent antiglycation effect.

The complex extract according to the present invention is safe for the skin and has excellent effects on skin improvement including anti-glycation effect, skin whitening effect and skin trouble improvement effect.

Hereinafter, examples and experimental examples will be described in detail to explain the present invention in detail. However, the examples and experimental examples according to the present invention may be modified in many different forms, and the scope of the present invention should not be construed as being limited to the examples and experimental examples described below. Examples and experimental examples of the present invention are provided to more completely explain the present invention to those skilled in the art.

< Example 1> Preparation of Angelica Quail Extract

After drying Angelica gigas well and cutting it into pieces, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. Angelica gigas extract was prepared by filtering the chilled extract through a filter having a pore size of 0.2 μm.

< Example 2> Preparation of peony extract

After drying and cutting the peony well, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare a peony extract.

< Example 3> Preparation of cnidium extract

After drying and cutting the cnidium well, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare a cnidium extract.

< Example 4> Preparation of Sukjihwang Extract

After drying and slicing well, 100 g of dry weight was put in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare an extract of Rehmannia glutinosa.

< Example 5> Preparation of Angelica, Peony, Cnidium and Rehmannia Root (4) Mixed Extracts

Angelica, peony, cnidium, and sukjihwang extracts prepared in the form of extracts were injected in equal amounts to prepare mixed extracts of angelica, peony, cnidium, and sukjihwang.

< Experimental example 1> antiglycation effect

In order to confirm anti-glycation efficacy, the present inventors measured glycation inhibitory activity using L-arginine and glucose.

First, 1M L-arginine and 1M glucose were dissolved in 1M phosphate buffer (pH 7.4) and prepared by diluting the sample to 50 ppm using 1M phosphate buffer. 1M L-arginine and 1M phosphate buffer were mixed at a ratio of 1 to 4, and then 80 μl was dispensed into a 96-well plate. Here, 100 μl of 0.01 M aminoguanidine to be used as a positive control and a sample diluted to 50 ppm, respectively, were added thereto. After mixing these samples well, finally, after adding glucose diluted with 1M phosphate buffer so that the final concentration of glucose is 0.1M, the mixture was reacted at 70°C for 4 hours. The degree of glycosylation was measured by measuring the absorbance at 420 nm of the 96-well plate using a spectrophotometer.

The Glycation experimental group of the following formula is an experimental group in which 1M L-arginine and 1M glucose are added to induce glycation. Glycosylation inhibitory activity can be obtained by the following formula. Each experiment was performed 3 times, and the average value was expressed.

[Equation 1]

('Glycation experimental group' in Equation 1 means 'absorbance of the glycation experimental group')

sample Inhibition rate (%) Control (DMSO, 50 ppm) - Positive control group (Aminoguanidine, 55ppm) 54.89 Example 1 38.15 Example 2 41.34 Example 3 47.81 Example 4 43.25 Example 5
(50ppm) 58.29

As can be seen from the results of Table 1, it can be seen that the mixed extract has a more excellent anti-glycation effect compared to aminoguanidine known as an anti-glycation substance.

< Experimental example 2> Melanin production inhibitory effect

In order to confirm the whitening effect through inhibition of melanin production, according to the method described by Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980), the extract was added to a culture medium of B-16 mouse melanoma cells to measure the total amount of melanin. During the experiment, whitening was evaluated at a non-toxic concentration by first evaluating toxicity to melanoma cells of mice. DMSO was used as a negative control and albutin was used as a positive control.

Specifically, the sample was added to the medium so that the final concentration was 100 ppm, and arbutin was added to the medium so that the final concentration was 100 ppm, and then the melanoma cells were cultured for 3 days. Thereafter, the cells were treated with trypsin, detached from the culture vessel, centrifuged, and then melanin was extracted. The detached cells were boiled for 10 minutes in 1 ml of sodium hydroxide solution (1N concentration) to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by the method of expressing the absorbance per unit cell number (1 × 10 ⁶ cell), the total amount of melanin relative to the control group was calculated as an inhibition rate (%), and the results are shown in Table 2 below. Each experiment was performed three times and represented as an average value.

sample melanin production
(abs) Inhibition rate (%) Control (DMSO, 10 ppm) 0.33 - Positive control group (arbutin, 100ppm) 0.228 30.91 Example 1 0.281 14.85 Example 2 0.273 17.34 Example 3 0.290 12.12 Example 4 0.307 6.97 Example 5
(100ppm) 0.241 26.97

As can be seen from the results of Table 2, it was found that the mixed extract exhibited an excellent melanin total amount reduction effect and was useful for whitening purposes.

< Experimental example 3> Anti-inflammatory effect

In order to confirm the anti-inflammatory effect and skin trouble improvement effect, nitric oxide (NO) production inhibition test was performed by the GRIESS method using the RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, which are mouse macrophages, were subcultured several times, placed in a 24-well plate so that 3×10 ⁵ per well, and then cultured for 24 hours. It was then replaced with a cell medium containing the sample at a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), an NO-production inhibitor, was treated as a positive control and cultured for 30 minutes, and 1 μg of LPS (Lipopolysaccharide) was treated as a stimulus and cultured for 24 hours. 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of the GRIESS solution was added and reacted at room temperature for 10 minutes, and the NO inhibition effect was determined by measuring the absorbance at 540 nm. The NO production inhibition rate (%) was calculated using Equation 2 below and shown in Table 3 below.

[Equation 2]

NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} x 100

sample NO production inhibition rate (%) Control (DMSO, 10 ppm) - L-NMMA (positive control, 10 ppm) 41.02 Example 1 31.25 Example 2 32.64 Example 3 35.65 Example 4 29.13 Example 5
(10ppm) 40.09

As can be seen from the results of Table 3, it was found that the mixed extract exhibited excellent activity as a natural substance, although the relative activity was slightly lower when compared to L-NMMA, a representative anti-inflammatory drug.

Claims (4)

A cosmetic composition for improving skin troubles comprising Angelica gigas extract, peony extract, cnidium extract, and sukjihwang extract as active ingredients. The cosmetic composition according to claim 1, wherein the weight ratio of Angelica quai extract: Peony extract: Cnidium extract: Sukjihwang extract is 1: 0.1 to 10: 0.1 to 10: 0.1 to 10. The cosmetic composition according to claim 1 or 2, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohol, or mixtures thereof. The cosmetic composition according to claim 1, wherein the cosmetic composition has an anti-glycation effect.