CN106413687A - Product containing Rhodiola extract and Ginkgo biloba extract, and preparation method and use thereof - Google Patents
Product containing Rhodiola extract and Ginkgo biloba extract, and preparation method and use thereof Download PDFInfo
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Abstract
The invention discloses a product containing Rhodiola extract and Ginkgo biloba extract, and a preparation method and use thereof. The product can inhibit tyrosinase, reduce melanin, has a synergistic effect of whitening, and is used for the preparation of whitening cosmetics.
Description
Technical field
The present invention relates to household chemicals field and in particular to a kind of plant extraction liquid containing rhodiola root and ginkgo active ingredient and
Application in cosmetics.
Background technology
Pigment in people's autologous skin and hair, its function is all that the effect playing protection protects skin or hair to avoid
Suffer from the adverse effect of ultraviolet.If people lacks the care of pigment, skin or hair will very sensitive such as hold to sunlight
Easily cause sunburn.Even age lighter crowd also has the high skin cancers risk of comparison.This is because short wavelength UV
Line (wavelength between 290 to 320 nanometers of light) and carcinogenic substance mass-energy are formed with harmful free radical such as oxygen radical, these oxygen oneself
Skin Cell is attacked by base and causes the aging phenomenon of skin.In skin, the major function of pigment is to remove harmful free radicals, from
And protect the skin from the infringement of harmful free radicals.Therefore, people has enough pigments and will have defence body powerful enough
It is to protect the skin from the infringement of physics and chemical toxic substances.Pigmentogenic factor comprises three aspects i.e., ultraviolet,
Estrogen and prostaglandin.Melanic generation is that (dopachrome passes through junket by TYR enzyme catalytic tyrosine generation DOPA
The reaction of propylhomoserin enzyme, and the oxidation reaction generation of series of complex), the melanocyte of generation is transferred in Skin Cell
And the metabolism of adjoint epidermis and come off.The process that melanin produces is a natural phenomena of normal skin, normally
The excessive generation of dermal melanin is not in.However, when skin suffers from from extraneous stimulation such as ultraviolet, environmental pollution with
And in the presence of pressure factor, the exception of dermal melanin produces (producing as excessive) and will occur, when excessive melanin generates
Skin does not have relieving capacity to become the pigmentation that skin occurs afterwards.In the stimulus that the above melanin produces, ultraviolet
Line is the stimulus of the maximum of melanin biosynthesis and has influence on the reaction of melanin generating process.In other words,
Why ultraviolet is that the main factor that melanin generates is because that ultraviolet is promoting chromatophorous activity and inducing black
Hyperchromatism plays the effect of key in generating.
In melanin formation mechanism, to be that only a kind of enzyme is that tyrosinase take part in therein anti-for maximum feature
Should, by this reasoning, suppress the activity of tyrosinase just can stop melanic generation, therefore effect of skin-whitening also can be able to
Realize.
In patent description before it is known that ascorbic acid (Vitamin C) is used as whitening material, but tie up him
Life C has the problem that its activity can reduce over time, and this may is that the problem due to formula or technique, therefore, technology people
Member comes up with many again and stablizes the certain methods of Vitamin C as Vitamin C is fabricated to the stabilizing active thing such as capsule or liposome
The method of matter, but there is no the more definite and reliable method of one kind all the time.
Quinhydrones, is another whitening material known, and its effectiveness comparison is notable, but the use in cosmetics is limited
System, because it is a kind of carcinogen.
Kojic acid, is a kind of known reasonable whitening substance, because it significantly suppresses the ability of tyrosinase activity,
Its whitening effect is excellent, but its stability in formula is difficult to keep, moreover in recent years it was reported that kojic acid is a kind of cause
Cancer thing.
In addition it is known that a kind of the more commonly used whitening agent be ursin, ursin both can be planted by high mountain
A kind of black bearberry tree in extract obtain it is also possible to by synthesis method obtain.Ursin has as kojic acid and suppresses junket ammonia
The effect of sour enzyme, but be bonded in quinhydrones and can draw from ursin its own molecular structure glycan molecule, contain black bearberry when using
During the product of glycosides, ursin can be decomposed saccharogenesis and quinhydrones by the enzyme on skin, and quinhydrones herein can produce to skin and stimulate.
The plant extracts such as root of Dahurain angelica, saussurea involucrata, Radix Glycyrrhizae, ginkgo, hundreds of plant such as rhodiola root has suppression tyrosinase
Effect, and security is good, but there is DeGrain or the defect of responding time length.
The present inventor has tested many natural materials for improving cutaneous pigmentation with physiologically active, result
Find in numerous plant extracts, a kind of plant extracts crying rhodiola root and ginkgo has and significantly inhibits tyrosine enzyme activity
Property and reduce the effect of melanin generation, the prior extract being the discovery that gadol extract and ginkgo is in suppression tyrosine
The active aspect of enzyme has preferable synergistic function, will not produce skin irritatin, has the effect of excellent skin whitening,
Meet the expection of the present invention.
Content of the invention
One theme of the present invention is to provide a kind of plant extraction liquid comprising rhodiola root and ginkgo active ingredient, this extract
There is collaborative whitening effect.
Another theme of the present invention is Plant Extracts and its application.
A kind of extract containing rhodiola root and ginkgo active ingredient, its component and content are：
(1) account for the gadol extract dry powder of extract weight 0.03% to 10%；
(2) account for the ginkgo biloba extract dry powder of extract weight 0.01% to 15%；
(3) account for the ethanol of extract weight 50% to 70%；
(4) 1,3 butanediols of extract weight 2% to 10% are accounted for；
(5) account for the EDTA-2 sodium of extract weight 0.15% to 0.3%；
(6) balance of water.
Wherein gadol extract dry powder and ginkgo biloba extract dry powder weight are than for 3:1 to 1:2.
In the extract of the present invention, the extraction process of gadol extract dry powder and ginkgo biloba extract dry powder is：
A, rhodiola root medicinal material or ginkgo medicinal material clear water are cleaned and put into the extraction container with condensing unit, add extraction
Take solvent, it is dry medicine that solvent is selected from water, ethanol, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, and the weight of solvent
1-10 times of material weight；
B, (a) that be proportionally added into extractant is extracted, the temperature between 40-50 degree Celsius heat 5 little
When；
B, by extraction after (b) filtered with 300 mesh screen packs, and by filter after extract be placed in 10 degrees Celsius
At a temperature of and place 8 day time ageing, afterwards filtrate is filtered with No. 5 filter paper；
(c) after the completion of filtering is carried out distilling drying using vacuum rotary evaporator, obtains extract by d, dried process
Dry powder.
In the extract of the present invention, gadol extract dry powder and ginkgo biloba extract dry powder weight are than for 2.5:1 to 1:
1.5.
In the extract of the present invention, gadol extract dry powder accounts for extract weight 0.3% to 5%；Ginkgo biloba extract is done
Powder accounts for extract weight 0.1% to 7.5%.
In the extract of the present invention, rhodiola root medicinal material is the Hua Heye of rhodiola；Ginkgo medicinal material is ginkgo leaf.
In the extract of the present invention, selected solvent is 70% ethanol；In the extract of the present invention, selected is molten
Agent weight is 6 times of dry medicinal material weight.
Application in skin-lightening cosmetic for the extract of the present invention.
Beneficial being the discovery that of the present invention, when gadol extract compares 3 with ginkgo biloba extract with weight:1 to 1:2 ratio group
During conjunction, its suppression tyrosinase activity and minimizing melanic effect are high when can test than its individual event, illustrate that rhodiola root extracts
Thing and ginkgo biloba extract have whitening synergy.
Specific embodiment
The present invention provides a kind of rhodiola root with collaborative white-skinned face function and ginkgo extract and its application.
Compared with skin-whitening agents before, rhodiola root of the present invention and ginkgo biloba p.e are respectively provided with good suppression junket ammonia
Phytase activity and melanic synthesis in suppression chromatophore, rhodiola root and ginkgo biloba extract active ingredient have good
Skin whitening effects.
Gadol extract：Rhodiola root is as the active ingredient of lightening compositions in the present invention, rhodiola root Latin name
Rhodiola rosea, English name Roseroot, are Crassulaceae renascent herb or shrub plant, are grown in height above sea level 800
The rare wild plant in 2500 meters of high and cold pollution-free areas.Because its growing environment is severe, such as anoxic, low temperature drying, blast, be subject to
Ultraviolet irradiates, day and night temperature is big, thus has very strong vitality and special adaptability.Commonly use traditional medicine for Asia
Material.Known this platymiscium contain rhodioside have 14 kinds.Making medicinal or health products application species at present has：Rhodiola
Rhodiola crenulata, rhodiola root R.Rosea L, storehouse page rhodiola root R.sachalinensis A.Bor., the red scape of narrow leaf
Its R.kirilowii (Regel) Maxim., dark red rhodiola root R.coccinea (Royle) A.Bor..Rhodiola mainly becomes
Part it is：Rhodioside (Salidroside) and its sweet unit tyrosol (Tryosol), 6- oxygen-nutgall anilide rhodioside, 1,2,
3,4,6- five oxygen-nutgall anilide-β-D- gives a tongue-lashing glucopyranoside, herbaceous stem element -7- oxygen-α-L- gives a tongue-lashing that Lee's sugar of muttering is sweet, herbaceous stem plain -7- oxygen -
It is sweet that (3- oxygen-β-D- gives a tongue-lashing glucopyranoside base)-α-L- gives a tongue-lashing Lee's sugar of muttering；In addition flavonoid glycoside, gallic acid, Kaempferol, quercitrin are also contained
Structure such as element, Rosavin (Rosavin), junket raw (Rosarin), junket Sa Li (Rosin) etc..Flavones is contained sweet in root of Rhodiola sachalinensis
Rhodionin, Rhodiosin, Rhodiolin, there are about 30 kinds of volatile oil, wherein Sosaol content highest, account for about 26%, also
β-carypohyllene, α-olive perfume alkene etc..Aerial part contains Rhodiolgin, Rhodionidin and Rhodiolgidin etc..
Used in the present invention, rhodiola root can take from the flower of rhodiola, leaf, branch, and one-step optimization of going forward side by side is red scape
It Hua Heye.
Gadol extract is one of extract of the present invention active ingredient, and it extracts the solvent using and is selected from water,
The low-carbon alcohols being 1 to 4 containing carbon number, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, one-step optimization of going forward side by side is second
Alcohol, butanol or methyl alcohol, are further optimized for ethanol, the ethanol that described ethanol is 70% for alcoholic strength.Extractant of the present invention
Usage amount be 1-10 times of the dried flower of rhodiola root and the weight of cured leaf, be optimized for 5-8 times further, be further optimized for 6
Times.
The extraction process of gadol extract of the present invention is：
A () is by rhodiola root dried flower and leaf (weight 1：1) add extractant, solvent is selected from water, be 1 containing carbon number
To 4 low-carbon alcohols, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, and the weight of solvent are 1-10 times of dried flower weight；
B (a) that be proportionally added into extractant is extracted by (), the temperature heating 3-20 between 40-100 degree Celsius
The time of individual hour or the extraction time keeping 1-15 days between 4-40 degree Celsius of temperature；
C (b) after extracting is filtered by (), and the extract after filtering is carried out ripening i.e., after filtering
Extract is placed at a temperature of 5-10 degree Celsius and places the time ageing of 7-10 days, finally enters the extract after ageing again
Row filters；
D (c) after the completion of filtering is carried out distillation using vacuum rotary evaporator and is dried and obtains dry powder by () dried process
Extract；
When using 1,3 butanediols as extractant, the bodies lost weight of extract dry weight controls percent the present invention
1, because the extract after filtering is evaporated by rotary evaporator difficult to use.
Gadol extract of the present invention refers to that the dry powder obtaining after (d) extraction process extracts by above (a), (b), (c)
Thing, therefore following gadol extract is gadol extract dry powder.
Meanwhile, the extraction scope of gadol extract of the present invention not only includes the extraction of leach extraction method gained described above
Thing, also includes the extract obtaining by conventional purge process, for example, Ultra filtration membrane technology needs to specified molecular weight thing
The separation of matter is accepted or rejected, and the application of different chromatographic separation technology, the molecular weight to material, hydrophobicity, hydrophily, electric charge
Take into account etc. factor.
Gadol extract of the present invention can be by additional technique such as vacuum distillation technique, and freeze drying technology is lived and is spray-dried
A kind of extract of pulverulence that technology is obtained.
Gadol extract of the present invention on skin using certain effect can be assumed, therefore this extract application on institute
The carrier needing can be frost or emulsion.
The preparation of gadol extract
The embodiment 1 of gadol extract preparation
The extraction container with condensing unit will be put into after 200 grams of rhodiola root clear water cleaning, drying, add 1.2 liters
Water, then heating extraction, heating and temperature control at 70-90 degree Celsius, 5 hours of heat time.Then extract 300 purposes
Filter is filtered, and filtrate is filtered with No. 5 filter paper, finally after being aged 7-10 days at a temperature of 5-10 degree Celsius
The filtrate obtaining is dried by rotary vacuum evaporator, and finally obtains the extract that dry weight is 6 grams.
The embodiment 2 of gadol extract preparation
The extraction container with condensing unit will be put into after 200 grams of rhodiola root clear water cleaning, drying, add 1.2 liters
Water, then heating extraction, extraction temperature controls at 15-35 degree Celsius, extraction time 5 days.Then the extract filtration of 300 mesh
Device is filtered, and filtrate passes through 400 mesh filters filtrations further afterwards, and then filtrate is condensed into by rotary evaporator
The half (0.6 liter) of original solution, is subsequently adding 100% ethanol 0.6 and is raised in filtrate, by temperature control at 5-10 degree Celsius simultaneously
Ageing 7-10 days, last filtrate is filtered with No. 5 filter paper again, and the filtrate after filtration is dried through rotary vacuum evaporator again,
And finally obtain the extract that dry weight is 7 grams.
The embodiment 3 of gadol extract preparation
The extraction container with condensing unit will be put into after 200 grams of rhodiola root clear water cleaning, drying, add 1.2 liters
Water, then heating extraction, extraction temperature controls at 40-50 degree Celsius, extraction time 5 days.Then the extract filtration of 300 mesh
Device is filtered, and filtrate temperature control is aged at 5-10 degree Celsius afterwards, and filtrate is used by digestion time 7-10 days afterwards
No. 5 filter paper are filtered, and the filtrate after filtration is dried filter through rotary vacuum evaporator again, and finally obtain dry weight for 6.5 grams
Extract.
Embodiment 4-21 of gadol extract preparation
The extraction container with condensing unit will be put into after 200 grams of rhodiola root clear water cleaning, drying, be separately added into table 1
In cited each 1.2 liters of extractant, then the method for Application Example 3 extracted, extraction results are shown in Table 1.
Table 1
The embodiment 22 of gadol extract preparation
The extraction container with condensing unit will be put into after 200 grams of rhodiola root clear water cleaning, drying, add 1.2 liters
1,3 butanediol, extracts 48 hours, temperature is maintained at 50-60 degree Celsius, is then filtered with the filter of 300 mesh, filtrate is in 5-
Under 10 degrees Celsius of temperature conditionss, filtrate was filtered, because 1,3 butanediol is as molten after 7-10 days by ageing with No. 5 filter paper
Agent, the bodies lost weight of extract dry weight controls percent 1, and the concentration of therefore final extract is 1% (W/V).
The embodiment 23 of gadol extract preparation
The extraction container with condensing unit will be put into after 200 grams of rhodiola root clear water cleaning, drying, add 1.2 liters
10% ethanol, then heating extracts 5 hours, and then extract is filtered with the filter of 300 mesh, filtrate temperature afterwards
Spend to control and be aged at 5-10 degree Celsius, digestion time 7-10 days, afterwards filtrate is filtered with No. 5 filter paper further,
Filtrate after filtration is dried through rotary vacuum evaporator again, and temperature control is at 65 degrees Celsius, and finally acquisition dry weight is 8 grams
Extract.
Embodiment 24-32 of gadol extract preparation
The extraction container with condensing unit will be put into after 200 grams of rhodiola root clear water cleaning, drying, be separately added into table 2
In cited each 1.2 liters of extractant, then the method for Application Example 23 extracted, extraction results are shown in Table 2.
Table 2
Ginkgo biloba extract
Ginkgo (Ginkgo biloba L.), is Ginkgoaceae, Ginkgo deciduous tree.Before ginkgo occurs in several hundred million years, it is
Relict Plant the most ancient in the gymnosperm carrying over after quaternary glacier motion, ginkgo leaf ginkgo containing flavones ingredient is double
Flavones (ginkgetin), Isoginkgetin (isoginkgetin), demethyl ginkegetin (bilobetin), rue
Glucoside, Kaempferide -3- scillabiose, Kaempferide, Quercetin, Isorhamnetin (isorhamnetin) etc.；Separately contain bitter taste to become
Divide ginkgo three lactone A (ginkgolide A), B, C and ginkgo new lactone A (bilobalide A)；Acrylic component shihimic acid
(shikimic acid), D- saccharic acid (D-glucaric acid), ginkgolic acid (ginkgolic acid). still alcohol containing gingko
(ginnol), bilobanone (ginnone), nonacosane, Octacosanol, hexenal, cupreol, stigmasterol and vitamin etc., and
Containing catechin and epicatechin.
Used in the present invention, ginkgo biloba extract can take from the leaf of ginkgo.
Ginkgo biloba extract is another kind of active ingredient of extract of the present invention, and it extracts the solvent using and is selected from water, contains
There are the low-carbon alcohols that carbon number is 1 to 4, acetone, ethyl acetate, butyl acetate, one-step optimization of going forward side by side is ethanol, butanol or first
Alcohol, is further optimized for ethanol, the ethanol that described ethanol is 70% for alcoholic strength.The usage amount of extractant of the present invention is 1-
10 times of dried flower and the weight of cured leaf, are optimized for 5-8 times further, are further optimized for 6 times.
The extraction process of ginkgo biloba extract is：
A () is by the cured leaf (weight 1 of ginkgo：1) add extractant, solvent is selected from water, be 1 to 4 containing carbon number
Low-carbon alcohols, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, and the weight of solvent is 1-10 times of dried flower weight.
B (a) that be proportionally added into extractant is extracted by (), the temperature heating 3-20 between 40-100 degree Celsius
The time of individual hour, or keep the extraction time of 1-15 days between 4-40 degree Celsius of temperature, the present invention is preferable over 40-50
Degree Celsius temperature extraction and 5 hours of keeping temperature；
C (b) after extracting is filtered by (), and the extract after filtering is carried out ripening i.e., after filtering
Extract is placed at a temperature of 5-10 degree Celsius and places the time ageing of 7-10 days, finally enters the extract of Chen Huahou again
Row filters.
D (c) after the completion of filtering is carried out distillation using vacuum rotary evaporator and is dried and obtains ginkgo by () dried process
Extract dry powder.
Ginkgo biloba extract of the present invention refers to that the dry powder obtaining after (d) extraction process extracts by above (a), (b), (c)
Thing, therefore following ginkgo biloba extract is ginkgo biloba extract dry powder.
The preparation of ginkgo biloba extract
Embodiment 1-28 of ginkgo biloba extract preparation
The extraction container with condensing unit will be put into after 200 grams of ginkgo leaf clear water cleaning, drying, be separately added into table 1
In cited each 1.2 liters of extractant, extraction temperature controls at 40-50 degree Celsius, extraction time 5 days.Then extract is used
The filter of 300 mesh is filtered, and filtrate temperature control is aged at 5-10 degree Celsius afterwards, digestion time 7-10 days,
Afterwards filtrate is filtered with No. 5 filter paper, the filtrate after filtration is dried filter through rotary vacuum evaporator again, and finally obtains
Extraction results are shown in Table 3.
Table 3
The embodiment 19 of ginkgo biloba extract preparation
The extraction container with condensing unit will be put into after 200 grams of ginkgo leaf clear water cleaning, drying, add 1.2 liters
10% ethanol, then 5 hours of heating extraction, temperature control between 50 degrees Celsius to 70 degrees Celsius, then use by extract
The filter of 300 mesh is filtered, and filtrate temperature control is aged at 5-10 degree Celsius afterwards, digestion time 7-10 days,
Afterwards filtrate is filtered with No. 5 filter paper further, the filtrate after filtration is dried through rotary vacuum evaporator again, temperature control
System is at 65 degrees Celsius, and finally obtains the extract that dry weight is 5.8 grams.
Embodiment 20-28 of ginkgo biloba extract preparation
The extraction container with condensing unit will be put into after 200 grams of ginkgo leaf clear water cleaning, drying, add in table 4 and arrange
Lift each 1.2 liters of extractant, then heating extraction 5 hours, temperature control between 50 degrees Celsius to 70 degrees Celsius, then
Extract is filtered with the filter of 300 mesh, and filtrate temperature control is aged at 5-10 degree Celsius afterwards, digestion time
7-10 days, afterwards filtrate is filtered with No. 5 filter paper further, the filtrate after filtration is done through rotary vacuum evaporator again
Dry, temperature control is at 65 degrees Celsius, and finally obtains dry powder-shaped extract.Extraction results are shown in Table 4
Table 4
In the present invention, the plant extracts of rhodiola root and ginkgo is respectively provided with suppression tyrosinase activity and reduces melanin life
The effect becoming, but be more useful the discovery that, when gadol extract compares 3 with ginkgo biloba extract with weight:1 to 1:2 ratio
During combination, its suppression tyrosinase activity simultaneously reduces high when melanic effect can be tested than its individual event, illustrates that rhodiola root extracts
Thing and ginkgo biloba extract have whitening synergy.
In the present invention, gadol extract and ginkgo biloba extract weight ratio are optimized for 2.5 further:1 to 1:1.5
Efficacy validation 1：
Gadol extract suppresses effect of tyrosinase activity with ginkgo biloba extract
The tyrosine that 40 microlitres of concentration is 1.5 mMs/every liter is taken to add in sodium phosphate buffer agent (Ph6.8) as matrix
Use, choose certain embodiment of gadol extract 1-32, in ginkgo biloba extract 1-28, certain embodiment and first two are extracted
Thing by weight 3:1 to 1:1.5 mixture (being shown in Table 5) is diluted to testing sample solution with 0.05 mole of sodium phosphate respectively.
Each for the dilute solution of three of the above extract 240 microlitres are separately added into 40 microlitres of tyrosine, add 20 microlitres of tyrosine
Enzyme, three kinds of mixed liquors is placed at a temperature of 37 degrees Celsius reacts 15 minutes afterwards, the absworption peak of 490 nanometers of measurement afterwards,
Corresponding, compare as a control group by solution for a kind of solution without above-mentioned three kinds of extracts, therefore, root
Calculate the inhibiting rate of tyrosinase activity according to equation 1, be shown in Table 5
Inhibitory activity against tyrosinase (%)={ 1 absorbance (comparative group solution)/absorbance (control group solution) } *
100
Table 5
It can be seen from Table 5 that：Choose rhodiola root and ginkgo front 10 extracts contrast, gadol extract and silver respectively
Apricot extract is respectively provided with the effect of good suppression tyrosinase.Additionally, rhodiola root from ginkgo biloba extract respectively with different heavy
Amount is obviously improved than being combined rear inhibitory activity against tyrosinase.
Efficacy validation 2：
Gadol extract and ginkgo biloba extract suppress effect that melanin generates
Take murine melanoma B-16 cell, with the often test board of 12 grid of disk, the B-16 being injected is thin for injection
The concentration of born of the same parents is respectively 104, and incubation time is 1 day, then adds different plant extracts and group to merge training in each grid
Foster 3-4 days, afterwards each culture medium in various is separated with centrifuge.Cell after separating is dense with 500 microlitres of equivalents
Dissolve in the NaOH dimethyl sulfoxide solution of degree, then heating 10 minutes in water-bath (90 degrees Celsius).Then again will be molten
Cell centrifugation after solution separate and measure supernatant in 570 nanometers of absorbance；As a control group, by B-16 cell according to it
On method process but without any plant extracts.
The inhibiting rate of melanocyte can be calculated by equation 2, asks for an interview table 6
Melanocyte inhibiting rate (%)={ 1 absorbance (comparative group cell)/absorbance (cellular control unit) } * 100
Table 6
It can be seen from Table 6 that, choose rhodiola root and ginkgo front 10 extracts contrast, gadol extract and silver respectively
Apricot extract is respectively provided with good melanin and generates inhibition.Meanwhile, rhodiola root from ginkgo biloba extract respectively with different heavy
Amount is obviously improved than being combined rear melanin generating suppression.
Using different extracting process obtain gadol extract, ginkgo biloba extract and both with Different Weight than combination
Extract there is the effect of melanic growth in definite suppression MC, the product of cytotoxicity is not observed
Raw.
, in the application in skin-lightening cosmetic, rhodiola root and ginkgo have collaborative U.S. for rhodiola root of the present invention and ginkgo biloba extract
White effect, and both weight is than for 2.5:1 to 1:1.5.According to this ratio, rhodiola root/ginkgo biloba extract should in the present invention
With used in weight ratio for 5 to percent 20 percent, one-step optimization of going forward side by side is 5 to percent 15 percent.Application display
There is the effect of fabulous skin-whitening.
Rhodiola root/ginkgo extract
The present invention passes through the extraction above in relation to rhodiola root and ginkgo, obtains gadol extract dry powder respectively and ginkgo carries
Take thing dry powder, be found through experiments, when gadol extract compares 3 with ginkgo biloba extract with extract dry powder weight:1 to 1:2
During ratio combination, its suppression tyrosinase activity simultaneously reduces high when melanic effect can be tested than its individual event, and rhodiola root carries
Thing dry powder is taken to be optimized for 2.5 further with ginkgo biloba extract dry powder weight ratio:1 to 1:1.5；
Rhodiola root/ginkgo extract of the present invention is to be used as activity by gadol extract dry powder and ginkgo biloba extract dry powder
Composition, and be equipped with suitable solvent combination and form.
A kind of rhodiola root with collaborative white-skinned face function of the present invention and ginkgo extract, its component and content are：
(1) account for the gadol extract dry powder of extract weight 0.03% to 10%；
(2) account for the ginkgo biloba extract dry powder of extract weight 0.01% to 15%；
(3) account for the ethanol of extract weight 25% to 80%；
(4) 1,3 butanediols of extract weight 1% to 15% are accounted for；
(5) account for the EDTA-2 sodium of extract weight 0.15% to 0.3%；
(6) balance of water
A kind of rhodiola root with collaborative white-skinned face function of the present invention and ginkgo extract, wherein rhodiola root carries
Thing dry powder is taken to account for the 0.3% to 5% of extract weight；Ginkgo biloba extract dry powder account for the 0.1% of extract weight to
7.5%；Ethanol accounts for the 50% to 70% of extract weight；1,3 butanediols account for the 2% to 10% of extract weight；It is shown in Table 7
Extract of the present invention contains the chelating agent of percentage by weight 0.05% to 0.5%, and this chelating agent is commonly used for cosmetics
Disodium ethylene diamine tetraacetate (EDTA-2 sodium), be optimized for further accounting for extract percentage by weight 0.15% to 0.3%.
Table 7
Rhodiola root/ginkgo extract formula
Rhodiola root and application in skin-lightening cosmetic for the ginkgo extract
Hereinafter it is applied to enumerating rhodiola root in skin-lightening cosmetic with the extract of Different Weight ratio with ginkgo extract
Example, such as cleawhite pack Essence, whitening moisturizing lotion and whitening moisturizer, this citing is only for proving gadol extract
With the whitening effect of ginkgo biloba extract, the invention is not restricted to this embodiment, as long as in cosmetically acceptable carrier scope
All formulas can obtain application.
It is not with dry powder that the present invention is applied to the plant extracts such as gadol extract of cosmetics and ginkgo biloba extract
Form directly makes an addition in cosmetics, but is applied in the form of extract in cosmetics,
Rhodiola root, the preparation method of ginkgo extract：
(1) rhodiola root and ginkgo biloba extract dry powder are compared 3 with weight:1 to 1:2 ratios are weighed,
(2) extract dry powder weighted to (1) is added in ethanol, after dispersed with stirring fully dissolving, adds 1,3
Butanediol
(3) it is eventually adding the water of surplus, and stir.
The weight of rhodiola root and ginkgo is than for 3:1 to 1:2, it is optimized for 2,5 further:1 to 1:1.5.
Rhodiola root of the present invention and application in cosmetics for the ginkgo whitening extract：
Application in cosmetics for the extract of the present invention, except rhodiola root and ginkgo extract are as whitening active composition
Outward, some adaptable formula formulations and cosmetically acceptable carrier are also included, some cosmetically acceptable carriers are such as
Conventional use of additive, antioxidant, stabilizer, solubilizer, vitamin, pigment and essence, and other vehicle component etc..
Rhodiola root of the present invention and ginkgo extract can apply to the arbitrary composite formula form of routine, can be such as
A kind of solution, emulsion, sticking tablet, gel, cream, nutritive water, pulvis, perfumed soap, the cleaning supplies containing surfactant, moisturizing
Oil, powder class foundation cream, emulsion foundation, waxy solid foundation cream, spraying etc., then the present invention is optimized for further, toner, creamy battalion
Nutrient solution, nourishing cream, massage cream, Essence (facial mask), eye cream, cleansing cream, cleaning foam, clean water etc.,
The form of extract of the present invention application if sticking tablet, cream or gel, its cosmetics can receive carrier can
Can be animal fat, vegetable fat, wax, mineral oil, starch, xanthan gum, cellulose derivative, polyethylene glycol, silicone oil, swelling
Soil, silica, talcum powder or zinc oxide.
If pulvis or spraying, it is probably breast that its cosmetics can receive carrier to the form of extract application of the present invention
Sugar, talcum powder, silica, aluminium hydroxide, calcium silicates, nylon PMPM etc..
If viscous toner or emulsion, its cosmetics can receive carrier and be probably the form of extract application of the present invention
Solvent, solubilizer, or emulsifying agent, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, phenmethylol, Ergol, third
Glycol, fatty acid glyceride, cithrol etc..
If suspension, its cosmetically acceptable carrier is probably liquid diluting to the form of extract application of the present invention
Agent such as water, ethanol, propane diols, a kind of suspending agent such as ethoxylated isostearyl alcohols, polyoxyethylene sorbitan ester or polyoxyethylene take off
Water sorbitol ester, microcrystalline cellulose, aluminium hydroxide, kaolin, xanthan gum etc..
If the formula of extract application of the present invention is a kind of Cleasing compositions containing surfactant, its cosmetics can
Receiving carrier is probably aliphatic alcohol sulfate, poly alkyl alcohol ether sulfate, sulfosuccinic acid monoesters, isethionate, miaow
Oxazoline derivative, sarcosinate, glycine betaine, fatty alcohol, fatty glyceride, fatty diglycollic amide, vegetable oil, lanolin
Derivative, ethoxylated glycerol fatty acid ester etc..
If the form of extract application of the present invention is a perfumed soap, one kind containing surfactant or does not contain surface-active
The Cleasing compositions of agent, can be erased with hygenic towelette during use or with water rinse.Perfumed soap includes but is not limited to liquid toliet soap, powder
Shape perfumed soap, solid soap box oil soap；The described skin cleansing compositions containing surfactant include but is not limited to cleaning foam,
Clean water, cleaning wipes；The described skin cleansing compositions not containing surfactant include but is not limited to cleansing cream, cleanser
Liquid, clean water and cleansing gel etc..
The present invention will describe the overall process of invention in detail using formula Application Example.It must be noted that these enforcements
Example is only used as the explanatory explanation of the present invention or narration, and the range of application of the present invention does not regard this as a limit.
Application in cosmetics for the present invention, except the rhodiola root with collaborative white-skinned face function a kind of to the present invention and ginkgo
Extract and related process have restriction outer, and constituting the other raw materials in formula Application Example is cosmetically acceptable carrier,
Its composition and percentage by weight be not affecting to be not construed as limiting in formula technique or the required scope of prior art.
Formula as below Application Example is only used for a kind of rhodiola root with collaborative white-skinned face function contained by the present invention is described
With percentage in daily formula is applied for the ginkgo biloba extract and its whitening effect, thus confirm one kind of the present invention have collaborative
The whitening effect that the rhodiola root of white-skinned face function and ginkgo extract have.
Formula Application Example 1
Cleawhite pack Essence
A kind of rhodiola root with collaborative white-skinned face function of the present invention and ginkgo extract answering in cleawhite pack Essence
With being shown in Table 8
Table 8 cleawhite pack Essence
Formula Application Example 2
Whitening and moistening skin emulsion
The application in whitening and moistening skin emulsion of a kind of rhodiola root with collaborative white-skinned face function of the present invention and ginkgo extract,
It is shown in Table 9
Table 9 whitening and moistening skin emulsion
Formula Application Example 3
Whitening moisturizer
A kind of rhodiola root with collaborative white-skinned face function of invention and ginkgo extract are in whitening moisturizer
Application, is shown in Table 10
Table 10 whitening moisturizer
Efficacy validation 3：
A kind of rhodiola root with collaborative white-skinned face function of the present invention and the checking of ginkgo extract white-skinned face function.
Select age 20-40 year the partially black women of skin carry out clinical trial and (require the unglazed allergies of tester, no make up
Product allergies), three about 1 square centimeter of position trace mark, three areas delimited at the arm position of each tester
(1) represents using the present composition containing extract respectively for position, and (2) are using the present composition without extract, (3)
And blank, in addition to blank region (3) do not smear anything, region (1) and region (2) smears wherein respectively
Each cosmetic composition, and continuously smear 8 weeks, the test trizonal skin color of skin (L value), tester after 8 weeks
Device measures the change using aberration before and after product from Lab colorimeter system spectral luminosity instrument-Minolta CR-300, and calculates
The changing value (Δ L) of skin colourity, its principle is that the melanin in skin is more, and L value is less, otherwise bigger！Therefore Δ L
The more big then effect of aberration is better！
Formulation Example 1
The contrast of cleawhite pack elite formula of liquid Δ L value and test result, are shown in Table 11
Table 11
Formulation Example 2
The contrast of whitening and moistening skin emulsion formulations Δ L value and test result, are shown in Table 12
Table 12
Formulation Example 3
The contrast of whitening moisturizer Δ L value and test result, are shown in Table 13
Table 13
Can show, after 8 weeks, the value of chromatism Δ L value result of skin shows by the contrast of above Δ L value and test result, this
A kind of rhodiola root with collaborative white-skinned face function of invention has preferable skin whitening effects with ginkgo biloba succi.
Claims (7)
1. a kind of extract containing rhodiola root and ginkgo active ingredient, its component and content are：
(1) account for the gadol extract dry powder of extract weight 0.03% to 10%；
(2) account for the ginkgo biloba extract dry powder of extract weight 0.01% to 15%；
(3) account for the ethanol of extract weight 50% to 70%；
(4) 1,3 butanediols of extract weight 2% to 10% are accounted for；
(5) account for the EDTA-2 sodium of extract weight 0.15% to 0.3%；
(6) balance of water.
Wherein gadol extract dry powder and ginkgo biloba extract dry powder weight are than for 3:1 to 1:2.
2. extract according to claim 1, the extraction process of gadol extract dry powder and ginkgo biloba extract dry powder is：
A, rhodiola root medicinal material or ginkgo medicinal material clear water are cleaned and put into the extraction container with condensing unit, add extraction molten
Agent, it is dry medicinal material weight that solvent is selected from water, ethanol, acetone, ethyl acetate, butyl acetate and 1,3 butanediols, and the weight of solvent
1-10 times of amount；
B, (a) that be proportionally added into extractant is extracted, the temperature between 40-50 degree Celsius heats 5 hours；
C, (b) after extraction is filtered with 300 mesh screen packs, and the extract after filtering is placed in 10 degrees Celsius of temperature
Degree is lower and places the ageing of 8 day time, is filtered filtrate with No. 5 filter paper afterwards；
(c) after the completion of filtering is carried out distilling drying by d, dried process using vacuum rotary evaporator, obtains extract and does
Powder.
3. extract according to claim 1, gadol extract dry powder and ginkgo biloba extract dry powder weight are than for 2.5:1
To 1:1.5.
4. extract according to claim 1, gadol extract dry powder accounts for extract weight 0.3% to 5%；Ginkgo carries
Thing dry powder is taken to account for extract weight 0.1% to 7.5%.
5. extract according to claim 2, rhodiola root medicinal material is the Hua Heye of rhodiola；Ginkgo medicinal material is ginkgo
Leaf.
6. extract according to claim 2, selected solvent is 70% ethanol；Selected weight of solvent is dry
6 times of medicinal material weight.
7. application in skin-lightening cosmetic for the extract described in claim 1.
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| PCT/CN2015/088733 WO2017035775A1 (en) | 2015-09-01 | 2015-09-01 | Product containing rhodiola rosea extract and ginkgo biloba extract, preparation method therefor, and use thereof |
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| CN109232680A (en) * | 2018-08-15 | 2019-01-18 | 康美保宁（四川）制药有限公司 | A kind of extracting method of root of kirilow rhodiola |
| CN116139182A (en) * | 2022-11-28 | 2023-05-23 | 湖北诺克特药业股份有限公司 | Ginkgo leaf extract characterized by shikimic acid and preparation method thereof |
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| CN112137908A (en) * | 2020-07-31 | 2020-12-29 | 上海澄穆生物科技有限公司 | Application of phenethyl and styryl resorcinol glycoside compounds in melanin generation inhibitor |
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| CN116139182A (en) * | 2022-11-28 | 2023-05-23 | 湖北诺克特药业股份有限公司 | Ginkgo leaf extract characterized by shikimic acid and preparation method thereof |
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