KR101704504B1 - Cosmetic composition for skin whitening and anti-aging containing styela clava tunic extract - Google Patents

Abstract

More particularly, the present invention relates to a cosmetic composition comprising a hederhess skin extract as an active ingredient, and more particularly, to a functional cosmetic product of high added value utilizing a healdock skin, which is a waste resource, , Anti-aging, anti-wrinkle and antioxidant activity, and is effective for skin whitening, skin aging prevention or wrinkle improvement.

Description

[0001] The present invention relates to a cosmetic composition for skin whitening and anti-aging containing styela clava tunic extract,

The present invention relates to a cosmetic composition having a high-value-added functional beauty product by using a hederock shell, which is a waste resource, as an active ingredient, and has an effect of skin whitening, skin aging prevention or wrinkle improvement.

The human skin undergoes changes due to various internal and external factors as it gets older. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction As the amount of ultraviolet rays reaching the surface of the sunlight increases and the environmental pollution is further intensified, free radicals and active noxious oxygen are increased, thereby decreasing the thickness of the skin, increasing the wrinkles and decreasing the elasticity Skin color is not good, skin troubles occur frequently, and spots, freckles and black blots also increase.

As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid, and glycoprotein, which constitute the skin, are changed or decreased, and oxidative stress due to free radicals and active noxious oxygen is experienced. In addition, when the skin ages or the skin is exposed to ultraviolet rays, most of the cells constituting the skin are treated with Cox-2, an enzyme that produces a proinflammatory cytokine known to cause inflammation , and cyclooxygenase), and biosynthesis of matrix metalloproteinase (MMP), an enzyme that degrades skin tissue, is increased by these inflammatory factors, and nitric oxide (NO) by iNOS (inducible nitric oxide synthase) ) Production is known to increase. In other words, the decrease of cell activity due to natural endogenous aging and the decrease of biosynthesis of substrate materials due to microinflammation, the increase of stress caused by various harmful environments, and the increase of active oxygen species by sunlight The degradation and denaturation of the substrate material is accelerated, the skin substrate is destroyed and thinned, and various symptoms of skin aging appear. Therefore, it is a reality that many researches have been conducted on active ingredients that can prevent and improve such aging phenomena.

On the other hand, various factors are involved in determination of human skin color. Among them, activity of melanocyte which makes melanin pigment, distribution of blood vessels, thickness of skin, presence of pigment inside and outside of human body such as carotinoid and bilirubin The factors of importance are.

Especially, the most important factor is melanin, a melanin pigment produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside of the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results. In addition, as the number of people enjoying outdoor activities increased due to the increase in the leisure population, the demand for preventing melanin pigmentation due to ultraviolet rays increased.

In response to such demands, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or thyrocinase inhibiting activity have been conventionally used in cosmetics or pharmaceuticals, The use thereof is limited due to insufficient whitening effect, safety problem to skin, formulation and stability of formulation in cosmetics, and the like.

On the other hand, Ascidian ( Styela clava ) belongs to the rhizomes of the chrysanthemum, and is called differently in different countries such as sea squirt, sea grape, tunicate and so on. Middude is a marine creature well known as a food with animal and plant characteristics. The skin of the skin is covered with a rigid cellulosic form composed of a glycoprotein called proteoglycans. Unlike ordinary marine animals, it has a beta-1,4 structure similar to that of dense vegetable cellulose. Midduk is not currently developed as a special functional material other than being used as a food, and the healdock shell is treated as a whole waste, and is left on the beach, causing environmental pollution problems.

The studies on the midduk include sterol content, changes in nutritional composition according to the season, examples of glycosaminoglycan extracted from the skin, and hemolytic antimicrobial peptides, and chondroitin sulfate extracted from the off- There is a research on the possibility of functional cosmetics using the antioxidant activity, the basic anticancer activity and the cytotoxic effect on the cancer cell line from the thyroid.

However, research and patents related to cosmetic products utilizing the discarded waste, Midduk skin, have not been reported.

Korean Patent Laid-Open No. 10-2011-0090443 Korean Patent Laid-Open No. 10-2008-0018590

In order to solve the problems of the prior art as described above, it is an object of the present invention to provide a cosmetic composition which can produce a high value-added functional beauty product by utilizing a hederock shell, which is a waste resource.

Another object of the present invention is to provide a cosmetic composition having whitening, moisturizing, anti-aging, anti-wrinkle and antioxidative activity without skin irritation and having skin whitening, skin aging prevention or wrinkle-reducing effect.

In order to accomplish the above object, the present invention provides a cosmetic composition for skin whitening comprising an extract of Cinchona bark as an active ingredient.

The extract may be extracted with a solvent such as water, ethanol, methanol, acetone, ethyl acetate, chloroform, n-butanol or n-hexane.

The extract can be extracted by hot water extraction, high pressure hot water extraction, ethanol extraction, methanol extraction, or organic solvent extraction.

In particular, it is preferable that the heder-shell bark extract is contained in an amount of 1 to 50% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may be in the form of a lotion, nutritional lotion, nutritional cream, massage cream, nutritional essence, massage pack or emulsion type foundation.

In addition, the present invention provides a cosmetic composition for preventing skin aging comprising an extract of Rhododendron bark as an active ingredient.

In addition, the present invention provides a cosmetic composition for wrinkle improvement comprising an extract of Chidyuk shell as an active ingredient.

According to the present invention, high value-added functional beauty products can be manufactured by utilizing the Midduk skin, which is a waste resource, and whitening, moisturizing, anti-aging, anti-wrinkle and antioxidant activity can be obtained without skin irritation, Or a cosmetic composition having an excellent efficacy for improving wrinkles.

FIG. 1 is a graph showing tyrosinase inhibitory activity of the hederfly skin extracts prepared according to one embodiment of the present invention.
FIG. 2 is a graph showing the hyaluronidase inhibitory activity of the hederock skin extracts prepared according to one embodiment of the present invention.
FIG. 3 is a graph showing collagenase inhibitory activity of the hederfly skin extracts prepared according to an embodiment of the present invention.
FIG. 4 is a graph showing elastase inhibitory activity of the hederfly skin extracts prepared according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention analyzed flavonoids which are presently discarded as a result of the analysis. As a result, flavonoids and phenol compounds exist in the flavonoid shell, and these flavonoids have functional cosmetic properties such as whitening, moisturizing, anti-aging, anti- It has been confirmed that a high value-added functional beauty product can be manufactured by utilizing the hederock shell, which is a waste resource, and thus the present invention has been completed.

Such a cosmetic composition of the present invention contains a hederfly skin extract as an active ingredient and has skin whitening, anti-aging or wrinkle-reducing effects.

The method for producing the hederfly skin extract is not particularly limited and can be obtained by a conventional method known in the art.

Specifically, the hederock shell is cleaned by hand with various foreign substances such as styrofoam pieces and seaweed contaminated on the outside, and is sufficiently washed with tap water to remove the salt. The washed hemi-shell bark is dried in the shade and dried again in a dry oven at 50 to 80 ° C, preferably 60 ° C until it becomes constant. The dried heald shell is crushed into pieces having a length of 1 to 2 cm using a conventional mixer, and then crushed to a size of 1 to 2 mm using a conventional blender.

The crushed hemi-shell bark can be extracted with water or an organic solvent, followed by concentration and drying to obtain an extract.

As the solvent, water, ethanol, methanol, acetone, ethyl acetate, chloroform, n-butanol, n-hexane or a mixed solvent of these organic solvents and water may be used. Preferably, water, ethanol or acetone is used as the solvent.

In addition, conventional methods can be used for extracting the hederock bark extract using water or an organic solvent. For example, hot water extraction, high-pressure hot water extraction, ethanol extraction, methanol extraction, organic solvent extraction Can be performed. In the case of the extraction of hot water, the extraction is carried out at 80 to 100 ° C, preferably at 100 ° C for 1 to 24 hours, at 100 to 130 ° C for high-pressure hot water extraction, preferably at 121 ° C for 1 to 24 hours, It is preferably carried out at 60 to 90 ° C, preferably at 80 ° C for 1 to 24 hours, at the organic solvent extraction at 1 to 50 ° C, preferably at 4 ° C or 30 ° C for 1 to 3 days. In the above extraction methods, when the extraction temperature is outside the extraction temperature and the extraction time, the extraction efficiency may be lowered or the components may be changed. Therefore, the extraction is preferably performed within the temperature and time range described above.

After the extract is obtained using water or an organic solvent as described above, a liquid material can be obtained by freezing, heating and filtering at room temperature by a conventional method known in the art, or a liquid material can be obtained by further evaporating, spray drying or freeze- You may.

The Thicker shell extract contained as an active ingredient of the present invention has an activity of inhibiting tyrosinase, hyaluronic acid inhibiting activity, collagenase inhibiting activity and elastase inhibiting activity, thereby providing excellent skin whitening, moisturizing, anti-aging and anti- .

The cosmetic composition of the present invention comprising the above extract of Chrysanthemum hull extract as an active ingredient may be a composition for preventing skin whitening or skin aging. It is preferable that the heder bark extract is contained in an amount of 1 to 50% by weight, more preferably 1 to 20% by weight based on the total weight of the cosmetic composition. When the content is less than 1% by weight, the effect of skin whitening or skin aging prevention may be insignificant. When the content is more than 50% by weight, the effect on the usage amount may be insignificant.

As described above, the cosmetic composition of the present invention comprising the extract of Chrysanthemum bark extract as an active ingredient can be used as a conventional auxiliary agent such as antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances and the like which are commonly used in cosmetic compositions, May be further included. For example, the cosmetic composition may further contain an auxiliary component such as glycerin, butylene glycol, polyoxyethylene hardened castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, allantoin and the like .

Since the cosmetic composition of the present invention is basically applied to the skin, it can be prepared into any formulation conventionally produced by referring to the cosmetic composition of the related art. For example, they may be formulated as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, But is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritive cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a mask pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, the carrier component may include an animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, have.

When the formulation of the present invention is a powder or a spray, the carrier component may include lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like. Particularly in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane, dimethyl ether, and the like.

When the formulation of the present invention is a solution or an emulsion, the carrier component may include a solvent, a solubilizing agent, and an emulsifying agent. Specific examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid esters of sorbitan, and the like.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol, propylene glycol or the like as a carrier component; Suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, trachant, and the like.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like.

Particularly, the cosmetic composition containing the extract extract of the present invention as an active ingredient is preferably a mask pack.

The mask pack containing the extract of Chrysanthemum morifolium may be prepared by a conventional method. For example, the extract of Chrysanthemum bark extract, Galactomyces fermentation filtrate, purified water, Carbopol # 940 (2%), , 3-butylene glycol, NATRASOL 250HHR, propylene glycol, allantoin, DL-Panthenol, glycerin, HC-60, PHENONIP, MP, PF (W80402), triethanolamine, polygamma glutamic acid -BG, 1%), HYAL-JOINT (1%), SKIN SILK OH4, aloe extract, GPC, ceramide, BSASM and silk protein.

First, purified water, carbomer, and butylene glycol were mixed and dissolved homogeneously while stirring at a speed of 1,500 rpm for 60 minutes. The aquatic raw materials are then dissolved and the fragrance and preservative raw materials are dissolved while warming to 70-75 占 폚. The mixture is emulsified and mixed at a rate of 2,000 to 3,000 rpm for 30 minutes while being added to the perfume and preservative mixture raw material mixture, and then mixed at a rate of 1,500 to 2,000 rpm for 20 minutes to prepare a mask stock solution. To the above stock solution, the extract of Chrysanthemum bark extract extracted as described above is added in an amount of 1 to 50% by weight based on the total weight of the undiluted solution, thoroughly mixed for 15 minutes, and filtered through 100 mesh to prepare a crude extract of the hederock bark extract. Next, the mask sheet used for a conventional mask pack is filled with the crude solution of the mask for extractor mask, and then mask-bonded using a mask sealer to produce a mask pack.

The present invention is based on the analysis of the components and the whitening, moisturizing, anti-aging, anti-wrinkle and antioxidant activities of the extracts used in the manufacture of the extract of Chrysanthemum morifolium bark, Can be used as a cosmetic composition for skin whitening, skin aging prevention or wrinkle improvement.

Hereinafter, the present invention will be described in more detail with reference to examples. These embodiments are for purposes of illustration only and are not intended to limit the scope of protection of the present invention.

EXAMPLES Example 1: Preparation of the extract

The heder shells obtained from Miedderock were washed as follows. Styrofoam pieces and foreign matter such as seaweeds were cleanly removed by hand and washed thoroughly with tap water to remove salt. The washed Rhododendron husk was air dried in the shade and then dried in a dry oven maintained at 60 DEG C until it became constant. The dried heald shells were crushed into pieces of 1 to 2 cm in length using a domestic mixer, and then crushed to a length of 1 to 2 mm using an experimental blender.

100 g of the crushed hederhess shell was added to 1 L of water, ethanol, methanol, acetone, ethyl acetate, chloroform, n-butanol and n-hexane having different polarities and extracted. At this time, ethanol extraction and methanol extraction are performed at 80 ° C. for 1 to 5 hours, and organic solvent extraction is performed at 4 ° C. and 30 ° C. for 1 to 5 hours at 100 ° C. for 1 to 5 hours, 121 ° C. for 1 to 5 hours for high- , And then extracted three times for 1 to 3 days. After completion of the extraction, each solvent was concentrated by rotary vacuum, concentrated by centrifugal vacuum, and then lyophilized to measure the weight of the solid obtained. The extraction yield was calculated by the following formula (1), and the results are shown in Table 1 below.

[Equation 1]

As shown in Table 1, the extraction yields of the respective solvents showed the highest extraction yields at 5 hours after hot water and high pressure hot water extraction at 100 ° C and 121 ° C, respectively. Ethanol extraction or methanol extraction at 80 ℃ showed the highest extraction yield at 5 hours and there was no significant difference from the extraction at 3 hours. When the extract was extracted with other organic solvents, the yield was slightly higher at 30 ℃ than 4 ℃ and there was no significant difference between extraction times.

Example 2. Determination of flavonoid and phenol compound content of the extract

The content of flavonoids and phenolic compounds contained in each of the extracts of Chrysanthemum morifolium peptides extracted in Example 1 was measured and the results are shown in Table 2 below. Catechin and gallic acid were used as standards for the determination of flavonoids and phenolic compounds, respectively.

division Flavonoid
(Catechin g / g) Phenol compound
(Gallic acid g / g) Hot water extract 0.019 0.0466 High pressure hot water extract 0.033 0.0323 Ethanol extract 0.0156 0.0375 Methanol extract 0.0037 0.0218 Acetone extract 0.0172 0.0243 Ethyl acetate extract 0.0063 0.0261 Chloroform extract 0.0230 0.0247 n-butanol extract 0.0191 0.0222 n-hexane extract 0.0089 0.0218

As shown in the above Table 2, all of the hederhook peel extracts contained 0.0019-0.023 g / g of flavonoids, and the contents were in the order of chloroform, n-butanol, acetone and ethanol extracts. On the other hand, phenolic compounds contained 0.0218-0.0466g / g of each extract, and ethanol, high - pressure hot water, ethyl acetate and chloroform showed the highest contents in that order.

Example 3: Measurement of tyrosinase inhibition activity of the extract of Chrysoperomyces bark

Tyrosinase is an enzyme that synthesizes melanin from L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) in melanocytes in melanoma. Therefore, in this example, the degree of inhibition of tyrosinase activity was examined to confirm whitening activity. To screen for the whitening activity of each of the extracts extracted from Example 1, each extract was concentrated by rotary evaporation, concentrated by centrifugation in vacuo, and lyophilized to obtain a solid. Distilled water or dimethyl sulfoxide (DMSO) / mL to measure tyrosinase inhibitory activity, and the results are shown in Table 3 and FIG.

division Tyrosinase inhibitory activity (%) Hot water extract 54.2 High pressure hot water extract 63.2 Ethanol extract 100 Methanol extract 100 Acetone extract 100 Ethyl acetate extract 100 Chloroform extract 79.2 n-butanol extract 100 n-hexane extract 50.9

As shown in Table 3, the extracts of ethanol, methanol, acetone, ethyl acetate, and n-butanol showed a 100% inhibition of tyrosinase activity at a concentration of 10 mg / mL, and the other extracts showed 50.9 ~ 79.2% Lt; / RTI > As shown in FIG. 1, all of the extracts showed tyrosinase inhibitory activity in a concentration-dependent manner as a result of measuring tyrosinase inhibitory activity according to the concentration of each of the Midduk peel extracts. From these results, it was found that ethanol, methanol, acetone, ethyl acetate, n-butanol hydroperoxide extract had a high inhibitory activity against tyrosinase at a relatively low concentration, while other extracts showed effective inhibitory activity at a high concentration.

Example 4. Measurement of hyaluronidase inhibition activity of the extract

Hyaluronic acid is a mucopolysaccharide with β-1,4-linked glucuronic acid and N-acetylglucosamine, which is involved in moisturizing the skin. Therefore, it is basically used in cosmetics and pharmaceutical preparations. Hyaluronidase is an enzyme that cleaves glucuronic acid of hyaluronic acid and β-1,4 bond of N-acetylglucosamine. Thus, inhibition of hyaluronidase is important in maintaining skin hyaluronic acid levels and skin moisturization. Therefore, in this Example, the hyaluronidase inhibitory activity was examined to confirm the moisturizing activity. To screen for the moisturizing activity of each of the extracts extracted from Example 1, each extract was concentrated by rotary vacuum, concentrated by centrifugation in vacuo, and lyophilized to obtain a solid. Distilled water or dimethyl sulfoxide (DMSO) / mL to measure hyaluronidase inhibitory activity, and the results are shown in Table 4 and FIG. 2 below.

division Hyaluronidase inhibitory activity (%) Hot water extract 4.7 High pressure hot water extract 38.7 Ethanol extract 9.4 Methanol extract 82.1 Acetone extract 100 Ethyl acetate extract 84.9 Chloroform extract 51.7 n-butanol extract 65.5 n-hexane extract 91.1

As shown in Table 4, the acetone heder bark extract inhibited hyaluronidase activity at a concentration of 10 mg / mL by 100%, and methanol, ethyl acetate, and n-hexane showed a relatively high inhibitory activity of 82.7 to 91.1% Respectively. On the other hand, hydrothermal and ethanol extracts showed low inhibitory activity of 4.7% and 9.1%. As a result of measuring the hyaluronidase inhibitory activity according to the concentration of each heder's skin extract, it was confirmed that all of the extracts showed hyaluronidase inhibitory activity in a concentration-dependent manner. From these results, it was found that chloroform, n-butanol, n-hexane and ethyl acetate hederhook extract showed high hyaluronidase inhibitory activity at a relatively low concentration, while other extracts showed effective inhibitory activity at high concentrations.

Example 5: Measurement of collagenase inhibition activity of the extract of Chidyuk shell extract

 Representative symptoms of aged skin are the appearance of fine lines and wrinkles. This is due to a decrease in collagen, the main protein of the skin dermis tissue collagen. Collagen accounts for more than 90% of the dermis and protects the skin from external stimuli because it gives skin tension and strength. In other words, collagen reduction is closely related to skin aging. Synthesis and degradation of collagen in vivo are appropriately controlled, but the synthesis is reduced as aging proceeds, and the expression of collagenase degrading collagen is promoted by ultraviolet irradiation. Therefore, skin aging can be prevented by inhibiting the activity of collagenase. In this example, anti-aging activity was confirmed by examining collagenase inhibitory activity. To screen for the anti-aging activity of each of the extracts extracted from Example 1, each extract was concentrated by rotary vacuum, concentrated by centrifugation in vacuo, and lyophilized to obtain a solid. Distilled water or dimethyl sulfoxide (DMSO) Mg / mL, and the collagenase inhibitory activity was measured. The results are shown in Table 5 and Fig. 3 below.

division Collagenase inhibitory activity (%) Hot water extract 69.6 High pressure hot water extract 25.0 Ethanol extract 44.5 Methanol extract 50.8 Acetone extract 25.5 Ethyl acetate extract 54.6 Chloroform extract 24.1 n-butanol extract 59.7 n-hexane extract 53.4

As shown in Table 5, the extracts of high pressure hot water, acetone, and chloroform extract showed 14.1 ~ 15.5% collagenase inhibitory activity, and the other extracts also exhibited comparatively high collagenase inhibitory activity of 44.5 ~ 69.6% . As shown in FIG. 3, it was confirmed that all the extracts showed collagenase inhibitory activity in a concentration-dependent manner, as a result of measuring the collagenase inhibitory activity according to the concentration of each of the extracts of Midduk peel. From these results, it was confirmed that the hydrothermal and ethanol extracts showed high collagenase inhibitory activity at a low concentration, while other extracts showed effective inhibitory activity at a high concentration.

Example 6. Measurement of elastase inhibition activity of the extract of Chidoruk shell extract

Elastin is an elastic fiber component of connective tissue or tendons, and its content decreases in the skin as it ages. Elastase is an enzyme that degrades elastin. It is also involved in inflammation. It breaks down almost all proteins including connective tissue support such as elastin and collagen, and structural proteins, to form sagging skin. Thus, inhibition of the elastase activity can prevent the occurrence of skin wrinkles. Therefore, in this Example, the anti-wrinkle activity was confirmed by examining the elastase inhibitory activity. To screen for anti-wrinkle activity of each of the extract extracts of Example 1, the extracts were concentrated by rotary vacuum, concentrated by centrifugation in vacuo, and lyophilized to obtain solid components. Distilled water or dimethyl sulfoxide (DMSO) Mg / mL to measure the elastase inhibitory activity, and the results are shown in Table 6 and FIG.

division Elastase inhibitory activity (%) Hot water extract 1.7 High pressure hot water extract 79.6 Ethanol extract 100 Methanol extract 5.0 Acetone extract 79.4 Ethyl acetate extract 86.2 Chloroform extract 46.6 n-butanol extract 52.0 n-hexane extract 8.4

As shown in the above Table 6, the ethanol extract of the extract from the ethanol showed 100% elastase inhibitory activity at a concentration of 0 mg / mL. On the other hand, hydrothermal, methanol and n - hexane extracts showed low inhibitory activity as 1.7 ~ 8.4%, and the other extracts showed relatively high inhibitory activity. As shown in FIG. 4, all of the extracts showed elastase inhibitory activity in a concentration-dependent manner. From these results, it was confirmed that the extract of high pressure hydrothermal, ethanol and acetone heder bark exhibited a high level of inhibition of elastase at low concentration, while the inhibitory activity of other extracts was effective at high concentration.

Example 7. Measurement of minimum inhibitory concentration of extract

Based on the results of Examples 3 to 6, the minimum inhibitory concentration (IC ₅₀ ) of each heder's skin extract was calculated according to Equation 2 using the Sigmaplot program, and the results are shown in Table 7 below .

&Quot; (2) "

In the above formula (2), x represents the logarithmic value of the extract concentration, max represents 100% inhibition, and min represents 0% inhibition.

Example 7. Antioxidant activity measurement of extract

Oxidative stress generates diverse reactive oxygen species through biochemical reactions and causes harmful symptoms such as skin aging. In order to screen for the antioxidative activity of each of the extracts extracted from Example 1, the extracts were concentrated under reduced pressure by centrifugation, concentrated by centrifugation in vacuo, and lyophilized to obtain solid components. Distilled water or dimethyl sulfoxide (DMSO ), The reducing power, the ABTS radical scavenging activity, the DPPH radical scavenging activity, the metal chelating activity, the NO scavenging ability and the superoxide dismutase (SOD) -like activity were measured and the results are shown in Table 8 . The units in Table 8 are%.

extract Reducing power ABTS radical scavenging ability DPPH radical scavenging ability Metal chelating activity NO scavenging ability SOD similar
activation Heat number 97.8 28.4 82.5 30.9 75.4 20.6 High-pressure hot water 100 32.7 87.8 27.9 30.0 13.1 ethanol 2.9 22.6 93.3 46.5 15.0 (1 mg / mL) 27.0 Methanol 1.5 21.6 32.0 54.3 9.7 (1 mg / mL) 33.3 Acetone 0.0 18.9 100.0 77.4 0.0 (1 mg / mL) 7.6 ethyl
acetate 0.0 0.0 100.0 21.6 81.3 (1 mg / mL) 16.1 chloroform 3.1 0.0 100.0 100.0 1.4 (1 mg / mL) 28.0 n-butanol 4.6 13.1 100.0 0.0 1.0 (1 mg / mL) 0.0 n-hexane 0.0 00 92.9 24.7 100.0 16.6

As shown in Table 8, the reducing power of each of the hederock shells obtained in Example 1 was 97.8% to 100% and the ABTS radical scavenging activity was 28.4 to 32.7% in the hot water and high pressure hot water extract, As shown in Fig. The DPPH radical scavenging activity was 82.5 ~ 100% higher than that of methanol extract (32%). Especially acetone, ethyl acetate, chloroform and n - butanol hydroperoxide extract showed 100% DPPH radical scavenging ability Respectively. The activity of metal chelate was 100% in chloroform extract, but not in n - butanol extract. NO scavenging activity was 81.3% in ethyl acetate extract, but acetone, chloroform and n - butanol showed very low activity as 0 ~ 1.4%. In addition, SOD - like activity was the highest in methanol extract.

Production Example 1. Production of softening longevity

The ethanol extract of Example 1 was used to prepare the softening water according to the composition shown in Table 9 below by a conventional method.

ingredient Content (% by weight) Thyme shell extract 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 15 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl vinyl polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Production Example 2. Production of Milk Lotion

Using the ethanol extract of Example 1, milk lotion was prepared according to a conventional method according to the composition shown in Table 10 below.

ingredient Content (% by weight) Thyme shell extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia nut oil 2.5 Polysorbate 60 1.6 Sorbitan sesquiterate 1.6 Propylparaben 0.6 Carboxyl vinyl polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Production Example 3. Nutrition Cream Production

Using the ethanol extract of Example 1, nutritional creams were prepared according to the composition shown in Table 11 below by a conventional method.

ingredient Content (% by weight) Thyme shell extract 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 53 Squalane 3.0 Wax 2.6 Tocopheryl acetate 5.4 Polysorbate 60 3.2 Carboxyl vinyl polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Balance Sum 100

Manufacturing Example 4. Massage Cream Production

Using the ethanol extract of Example 1, a massage cream was prepared according to the composition shown in Table 12 below by a conventional method.

ingredient Content (% by weight) Thyme shell extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl vinyl polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Manufacturing Example 5. Massage Pack Manufacturing

Using the ethanol extract and hot water extract of Example 1, a massage pack was prepared according to the composition shown in Table 13 below.

ingredient Content (% by weight) Thyme shell extract 5.0 Galactomisses fermentation filtrate 1.0 Purified water Balance Carbopol # 940 (2%) 0.1 1,3-butylene glycol 2.0 NATRASOL 250HHR 0.2 Propylene glycol 2.0 Allantoin 0.1 DL-Panthenol 1.0 glycerin 4.0 HC-60 0.3 Complex paraben (PHENONIP) 3.1 Methylparaben (M.P) 1.0 PF (W80402) 0.2 Triethanolamine 2.0 Polygamma glutamic acid (PGA-BG, 1%) 2.1 HYAL-JOINT (1%) 2.0 SKIN SILK OH4 0.5 Aloe extract 1.0 G.P.C 0.1 Ceramide 3.0 Bassam (BSASM) 0.1 Silk protein 0.5

Example 8. Sensory evaluation

Using the mask pack prepared in Preparation Example 5, sensory evaluation was carried out on 30 women in their 20s and 30s, and the results are shown in Table 14 below. At this time, a general mask pack was used as a control group.

Control group Masked pack containing ethanol extract of midduk skin Mask pack containing hydrothermal extract incense 3.77 ± 0.92 2.70 ± 0.99 3.31 ± 0.73 color 3.95 + - 0.74 3.81 ± 0.69 3.78 ± 0.70 Viscosity 3.52 + 0.84 3.63 ± 0.72 3.83 ± 0.81 Brittle 3.86 ± 0.77 3.84 0.76 3.83 ± 0.88 Ease of removal 3.97 ± 0.73 3.89 ± 0.78 3.92 ± 0.67 Absorbency 3.47 ± 0.89 3.28 ± 0.92 3.33 ± 0.91 After application 3.58 ± 0.92 3.50 0.93 3.67 ± 0.82 Moisture power 3.92 ± 0.82 3.81 ± 0.75 3.80 ± 0.82

As shown in Table 14, the sensory evaluation results showed that there was almost no statistically difference between the control group and the mask pack containing the hederock extract. In the case of mask pack using ethanol extract of hot pepper and hot water extract, there was no significant difference in overall, but hot water extract was superior in flavor and viscosity. In addition, there was no statistically significant difference in color, spreadability, ease of removal, absorbency, post-application feeling, and moisturizing ability in the mask pack containing the control group and the hydrothermal extract of the hederock shell, and the viscosity of the mask pack containing the hydrothermal extract .

From the above results, it was found that, in the case of the mask pack containing the hederock bark extract according to the present invention, the hydrothermal extract was superior in the extraction easiness and the consumer survey, and therefore, it is preferable to use the hectorite bark hot water extract.

Although the present invention has been described in terms of the preferred embodiments mentioned above, it is possible to make various modifications and variations without departing from the spirit and scope of the invention. It is also to be understood that the appended claims are intended to cover such modifications and changes as fall within the scope of the invention.

Claims (7)

A cosmetic composition for skin whitening comprising an extract of Chidyuk shell as an active ingredient. The method according to claim 1,
The cosmetic composition for skin whitening according to claim 1, wherein the heder shell extract is extracted with at least one solvent selected from water, ethanol, methanol, acetone, ethyl acetate, chloroform, n-butanol and n-hexane. The method according to claim 1,
The cosmetic composition for whitening skin according to claim 1, wherein the heder skin extract is extracted by at least one method selected from hot water extraction, high-pressure hot water extraction, ethanol extraction, methanol extraction and organic solvent extraction. The method according to claim 1,
The cosmetic composition for whitening skin according to any one of claims 1 to 3, wherein the hairless extract is contained in an amount of 1 to 50% by weight based on the total weight of the composition. The method according to claim 1,
The cosmetic composition is a formulation of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray A cosmetic composition for whitening. The method according to claim 1,
Wherein the cosmetic composition is a mask pack. delete

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