CN106413687B - Product and its preparation method and application containing gadol extract and ginkgo biloba extract - Google Patents
Product and its preparation method and application containing gadol extract and ginkgo biloba extract Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/41—Crassulaceae (Stonecrop family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Abstract
A kind of product and its preparation method and application containing gadol extract and ginkgo biloba extract, the product can inhibit tyrosinase, reduce melanin, the synergistic effect with whitening, be used to prepare skin-lightening cosmetic.
Description
Technical field
The present invention relates to daily chemicals fields, and in particular to a kind of plant extraction liquid containing root of kirilow rhodiola and ginkgo active ingredient and
Application in cosmetics.
Background technique
Pigment in people's autologous skin and hair, function be play the role of protection protect skin or hair from
By the adverse effect of ultraviolet light.If people lacks the care of pigment, skin or hair very sensitive will such as hold sunlight
Easily incur sunburn.Even the age, lighter crowd also had relatively high skin cancers risk.This is because short wavelength UV
Line (wavelength between 290 to 320 nanometers of light) and carcinogen can form harmful free radical such as oxygen radical, these oxygen from
Skin Cell is attacked by base and causes the aging phenomenon of skin.The major function of pigment is to remove harmful free radicals in skin, from
And protect the skin from the infringement of harmful free radicals.Therefore, people, which possesses enough pigments, will possess defence body powerful enough
It is the infringement to protect the skin from physics and chemical toxic substances.Pigmentogenic factor includes three aspects that is, ultraviolet light,
Estrogen and prostaglandin.The generation of melanin is that (dopachrome passes through junket by TYR enzyme catalytic tyrosine generation DOPA
The reaction of propylhomoserin enzyme and the oxidation reaction of a series of complex generate), the melanocyte of generation is transferred in Skin Cell
And the metabolism of adjoint epidermis and fall off.The process that melanin generates is a natural phenomena of normal skin, normally
Dermal melanin it is excessive generation there is no.However, when skin is by from extraneous stimulation such as ultraviolet light, environmental pollution with
And in the presence of pressure factor, the abnormal of dermal melanin is generated and (is generated as excessive) and will occur, when excessive melanin production
Skin does not have relieving capacity to become the pigmentation that will appear skin afterwards.It is ultraviolet in the stimulus that melanin described above generates
Line is the maximum stimulus of melanin biosynthesis and the reaction for influencing melanin production process.In other words,
Ultraviolet light why be melanin production main factor be because ultraviolet light promote chromatophore activity and induce black
Hyperchromatism plays a key role in generating.
It is therein anti-to be that only a kind of enzyme i.e. tyrosinase takes part in for a maximum feature in melanin production mechanism
It answers, thus reasoning, inhibits the activity of tyrosinase that can prevent the generation of melanin, therefore can also be able to the effect of skin-whitening
It realizes.
In patent description before, it is known that have and use ascorbic acid (Vitamin C) as whitening material, however tie up him
Life C has the problem that the problem of its activity can be reduced with the time, this may is that due to formula or technique, technology people
Vitamin C is such as fabricated to capsule or liposome stabilizing active object by the certain methods that member comes up with many stable Vitamin Cs again
The method of matter, however always without a kind of more definite and reliable method.
Quinhydrones is another known whitening material, and effect is than more significant, but the use in cosmetics is limited
System, because it is a kind of carcinogen.
Kojic acid is the relatively good whitening substance of known one kind, due to the ability that it is significantly inhibited tyrosinase activity,
Its whitening effect is excellent, however its stability in formula is difficult to keep, moreover in recent years it was reported that kojic acid is a kind of cause
Cancer object.
In addition to this, it is known that a kind of more commonly used whitening agent be arbutin, arbutin can both be planted by high mountain
A kind of black bearberry tree in extract obtain, can also be obtained by synthetic method.Arbutin has inhibits junket ammonia as kojic acid
The effect of sour enzyme, however be bonded on quinhydrones from arbutin own molecular structure glycan molecule it can be concluded that, when use contains black bearberry
When the product of glycosides, arbutin can be decomposed saccharogenesis and quinhydrones by the enzyme on skin, and quinhydrones herein can generate stimulation to skin.
The plant extracts such as root of Dahurain angelica, saussurea involucrata, Radix Glycyrrhizae, ginkgo, hundreds of plant such as root of kirilow rhodiola, which has, inhibits tyrosinase
Effect, and safety is good, but haves the defects that effect is unobvious or responding time is long.
The present inventor has tested many natural materials for being used to improve cutaneous pigmentation having with physiological activity, as a result
It is a kind of to make root of kirilow rhodiola and the plant extracts of ginkgo have to significantly inhibit tyrosine enzyme activity it was found that in numerous plant extracts
Property and reduce the effect of melanin production, the prior extract for being the discovery that gadol extract and ginkgo is inhibiting tyrosine
The activity aspect of enzyme has preferable synergistic function, will not generate skin irritatin, have the effect of excellent skin whitening,
Meet expection of the invention.
Summary of the invention
A theme of the invention is to provide a kind of plant extraction liquid comprising root of kirilow rhodiola and ginkgo active ingredient, the extracting solution
Have effects that cooperate with whitening.
Another theme of the invention is a Plant Extracts and its application.
A kind of extracting solution containing root of kirilow rhodiola Yu ginkgo active ingredient, component and content are as follows:
(1) the gadol extract dry powder of extracting solution weight 0.03% to 10% is accounted for；
(2) the ginkgo biloba extract dry powder of extracting solution weight 0.01% to 15% is accounted for；
(3) ethyl alcohol of extracting solution weight 50% to 70% is accounted for；
(4) 1,3 butanediols of extracting solution weight 2% to 10% are accounted for；
(5) the EDTA-2 sodium of extracting solution weight 0.15% to 0.3% is accounted for；
(6) surplus is water.
Wherein gadol extract dry powder and ginkgo biloba extract dry powder weight ratio are 3:1 to 1:2.
In extracting solution of the invention, the extraction process of gadol extract dry powder and ginkgo biloba extract dry powder are as follows:
A, the extraction container with condensing unit is cleaned and are put into root of kirilow rhodiola medicinal material or ginkgo medicinal material clear water, extraction is added
Solvent is taken, it is dry medicine that solvent, which is selected from water, ethyl alcohol, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, and the weight of solvent,
1-10 times of material weight；
B, (a) that is proportionally added into extractant is extracted, the temperature heating between 40-50 degrees Celsius is 5 small
When；
B, (b) extracted is filtered with 300 mesh filter screens, and filtered extract is placed in 10 degrees Celsius
At a temperature of and place 8 day time ageing, filtrate is filtered with No. 5 filter paper later；
D, it is dried, (c) after the completion of filtering is subjected to distillation drying using vacuum rotary evaporator, obtains extract
Dry powder.
In extracting solution of the invention, gadol extract dry powder and ginkgo biloba extract dry powder weight ratio are 2.5:1 to 1:
1.5。
In extracting solution of the invention, gadol extract dry powder accounts for extracting solution weight 0.3% to 5%；Ginkgo biloba extract is dry
Powder accounts for extracting solution weight 0.1% to 7.5%.
In extracting solution of the invention, root of kirilow rhodiola medicinal material is the Hua Heye of rhodiola；Ginkgo medicinal material is ginkgo leaf.
In extracting solution of the invention, selected solvent be 70% ethyl alcohol；In extracting solution of the invention, selected is molten
Agent weight is 6 times of dry medicinal material weight.
The application of extracting solution of the invention in skin-lightening cosmetic.
The present invention is beneficial to be the discovery that, when gadol extract and ginkgo biloba extract are with the ratio group of weight ratio 3:1 to 1:2
When conjunction, it is high when can test than its individual event to inhibit tyrosinase activity and reduce the effect of melanin, illustrates that root of kirilow rhodiola extracts
Both object and ginkgo biloba extract act synergistically with whitening.
Specific embodiment
The present invention provides a kind of with the root of kirilow rhodiola for cooperateing with white-skinned face function and ginkgo extract and its application.
Compared with skin-whitening agents before, root of kirilow rhodiola and ginkgo biloba p.e of the present invention all have good inhibition junket ammonia
Phytase activity and the synthesis for inhibiting melanin in chromatophore, root of kirilow rhodiola and ginkgo biloba extract active ingredient have good
Skin whitening effects.
Gadol extract: active ingredient of the root of kirilow rhodiola as lightening compositions in the present invention, root of kirilow rhodiola Latin name
Rhodiola rosea, English name Roseroot are Crassulaceae renascent herb or shrub plant, are grown in height above sea level 800-
The rare wild plant of 2500 meters of high and cold contamination-freely bands.Since its growing environment is severe, as anoxic, low temperature drying, blast, by
Ultraviolet light irradiation, day and night temperature are big, thus have very strong vitality and special adaptability.Traditional medicine is often used for Asia
Material.Know that this platymiscium there are 14 kinds containing rhodioside.The type for making the application of medicinal or health care product at present has: rhodiola
Rhodiola crenulata, root of kirilow rhodiola R.Rosea L, library page root of kirilow rhodiola R.sachalinensis A.Bor., narrow Ye Hongjing
Its R.kirilowii (Regel) Maxim., dark red root of kirilow rhodiola R.coccinea (Royle) A.Bor..Rhodiola mainly at
Part are as follows: rhodioside (Salidroside) and its sweet first tyrosol (Tryosol), 6- oxygen-galloyl rhodioside, 1,2,
Five oxygen of 3,4,6--galloyl-β-D- gives a tongue-lashing glucopyranoside, herbaceous stem element -7- oxygen-α-L- gives a tongue-lashing that Lee's sugar of muttering is sweet, the plain -7- oxygen-of herbaceous stem
It is sweet that (3- oxygen-β-D- gives a tongue-lashing glucopyranoside base)-α-L- gives a tongue-lashing Lee's sugar of muttering；In addition also contain flavonoid glycoside, gallic acid, Kaempferol, quercitrin
Structures such as element, Rosavin (Rosavin), junket raw (Rosarin), junket Sa Li (Rosin) etc..It is sweet containing flavones in root of Rhodiola sachalinensis
Rhodionin, Rhodiosin, Rhodiolin, there are about 30 kinds of volatile oil, and wherein Sosaol content highest, accounts for about 26%, also
β-carypohyllene, α-olive perfume (or spice) alkene etc..Aerial part contains Rhodiolgin, Rhodionidin and Rhodiolgidin etc..
Root of kirilow rhodiola used in the present invention can be derived from the flower of rhodiola, leaf, branch, and advanced optimize as red scape
It Hua Heye.
Gadol extract is one of extracting solution of the present invention active ingredient, extracts the solvent used and is selected from water,
The low-carbon alcohols for being 1 to 4 containing carbon atom number, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, and advanced optimize as second
Alcohol, butanol or methanol, are further optimized for ethyl alcohol, and the ethyl alcohol is the ethyl alcohol that alcoholic strength is 70%.Extractant of the present invention
Usage amount be 1-10 times root of kirilow rhodiola dried flower and cured leaf weight, advanced optimizing is 5-8 times, is further optimized for 6
Times.
The extraction process of gadol extract of the present invention are as follows:
(a) extractant is added in root of kirilow rhodiola dried flower and leaf (weight 1:1), solvent is selected from water, is 1 containing carbon atom number
To 4 low-carbon alcohols, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, and the weight of solvent are 1-10 times of dried flower weight；
(b) (a) that is proportionally added into extractant is extracted, the temperature between 40-100 degrees Celsius heats 3-20
A hour time kept 1-15 days extraction times between 4-40 degrees Celsius of temperature；
(c) (b) extracted is filtered, and filtered extract is subjected to ripening that is, by filtered
Extract be placed at a temperature of 5-10 degrees Celsius and place 7-10 days time ageing, finally again by the extract after ageing into
Row filtering；
(d) it is dried, (c) after the completion of filtering is subjected to distillation drying using vacuum rotary evaporator and obtains dry powder
Extract；
When using 1,3 butanediols as extractant, the bodies lost weight of extract dry weight is controlled percent the present invention
1, because filtered extract is evaporated by rotary evaporator difficult to use.
Gadol extract of the present invention refers to that through above (a), (b), (c), the dry powder (d) obtained after extraction process extracts
Object, therefore following gadol extract is gadol extract dry powder.
Meanwhile the extraction scope of gadol extract of the present invention not only includes the resulting extraction of leach extraction method described above
Object further includes the extract obtained by conventional purification process, for example, Ultra filtration membrane technology is needed to specified molecular weight object
The separation of matter is accepted or rejected, and the application of different chromatographic separation technologies, to the molecular weight of substance, hydrophobicity, hydrophily, charge
Etc. factors take into account.
Gadol extract of the present invention can pass through additional technique such as vacuum distillation technique, freeze drying technology spray drying living
A kind of extract of pulverulence made from technology.
Certain effect, therefore extract institute in application can be presented in use to gadol extract of the present invention on the skin
The carrier needed can be frost or lotion.
The preparation of gadol extract
The embodiment 1 of gadol extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of root of kirilow rhodiola, be added 1.2 liters
Water, then heating extraction, heating and temperature control is at 70-90 degrees Celsius, 5 hours of heating time.Then 300 purposes of extract liquor
Filter device is filtered, filtrate 5-10 degrees Celsius at a temperature of ageing 7-10 days after be filtered with No. 5 filter paper, finally
The filtrate of acquisition is dry by rotary vacuum evaporator, and finally obtains the extract that dry weight is 6 grams.
The embodiment 2 of gadol extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of root of kirilow rhodiola, be added 1.2 liters
Water, then heating extraction, extraction temperature are controlled at 15-35 degrees Celsius, extraction time 5 days.Then the filtering of 300 mesh of extract liquor
Device is filtered, and filtrate further passes through the filtering of 400 mesh filter devices later, and then filtrate is condensed by rotary evaporator
The half (0.6 liter) of original solution, then the ethyl alcohol 0.6 of addition 100% is raised in filtrate, and temperature is controlled at 5-10 degrees Celsius simultaneously
Ageing 7-10 days, last filtrate is filtered with No. 5 filter paper again, and filtered filtrate is dry using rotary vacuum evaporator,
And finally obtain the extract that dry weight is 7 grams.
The embodiment 3 of gadol extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of root of kirilow rhodiola, be added 1.2 liters
Water, then heating extraction, extraction temperature are controlled at 40-50 degrees Celsius, extraction time 5 days.Then the filtering of 300 mesh of extract liquor
Device is filtered, and filtrate temperature control later is aged at 5-10 degrees Celsius, digestion time 7-10 days, is later used filtrate
No. 5 filter paper are filtered, and filtered filtrate is filtered using rotary vacuum evaporator is dry, and finally obtaining dry weight is 6.5 grams
Extract.
The embodiment 4-21 of gadol extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of root of kirilow rhodiola, is separately added into table 1
In cited each 1.2 liters of extractant, then the method for Application Example 3 is extracted, and extraction results are shown in Table 1.
Table 1
The embodiment 22 of gadol extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of root of kirilow rhodiola, be added 1.2 liters
1,3 butanediol extracts 48 hours, and temperature is maintained at 50-60 degrees Celsius, is then filtered with the filter device of 300 mesh, filtrate is in 5-
Filtrate is filtered with No. 5 filter paper after being aged under the conditions of 10 degrees Celsius of temperature 7-10 days, since 1,3 butanediol is as molten
Agent, the bodies lost weight control of extract dry weight is percent 1, therefore the concentration of final extract is 1% (W/V).
The embodiment 23 of gadol extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of root of kirilow rhodiola, be added 1.2 liters
10% ethyl alcohol, then heating 5 hours of extraction, then extract liquor is filtered with the filter device of 300 mesh, later filtrate temperature
Degree control is aged at 5-10 degrees Celsius, digestion time 7-10 days, is later further filtered filtrate with No. 5 filter paper,
Filtered filtrate is dry using rotary vacuum evaporator, and temperature is controlled at 65 degrees Celsius, and finally obtaining dry weight is 8 grams
Extract.
The embodiment 24-32 of gadol extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of root of kirilow rhodiola, is separately added into table 2
In cited each 1.2 liters of extractant, then the method for Application Example 23 is extracted, and extraction results are shown in Table 2.
Table 2
Ginkgo biloba extract
Ginkgo (Ginkgo biloba L.) is Ginkgoaceae, Ginkgo deciduous tree.Before ginkgo appears in several hundred million years, it is
Most ancient Relict Plant, ginkgo leaf ginkgo containing flavones ingredient are double in the gymnosperm carried over after quaternary glacier movement
Flavones (ginkgetin), Isoginkgetin (isoginkgetin), demethyl ginkegetin (bilobetin), rue
Glucoside, Kaempferide -3- scillabiose, Kaempferide, Quercetin, Isorhamnetin (isorhamnetin) etc.；Separately containing bitter taste at
Divide three lactone A of ginkgo (ginkgolide A), B, C and the new lactone A of ginkgo (bilobalide A)；Acrylic component shihimic acid
(shikimic acid), D- saccharic acid (D-glucaric acid), ginkgolic acid (ginkgolic acid) still contain gingko alcohol
(ginnol), bilobanone (ginnone), nonacosane, Octacosanol, hexenal, cupreol, stigmasterol and vitamin etc., and
Containing catechin and epicatechin
Ginkgo biloba extract used in the present invention can be derived from the leaf of ginkgo.
Ginkgo biloba extract is another active ingredient of extracting solution of the present invention, extracts the solvent used selected from water, contains
Having carbon atom number is 1 to 4 low-carbon alcohols, acetone, ethyl acetate, butyl acetate, and advanced optimizing as ethyl alcohol, butanol or first
Alcohol, is further optimized for ethyl alcohol, and the ethyl alcohol is the ethyl alcohol that alcoholic strength is 70%.The usage amount of extractant of the present invention is 1-
10 times of dried flower and the weight of cured leaf, advanced optimizing is 5-8 times, is further optimized for 6 times.
The extraction process of ginkgo biloba extract are as follows:
(a) extractant is added in the cured leaf (weight 1:1) of ginkgo, solvent is selected from water, is 1 to 4 containing carbon atom number
Low-carbon alcohols, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, and the weight of solvent is 1-10 times of dried flower weight.
(b) (a) that is proportionally added into extractant is extracted, the temperature between 40-100 degrees Celsius heats 3-20
A hour time, or 1-15 days extraction times are kept between 4-40 degrees Celsius of temperature, the present invention is preferable over 40-50
Degree Celsius temperature extract and kept for 5 hours of temperature；
(c) (b) extracted is filtered, and filtered extract is subjected to ripening that is, by filtered
Extract be placed at a temperature of 5-10 degrees Celsius and place 7-10 days time ageing, finally again by the extract of Chen Huahou into
Row filtering.
(d) it is dried, (c) after the completion of filtering is subjected to distillation drying using vacuum rotary evaporator and obtains ginkgo
Extract dry powder.
Ginkgo biloba extract of the present invention refers to that through above (a), (b), (c), the dry powder (d) obtained after extraction process extracts
Object, therefore following ginkgo biloba extract is ginkgo biloba extract dry powder.
The preparation of ginkgo biloba extract
The embodiment 1-28 of ginkgo biloba extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of ginkgo leaves, is separately added into table 1
In cited each 1.2 liters of extractant, extraction temperature is controlled at 40-50 degrees Celsius, extraction time 5 days.Then extract liquor is used
The filter device of 300 mesh is filtered, and filtrate temperature control later is aged at 5-10 degrees Celsius, and digestion time 7-10 days,
Filtrate is filtered with No. 5 filter paper later, filtered filtrate is filtered using rotary vacuum evaporator is dry, and is finally obtained
Extraction results are shown in Table 3.
Table 3
The embodiment 19 of ginkgo biloba extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of ginkgo leaves, be added 1.2 liters
10% ethyl alcohol, then heating 5 hours of extraction, temperature control between 50 degrees Celsius to 70 degrees Celsius, and then extract liquor is used
The filter device of 300 mesh is filtered, and filtrate temperature control later is aged at 5-10 degrees Celsius, and digestion time 7-10 days,
Filtrate is further filtered with No. 5 filter paper later, filtered filtrate is dry using rotary vacuum evaporator, temperature control
System finally obtains the extract that dry weight is 5.8 grams at 65 degrees Celsius.
The embodiment 20-28 of ginkgo biloba extract preparation
It will be put into the extraction container with condensing unit after the clear water cleaning, drying of 200 grams of ginkgo leaves, is arranged in addition table 4
Each 1.2 liters of the extractant of act, then heating 5 hours of extraction, temperature control between 50 degrees Celsius to 70 degrees Celsius, then
Extract liquor is filtered with the filter device of 300 mesh, and filtrate temperature control later is aged at 5-10 degrees Celsius, digestion time
7-10 days, filtrate is further filtered with No. 5 filter paper later, filtered filtrate is dry using rotary vacuum evaporator
Dry, temperature is controlled at 65 degrees Celsius, and finally obtains dry powder-shaped extract.Extraction results are shown in Table 4
Table 4
Root of kirilow rhodiola and the plant extracts of ginkgo, which all have, in the present invention inhibits tyrosinase activity and reduces melanin life
At effect, however be more useful the discovery that, when gadol extract and ginkgo biloba extract are with the ratio of weight ratio 3:1 to 1:2
When combination, inhibit tyrosinase activity and reduce melanin effect can than its individual event test when it is high, illustrate root of kirilow rhodiola extract
Both object and ginkgo biloba extract act synergistically with whitening.
Gadol extract and ginkgo biloba extract weight ratio advanced optimize as 2.5:1 to 1:1.5 in the present invention
Efficacy validation 1:
The effect of gadol extract and ginkgo biloba extract inhibit tyrosinase activity
The tyrosine that 40 microlitres of concentration are 1.5 mMs/every liter is taken to be added in sodium phosphate buffer agent (Ph6.8) as matrix
It uses, chooses some embodiment of gadol extract 1-32, some embodiment and first two are extracted in ginkgo biloba extract 1-28
Object is diluted to testing sample solution with 0.05 mole of sodium phosphate respectively by weight the mixture (being shown in Table 5) of 3:1 to 1:1.5.
Each 240 microlitres of the dilute solution of three of the above extract are separately added into 40 microlitres of tyrosine, 20 microlitres of tyrosine is added
Enzyme reacts 15 minutes at a temperature of three kinds of mixed liquors are placed in 37 degrees Celsius later, measures 490 nanometers of absorption peak later,
Corresponding, solution is compared as a control group for a kind of solution without above-mentioned three kinds of extracts, therefore, root
The inhibiting rate that tyrosinase activity is calculated according to equation 1, is shown in Table 5
Inhibitory activity against tyrosinase (%)={ 1-absorbance (comparative group solution)/absorbance (control group solution) } *
100
Table 5
It can be seen from Table 5 that: 10 extract comparisons, gadol extract and silver before root of kirilow rhodiola and ginkgo are chosen respectively
Apricot extract is respectively provided with the good effect for inhibiting tyrosinase.In addition, root of kirilow rhodiola is from ginkgo biloba extract respectively with different heavy
Amount is obviously improved than being combined rear inhibitory activity against tyrosinase.
Efficacy validation 2:
The effect of gadol extract and ginkgo biloba extract inhibit melanin production
Murine melanoma B-16 cell is taken, in the test board of every 12 grid of disk, the B-16 injected is thin for injection
The concentration of born of the same parents is respectively 104, and incubation time is 1 day, and different plant extracts and group are then added in each grid and merges training
Support 3-4 days, later by it is each it is various in culture medium separated with centrifuge.Cell after separation is dense with 500 microlitres of equivalents
It is dissolved in the sodium hydroxide dimethyl sulfoxide solution of degree, then heating 10 minutes in water-bath (90 degrees Celsius).It then again will be molten
Cell after solution be centrifugated and measure supernatant in 570 nanometers of absorbance；As a control group, by B-16 cell according to it
On method processing but do not add any plant extracts.
The inhibiting rate of melanocyte can be calculated by equation 2, see table 6
Melanocyte inhibiting rate (%)={ 1-absorbance (comparative group cell)/absorbance (cellular control unit) } * 100
Table 6
It can be seen from Table 6 that choosing 10 extract comparisons, gadol extract and silver before root of kirilow rhodiola and ginkgo respectively
Apricot extract is respectively provided with good melanin production inhibitory effect.Meanwhile root of kirilow rhodiola from ginkgo biloba extract respectively with different heavy
Amount is obviously improved than being combined rear melanin generating suppression.
The gadol extract obtained using different extracting process, ginkgo biloba extract and the two are with different weight than combining
Extract have the effect of definitely inhibit melanoma cells in melanin growth, the production of cytotoxicity is not observed
It is raw.
In the application in skin-lightening cosmetic, root of kirilow rhodiola has with ginkgo cooperates with beauty for root of kirilow rhodiola and ginkgo biloba extract of the present invention
White effect, and the weight ratio of the two is 2.5:1 to 1:1.5.According to this ratio, root of kirilow rhodiola/ginkgo biloba extract is answered in the present invention
The weight ratio used in is percent 5 to percent 20, and advanced optimizing is percent 5 to percent 15.Using display
Has the effect of fabulous skin-whitening.
Root of kirilow rhodiola/ginkgo extract
The present invention obtains gadol extract dry powder respectively and ginkgo mentions by the extraction above in relation to root of kirilow rhodiola and ginkgo
Take object dry powder, be found through experiments that, when gadol extract and ginkgo biloba extract with extract dry powder weight ratio 3:1 to 1:2's
When ratio combines, inhibits tyrosinase activity and to reduce the effect of melanin high when can test than its individual event, and root of kirilow rhodiola mentions
Object dry powder and ginkgo biloba extract dry powder weight ratio is taken to advanced optimize as 2.5:1 to 1:1.5；
Root of kirilow rhodiola/ginkgo extract of the present invention is by gadol extract dry powder and ginkgo biloba extract dry powder as activity
Composition, and be equipped with solvent combination appropriate and form.
A kind of root of kirilow rhodiola and ginkgo extract with collaboration white-skinned face function of the present invention, component and content are as follows:
(1) the gadol extract dry powder of extracting solution weight 0.03% to 10% is accounted for；
(2) the ginkgo biloba extract dry powder of extracting solution weight 0.01% to 15% is accounted for；
(3) ethyl alcohol of extracting solution weight 25% to 80% is accounted for；
(4) 1,3 butanediols of extracting solution weight 1% to 15% are accounted for；
(5) the EDTA-2 sodium of extracting solution weight 0.15% to 0.3% is accounted for；
(6) surplus is water
A kind of root of kirilow rhodiola and ginkgo extract with collaboration white-skinned face function of the present invention, wherein root of kirilow rhodiola mentions
Object dry powder is taken to account for the 0.3% to 5% of extracting solution weight；Ginkgo biloba extract dry powder account for the 0.1% of extracting solution weight to
7.5%；Ethyl alcohol accounts for the 50% to 70% of extracting solution weight；1,3 butanediols account for the 2% to 10% of extracting solution weight；It is shown in Table 7
Extracting solution of the present invention contains the chelating agent of weight percent 0.05% to 0.5%, and the chelating agent is common for cosmetics
Disodium ethylene diamine tetraacetate (EDTA-2 sodium), advanced optimize to account for extracting solution weight percent 0.15% to 0.3%.
Table 7
Root of kirilow rhodiola/ginkgo extract formula
The application of root of kirilow rhodiola and ginkgo extract in skin-lightening cosmetic
Root of kirilow rhodiola and ginkgo extract will be hereinafter enumerated to be applied in skin-lightening cosmetic with the extracting solution of different weight ratio
Example, such as whitening mask Essence, whitening moisturizing lotion and whitening moisturizer, this citing only be used to prove gadol extract
With the whitening effect of ginkgo biloba extract, the present invention is not limited to this embodiments, as long as in cosmetically acceptable carrier range
All formulas can obtain application.
The present invention is applied to the plant extracts such as gadol extract of cosmetics and ginkgo biloba extract is not with dry powder
Form directly makes an addition in cosmetics, but is applied in cosmetics in the form of extracting solution,
The production method of root of kirilow rhodiola, ginkgo extract:
(1) root of kirilow rhodiola and ginkgo biloba extract dry powder are weighed with weight ratio 3:1 to 1:2 ratio,
(2) (1) weighted extract dry powder is added in ethyl alcohol, is dispersed with stirring and adds 1,3 after completely dissolution
Butanediol
(3) it is eventually adding the water of surplus, and is stirred evenly.
The weight ratio of root of kirilow rhodiola and ginkgo is 3:1 to 1:2, is advanced optimized as 2,5:1 to 1:1.5.
Root of kirilow rhodiola of the present invention and application of the ginkgo whitening extract in cosmetics:
Application of the extracting solution of the present invention in cosmetics, in addition to root of kirilow rhodiola and ginkgo extract are as whitening active composition
It outside, further include some adaptable formula formulations and cosmetically acceptable carrier, some cosmetically acceptable carriers are such as
Conventional use of additive, antioxidant, stabilizer, solubilizer, vitamin, pigment and essence and other vehicle components etc..
Root of kirilow rhodiola of the present invention and ginkgo extract can be applied to conventional arbitrary composite formula form, such as can be
A kind of solution, lotion, adhesive sheet, gel, cream, nutritive water, pulvis, perfumed soap, the cleaning supplies containing surfactant, moisturizing
Oil, powder class foundation cream, emulsion foundation, waxy solid foundation cream is spraying etc., and then the present invention advanced optimizes as, toner, creamy battalion
Nutrient solution, nourishing cream, massage cream, Essence (facial mask), eye cream, cleansing cream, cleaning foam, clean water etc.,
For the form of extracting solution application of the present invention if it is adhesive sheet, cream or gel, cosmetics can receive carrier can
It can be animal fat, vegetable fat, wax, mineral oil, starch, xanthan gum, cellulose derivative, polyethylene glycol, silicone oil, swelling
Soil, silica, talcum powder or zinc oxide.
For the form of extracting solution application of the present invention if it is pulvis or by spraying, it may be cream that cosmetics, which can receive carrier,
Sugar, talcum powder, silica, aluminium hydroxide, calcium silicates, nylon PMPM etc..
If it is viscous toner or lotion, cosmetics can receive carrier and may be the form of extracting solution application of the present invention
Solvent, solubilizer or emulsifier, such as water, ethyl alcohol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Ergol, third
Glycol, fatty acid glyceride, cithrol etc..
For the form of extracting solution application of the present invention if it is suspension, cosmetically acceptable carrier may be liquid diluting
Agent such as water, ethyl alcohol, propylene glycol, a kind of suspending agent such as ethoxylated isostearyl alcohols, polyoxyethylene sorbitan ester or polyoxyethylene are de-
Water sorbitol ester, microcrystalline cellulose, aluminium hydroxide, kaolin, xanthan gum etc..
If the formula of extracting solution application of the present invention is a kind of cleaning compositions containing surfactant, cosmetics can
Receiving carrier may be aliphatic alcohol sulfate, poly alkyl alcohol ether sulfate, sulfosuccinic acid monoesters, isethionate, miaow
Oxazoline derivative, sarcosinate, glycine betaine, fatty alcohol, fatty glyceride, fatty diglycollic amide, vegetable oil, lanolin
Derivative, ethoxylated glycerol aliphatic ester etc..
If the form of extracting solution application of the present invention is a perfumed soap, one kind is containing surfactant or does not contain surface-active
The cleaning compositions of agent, what when use can be erased or be rinsed with water with hygenic towelette.Perfumed soap includes but is not limited to liquid toliet soap, powder
Shape perfumed soap, solid soap box oil soap；The skin cleansing compositions containing surfactant include but is not limited to cleaning foam,
Clean water, cleaning wipes；The skin cleansing compositions without containing surfactant include but is not limited to cleansing cream, cleanser
Liquid, clean water and cleansing gel etc..
The present invention will utilize the overall process of formula Application Example detailed description invention.It must be noted that these are implemented
Only as explanatory explanation or narration of the invention, application range of the invention does not regard this as a limit example.
Application of the present invention in cosmetics, in addition to a kind of root of kirilow rhodiola and ginkgo with collaboration white-skinned face function of the present invention
Extract and related process have restriction outer, and constituting other raw materials in formula Application Example is cosmetically acceptable carrier,
It is formed and weight percent is not influencing to be not construed as limiting in formula technique or the required range of the prior art.
Formula as below Application Example is only used to illustrate a kind of root of kirilow rhodiola with collaboration white-skinned face function contained by the present invention
With percentage of the ginkgo biloba extract in the application of daily formula and its effect of whitening, so that confirming of the invention a kind of has collaboration
Whitening effect possessed by the root of kirilow rhodiola and ginkgo extract of white-skinned face function.
It is formulated Application Example 1
Whitening mask Essence
A kind of root of kirilow rhodiola and ginkgo extract answering in whitening mask Essence with collaboration white-skinned face function of the present invention
With being shown in Table 8
8 whitening mask Essence of table
It is formulated Application Example 2
Whitening and moistening skin lotion
A kind of application of the root of kirilow rhodiola and ginkgo extract with collaboration white-skinned face function of the present invention in whitening and moistening skin lotion,
It is shown in Table 9
9 whitening and moistening skin lotion of table
It is formulated Application Example 3
Whitening moisturizer
A kind of root of kirilow rhodiola and ginkgo extract with collaboration white-skinned face function of invention is in whitening moisturizer
Using being shown in Table 10
10 whitening moisturizer of table
Efficacy validation 3:
A kind of root of kirilow rhodiola and the verifying of ginkgo extract white-skinned face function with collaboration white-skinned face function of the present invention.
The women for selecting 20-40 years old age skin partially black carries out clinical trial (it is required that the unglazed allergies of tester, no makeup
Product allergies), three about 1 square centimeter of position and trace mark, three areas delimited at the arm position of each tester
Position difference (1), which represents, uses the present composition containing extracting solution, and (2) use the present composition for being free of extracting solution, (3)
And blank control, other than blank control region (3) do not smear anything, region (1) and region (2) is smeared wherein respectively
Each cosmetic composition, and the trizonal skin color of skin (L value) is tested after continuously smearing 8 weeks, 8 weeks, tester
Device selects Lab colorimeter system spectral luminosity instrument-Minolta CR-300 to measure the variation using color difference before and after product, and calculates
The changing value (Δ L) of skin coloration, principle are that the melanin in skin is more, and L value is on the contrary bigger with regard to smaller！Therefore Δ L
The more big then effect of color difference is better！
Formulation Example 1
The comparison of whitening mask essence formula of liquid Δ L value and test result, are shown in Table 11
Table 11
Formulation Example 2
The comparison of whitening and moistening skin emulsion formulations Δ L value and test result, are shown in Table 12
Table 12
Formulation Example 3
The comparison of whitening moisturizer Δ L value and test result, are shown in Table 13
Table 13
By the comparison of the above Δ L value and test result, it can be concluded that, the value of chromatism Δ L value of skin is the results show that originally after 8 weeks
Invent it is a kind of with collaboration white-skinned face function root of kirilow rhodiola and ginkgo biloba succi have preferable skin whitening effects.
Claims (6)
1. a kind of extracting solution containing root of kirilow rhodiola and ginkgo active ingredient, component and content are as follows:
(1) the gadol extract dry powder of extracting solution weight 0.03% to 10% is accounted for；
(2) the ginkgo biloba extract dry powder of extracting solution weight 0.01% to 15% is accounted for；
(3) ethyl alcohol of extracting solution weight 50% to 70% is accounted for；
(4) 1,3 butylene glycol of extracting solution weight 2% to 10% is accounted for；
(5) the EDTA-2 sodium of extracting solution weight 0.15% to 0.3% is accounted for；
(6) surplus is water；
Wherein gadol extract dry powder and ginkgo biloba extract dry powder weight ratio are 3:1 to 1:2；
The extraction process of gadol extract dry powder and ginkgo biloba extract dry powder are as follows:
(a) extraction container with condensing unit is cleaned and are put into root of kirilow rhodiola medicinal material or ginkgo medicinal material clear water, extraction is added
Solvent, extractant is selected from water, ethyl alcohol, acetone, ethyl acetate, butyl acetate and 1,3-BDO, and the weight of solvent is
1-10 times of dry medicinal material weight；
(b) (a) that is proportionally added into extractant is extracted, the temperature between 40-50 degrees Celsius heats 5 hours；
(c) (b) extracted is filtered with 300 mesh filter screens, and filtered extract is placed in 10 degrees Celsius
At a temperature of and place 8 day time ageing, filtrate is filtered with No. 5 filter paper later；
D, it is dried, (c) after the completion of filtering is subjected to distillation drying using vacuum rotary evaporator, it is dry to obtain extract
Powder.
2. extracting solution according to claim 1, gadol extract dry powder and ginkgo biloba extract dry powder weight ratio are 2.5:1
To 1:1.5.
3. extracting solution according to claim 1, gadol extract dry powder accounts for extracting solution weight 0.3% to 5%；Ginkgo mentions
Object dry powder is taken to account for extracting solution weight 0.1% to 7.5%.
4. extracting solution according to claim 1, wherein root of kirilow rhodiola medicinal material is the Hua Heye of rhodiola；Ginkgo medicinal material is
Ginkgo leaf.
5. extracting solution according to claim 1, the ethyl alcohol that selected solvent is 70%；Selected weight of solvent is dry
6 times of medicinal material weight.
6. extracting solution described in claim 1 is preparing the application in skin-lightening cosmetic.
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| PCT/CN2015/088733 WO2017035775A1 (en) | 2015-09-01 | 2015-09-01 | Product containing rhodiola rosea extract and ginkgo biloba extract, preparation method therefor, and use thereof |
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| CN112137908A (en) * | 2020-07-31 | 2020-12-29 | 上海澄穆生物科技有限公司 | Application of phenethyl and styryl resorcinol glycoside compounds in melanin generation inhibitor |
| CN116139182A (en) * | 2022-11-28 | 2023-05-23 | 湖北诺克特药业股份有限公司 | Ginkgo leaf extract characterized by shikimic acid and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103142435A (en) * | 2013-03-13 | 2013-06-12 | 广州市天吻娇颜化妆品有限公司 | Whitening composition inhibiting tyrosinase activities with multiple mechanisms, and products of composition |
| CN104027293A (en) * | 2014-06-27 | 2014-09-10 | 王忠良 | Whitening cosmetic and preparation method thereof |
| CN104042468A (en) * | 2014-05-31 | 2014-09-17 | 青岛博泓海洋生物技术有限公司 | Rhodiola-saera whitening skin-care liquid |
| CN104546595A (en) * | 2014-12-17 | 2015-04-29 | 重庆市金渝管道设备有限公司 | Deep penetrating absorption skin care product composition |
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|---|---|---|---|---|
| CN103142435A (en) * | 2013-03-13 | 2013-06-12 | 广州市天吻娇颜化妆品有限公司 | Whitening composition inhibiting tyrosinase activities with multiple mechanisms, and products of composition |
| CN104042468A (en) * | 2014-05-31 | 2014-09-17 | 青岛博泓海洋生物技术有限公司 | Rhodiola-saera whitening skin-care liquid |
| CN104027293A (en) * | 2014-06-27 | 2014-09-10 | 王忠良 | Whitening cosmetic and preparation method thereof |
| CN104546595A (en) * | 2014-12-17 | 2015-04-29 | 重庆市金渝管道设备有限公司 | Deep penetrating absorption skin care product composition |
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