KR101061722B1 - Cosmetic composition for skin whitening containing light green color extract as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening containing a pale green extract as an active ingredient. The present invention also relates to a cosmetic method characterized in that the cosmetic composition is applied to human skin. According to the present invention, pale yellow extract has a melanin production inhibitory effect and tyrosinase activity inhibitory effect, it was confirmed that it has an excellent effect in the whitening clinical experiment of the formulation containing it. Therefore, the cosmetic composition of the present invention comprising the light green color extract having various functions as an active ingredient has a skin whitening effect.

Light green color, skin whitening, cosmetic composition

Description

Cosmetic composition for skin whitening containing light green extract as an active ingredient {Cosmetic Composition for Whitening of the Skin Comprising the Extract of Corydalis yanhusuo as Active Ingredient}

The present invention relates to a cosmetic composition for skin whitening containing light green color as an active ingredient. The present invention also relates to a cosmetic method characterized in that the cosmetic composition is applied to human skin.

Factors that determine skin color are basically the areas such as tanning due to blemishes, freckles and sun exposure, or overall pigmentation, other than acne, scars, keratin distribution, Blood circulation, stress, and health. Among the above factors, the most important factor is pigmentation.

The pigments that affect skin color include melanin, melazoids, carotene, and hemoglobin, the most important of which is melanin. The biggest factor affecting the biosynthesis of melanin is ultraviolet rays and hormones in the body. Melanin plays an important role in preventing or damaging the skin from ultraviolet rays by absorbing or scattering ultraviolet rays. In addition, it is excellent in removing the active oxygen species, but sometimes melanin itself generates free radicals, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows the properties of free radicals. Pray.

The biosynthesis of melanin is a series of oxidation processes, starting with the conversion of tyrosine, an amino acid, from the melanocytes of melanocytes to tyrosinase and dihydroxy phenylalanine. eumelanin). This biosynthesis process is carried out in a special type of brown intracellular organelles called melanosomes. Melanosomes, including melanin granules, move from the periphery of the nucleus to the dendritic end of the dendritic projections and into the cytoplasm by the phagocytosis of keratinocytes. Accumulate around the nucleus.

The synthesis of melanin, the number of melanosomes, and the migration to the surrounding keratinocytes are partially influenced by hormones and ultraviolet rays and primarily by genetic effects. In addition, it is known that metal ions such as cytokines, copper, zinc, and iron, interferon, prostaglandin, histamine, etc., which are involved in the expression of tyrosinase, the synthesis and transfer of melanin, are involved.

Ascorbic acid (JP-A 4-9320), hydroquinone (JP-A-192062), kojic acid (JP-A-56-7710), arbutin (JP-A 4-93150) and some extracts inhibit tyrosinase. Although it is used as a whitening cosmetic because of its activity, the stability in cosmetic formulations is low, so it is degraded and colored, or its use is limited due to the occurrence of off-flavor, efficacy at the biological level, unclear effect and safety problems. And the inhibitory effect of tyrosinase has been demonstrated, but the effect is low in experiments similar to the actual biologic level. Therefore, the evaluation of the inhibitory effect by melanoma cell culture similar to the biological level is required. In addition, hydroquinone is defined as a carcinogenic substance and its use is prohibited or restricted in cosmetics. Kojic acid and ascorbic acid are very unstable substances. Cosmetics containing a small amount of the components have a problem such as browning occurs when stored for several weeks at room temperature.

Throughout this specification, numerous patent documents and articles are referenced and their citations are indicated. The disclosures of cited patent documents and articles are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors searched various skin physiological activities of natural plant components to improve skin pigmentation, and the light green extract of poppy and perennial herb among natural plant extracts has an excellent inhibitory effect on tyrosinase activity and melanin production, and skin irritation. The present invention was completed by confirming that the skin whitening improvement effect is excellent without it.

Therefore, it is an object of the present invention to provide a cosmetic composition for skin whitening containing light green color extract as an active ingredient.

Another object of the present invention is to provide a cosmetic method comprising applying a cosmetic composition containing the pale green extract to human skin.

Other objects and advantages of the present invention will become apparent from the following examples and claims.

The present invention relates to a cosmetic composition for skin whitening containing a pale green extract as an active ingredient. According to the present invention, the pale green extract has a melanin production inhibitory effect (Experimental Example 1), tyrosinase activity inhibitory effect (Experimental Example 2). Therefore, the cosmetic composition of the present invention comprising the light green color extract having various functions as an active ingredient has an excellent skin whitening effect.

Light green color ( Corydalis yanhusuo , Corydalis turtschaminovii ) is a perennial herbaceous grass 20 Cm high, leaves alternate, long oval. In April-May, light red-purple flowers bloom in inflorescences and bear fruit. Tuber is used as a herbal medicine and grows in mountains or fields. It is distributed in Korea, Manchuria and Siberia. In Chinese medicine, blood circulation is well circulated, so it is used for bruises, blood, menstrual impurity, etc. In oriental medicine, it is used for blood, ecchymosis, iso stool, and diarrhea. In addition, the ingredients are known as Berberine, columbamine, Coptisine, Corlumidine, Glaucine, Stylopine.

As used herein, the term "pure yellow extract" means that obtained by extracting from various organs or parts of pale yellow (e.g., leaves, flowers, roots, stems, branches, shells and seeds, etc.), preferably leaves, stems , Branch, bark or root, more preferably stem, branch or root, most preferably means an extract obtained from the stem.

The pale green extract of the present invention may be prepared according to conventional methods known in the art, that is, under the conditions of ordinary temperature and pressure, using conventional solvents, preferably purified water, methanol, ethanol, Extracted using at least one extraction solvent selected from the group consisting of glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol and hexane, more preferably ethanol, 70% Extracted using ethanol or water, most preferably extracted using 70% ethanol.

On the other hand, it will be apparent to those skilled in the art that the pale green extract of the present invention can obtain an extract having substantially the same effect using not only the aforementioned extraction solvent but also other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using ultrafiltration membranes with constant molecular weight cut-off values, various chromatography (size, charge, hydrophobicity or affinity) The active fraction obtained through various purification methods additionally carried out, such as separation by sex)) is also included in the extract of the present invention.

In another aspect, the present invention provides a composition for external application for skin, characterized in that the extract is obtained by cold condensation at room temperature, heat filtration, and further obtained by concentrating the solvent under reduced pressure or lyophilization.

In addition, the pale green extract of the present invention is contained 0.001-30.0% by weight, preferably 0.01-10.0% by weight based on the total cosmetic composition. When the content of the extract is less than 0.001% by weight, the whitening effect is extremely weak. When the content of the extract is more than 30.0% by weight, the effect of increasing the content is insignificant, and the stability of the formulation is not economical.

The pale green extract of the present invention can achieve its effect by topical application or spraying on the skin. Therefore, according to one preferred aspect of the present invention, the composition of the present invention may be prepared in the form of cosmetic compositions such as creams, lotions and softening water or pharmaceutical compositions such as sprays and ointments.

Ingredients included in the cosmetic composition of the present invention as an active ingredient includes components conventionally used in cosmetic compositions in addition to the active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays may be formulated, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the present invention provides a cosmetic method comprising applying a cosmetic composition containing the pale green extract of the present invention to the human skin.

The cosmetic method of the present invention refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all the methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin protection effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is cleansing Creams, cleansing lotions, cleansing water, and cleansing gels.

When the cosmetic method including the light green extract of the present invention is applied to the skin of a human, a cosmetic whitening effect can be obtained.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Preparation Example 1. Preparation of pale yellow extract

The thin, pale green stems were chopped and refluxed three times for 5 hours with a 70% (V / V) ethanol aqueous solution, and then cooled and then filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C or lower. The light green extract was prepared by dispersing / dissolving in a 50% butylene glycol solution under reduced pressure and freeze-dried 1%.

Experimental Example 1: Determination of melanin production inhibitory effect using B16F1 melanogenesis cells

In order to confirm the whitening effect of the pale green extract obtained in Preparation Example 1, the whitening effect was determined by looking at the degree of inhibition of melanin production on B16F1 melanocytes. B16F1 melanocytes used in this experiment is a cell strain derived from the mouse, a cell that secretes a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. B16F1 melanocytes used in the present experiment was used by receiving from the American Type Culture Collection (ATCC).

The melanin biosynthesis inhibitory effect of B16F1 melanogenesis cells was measured as follows. B16F1 melanocytes were dispensed in 6-well plates at a concentration of 2 X 10 ⁶ per well, and the cells were attached and incubated for 72 hours by treating the sample at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, the cell number was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out with a slight modification of the method of Rotan: Cancer Res., 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. Melanin was extracted by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), and then the absorbance of melanin was measured at 405 nm with a microplate reader. Production inhibition rate (%) was measured. Melanin inhibition rate (%) of B16F1 melanogenesis cells was calculated by Equation 1, the values are shown in Table 1 below. IC ₅₀ value is the concentration of substance that inhibits melanin production by 50%. In order to confirm the degree of melanin inhibition rate of pale yellow extract, the same experiment was conducted using hydrinone and arbutin as positive controls.

A: Melanin amount in wells without sample

B: Melanin amount in the wells to which the sample was added

Sample Name Treatment concentration (ppm) Melanin production inhibition rate (%) Light green color extract (Production example 1) 10 11.14 Light green color extract (Production example 1) 20 32.67 Light green color extract (Production example 1) 50 57.99 Light green color extract (Production example 1) 100 77.56 Light green color extract (Production example 1) 200 83.22 Light green color extract (Production example 1) 400 85.7 Hydroquinone 0.1% 60.4 Arbutin 0.1% 60.4

As shown in the results of Table 1, the melanin production inhibitory effect (IC ₅₀ ) of the pale green extract of the present invention is very low at about 50 ppm, melanin production of the comparative example using 0.1% of hydroquinone and arbutin, respectively, positive control Compared with the inhibition rate, the melanin production inhibition rate of the pale green extract of the present invention was found to be superior even at a much lower concentration.

Experimental Example 2 Measurement of Inhibition of Tyrosinase Activity

Tyrosinase was isolated and purified from mushrooms and purchased from Sigma (United States of America). L-tyrosine (Sigma) was used as a substrate by dissolving in 0.05 M sodium phosphate buffer (pH 6.8) to make a 0.1 mg / ml solution. The test substance was used after dissolving to a suitable concentration in 0.05M sodium phosphate buffer (pH 6.8). Light green color extract was prepared and used according to the method of Preparation Example 1. 0.5 ml of L-tyrosine solution was placed in a test tube, and 0.5 ml of sample solution was added thereto, and the mixture was left to stand at 37 ° C. for 10 minutes. Thereafter, 0.5 ml of 200 unit / ml tyrosinase was added to each sample solution, and the mixture was reacted at 37 ° C for 10 minutes. The test tube containing the reaction solution was placed on ice, quenched to stop the reaction, and the absorbance at wavelength 475 nm was measured with a spectrophotometer. As a control, 0.5 ml of buffer solution was used instead of the sample solution.

The tyrosinase activity inhibition rate of each sample solution was calculated according to Equation 2 below and the experimental results are shown in Table 2 below.

Light green extract (µg / ml) Enzyme Inhibition Rate (%) 5 22.5 10 33.8 20 40.1 50 50.9 100 60.3 200 77.8

As can be seen from the results of Table 2, it was confirmed that the effect of inhibiting tyrosinase activity of pale yellow extract.

Hereinafter, in the formulation example, but used pale green extract prepared from Preparation Example 1 of the present invention is not limited thereto.

Formulation Example 1 Flexible Lotion (Skin)

Formulation examples of the flexible lotion (skin) in the cosmetic composition containing the pale green extract of the present invention is as follows:

ingredient Content (% by weight) Pale yellow extract of Preparation Example 1 1.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 2. Nutrients (Milk Lotion)

An example of the formulation of nutrient longevity (milk lotion) in the cosmetic composition containing the light green extract of the present invention is as follows:

ingredient Content (% by weight) Pale yellow extract of Preparation Example 1 2.0 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 3. Nutrition Cream

An example of the formulation of the nourishing cream in the cosmetic composition comprising the light green extract of the present invention is as follows:

ingredient Content (% by weight) Light green extract 3.0 Lipophilic monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Preservative, fragrance, coloring a very small amount Distilled water Balance Sum 100

Formulation Example 4 Massage Cream

An example of the formulation of the massage cream in the cosmetic containing the extract of pale red mud of the present invention is as follows:

ingredient Content (% by weight) Pale yellow extract of Preparation Example 1              0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 5 Pack

Formulation examples of the pack in the cosmetic composition comprising the pale green extract of the present invention is as follows:

ingredient Content (% by weight)  Pale yellow extract of Preparation Example 1 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Balance system 100

Experimental Example  3: Measure the whitening effect

30 females with dark skin color were used in the nutritional cream of Formulation Example 3 containing Cheoncho extract of the present invention and Comparative Example using 1,3-BG instead of pale red extract in Formulation Example 3. The whitening effect of the cosmetic was measured. Twelve positions of the arm of the subject were displayed by 1 cm in width and length, respectively, and the cosmetics of Formulation Example 3 and Comparative Example were applied, and the other one was used as a control. Cosmetics were applied twice a day for two months. Skin color (L value) was measured after 2 months using Minolta CR 300 as a measuring device, and the change in skin color (ΔL value) was calculated using Equation 3 below and the results are shown in Table 8.

Skin color gradient division Comparative example Formulation Example 3 ΔL value 0.86 4.23

As can be seen from the results of Table 8, the skin color change (ΔL) value of Formulation Example 3 containing the pale red extract was significantly increased compared to the comparative example without the pale red extract. As a result, the whitening effect of the cosmetic containing the pale yellow extract of this invention was confirmed.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (5)

Cosmetic composition for skin whitening containing light green color ( Corydalis yanhusuo , Corydalis turtschaminovii) extract as an active ingredient. The cosmetic composition according to claim 1, wherein the pale green extract is contained in an amount of 0.001-30.0 wt% based on the total weight of the composition. According to claim 1, wherein the pale green extract is an extract solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene glycol Cosmetic composition characterized in that extracted by using the solvent extraction method used. The composition of claim 1 wherein the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition, characterized in that it has a formulation selected from the group. delete