KR100881143B1 - Composition of skin external application containing green tea flower extract - Google Patents

Abstract

The present invention relates to an external preparation for skin containing green tea flower extract as an active ingredient, and more particularly, has an effect of improving wrinkles and improving elasticity, controlling the micro-inflammatory state of skin caused by ultraviolet rays and preventing toxicity by environmental harmful factors. The present invention relates to a skin external preparation composition containing green tea flower extract having excellent skin efficacy while removing and protecting the skin, reducing pigmentation, and maintaining the inherent odor of green tea flower without side effects.

Green tea flower scent, green tea flower extract, wrinkle improvement, skin elasticity improvement, micro-inflammatory control, detoxification cell damage protection, pigmentation control

Description

Composition for skin external application containing green tea flower extract

The present invention is an external composition for skin containing Camellia sinensis var. Sinenis cv.Shuixian and Maoxie extract as an active ingredient, and more specifically, it is produced by ultraviolet light by containing green tea flower extract containing green tea flower odor component The present invention relates to a composition capable of preventing cellular damage by reducing reactive oxygen species (ROS), reducing inflammation of the skin, delaying aging, inhibiting wrinkle formation and preventing skin elasticity.

The skin is the body's primary barrier, protecting body organs from changes in temperature and humidity, ultraviolet rays, and pollutants. However, internally, as hormones age, the secretion of various hormones that regulate metabolism decreases, and the function and activity of immune cells decreases, resulting in decreased biosynthesis of immune proteins and bioconstituent proteins necessary for living organisms. As a result, physical and chemical irritation and stress caused by the increase of ultraviolet rays, free radicals and free radicals due to environmental pollution such as ozone layer destruction, weakens the normal function of the skin, promotes aging and causes pigmentation. In order to prevent this phenomenon and maintain a healthier and more beautiful skin, by using physiologically active substances obtained from various known animals, plants, microorganisms, etc. in cosmetics, it maintains the unique function of the skin and activates skin cells. Efforts have been made to effectively inhibit aging.

The leaves of Camellia sinensis var. Sinenis cv.Shuixian and Maoxie have been interested for a long time and have used tea extracts and their ingredients importantly. Many are revealed. However, green tea flowers are not known for their useful value and do not help the production of green tea leaves. Recently, green tea flowers have a distinctive fresh scent, and the value as a flavoring plant has attracted attention, and a pharmaceutical composition that reproduces the scent of green tea flowers has begun to be developed (Korean Patent No. 10-0645879: Flavor Composition Reproducing Scent of Green Tea Flower). {Perfume composition for expressing the fragrance of Green tea flower}). However, it is still unknown how the green tea flower fragrance ingredient and green tea flower extract improve skin wrinkles, improve elasticity, and control fine inflammation.

Thus, the present inventors have studied the ingredients to protect the skin from external harmful environment or ultraviolet rays and delay the skin aging, when the fragrance components and extracts of green tea flowers as an active ingredient in the skin external preparation composition by ultraviolet rays By removing the reactive oxygen species (ROS) generated and controlling the micro-inflammation produced by external stimuli, it has been found to protect skin cells, delay skin aging, inhibit wrinkle formation, decrease skin elasticity and reduce pigmentation.

In particular, the composition as epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), deanine (Theanine), camphorol (Kaempferol), green tea catechin extract and green tea extract When the present invention further contains at least one selected from the group consisting of the optimum ratios, it has been found that the above effects can be maximized, and the present invention has been completed.

Accordingly, it is an object of the present invention to provide an external preparation composition for skin that can suppress skin aging and improve overall.

In order to achieve the above object, the present invention provides an external composition for skin containing green tea flower extract as an active ingredient.

In the present invention, the green tea flower extract may be characterized by containing a green tea flower odor component, the green tea flower odor component is 4,8-dimethyl-1,3 (E), 7-nonatriene (E It may be characterized as a green tea flower essential oil containing -4,8-dimethyl-1,3,7-nonatriene) and lilac alcohol (lilac alcohol).

Hereinafter, the present invention will be described in more detail.

The green tea flower extract used in the present invention is a green tea flower extract containing green tea flower essential oil containing green tea flower odor component. At this time, the green tea flower essential oil is an oil component obtained by steam distillation of green tea flower, and compared to other essential oils, 4,8-dimethyl-1,3 (E), 7-nonatriene (E-4,8-dimethyl). -1,3,7-nonatriene) and lilac alcohol (lilac alcohol) due to the essential oil that provides a unique flavor and efficacy. In addition to the two specific components, the green tea flower essential oil includes acetophenone, alpha-methylbenzyl alcohol, 2-phenylethyl alcohol, benzyl alcohol, and the like. Contains common essential oil ingredients common to other essential oils.

The method of obtaining the green tea flower extract is as follows. However, the green tea flower extract including the green tea flower scent in the present invention is not particularly limited in its extraction method for the purpose of using green tea flowers, and may be changed according to necessity and purpose.

100 g of green tea flowers were extracted by refluxing three times with 1 L of hexane or similar organic solvents (ether, petroleum ether, etc.) or 1 L of organic solvents (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.). Thereafter, it is left to deposit at 15 ° C. for one day. Repeating the above process twice to obtain an extract, the extract is filtered and the filtrate is concentrated in a vacuum concentrator to obtain 1.2 g of green tea flower extract. The green tea flower extract including the green tea flower scent obtained by the above method contains various catechins and amino acids extracted from the green tea flower in the unique fragrance component of the green tea flower.

In the present invention, in addition to the green tea flower extract epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), deanine (Theanine), camphorol (Kaempferol), green tea catechin extract And it can provide a skin external preparation composition further containing one or more selected from the group consisting of green tea extract (hereinafter referred to as 'green tea active ingredient'). The external preparation composition for skin according to the present invention may further improve the effect of inhibiting and improving skin aging by further containing the green tea active ingredient.

Epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), deanine (Theanine), and camphorol (Kaempferol) in the present invention are Sigma-Aldrich Korea Co., Ltd. (Sigma- Aldrich Co., Ltd. product was used. In addition, the green tea catechin extract and green tea extract were prepared by mixing green tea samples with distilled water and an organic solvent, stirring at room temperature for 12 hours, and the green tea catechin extract was purified by separating the green tea catechins with a high content of green tea catechin as a column. It was prepared by.

In order to obtain the desired effect in the present invention, the green tea flower extract in the external composition for skin according to the present invention is contained in 0.0001 to 30.0% by weight based on the total weight of the composition. If the content of the component is less than 0.0001% by weight it is difficult to expect to act as an active ingredient, if the content exceeds 30.0% by weight may cause problems in formulation stability.

In addition, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), deanine (Theanine), camphorol (Kaempferol) among the green tea active ingredients contained in the green tea flower extract 0.0001 to 3.0% by weight relative to the total weight of the composition, green tea catechin extract, green tea extract is preferably contained 0.0001 to 15.0% by weight based on the total weight of the composition.

The external preparation composition for skin of the present invention has the effect of improving the condition of the skin by removing the active oxygen species (reactive oxygen species, ROS) generated by ultraviolet light detoxification action.

According to the present invention, a skin external preparation composition for preventing skin aging containing the green tea flower extract, a skin external preparation composition for preventing and improving skin inflammation, a skin external preparation composition for preventing and improving skin wrinkle formation, a skin external preparation composition for improving skin elasticity and skin whitening The external preparation composition for skin can be provided.

The external preparation composition for skin according to the present invention is not particularly limited in the formulation, for example, a cosmetic having a formulation of softening water, nourishing cosmetics, massage cream, nourishing cream, pack, gel, essence or skin adhesive cosmetic, Or in transdermal dosage forms such as lotions, ointments, gels, creams, patches or sprays.

In addition, in each formulation of the external preparation composition for skin, other components than the essential components described above may be selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of the external preparation for skin.

Hereinafter, the present invention will be described in more detail with reference to test examples and examples.

Reference Example 1 Preparation and Analysis of Green Tea Flower Extracts Containing Green Tea Flower Flavoring Ingredients

100 g of green tea flowers were extracted by refluxing three times with 1 L of hexane or similar organic solvents (ether, petroleum ether, etc.) or 1 L of organic solvents (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.). After that, it was allowed to stand at 15 ° C. for one day. The above process was repeated twice to obtain an extract, and the extract was filtered and the filtrate was concentrated in a vacuum concentrator to obtain 1.2 g of green tea flower extract containing green tea flower essential oil containing the scent of green tea flowers.

Analysis Example 1 Analysis of Green Tea Flower Extract Containing Green Tea Flower Flavoring Ingredients

The green tea flower extract obtained in Reference Example 1 was transferred to the inlet of GC-MS, and then desorbed for 2 minutes, followed by GC-MS analysis. GC-MS analysis conditions are as follows.

Analysis condition

. Analyzer: HP 5890 II GC. Detector: HP 5972 MSD

. Column: DB-1 (60m X 0.25mm X 0.25um). Carrier Gas: He

. Injection Temperature: 250 ° C. Detector Temperature: 280 ℃

. Oven Temperature: 70 ° C-220 ° C (3 ° C / min). Ionization Voltage: 70eV

. Desorption time: 2min

As a result, the fragrance components of the green tea flower extract extracted by the method of Reference Example 1 are as shown in Table 1.

Spices people content(%) Acetophenone 5.7590 Alpha-Methylbenzyl Alcohol 13.2000 2-phenylethyl alcohol 5.3000 Benzyl alcohol 9.1000 4,8-Dimethyl-1,3 (E), 7-nonatrien 0.0061 Lilac Alcohol 0.0120 Etc 66.6229 Total content 100.0000

As shown in Table 1, the green tea flower extract of Reference Example 1 is 4,8-dimethyl-1,3 (E), 7-nonatriene (E-4) which is a unique component of the green tea flower aroma as described above. Contains 8-dimethyl-1,3,7-nonatriene and lilac alcohol, acetophenone, alpha-methylbenzyl alcohol, 2-phenylethyl alcohol alcohol), benzyl alcohol, etc., as the main odor component.

Test Example 1 Measurement of Inhibition Effect of Reactive Oxygen Species (ROS) Formation by UV Light

The transformed human keratinocytes were cultured in a 96-hole plate incubator for measurement of fluorescence at a concentration of 2 × 10 ⁴ at 37 ° C. and 5% CO ₂ for 1 day. The green tea flower extract and green tea active ingredient of Reference Example 1 were pretreated for 24 hours as shown in Table 1 below, and EGCG was used as the green tea active ingredient. 100 μl of DCFH-DA (2 ', 7-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc) prepared at 20 μM in HEPES-buffered control salt solution (HCSS) was added and incubated for 20 minutes at 37 ° C and 5% CO _2. Washed with HCSS. After adding 100 µl of HCSS containing the same sample, which was pretreated for 24 hours, the fluorescence intensity of DCF (dichlorofluorescein) oxidized with reactive oxygen species (ROS) was measured with a fluorescent plate reader (Ex = 485 nm, Em = 530 nm). UVB (30 mJ / cm ² ) was treated and the fluorescence intensity immediately after treatment and up to 3 hours was measured with a fluorescence plate reader (Ex = 485 nm, Em = 530 nm). The results are shown in Table 1 using the UV control group as 100.

Green Tea Flower Extract EGCG % ROS generation after 3 hours UV control - - 100 Substance Treatment Group 1 0.1 ppm - 76 Substance Treatment Group 2 1 ppm - 61 Substance Treatment Group 3 0.1 ppm 1 μM 64 Material Treatment Group 4 1 ppm 1 μM 45

From Table 1, it can be seen that the green tea flower extract of the present invention reduces the concentration of reactive oxygen species (ROS) generation by ultraviolet rays and its effect is further enhanced when treated with EGCG, an active green tea ingredient. Therefore, it can be seen that it can effectively defend against oxidative stress and detoxify the cells from harmful environments such as air pollution, yellow dust, and ozone as well as ultraviolet rays.

Test Example 2 Determination of Biosynthesis Inhibition Effect of Type I Collagenase (MMP-1) by Ultraviolet Rays

Human fibroblasts were incubated in a 48-hole flat plate incubator at a concentration of 10 ⁴ , and after 24 hours, irradiated with UV A at 15J / cm ² , the medium containing green tea flower extract and green tea active ingredient of Reference Example 1 as shown in Table 2 below. It was replaced with, green tea active ingredient was used EGCG. The supernatant was harvested on day 2 of culture (harvest) to quantify the amount of type I collagenase produced using ELISA (AP biotech RPN2610) method. The results are shown in Table 2 comparing the UV control group to 100.

Green Tea Flower Extract EGCG type ? Collagenase Biosynthesis (%) UV control - - 100 Material Treatment Group 5 0.1 ppm - 76 Material Treatment Group 6 1 ppm - 63 Material Treatment Group 7 0.1 ppm 1 μM 75 Material Treatment Group 8 1 ppm 1 μM 52

From Table 2, it can be seen that the green tea flower extract of the present invention reduces the biosynthesis of type I collagenase due to ultraviolet rays in a concentration-dependent manner, and the effect is further enhanced when treated with EGCG, an active green tea ingredient. Therefore, it can be seen that collagen generated normally in the skin due to the expression of collagenase can be prevented or suppressed from wrinkles generated.

Experiment 3 Measurement of Biosynthesis Inhibition Effect of Tumor Necrosis Factor Alpha by Ultraviolet Rays

Human keratinocytes were cultured in a 12-hole flat plate incubator at a concentration of 10 ⁵ , and after 24 hours, irradiated with ultraviolet B at 30 mJ / cm ² , the green tea flower extract of the Reference Example 1 and the green tea active ingredient were included as shown in Table 3 below. The medium was replaced with EGCG as the green tea active ingredient. Cells were harvested 6-24 hours after culture (harvest) to quantify the amount of tumor necrosis factor alpha (TNF-α) produced using the ELISA (Pharmingen 555212) method. The results are shown in Table 3 comparing the UV control group to 100.

Green Tea Flower Extract EGCG TNF-α biosynthesis (%) UV control - - 100 Material Treatment Group 9 0.1 ppm - 75 Material Treatment Group 10 1 ppm - 59 Material Treatment Group 11 0.1 ppm 1 μM 67 Material Treatment Group 12 1 ppm 1 μM 50

From Table 3, it can be seen that the green tea flower extract of the present invention reduces the biosynthesis of tumor necrosis factor alpha by ultraviolet rays in a concentration-dependent manner, and when the green tea flower extract is treated with EGCG, the green tea active ingredient, the effect is enhanced. Therefore, it can be seen that it is possible to effectively prevent or improve skin inflammation, skin matrix substance reduction, elasticity reduction, wrinkle formation, and the like, which may occur due to ultraviolet rays.

Test Example 4 Measurement of the Inhibitory Effect of Biosynthesis of Type I Collagenase (MMP-1) by Microinflammatory Factors

Human fibroblasts were cultured in a 48-hole plate incubator at 10 ⁴ concentrations, and after 24 hours, tumor necrosis factor alpha (TNF-α, Tumor necrosis factor-α) was 20ng / ml, and the green tea flower extract of Reference Example 1 The green tea active ingredient was replaced with a medium including as shown in Table 4 below, and camphorol was used as the green tea active ingredient. The supernatant was harvested on day 2 of culture and the amount of type I collagenase produced was quantified using ELISA (AP biotech RPN2610) method. The results are shown in Table 4 comparing the TNF-α control group to 100.

Green Tea Flower Extract Camphorol Type I collagenase biosynthesis (%) UV control - - 100 Material Treatment Group 13 0.1 ppm - 80 Material Treatment Group 14 1 ppm - 61 Material Treatment Group 15 0.1 ppm 1 μM 76 Material Treatment Group 16 1 ppm 1 μM 56

It can be seen from the Table 4 that the green tea flower extract of the present invention reduces the biosynthesis of type I collagenase by TNF-α as a microinflammatory factor in a concentration-dependent manner. It can be seen that the effect is further enhanced when treated with the green tea active ingredient kaempferol. Therefore, it can be seen that it is possible to effectively prevent or improve the reduction of skin matrix material, decrease in elasticity, wrinkle formation, and the like, which are mediated by skin inflammation that may be caused by external harmful stimuli.

Test Example 5 Measurement of Melanin Inhibitory Effect Using Pigment Cells

Reference Example of the Green Tea Flower Extract of the Present Invention Hydrogenone and Green Tea Flower Extract of the Present Invention Cultivated Cells by Concentrating Mel-Ab Cells Derived from C57BL / 6 Mice at a Concentration of 10 ⁵ in a 24-hole Plate Culture 1 was added as a test substance and cultured. At this time, the hydroquinone used is a component that suppresses the formation of melanin pigment, a very strong component of the decolorizing effect, the group treated with the hydroquinone was used as a positive control group. Then, the culture medium was removed, washed with PBS, the cells were dissolved with 1N sodium hydroxide, the absorbance was measured at 400 nm, and the melanin production inhibition rate was calculated according to Equation 1 below, and the results are shown in Table 5. Dooley's way).

Hydroquinone Green Tea Flower Extract Melanin production inhibition rate (%) Hydroquinone control 5 μM - 56.4 Material Treatment Group 17 - 0.1 ppm 21.4 Material Treatment Group 18 - 1 ppm 49.7

As shown in Table 5, the green tea flower extract of the present invention was confirmed to show an excellent melanin production inhibition rate compared to hydroquinone. Through this, it can be seen that the pigmentation of the skin can be suppressed, so that the effect on the skin whitening was confirmed.

Test Example 6 Wrinkle Improvement Effect Measurement

In order to confirm the effect of improving wrinkles in humans by the composition of the present invention, according to the composition ratio of Table 6, the external preparations were prepared in the form of nutrition creams, and Examples 1 to 3 were prepared. The unit of content ratio of the following compounding component is weight%.

Ingredient Example 1 Example 2 Example 3 Comparative Example 1 Purified water To 100 To 100 To 100 To 100 Green Tea Flower Extract (Reference Example 1) 0.1 0.1 0.1 - EGCG - 0.1 - - Camphorol - - 0.1 - Vegetable Cured Oil 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 Glycerol Stearate 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 1.00 1.00 Arakidil Behenyl Alcohol & Arakidil Glucoside 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerolate & Propylene Glycol 1.50 1.50 1.50 1.50 Caprylic / Carric Triglycerides 11.00 11.00 11.00 11.00 Cyclomedicon 6.00 6.00 6.00 6.00 Preservative, incense Quantity Quantity Quantity Quantity Triethanol amine 0.1 0.1 0.1 0.1

In order to confirm the wrinkle improvement effect of Comparative Examples and Examples 1, 2 and 3 of Table 6 was evaluated as follows. Eighty healthy women in their thirties were divided into four groups of 20 people for each of the four groups of Comparative Example 1, Examples 1, 2, and 3, and the nutrition cream was applied to the face once a day for 8 weeks. The wrinkles were measured and image analyzed by measuring the condition of the wrinkles with a visiometer (SV600, Courage + Khazaka electronic GmbH, Germany). The results are shown in Table 7 below. The values in Table 7 below represent the average of each parameter value after 8 weeks of application minus the parameter value before application.

Clinical results after 8 weeks of use R1 R2 R3 R4 R5 Comparative Example 1 0.27 0.26 0.21 0.03 0.03 Example 1 -0.19 -0.18 -0.10 -0.03 -0.03 Example 2 -0.23 -0.20 -0.13 -0.04 -0.03 Example 3 -0.21 -0.21 -0.11 -0.03 -0.04

R1: difference between the highest and lowest of the wrinkle contour

R2: Divide the wrinkle contours by 5 spaces, and then average the R1 values

R3: The highest value among the R1 values divided by five

R4: The mean value of the baseline of the fold contour minus the top and valley values of each angle

R5: Difference value of the baseline of the wrinkle contour line minus each wrinkle outline

As shown in Table 7, the external composition of Examples 1, 2 and 3 was found to have a very good skin wrinkle improvement. Example 1 containing the green tea flower extract of the present invention had an anti-wrinkle effect compared to the group coated with Comparative Example 1, Examples 2 and 3 further comprising the green tea active ingredients EGCG and camphorol (Kaempferol) In the group which applied the external preparation composition, the effect was larger than the group which applied the comparative example 1 or Example 1.

Test Example 7 Measurement of Skin Elasticity Improvement Effect

In order to confirm the effect of improving skin elasticity in humans by the composition of the present invention, the following Examples 1 to 3 and Comparative Example 1 of Test Example 6 were evaluated.

40 healthy women in their 30s were divided into four groups of 10 people for the four groups of Comparative Example 1, Examples 1, 2, and 3, respectively, and the external preparation was twice a day under the conditions of temperature 24-26 ° C and 75% humidity. After application to the face for 12 weeks, skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). The results are shown in Table 8 below. The results in Table 8 are described as ΔR8 (R8 (left) -R8 (right)) values of Cutometer SEM 575, where R8 values represent the nature of skin viscoelasticity.

Experimental product Skin elasticity effect Comparative Example 1 0.10 Example 1 0.43 Example 2 0.45 Example 3 0.44

As shown in Table 8, Example 1 containing the green tea flower extract of the present invention was more skin elasticity than the group to which the comparative example was applied, Example 2 further comprising green tea active ingredients EGCG and camphorol And in the group which applied the external preparation composition of Example 3, the increase in skin elasticity was observed more compared with the group which applied the comparative example 1 or Example 1.

Separately, at the end of the test, the subjects were asked to fill out a questionnaire, which was evaluated mechanically and subjectively. The results are shown in Table 9 below.

Applicator Respondents (Personal) Very good Good usually Inadequate Comparative Example 1 One 3 3 3 Example 1 3 5 2 0 Example 2 3 6 One 0 Example 3 2 7 One 0

As shown in Table 9, it can be confirmed through the survey that the skin elasticity is improved when applying Examples 1 to 3, which is a composition according to the present invention.

Hereinafter, based on the above test example, it will be described in more detail, for example, the formulation of the external preparation composition for skin containing the green tea flower extract containing green tea flower odor according to the present invention, the present invention is limited to these formulation examples It is not.

Formulation Example 1 Flexible Cosmetic (Skin Lotion)

Ingredient weight% Purified water To 100 Green tea flower extract containing odor ingredients, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), theanine, camphorol (Kaempferol), green tea At least one mixture selected from the group consisting of catechin extract and green tea extract 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity

Formulation Example 2 Nutritious Longevity (Milk Lotion)

Ingredient weight% Purified water To 100 Green tea flower extract containing odor ingredients, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), theanine, camphorol (Kaempferol), green tea At least one mixture selected from the group consisting of catechin extract and green tea extract 0.1 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

Formulation Example 3 Nutrition Cream

Ingredient weight% Purified water To 100 Green tea flower extract containing odor ingredients, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), theanine, camphorol (Kaempferol), green tea At least one mixture selected from the group consisting of catechin extract and green tea extract 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / Capric Triglycerides 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

Formulation Example 4 Massage Cream

Ingredient weight% Purified water To 100 Green tea flower extract containing odor ingredients, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), theanine, camphorol (Kaempferol), green tea At least one mixture selected from the group consisting of catechin extract and green tea extract 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

[Formulation Example 5] Pack

Ingredient weight% Purified water To 100 Green tea flower extract containing odor ingredients, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), theanine, camphorol (Kaempferol), green tea At least one mixture selected from the group consisting of catechin extract and green tea extract 0.1 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Fiji-12 nonylphenyl ether 0.3

As described above, the green tea flower extract-containing external composition for skin containing the green tea flower odor component of the present invention has the effect of delaying skin aging by protecting skin cells, improving wrinkles, reducing skin elasticity, inhibiting pigmentation, etc. Excellent, including epigallocategin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), deanine (Theanine), camphorol (Kaempferol), green tea catechin extract and green tea extract It may further contain one or more selected from the group consisting of to enhance the effect of skin cell protection and the like to enhance the effect of skin anti-aging.

Claims (13)

delete delete delete delete delete delete delete delete Cosmetic composition for preventing and improving skin inflammation containing green tea flower extract as an active ingredient. A cosmetic composition for preventing and improving skin wrinkle formation containing green tea flower extract as an active ingredient. Cosmetic composition for improving skin elasticity containing green tea flower extract as an active ingredient. Cosmetic composition for skin whitening containing green tea flower extract as an active ingredient. The composition according to any one of claims 9 to 12, wherein the composition is epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), catechin gallate (CG), theanine, cam Skin cosmetic composition, characterized in that it further comprises one or more selected from the group consisting of ferol (Kaempferol), green tea catechin extract and green tea extract.