KR20210131598A - Composition for preventing hair loss or promoting hair growth, comprising Camellia japonica pericarp extract as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or promoting hair growth, comprising a camellia pericarp extract as an active ingredient. More specifically, the present invention relates to a cosmetic composition, a pharmaceutical composition and a food composition, comprising as an active ingredient a camellia pericarp extract that can promote hair growth by inducing the proliferation of hair papilla cells and inhibit the production of dihydrotestosterone by inhibiting the activity of 5-alpha reductase, thereby preventing hair loss and promoting hair growth. The composition according to the present invention has excellent safety to the human body without skin irritation and cytotoxicity and also promotes the proliferation of hair papilla cells by activating the Wnt signaling process, and is very effective in inhibiting the production of dihydrotestosterone, thereby being advantageously used as an active ingredient in a cosmetic composition, a pharmaceutical composition or a food composition for preventing hair loss and promoting hair growth.

Description

Composition for preventing hair loss or promoting hair growth, comprising Camellia japonica pericarp extract as an active ingredient}

The present invention relates to a composition for preventing hair loss or promoting hair growth comprising a camellia peel extract as an active ingredient. More specifically, a camellia peel extract, which promotes hair growth by inducing proliferation of dermal papilla cells, and inhibits the activity of 5-alpha reductase to inhibit dihydrotestosterone production, thereby preventing hair loss and promoting hair growth. It relates to a cosmetic composition, a pharmaceutical composition, and a food composition including as an active ingredient.

As science advances, the causes of chronic diseases and various treatment methods are being announced in many medical fields. In particular, modern society is actively seeking ways to treat psychological problems. Hair loss is not a pathological problem, but it causes aesthetic and psychological stress. In severe cases, it can lead to secondary diseases such as low self-esteem or social phobia.

Hair loss, which was previously thought to be a part of aging, has become one of the causes that have the greatest influence on appearance since it appears at a high rate in young people in modern society. The age of hair loss onset was about 50% in their 20s and 30s, 29.8 years old for men and 33.6 years old for women, and only 224,688 people were treated for hair loss in 2018. Accordingly, the domestic hair loss treatment market is approaching 4 trillion won, including treatments, medical devices, shampoos, and herbal medicines. Causes of hair loss include an unbalanced diet and anemia due to iron deficiency, side effects of medications, use of strong chemical products such as dyes or perms, hormonal changes, decreased thyroid function, stress, hair handling habits, depression, extreme temperature changes Various factors such as changing seasons are known.

Types of hair loss are divided into scarring alopecia, in which the hair is not regenerated due to the destruction of the hair follicles due to scarring, and non-scarring alopecia, in which the hair is regenerated after the symptom site disappears because the hair follicle is maintained without scar formation. The application of hair loss treatment or prevention agents focuses on scarring alopecia. A product that prevents hair loss along with hair growth is called an anti-hair loss agent, and a hair growth agent that supplies nutrients to the hair root to make hair grow, a hair restorer that promotes blood circulation in the scalp to help prevent hair loss, and turns thin soft hair into healthy terminal hair. It is defined as a hair-forming agent (JY Lim et al ., Kor. J. Aesthet. Cosmetol ., 12(6):767-772, 2014).

Androgenetic alopecia, in which male hormones act as an important etiology among scar alopecia, accounts for about 80% of all hair loss symptoms. Genetic factors, the concentration of androgen, the male hormone in the blood, and the high concentration of 5-alpha dihydrotestosterone (DHT), which binds to androgen receptors in the hair follicles of the scalp, are known to be the main causes. DHT regulates hair growth by binding to the androgen receptor in the scalp dermal papilla. The binding of DHT transmits a cell-destructive signal to the gene of keratinocyte cells to produce the apoptosis factors DKK-1 and TGF-β1. Finally, hair loss occurs due to the destruction of hair cells (Inui S et al., J Dermatol Sci ., 61(1):1-6, 2011).

Well-known deodorants include minoxidil for external skin application approved by FDA, finasteride for oral use, and dutasteride approved by Korea's MFDS. Although there is strong speculation that minoxidil will follow the mechanism of supplying nutrients to hair follicles by dilating blood vessels through hyperpolarization of cell membranes, research results also announced that it can activate beta-catenin signaling, a representative proliferation mechanism, by acting directly on human hair follicle cells. (Kwack MH et al., J Dermatol Sci. , 62(3):154-159, 2011). Finasteride and dutasteride have a known mechanism of inhibiting the production of DHT that causes hair loss by inhibiting 5-alpha reductase (5α-Reductase) that changes testosterone to DHT (Yamana K et al., Horm Mol Biol Clin Investig. , 1,2(3):293-299, 2010; Clark RV et al., J Clin Endocrinol Metab. , 89(5):2179-2184, 2004). However, chemical anti-aging agents known as hair loss treatments lose their effectiveness when stopped, so continuous prescription is required. is coming

The main mechanisms of detoxification agents include a method of activating the proliferation of human dermal papilla cells and related signaling mechanisms, and a method of lowering the production rate of DHT through the inhibition of the aforementioned 5-alpha reductase. The dermal papilla cells are not only important for the development and growth of hair follicles, but are also multipotent cells. Hair growth is largely divided into anagen, degenerative, telogen, and developmental phases, and overall hair growth occurs while dermal papilla cells proliferate during the anagen phase (Driskell RR et al., J Cell Sci. , 15;124(Pt8):1179). -1182, 2011). Several factors are involved in the proliferation of dermal papilla cells, including FGF-7, which increases the growth phase, ERK activity, which induces cell growth, beta-catenin mechanism, which leads to the development of hair follicles, and Akt activity, which inhibits apoptosis (Gentile P et al. ., Cells., 16;8(5), doi: 10.3390/cells8050466, 2019).

Camellia japonica ( Camillia japonica ) is an evergreen arboreal tree belonging to the family Cactaceae, and numerous horticultural varieties have been made and most have been used as ornamental trees or as camellia oil extracted from seeds. The camellia flower contains leucoanthocyanin and anthocyanin, which have excellent physiological activity, and the fruit contains fatty oil, camelin, and thachysaponin. Camellia oil, which has been widely used as a hair oil since ancient times, contains about 80% of omega-9 oleic acid, and also contains polyphenols, vitamins A, C, D, E and other antioxidants. is known to do These secondary metabolites are known to have effects such as cardiac action, yanghyeol, hemostasis, live fish, and small bells.

In relation to the camellia effect, Korean Patent No. 10-1672431 discloses the cardiovascular disease prevention effect of camellia extract, and Korean Patent No. 10-1408019 discloses the antioxidant and whitening effect of camellia fruit extract, Korean Patent Registration Nos. 10-1547920 and 10-1452320 disclose the hair loss improvement effect of camellia oil, but there is no information on the hair loss prevention effect of Camellia japonica pericarp extract yet.

Therefore, the present inventors conducted a study to find a natural antioxidant through the activation of the beta-catenin mechanism involved in the proliferation of dermal papilla cells and the development of hair follicles, and the inhibition of 5-alpha reductase. It was found that the present invention was completed accordingly.

Republic of Korea Patent Registration No. 10-1672431 Republic of Korea Patent Registration No. 10-1408019 Republic of Korea Patent No. 10-1547920 Republic of Korea Patent No. 10-1452320

JY Lim et al., Kor. J. Aesthet. Cosmetol., 12(6):767-772, 2014 Inui S et al., J Dermatol Sci., 61(1):1-6, 2011 Kwack MH et al., J Dermatol Sci., 62(3):154-159, 2011 Yamana K et al., Horm Mol Biol Clin Investig., 1,2(3):293-299, 2010 Clark RV et al., J Clin Endocrinol Metab., 89(5):2179-2184, 2004 Driskell RR et al., J Cell Sci., 15;124(Pt8):1179-1182, 2011 Gentile P et al., Cells., 16;8(5), doi: 10.3390/cells8050466, 2019

It is an object of the present invention to provide a cosmetic composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

Another object of the present invention is to provide a food composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

In addition, the present invention provides a food composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

The composition for preventing hair loss or promoting hair growth, containing the extract of the camellia peel of the present invention as an active ingredient, has excellent human safety due to no skin irritation and cytotoxicity, and promotes proliferation of dermal papilla cells by activating the Wnt signal transduction process, and dihydrotestosterone Since it is very effective in inhibiting the production of hair loss, it can be usefully used as an active ingredient in a cosmetic composition, pharmaceutical composition or food composition for preventing hair loss and promoting hair growth.

1 is a graph showing the analysis of the dermal papilla cell proliferation effect by the extract of the camellia peel of the present invention.
Figure 2 is a graph showing the inhibitory effect of 5-alpha reductase activity by the extract of camellia bark of the present invention.
3 is a graph showing the degree of Wnt signaling activation by camellia peel extract of the present invention.

The present invention relates to a composition for preventing hair loss or promoting hair growth comprising a camellia peel extract as an active ingredient. More specifically, it relates to a cosmetic composition, a pharmaceutical composition, and a food composition comprising an ethanol extract of camellia peel as an active ingredient, wherein the composition has excellent human safety due to no skin irritation and cytotoxicity, while activating the Wnt signaling process. By promoting the proliferation of dermal papilla cells and inhibiting the activity of 5-alpha reductase to inhibit the production of dihydrotestosterone, it is possible to effectively prevent hair loss and promote hair growth.

In one aspect, the present invention provides a cosmetic composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

As used herein, the term "camellia japonica " is an evergreen tree belonging to the family Chamophyllaceae, and numerous horticultural varieties have been made and most have been used as ornamental trees or as camellia oil extracted from seeds.

As used herein, the term "pericarp (果皮, pericarp)" refers to all parts of the camellia fruit except for the seeds (seeds).

The term "extract" in the present invention refers to a liquid component obtained by immersing a target substance in various solvents, and then extracting and fractionating it for a certain time at room temperature or a warm state, and removing the solvent from the liquid component. It means the result of one solid content, etc. In addition, in addition to the result, it can be comprehensively interpreted as including all of the diluent of the result, the concentrate thereof, the prepared product thereof, the purified product, and the like.

The camellia peel extract can be obtained by extraction with water or various organic solvents. At this time, the organic solvent used is not particularly limited thereto, as long as an extract having an effect of preventing hair loss or promoting hair growth can be obtained, but preferably water, a polar solvent or a non-polar solvent, and more preferably water, A lower alcohol having 1 to 4 carbon atoms (ethanol, methanol, propanol or butanol, etc.), a mixed solvent thereof, etc. may be used, and ethanol may be used most preferably.

In addition, the method for obtaining the extract is also not particularly limited thereto, as long as it can obtain an extract having an effect of preventing hair loss or promoting hair growth. The extraction method includes cold-chill extraction, ultrasonic extraction, or reflux cooling extraction method, and it is preferable to perform the ultrasonic extraction method.

The ultrasonic extraction is a method of extracting useful components from various natural materials at room temperature by physical force rather than chemical force using a vibration element with a frequency of 20,000 times per second, solvent extraction, vegetable oil extraction, or steam distillation extraction Unlike this, it has the advantage of being able to extract at a high concentration without destroying the active ingredients contained in natural materials at room temperature.

As a specific example, the ultrasonic extraction may be carried out for 24 hours to 72 hours at room temperature using a 60% to 80% ethanol solution of dried camellia peel powder. Preferably, it is carried out for 36 hours to 60 hours at room temperature using a 65% to 75% ethanol solution, and more preferably, it is carried out for 48 hours at room temperature using a 70% ethanol solution. If it exceeds the lower limit of the above numerical value, the active ingredient of the present invention contained in the camellia peel is not sufficiently extracted. will do

The obtained camellia peel extract is not limited thereto, but may be concentrated under reduced pressure using a vacuum concentrator or a vacuum rotary evaporator. In addition, after concentration under reduced pressure, but not limited thereto, it may be dried through freeze drying, reduced pressure drying, vacuum drying, boiling drying, or spray drying.

In the present invention, "hair loss" refers to a state in which there is no hair in a region where hair should normally exist, and specifically refers to the loss of hair (thick and dark hair) of the scalp. The cause of hair loss is not limited, but there are, for example, hereditary androgenic alopecia (baldness), alopecia areata, ringworm of the head due to fungal infection, telogen hair loss, hair growth wall, hair growth disorder disease, etc. may mean hair loss due to lupus, folliculitis solitary, lichen planus pilaris, hair loss due to burns and trauma.

The term "prevention" of the present invention means preventing any thing or phenomenon from occurring, and in the present invention, preventing hair loss means preventing hair loss from occurring.

In the present invention, "hair growth" means that hair grows in the hair follicle. The hair includes hair roots and hair follicles of the head, hair and eyelashes, eyebrows, beards, armpits, pubic hair, and all parts of the body where there are hair roots and hair follicles.

In the present invention, the hair loss prevention or hair growth promoting effect may be achieved by promoting the proliferation of dermal papilla cells, inhibiting the activity of 5-alpha reductase, and activating the signal transduction pathway of Wnt-beta catenin by the extract. .

In one embodiment of the present invention, in order to confirm the hair loss prevention or hair growth promoting effect of the camellia peel extract, the proliferation effect of dermal papilla cells, the inhibitory effect of 5-alpha reductase activity, and the degree of activation of Wnt-beta catenin signaling were analyzed. As a result, it was found that the camellia peel extract promoted the proliferation of dermal papilla cells, excellently inhibited the activity of 5-alpha reductase in a concentration-dependent manner, and effectively activated the Wnt-beta-catenin signaling pathway (FIGS. 3).

As described above, the camellia peel extract provided in the present invention activates the signal transduction pathway of Wnt-beta-catenin to induce proliferation of dermal papilla cells, thereby promoting hair growth, and dihydrotestosterone, which is one of the causes of hair loss. It is possible to prevent hair loss by inhibiting the activity of 5 alpha reductase, which converts it from testosterone. Accordingly, the camellia peel extract of the present invention can be usefully used as an active ingredient in a cosmetic composition, a pharmaceutical composition or a food composition for preventing hair loss and promoting hair growth.

In the present invention, the "cosmetic composition" includes hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, sol gel, emulsion, oil, wax and aerosol. It can be selected from cosmetics for hair consisting of.

The cosmetic composition may include a conventional auxiliary or cosmetically acceptable carrier such as a stabilizer, a solubilizer, a vitamin, a pigment, and a fragrance commonly used in the field of cosmetic compositions.

The cosmetic composition may include a moisturizing agent, an anti-inflammatory agent, an antibacterial agent, an antifungal agent, a vitamin, a sunscreen agent, an antibiotic, a perfume, and a dye, if necessary.

Specifically, the cosmetic composition may be prepared in any conventionally prepared formulation, for example, a solution, emulsion, suspension, paste, cream, lotion, gel, powder, spray, surfactant-containing cleansing, oil, It may be formulated as soap, liquid detergent, bath agent, foundation, makeup base, essence, lotion, foam, pack, softening water, sunscreen cream or sun oil, but is not limited thereto.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, or tracanth may be used.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used.

The carrier may be a non-naturally occurring carrier.

In another aspect, the present invention provides a pharmaceutical composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

The effect of preventing hair loss and promoting hair growth of the camellia fruit skin extract is as described above.

As used herein, the term "pharmaceutical composition" refers to one prepared for the purpose of preventing or treating a disease, and each can be formulated in various forms according to a conventional method and used. For example, it can be formulated in oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and aerosols, and diluents or excipients such as lubricants, wetting agents, flavoring agents, emulsifying agents, suspending agents, preservatives, surfactants, etc. It can be used by formulating it as a parenteral formulation using In addition, it can be formulated and used in the form of external preparations, suppositories, external preparations for skin, and sterile injection solutions. Specifically, the composition of the present invention can be used by a method such as applying or dispersing directly to the hair or scalp. The hair to which the composition of the present invention is applied includes hair roots and follicles of the head, hair and eyelashes and eyebrows, beard, armpits, pubic hair, hair roots throughout the body, and all areas with hair follicles.

In addition, according to each formulation, it may be prepared by further including a carrier known in the art, such as a pharmaceutically acceptable carrier, such as a buffer, an analgesic agent, a solubilizer, an isotonic agent, a stabilizer, a base, and the like. As used herein, the term "pharmaceutically acceptable carrier" may refer to a carrier or diluent that does not inhibit the biological activity and properties of the injected active ingredient without stimulating the organism. The type of carrier usable in the present invention is not particularly limited, and any carrier commonly used in the art and pharmaceutically acceptable may be used. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and the like. These may be used alone or in mixture of two or more. The carrier may be a non-naturally occurring carrier.

The composition of the present invention can be administered in a pharmaceutically effective amount. The pharmaceutically effective amount means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects, and the effective dose level is determined by the patient's health condition, disease type, severity, The activity of the drug, sensitivity to the drug, administration method, administration time, administration route and excretion rate, treatment period, factors including drugs used in combination or concurrently, and other factors well known in the medical field may be determined according to factors. Specifically, it is generally 0.01 mg to 5000 mg per day per 1 kg of body weight of the administered subject, and may be administered in divided doses once or several times a day at regular time intervals according to the judgment of a doctor or pharmacist, but is not limited thereto.

The content of the camellia peel extract included in the composition is not particularly limited thereto, but is 0.00001 to 100% by weight, preferably 0.0001 to 15% by weight, more preferably 0.001 to 15% by weight, even more preferably based on the total weight of the composition. It may be included in an amount of 0.001 to 10% by weight.

In addition, the pharmaceutical composition of the present invention may be used alone or in combination with other pharmaceutically active compounds exhibiting the effect of preventing or improving hair loss-related diseases or in an appropriate group.

The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or may be administered in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. and may be administered single or multiple. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect with a minimum amount without causing side effects, and can be easily determined by those skilled in the art.

In one example, but not limited thereto, in addition to the pharmaceutical composition comprising the camellia peel extract of the present invention as an active ingredient, minoxidil, finasteride, or spironolactone, which are conventional hair loss treatment agents, is selected and additionally administered can do.

In the present invention, the term "administration" means introducing the pharmaceutical composition of the present invention to an individual by any suitable method, and the administration route of the composition of the present invention is oral or parenteral as long as it can reach the target tissue. It can be administered through

The method of administration of the pharmaceutical composition according to the present invention is not particularly limited, and may follow a method commonly used in the art. As a non-limiting example of the administration method, the composition may be administered by oral administration or parenteral administration. Specifically, the composition of the present invention can be used by a method such as applying or dispersing directly to the hair or scalp.

The pharmaceutical composition according to the present invention may be prepared in various dosage forms according to the desired administration method.

The frequency of administration of the composition of the present invention is not particularly limited thereto, but may be administered once a day or administered several times by dividing the dose.

As another aspect, the present invention provides a method for preventing or treating hair loss-related diseases, comprising administering a pharmaceutical composition comprising a camellia peel extract.

As used herein, the term "individual" may mean any animal, including humans, that has or is likely to develop a hair loss-related disease. The animal may be a mammal, such as a cow, a horse, a sheep, a pig, a goat, a camel, an antelope, a dog, a cat, etc. that requires treatment for symptoms similar to those of humans as well as, but is not limited thereto.

Specifically, the prevention or treatment method of the present invention may include administering the composition in a pharmaceutically effective amount to an individual who has or is at risk of developing a hair loss-related disease. The method of administration is the same as described above.

The prevention may refer to any action of inhibiting or delaying the onset of hair loss-related diseases by administering the composition for preventing hair loss or promoting hair growth according to the present invention to an individual.

In addition, the treatment may refer to any act of administering the composition of the present invention to a subject suspected of having a hair loss-related disease so that the symptoms of the hair loss-related disease are improved or beneficial.

The pharmaceutical composition comprising the camellia peel extract is the same as described above.

For administration, the composition of the present invention may include a pharmaceutically acceptable carrier, excipient or diluent in addition to the active ingredients described above. The carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

A suitable dosage of the composition of the present invention varies depending on the condition and weight of the patient, the severity of the disease, the drug form, and time, but may be appropriately selected by those skilled in the art. Specifically, the dosage of the camellia peel extract may be 50 to 500 mg/Kg.

The hair loss-related diseases are alopecia areata (Alopecia Areata), hereditary androgenetic alopecia (Androgenetic Alopecia), telogen Effluvium, trauma, hair growth wall (Trichoti lomania), pressure (Pressure Alopecia), such as genital alopecia, nasal stiffness, It may include, but is not limited to, syphilis (Alopecia syphlltiac), seborrheic alopecia (Alopecia seborrhecia), symptomatic, scarring, congenital alopecia, etc., but is not limited thereto, and may refer to any disease resulting from hair loss from hair follicles.

As another aspect, the present invention provides a food composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

The effect of preventing hair loss and promoting hair growth of the camellia fruit skin extract is as described above.

When the composition of the present invention is used as a food additive, the camellia peel extract may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to the purpose of use (prophylactic, health or therapeutic treatment), and may further include a food pharmaceutically acceptable food supplement additive. Since the composition of the present invention uses an extract derived from a natural product as an active ingredient, there is no problem in terms of stability, so there is no great limitation on the amount of mixing.

The food composition of the present invention may include all foods in a conventional sense, and may be used interchangeably with terms known in the art, such as functional food and health functional food.

The term "functional food" as used in the present invention means a food manufactured and processed using raw materials or ingredients useful for the human body according to Health Functional Food Act No. 6727, and "functionality" refers to the structure of the human body. And it refers to ingestion for the purpose of obtaining useful effects for health purposes such as regulating nutrients for function or physiological action.

In addition, the term "health functional food" as used herein refers to a food manufactured and processed using a specific ingredient as a raw material for the purpose of health supplementation or by extracting, concentrating, refining, or mixing a specific ingredient contained in the food raw material, It refers to food designed and processed to sufficiently exert biological control functions such as biological defense, regulation of biological rhythm, prevention and recovery of disease, etc., by the above ingredients, and the composition for health food is used for prevention of diseases and prevention of diseases. It can perform functions related to recovery, etc.

There is no limitation on the type of food in which the composition of the present invention for preventing hair loss or promoting hair growth can be used. In addition, the composition comprising the camellia peel extract of the present invention as an active ingredient can be prepared by mixing other suitable auxiliary ingredients that may be contained in food and known additives according to the selection of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and There are vitamin complexes and the like, and it can be prepared by adding the extract according to the present invention and its fractions as main components to juice, tea, jelly, juice, and the like.

In addition, foods that can be applied to the present invention include, for example, special nutritional foods (eg, formula milk, infant food, etc.), processed meat products, fish meat products, tofu, jelly, noodles (eg, ramen, noodles, etc.), health supplements , Seasoned foods (eg soy sauce, soybean paste, red pepper paste, mixed soy sauce, etc.), sauces, sweets (eg snacks), dairy products (eg fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi, pickles, etc.) ), beverages (eg, fruit, vegetable beverages, soy milk, fermented beverages, etc.), and natural seasonings (eg, ramen soup, etc.).

When the health functional food composition of the present invention is used in the form of a beverage, it may contain various sweetening agents, flavoring agents, or natural carbohydrates as additional ingredients, as in a conventional beverage. In addition to the above, the health functional food composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin , alcohol, a carbonation agent used in carbonated beverages, and the like. In addition, it may contain the pulp for the production of natural fruit juice, fruit juice beverage and vegetable beverage.

The camellia peel extract, more specifically, the camellia peel ethanol extract of the present invention, is made from natural medicinal plants, so even when used as a cosmetic composition, pharmaceutical composition or food composition, side effects may be less than that of general synthetic compounds, so it is safely included. It can be useful.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Example 1: Preparation of camellia peel extract

After separating the seeds from the camellia fruit, the remaining pericarp was thoroughly washed to remove foreign substances and impurities, dried in the shade at 20° C. to 35° C., and then pulverized so that the particle size was 1 mm or less. Then, 1 kg of the pulverized camellia peel powder was immersed in a 70% ethanol solvent, followed by ultrasonic extraction for 48 hours, and the resulting extract was filtered using filter paper (Advantec, No. 2). The filtrate was concentrated under reduced pressure to prepare a camellia peel extract.

Example 2: In vitro ( in vitro ) from camellia peel extract ( Camellia japonica Measurement of dermal papilla cell proliferation effect of pericarp coat extract)

To find out what effect the camellia peel extract had on the proliferation of dermal papilla cells, the main cells of hair growth, the dermal papilla cell proliferation ability was measured. Specifically, human dermal papilla cells, HFDPC (Hair follicle dermal papilla cell, PromoCell), were cultured in a dermal papilla cell growth medium containing supplementMix and then inoculated into a 24-well plate (1.5 × 10 ⁴ cells/well), 5% concentration. of CO _{2 in an} incubator at 37° C., and incubated for 24 hours. After dissolving the camellia peel extract in DMEM medium containing 1% FBS, it was treated in each cultured well and cultured again for 72 hours. After 72 hours, the cell culture supernatant was removed, washed once with PBS, diluted 10-fold with MTT reagent dissolved in 1 mg/ml, mixed with the medium, and then treated and incubated for 1 hour. After removing the MTT reagent after 1 hour, 200 μl of DMSO was added per well, formazan was dissolved, and the absorbance was measured at 540 nm, as shown in FIG. 1 .

As shown in FIG. 1, the camellia peel extract exhibited about 60% of dermal papilla cell proliferation ability compared to the dermal papilla cell growth medium group, which is a positive control.

Example 3: In Vitro ( in vitro ) in the 5 alpha reductase activity inhibition effect of camellia peel extract

Camellia peel extract was added to the testosterone reaction solution by concentration to measure the activity of 5 alpha reductase. Specifically, after mixing 1 mmol/L DTT (dithiothreitol), 40 mmol/L potassium phosphate having a pH of 6.5, and 100 μmol/L NADPH, 3.5 μmol/L testosterone was added and finally 0.5 ml of A testosterone reaction solution was prepared. After leaving at room temperature for 20 minutes, finasteride was treated as a positive control, and camellia peel extract was treated in the other reaction solution, and rat liver granule was added to the whole reaction solution, followed by reaction at 37° C. for 30 minutes. After 30 minutes of lyophilization, the amount of testosterone remaining was analyzed by HPLC.

Testosterone was analyzed using a Waters 2695 Separation Module, Waters 2996 Photodiode Array Detector through a Phenomenex Luna C18 4.6 × 150 mm, 2.6 μm (reversed phase) column. For the analysis, a sample dissolved in 0.5 ml of methanol was used as a sample solution, and 2.0 mg of a 98% pure Testosterone standard was precisely weighed and 40 ml of a solution dissolved in methanol was used as a testosterone standard stock solution (50 ppm). Precisely weigh 1 ml of this solution, add methanol to dissolve it, and make 10 ml as a standard solution (5 ppm). The test solution and standard solution were analyzed according to the indicator component analysis method to determine the testosterone content in each sample as shown below [Equation 1] was calculated.

[Equation 1]

STD concentration (㎍/㎖) × A Test /A STD × dilution × (100(%) / 1(㎍/㎖)) × (STD purity)

The result value obtained from this was converted based on 100% of the control group, and the result is shown in FIG. 2 .

As shown in FIG. 2 , the camellia peel extract showed excellent 5-alpha reductase inhibitory ability in a concentration-dependent manner compared to the positive control finasteride.

Example 4: In Vitro ( in vitro ) Measurement of Wnt-beta-catenin signaling activation effect of camellia peel extract in

Wnt-betacatenin signaling is a pathway majorly involved in hair follicle formation and regeneration, and TCF (T-cell factor) signaling is activated as a transcription factor. Activation of the Wnt-beta-catenin signaling pathway was measured using the TCF reporter luciferase system.

Human dermal papilla cells were cultured in a dermal papilla cell growth medium containing supplementMix and then inoculated into a 24-well plate (1.5×10 ⁴ cells/well), and cultured at 37° C. in a _{5% concentration CO 2 incubator for 24 hours.} TCF luciferase plasmid (TCF luciferase plasmid) was transfected using Lipofectamine 3000 reagent (Thermo Fisher scientific, USA). The transfected cells were treated with the camellia peel extract and reacted for 24 hours, and the reacted culture medium was reacted with a Gaussia luciferase substrate to measure the expression level of TCF luciferase. As a positive control, lithium chloride, a Wnt receptor agonist, was used. The results are shown in FIG. 3 .

As shown in FIG. 3 , the camellia peel extract induced TCF luciferase expression compared to the untreated group, and showed similar results compared to the positive control, lithium chloride (LiCl). Through this, it was confirmed that the camellia peel extract effectively activated the Wnt-beta-catenin signaling pathway.

Example 5: Safety confirmation test of camellia peel extract on human skin

A skin safety verification experiment was performed to confirm that the camellia peel extract according to the present invention is safe for human skin. To this end, a cumulative skin irritation test was conducted.

A nourishing cream containing 0.1%, 0.5% and 1% of the camellia peel extract of Example 1, respectively, was prepared. Specifically, purified water, triethanolamine and propylene glycol as aqueous phases were heated to 70° C. and dissolved, and then beeswax, liquid paraffin, oily components, emulsifiers and preservatives were heated to 70° C. and dissolved to add and emulsify. After the emulsification was completed, the solution was cooled to 45°C, and 0.1%, 0.5%, and 1% of camellia peel extract were added and dispersed, respectively, and then cooled to 30°C. As the content of the camellia peel extract was increased to 0.1%, 0.5%, and 1%, the content of liquid paraffin was reduced to 9.91%, 9.51%, and 9.01%, respectively, to prepare a nourishing cream.

Using the nutritional cream prepared as described above, 30 healthy adults were subjected to a total of 9 cumulative patches on the upper forearm every other day for 24 hours to determine whether the camellia peel extract stimulates the skin.

The patching method used a pin chamber (Finn chamber, Epitest Ltd, Finland). After 15 μl of each of the above skin external preparations was dripped into the chamber, patching was performed. The degree of the reaction shown on the skin each time was scored using the following [Equation 2], and the results are shown in Table 1 below.

[Equation 2]

Average reactivity = [[(response index × reactivity)/(total number of subjects × highest score (4 points))] × 100] ÷ number of tests (9 times)

In the reactivity, ± is given a score of 1 point, + is given a score of 2, and ++ is given a score of 4, and when the average reactivity is less than 3, it is judged as a safe composition.

test substance Number of subjects who responded average reactivity 1 week 2 weeks 3 weeks Primary Secondary tertiary 4th 5th 6th 7th 8th 9th ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ control
(Squalene) One - - One - - - - - - - - - - - - - - - - - - - - - - - 0.18 camellia
peel extract
(0.1%)
[Test group 1] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 camellia
peel extract
(0.5%) [Test group 2] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 camellia
peel extract
(One%)
[Test group 3] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00

As shown in Table 1, the number of persons corresponding to ±, +, and ++ in Test Groups 1, 2, and 3 was 0, and the average response was also 0.00. As the test result showed an average reactivity of 3 or less, the camellia peel extract according to the present invention was judged to be a safe material for human skin that does not show a distinct cumulative stimulation pattern.

The composition of the present invention may be prepared as a formulation example as follows, but is not limited thereto.

Formulation Example 1: Cosmetic Formulation

Formulation Example 1-1: Softening lotion

As shown in Table 2 below, a softened lotion containing a camellia peel extract extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Camellia peel extract 0.01 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 carboxyvinyl polymer 0.1 ethanol 10.0 triethanolamine 0.1 Preservatives, trace colors, trace fragrances and trace purified water 82.79 sum 100.0

Formulation Example 1-2: Nutrient lotion

As shown in Table 3 below, a nutrient lotion containing camellia peel extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Camellia peel extract 0.01 beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 liquid paraffin 5.0 squalane 5.0 Caprylic/Capric Triglycerides 5.0 glycerin 3.0 butylene glycol 3.0 propylene glycol 3.0 carboxyvinyl polymer 0.1 triethanolamine 0.2 Preservatives, micro pigments, micro fragrances and micro-purified water 69.69 sum 100.0

Formulation Example 1-3: Nourishing Cream

As shown in Table 4 below, a nourishing cream containing camellia peel extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Camellia peel extract 0.01 beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 liquid paraffin 10.0 squalane 5.0 Caprylic/Capric Triglycerides 5.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, micro pigments, micro fragrances and micro-purified water 56.79 sum 100.0

Formulation Example 1-4: Massage Cream

As shown in Table 5 below, a nourishing cream containing camellia peel extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Camellia peel extract 0.01 beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 liquid paraffin 40.0 squalane 5.0 Caprylic/Capric Triglycerides 4.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, micro pigments, micro fragrances and micro-purified water 27.49 sum 100.0

Formulation Example 1-5: Pack

As shown in Table 6 below, a pack containing camellia peel extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Camellia peel extract 0.01 polyvinyl alcohol 13.0 Sodium Carboxymethylcellulose 0.2 allantoin 0.1 ethanol 5.0 nonylphenyl ether 0.3 Preservatives, micro pigments, micro fragrances and micro-purified water 81.39 sum 100.0

Formulation Example 2: Pharmaceutical Formulation

Formulation Example 2-1: Preparation of powder

ingredient Content (g) Camellia peel extract 2 lactose One

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2-2: Preparation of tablets

ingredient Content (mg) Camellia peel extract 100 corn starch 100 lactose 100 Magnesium Stearate 2

After mixing the above ingredients, tablets were prepared by tableting according to a conventional method for manufacturing tablets.

Formulation Example 2-3: Preparation of capsules

ingredient Content (mg) Camellia peel extract 100 corn starch 100 lactose 100 Magnesium Stearate 2

After mixing the above ingredients, the capsules were prepared by filling in gelatin capsules according to a conventional manufacturing method of capsules.

Formulation Example 2-4: Preparation of gel

ingredient weight% Camellia peel extract 1.00 Butylene Glycol 2.00 glycerin 1.00 Disodium EDIT 0.02 PEG-60 Hydrogenated Castor Oil 1.00 Cocamidopropyl Betaine 4.00 Lauryl Glucoside 6.00 Polysorbate 20 1.00 Sodium Benzoate 0.40 caprylic glycol 0.20 sodium lactate 0.50 Hydroxyethyl Cellulose 0.7 Purified water 82.18 sum 100.0

After mixing the above ingredients, a gel containing camellia peel extract as an active ingredient was prepared according to a conventional method.

Formulation Example 2-5: Preparation of ointment

ingredient weight% Camellia peel extract 1.0 diethanolamine 1.5 polyvinylptolidone 5.0 propylene glycol 30.0 Purified water 62.5 sum 100.0

After mixing the above ingredients, an ointment containing camellia peel extract as an active ingredient was prepared according to a conventional method.

Claims (6)

A cosmetic composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.
The cosmetic composition for preventing hair loss or promoting hair growth according to claim 1, wherein the extraction solvent of the camellia peel extract is water, a C1-C4 lower alcohol, or a mixture thereof.
The cosmetic composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition promotes dermal papilla cell proliferation, inhibits the activity of 5-alpha reductase, and activates a signal transduction pathway of Wnt-beta catenin.
According to claim 1, wherein the cosmetic composition is hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, sol gel, emulsion, oil, wax and aerosol. A cosmetic composition for preventing hair loss or promoting hair growth, selected from cosmetics for hair.
A pharmaceutical composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.
A food composition for preventing hair loss or promoting hair growth, comprising a camellia peel extract as an active ingredient.

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