KR102474049B1 - Composition for improving skin condition comprising herb extracts mixture - Google Patents

Abstract

The present invention relates to a composition comprising, as an active ingredient, a composite herbal medicine extract that is safe when applied to the skin and has excellent skin improvement effects. More specifically, the composition according to the present invention contains sukjihwang extract, saengjihwang extract, angelica extract, white peony extract, astragalus extract, macmundong extract, cheonmundong extract, jajiryeo extract, yagyo etc. extract, horseback riding extract, seaweed extract and safflower extract as active ingredients As a composition comprising, it has excellent effects on skin improvement, including antiglycation effect, skin whitening effect, skin elasticity and wrinkle improvement effect, antioxidant effect and skin trouble improvement effect.

Description

Composition for improving skin condition comprising herb extracts mixture

The present invention relates to a composition comprising, as an active ingredient, a composite herbal medicine extract that is safe when applied to the skin and has excellent skin improvement effects.

The skin is an important organ that protects the human body from the external environment and is in charge of various physiological functions such as a barrier function that prevents internal moisture and useful components from leaking out, a body temperature control function, and an excretion function. However, the activity of skin cells is reduced and the skin condition is deteriorated due to the following reasons.

Collagen and elastin present in the dermal layer of the skin have a great effect on the components of the skin by performing functions such as mechanical robustness of the skin, resistance of connective tissue, maintenance of tissue cohesiveness, and support of cell adhesion. It is known that such collagen is reduced by stress, aging, or photoaging by ultraviolet irradiation, while elastin is distorted in its three-dimensional structure by the degrading enzyme elastase whose activity is increased after exposure to ultraviolet rays. As a result, the skin tissue becomes loose and loses its elasticity.

As the cell function of the epidermis is lowered, the metabolism is not smooth, and the exfoliation of the dead skin cells does not occur easily. When UV rays are applied in a state in which the dead skin cells are excessively accumulated, the elasticity is lowered, resulting in the occurrence of wrinkles. Or, if the sebaceous film is not formed on the epidermis, moisture and sebum secretion decreases and the skin becomes dry, resulting in wrinkles.

When the skin is exposed to UV rays, melanin is synthesized to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through melanosomes. These keratinocytes turn over every 28 days. Thus, it is common for the generated melanin to be lost by keratinocytes every 28 days, but if the skin cell cycle is not well regulated due to stress, skin aging, etc., keratinocytes do not drop out in time, resulting in pigments such as spots, freckles, and age spots. calm will occur.

In the skin, sebum, sweat components, and cosmetic ingredients are decomposed into highly toxic substances by skin flora, which can cause skin irritation and inflammation. In addition, irritation caused by ultraviolet rays may cause skin trouble by increasing the production of nitric oxide (NO), an inflammatory mediator in the skin. Nitric oxide is mostly produced by iNOS, and iNOS is known to be rapidly induced by stimuli such as LPS and cytokines to produce excess NO.

Reactive oxygen species (ROS), also called toxic oxygen species, are toxic substances produced in cells by physiological processes such as respiration and are constantly produced and destroyed. Under normal conditions, they exist at about 3-5%. . These reactive oxygen species are free radicals such as superoxide radical (O2-) and hydroxyl radical (HO+): atoms or molecules that are chemically unpaired in the outermost electron orbital. It exists in the form of a very unstable and highly reactive form) or in the form of a compound with paired electrons such as hydrogen peroxide (H ₂ O ₂ ) or singlet oxygen (Singlet radical). Reactive oxygen species have the advantage of having a biological defense function of sterilizing bacteria in the physiological system, but generally cause harmful effects that cause diseases by causing oxidation in vivo. It has been reported that these reactive oxygen species attack biomolecules, damage cells or tissues, and cause various diseases related to aging or various adult diseases.

Research has been conducted to suppress skin aging caused by the above-mentioned factors, to improve whitening, wrinkles, elasticity or trouble, and to develop substances having antioxidant effects.

Substances having the above effects are widely distributed in the natural world, and have been mainly used as raw materials for foods, cosmetics, and pharmaceuticals using substances derived from plants. However, such materials derived from nature do not have significant effects and must be used in large amounts to obtain significant effects, resulting in toxicity and price increase problems.

In order to solve these problems of natural materials, chemically synthesized materials have been developed. These have the advantage of exerting a much superior effect even with a small amount compared to natural substances, but on the other hand, there is a fatal problem that can cause large and small side effects in the human body, so their use is limited.

Accordingly, there is an urgent need to develop a substance derived from a natural product and ensuring stability while exhibiting an excellent skin improvement effect.

KR 1020060092179 A KR 1020090002682 A

The problem to be solved by the present invention is to provide a complex herbal extract derived from natural products and ensuring stability while exhibiting excellent effects on skin improvement, in particular, whitening, wrinkles, elasticity, trouble improvement, and antioxidation.

In order to solve the above problems, the present invention is an active ingredient of sukjihwang extract, saengjihwang extract, angelica extract, white peony extract, astragalus extract, macmundong extract, cheonmundong extract, jajiryeo extract, yagyo extract, horseback riding extract, seaweed extract and safflower extract Provides a composition for skin whitening, wrinkle improvement, elasticity improvement, trouble improvement or antioxidant (eg, cosmetic composition) comprising a.

[extract]

Sukjihwang (熟地黃) is steamed and dried roots of Rehmannia glutinosa (Gaertner) Liboschitz ex Steudel of the Scrophulariaceae family, and is known to be effective in darkening hair color and recovering from fatigue.

Rehmanniae Radix Recens is a fresh root of Rehmannia glutinosa Liboschitz ex Steudel, a member of the Scrophulariaceae family.

Angelica Gigas belongs to the Apiaceae family, contains the root of Angelica Gigas Nakai , and has the property of making the five organs healthy and warming the body. has the effect of purifying blood.

Peony (芍藥) includes white peony, peony ( Paeonia lactiflora Pallas) or other related plants of the same genus (Paeoniaceae).

Astragalus is the root of Astragalus membranceus Bunge, Mongolian Astragalus membranaceus Bunge var. will be.

Macmundong (麥門冬) is a perennial herbaceous plant belonging to the Liliaceae family and is the ampulla of the root of Liriope platyphylla Wang et Tang or Ophiopogon japonicus Ker-Gawler.

Cheonmundong (天門冬, Asparagus cochinchinensis Merrill) is a perennial herbaceous plant belonging to the Liliaceae family and grows a lot in the southern coastal regions of Korea.

Zygophyllaceae ( Tribulus ) of the Zygophyllaceae terrestris It is the fruit of Linne.

It is a vine of Polygonum multiflora (Thunberg ex Murray) Haraldson var. multiflora belonging to Polygonaceae.

Horseback riding (升麻, Cimicifugae Rhizoma) is a perennial herb of the family Ranunculaceae that grows in forests. heracleifolia Komarov), candlestick riding ( Cimicifuga simplex Wormskjord), snow riding ( Cimicifuga dahurica Maximowicz) or stork riding (Cimicifuga foetida Linne).

Taoin (桃仁) is a peach tree ( Prunus ) of the Rosaceae family. persica Batsch) or Prunus davidiana Franchet.

Safflower ( Carthamus) tinctorius ) is a biennial herb belonging to the Asteraceae (Compositae), safflower ( Carthamus) It is a flower of tinctorius Linne).

The present inventors have found that all of the above extracts have biological activity beneficial to the skin, and two or more complex extracts of the above extracts, preferably Sukjihwang extract, Saengjihwang extract, Angelica extract, White peony extract, Astragalus extract, Macmundong extract, Cheonmundong extract, Jajiryeo extract , it was found that the composite extracts of Yagyo deung extract, Horseback riding extract, Taoin extract and Safflower extract had synergistic biological activities.

In the present invention, the term "extract" refers to an extract obtained by the extraction treatment, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, etc., and the extract itself. and extracts of all formulations that can be formed using the extract solution.

The extract describes each plant and may include its leaves, stems, barks, roots, flowers or flower buds, fruits, seeds, sap and whole plants in addition to the specified parts.

To prepare the extract, one skilled in the art can use any suitable method known in the art. For example, it may be by a solvent extraction method. A solvent extract can be obtained by crushing the whole plant or any part thereof (eg, in a blender) and then treating the extraction solvent. It may be pulverized after undergoing a drying process (for example, drying at 40 to 70° C. for 15 to 50 hours) before pulverization. In addition, the solvent extract can be prepared in a powder state by additional processes such as distillation under reduced pressure and lyophilization or spray drying.

The type of extraction solvent used is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, and preferably, water and lower alcohol may be used alone or in combination of two or more. A solvent extract may be prepared by extracting at least once using the solvent, and a dried extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray-drying may be prepared.

The amount of the extraction solvent may vary depending on the type of extraction solvent used, but may be used, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.

In addition, various extraction processes known in the art, such as maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous-alcoholic extraction , countercurrent extraction, microwave-assisted extraction, ultrasonic extraction, supercritical fluid extraction, phytonic extract (eg, hydro-fluoro-carbon solvent), etc.) may be selected and used, and these may be performed alone or Two or more methods may be used in combination.

[composition]

The composition according to the present invention contains sukjihwang extract, saengjihwang extract, angelica extract, white peony extract, astragalus extract, macmundong extract, cheonmundong extract, jajilyeo extract, yagyo extract, horseback riding extract, seaweed extract and safflower extract as active ingredients.

In the present invention, the meaning of "included as an active ingredient" means that an extract is added to an extent that can exhibit a skin improvement effect from the composition according to the present invention, and various ingredients are added as subcomponents for skin delivery and stabilization. This means that it can be formulated in various forms.

Each plant extract in the composition according to the present invention may be included in the following ratio. For example, Sukjihwang extract: Saengjihwang extract: Angelica extract: White peony extract: Astragalus extract: Macmundong extract: Cheonmundong extract: Jajiryeo extract: Yagyodeung extract: Horseback riding extract: Taoist extract: Safflower extract The weight ratio is 1: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10, which can be included as 1: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5, 1: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3, 1: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2, 1: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5.

A composition according to the present invention comprises at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012%, 0.0013% in its final form. 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.0024%, 0.0025%, 0.0026%, 0.0027%, 0.0028%, 0.0029%, 0.0030 %, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042%, 0.0043%, 0.00444%, 0.0043%, 0.0043%, 0.0044% 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%, 0.0063% 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077%, 0.0078%, 0.0079%, 0.0080 %, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.090.60%, 0.090.60%, 0.5094% 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0.040 0%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.5%, 0.0725%, 0.070.50% , 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000%, 0.2250% %, 0.3500%, 0.3750%, 0.4000%, 0.4250%, 0.4500%, 0.4750%, 0.5000%, 0.5250%, 0.550%, 0.5750%, 0.6000%, 0.6250%, 0.6500%, 0.6750%, 0.6750%, 0.6750% 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.6%, 1.5% , 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3 %, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6% , 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9%, 8.0%, 8.1%, 8.2%, 8.3 %, 8.4%, 8.5%, 8.6%, 8.7% , 8.8%, 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14 %, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99% or more of one or more extracts of the present invention. can The % may be calculated based on the total weight of the composition or the total volume of the composition, and the concentration may be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.

Compositions of the present invention may be formulated into all types of vehicles. Examples of suitable vehicles include emulsions (e.g., oil in water, water in oil, silicone in water, water in silicone, water in oil in water, oil in water, oil in water in oil, oil in water in silicone, etc.), creams, lotions, solutions (e.g., aqueous and hydro-alcohol solutions), anhydrous bases (eg lipsticks and powders), gels, ointments, pastes, milks, liquids, aerosols, solid forms or eye jellies.

Compositions of the present invention may also be encapsulated for delivery to a target area such as the skin. Encapsulation technologies include, for example, liposomes, vesicles, and/or nanoparticles that can be used as delivery vehicles to deliver ingredients to the skin (e.g., polymeric materials into which ingredients are trapped, encapsulated, and/or absorbed). biodegradable and non-biodegradable colloidal particles including, for example, nanospheres and nanocapsules).

The composition according to the present invention can be variously commercialized. For example, it can be commercialized as a cosmetic composition, a food composition, and the like.

The cosmetic composition may be prepared in the form of, for example, a general emulsified formulation and a solubilized formulation. For example, lotion such as softening lotion or nourishing lotion, emulsion such as facial lotion, body lotion, etc., cream such as nourishing cream, moisture cream, eye cream, massage cream, essence, serum, makeup ointment, spray, oil gel, gel , packs, sunscreens, makeup bases, foundations such as liquid, solid or spray types, powders, cleansing creams, cleansing lotions, makeup removers such as cleansing oils, cleansers such as cleansing foams, soaps, body washes, etc. It may have, but is not limited thereto and may be formulated in various forms known in the art.

The cosmetic composition may further include, in addition to the extract according to the present invention, a conventional component known as a component of a cosmetic composition in the art, optionally selected, as long as the object of the present invention is not impaired. For example, fragrances (synthetic and natural), dyes and color ingredients, absorbents, emulsifiers, stabilizers, lubricants, solvents, humectants (e.g. emollients, humectants, film formers, occlusives) agents), and agents that affect the skin's natural moisturizing mechanism), water-repellants, UV absorbers (physical and chemical absorbers such as paraaminobenzoic acid ("PABA") and PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins (eg A, B, C, D, E and K), trace metals (eg zinc, calcium and selenium), anti- Anti-irritants (e.g. steroid and non-steroidal anti-inflammatory drugs), anti-microbial agents, antioxidants (e.g. BHT and tocopherol), chelating agents (e.g. disodium EDTA and tetrasodium EDTA) , preservatives (e.g. methylparaben and propylparaben), pH adjusters (e.g. sodium hydroxide and citric acid), absorbents (e.g. aluminum starch octenylsuccinate, kaolin, corn starch, oat starch, cyclodextrin, talc). ), and zeolites), skin bleaching and lightening agents (eg hydroquinone and niacinamide lactate), humectants (eg glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol , urea and mannitol), exfoliants (eg alpha-hydroxy acids and beta-hydroxy acids such as lactic acid, glycolic acid and salicylic acid; and salts thereof), waterproofing agents (eg magnesium/aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extract, allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium)glycyrrhizates), and thickening agents (e.g., substances capable of increasing the viscosity of the composition, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums) and silicone-containing compounds (eg, silicone oil and polyorganosiloxane).

The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc.), Not limited to this. Such supplements may include vitamins, minerals, herbs or other botanicals, amino acids, enzymes and metabolites. These adjuvants are suitable for oral consumption and can be administered orally.

It may be provided as a kit comprising a composition according to the present invention. The composition according to the present invention is contained in a container, the container may contain a bottle, metal tube, laminate tube, plastic tube, dispenser, pressure container, barrier container, package, compartment, lipstick container, compact container, cosmetic composition cosmetic pans, or other types of containers, such as, but not limited to, plastic containers that are injected or blow-molded into a preferred bottle, dispenser, or package in which dispersions or compositions are held. . The kit may include instructions for using the kit or composition, and the instructions may be written on a separate sheet or may be written on the surface of the container or the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures, or symbols. Instructions may include, for example, instructions on how to use, apply, and maintain the kit or composition. The containers may be distributed according to a pre-determined amount.

Compositions according to the present invention may be provided as topical skin compositions.

In addition, a method of topically applying the composition according to the present invention to the skin can be provided.

In the present invention, the term "topical application" means applying or applying a composition onto the surface of keratinous tissue, and "topical skin composition" includes a composition suitable for topical application or application onto keratinous tissue. Such compositions are typically dermatologically acceptable in that they do not exhibit excessive toxicity, incompatibility, instability, allergic reactions, etc. when applied to the skin. Topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.

[Skin improvement effect]

The composition according to the present invention has biological activity and exhibits excellent effects on skin improvement.

More specifically, the composition according to the present invention has a skin whitening effect.

The skin whitening refers to inhibiting melanin production by inhibiting the melanin formation process.

The present inventors measured the amount of melanin produced by treating melanoma cells with the complex extract according to the present invention, and as a result, it was confirmed that the melanin production inhibitory effect was increased compared to the case of treating each extract, and skin whitening It was found that the effect was equivalent to that of arbutin, which is known. It was confirmed that the composition according to the present invention has an excellent melanin production inhibitory effect and exhibits an excellent whitening effect.

In addition, the composition according to the present invention has an effect of improving wrinkles and elasticity.

The skin wrinkles refer to fine lines caused by skin deterioration, and may be caused by genes, reduction of collagen and elastin present in the skin dermis, external environment, and the like.

The wrinkle improvement refers to suppressing or inhibiting the formation of wrinkles or alleviating wrinkles already formed.

The skin elasticity is represented by elastic fibers composed of elastin and collagen fibers present in the dermal layer, and the improvement of elasticity refers to suppressing or inhibiting a decrease in elasticity, maintaining or improving elasticity. .

As a result of treating the complex extract according to the present invention, the present inventors confirmed that the collagen synthesis promoting effect and the elastase activity inhibitory effect were increased compared to the case of treating each extract, and the skin collagen synthesis promoting effect was known. It was found that it exhibits an effect corresponding to vitamin C, which is known, and an effect corresponding to quercetin, which has a known skin elastase activity inhibitory effect. It was confirmed that the composition according to the present invention has an excellent collagen synthesis promoting effect and an elastase activity inhibiting effect, and exhibits excellent skin wrinkle and elasticity improvement effects.

In addition, the composition according to the present invention has a trouble improving effect.

The trouble refers to symptoms such as skin irritation and inflammation, and the inflammatory response is characterized by pain, fever, redness, swelling, loss of function, etc. caused by external stimulation, and histologically, the permeability and blood flow of arterioles, capillaries, and venules Complex symptoms, including expansion accompanied by increase, exudation of plasma containing plasma proteins, migration of leukocytes to inflamed areas, etc. are observed, and skin irritation reactions are characterized by erythema, itching, and fever.

The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and inflammatory reaction, or alleviating the skin irritation reaction or inflammatory reaction that is already in progress.

The present inventors measured the NO production inhibitory effect by treating the complex extract according to the present invention, and as a result, it was confirmed that the NO production inhibitory effect was increased compared to the case of treating each extract, and the NO production inhibitory effect was known. It was found that it exerted an effect corresponding to L-NMMA. It was confirmed that the composition according to the present invention exhibits an excellent skin trouble improvement effect through inhibition of NO production.

In addition, the composition according to the present invention has an antioxidant effect.

The antioxidant refers to inhibiting oxidation of cells by highly reactive free radicals or reactive oxygen species (ROS) according to intracellular metabolism or oxidative stress caused by the influence of ultraviolet rays, and free radicals or removing reactive oxygen species, thereby reducing cell damage.

The present inventors measured the free radical scavenging activity by treating the complex extract according to the present invention, and as a result, it was confirmed that the free radical scavenging activity was increased compared to the case of treating each extract, and vitamin C, which is known to have free radical scavenging activity. It was found to exert a corresponding effect. Thus, it was confirmed that the composition according to the present invention exhibits an excellent antioxidant effect.

In addition, the composition according to the present invention has an anti-glycation effect.

The glycation refers to a phenomenon in which proteins are destroyed by binding sugars and proteins caused by the presence of excessive sugars. When glycation progresses, it affects collagen and elastin, causing various skin damages including loss of skin elasticity.

As a result of measuring the antiglycation effect by treating the complex extract according to the present invention, the present inventors confirmed that the antiglycation effect was increased compared to the case of treating each extract, and aminoguanidine, whose antiglycation effect is known It was found to exert a corresponding effect. As a result, it was confirmed that the composition according to the present invention exhibits an excellent antiglycation effect.

The complex extract according to the present invention is safe for the skin and has excellent effects on skin improvement including antiglycation effect, skin whitening effect, skin elasticity and wrinkle improvement effect, antioxidant effect and skin trouble improvement effect.

Hereinafter, examples and experimental examples will be described in detail to explain the present invention in detail. However, the examples and experimental examples according to the present invention may be modified in many different forms, and the scope of the present invention should not be construed as being limited to the examples and experimental examples described below. Examples and experimental examples of the present invention are provided to more completely explain the present invention to those skilled in the art.

< Example 1> Manufacture of Sukjihwang extract

After drying and slicing well, 100 g of dry weight was put in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare an extract of Rehmannia glutinosa.

< Example 2> Preparation of saengjihwang extract

After cutting raw Rehmannia Root, 100 g of weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare a Saengjihwang extract.

< Example 3> Preparation of Angelica Quail Extract

After drying Angelica gigas well and cutting it into pieces, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. Angelica gigas extract was prepared by filtering the chilled extract through a filter having a pore size of 0.2 μm.

< Example 4> Preparation of white peony extract

After drying and cutting the white peony well, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare an extract of White Peony.

< Example 5> Preparation of Astragalus Extract

After drying and cutting Astragalus well, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. Astragalus extract was prepared by filtering the chilled extract through a filter having a pore size of 0.2 μm.

< Example 6> Manufacture of Macmundong Extract

After drying well and slicing, 100 g of dried weight was put in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare an extract of Macmundong.

< Example 7> Cheonmundong Preparation of extract

After drying and cutting the cheonmundong well, 100 g of dry weight was put in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. Cheonmundong extract was prepared by filtering the chilled extract through a filter having a pore size of 0.2 μm.

< Example 8> Manufacture of self-extract

After drying well and chopping, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare an extract.

< Example 9> night school Preparation of extract

After drying well and cutting the yagyo etc., 100 g of dry weight was put in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare an extract of yagyo etc.

< Example 10> Preparation of Horse Riding Extract

After drying and cutting the horsetail well, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare horseback riding extract.

< Example 11> Preparation of Perilla extract

After drying well and cutting the seaweed, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare an extract of seaweed.

< Example 12> Preparation of safflower extract

After drying the safflower well and cutting it into pieces, 100 g of dry weight was placed in a flask and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare a safflower extract.

< Example 13> Sukjihwang, Saengjihwang, Angelica, White Peony, Astragalus, Maekmundong, Cheonmundong , take care , Preparation of mixed extracts of yagyo deung, horse riding, Taoin and safflower (12 types)

As in the above-described embodiment, the same amount of extracts of sukjihwang, saengreh, angelica, white peony, astragalus, maekmundong, cheonmundong, jajiryeo, yagyo, horseback riding, swordfish, and safflower extracts prepared in the form of extracts were injected, Mixed extracts of Astragalus, Maekmundong, Cheonmundong, Jajiryeo, Yagyodeung, Horse riding, Daoin and Safflower extracts were prepared.

< Experimental example 1> antiglycation effect

In order to confirm anti-glycation efficacy, the present inventors measured glycation inhibitory activity using L-arginine and glucose.

First, 1M L-arginine and 1M glucose were dissolved in 1M phosphate buffer (pH 7.4) and prepared by diluting the sample to 50 ppm using 1M phosphate buffer. 1M L-arginine and 1M phosphate buffer were mixed at a ratio of 1 to 4, and then 80 μl was dispensed into a 96-well plate. Here, 100 μl of 0.01 M aminoguanidine to be used as a positive control and a sample diluted to 50 ppm, respectively, were added thereto. After mixing these samples well, finally, after adding glucose diluted with 1M phosphate buffer so that the final concentration of glucose is 0.1M, the mixture was reacted at 70°C for 4 hours. The degree of glycosylation was measured by measuring the absorbance at 420 nm of the 96-well plate using a spectrophotometer.

The Glycation experimental group of the following formula is an experimental group in which 1M L-arginine and 1M glucose are added to induce glycation. Glycosylation inhibitory activity can be obtained by the following formula. Each experiment was performed 3 times, and the average value was expressed.

[Equation 1]

('Glycation experimental group' in Equation 1 means 'absorbance of the glycation experimental group')

sample Inhibition rate (%) Control (DMSO, 50 ppm) - Positive control group (Aminoguanidine, 55ppm) 54.89 Example 1 41.78 Example 2 44.94 Example 3 50.11 Example 4 50.49 Example 5 48.33 Example 6 50.12 Example 7 51.32 Example 8 49.18 Example 9 49.84 Example 10 48.43 Example 11 51.58 Example 12 40.55 Example 13 (50 ppm) 59.15

As can be seen from the results of Table 1, it can be seen that the mixed extract has a more excellent anti-glycation effect compared to aminoguanidine known as an anti-glycation substance.

< Experimental example 2> Melanin production inhibitory effect

In order to confirm the whitening effect through inhibition of melanin production, according to the method described by Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980), in a culture medium of B-16 mouse melanoma cells, The total amount of melanin was measured by adding the extract. During the experiment, whitening was evaluated at a non-toxic concentration by first evaluating toxicity to melanoma cells of mice. DMSO was used as a negative control and albutin was used as a positive control.

Specifically, the sample was added to the medium so that the final concentration was 100 ppm, and arbutin was added to the medium so that the final concentration was 100 ppm, and then the melanoma cells were cultured for 3 days. Thereafter, the cells were treated with trypsin, detached from the culture vessel, centrifuged, and then melanin was extracted. The detached cells were boiled for 10 minutes by adding 1 ml of sodium hydroxide solution (1N concentration) to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by the method of expressing the absorbance per unit cell number (1×10 ⁶ cell), the total amount of melanin relative to the control group was calculated as an inhibition rate (%), and the results are shown in Table 2 below. Experiments were performed three times each. It was performed and expressed as an average value.

sample melanin production
(abs) Inhibition rate (%) Control (DMSO, 10 ppm) 0.33 - Positive control group (Arbutin, 100ppm) 0.228 30.91 Example 1 0.285 13.64 Example 2 0.301 8.79 Example 3 0.318 3.64 Example 4 0.292 11.52 Example 5 0.290 12.12 Example 6 0.302 8.48 Example 7 0.298 9.70 Example 8 0.288 12.73 Example 9 0.301 8.79 Example 10 0.253 23.33 Example 11 0.307 6.97 Example 12 0.286 13.33 Example 13
(100ppm) 0.267 19.09

As can be seen from the results of Table 2, it was found that the mixed extract exhibited an excellent melanin total amount reducing effect and was useful for whitening purposes.

< Experimental example 3> Anti-inflammatory effect

In order to confirm the anti-inflammatory effect and skin trouble improvement effect, nitric oxide (NO) production inhibition test was performed by the GRIESS method using the RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, which are mouse macrophages, were subcultured several times, placed in a 24-well plate so that 3×10 ⁵ per well, and then cultured for 24 hours. It was then replaced with a cell medium containing the sample at a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), an NO-production inhibitor, was treated as a positive control and cultured for 30 minutes, and 1 μg of LPS (Lipopolysaccharide) was treated as a stimulus and cultured for 24 hours. 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of the GRIESS solution was added and reacted at room temperature for 10 minutes, and the NO inhibition effect was determined by measuring the absorbance at 540 nm, and the NO production inhibition rate (%) was as follows It was calculated using Equation 2 and shown in Table 3 below, and the experiment was performed three times each, and the average value was shown.

[Equation 2]

NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} x 100

sample NO production inhibition rate (%) Control (DMSO, 10 ppm) - L-NMMA (positive control, 10 ppm) 41.02 Example 1 30.15 Example 2 31.51 Example 3 30.95 Example 4 35.49 Example 5 28.15 Example 6 29.45 Example 7 29.75 Example 8 30.19 Example 9 37.56 Example 10 33.25 Example 11 26.15 Example 12 24.10 Example 13
(10ppm) 39.79

As can be seen from the results of Table 3, it was found that the mixed extract exhibited excellent activity as a natural substance, although the relative activity was slightly lower when compared to L-NMMA, a representative anti-inflammatory drug.

< Experimental example 4> Collagen synthesis promotion effect

The sample was added to the culture medium of human-derived fibroblasts to confirm the effect of promoting type 1 collagen synthesis at the cellular level. The measurement of synthesized collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT). In order to measure the amount of collagen synthesis, the sample was added to the culture medium (DMEM medium) of fibroblasts to a final concentration of 10 ppm, cultured for 48 hours, and then the culture medium was taken and the degree of type 1 collagen synthesis at each concentration was measured using the PICP EIA kit. It was measured at 450 nm using a spectrophotometer.

For comparison of the effect, the degree of collagen synthesis was measured in the same manner for the samples to which the culture medium of untreated fibroblasts (negative control) and vitamin C (positive control) were added to a final concentration of 52.85 μg/ml. The rate of increase in collagen production was calculated as the ratio of the amount of collagen production relative to the negative control group, and the results are shown in Table 4 below. The experiment was performed 4 times and presented as the average value.

sample Type 1 collagen production (ng/ml) Increase (%) negative control group 150.2 - Positive control group (vitamin C) 246.8 64.3 Example 1 218.5 45.5 Example 2 202.1 34.6 Example 3 218.5 45.5 Example 4 198.7 32.3 Example 5 157.4 4.8 Example 6 186.2 24.0 Example 7 180.9 20.44 Example 8 198.5 32.2 Example 9 179.4 19.4 Example 10 210.3 40.0 Example 11 168.5 12.2 Example 12 178.1 18.6 Example 13
(10ppm) 239.8 59.7

As can be seen from the results of Table 4, when the mixed extract was treated, collagen synthesis was increased, and in general, the collagen synthesis effect was similar to that of vitamin C, which is well known to induce collagen synthesis.

< Experimental example 5> Elastase active inhibitory effect

The effect of inhibiting the activity of elastase, an enzyme that degrades elastin, was confirmed as follows.

Elastase derived from human leukocytes was used, and a synthetic substrate MeOSuc-Ala-Ala-Pro-Val-pNA was used as a substrate for the elastase. A 100 mM Tris (pH 7.5) solution was used as the buffer solution. Elastase was finally used at 0.2 mU using a buffer solution. In addition, the synthetic substrate of elastase was diluted with a buffer solution to a final concentration of 0.5 mM after making a 100 mM solution using DMSO. At this time, as a positive control group, Quercetin, known as an elastase inhibitor, was set at a concentration of 10 ppm. The elastase inhibitory candidate was added to a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and reacted at room temperature for 20 minutes. The absorbance was measured at 405 nm at 1-minute intervals using a spectrophotometer, and the slope of the absorbance versus time was determined to determine the activity of the enzyme. The elastase inhibition rate was calculated as follows.

[Equation 3]

The elastase inhibition rate was calculated using Equation 3 and shown in Table 5 below.

sample enzyme activity Inhibition rate (%) Example 1 5.3 49.03 Example 2 6.2 40.38 Example 3 4.2 59.62 Example 4 4.9 52.88 Example 5 5.1 50.96 Example 6 5.5 47.11 Example 7 5.2 50.00 Example 8 7.9 24.04 Example 9 6.2 40.38 Example 10 5.2 50.00 Example 11 6.5 37.50 Example 12 5.7 45.19 Example 13
(10ppm) 4.0 61.54 Positive control group (Quercetin, 10ppm) 3.5 66.35 Control (DMSO, 20 ppm) 10.4 -

As can be seen from the results of Table 5, when the mixed extract was treated, it was found to exert an excellent elastase activity inhibitory effect even compared to the positive control, quercetin. Therefore, it was found that the mixed extract can be used for skin regeneration and wrinkle improvement.

< Experimental Example 6> Antioxidant effect

The free radical scavenging ability of the mixed extract was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical and exhibits maximum absorption at 517 nm when present in a radical state, and loses absorbance when the radical is scavenged. DPPH was used from Sigma, and was dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of the mixed extract or vitamin C as a positive control was added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, left at room temperature for 30 minutes, and absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).

Table 6 shows the results of expressing the concentration of the extract as IC ₅₀ when the absorbance of the treated sample becomes half of that of the control group. This experiment was repeated 3 times.

sample IC50 (%w/v) control group 0.0025 Positive control group (vitamin C) concentration 0.0005 Example 1 Concentration 0.0021 Example 2 Concentration 0.0053 Example 3 Concentration 0.0042 Example 4 Concentration 0.0015 Example 5 Concentration 0.0032 Example 6 Concentration 0.0029 Example 7 Concentration 0.0036 Example 8 Concentration 0.0052 Example 9 Concentration 0.0053 Example 10 Concentration 0.0014 Example 11 Concentration 0.0019 Example 12 Concentration 0.0030 Example 13 Concentration 0.0010

As shown in Table 6, the mixed extract had very strong free radical scavenging activity compared to vitamin C, and thus it was confirmed that the antioxidant effect was excellent.

Claims (4)

Skin whitening, wrinkle improvement, and elasticity containing as active ingredients Sukjihwang extract, Saengjihwang extract, Angelica extract, White peony extract, Astragalus extract, Maekmundong extract, Cheonmundong extract, Jajiryeo extract, Yagyoden extract, Horseback riding extract, Taoist artichoke extract, and Safflower extract as active ingredients A cosmetic composition for improvement, trouble improvement or antioxidant. According to claim 1, Sujihwang extract: Saengjihwang extract: Angelica extract: White peony extract: Astragalus extract: Macmundong extract: Cheonmundong extract: Jajiryeo extract: Yagyoden extract: Horseback riding extract: Taoist extract: Safflower extract The weight ratio is 1: 0.1 ~ 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10 Cosmetic composition, characterized in that. The cosmetic composition according to claim 1 or 2, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohol, or mixtures thereof. The cosmetic composition according to claim 1, wherein the cosmetic composition is anti-glycation, inhibits melanin production, promotes collagen synthesis, inhibits elastase activity, or scavenges free radicals.