KR101458758B1 - Garlic extract with the effect of skin whithening and anti-oxient, manufacturing method htereof and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to garlic extract having skin whitening and anti-oxidant activities, a manufacturing method thereof, and a cosmetic composition comprising the same, and more specifically, to a garlic extract prepared by the optimal extraction condition which can produce the garlic extract having skin whitening and anti-oxidant activities, to a manufacturing method thereof, and a cosmetic composition comprising the same.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a garlic extract having a whitening activity and an antioxidative activity, a process for producing the same, and a cosmetic composition containing the same. [0002] GARLIC EXTRACT WITH SKIN WHITHENING AND ANTI- OXIENT, MANUFACTURING METHOD HEREOF AND COSMETIC COMPOSITION COMPOUND THE SAME [

The present invention relates to a garlic extract having a whitening activity and an antioxidative activity, a process for producing the same, and a cosmetic composition containing the extract. More particularly, the present invention relates to a garlic extract having a whitening activity and an antioxidative activity, A method for producing the same, and a cosmetic composition comprising the garlic extract.

The color of the skin is determined by the pigment of melanin, carotene, hemoglobin, etc. present in the epidermis of the skin. The most important of these is melanin, which is a dark brown pigment. Melanin is produced by a kind of oxidation reaction that takes place in melanomas, the organelles in brown cells. That is, tyrosine, which is a kind of amino acid, is oxidized to dihydroxy phenylalanine by tyrosinase, followed by a series of oxidation steps to form a brown or black polymer. Melanin absorbs ultraviolet rays and protects various cells inside the epidermis from ultraviolet rays. When the synthesis of melanin is abnormally low, skin lesions such as vitiligo occur, and when it is excessive, skin color becomes dark, and stain, freckles .

On the other hand, when the stimulation is repeated by the sun ray, which is a major exogenous cause of skin aging, reactive oxygen species are generated, and active oxygen species activates various cell signal transduction systems, thereby damaging the skin constituting protein, nucleic acid and lipid As the ultraviolet radiation dose increases, the degradation of extracellular connective tissue components including collagen and elastin in the dermis tissue is promoted and the wrinkle of the skin is promoted by the damage of the connective tissue component.

Therefore, in order to suppress the formation of spots such as spots and freckles on the skin and suppress the decrease of elasticity due to aging, it is required to search for a method of inhibiting oxidation in the skin and inhibiting the formation of melanin.

One of these approaches has been to use ascorbic acid, kojic acid, albutin, hydroquinone, glutathione, tyrosinase and the like, which are irritating to the skin There is a limitation in use due to stability of the heat, high unit price, stability of the product when the product is applied, and the like. Therefore, there is a continuing effort to find a substance capable of blocking the synthesis of melanin, which is excellent in the removal of active oxygen which is a basic condition for maintaining skin whitening from natural safe raw materials.

On the other hand, garlic is a crop emerging from Dangun Myth and has long been used as a food material familiar to the Korean people and used for various folk remedies. Garlic has various physiological activities such as antioxidant activity, antimicrobial activity, fatigue recovery activity, blood circulation improving activity, lowering of blood lipid level, anti-inflammatory activity, immunity enhancing activity, hepatic function improving activity and anticancer activity. The concentration improvement, blood circulation improvement and the fatigue recovery effect keep the body metabolism smooth and improve the fatigue of the body, thereby contributing to keeping the skin clean and healthy in the living body. The antimicrobial activity is the proliferation of the bacteria in which the skin follicles are present It has excellent antioxidant activity and inhibits the oxidative action in the skin to prevent deposition of melanin pigment. Thus, it exhibits excellent functionality as a food, but it is also a very excellent raw material as an aromatic material.

Also, it is known that the components such as allysine, allysine, and azone, which are exhibited such functionalities of garlic, are known, and when the allysine of garlic reacts with vitamin B1 to become alitiamin, its activity is further increased. For this reason, various methods for using garlic as a raw material for a cosmetic product are disclosed as patents.

Korean Patent Laid-Open No. 10-2009-0026446 discloses a method for producing garlic by heating garlic and drying garlic in a steamer, drying the garlic in the shade for 10 hours, heating and drying the garlic while gradually lowering the temperature, A method of using garlic oil as a raw material for cream, lotion, lotion, essence, gel, soap and liquid detergent has been disclosed. However, in this method, the process requiring 10 hours or more is repeated 9 times or more. Since garlic oil can be obtained, the process is complicated and the yield is low, which limits the industrialization.

Korean Patent No. 10-0333441 discloses that physicochemical changes of allysine components are induced by aging a citron juice or herb plant after heat treatment to breakage of garlic, and then the garlic liquid obtained by high-pressure concentration is distilled and heat- To obtain garlic oil, from which the skin-whitening soap containing the oily phosphorous and the vitamin B1 component and a method for producing the cosmetic agent are proposed. In this method, several steps are required. In order to carry out high-pressure concentration and distillation treatment, the use of equipment is essential.

Korean Patent Laid-Open No. 10-2006-0108567 discloses a method for producing ally thiamine from a thiamine component of potato and allysine, which is a major component of garlic. The garlic is refluxed three times for 5 hours in 70% ethanol aqueous solution, After cooling, filtering with a filter paper, concentrating under pressure and freeze-drying, dissolving in a solvent to prepare an extract containing allysine and its derivatives, extracting the potato by the same method, dissolving in a solvent and mixing with the garlic extract, A method of manufacturing a cosmetic composition having an effect of whitening, wrinkle improvement, atopic improvement, anti-inflammation, and stimulation relaxation.

In the above-mentioned prior arts, although the main point is to induce the binding between the alliin component of garlic and the vitamin B1 component, the allysine component is very unstable and is highly likely to be lost during various extraction processes.

In a cosmetic composition patent (Korean Patent No. 10-0622100) containing one of the components of garlic, the garlic is extracted according to a supercritical extraction method, allysine is obtained by fractionation, and then, The present invention discloses a method for producing a cosmetic composition that exhibits an effect of improving wrinkles, antibacterial, acne or atopic skin. This method requires a supercritical extraction process, and the yield of the extract from garlic is very low in supercritical extraction, and it is difficult to mass-produce at a time.

Meanwhile, in Korean Patent No. 10-1141343, garlic extract is prepared by using garlic, water, a lower alcohol having 1 to 4 carbon atoms and a mixed solvent thereof, distilled to remove the solvent, concentrated under reduced pressure, and freeze-dried Discloses a method for producing a composition for preventing aging by inhibiting SA-beta-gal expression expressed in fibroblasts or keratinocytes using garlic extract as an active ingredient.

It is known that the garlic ingredients are commonly used in cosmetic composition compositions based on the components of garlic or garlic, because they have the effect of whitening, antibacterial, moisturizing and skin aging, and thus they are widely used as cosmetic compositions. It can be appreciated that the stability is well established. However, in these methods, it takes a long time to prepare the extract, and the process for extraction and the necessity of removing the solvent used for extraction are obstacles to massive industrialization of garlic, which is more efficient, It is required to search for a method of extracting the active ingredient of the extract as a flavoring material.

Accordingly, in order to solve the above problems, the present invention provides a garlic extract having excellent free radical scavenging activity and excellent antioxidative activity and whitening activity, a process for producing the extract, and a cosmetic composition containing the same as an effective ingredient.

According to an aspect of the present invention, there is provided a process for preparing a water-soluble polymer, comprising: preparing a solvent by mixing water and butylene glycol in a weight ratio of 3: 1 to 1: 3; Preparing garlic by grinding; And extracting the pulverized garlic by shaking at 15 to 20 ° C for 15 to 30 hours after immersing the pulverized garlic in the solvent which is 1 to 4 times the weight of the pulverized garlic and then extracting garlic Thereby producing an extract.

In another embodiment, the present invention provides a garlic extract having whitening activity and antioxidative activity extracted from garlic by using a solvent in which water and butylene glycol are mixed. The weight ratio of water to butylene glycol may be, for example, 3: 1 to 1: 3. The solvent may be 1 to 4 times the weight of the garlic. The garlic is ground and immersed in the solvent, Lt; 0 > C for 15-30 hours.

In another embodiment, the present invention provides a cosmetic composition having a whitening activity and an antioxidative activity, comprising the garlic extract prepared by the above-mentioned method as an active ingredient. The garlic extract may be contained in an amount of 1 to 10% by weight based on the whole cosmetic composition. The cosmetic composition may be a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, an oil, a wax, a powder, ≪ / RTI >

According to the present invention, garlic extract having high whitening activity and antioxidative activity can be prepared by controlling the type and ratio of the solvent.

In addition, the garlic extract prepared according to the conditions of the present invention has high whitening activity and antioxidative activity, so that a cosmetic composition having excellent whitening effect and anti-aging effect can be produced.

1 is a graph showing the measurement results of DPPH radical scavenging activity of garlic extract according to the weight ratio of water and butylene glycol.
2 is a graph showing the measurement results of ABTs radical scavenging activity of garlic extract according to the weight ratio of water to butylene glycol.
3 is a graph showing the results of measurement of tyrosinase inhibitory activity of garlic extract according to the weight ratio of water and butylene glycol.
4 is a graph showing the results of measurement of DPPH radical scavenging activity of garlic extract according to the weight ratio of the extraction solvent and garlic.
5 is a graph showing the measurement results of ABTs radical scavenging activity of garlic extract according to the weight ratio of the extraction solvent and garlic.
6 is a graph showing the results of measurement of tyrosinase inhibitory activity of garlic extract according to the weight ratio of the extracting solvent and garlic.
FIG. 7 is a graph showing the result of measurement of tyrosinase inhibitory activity of the cosmetic composition according to the content of garlic extract. FIG.

Hereinafter, the present invention will be described in detail.

The present invention provides a garlic extract which can be used for producing an aromatic product such as a soap, a foam cleansing, a skin, a lotion and the like which has high whitening activity and high antioxidative property, a process for producing the same, and a cosmetic composition containing such garlic extract as an active ingredient. More specifically, the present invention provides an optimum extraction condition for producing a garlic extract having excellent whitening activity and antioxidative activity, and provides various fragrance products using the extract.

First, a solvent for preparing a garlic extract having excellent whitening activity and antioxidative activity will be described.

Garlic is a useful raw material for various cosmetics and has various physiological activities. However, it has been known that water or ethanol is often used for extraction, and the process is complicated and takes a long time. In the present invention, To prepare garlic extract having excellent activity and antioxidant activity, a mixture of water and butylene glycol is used.

In the present invention, water and butylene glycol (BG) are mixed as an extraction solvent so as to be used as a material applicable to various kinds of aromatic products having an antioxidative activity and whitening activity by extracting garlic components, .

Butylene glycol is a widely used solvent for extraction and formulation of cosmetic raw materials, and also helps to dissolve ingredients with low solubility, thus helping to extract the ingredients. In addition, it is a safe ingredient because it is harmless even when applied to the skin for a long time, and it stabilizes volatile substances and suppresses volatilization. When used in cosmetics, it prevents crystallization of poorly soluble components, decreases viscosity, It is a solvent that has the advantage of reducing the use of preservatives due to its growth inhibitory action against Gram-positive bacteria, negative bacteria, fungi and yeast.

Hereinafter, the DPPH radical scavenging activity, the ABTS radical scavenging activity and the tyrosinase inhibiting activity of the garlic extract (sample) vary depending on the amount of water and butylene glycol used as the extraction solvent, and examples thereof will be described . The following examples are provided to aid understanding of the present invention and are not intended to limit the scope of the present invention.

[ Example  1] Production of raw garlic extract (water and Of butylene glycol  When the weight ratio is 3: 1)

Dried natural raw garlic was pulverized, and water (DW) and butylene glycol (BG) were mixed at a ratio of 75 wt%: 25 wt% to prepare a solvent. The solvent (V) Respectively. They were mixed thoroughly and then extracted with shaking for 24 hours in a dark room controlled at 15 to 20 ° C. The extracts were filtered with filter paper and used as experimental samples.

[ Example  2] Preparation of raw garlic extract (water and Of butylene glycol  When the weight ratio is 1: 1)

An extract was prepared in the same manner as in Example 1 except that the mixing ratio of water to butylene glycol was 50 wt%: 50 wt%.

[ Example  3] Production of raw garlic extract (water and Of butylene glycol  When the weight ratio is 1: 3)

The extract was prepared in the same manner as in Example 1 except that the mixing ratio of water and butylene glycol was 25 wt%: 75 wt%.

[ Example  4] Preparation of Extract of Garlic Powder Of butylene glycol  When the weight ratio is 3: 1)

The extract was prepared in the same manner as in Example 1, except that garlic powder was used instead of naturally dried fresh garlic.

[ Example  5] Preparation of Extract of Garlic Powder Of butylene glycol  When the weight ratio is 1: 1)

The extract was prepared in the same manner as in Example 2, except that garlic powder was used instead of naturally dried raw garlic.

[ Example  6] Preparation of Extract of Garlic Powder Of butylene glycol  When the weight ratio is 1: 3)

The extract was prepared in the same manner as in Example 3, except that garlic powder which was ground by freeze-drying instead of naturally dried fresh garlic was used.

[ Comparative Example  1] Preparation of raw garlic extract using water as a solvent

A garlic extract was prepared in the same manner as in Example 1, except that water was used as a solvent instead of the mixed solvent.

[ Comparative Example  2] Butylene glycol  Production of raw garlic extract as a solvent

A garlic extract was prepared in the same manner as in Example 1, except that only butylene glycol was used as a solvent instead of the mixed solvent.

[ Comparative Example  3] Preparation of extract of garlic powder using water as solvent

A garlic extract was prepared in the same manner as in Example 4, except that water was used as a solvent instead of the mixed solvent.

[ Comparative Example  4] Butylene glycol  Preparation of garlic powder extract as a solvent

A garlic extract was prepared in the same manner as in Example 4, except that butylene glycol alone was used as a solvent instead of the mixed solvent.

DPPH radical scavenging activity, ABTS radical scavenging activity and tyrosinase inhibitory activity of the garlic extract prepared according to Examples 1 to 6 and the garlic extract prepared according to Comparative Examples 1 to 4 were measured under the following conditions.

DPPH Radical  Measurement of scavenging activity

The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was shown as the electron donating activity for DPPH. That is, 70 μl of the sample extract and 100 μl of DPPH solution (5 mg / 100 ml of ethanol) were mixed in a 96 well plate, followed by reaction at room temperature for 20 minutes, and the absorbance was measured at 525 nm. The DPPH radical scavenging activity was shown by the absorbance ratio of the sample with and without the additive.

DPPH radical scavenging activity (%) = (1-absorbance of sample added / absorbance of non-additive) x 100

ABTS Radical  Measurement of scavenging activity

The ABTS [2,2-azinobis- (3-ethylbenzo-thiazoline-6-sulphonate)] radical scavenging activity was determined by dissolving 7 mM ABTS in 2.6 mM potassium persulfate solution and then reacting in the dark room for 12-16 hours. , 100 μl of ABTS solution was added to 50 μl of the sample, and the reaction was allowed to proceed at room temperature for 10 minutes. Absorbance was measured at 415 nm. The radical scavenging activity was calculated as the absorbance ratio of the sample to which no sample was added.

ABTS radical scavenging activity (%) = (1-absorbance of sample added / absorbance of non-additive) x 100

Tyrosinase  Measurement of inhibitory activity

Tyrosinase inhibitory activity was obtained by mixing 50 μl of 0.2 M potassium phosphate buffer (pH 6.5), 50 μl of 2 mM L-tyrosine, and 10 μl of tyrosinase (1000 U / ml) in 100 μl of the sample, reacting at 37 ° C for 20 minutes, The absorbance was measured using a Multiplate Reader at 20 nm. Tyrosinase inhibitory activity was calculated by the absorbance ratio of the sample to which no sample was added.

Tyrosinase inhibitory activity (%) = (1-absorbance of sample added / absorbance of non-added sample) × 100

The results of measuring DPPH radical scavenging activity, ABTs radical scavenging activity and tyrosinase inhibitory activity for Examples 1 to 6 and Comparative Examples 1 to 4 are shown in FIGS. 1 to 3, respectively.

1, the radical scavenging activity of DPPH radical scavenging activity was the highest in Example 4, which was extracted with water. In the case of raw garlic extract, the extract of butylene glycol (Example 1, The activity of Example 3 and Comparative Example 2) was high. Comparing Examples 1 to 3, the radical scavenging activity was also increased with the use of a solvent having a high addition ratio of butylene glycol. In the case of using a solvent in which water and butylene glycol were mixed in a ratio of 25:75 wt%, fresh garlic The radical scavenging activity was the best when extracted.

Referring to FIG. 2, the ABTs radical scavenging activity of the fresh garlic extract was 90% or more regardless of the extraction solvent. In other words, both of Examples 1 to 3 and Comparative Examples 1 and 2 exhibited excellent radical scavenging activity. However, in the case of garlic powder, the radical scavenging activity was excellent in Examples 4 to 6 in which water and BG were properly mixed. In Comparative Example 3, which was extracted only with pure water, the radical scavenging activity was somewhat lowered, The radical scavenging activity of Comparative Example 4 was very low.

Referring to FIG. 3, the whitening activity of the sample extract was examined through tyrosinase inhibitory activity. As a result, the tyrosinase inhibitory activity of Comparative Example 3 in which the garlic powder was extracted with water was the most excellent as 62.9% In the case of Comparative Example 2 extracted at 100%, the tyrosinase inhibitory activity was the highest at 61.6%. Next, Example 3, in which raw garlic was extracted by mixing water and butylene glycol at 25:75, was 59.3%, and tyrosinase The inhibitory activity was high. However, all of the three samples (Example 3, Comparative Example 2 and Comparative Example 3) are not statistically significant and can be judged to exhibit the same level of activity.

Determination of solvent type and ratio

The type of the solvent is determined based on the results of the radical scavenging activity and the tyrosinase inhibitory activity.

When the raw garlic was extracted with a sole solvent (Comparative Example 1), the tyrosinase inhibitory activity and the DPPH radical scavenging activity were remarkably low, and when the raw garlic was extracted with a sole solvent of butylene glycol (Comparative Example 2), the DPPH radical scavenging activity was low and the ABTs The radical scavenging activity was the lowest.

In addition, when garlic powder was extracted with water alone (Comparative Example 3), the tyrosinase inhibitory activity and the DPPH radical scavenging activity were excellent, but the ABTs radical scavenging activity was the lowest, and the garlic powder was dissolved in butylene glycol alone When extracted (Comparative Example 4), tyrosinase inhibitory activity was excellent, but DPPH radical scavenging activity and ABTs radical scavenging activity were very poor.

Therefore, it is necessary to select an appropriate solvent for preparing an excellent garlic extract having both antioxidant activity and whitening activity. It is considered that the sample having high antioxidant activity and whitening activity at the same time will prevent the action of the oxide in the skin tissue and contribute to enhance the whitening activity. In this respect, It can be seen that the amount of water and the amount of butylene glycol is 25: 75 (Example 3) is the most appropriate.

Next, a description will be given of how much extraction solvent is added to garlic, which is an extraction solute, in terms of extraction process and yield.

If the amount of solvent is too low depending on the ratio of the extraction solvent to the solute in the preparation of the extract, the yield is low. If the amount of the solvent is too large, the concentration is low and there is a complication and a disadvantage that it is required to be concentrated. It is important to set the conditions that can be used immediately without going through another process.

As described above, in Example 3 of the present invention, since the radical scavenging activity is excellent and the tyrosinase inhibiting activity is high, the extraction solvent is set in accordance with Example 3. That is, the conditions for extracting raw garlic powder with a mixed solvent in which water and butylene glycol are mixed at a weight ratio of 1: 3 are set, and a proper mixing ratio between the raw garlic powder and the extraction mixed solvent is determined.

[ Example  7] Extraction solvent and raw garlic Crushed  When the weight ratio is 1: 1

A solvent mixture of water and butylene glycol in a weight ratio of 1: 3 was added to the raw garlic powder, and the mixture was sufficiently mixed and extracted with shaking for 24 hours in a dark room controlled at 15 to 20 ° C. The extracts were filtered with filter paper and used as experimental samples.

[ Example  8] Extraction solvent and raw garlic Crushed  When the weight ratio is 3: 1

Except that the weight ratio of the mixed solvent to the raw garlic ground product is 3: 1.

[ Example  9] Extraction solvent and raw garlic Crushed  When the weight ratio is 5: 1

Except that the weight ratio of the mixed solvent to the raw garlic ground product is 5: 1.

[ Example  10] Extraction solvent and raw garlic Crushed  When the weight ratio is 7: 1

Except that the weight ratio of the mixed solvent to the raw garlic ground product is 7: 1.

[ Example  11] Extraction solvent and raw garlic Crushed  When the weight ratio is 9: 1

And the weight ratio of the mixed solvent to the raw garlic ground product is 9: 1.

The DPPH radical scavenging activity, ABTs radical scavenging activity and tyrosinase inhibitory activity of the garlic extract prepared according to Examples 7 to 11 were measured and the results were as shown in the graphs of FIGS. 4 to 6, respectively.

Referring to FIG. 4, DPPH radical scavenging activity was measured, and it was confirmed that the activity was the highest in Example 8 in which a solvent three times the amount of garlic was added.

5, when the ABTs radical scavenging activity was measured, the difference of the solvent ratio was smaller than that of DPPH radical scavenging activity. However, when the solvent was 3 times the weight of fresh garlic, the activity tended to be higher Respectively.

6, when tyrosinase inhibitory activity was measured, it was found that garlic and solvent were the most active when extracted with the same amount of solvent, and the activity was higher when extracted with a solvent three times the weight of garlic However, there was no significant difference in activity after increasing the amount of solvent.

2. Determine amount of garlic and solvent

The total amount of the garlic and the solvent is preferably about 1: 1 to about 1: 4, more preferably about 1: 1.3 to 1: 3 in terms of the radical scavenging activity and tyrosinase inhibiting activity , It is judged that conditions for obtaining sufficient yield are obtained by mixing garlic with a solvent and extracting it sufficiently.

On the other hand, while the butylene glycol is a colorless transparent viscous liquid, the extract prepared by the present invention can be obtained as a transparent yellowish-yellowish extract in which the strong fragrance of garlic is volatilized and the garlic flavor hardly occurs during extraction.

Meanwhile, the present invention provides a whitening composition comprising the garlic extract as an active ingredient, wherein the composition is a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, an oil, a wax, a powder, a spray, ≪ / RTI >

Hereinafter, a description will be given of a formulation example of a whitening composition prepared using a garlic extract according to an embodiment of the present invention. Hereinafter, a formulation example according to one embodiment of the present invention will be described, but the scope of the present invention is not limited to the following formulation examples.

First, a description will be given of a formulation example in which a skin composition is prepared using the garlic extract according to the eighth embodiment of the present invention.

<Formulation Example  1>

The skin containing the garlic extract according to Example 8 was prepared by a conventional skin preparation method. The skin composition according to the present invention is shown in Table 1 below.

<Formulation Example  2>

The skin composition was prepared in the same manner as in Formulation Example 1 except that 1.0 wt% of the garlic extract according to Example 8 was used.

<Formulation Example  3>

The skin composition was prepared in the same manner as in Formulation Example 1 except that the amount of garlic extract according to Example 8 was 5.0 wt%.

<Formulation Example  4>

The skin composition was prepared in the same manner as in Formulation Example 1 except that the amount of the garlic extract according to Example 8 was 10.0% by weight.

<Formulation Comparative Example  1>

The skin composition was prepared in the same manner as in Formulation Example 1 except that the garlic extract of the present invention was excluded.

number ingredient weight%
(Formulation Examples 1 to 4) weight%
(Formulation Comparative Example 1) One The garlic extract of Example 8 0.1 - 2 1,3-butylene glycol 5.0 5.0 3 Ice water 5.0 5.0 4 Ethanol (95%) 4.0 4.0 5 Glycerin / glyceryl acrylate / acrylic acid polymer, propylene glycol 3.0 3.0 6 Glycereth-26 3.0 3.0 7 Sodium hyaluronate 3.0 3.0 8 glycerin 1.0 1.0 9 Piece 60
Hydrogenated castor oil 0.5 0.5 10 Methyl paraben 0.2 0.2 11 Beta Glucan 0.2 0.2 12 Clofenine 0.15 0.15 13 Edith Aie 0.05 0.05 14 Soluble collagen 0.02 0.02 15 Acetyl hexapeptide-8 0.02 0.02 16 Hydrolyzed elastin 0.02 0.02 17 Human Oligopeptide-1 0.02 0.02 18 Purified water Balance 74.82

The results of measurement of tyrosinase inhibitory activity of the skin composition prepared according to Formulation Examples 1 to 4 and Comparative Formulation Example 1 are shown in FIG.

7, when 0.1 wt% of the garlic extract according to the present invention was added, the tyrosinase inhibitory activity was similar to that of Comparative Example 1 in which the garlic extract was not added. When the garlic extract was added in an amount of 1.0 wt% or more, Respectively. In particular, the greater the content of the garlic extract according to the present invention, the higher the inhibitory activity, but the garlic smell may be produced.

Thus, the lotion composition and the essence composition were prepared as shown in Tables 2 and 3, with reference to Formulation Example 2, in which the tyrosinase inhibiting activity is high while minimizing the garlic odor. At this time, the tyrosinase inhibitory activity of the lotion composition and the essence composition was measured, and the activity was higher than that of the garlic extract of the present invention.

&Lt; Formulation Example 5 > Lotion composition

number Raw material weight% One The garlic extract of Example 8 1.0 2 1,3-butylene glycol 5.0 3 Ice water 1.0 4 Cetyl hexanoate 4.0 5 Glycerin, glyceryl acrylate / acrylic acid polymer, propylene glycol 3.0 6 Glycereth-26 2.0 7 Cyclomethicone 2.0 8 Dimethicone 2.0 9 Cetearyl Olivate. Sorbitan olivate 2.0 10 Cyclopentasiloxane, dimethicone crosspolymer, laureth-4, laureth-23 2.0 11 Sodium hyaluronate 3.0 12 Beads wax 0.5 13 Propylparaben 0.1 14 Triethanolamine 0.1 15 Carbomer 0.05 16 Hydroxyethylcellulose 0.05 17 Disodium iodide 0.05 18 glycerin 1.0 19 Methylparaben 0.2 20 Beta Glucan 0.2 21 Clofenine 0.2 22 Edith Aie 0.02 23 Soluble collagen 0.01 24 Human Oligopeptide-1 0.01 25 Purified water 70.51

&Lt; Formulation Example 6 >

number Raw material weight% One Example 8. 1.0 2 Cyclomethicone 6.0 3 Propylene glycol 5.0 4 Sodium hyaluronate 4.0 5 glycerin 4.0 6 Sodium chloride 1.0 7 Methylparaben 3.0 8 Purified water 76

The foregoing description is merely illustrative of the present invention,

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention. Accordingly, the embodiments disclosed herein are intended to be illustrative rather than limiting, and the spirit and scope of the present invention is not limited by these embodiments. The scope of protection of the present invention should be construed according to the claims, and all the techniques within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims (7)

Mixing water and butylene glycol in a weight ratio of 3: 1 to 1: 3 to prepare a solvent;
Preparing garlic by grinding; And
Wherein the pulverized garlic is immersed in the solvent which is 1 to 4 times the weight of the pulverized garlic and then extracted by shaking at 15 to 20 ° C for 15 to 30 hours to obtain a garlic extract having a whitening activity and an antioxidant activity Gt; A garlic extract prepared by the method of claim 1. delete delete A cosmetic composition comprising the garlic extract of claim 2 as an active ingredient. 6. The method of claim 5,
Wherein the garlic extract is contained in an amount of 1 to 10% by weight. 6. The method of claim 5,
Wherein the composition is a formulation selected from the group consisting of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, an oil, a wax, a powder, a spray, a soap and a detergent.

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