KR20210090770A - Cosmetic composition containing edible flowers extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of edible flowers used as flower tea as an active ingredient. According to the present invention, provided is a cosmetic composition containing a mixed extract of Crocus sativus flower, Tropaeolum majus flower, Cytisus scoparius flower and Hibiscus flower as an active ingredient. The cosmetic composition containing the mixed extract as an active ingredient exhibits excellent skin wrinkle alleviation and skin elasticity improvement effect, skin moisturizing effect, skin regeneration effect and skin irritation alleviation effect.

Description

Cosmetic composition containing edible flower extract for improving skin {Cosmetic composition containing edible flowers extract}

The present invention relates to a cosmetic composition for skin improvement containing a mixed extract of edible flowers, and more specifically, it contains a mixed extract of edible flowers such as saffron flower, nasturtium flower, calendula flower and hibiscus flower used as flower tea as an active ingredient to provide excellent skin It relates to a cosmetic composition having an improving effect.

Common symptoms of skin aging include wrinkle formation, decrease in skin moisture content, and loss of elasticity. In particular, skin wrinkles are one of the aging patterns that are the standard for judging the degree of aging. Skin aging can be broadly divided into two types: intrinsic skin aging that occurs naturally with age, and photoaging that occurs due to external factors such as UV rays. Intrinsic aging includes fine wrinkles, dry skin, and loss of elasticity. Photoaging causes thick and deep wrinkles compared to intrinsic aging, and is accompanied by pigment disease caused by ultraviolet rays. In addition, compared to endogenous aging, the degeneration of elastic fibers is severe and the amount of collagen is severely reduced, resulting in thinner skin and lower skin elasticity. Photoaging is a disease that can be prevented and treated, and it is a field that requires the development of a new concept of treatment technology based on the study of the development mechanism.

The present inventors prepared a mixed extract of edible flowers used as flower tea while searching for natural substances having an effect on skin improvement and studied its activity. Among them, a mixture extracted by mixing saffron flowers, nasturtium flowers, hydrangea flowers and hibiscus flowers The present invention was completed by confirming that the extract exhibits excellent skin improvement effect by its synergistic effect.

(0001) Republic of Korea Patent Publication No. 10-2018-0128774 (2018.12.04) (0002) Republic of Korea Patent Publication No. 10-2010-0031068 (2010.03.19)

An object of the present invention is to provide a cosmetic composition containing a mixed extract of saffron flower, nasturtium flower, peony flower and hibiscus flower as an active ingredient and having excellent skin improvement effect.

According to the present invention in order to achieve the above object, there is provided a cosmetic composition containing a mixed extract of saffron flower, nasturtium flower, marigold flower and hibiscus flower as an active ingredient. In this case, the mixed extract as an active ingredient is contained in an amount of 0.01 to 90% by weight based on the total weight of the cosmetic composition.

The mixed extract is saffron flower, nasturtium flower, calendula flower, and hibiscus flower in a weight ratio of 1-5:1-5:1-5:1-5, more preferably 1-2:1-2:1, respectively. It is extracted by mixing in a weight ratio of ~2:1~2.

The mixed extract is a hot water extract or from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether. a solvent extract with at least one solvent selected.

The cosmetic composition is characterized in that it is for improving skin wrinkles, improving elasticity, moisturizing the skin, regenerating the skin or alleviating skin irritation.

The mixed extract of saffron flower, nasturtium flower, peony flower, and hibiscus flower according to the present invention has excellent skin wrinkle improvement, skin elasticity improvement, skin moisturizing, skin regeneration and skin irritation alleviation effects. The improvement effect is excellent.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition containing, as an active ingredient, a mixed extract of saffron flower, nasturtium flower, hibiscus flower and hibiscus flower, which has been conventionally used as a flower tea.

Saffron (Crocus sativus) is a plant belonging to the iris family and is known as the most expensive spice on earth. Saffron is rich in vitamins and minerals and has antioxidant properties, so it helps to relieve asthma, insomnia, arteriosclerosis, high blood pressure, and memory loss. It is also effective in protecting brain cells, improving inflammation, detoxifying and activating cell functions. is known

Tropaeolum majus is a vine-like annual plant in the genus chrysanthemum, meaning 'tropaion' in Greek, and is said to be derived from the shape of leaves and helmet-like flowers. Orchids are used in flowers, leaves, fruits, and seeds. They contain a large amount of iron and vitamin C, so eating the leaves, flowers and fruits has blood purification and detoxification effects.

Sarothamnus scoparius is used as a garden tree or park tree because of its beautiful flowers and strange leaf shape. In oriental medicine, along with roots and leaves, it is used as medicine, and is mainly used to relieve heat, detoxify, and treat inflammation.

Hibiscus is a natural vitamin called the flower of the gods. It is said to be a flower that resembles a beautiful goddess. It is a compound word that combines the beautiful poems 'hibis' and 'isco' to resemble a flower, meaning 'a flower that supports the gods'. . Hibiscus is rich in vitamin C, which prevents skin aging and helps skin health. It is rich in anthocyanin, an antioxidant component, that makes skin bright and beautiful.

According to one embodiment of the present invention, the mixed extract of saffron flower, nasturtium flower, calendula flower and hibiscus flower as an active ingredient is prepared by the following method.

A mixture is prepared by mixing saffron flowers, nasturtium flowers, calendula flowers, and hibiscus flowers in a dry weight ratio of 1-5:1-5:1-5:1:5, respectively. The mixture is mixed with at least one solvent selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol, preferably water and a carbon number of 1 to 4 A mixed extract is prepared by extraction with a mixed solvent of alcohol.

The edible flower mixture extract prepared in this way has superior wrinkle-related factor expression increase effect (Test Example 1), elasticity-related factor expression increase effect (Test Example 2), and moisturizing factor expression increase compared to each extract by its synergistic effect. Effects (Test Example 3) and skin cell regeneration effect (Test Example 4) were shown. In addition, the cosmetic composition containing it exhibited an excellent skin irritation alleviation effect (Test Example 5).

At this time, when saffron flower, nasturtium flower, marigold flower, and hibiscus flower were mixed and extracted in a weight ratio of 1 to 2:1 to 2:1 to 2:1 to 2, respectively, excellent skin improvement activity was shown.

The mixed extract of saffron flower, nasturtium flower, peony flower and hibiscus flower prepared in this way is contained as an active ingredient in an amount of 0.01 to 90% by weight based on the total weight of the cosmetic composition.

The cosmetic composition containing the edible flower mixed extract of the present invention can be prepared in any conventionally prepared formulation, for example, a lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, conditioner, hair conditioner, hair gel, foundation, lipstick, mascara, makeup base, mask sheet, sun products, soap, and the like.

[Example]

Hereinafter, the present invention will be described in detail through the following examples and test examples, but the present invention is not limited by these examples.

Example 1: Preparation of mixed extracts of saffron flower, nasturtium flower, calendula flower and hibiscus flower

3 g of dried saffron flowers, 3 g of nasturtium flowers, 3 g of hydrangea flowers, and 3 g of hibiscus flowers (dry weight ratio = 1:1:1:1), put 1 kg of 70% aqueous ethanol into a sample and extractor with cooling condenser After extraction by boiling at 100°C for 8 hours, it was filtered using a filter paper (Qualitive Filter Papers NO.2) and obtained as a filtrate.

Examples 2 to 11: Preparation of mixed extracts of saffron flower, nasturtium flower, calendula flower and hibiscus flower

A mixture having a total weight of 12 g was prepared by varying the mixing weight ratio as shown in Table 1 below, and extracted and filtered in the same manner as in Example 1 to prepare a mixed extract.

(weight ratio) Example 2 3 4 5 6 7 8 9 10 11 saffron flower One One One 2 2 2 2 2 2 2 nasturtium flower One One 2 One One One 2 2 One 2 peony flower One 2 One One One 2 One One 2 2 hibiscus flower 2 One One One 2 One One 2 2 One

Comparative Example 1: Preparation of saffron flower extract

12 g of dried saffron flowers were placed in 1 kg of 70% aqueous ethanol, boiled at 100 ° C for 8 hours in an extractor equipped with a cooling condenser, and extracted, filtered using a filter paper (Qualitive Filter Papers NO.2), and then obtained as a filtrate.

Comparative Example 2: Preparation of nasturtium flower extract

12 g of dried chrysanthemum flowers were placed in 1 kg of 70% aqueous ethanol, boiled at 100 ° C for 8 hours in an extractor equipped with a cooling condenser, and extracted, filtered using a filter paper (Qualitive Filter Papers NO.2), and then obtained as a filtrate.

Comparative Example 3: Preparation of extracts from peony flowers

12 g of dried peony flowers were placed in 1 kg of 70% aqueous ethanol, boiled at 100° C. for 8 hours in an extractor equipped with a cooling condenser, and extracted, filtered using a filter paper (Qualitive Filter Papers NO.2), and then obtained as a filtrate.

Comparative Example 4: Preparation of hibiscus flower extract

12 g of dried hibiscus flowers were placed in 1 kg of 70% aqueous ethanol, boiled at 100 ° C for 8 hours in an extractor equipped with a cooling condenser, and extracted, filtered using a filter paper (Qualitive Filter Papers NO.2), and then obtained as a filtrate.

Test Example 1: Confirmation of the effect of increasing expression of wrinkle-related factors

^{Fibroblasts (HDFa) were adjusted to 5×10 6} cells/well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), inoculated in a 6 well plate, and cultured for 24 hours. After incubation, the samples of Examples and Comparative Examples were treated and cultured for 48 hours. RNA was extracted from the cultured cells, and the expression increase rate of collagen was confirmed through RT-PCR (Reverse transcription polymerase chain reaction). The collagen expression increase rate was calculated by the following formula, and the results are shown in Table 2 below. As a positive control, retinol palmitate was used.

Collagen expression rate (%) = (sample collagen expression amount / negative control collagen expression amount) × 100

Sample name Treatment concentration (w/v%) Collagen expression rate (%) Mixed extract (Example 1) 0.5 185.3 Mixed extract (Example 2) 0.5 186.5 Mixed extract (Example 3) 0.5 188.2 Mixed extract (Example 4) 0.5 185.2 Mixed extract (Example 5) 0.5 187.6 Mixed extract (Example 6) 0.5 187.5 Mixed extract (Example 7) 0.5 187.6 Mixed extract (Example 8) 0.5 186.9 Mixed extract (Example 9) 0.5 190.2 Mixed extract (Example 10) 0.5 186.5 Mixed extract (Example 11) 0.5 185.3 Saffron Flower Extract (Comparative Example 1) 0.5 184.2 Orchid flower extract (Comparative Example 2) 0.5 184.5 Peony flower extract (Comparative Example 3) 0.5 183.6 Hibiscus flower extract (Comparative Example 4) 0.5 184.3 retinol palmitate 0.01 188.5

As shown in Table 2, when treated at the same concentration, the mixed extracts of saffron flower, nasturtium flower, marigold flower, and hibiscus flower of Examples showed a higher collagen expression rate than each of the extracts of Comparative Examples 1-4. Through this, it was confirmed that there is a synergistic effect by the mixing. Among them, the mixed extract of Example 9 showed the most excellent effect of increasing collagen expression.

Test Example 2: Effect of increasing expression of elasticity-related factors

^{Fibroblasts (HDFa) were adjusted to 1×10 6} cells/well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), inoculated in a 6 well plate, and cultured for 24 hours. After incubation, the samples were treated and incubated for 48 hours. RNA was extracted from the cultured cells, and the expression increase rate of tropoelastin was confirmed through reverse transcription polymerase chain reaction (RT-PCR). Tropoelastin expression increase rate was calculated by the following formula, the results are shown in Table 3 below. As a positive control, retinol palmitate was used.

Tropoelastin expression rate (%) = (Tropoelastin expression amount in sample / amount of Tropoelastin expression in negative control group) × 100

Sample name Treatment concentration (w/v%) Tropoelastin expression rate (%) Mixed extract (Example 1) 0.5 188.5 Mixed extract (Example 2) 0.5 187.5 Mixed extract (Example 3) 0.5 187.3 Mixed extract (Example 4) 0.5 187.5 Mixed extract (Example 5) 0.5 189.2 Mixed extract (Example 6) 0.5 188.5 Mixed extract (Example 7) 0.5 188.3 Mixed extract (Example 8) 0.5 187.2 Mixed extract (Example 9) 0.5 192.3 Mixed extract (Example 10) 0.5 189.5 Mixed extract (Example 11) 0.5 189.3 Saffron Flower Extract (Comparative Example 1) 0.5 186.2 Orchid flower extract (Comparative Example 2) 0.5 185.3 Peony flower extract (Comparative Example 3) 0.5 184.2 Hibiscus flower extract (Comparative Example 4) 0.5 186.2 retinol palmitate 0.01 184.2

As can be seen in Table 3, when treated at the same concentration, the mixed extracts of saffron flower, nasturtium flower, marigold flower and hibiscus flower of Examples showed a higher tropoelastin expression rate than the extracts of Comparative Examples 1-4. Through this, it was confirmed that there is a synergistic effect by the mixing.

Test Example 3: Effect of increasing expression of moisturizing factors

^{Fibroblasts (HDFa) were adjusted to 1×10 6} cells/well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), inoculated in a 6 well plate, and cultured for 24 hours. After incubation, the samples were treated and incubated for 48 hours. RNA was extracted from the cultured cells and the expression increase rate of hyaluronana synthase-2 (HAS-2) was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The HAS-2 expression increase rate was calculated by the following formula, and the results are shown in Table 4 below. As a positive control, hyaluronic acid was used.

HAS-2 expression rate (%) = (sample HAS-2 expression level / negative control HAS-2 expression level) × 100

Sample name Treatment concentration (w/v%) HAS-2 expression rate (%) Mixed extract (Example 1) 0.5 188.2 Mixed extract (Example 2) 0.5 187.6 Mixed extract (Example 3) 0.5 187.6 Mixed extract (Example 4) 0.5 189.5 Mixed extract (Example 5) 0.5 189.2 Mixed extract (Example 6) 0.5 188.3 Mixed extract (Example 7) 0.5 188.1 Mixed extract (Example 8) 0.5 187.2 Mixed extract (Example 9) 0.5 193.2 Mixed extract (Example 10) 0.5 187.6 Mixed extract (Example 11) 0.5 188.5 Saffron Flower Extract (Comparative Example 1) 0.5 185.3 Orchid flower extract (Comparative Example 2) 0.5 186.4 Peony flower extract (Comparative Example 3) 0.5 183.5 Hibiscus flower extract (Comparative Example 4) 0.5 187.2 hyaluronic acid 0.01 183.8

As can be seen in Table 4, when treated at the same concentration, the mixed extract sample of the Example showed a better HAS-2 expression rate than Comparative Examples 1 to 4. In particular, the mixed extract of Example 9 showed the most excellent moisturizing effect.

Test Example 4: Skin cell regeneration effect

A wound healing assay was performed to confirm the skin cell regeneration effect. ^{Keratinocytes (HEKa) were adjusted to 1×10 6} cells/well using Medium 154 medium supplemented with Human Keratinocyte Growth Supplement (HKGK), inoculated in a 6 well plate, and cultured for 18 hours. After scratching a part of the cells, the samples of the Examples and Comparative Examples were treated and cultured for 8 hours. The cell regeneration rate was calculated by the following formula by measuring the wound gap, and the results are shown in Table 5 below. As a positive control, madecassocide was used.

Cell regeneration rate (%)=100-{(sample cell regeneration rate/negative control cell regeneration rate)×100}

Sample name treatment concentration
(w/v%) Cell regeneration rate (%) Mixed extract (Example 1) 0.5 75.9 Mixed extract (Example 2) 0.5 70.2 Mixed extract (Example 3) 0.5 71.3 Mixed extract (Example 4) 0.5 70.8 Mixed extract (Example 5) 0.5 73.6 Mixed extract (Example 6) 0.5 74.5 Mixed extract (Example 7) 0.5 72.9 Mixed extract (Example 8) 0.5 71.8 Mixed extract (Example 9) 0.5 88.5 Mixed extract (Example 10) 0.5 75.6 Mixed extract (Example 11) 0.5 70.8 Saffron Flower Extract (Comparative Example 1) 0.5 60.2 Orchid flower extract (Comparative Example 2) 0.5 59.5 Peony flower extract (Comparative Example 3) 0.5 58.5 Hibiscus flower extract (Comparative Example 4) 0.5 56.7 Madecassoside 0.01 83.8

As confirmed in Table 5 above, when treated at the same concentration, the samples of Examples 1 to 11, which are the mixed extracts of saffron flower, nasturtium flower, marigold flower, and hibiscus flower of the present invention, exhibited superior cell regeneration rate, indicating that the cell regeneration effect is excellent. Could know.

Example 12: Preparation of lotion containing mixed extract

A lotion containing Example 9, which had excellent effects among the mixed extracts of saffron flower, nasturtium flower, hibiscus flower, and saffron flower prepared in the above Example, was prepared according to a conventional method with the composition and content shown in Table 6 below.

(weight%) Example 12 Control Example 1 Mixed extract (Example 9) 2 - glycerin 8 8 Butylene Glycol 4 4 Caprylic/Capric Triglycerides 10 10 cetearyl glucoside 1.5 1.5 Sorbitan Stearate 0.4 0.4 cetearyl alcohol One One 1,2-Hexanediol 0.9 0.9 incense 0.15 0.15 Purified water To 100 To 100

Test Example 5: Confirmation of skin irritation alleviation effect

After inducing skin irritation through the Tape Stripping Method on the inner part of the lower arm for 10 men and women in their 20s and 30s who do not currently have skin allergy symptoms, the lotion of Example 12 and the lotion of Control Example 1 were applied The degree of sedation of stimulation induced by application was evaluated relative to the degree of sedation. First, skin erythema was induced by taping 30 times with general scotch tape on two inner parts of the lower arm. After erythema was induced, the lotion of Example 12 containing the mixed extract according to the present invention was applied, and the lotion of Control Example 1 which did not contain the mixed extract according to the present invention was applied. The degree of skin irritation was evaluated 3 hours after application.

<Evaluation of skin irritation level (erythema)>

0 (no response) 1 (very slight erythema) 2 (faint or mild erythema)

3 (Clear border but mild erythema) 4 (Clear erythema) 5 (Severe erythema)

Sample name degree of skin irritation left right contrast example One One Example 14 0 0

As can be seen in Table 7, the lotion containing the mixed extract of the present invention had an excellent skin irritation alleviation effect.

Claims (7)

A cosmetic composition comprising a mixed extract of saffron flower, nasturtium flower, peony flower and hibiscus flower as an active ingredient. The method according to claim 1, wherein the mixed extract is extracted by mixing saffron flower, nasturtium flower, calendula flower, and hibiscus flower in a weight ratio of 1-5:1-5:1-5:1-5, respectively. cosmetic composition. The cosmetic composition according to claim 1, wherein the mixed extract is contained in an amount of 0.01 to 90% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin wrinkle improvement and elasticity improvement. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin moisturizing. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin cell regeneration. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin irritation relief.